Biochimica et biophysica acta. Biomembranes最新文献_第2页

Phase-separated cationic giant unilamellar vesicles as templates for the polymerization of tetraethyl orthosilicate (TEOS) 相分离阳离子巨型单层囊泡作为正硅酸四乙酯（TEOS）聚合模板的研究。

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-02-01 DOI: 10.1016/j.bbamem.2024.184403

Kohei Shiomi , Keita Hayashi , Haruyuki Ishii , Toshiyuki Kamei , Toshinori Shimanouchi , Hidemi Nakamura , Sosaku Ichikawa

{"title":"Phase-separated cationic giant unilamellar vesicles as templates for the polymerization of tetraethyl orthosilicate (TEOS)","authors":"Kohei Shiomi , Keita Hayashi , Haruyuki Ishii , Toshiyuki Kamei , Toshinori Shimanouchi , Hidemi Nakamura , Sosaku Ichikawa","doi":"10.1016/j.bbamem.2024.184403","DOIUrl":"10.1016/j.bbamem.2024.184403","url":null,"abstract":"<div><div>Unlike homogeneous liposomes, phase-separated liposomes have the potential to be attractive soft materials because they exhibit different properties for each phase. In this study, phase separation was observed when liposomes were prepared using 1,2-dioleoyloxy-3-trimethylammonium propane chloride (DOTAP), 1,2-distearoyl-<em>sn</em>-glycero-3-phosphocholine (DSPC), and cholesterol. The pH of the DOTAP-rich phase was evaluated using a coumarin derivative, and measurements showed that DOTAP molecules accumulated hydroxyl ions (OH<sup>−</sup>) in the DOTAP-rich phase. Such accumulation of OH<sup>−</sup> was not observed when homogeneous DSPC liposomes were used. The difference in local concentration of OH<sup>−</sup> in each phase was applied to prepare hollow silica particles with large pores. The OH<sup>−</sup> promotes the polymerization of tetraethyl orthosilicate (TEOS). Therefore, TEOS polymerized preferentially in the DOTAP-rich phase, whereas a silica membrane barely formed in the DSPC-rich phase.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 2","pages":"Article 184403"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simulation analysis of the effect of a cholesterol-dependent fusogenic peptide from HIV gp41 on membrane phospholipid dynamics

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-02-01 DOI: 10.1016/j.bbamem.2025.184413

Manami Nishizawa , Kazuhisa Nishizawa

{"title":"A simulation analysis of the effect of a cholesterol-dependent fusogenic peptide from HIV gp41 on membrane phospholipid dynamics","authors":"Manami Nishizawa , Kazuhisa Nishizawa","doi":"10.1016/j.bbamem.2025.184413","DOIUrl":"10.1016/j.bbamem.2025.184413","url":null,"abstract":"<div><div>CpreTM is a fusogenic peptide whose N-terminal portion is derived from the membrane-proximal external region (MPER) and C-terminal portion covers the transmembrane (TM) domain of gp41 of HIV. CpreTM has been shown to induce membrane fusion, which requires cholesterol molecules as membrane components. To gain insight into the effects of CpreTM on membrane lipid dynamics, we performed molecular dynamics simulations. In conventional simulations, several cholesterol-binding sites were found under the segment derived from MPER and near the cholesterol recognition/interaction amino acid consensus (CRAC) motif located at the C-terminus of MPER. CpreTM resides in shallower positions in the POPC (palmitoyl oleoyl phosphatidylcholine)/cholesterol bilayer than in the pure POPC bilayer. Our metadynamic simulations using the position of one POPC molecule (“target POPC”) as the collective variable showed that CpreTM remarkably lowered the free energy cost for the POPC protrusion from the cholesterol-containing membrane; e.g., the cost for 0.7 nm outward displacement from the height of bulk POPC molecules was decreased by ~10 kJ/mol compared to the peptide-free corresponding system. Such stabilization of the POPC protrusion was not observed in the cholesterol-free POPC membrane. It was more pronounced near the aromatic residues, including the three Trp residues of CpreTM, suggesting important roles for aromatic residues in stabilizing the POPC protrusion.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 3","pages":"Article 184413"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a sorting motif for Tspan3 to MHCII compartments in human B cells 人B细胞中Tspan3到MHCII区室的分选基序的鉴定。

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-02-01 DOI: 10.1016/j.bbamem.2024.184406

Fabian Schwerdtfeger , Martin ter Beest , Cesar A. Perez-Martinez , Kris Raaijmakers , Philipp Michael Hagemann , Aina Martí Juan , Cornelia G. Spruijt , Michiel Vermeulen , Sjoerd van Deventer , Annemiek B. van Spriel

{"title":"Identification of a sorting motif for Tspan3 to MHCII compartments in human B cells","authors":"Fabian Schwerdtfeger , Martin ter Beest , Cesar A. Perez-Martinez , Kris Raaijmakers , Philipp Michael Hagemann , Aina Martí Juan , Cornelia G. Spruijt , Michiel Vermeulen , Sjoerd van Deventer , Annemiek B. van Spriel","doi":"10.1016/j.bbamem.2024.184406","DOIUrl":"10.1016/j.bbamem.2024.184406","url":null,"abstract":"<div><div>Tetraspanins are four-transmembrane proteins that play fundamental roles in the immune system by enabling processes like migration, proliferation, signaling and protein trafficking. While the importance of cell surface tetraspanins has been established, the function of intracellular tetraspanins is less well understood. Here, we investigated the role of tetraspanin 3 (Tspan3) in lymphocytes. Tspan3 expression was low in T cells and high in B cells which increased during B cell activation. Tspan3 localized to late endosomal structures where it colocalized with major histocompatibility complex II (MHCII) in the MHCII compartment. There, inhibiting the formation of intraluminal vesicles (ILVs) showed that Tspan3 localization was not affected in contrast to its homologue CD63. Using a peptide-pulldown approach, we identified that Tspan3 interacts with AP2 via its C-terminus that harbors a YXXΦ-based sorting motif. Interestingly, mutating this motif did not impair Tspan3 localization. Instead, leucine 246 was required for its intracellular localization, identifying an unrecognized leucine-based sorting motif responsible for Tspan3 localization to MHCII compartment in B cells. Taken together, we report a new sorting motif for Tspan3 to MHCII compartments in human B cells.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 2","pages":"Article 184406"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural modification of lipid membranes by polyphenols: A fluorescence spectroscopy study

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-01-22 DOI: 10.1016/j.bbamem.2025.184411

Valeriya M. Trusova , Uliana K. Tarabara , Mette H. Thomsen , Galyna P. Gorbenko

{"title":"Structural modification of lipid membranes by polyphenols: A fluorescence spectroscopy study","authors":"Valeriya M. Trusova , Uliana K. Tarabara , Mette H. Thomsen , Galyna P. Gorbenko","doi":"10.1016/j.bbamem.2025.184411","DOIUrl":"10.1016/j.bbamem.2025.184411","url":null,"abstract":"<div><div>The present study investigates the molecular mechanisms of polyphenol-lipid interactions and their impact on membrane properties. Using pyrene and DPH as reporter molecules, we examined the impact of quercetin, curcumin, gallic, and salicylic acids on membranes composed of phosphatidylcholine (PC) and its mixtures with phosphatidylglycerol (PG), cardiolipin (CL), and cholesterol (Chol). Quercetin was found to increase the lipid order without affecting the lipid bilayer free volume, indicating interactions near the membrane surface. In turn, curcumin exhibited more complex effects, reducing free volume in PC but increasing it in PG vesicles, reflecting its amphiphilic structure and variable penetration depth. Gallic and salicylic acids selectively increased free volume at the membrane core without influencing lipid order at the upper regions of lipid bilayer. The results obtained demonstrate that polyphenol structure and lipid composition dictate the resultant pattern of polyphenol-membranes interactions, which may have implications for drug delivery and nutraceutical design.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 3","pages":"Article 184411"},"PeriodicalIF":2.8,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human cathelicidin LL-37 rapidly disrupted colonic epithelial integrity 人cathelicidin LL-37迅速破坏结肠上皮的完整性。

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-01-19 DOI: 10.1016/j.bbamem.2025.184410

Geeta Kilari, Jacquelyn Tran, Graham A.D. Blyth, Eduardo R. Cobo

{"title":"Human cathelicidin LL-37 rapidly disrupted colonic epithelial integrity","authors":"Geeta Kilari, Jacquelyn Tran, Graham A.D. Blyth, Eduardo R. Cobo","doi":"10.1016/j.bbamem.2025.184410","DOIUrl":"10.1016/j.bbamem.2025.184410","url":null,"abstract":"<div><div>The intestinal barrier, held together by epithelial cells and intercellular tight junction (TJ) proteins, prevents the penetration of microbial pathogens. Concurrently, intestinal epithelial cells secrete antimicrobial peptides, including cathelicidin. Cathelicidin has direct antibacterial and immunomodulatory functions, although its role in intestinal integrity remains elusive. In this study, we demonstrate that direct stimulation of human colonic epithelial (T84) cells with human cathelicidin, LL-37, resulted in a rapid and transient increase in epithelial cell permeability. This increased permeability was associated with the TJ proteins occludin and claudin-2 degradation, mediated by these specific proteins' endocytosis and lysosomal degradation. While murine cathelicidin (CRAMP) failed to modify T84 cell permeability, LL-37 degraded TJ proteins in murine rectal epithelial (CMT-93) cells. The stimulus of (CMT-93) cells with LL-37 aggravated the cell permeability and furthered TJ degradation provoked by the intestinal pathogen, attaching/effacing (A/E) <em>Citrobacter rodentium</em> (<em>C. rodentium</em>). The number of <em>C. rodentium</em> that colonized CMT-93 cells was not severely impacted by the presence of LL-37. While a temporary disruption of tight junctions by LL-37 may lead to a ‘leaky gut,’ this study demonstrates that LL-37 increases epithelial cell permeability by degrading TJ proteins occludin and claudin-2 through endocytosis and lysosomal degradation. These immunomodulatory actions occurring at concentrations lower than those microbicidal uncover a new guise for cathelicidin modulating the epithelial barrier against A/E pathogens. Recognizing native cathelicidin's functions in a specified disease setting (e.g., colitis) will help establish it as an anti-infectious immunomodulator.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 3","pages":"Article 184410"},"PeriodicalIF":2.8,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential effects of endo- and exopolyphosphatase expression on the induction of the mitochondrial permeability transition pore 内多磷酸酶和外多磷酸酶表达对线粒体通透性过渡孔诱导的差异影响。

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-01-07 DOI: 10.1016/j.bbamem.2025.184408

Yaw Akosah , Vedangi Hambardikar , Maria Neginskaya , Sally Morris , Maria E. Solesio , Evgeny V. Pavlov

{"title":"Differential effects of endo- and exopolyphosphatase expression on the induction of the mitochondrial permeability transition pore","authors":"Yaw Akosah , Vedangi Hambardikar , Maria Neginskaya , Sally Morris , Maria E. Solesio , Evgeny V. Pavlov","doi":"10.1016/j.bbamem.2025.184408","DOIUrl":"10.1016/j.bbamem.2025.184408","url":null,"abstract":"<div><div>Inorganic polyphosphate (polyP) is a polymer that consists of a series of orthophosphates connected by high-energy phosphoanhydride bonds, like those found in ATP. In mammalian mitochondria, polyP has been linked to the activation of the mitochondrial permeability transition pore (mPTP). However, the details of this process are not completely understood. The activation of mPTP by polyP may involve the regulation of bioenergetics, Ca<sup>2+</sup> buffering, or direct involvement in mPTP channel formation. In this study, using refractive index imaging techniques, we examined mPTP induction in both wild-type (WT) SH-SY5Y cells, and mutant SH-SY5Y cells expressing either mitochondrially targeted exopolyphosphatase (MitoPPX), which depletes polyP by breaking free terminal phosphoanhydride bonds; or endopolyphosphatase (MitoPPN), which cleaves internal phosphoanhydride bonds and thus can target polyP pool with protected terminal groups. Upon treating the cells with the calcium ionophore ferutinin, the influx of Ca<sup>2+</sup> triggered mitochondrial membrane depolarization and permeabilization in both WT and MitoPPX cells indicating activation of mPTP. However, in MitoPPN cells, mitochondrial depolarization occurred without mPTP activation. Based on these findings we propose the possibility that activation of mPTP is not linked to the pool of free polyP. This supports the hypothesis that polyP is either an important structural component of the mPTP channel or associated with other macromolecular complexes involved in mPTP induction.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 3","pages":"Article 184408"},"PeriodicalIF":2.8,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidating the structure and function of a membrane-active plant protein domain using in silico mutagenesis 阐明膜活性植物蛋白结构域在硅诱变中的结构和功能。

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-01-07 DOI: 10.1016/j.bbamem.2025.184409

Lennie K.Y. Cheung , Sebastian Thallmair , Rickey Y. Yada

{"title":"Elucidating the structure and function of a membrane-active plant protein domain using in silico mutagenesis","authors":"Lennie K.Y. Cheung , Sebastian Thallmair , Rickey Y. Yada","doi":"10.1016/j.bbamem.2025.184409","DOIUrl":"10.1016/j.bbamem.2025.184409","url":null,"abstract":"<div><div>The <em>Solanum tuberosum</em> (common potato) plant specific insert (<em>St</em>PSI) is an antimicrobial protein domain that exhibits membrane-disrupting and membrane-fusing activity upon dimerization at acidic pH, activity proposed to involve electrostatic attraction and membrane anchoring mediated by specific positively-charged and conserved tryptophan residues, respectively. This study is the first to employ an in silico mutagenesis approach to clarify the structure-function relationship of a plant specific insert (PSI), where ten rationally-mutated <em>St</em>PSI variants were investigated using all-atom and coarse-grained molecular dynamics. The tryptophan (W) residue at position 18 (W18) of wild-type <em>St</em>PSI was predicted to confer structural flexibility to the dimer and mediate a partial separation of the assembled monomers upon bilayer contact, while residues including W77 and the lysine (K) residue at position 83 (K83) were predicted to stabilize secondary structure and influence association with the model membrane. Mechanisms predicted to influence <em>St</em>PSI-membrane association included the partial separation of assembled monomers on the bilayer surface, formation of a specific salt bridge, and membrane anchoring of hinge 2 residues. The findings suggested that the structure-function relationship of <em>St</em>PSI involved several mechanisms that may each be modulated by specific key residues, insights that may support efforts to develop PSI with tailored membrane association for novel applications in plant biotechnology and crop protection.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 3","pages":"Article 184409"},"PeriodicalIF":2.8,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving the antimicrobial potential of the peptide CIDEM-501 through acylation: A computational approach 通过酰化提高肽CIDEM-501的抗菌潜力：一种计算方法。

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-01-07 DOI: 10.1016/j.bbamem.2025.184407

Daniel Alpízar-Pedraza , Adrian Romero-Rivero , Rolando Perdomo-Morales , Niurys Mantilla-García , Claudia Pérez-Martínez , Hilda Garay-Pérez , Frank Rosenau , Ludger Ständker , Vivian Montero-Alejo

{"title":"Improving the antimicrobial potential of the peptide CIDEM-501 through acylation: A computational approach","authors":"Daniel Alpízar-Pedraza , Adrian Romero-Rivero , Rolando Perdomo-Morales , Niurys Mantilla-García , Claudia Pérez-Martínez , Hilda Garay-Pérez , Frank Rosenau , Ludger Ständker , Vivian Montero-Alejo","doi":"10.1016/j.bbamem.2025.184407","DOIUrl":"10.1016/j.bbamem.2025.184407","url":null,"abstract":"<div><div>Acylation is a common method used to modify antimicrobial peptides to enhance their effectiveness. It increases the interactions between the peptide and the bacterial cell membranes. However, acylation can also reduce the selectivity of the peptides by making them more active on eukaryotic membranes, which can lead to unintended toxicity. This study examines the potential of using <em>in silico</em> tools to evaluate the interaction and selectivity of the antimicrobial peptide CIDEM-501 when acylated with decanoic acid at the N-terminus, compared to the non-acylated counterpart. Circular dichroism, microdilution, and hemolysis assays were used to determine the peptide's secondary structure, antimicrobial activity, and selectivity to validate the theoretical predictions. The acylated peptide showed a more stable interaction with the bacterial membrane by inserting the acyl chain into the membrane's hydrophobic core, which led to tighter adsorption and a greater buried surface area. Additionally, it significantly altered membrane order more than the non-acylated counterpart, suggesting superior antimicrobial potential. Finally, <em>in vitro</em> activity assays confirmed theoretical predictions, showing that the acylated peptide had lower Minimum Inhibitory Concentration (MIC) values than the non-acylated peptide. Neither peptide showed significant hemolytic activity at their MIC. The computational techniques used in this study displayed strong predictive capability and helped to elucidate the interaction between the peptide and the membranes.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 3","pages":"Article 184407"},"PeriodicalIF":2.8,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential insertion of arginine in saturated and unsaturated lipid vesicles 精氨酸在饱和和不饱和脂质囊泡中的不同插入。

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2024-12-18 DOI: 10.1016/j.bbamem.2024.184405

Hugo A. Perez, María A. Brandan, Aníbal Disalvo, María de los A. Frías

{"title":"Differential insertion of arginine in saturated and unsaturated lipid vesicles","authors":"Hugo A. Perez, María A. Brandan, Aníbal Disalvo, María de los A. Frías","doi":"10.1016/j.bbamem.2024.184405","DOIUrl":"10.1016/j.bbamem.2024.184405","url":null,"abstract":"<div><div>In this work, the effects of L- Arginine (L-Arg) insertion on saturated and unsaturated lipid membranes were assessed by fluorescence spectroscopy, dynamic light scattering (DLS) and monolayer measurements. The studied systems were composed by DPPC, 16:0 DietherPC, 16:1 Δ9-CisPC, DPPC:Chol, 16:1 Δ9-CisPC:Chol, and 16:1 Δ9-CisPC:DPPC in the presence of increasing concentrations of L-Arg.</div><div>The information obtained using fluorescence spectral Laurdan properties indicates that L- Arg produces a decrease in the polarizability of saturated lipids congruent with the increase in vesicle size and area per lipid. However, in unsaturated lipids, the polarizability increases without significant changes in size and area per lipid denoting a different mechanism of insertion. The two opposite effects can be modulated by the saturated and unsaturated ratio and are independent of carbonyl groups. This modulation is damped by cholesterol. The differences in the L-Arg insertion can be explained considering that in the presence of the double bonds, L-Arg decreases the organized water in the lipid matrix without expanding the bilayer. Instead, in saturated lipid membranes, L-Arg inserts into the acyl chains dragging water and expanding the membrane area.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 3","pages":"Article 184405"},"PeriodicalIF":2.8,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanodomains enriched in arachidonic acid promote P2Y12 receptor oligomerization in the platelet plasma membrane 富含花生四烯酸的纳米域可促进血小板质膜中 P2Y12 受体的寡聚化。

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2024-11-16 DOI: 10.1016/j.bbamem.2024.184402

Florentin Allemand , Semen Yesylevskyy , Jennifer Lagoutte-Renosi , Siamak Davani , Christophe Ramseyer

{"title":"Nanodomains enriched in arachidonic acid promote P2Y12 receptor oligomerization in the platelet plasma membrane","authors":"Florentin Allemand , Semen Yesylevskyy , Jennifer Lagoutte-Renosi , Siamak Davani , Christophe Ramseyer","doi":"10.1016/j.bbamem.2024.184402","DOIUrl":"10.1016/j.bbamem.2024.184402","url":null,"abstract":"<div><div>P2Y12 receptors on the platelet plasma membrane are targeted by several antiplatelets drugs. Although oligomerization and functioning of P2Y12 receptors depend on the membrane environment, little is known about their preferred membrane localization and the role of surrounding lipid composition, especially the arachidonic acids (ARA), which are abundant in platelets. Coarse-grained molecular dynamics simulations of platelet plasma membrane based on the lipidomics data were used to investigate the P2Y12 lipid environment and the involvement of ARA in its oligomerization in platelet plasma membranes. The platelet plasma membrane contains two types of lipids nanodomains: ordered, enriched in SM and cholesterol, and disordered, enriched in ARA-containing lipids. P2Y12 receptors prefer to localize in these ARA-rich domains and induce the sorting of the ARA-containing lipids in their vicinity. This ARA-rich environment promotes the oligomerization of P2Y12 receptors and stabilizes the protein-protein interfaces of oligomers. As summary, oligomerization of P2Y12 receptors is promoted in ARA-rich nano-domains of the platelet plasma membrane.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 1","pages":"Article 184402"},"PeriodicalIF":2.8,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0