Avian Pathology最新文献_第4页

A dual gC-gD glycoprotein-based indirect ELISA for enhanced detection of anatid herpesvirus 1 antibodies. 基于双gC-gD糖蛋白的间接ELISA强化检测无抗体疱疹病毒1抗体

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-09-11 DOI: 10.1080/03079457.2026.2704779

Shinjini Bhattacharya, Pooja Agarwal, Sachin Kumar

{"title":"A dual gC-gD glycoprotein-based indirect ELISA for enhanced detection of anatid herpesvirus 1 antibodies.","authors":"Shinjini Bhattacharya, Pooja Agarwal, Sachin Kumar","doi":"10.1080/03079457.2026.2704779","DOIUrl":"10.1080/03079457.2026.2704779","url":null,"abstract":"Anatid herpesvirus 1 (AnHV-1) is still a significant limiting factor in the waterfowl industry, and there is a clear demand for field-applicable serodiagnostic assays that are robust, sensitive and reproducible. In this report, we designed and conducted a comprehensive comparative evaluation of whole virus or recombinant glycoprotein C (gC), glycoprotein D (gD) alone or a mixture of gC-gD ELISA, to determine which one had the better diagnostic performance. The recombinant proteins were produced, purified, and optimized for use in indirect ELISA, and subsequent validation was carried out using a set of 120 field serum samples. Receiver operating characteristic (ROC) curve analysis demonstrated that the combined gC-gD ELISA exhibited an almost perfect diagnostic performance (AUC = 0.9925), and high sensitivity (93%) and specificity (95%), surpassing those of two antigen-based assays and the whole virus assay. Although both gC ELISA (high sensitivity 95% but moderate specificity 80%) and gD ELISA (outstanding sensitivity 99%, but lower overall accuracy: AUC = 0.9150) performed well, the sensitivity of the whole virus ELISA was lower (87%) with marked variation. In particular, intra-assay precision analysis showed a significant decrease in the coefficient of variation for the gC-gD ELISA (∼4%) compared with the gD (∼15%), gC (∼20%) and whole virus (∼40%) formats, indicating the highest reproducibility of the gC-gD ELISA. Taken together, these results demonstrate that the gC-gD ELISA provides a novel and robust tool for high-throughput AnHV-1 serosurveillance, which represents a major breakthrough in terms of economy when compared to traditional whole virus-based ELISAs for large field applications.","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"1-8"},"PeriodicalIF":2.7,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The virulence of two recent Dutch Mycoplasma synoviae field isolates in different broiler types and the association with ascites and hydropericardium as a new finding. 新近分离的两株荷兰滑膜支原体在不同肉鸡中的毒力及其与腹水和心包膜积液的关系的新发现。

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-09-09 DOI: 10.1080/03079457.2026.2705430

Anneke Feberwee, Hanneke Bataille, Remco Dijkman, Manon Holstege, Christiaan Ter Veen, Jeanine Wiegel, Naomi De Bruijn

{"title":"The virulence of two recent Dutch Mycoplasma synoviae field isolates in different broiler types and the association with ascites and hydropericardium as a new finding.","authors":"Anneke Feberwee, Hanneke Bataille, Remco Dijkman, Manon Holstege, Christiaan Ter Veen, Jeanine Wiegel, Naomi De Bruijn","doi":"10.1080/03079457.2026.2705430","DOIUrl":"10.1080/03079457.2026.2705430","url":null,"abstract":"In the present study, the virulence of two Mycoplasma synoviae (MS) field isolates was evaluated in specific pathogen-free (SPF) and modern-day commercial broilers. Both MS isolates belonged to Multilocus Sequence Typing (MLST) clonal complex (STCplx) 56, the most prevalent STCplx isolated from the respiratory tract of Dutch commercial poultry in the last two decades. Additionally, in this study, a well-defined histologic air sac lesion (ASL) score was combined with an in situ hybridization (ISH) test to further study the cellular reaction in the air sacs and tracheal tissue. Birds were challenged intratracheally with MS at 5 days of age. The synergistic effect of a mild infectious bronchitis virus (IBV) H120 vaccine given 5 days prior to MS challenge was also evaluated in the commercial broilers. The clinical impact of the MS strains was comparable to earlier studies with older isolates, as were the synergistic effects of IBV. The present study demonstrated a difference in effect on growth, macroscopic air sac lesions (mASL) and histologic (h)ASL between the two broiler types. A relevant new finding with economic impact for the poultry industry was that ascites was observed related to both isolates; however, the mechanisms underlying MS-associated ascites require further investigation. The hASL, combined with the MS ISH assay, revealed differences in cellular reaction in the air sacs compared to the trachea, explaining the lower MS detection rate in the air sacs in the chronic phase of MS infection. This also emphasizes the risk of misdiagnosing MS-related airsacculitis in broilers.","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"1-16"},"PeriodicalIF":2.7,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a rapid detection method for Tembusu virus based on MIRA-PfAgo. 基于MIRA-PfAgo的坦布苏病毒快速检测方法的建立。

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-08-27 DOI: 10.1080/03079457.2026.2701311

Bei Wang, Liwei Liu, Xiaoqing Wang, Kai Yang, Daojun Chen, Dongdong Yin

{"title":"Establishment of a rapid detection method for Tembusu virus based on MIRA-PfAgo.","authors":"Bei Wang, Liwei Liu, Xiaoqing Wang, Kai Yang, Daojun Chen, Dongdong Yin","doi":"10.1080/03079457.2026.2701311","DOIUrl":"10.1080/03079457.2026.2701311","url":null,"abstract":"In this study, a novel nucleic acid detection method based on multienzyme isothermal rapid amplification (MIRA) combined with the Pyrococcus furiosus Argonaute (PfAgo) cleavage system (MIRA-PfAgo) was developed for the detection of Tembusu virus (TMUV), an important pathogen threatening the waterfowl industry. The method targets the highly conserved NS5 gene of TMUV. Target nucleic acids are rapidly enriched by MIRA under isothermal conditions at 39°C, followed by PfAgo-mediated cleavage guided by sequence-specific guide DNA (gDNA), enabling visual detection through fluorescence signal output. Systematic optimization identified 0.5 μM gDNA, 1 μM PfAgo, and 5 mM Mn²⁺ as the optimal reaction conditions. The assay exhibited excellent specificity for TMUV, with no cross-reactivity observed with common waterfowl pathogens, including H9 subtype avian influenza virus (AIV-H9), duck enteritis virus (DEV), goose parvovirus (GPV), Muscovy duck reovirus (MDRV), Newcastle disease virus (NDV), and duck circovirus (DuCV). The sensitivity was 10⁰ copies/μl. In the analysis of 21 clinical samples, the results of the MIRA-PfAgo assay were fully consistent with those obtained by RT-PCR. This method does not require complex thermal cycling equipment and combines high sensitivity, strong specificity, and operational simplicity, providing a reliable and practical technical tool for rapid on-site monitoring of TMUV.RESEARCH HIGHLIGHTSThis is the first study to utilize the MIRA-PfAgo detection assay for the detection of TMUV.The results are observable by the unaided eye.The developed assay potentially offers an alternative means for detection of TMUV, requiring minimal equipment.","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"1-9"},"PeriodicalIF":2.7,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148403216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a reassortant infectious bursal disease virus (genotype A3B1) in the United Kingdom: pathogenicity and immune complex vaccine efficacy. 在英国鉴定一种重组传染性法氏囊病病毒（基因型A3B1）：致病性和免疫复合物疫苗效力。

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-08-03 DOI: 10.1080/03079457.2026.2701308

Gregori Bech, Elena Marzo, Christian Blake-Dyke, Marta Busquet, Michelle Woodward, Marta Alech, Marina Solé, Alba Sánchez, Eduard Sebastià

{"title":"Identification of a reassortant infectious bursal disease virus (genotype A3B1) in the United Kingdom: pathogenicity and immune complex vaccine efficacy.","authors":"Gregori Bech, Elena Marzo, Christian Blake-Dyke, Marta Busquet, Michelle Woodward, Marta Alech, Marina Solé, Alba Sánchez, Eduard Sebastià","doi":"10.1080/03079457.2026.2701308","DOIUrl":"10.1080/03079457.2026.2701308","url":null,"abstract":"The immunosuppressive effects of the infectious bursal disease virus (IBDV), together with its ongoing genome evolution, still represent a major challenge for poultry farmers. This study characterized a novel reassortant IBDV strain, named UK2020, which was isolated in broiler farms from the United Kingdom in 2020 and has now spread across Europe. The phylogenetic analysis revealed that UK2020 belongs to the genotype A3B1, with segment A derived from a very virulent strain (vvIBDV) and segment B from a classical attenuated virus. The pathogenicity studies conducted in both specific-pathogen-free and commercial broiler chickens, with and without maternally-derived antibodies (MDAs), demonstrated that UK2020 caused subclinical infections. These infections resulted in splenomegaly, moderate inflammation of the bursa with histopathological changes, such as lymphoid depletion, inflammatory cell infiltration and plical oedema, and a significant humoral response, despite the absence of clinical signs. The efficacy of a commercial IBD immune complex (Icx) vaccine was also evaluated. Vaccinated birds showed significantly higher antibody titres and reduced lymphoid organ damage compared to mock-vaccinated controls. These results highlight the need for continuous field surveillance to monitor the circulation and impact of reassortant IBDV strains under the current control programmes. The complete sequencing of segments A and B is essential for accurate detection and classification of such strains. The use of Icx vaccines may contribute to mitigating bursal and splenic lesions associated with A3B1 infections like UK2020, especially in flocks with variable MDA levels.","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"1-13"},"PeriodicalIF":2.7,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148663481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early challenge with LPAI-H9N2 followed by vNDV-VII.1.1 in vaccinated and unvaccinated broilers: a comprehensive study. 在接种疫苗和未接种疫苗的肉鸡中，早期用LPAI-H9N2攻毒，随后用vNDV-VII.1.1攻毒：一项综合研究

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-08-01 Epub Date: 2026-06-24 DOI: 10.1080/03079457.2026.2674853

Ahmed Elbestawy, Eman Khalifa, Adel H Saad, Ahmed R Elnagar, Hany F Ellakany, Mervat Abdel-Latif, Ahmed E Noreldin, Amr Geneedy, Ahmed Gado

引用次数: 0

Genetic and vaccination studies reveal feather viral load as a potential biomarker for individual and flock-level protection against Marek's disease in chicken. 遗传和疫苗研究表明，羽毛病毒载量是鸡个体和群体预防马立克病的潜在生物标志物。

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-08-01 Epub Date: 2026-06-22 DOI: 10.1080/03079457.2026.2661686

Hector Marina, Richard I Bailey, Jody Mays, Marie Ithurbide, Margo Chase-Topping, Hans Cheng, John R Dunn, Andrea Doeschl-Wilson

引用次数: 0

Editorial introduction. 编辑介绍。

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-08-01 Epub Date: 2026-07-13 DOI: 10.1080/03079457.2026.2677382

Magdy F Elkady, Ahmed R Elbestawy, Kareem Eid

引用次数: 0

Outbreak dynamics of high pathogenicity avian influenza virus H5N1, clade 2.3.4.4b euBB, in black-headed gulls and common terns in Germany in 2023. 2023年德国黑头鸥和普通候鸟中高致病性禽流感病毒H5N1，分支2.3.4.4b euBB的爆发动态

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-08-01 Epub Date: 2026-06-08 DOI: 10.1080/03079457.2026.2659834

Ulrich Knief, Ann Kathrin Ahrens, Valerie Allendorf, Carla J Behringer, Justine Bertram, Sandra Bouwhuis, Wolfgang Fiedler, Anja Globig, Anne Günther, Christof Herrmann, Sascha Knauf, Dominik Marchowski, Simon Piro, Anne Pohlmann, Robert E Rollins, Christoph Staubach, Timm Harder

引用次数: 0

Antibiotic resistance and first comprehensive genomic characteristics of Salmonella Gallinarum isolated from poultry in Morocco. 摩洛哥家禽鸡源沙门氏菌的抗生素耐药性和首次综合基因组特征。

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-08-01 Epub Date: 2025-10-31 DOI: 10.1080/03079457.2025.2562221

Mouad Farhat, Slimane Khayi, Mohamed Mouahid, Najia Ameur, Hosny El-Adawy, Siham Fellahi

引用次数: 0

First identification of concurrent infections with novel emerging duck astrovirus and duck hepatitis A virus type 3 (DHAV-3) in ducklings in Egypt using metagenomic next-generation sequencing (m-NGS). 新出现的鸭星状病毒和鸭甲型肝炎病毒3型（DHAV-3）在埃及雏鸭中的并发感染首次使用宏基因组新一代测序（m-NGS）鉴定。

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-08-01 Epub Date: 2025-11-13 DOI: 10.1080/03079457.2025.2581279

Fouad S El-Mayet, Ibrahim Moharam, Ehab M El-Nahas, Ayman S El-Habbaa, Fares Z Najar, Erin Stayton, Eman M S El Nagar, Medhat Radi, Norhan W Khalil, Mohamed A Abaza

引用次数: 0