Biochemical medicine最新文献_第3页

Biochemical effects of mild iron deficiency and cold acclimatization on rat skeletal muscle 轻度缺铁和冷适应对大鼠骨骼肌的生化影响

Biochemical medicine Pub Date : 1985-12-01 DOI: 10.1016/0006-2944(85)90098-5

T.L. Quisumbing , T.M. Wong , L.S. Jen , T.T. Loh

{"title":"Biochemical effects of mild iron deficiency and cold acclimatization on rat skeletal muscle","authors":"T.L. Quisumbing , T.M. Wong , L.S. Jen , T.T. Loh","doi":"10.1016/0006-2944(85)90098-5","DOIUrl":"10.1016/0006-2944(85)90098-5","url":null,"abstract":"<div>Most of the previous studies on the effects of iron deficiency on skeletal muscle respiratory capacity and work performance have been investigated in severe or moderate iron-deficiency anemia. We report here that even in mild iron deficiency where the hemoglobin concentration was 10 g/dl and the iron stores in livers and spleen were not completely depleted, a marked reduction in succinate dehydrogenase was observed in skeletal muscles but not in heart. Similarly, cytochrome oxidase activities were reduced. Although no significant change in glycerophosphate dehydrogenase was detected in the iron-deficient rats, exposure to cold in this group greatly reduced this enzyme activity. As cold acclimatization accelerates marrow erythropoiesis (20) which in turn, demands more iron, it seems that in the iron-insufficient state, this iron demand for marrow activity may persist at the expense of the tissue iron pool, resulting in a marked reduction in glycerophosphate dehydrogenase activities. Since succinate dehydrogenase plays a significant role in the impairment of mitochondrial function and early fatigue of iron-deficient muscle (11), the present study shows that even in mild iron deficiency, some loss of muscle functions could result as succinate dehydrogenase activities were greatly reduced.</div>","PeriodicalId":8781,"journal":{"name":"Biochemical medicine","volume":"34 3","pages":"Pages 355-363"},"PeriodicalIF":0.0,"publicationDate":"1985-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-2944(85)90098-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14139878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Analysis of immunoreactive insulins in man in relation to the effects of aging 人体内免疫反应性胰岛素与衰老的关系分析

Biochemical medicine Pub Date : 1985-10-01 DOI: 10.1016/0006-2944(85)90108-5

Keiji Kakita, Sachiko Kakita

引用次数: 2

Oxytocinase-like enzyme in an ovarian dysgerminoma: A placenta-specific protein 卵巢异常生殖细胞瘤中的催产素样酶:一种胎盘特异性蛋白

Biochemical medicine Pub Date : 1985-10-01 DOI: 10.1016/0006-2944(85)90111-5

H. Sakura , H. Kobayashi , S. Tsuruta , S. Mizutani

引用次数: 2

Tracer priming in human protein turnover studies with [15N] glycine [15N]甘氨酸在人类蛋白质周转中的示踪启动研究

Biochemical medicine Pub Date : 1985-10-01 DOI: 10.1016/0006-2944(85)90114-0

Malayappa Jeevanandam, Murray F. Brennan, Glenn D. Horowitz, David Rose, Mardiros H. Mihranian, John Daly, Stephen F. Lowry

{"title":"Tracer priming in human protein turnover studies with [15N] glycine","authors":"Malayappa Jeevanandam, Murray F. Brennan, Glenn D. Horowitz, David Rose, Mardiros H. Mihranian, John Daly, Stephen F. Lowry","doi":"10.1016/0006-2944(85)90114-0","DOIUrl":"10.1016/0006-2944(85)90114-0","url":null,"abstract":"<div>Sixty-three studies in healthy normal volunteers (n = 29), malnourished cancer (n = 8) or non-cancer patients (n = 9), and postoperative radical cystectomy patients (n = 17) were conducted to evaluate the primed constant infusion labeling technique for the estimation of whole-body protein turnover under a variety of dietary conditions. [15N]Glycine was used as the tracer with a prime to infusion ratio of 1300 to 3300 min and a continuous-infusion rate of 0.11 to 0.33 μg 15N · kg−1 · min−1 for 24 to 36 hr. The isotopic steady-state enrichment was reached in all subjects both in urinary urea and ammonia between 10 and 26 hr (mean 18 ± 2). During protein calorie fasting the attainment of isotopic steady state is much quicker (10 to 18 hr) with a primed constant infusion than with a constant infusion alone (≈38 hr). A <math><mtext>P</mtext><mtext>I</mtext></math> ratio greater or less than 1800 (min) usually resulted in a delay of plateau attainment without affecting the protein turnover values. Reliable estimates of protein kinetics in humans can be made in clinical conditions with a 26-hr infusion of glycine at the rate of 0.28 μg 15N · kg−1 · min−1 with a <math><mtext>P</mtext><mtext>I</mtext></math> ratio of 1800 min, collecting six urine samples every 2 hr from 16 hr and analyzing for both urinary urea and ammonia enrichments.</div>","PeriodicalId":8781,"journal":{"name":"Biochemical medicine","volume":"34 2","pages":"Pages 214-225"},"PeriodicalIF":0.0,"publicationDate":"1985-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-2944(85)90114-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15195957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

Bilirubin increases mitochondrial inner membrane conductance 胆红素增加线粒体内膜电导

Biochemical medicine Pub Date : 1985-10-01 DOI: 10.1016/0006-2944(85)90115-2

David A. Stumpf , Luis A. Eguren , Janice K. Parks

引用次数: 9

Protection by plasma proteins against condensed tannin-mediated platelet activation 血浆蛋白对浓缩单宁介导的血小板活化的保护作用

Biochemical medicine Pub Date : 1985-10-01 DOI: 10.1016/0006-2944(85)90110-3

Michael S. Rohrbach , Rebecca A. Rolstad , James A. Russell

引用次数: 3

14C-labeled substrate catabolism by human diploid fibroblasts derived from infants and adults 来自婴儿和成人的人二倍体成纤维细胞的14c标记底物分解代谢

Biochemical medicine Pub Date : 1985-10-01 DOI: 10.1016/0006-2944(85)90109-7

William J. Rhead, Anne Moon, Vickie Roettger, Kimberly Henkle

{"title":"14C-labeled substrate catabolism by human diploid fibroblasts derived from infants and adults","authors":"William J. Rhead, Anne Moon, Vickie Roettger, Kimberly Henkle","doi":"10.1016/0006-2944(85)90109-7","DOIUrl":"10.1016/0006-2944(85)90109-7","url":null,"abstract":"<div>Untransformed diploid skin fibroblasts from eight normal adults, aged 24 to 74 years, catabolized several 14C-labeled substrates less effectively than cells from ten normal male infants. 14C-labeled substrate metabolism was quantitated either by measuring the evolution of 14CO2 from the 14C-labeled compounds or the incorporation of 14C into cellular protein via transamination of tricarboxylic acid cycle intermediates derived from the 14C-labeled substrates. With these methods, adult cells catabolized [1-14C]butyrate, [1-14C]octanoate, and 1-[2-14C]leucine at rates 44 to 64% of those found in infant cells. The oxidation of [1,4-14C]succinate and [U-14C]malate was identical in both infant and adult cells, while [2,3-14C]succinate catabolism was mildly decreased in adult cells (65–80% of control). These observations parallel those made in rat tissues and confirm that the same phenomenon occurs in cultured human fibroblasts.</div>","PeriodicalId":8781,"journal":{"name":"Biochemical medicine","volume":"34 2","pages":"Pages 182-188"},"PeriodicalIF":0.0,"publicationDate":"1985-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-2944(85)90109-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15050253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Acylphosphatase from human skeletal muscle: Purification, some properties and levels in normal and myopathic muscles 人骨骼肌酰基磷酸酶:纯化、正常和肌病肌肉的一些特性和水平

Biochemical medicine Pub Date : 1985-10-01 DOI: 10.1016/0006-2944(85)90107-3

P. Nassi , G. Liguri , N. Landi , A. Berti , M. Stefani , B. Pavolini , G. Ramponi

引用次数: 9

Variations in riboflavin binding by human plasma: Identification of immunoglobulins as the major proteins responsible 人血浆核黄素结合的变化:免疫球蛋白作为主要蛋白质的鉴定

Biochemical medicine Pub Date : 1985-10-01 DOI: 10.1016/0006-2944(85)90106-1

Wendy S.A. Innis, Donald B. McCormick, Alfred H. Merrill Jr.

{"title":"Variations in riboflavin binding by human plasma: Identification of immunoglobulins as the major proteins responsible","authors":"Wendy S.A. Innis, Donald B. McCormick, Alfred H. Merrill Jr.","doi":"10.1016/0006-2944(85)90106-1","DOIUrl":"10.1016/0006-2944(85)90106-1","url":null,"abstract":"<div>Riboflavin binding by plasma proteins from healthy human subjects was examined by equilibrium dialysis using a physiological concentration of [2-14C]riboflavin (0.04 μm). Binding ranged from 0.080 to 0.917 pmole of riboflavin/mg of protein (with a mean ± SD of 0.274 ± 0.206), which corresponded to 4.14 to 49.4 pmole/ml of plasma (15.5 ± 11.0) (N = 34). Males and females yielded similar results. Upon fractionation of plasma by gel filtration, the major riboflavin-binding components eluted with albumin and γ-globulins. Albumin was purified and found to bind riboflavin only very weakly (Kd = 3.8 to 10.4 mm), although FMN and photochemical degradation products (e.g., lumiflavine and lumichrome) were more tightly bound. Binding in the γ-globulin fraction was attributed to IgG and IgA because the binding protein(s) and immunoglobulins copurified using various methods were removed by treatment of plasma with protein A-agarose, and were coincident upon immunoelectrophoresis followed by autoradiography to detect [2-14C]riboflavin. Differences among the plasma samples correlated with the binding recovered with the immunoglobulins. Binding was not directly related to the total IgG or IgA levels of subjects. Hence, it appears that the binding is due to a subfraction of these proteins. These findings suggest that riboflavin-binding immunoglobulins are a major cause of variations in riboflavin binding in human circulation, and may therefore affect the utilization of this micronutrient.</div>","PeriodicalId":8781,"journal":{"name":"Biochemical medicine","volume":"34 2","pages":"Pages 151-165"},"PeriodicalIF":0.0,"publicationDate":"1985-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-2944(85)90106-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15195445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 60

Amino acid content of L5178Y and L5178YL-ASE cells after l-asparaginase treatment l-天冬酰胺酶处理后L5178Y和L5178YL-ASE细胞的氨基酸含量

Biochemical medicine Pub Date : 1985-10-01 DOI: 10.1016/0006-2944(85)90105-X

James F. Keefer , David A. Moraga , Sheldon M. Schuster

{"title":"Amino acid content of L5178Y and L5178YL-ASE cells after l-asparaginase treatment","authors":"James F. Keefer , David A. Moraga , Sheldon M. Schuster","doi":"10.1016/0006-2944(85)90105-X","DOIUrl":"10.1016/0006-2944(85)90105-X","url":null,"abstract":"<div>The amino acid contents of tumor cells that are either sensitive or resistant to treatment with l-asparaginase were measured. These amino acid concentrations were measured as a function of incubation time with l-asparaginase or as a function of the l-asparaginase dose. The cell types compared were the mouse leukemia lines L5178Y (sensitive to l-asparaginase treatment) and L5178Y/l-ASE (resistant to l-asparaginase treatment). Upon l-asparaginase treatment both cell lines lost most of their cellular asparagine but, whereas the resistant cells exhibited the ability to rebound to about 50% of initial values, the sensitive cells did not. While previous work had suggested that asparagine-dependent glycine synthesis was essential for sensitive cells (but not in resistant cells), we found no difference in the glycine content of either of the two cell lines as a function of either time or dose that would support this hypothesis. Major differences between the two cell lines were seen in the content of the essential amino acids before treatment with l-asparaginase. After incubation without l-asparaginase the contents of the two cell lines became similar. These results are discussed in terms of possible mechanisms of l-asparaginase sensitivity and resistance.</div>","PeriodicalId":8781,"journal":{"name":"Biochemical medicine","volume":"34 2","pages":"Pages 135-150"},"PeriodicalIF":0.0,"publicationDate":"1985-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-2944(85)90105-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15195444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5