Biochemical medicine最新文献_第4页

Acidic metabolites of phenylalanine in plasma of phenylketonurics 苯丙酮尿症患者血浆中苯丙氨酸的酸性代谢物

Biochemical medicine Pub Date : 1985-10-01 DOI: 10.1016/0006-2944(85)90112-7

Mendel Tuchman, Robert O. Fisch, Margaret L. Ramnaraine, William Krivit

引用次数: 14

Inhibition of d-glucose uptake by isatin in rat intestine: Effect of harmaline and various sulfhydryl reagents 茴香素对大鼠肠道d-葡萄糖摄取的抑制作用:甘草碱和各种巯基试剂的作用

Biochemical medicine Pub Date : 1985-10-01 DOI: 10.1016/0006-2944(85)90113-9

J.P. Nagpal , R.K. Wali , R. Singh , S. Farooqui , S. Majumdar , A. Mahmood

引用次数: 2

Targeting of synthetically glycosylated human placental glucocerebrosidase 靶向合成糖基化人胎盘糖脑苷酶

Biochemical medicine Pub Date : 1985-10-01 DOI: 10.1016/0006-2944(85)90117-6

Gary J. Murray , Thomas W. Doebber , T.Y. Shen , M.S. Wu , M.M. Ponpipom , R.L. Bugianesi , Roscoe O. Brady , John A. Barranger

引用次数: 15

Separation of biological variant insulin molecules from different species by reversed-phase high-performance liquid chromatography (HPLC) 反相高效液相色谱法分离不同物种生物变异胰岛素分子

Biochemical medicine Pub Date : 1985-10-01 DOI: 10.1016/0006-2944(85)90116-4

D. Kalant, J.C. Crawhall, B.I. Posner

引用次数: 7

Low content of hepatic reduced glutathione in patients with Wilson's disease 肝豆状核变性患者肝还原性谷胱甘肽含量低

Biochemical medicine Pub Date : 1985-08-01 DOI: 10.1016/0006-2944(85)90067-5

K.H. Summer , J. Eisenburg

引用次数: 44

Aromatic l-amino acid decarboxylase activities in human lung tissues: Comparison between normal lung and lung carcinomas 人肺组织芳香族l-氨基酸脱羧酶活性:正常肺与肺癌的比较

Biochemical medicine Pub Date : 1985-08-01 DOI: 10.1016/0006-2944(85)90061-4

Toshiharu Nagatsu , Hiroshi Ichinose , Kohichi Kojima , Toru Kameya , Junji Shimase , Tetsuro Kodama , Yukio Shimosato

引用次数: 31

The insulin-mimetic action of Mn2+: Involvement of cyclic nucleotides and insulin in the regulation of hepatic hexokinase and glucokinase Mn2+的胰岛素模拟作用:环核苷酸和胰岛素参与肝脏己糖激酶和葡萄糖激酶的调节

Biochemical medicine Pub Date : 1985-08-01 DOI: 10.1016/0006-2944(85)90064-X

Samararatne Subasinghe , A.Leslie Greenbaum , Patricia McLean

{"title":"The insulin-mimetic action of Mn2+: Involvement of cyclic nucleotides and insulin in the regulation of hepatic hexokinase and glucokinase","authors":"Samararatne Subasinghe , A.Leslie Greenbaum , Patricia McLean","doi":"10.1016/0006-2944(85)90064-X","DOIUrl":"10.1016/0006-2944(85)90064-X","url":null,"abstract":"<div><p>Manganese causes a significant rise in hepatic glucokinase and hexokinase in 16-day-old suckling rats, and has an insulinomimetic effect in producing a precocious emergence of glucokinase (EC 2.7.1.2) and a rise in the low <em>K</em><sub><em>m</em></sub>, kexokinases (EC 2.7.1.1) activities. These enzyme changes occur within 6 hr of manganese administration and there are accompanying increases in plasma insulin and hepatic cyclic GMP. That the effect of manganese is at a site other than, or in addition to, insulin secretion is suggested by the significant increases in glucokinase and hexokinase in 16-day-old streptozotocin-diabetic rats; in this group there is also an increase in hepatic cGMP similar in time scale to that of the normal-manganese-treated group. The effects of manganese and insulin were not additive. It is proposed that one site of action of manganese may be at the level of cyclic GMP systems. The results are also discussed in relation to the known action of manganese at the level of the protein phosphatases.</p></div>","PeriodicalId":8781,"journal":{"name":"Biochemical medicine","volume":"34 1","pages":"Pages 83-92"},"PeriodicalIF":0.0,"publicationDate":"1985-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-2944(85)90064-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14129992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Human spleen dihydroorotate dehydrogenase: Properties and partial purification 人脾二氢酸脱氢酶:性质和部分纯化

Biochemical medicine Pub Date : 1985-08-01 DOI: 10.1016/0006-2944(85)90063-8

Annette M. Gero, William J. O'Sullivan

{"title":"Human spleen dihydroorotate dehydrogenase: Properties and partial purification","authors":"Annette M. Gero, William J. O'Sullivan","doi":"10.1016/0006-2944(85)90063-8","DOIUrl":"10.1016/0006-2944(85)90063-8","url":null,"abstract":"<div><p>Human spleen dihydroorotate dehydrogenase is associated with the mitochondrial membrane and is linked to the respiratory chain via ubiquinone. The enzyme activity was unaffected by pyridine nucleotides. The product of the reaction, orotate, was a potent inhibitor. However, a range of other naturally occurring pyrimidines or purines had no significant effect on the activity. No evidence for the involvement of a complexed metal ion or for an active sulfhydryl group was obtained.</p><p>Purification of the enzyme was achieved by preparation of an acetone powder and extraction with Triton X-100, followed by preparative polyacrylamide gel electrophoresis. Activity was observed by the addition of the artificial electron acceptors, ubiquinone 50 or PMS. Purification resulted in alteration of the pH optimum and of other kinetic characteristics. Two molecular-weight species, of molecular weight 88,000 and 98,000, were consistently observed.</p><p>The properties of the human spleen enzyme were similar in principle to those for the rat liver enzyme. Differences in the mode of linkage to the respiratory chain for the mitochondrially bound enzyme, and in the characteristics of the purified enzyme, were observed.</p></div>","PeriodicalId":8781,"journal":{"name":"Biochemical medicine","volume":"34 1","pages":"Pages 70-82"},"PeriodicalIF":0.0,"publicationDate":"1985-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-2944(85)90063-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14129991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

The effect of medroxyprogesterone acetate on the hepatic drug-metabolizing enzymes in normal and protein-deficient female rats 醋酸甲孕酮对正常和蛋白缺乏雌性大鼠肝脏药物代谢酶的影响

Biochemical medicine Pub Date : 1985-08-01 DOI: 10.1016/0006-2944(85)90056-0

J.P. Nagpal , K.L. Khanduja , R.R. Sharma , S. Majumdar , R. Singh , M.P. Gupta , S.C. Dogra

引用次数: 4

Human spleen dihydroorotate dehydrogenase: A study of inhibition of the enzyme 人脾二氢酸脱氢酶:抑制该酶的研究

Biochemical medicine Pub Date : 1985-08-01 DOI: 10.1016/0006-2944(85)90062-6

Annette M. Gero , William J. O'Sullivan , Desmond J. Brown

引用次数: 14