Biochimica et biophysica acta. Biomembranes最新文献

{"title":"Modulation of TNAP activity and apatite formation in biomimetic matrix vesicles studied by 31P solid-state NMR","authors":"B.Z. Favarin ,&nbsp;N. Nassif ,&nbsp;T. Azaïs ,&nbsp;J. Guignier ,&nbsp;S. Mebarek ,&nbsp;R. Buchet ,&nbsp;J.L. Millán ,&nbsp;A.P. Ramos ,&nbsp;A.J. Costa-Filho ,&nbsp;P. Ciancaglini","doi":"10.1016/j.bbamem.2025.184446","DOIUrl":"10.1016/j.bbamem.2025.184446","url":null,"abstract":"<div><div>Skeletal and dental mineralization relies on a precisely regulated sequence of events culminating in apatite deposition onto collagen fibrils. Matrix vesicles (MVs), extracellular vesicles released by mineralization-competent cells, play a pivotal role in this process through the catalytic activity of alkaline phosphatase (TNAP). The lipid composition of MVs, particularly phosphatidylserine (PS)-calcium complexes, facilitates the nucleation of amorphous calcium phosphate and apatite formation. However, the interplay between the TNAP structure, the lipid membrane environment, and its enzymatic activity remains incompletely understood.</div><div>Biomimetic models of MVs, as proteoliposomes made with dipalmitoylphosphatidylcholine (DPPC) and various TNAP mutants, were used to investigate the TNAP's activity and mineralization potential. Molecular docking and site-directed mutagenesis revealed that specific cysteine substitutions near TNAP's catalytic and anchoring sites influence structural stability, enzymatic activity, and incorporation into lipid bilayers. Notably, TNAP mutants S221C and P307C exhibited enhanced catalytic efficiency in DPPC liposomes, while A420C showed reduced activity due to steric hindrance near the catalytic site. Solid-state NMR and cryo-TEM analyses confirmed hydroxyapatite formation, with significant contributions from lipid-anchored TNAP to the mineralization process.</div><div>These findings highlight the critical influence of the lipid environment on TNAP's functional properties and provide insights into the mechanisms governing biomineralization and related pathologies, including hypophosphatasia associated with various TNAP mutations. The study underscores the importance of ATP and pyrophosphate hydrolysis by TNAP in modulating apatite formation and reveals the role of specific TNAP mutations in regulating enzymatic activity, stability, and mineral propagation. Understanding these interactions could lead to alternate therapeutic strategies in treatment and regenerative medicine.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 8","pages":"Article 184446"},"PeriodicalIF":2.5,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144940771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complexes of star-shaped block copolymers of poly(2-alkyl-2-oxazine)s and curcumin can affect lipid bilayers mimicking biomembranes","authors":"Svetlana S. Efimova ,&nbsp;Vera A. Martynyuk ,&nbsp;Nina D. Kozina ,&nbsp;Tatyana Y. Kirila ,&nbsp;Alexander P. Filippov ,&nbsp;Olga S. Ostroumova","doi":"10.1016/j.bbamem.2025.184443","DOIUrl":"10.1016/j.bbamem.2025.184443","url":null,"abstract":"<div><div>Poly(2-alkyl-2-oxazine)s (PAlOz) are promising tools for developing delivery systems due to their high biocompatibility, resistance to enzyme hydrolysis, and ability to degrade in biological environments. Here, we investigated the effects of hexaaza[2₆]cyclophane (CPh6), poly(2-methyl-2-oxazine) (PMedOz), a block copolymer of poly(2-ethyl-2-oxazine) (PEtOz) and poly(2-isopropyl-2-oxazine) (PiPrOz), star-shaped block copolymers with six PAlOz arms and a CPh6 branching center (CPh6-PAlOz), and the complexes of all these macromolecules with curcumin on lipid bilayers mimicking the membranes of normal and cancer cells. Curcumin alone demonstrated a pronounced ability to reduce the boundary potential of lipid bilayers composed of phosphatidylcholine or phosphatidylserine, while PMedOz and PEtOz-b-PiPrOz copolymers exhibited either no or weak effects on the electrical properties of biomimetic model membranes. CPh6-PAlOz star-shaped block copolymers were able to interact with K⁺-nonactin. Differential scanning microcalorimetry of the gel-to-liquid crystalline phase transition of membrane lipids indicated that curcumin and all tested macromolecules had more pronounced effects on phosphatidylserine melting than on the phase behavior of phosphatidylcholine. A star-shaped block copolymer with a [PEtOz]/[PiPrOz] ration of 0.8 significantly decreased the melting point of phosphatidylserine. The disordering effects of complexes of curcumin with CPh6, PEtOz-b-PiPrOz copolymers, or the CPh6-PAlOz star-shaped block copolymer with a [PEtOz]/[PiPrOz] ratio of 5 on phosphatidylserine bilayers were less than the algebraic sum of the effects of the polymers and curcumin separately. These data indicate that the carrier cannot be considered an inert matrix that does not affect the biological activity of the transferred active compound, and this should be taken into account when assessing the biological consequences.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 8","pages":"Article 184443"},"PeriodicalIF":2.5,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144895469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the anticancer potential of spider venom peptide Latarcin Ltc2a against triple negative breast cancer","authors":"Prasanjeet Kaur ,&nbsp;Srabaita Roy ,&nbsp;Shilpi Minocha ,&nbsp;Archana Chugh","doi":"10.1016/j.bbamem.2025.184442","DOIUrl":"10.1016/j.bbamem.2025.184442","url":null,"abstract":"<div><div>Cancer remains one of the most formidable challenges to human health, necessitating constant exploration of innovative therapeutic strategies. Among the myriad potential candidates, peptides from venom have emerged as potent sources of bioactive molecules possessing diverse pharmacological properties. In this study, we repurposed a spider venom-derived antimicrobial peptide, Ltc2a, into a selective anticancer agent, bridging microbial defense with cancer therapeutics. Our findings reveal that Ltc2a exhibits selective cytotoxicity towards cancer cells compared to normal cells at just 2 μM of the peptide concentration. Ltc2a induced rapid cytotoxicity within 1 h in breast cancer cells and it was accompanied by membrane disruption as shown by propidium iodide (PI) positive staining and visible damage to cancer cell membranes under field emission scanning electron microscopy (FESEM). <em>In vivo</em> studies using a zebrafish model indicated favorable uptake and a lack of acute toxicity, depicting 80 % survival rate up to 4 μM of tested peptide concentration. Interestingly, the truncated variants of Ltc2a retained their alpha helical structure and demonstrated preferential uptake in MDA-MB-231 cells over HEK293T cells. These findings highlight the therapeutic potential of Ltc2a as selective anticancer peptide with minimal toxicity, paving the way for further preclinical development.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 7","pages":"Article 184442"},"PeriodicalIF":2.5,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144880266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The coronavirus spike HR2 domain: An obscure player entering the limelight during membrane fusion?","authors":"Elena T. Aliper ,&nbsp;Roman G. Efremov","doi":"10.1016/j.bbamem.2025.184445","DOIUrl":"10.1016/j.bbamem.2025.184445","url":null,"abstract":"<div><div>The coronavirus spike protein, the key entity effectuating membrane fusion, cannot exist without membrane-active fragments. In addition to fusion peptides, among such domains are HR1 and HR2. Crucial to the spike's refolding and membrane fusion, they are believed to both interact with each other and bind to the membranes that are merged. To elucidate HR2's precise role in this process, an understanding of its structure and behaviour is required. Here, we used various computational approaches to study SARS-CoV-2 spike HR2's (1163-1211) interaction with membranes in the context within which it operates in live spike. During simulations with model bilayers, HR2 remained hugely unresponsive to the presence of a membrane, however, when extended to include the transmembrane domain (TMD) (1212-1234) and/or membrane-active preHR2 fragment (1147-1161), HR2’s binding to model bilayers was markedly enhanced. The trimeric coiled-coil of HR2 does not dissociate either on its own or with added TMD and/or preHR2. Molecular hydrophobicity potential (MHP) mapping showed that HR2's central part possesses a tilted oblique-oriented motif characteristic of “textbook” membrane-active peptides, albeit flanked by highly hydrophilic fragments. A truncated HR2 only encompassing this motif had a greater affinity for membranes, suggesting HR2 has a modular structure with a membrane-active segment masked by flanking regions and might be potentiated by HR2-adjacent domains and other factors coming into play after the spike gets enzymatically cleaved. Such a modular structure may have evolved for HR2's membrane activity to be regulated very subtly and “switched on” at precisely the right moment during viral fusion.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 8","pages":"Article 184445"},"PeriodicalIF":2.5,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the correlation between proton transfer of phenolic hydroxyl groups of 6 flavonoid compounds and their antioxidant activities","authors":"Shuangmei Gong ,&nbsp;Changying Yang ,&nbsp;Min Li ,&nbsp;Xiaobo Li","doi":"10.1016/j.bbamem.2025.184444","DOIUrl":"10.1016/j.bbamem.2025.184444","url":null,"abstract":"<div><div>This study investigated six flavonoids' photophysical properties in various environments, revealing their pKa variations (e.g., quercetin: 7.364 in water vs 9.329 in vesicles) and demonstrating vesicle/liposome systems' protective effects by slowing proton transfer and oxidation. Metal ion complexation occurred preferentially at specific hydroxyl-keto sites (3-OH-4-keto for quercetin/kaempferol, 5-OH-4-keto for apigenin/baicalein), while structural features like phenolic hydroxyl arrangement and double bonds significantly influenced these interactions. Importantly, the work established that vesicular systems provide superior protection against proton transfer and oxidation compared to liposomes.ANS fluorescence quenching studies further characterized their molecular behaviors, providing key insights into flavonoid stabilization mechanisms and pharmacological activity foundations.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 8","pages":"Article 184444"},"PeriodicalIF":2.5,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144926006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accurate antibiotic accumulation in Enterobacteriaceae isolates expressing efflux pumps","authors":"Jinane Tabcheh ,&nbsp;Julia Vergalli ,&nbsp;Jean-Marie Pagès ,&nbsp;Jean Michel Brunel","doi":"10.1016/j.bbamem.2025.184441","DOIUrl":"10.1016/j.bbamem.2025.184441","url":null,"abstract":"<div><div>In <em>Enterobacteriaceae</em>, antibiotic susceptibility is frequently influenced by mechanisms such as membrane modifications, target site mutations, and enzymatic resistance barriers. Recently, there has been a notable rise in <em>Klebsiella pneumoniae</em>, <em>Escherichia coli</em>, and <em>Enterobacter cloacae</em> isolates exhibiting antibiotic resistance in hospital settings. Of particular concern, some resistant isolates employ membrane-associated resistance mechanisms that significantly lower intracellular antibiotic concentrations, reducing them below the threshold required for therapeutic efficacy. Advancements in methods for quantifying drug accumulation within bacterial cells have provided critical insights into these resistance mechanisms. A key step in these studies relies on cell lysis to release intracellular contents including antibacterial molecules for precise quantification. However, current lysis methods are often time-consuming, underscoring the need for a robust, efficient approach to accurately measure intracellular antibiotic concentrations in isolates exhibiting various levels of efflux pump activity. In this study, we developed a rapid and reliable lysis protocol that minimizes the risk of drug alteration while enabling precise and reproducible measurement of intracellular antibiotic concentrations allowing an evidence-based study of efflux in resistant clinical strains of <em>Enterobacteriaceae</em>. This approach holds significant promise for enhancing our understanding of membrane-associated resistance mechanisms and for informing the optimization of treatment strategies.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 7","pages":"Article 184441"},"PeriodicalIF":2.5,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144809825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rescue of sirtuin inhibitor-dependent decrease in claudin-4 expression and paracellular barrier property in keratinocytes by epigallocatechin gallate","authors":"Maika Miwa ,&nbsp;Miki Tanabe ,&nbsp;Shunsuke Matsuda ,&nbsp;Kento Yamakawa ,&nbsp;Yuta Yoshino ,&nbsp;Norihiro Tada ,&nbsp;Akichika Itoh ,&nbsp;Akira Ikari","doi":"10.1016/j.bbamem.2025.184440","DOIUrl":"10.1016/j.bbamem.2025.184440","url":null,"abstract":"<div><div>The barrier function of granular layer in the skin is mainly sustained by claudin-1 (CLDN1) and CLDN4, tight junctional components. We recently found that the activity of sirtuin-2 (SIRT2), an anti-aging molecule, is decreased with aging in keratinocytes, leading to the attenuation of CLDN4 expression and paracellular barrier function. SIRT2 may be a novel target for enhancing skin barrier function in elderly people. <em>In vitro</em> SIRT2 activity assay showed that epigallocatechin gallate (EGCG) and green tea extract (GT) have a potent ability to activate SIRT2. Tenovin-1 (Ten-1), a sirtuin-1/2 inhibitor, decreased the SIRT2 activity in human keratinocyte-derived HaCaT cells, which was rescued by EGCG and GT. Ten-1 decreased the protein level of CLDN4, which was rescued by EGCG, whereas CLDN1 expression was changed by neither Ten-1 nor EGCG. Ten-1 decreased the tight junctional localization of CLDN4, transepithelial electrical resistance, and paracellular permeability to FD4, a fluorescence paracellular flux marker, which were rescued by EGCG. Ten-1 increased the acetylation level of CLDN4, which was inhibited by EGCG without affecting NAD⁺ content, a substrate for SIRT2. The protein levels of wild-type and K191A mutant were decreased by Ten-1, whereas that of K196A was not. Furthermore, Ten-1 increased the acetylation levels of WT and K191A mutant. We suggest that Ten-1 decreases CLDN4 expression mediated by the acetylation of K196 of CLDN4 and EGCG is useful to protect from aging-induced dysfunction of paracellular barrier in the keratinocytes.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 7","pages":"Article 184440"},"PeriodicalIF":2.5,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144781745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel flavonoid-magnesium complexes as inhibitors of plasma membrane calcium ATPase","authors":"Mallku Q. Ontiveros ,&nbsp;Mariel Marder ,&nbsp;Rolando C. Rossi ,&nbsp;Juan Pablo Rossi ,&nbsp;Irene C. Mangialavori ,&nbsp;Mariela S. Ferreira-Gomes","doi":"10.1016/j.bbamem.2025.184438","DOIUrl":"10.1016/j.bbamem.2025.184438","url":null,"abstract":"<div><div>Plasma membrane calcium ATPases (PMCAs) are essential for regulating intracellular calcium (Ca²⁺) levels by extruding it from the cytosol. Improper regulation of these transporters is associated with numerous diseases, including neurological, cardiovascular, oncological, and metabolic problems, rendering them interesting targets for therapeutic intervention. However, there is a scarcity of specific tools to adjust PMCA activity. Flavonoids, a varied group of polyphenolic compounds with numerous biological effects, have been demonstrated to affect the function of several ATPases, including PMCAs.</div><div>In this study, we investigated the inhibitory mechanism of quercetin on the human PMCA4 isoform (hPMCA4). Using UV–visible spectroscopy and ATPase activity assay, we identified a high-affinity inhibition mediated by a quercetin‑magnesium (Mg²⁺) complex with a Ki of 49.7 ± 1.5 nM. Functional and phosphorylation studies at different pHs suggest that quercetin affects PMCA activity through two inhibitory mechanisms: a high-affinity one mediated by the quercetin-Mg²⁺ complex and a low-affinity one mediated by the free flavonoid.</div><div>Analysis of the structure-activity relationship revealed that hydroxyl groups at positions 3′, 4′, and 3 are critical for complex formation and inhibitory potency. Specifically, the 3′ and 4′ hydroxyls are required to form the PMCA inhibitory complex. These findings demonstrate a novel mechanism of PMCA activity modulation involving flavonoid-Mg²⁺ complexes, which emerge as selective molecular tools capable of regulating Ca²⁺ transport. This knowledge provides new insights into designing PMCA inhibitors and exploring therapeutic strategies targeting diseases linked to calcium signalling dysfunction.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 7","pages":"Article 184438"},"PeriodicalIF":2.5,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of biophysical properties of ion channels with nonlinear methods","authors":"Mahmut Akilli ,&nbsp;Fatma Söğüt ,&nbsp;Ülkü Çömelekoğlu ,&nbsp;Handan Tuncel","doi":"10.1016/j.bbamem.2025.184437","DOIUrl":"10.1016/j.bbamem.2025.184437","url":null,"abstract":"<div><div>The aim of this study is to show how nonlinear methods can be used to investigate the biophysical properties of ion channels. For this purpose, the membrane ion current signals of the EAG1 potassium channel of MCF-7 cells and of the TRP channel of ARPE-19 cells were used. Entropy measurements and maximum Lyapunov exponent were chosen as nonlinear methods. The vital state functional of the ion channels in the membrane was monitored using the entropy parameter. The behavioural or functional sensitivity of ion channels was quantified by the maximum Lyapunov exponent. It is known that the entropy of a system increases as it moves towards equilibrium. In this context, during the electrical activity of a living cell, the entropy of the cell reaches its maximum when the membrane ion fluxes reach the equilibrium, that is, when the value of the ion fluxes approaches zero. Therefore, the accuracy of the results obtained in this study was calibrated by reference to this general assumption. The results show functional differences between the MCF-7 EAG1 potassium channel and the ARPE-19 TRP channel. This method has potential applications in analysing cell behaviour or studying ion channel biophysical properties. It can also be used to observe differences in the behaviour of normal and cancerous cells of the same type, or to measure the effects of drugs on the cell.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 7","pages":"Article 184437"},"PeriodicalIF":2.5,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144757922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The phospholipolytically active neurotoxin Vipoxin induces changes of the mechanical properties of breast epithelial cells","authors":"Will Linthicum ,&nbsp;Qi Wen ,&nbsp;Nancy A. Burnham ,&nbsp;Svetla D. Petrova ,&nbsp;Konstantin Balashev","doi":"10.1016/j.bbamem.2025.184434","DOIUrl":"10.1016/j.bbamem.2025.184434","url":null,"abstract":"<div><div>The investigation of how drugs or toxins alter cell mechanics is gaining significant traction in biomedical science, driven by the dual objectives of elucidating their mechanisms of action and enhancing drug screening processes. In this study, Atomic Force Microscopy (AFM), a prominent experimental technique in recent years, was employed to examine and analyze the mechanical responses of cells exposed to the neurotoxin Vipoxin. This method enables the precise measurement of key mechanical parameters such as cell stiffness and viscoelasticity before and after toxin introduction in the cell culture. It was demonstrated that the cells' stiffness and viscosity decreased with increasing Vipoxin concentration. Additionally, Total Internal Reflection Fluorescence Microscopy (TIRFM) was utilized to monitor morphological changes in the cells over time. These morphological changes were quantitatively analyzed using fractal analysis of the acquired images. The observed changes in cell shapes implied the reorganization of the cell cytoskeleton, thus providing insight into a comprehensive understanding of cell mechanics under the influence of Vipoxin.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 7","pages":"Article 184434"},"PeriodicalIF":2.8,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144616113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}