{"title":"Essential oils of Citrus limon, Cymbopogon citratus, and Lavandula officinalis disrupt E. coli biofilms by inducing cellular damage.","authors":"Aalia Khanem, Naheed Karim, Ikram Ullah, Farhan Younas","doi":"10.1007/s42977-025-00255-5","DOIUrl":"https://doi.org/10.1007/s42977-025-00255-5","url":null,"abstract":"<p><p>Biofilms by E. coli is not only the primary cause of recurrent urinary tract infections, but also lead to medical device-associated infections. Ever increasing antibiotic resistance due to biofilms has sparked a search for plant-based replacements. This study examines anti-biofilm potential of essential oils from lemon (Citrus limon), lemongrass (Cymbopogon citratus), and lavender (Lavandula officinalis) against 26 clinical isolates of E. coli. Essential oils (EOs) were extracted by using hydro-distillation and characterized by GC-MS and FT-IR. Biofilms were quantitatively and qualitatively evaluated. The extracted essential oils had yields ranging from 1.37 to 1.45 (w/v). The main constituents were limonene (94%) in lemon, citral (42%) and ketones (9%) in lemongrass, and linalool (35%) and linalyl anthranilate (20%) in lavender EO. MICs and MBCs of EOs were in the range of 3.13-50 µg/ml. The oils under investigation exhibited notable biofilm inhibition and eradication properties. The percent inhibition and eradication varied between 6-89% and 3-82.25% (p < 0.0001), respectively. These findings suggest that essential oils from lemon, lemongrass, and lavender may offer a promising natural approach to combat E. coli biofilms and associated infections. Further research is warranted to elucidate the underlying mechanisms of action and to explore their clinical applications.</p>","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biologia futuraPub Date : 2025-03-01Epub Date: 2024-11-27DOI: 10.1007/s42977-024-00246-y
Haniya Mazhar, Ali Afzal, Hanan Afzal, Amara Noureen, Muhammad Mubashir Iqbal Ahmad, Suneela Amaan, Naaz Abbas, Hongxin Zhu, Muhammad Babar Khawar
{"title":"Extracellular lipase production from Bacillus cereus by using agro-industrial waste.","authors":"Haniya Mazhar, Ali Afzal, Hanan Afzal, Amara Noureen, Muhammad Mubashir Iqbal Ahmad, Suneela Amaan, Naaz Abbas, Hongxin Zhu, Muhammad Babar Khawar","doi":"10.1007/s42977-024-00246-y","DOIUrl":"10.1007/s42977-024-00246-y","url":null,"abstract":"<p><p>Lipases are crucial biocatalysts in various industrial applications, and there is considerable interest in developing sustainable methods for their synthesis. This study focuses on the isolation, screening, and comparison of Bacillus cereus strains to produce extracellular lipases utilizing agro-industrial waste through solid-state fermentation. The results indicate that B. cereus exhibited optimal lipase production with soybean extract, yielding 41.2 ± 1.08 µ/ml (p < 0.05), followed by bagasse with 40.5 ± 0.97 µ/ml (p < 0.05). Other substrates, including rice bran (9.9 µ/ml), wheat bran (25.8 µ/ml), sunflower seed (24.0 µ/ml), and oat bran (10.2 µ/ml), demonstrated significantly lower enzyme activity. Additionally, lipase production from fruit peels was assessed, with banana yielding 21.1 µ/ml, orange 20.3 µ/ml, melon 16.3 µ/ml, and watermelon 16.43 µ/ml. Various oil wastes were also evaluated, showing lipase activities of 14.6 µ/ml (Sitara oil), 13.3 µ/ml (Shan oil), 11.0 µ/ml (automobile oil), and 10.2 µ/ml (cooking oil). The bacterial lipases produced from B. cereus demonstrated maximum hydrolysis of tributyrin agar medium at 40°C (p < 0.05). The findings suggest that utilizing different agro-industrial wastes for the production of extracellular lipase could help mitigate environmental pollution while providing a viable option for commercial enzyme production.</p>","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"41-48"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of voluntary or involuntary exercise in adolescent male rats exposed to chronic social isolation on cognition, behavior, and neurotrophic factors.","authors":"Ayca Arslankiran, Burcu Acikgoz, Helin Demirtas, Bahar Dalkiran, Amac Kiray, Ilkay Aksu, Muge Kiray, Ayfer Dayi","doi":"10.1007/s42977-025-00250-w","DOIUrl":"10.1007/s42977-025-00250-w","url":null,"abstract":"<p><p>This study investigated the effects of voluntary and involuntary/regular exercise on neurotrophic factors in the brain, cognitive functions, and anxiety in socially isolated adolescent male rats. In this study, 42 adolescent male Sprague-Dawley rats were divided into six groups: control (C), socially isolated (SI), voluntary exercise (VE), regular exercise (RE), socially isolated + voluntary exercise (SI-VE), and socially isolated + regular exercise (SI-RE). Socially isolated groups were kept in separate cages for 4 weeks. Treadmill and wheel running were used in the exercise groups. The following behavioral tests-elevated plus maze (EPM), open field, ultrasonic vocalization (USV), and Morris water maze (MWM)-rats were euthanized, and brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), and vascular endothelial growth factor (VEGF) levels were measured in the prefrontal cortex (PFC) and hippocampus. Statistically, the differences between the groups were evaluated by one-way ANOVA and post hoc LSD tests using IBM SPSS software. In the EPM, locomotor activity was higher in the voluntary exercise groups. In the MWM, both regular exercise groups found the platform faster. In the USV, the SI-RE group produced more 50-kHz sounds. BDNF and NGF levels in the hippocampus were higher in the SI-RE and SI-VE groups; VEGF levels were higher in the SI-RE group. Neuron density in the PFC increased in the SI-RE and VE groups, while neuron density in the hippocampus increased in the SI-RE, SI-VE, and VE groups. According to the findings, we showed that voluntary exercise reduces social isolation-induced anxiety, and involuntary/regular exercise both reduces anxiety and has potential benefits on cognitive functions.</p>","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"71-85"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biologia futuraPub Date : 2025-03-01Epub Date: 2024-05-07DOI: 10.1007/s42977-024-00222-6
Claudia Fugazza, Fumi Higaki
{"title":"Exploring the use of projected videos to test action matching from different perspectives in dogs.","authors":"Claudia Fugazza, Fumi Higaki","doi":"10.1007/s42977-024-00222-6","DOIUrl":"10.1007/s42977-024-00222-6","url":null,"abstract":"<p><p>Dogs trained with the Do as I Do method can imitate human actions upon request, but their ability to match actions observed from different perspectives remains unknown. The use of 2D video stimuli may enable researchers to systematically manipulate the perspective from which demonstrations are observed, thereby widening the range of methods available to study cognitive skills related to imitation. In this study, we explore the possibility of using 2D stimuli to test action matching in dogs, including when demonstrations are seen from different perspectives. We examined two dogs' imitative performance using videos projected on a screen; while, the owner interacted with the dog remotely through an online meeting software. The dogs were first trained to match human actions seen on a screen frontally, and then were tested when the projected demonstrations were seen frontally, from the side, and from above. Results revealed that both dogs matched the demonstrated actions from frontal and, notably, also from side perspectives, at least to some extent, consistent with familiarity of their daily interactions with humans. However, action matching from an above perspective presented challenges, indicating the potential influence of observational experience and highlighting the importance of perspective manipulation when investigating imitation abilities. These findings show that it is possible to use 2D videos to test imitation in dogs, thereby expanding the potential methodologies to study imitation and other related cognitive skills.</p>","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"33-40"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140875736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The effects of some chemical compounds on the sound absorbing ability of tree bark.","authors":"Serhun Sağlam, Emrah Özdemir, Öznur Özden, Ender Makineci, Ulaş Yunus Özkan, Doğukan Bingöl, Feyza Güzelçimen","doi":"10.1007/s42977-024-00248-w","DOIUrl":"10.1007/s42977-024-00248-w","url":null,"abstract":"<p><p>Tree bark is an important natural polymer for sound absorption. The main components in the bark of different tree species are polymers with high molecular weight such as cellulose, hemicellulose, and lignin. The aim of this study is to determine the noise reduction coefficient (NRC), lignin, alcohol-benzene solubility (ABS), carbon (C), and nitrogen (N) contents in samples taken from the bark of different tree species-black locust (Robinia pseudoacacia), narrow-leaved ash (Fraxinus angustifolia), stone pine (Pinus pinea), silver lime (Tilia tomentosa), sweet chestnut (Castanea sativa), sessile oak (Quercus petraea), and maritime pine (Pinus pinaster) and to investigate the relationship between these chemical properties and sound absorption measurements. Tree species showed a statistically significant difference in terms of all measured variables. In the correlation matrix obtained as a result of the analysis, only ABS showed a significant and the highest positive correlation with the NRC, with a correlation coefficient of r = 0.812. ABS in bark is seen as the most important chemical factor regarding sound retention, indicating the abundance of extractives in barks of different tree species. An investigation into the relationship between sound retention and different extractive substance and contents of different extractive substances in bark is recommended for further studies.</p>","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"49-57"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biologia futuraPub Date : 2025-03-01Epub Date: 2025-02-27DOI: 10.1007/s42977-025-00249-3
Anilkumar Sheela Anusha, Hrishikesan Sujitha Rakendu, Sneha Asha, T S Xavier, Praveen Kumar
{"title":"Biosynthesis of nickel nanoparticles using Enterobacter cloacae: characterization and multifaceted applications.","authors":"Anilkumar Sheela Anusha, Hrishikesan Sujitha Rakendu, Sneha Asha, T S Xavier, Praveen Kumar","doi":"10.1007/s42977-025-00249-3","DOIUrl":"10.1007/s42977-025-00249-3","url":null,"abstract":"<p><p>This study describes the biosynthesis of nickel nanoparticles (Ni NPs) using gram-negative bacterium, Enterobacter cloacae. The synthesized Ni NPs were annealed at 350 °C for 3 h and characterized using various techniques. Powder X-ray diffraction analysis revealed the formation of Ni NPs with an average crystallite size of 48.78 nm. UV-Vis spectroscopy exhibited an absorption peak at 394 nm, while FTIR confirmed a peak at 416 nm. Field emission scanning electron microscopy showed the Ni NPs as a mixture uniform distribution, characterized by almost uniform spherical or hexagonal shapes. The Ni NPs demonstrated moderate antibacterial activity but exhibited notably high biofilm activity against Pseudomonas aeruginosa species, suggesting their potential in combating biofilm-associated infections. Additionally, the biosynthesized Ni NPs showed promising larvicidal and Insecticidal activities. This study provides an eco-friendly and cost-effective method for synthesizing Ni NPs and highlights their multifaceted applications in healthcare and pest control.</p>","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"59-70"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biologia futuraPub Date : 2025-03-01Epub Date: 2025-03-21DOI: 10.1007/s42977-025-00253-7
Ökkeş Atici, İhsan Aydın, Sinem Karakus, Deniz Tiryaki
{"title":"Inoculating maize (Zea mays L.) seeds with halotolerant rhizobacteria from wild halophytes improves physiological and biochemical responses of seedlings to salt stress.","authors":"Ökkeş Atici, İhsan Aydın, Sinem Karakus, Deniz Tiryaki","doi":"10.1007/s42977-025-00253-7","DOIUrl":"10.1007/s42977-025-00253-7","url":null,"abstract":"<p><p>Salinity stress is a major environmental factor that poses a significant constraint to plant growth, threatening agricultural productivity and sustainability. This study investigated the potential of halotolerant bacteria, isolated from the rhizosphere of wild halotolerant plants in Turkey's Salt Lake basin, to enhance salt stress tolerance in maize (Zea mays L.). The rhizospheres of 17 different wild halotolerant plants were selected for bacterial isolation, resulting in the identification of 22 halotolerant bacteria using 16S rRNA sequence analysis. Among these, 19 isolates were found to possess positive activity for 1-aminocyclopropane-1-carboxylate (ACC) deaminase and nitrogen fixation. When the maize seeds inoculated with these 19 isolates were grown under normal conditions, four isolates‒TG-4 (Halomonas arcis), TG-8 (Marinococcus tarigensis), TG-12 (Halobacillus dabanensis), and TG-20 (Halomonas eurihalina)-significantly stimulated seedling growth and development. To evaluate the effect of these four isolates on salt tolerance, inoculated seeds were grown under various salt conditions (0.0, 75, 150, and 250 mM NaCl). The responses of plants to salt stress were analyzed by evaluating growth parameters, membrane damage, photosynthetic pigment and proline content, reactive oxygen species and lipid peroxidation levels, and antioxidant enzyme activities. According to the parameters, the results indicated that TG-4, TG-8, and TG-12, in particular, have the potential to function as plant growth-promoting rhizobacteria and effectively enhance salt stress tolerance in the maize seedlings. Overall, this research highlights the potential of halotolerant bacteria to improve salt stress tolerance in maize plants through multifaceted mechanisms, offering valuable insights for sustainable agriculture in saline environments.</p>","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"101-116"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biologia futuraPub Date : 2025-03-01Epub Date: 2025-04-12DOI: 10.1007/s42977-025-00251-9
Éva Kolics, Zoltán Ács, Tamás Sipos, László Rózsa, János Taller, Kinga Mátyás, Balázs Kolics
{"title":"The first appearance of Vespa velutina nigrithorax in Hungary supports its origin from the established European population.","authors":"Éva Kolics, Zoltán Ács, Tamás Sipos, László Rózsa, János Taller, Kinga Mátyás, Balázs Kolics","doi":"10.1007/s42977-025-00251-9","DOIUrl":"10.1007/s42977-025-00251-9","url":null,"abstract":"<p><p>This study investigates specimens of the Asian yellow-legged hornet (Vespa velutina nigrithorax du Buysson, 1905) originating from the first colony detected in Hungary. Phylogenetic analysis based on the mitochondrial cytochrome oxidase c subunit 1 gene confirms their European origin and definitely identifies them as V. velutina. The detection in Hungary represented the species' farthest documented occurrence from its established European distribution at that time, highlighting its significant dispersal potential. Given the high density of bee populations in the region, particular attention should be paid to control efforts during the early stages of colonization.</p>","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":"76 1","pages":"87-90"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biologia futuraPub Date : 2025-03-01Epub Date: 2024-09-20DOI: 10.1007/s42977-024-00245-z
Muhammad Waqas Mazhar, Muhammad Ishtiaq, Mehwish Maqbool, Eman A Mahmoud, Fahed A Almana, Hosam O Elansary
{"title":"Exploring the potential of plant astrobiology: adapting flora for extra-terrestrial habitats: a review.","authors":"Muhammad Waqas Mazhar, Muhammad Ishtiaq, Mehwish Maqbool, Eman A Mahmoud, Fahed A Almana, Hosam O Elansary","doi":"10.1007/s42977-024-00245-z","DOIUrl":"10.1007/s42977-024-00245-z","url":null,"abstract":"<p><p>In recent years, the realm of astrobiology has expanded beyond the search for microbial life to encompass the intriguing possibility of plant life beyond our planet. Plant astrobiology delves into the adaptations and mechanisms that might allow Earth's flora to flourish in the harsh conditions of outer space and other celestial bodies. This review aims to shed light on the captivating field of plant astrobiology, its implications, and the challenges and opportunities it presents. Plant astrobiology marries the disciplines of botany and astrobiology, challenging us to envision the growth of plants beyond Earth's atmosphere. Researchers in this field are not only exploring the potential for plant life on other planets and moons but also investigating how plants could be harnessed to sustain life during extended space missions. The review discusses how plants could adapt to environments characterized by low gravity, high radiation, extreme temperature fluctuations, and different atmospheric compositions. It highlights the physiological changes necessary for plants to survive and reproduce in these conditions. A pivotal concept is the integration of plants into closed-loop life support systems, where plants would play a crucial role in recycling waste products, generating oxygen, and producing food. The review delves into ongoing research involving genetic modifications and synthetic biology techniques to enhance plants' resilience in space environments. It addresses ethical considerations associated with altering organisms for off-planet habitation. Additionally, the review contemplates the psychological and emotional benefits of having greenery in enclosed, isolated space habitats. The review concludes that by employing advanced research methodologies, the field of plant astrobiology can greatly enhance the viability and sustainability of future space missions, highlighting the essential role of plants in sustaining long-term human presence beyond Earth.</p>","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"1-18"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142280024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Silicon uptake and transport mechanisms in plants: processes, applications and challenges in sustainable plant management.","authors":"Raghvendra Pandey, Chandan Singh, Smita Mishra, Mukhtar Iderawumi Abdulraheem, Deepak Vyas","doi":"10.1007/s42977-024-00247-x","DOIUrl":"10.1007/s42977-024-00247-x","url":null,"abstract":"<p><p>Silicon (Si) is an abundant element in the earth's crust essential for plant growth and development. Recent studies silicon's potential for improving plant resilience to numerous biotic stressors, notably fungal diseases. This review seeks to offer a comprehensive understanding of the processes and advantages of silicon-induced systemic resistance in plants, with a special focus on its interactions with fungal pathogens. Furthermore, we investigate the effect of silicon on plant physiological and biochemical changes, such as enhanced lignification, strengthening of physical barriers, and activation of antioxidant systems. Additionally, we examine the influence of silicon on microbial populations within the rhizosphere and its effects on mycorrhizal associations. Lastly, we discuss the potential applications and challenges of integrating silicon-based strategies in sustainable plant disease management. This review provides valuable insights into using silicon as a novel approach to enhance plant systemic resistance against fungal pathogens, offering prospects for developing eco-friendly and efficient agricultural practices.</p>","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"19-31"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}