Biochemical medicine最新文献_第5页

Nature of nonenzymatically bound hexose in hemoglobin, albumin, and crystallin 血红蛋白、白蛋白和结晶蛋白中非酶结合己糖的性质

Biochemical medicine Pub Date : 1985-08-01 DOI: 10.1016/0006-2944(85)90068-7

Anuradha Kumar, K.Krishna Sharma, T.N. Pattabiraman

引用次数: 4

Urinary excretion of modified purines and nucleosides in immunodeficient children 免疫缺陷儿童尿中修饰嘌呤和核苷的排泄

Biochemical medicine Pub Date : 1985-08-01 DOI: 10.1016/0006-2944(85)90060-2

Gordon C. Mills , Frank C. Schmalstieg , Randall M. Goldblum

引用次数: 17

Substrate-induced spectral changes in human normal and chronic myeloid leukemic granulocytes 底物诱导的人正常和慢性髓性白血病粒细胞的光谱变化

Biochemical medicine Pub Date : 1985-08-01 DOI: 10.1016/0006-2944(85)90058-4

Avinash M. Mungikar, Balwant P. Gothoskar

{"title":"Substrate-induced spectral changes in human normal and chronic myeloid leukemic granulocytes","authors":"Avinash M. Mungikar, Balwant P. Gothoskar","doi":"10.1016/0006-2944(85)90058-4","DOIUrl":"10.1016/0006-2944(85)90058-4","url":null,"abstract":"<div><p>Several drugs/chemicals were allowed to interact with the cytochrome <em>P</em>-450 dependent mixed function oxidase system in the postmitochrondrial supernatant fractions of Ficoll-Hypaque-separated granulocytes from human normal subjects and patients with chronic myeloid leukemia. The substrate-induced spectral changes were followed by recording the difference spectra. Compounds conventionally classified as type I and type II substrates, on addition to S<sub>1</sub> fractions of both normal and leukemic granulocytes, caused spectral changes that were <em>reverse</em> to those reported for the rat liver microsomes. Aminopyrine, phenobarbital, and Tween 80 evoked a reverse type I spectral change with a peak at 420–430 nm and a trough at 380–400 nm, whereas aniline and pyridine induced a modified type I (a reverse type II) spectral change characterized by a peak at 408 nm and a trough at 421 nm. These changes were found to be quantitatively proportional to the amounts of substrate added. However, the magnitude of the peaks and troughs was considerably less in the S<sub>1</sub> fraction of the leukemic granulocytes. Correspondingly, total heme content was significantly decreased in S<sub>1</sub> fractions of CML granulocytes as compared to similar fractions of normal granulocytes.</p></div>","PeriodicalId":8781,"journal":{"name":"Biochemical medicine","volume":"34 1","pages":"Pages 22-28"},"PeriodicalIF":0.0,"publicationDate":"1985-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-2944(85)90058-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14130079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of iron deficiency on energy conservation in rat liver and skeletal muscle submitochondrial particles 缺铁对大鼠肝脏和骨骼肌线粒体亚颗粒能量保存的影响

Biochemical medicine Pub Date : 1985-08-01 DOI: 10.1016/0006-2944(85)90065-1

Timothy C. Evans , Bruce Mackler

{"title":"Effect of iron deficiency on energy conservation in rat liver and skeletal muscle submitochondrial particles","authors":"Timothy C. Evans , Bruce Mackler","doi":"10.1016/0006-2944(85)90065-1","DOIUrl":"10.1016/0006-2944(85)90065-1","url":null,"abstract":"<div><p>Submitochondrial particles prepared from liver and skeletal muscle of control and iron-deficient rats were examined for cytochrome content and for both energy-independent and energy-conserving functions. Liver submitochondrial particles appear quite resistant to iron deficiency with cytochrome content and electron-transferring or energy-conserving functions maintained at a level of 85% or better of normal. Iron-deficient skeletal muscle submitochondrial particles, in contrast, have decreased cytochrome content and only 15–20% of the normal capacity for oxidation through either complex I (NADH dehydrogenase) or complex II (succinate dehydrogenase). Energy-linked reactions which involve substrate oxidation/reduction (succinate → NAD<sup>+</sup> reversed electron flow and succinate-driven energy-dependent transhydrogenation) are likewise markedly decreased, while ATP-driven energy-dependent transhydrogenation and mitochondrial ATPase are normal. Our data support the concept that iron deficiency leads to decreased electroncarrying capacity of iron-containing mitochondrial enzymes, with skeletal muscle being much more susceptible than liver, but that the mitochondria are otherwise normal with regard to energy conservation.</p></div>","PeriodicalId":8781,"journal":{"name":"Biochemical medicine","volume":"34 1","pages":"Pages 93-99"},"PeriodicalIF":0.0,"publicationDate":"1985-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-2944(85)90065-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15164238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Recycling enzymatic assay of NAD—Interference by an activator in human erythrocytes 激活剂对人红细胞中nada干扰的循环酶测定

Biochemical medicine Pub Date : 1985-08-01 DOI: 10.1016/0006-2944(85)90070-5

K. Narayanareddy, Bhavani Belavady

引用次数: 0

Catecholamine-stimulated lipolysis in adipocytes of spontaneously hypertensive rats 儿茶酚胺刺激自发性高血压大鼠脂肪细胞的脂肪分解

Biochemical medicine Pub Date : 1985-08-01 DOI: 10.1016/0006-2944(85)90066-3

J.A. Spitzer, A.H. Burns, P.J. O'Malley

{"title":"Catecholamine-stimulated lipolysis in adipocytes of spontaneously hypertensive rats","authors":"J.A. Spitzer, A.H. Burns, P.J. O'Malley","doi":"10.1016/0006-2944(85)90066-3","DOIUrl":"10.1016/0006-2944(85)90066-3","url":null,"abstract":"<div><p>We investigated the catecholamine-stimulated lipolytic response of perirenal adipocytes isolated from spontaneously hypertensive (SHR) and normotensive (C) rats of the Sprague-Dawley (SD) and Wistar-Kyoto (WKY) strain. Younger rats (10–17 weeks) were matched with respect to age and body weight. Age-matched SHR rats were smaller than their C counterparts, had equal-size adipocytes, and demonstrated lipolytic responses equal to C cells. Weight-matched SHR rats were older than normotensive controls, had larger adipocytes, and showed depressed norepinephrine (NE)-stimulated responses with a rightward shift in the doseresponse curve. Rates of lipolysis of SHR and C cells were not different in the simultaneous presence of norepinephrine and theophylline. Nine- to ten-month-old rats were of comparable body weight and adipocyte size regardless of blood pressure status; however, SHR cells still showed a significantly blunted response to catecholamine stimulation. We conclude that (1) the NE-stimulated lipolytic response of adipocytes of SHR rats is significantly less than that elicited from C cells; (2) this function difference seems unrelated to a size difference between cells of younger, SHR and C rats, thus implicating the adrenergic system; and (3) whole body growth (as reflected by body weight) and perirenal adipocyte growth do not proceed in parallel in actively growing SHR rats.</p></div>","PeriodicalId":8781,"journal":{"name":"Biochemical medicine","volume":"34 1","pages":"Pages 100-106"},"PeriodicalIF":0.0,"publicationDate":"1985-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-2944(85)90066-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14130078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Inhibition of citrulline synthesis by octanoate and its modulation by adenine nucleotides 辛酸对瓜氨酸合成的抑制及腺嘌呤核苷酸的调节作用

Biochemical medicine Pub Date : 1985-08-01 DOI: 10.1016/0006-2944(85)90055-9

W.H. Lutz , T.P. Geisbuhler , J.D. Pollack , H.J. McClung , A.J. Merola

{"title":"Inhibition of citrulline synthesis by octanoate and its modulation by adenine nucleotides","authors":"W.H. Lutz , T.P. Geisbuhler , J.D. Pollack , H.J. McClung , A.J. Merola","doi":"10.1016/0006-2944(85)90055-9","DOIUrl":"10.1016/0006-2944(85)90055-9","url":null,"abstract":"<div><p>Liver mitochondria from octanoate-treated rabbits showed an impaired ability to synthesize citrulline. Two methods were used to evaluate citrulline synthesis in rat liver mitochondria. Under these conditions octanoate inhibited citrulline synthesis by over 50%. When ATP was included in the assay medium the inhibitory effect of ocatanoate was prevented. In the absence of ATP in the suspending medium, octanoate did not significantly lower total adenine nucleotides in rat liver mitochondria. However, under these conditions octanoate caused a change in the adenine nucleotide profile such that ATP content was decreased and AMP content was increased. When ATP was present in the assay medium, octanoate caused a similar increase in AMP content. However, ATP decreased only slightly. The alterations in mitochondrial adenine nucleotide profile by octanoate and the reversal of the effect by exogenous ATP suggests that octanoate inhibits citrulline synthesis via reduced intramitochondrial ATP levels. The ability of octanoate to lower mitochondrial ATP and elevate mitochondrial AMP may be related to its intramitochondrial activation by the medium chain fatty acid activating enzyme.</p></div>","PeriodicalId":8781,"journal":{"name":"Biochemical medicine","volume":"34 1","pages":"Pages 1-10"},"PeriodicalIF":0.0,"publicationDate":"1985-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-2944(85)90055-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15163683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The antiarrhythmic action of phosphocreatine in acute myocardial ischemia 磷酸肌酸在急性心肌缺血中的抗心律失常作用

Biochemical medicine Pub Date : 1985-08-01 DOI: 10.1016/0006-2944(85)90069-9

L.V. Rosenshtraukh, V.A. Saks, E.P. Anyukhovsky, G.G. Beloshapko, A.V. Yushmanova

引用次数: 9

Plasma lipoprotein levels and in vitro cholesterol synthesis by cells in human blood 血浆脂蛋白水平与人血液细胞体外胆固醇合成

Biochemical medicine Pub Date : 1985-08-01 DOI: 10.1016/0006-2944(85)90057-2

W.H.F. Sutherland, E.R. Nye

引用次数: 1

Plasma lipoproteins affect platelet malondialdehyde and thromboxane B2 production 血浆脂蛋白影响血小板丙二醛和凝血素B2的产生

Biochemical medicine Pub Date : 1985-08-01 DOI: 10.1016/0006-2944(85)90059-6

Michael Aviram , Cesare R. Sirtori , Susanna Colli , Paola Maderna , Gabriele Morazzoni , Elena Tremoli

{"title":"Plasma lipoproteins affect platelet malondialdehyde and thromboxane B2 production","authors":"Michael Aviram , Cesare R. Sirtori , Susanna Colli , Paola Maderna , Gabriele Morazzoni , Elena Tremoli","doi":"10.1016/0006-2944(85)90059-6","DOIUrl":"10.1016/0006-2944(85)90059-6","url":null,"abstract":"<div><p>Platelet interaction with plasma lipoproteins was studied using gel-filtered platelets free of plasma constituents and purified lipoproteins. On incubation of gel-filtered platelets with plasma lipoproteins at 30°C for 30 min, 100 μg of protein/ml of very-low as well as low-density lipoprotein caused 10% increment in platelet aggregation and [<sup>14</sup>C]serotonin release in parallel to elevation of around 15% of malondialdehyde and thromboxane B<sub>2</sub> production. High-density lipoprotein showed the opposite effect and reduced platelet aggregation as well as thromboxane B<sub>2</sub> synthesis by 17 and 32%, respectively. Lipoprotein-deficient plasma enhanced platelet function. Preincubation of the platelet suspension with prostacyclin did not prevent the effect of the lipoproteins on the <em>in vitro</em> platelet response as well as on the platelet prostaglandin pathway. Our results suggest that the formation of thromboxane B<sub>2</sub> and malondialdlehyde is influenced by plasma lipoproteins and that these, in turn, affect platelet aggregation and the release reaction. The possible significance of these results to platelet function in hyperlipidemic patients is discussed.</p></div>","PeriodicalId":8781,"journal":{"name":"Biochemical medicine","volume":"34 1","pages":"Pages 29-36"},"PeriodicalIF":0.0,"publicationDate":"1985-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-2944(85)90059-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15163688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 43