Biochemical medicine最新文献_第6页

Plasma plasmin inhibitors in duchenne-type progressive muscular dystrophy 血浆纤溶酶抑制剂治疗杜氏型进行性肌营养不良

Biochemical medicine Pub Date : 1985-06-01 DOI: 10.1016/0006-2944(85)90010-9

Isao Shomura, Naruhiko Nagao, Yoshio Sawada

{"title":"Plasma plasmin inhibitors in duchenne-type progressive muscular dystrophy","authors":"Isao Shomura, Naruhiko Nagao, Yoshio Sawada","doi":"10.1016/0006-2944(85)90010-9","DOIUrl":"10.1016/0006-2944(85)90010-9","url":null,"abstract":"<div>In our previous research, we found that the level of the plasminogen activity in the plasma from Duchenne-type patients with progressive muscular dystrophy was higher than of the normal boys, though the level of the plasmin inhibitors was lower. Therefore, in the present study, we investigated the differences in the fractions of plasmin inhibitors.The subjects were nine patients (the average age being 17.1 years) who had been diagnosed, by clinical and biochemical tests, as having PMD; serving as controls were normal boys (the average age being 15 years), the patients' mothers, and the mothers of the normal boys.The plasmin inhibitors were separated from plasma using lysine-Sepharose columns according to the method of Urita et al. (6). The determination was performed based on the method of Aoyagi et al. (7) and an immunoreactive assay.The results were as follows: (1) No significant differences were seen between patients with PMD and control subjects with respect to either α1-antichymotrypsin, antithrombin III, and α1-antitrypsin or α2-macroglobulin and inter-α-trypsin inhibitors. These results suggested that the low level of plasmin inhibitors in patients was due to the low activity of the C1 inactivator. (2) The patients with PMD showed lower values than the normal boys in the levels of C1 inactivator in plasma; similarly, the mothers of these patients showed lower values than the normal mothers.</div>","PeriodicalId":8781,"journal":{"name":"Biochemical medicine","volume":"33 3","pages":"Pages 350-361"},"PeriodicalIF":0.0,"publicationDate":"1985-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-2944(85)90010-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14291019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Functional relationship of ammonia with RNA, DNA, and protein in rat muscle 氨与大鼠肌肉中RNA、DNA和蛋白质的功能关系

Biochemical medicine Pub Date : 1985-06-01 DOI: 10.1016/0006-2944(85)90002-X

M.I. Ghori , K. Subramanyam , B. Moorthy , B. Sadasivudu

引用次数: 1

Regulation of NAD- and NADP-linked isocitrate dehydrogenase by thyroxine in the brain and liver of female rats of various ages 甲状腺素对不同年龄雌性大鼠脑和肝脏NAD-和nadp -连接异柠檬酸脱氢酶的调节作用

Biochemical medicine Pub Date : 1985-06-01 DOI: 10.1016/0006-2944(85)90005-5

R.N.Singh Yadav

引用次数: 4

The isolation of glucose tolerance factors from brewer's yeast and their relationship to chromium 啤酒酵母中葡萄糖耐量因子的分离及其与铬的关系

Biochemical medicine Pub Date : 1985-06-01 DOI: 10.1016/0006-2944(85)90004-3

D.M. Davies, E.S. Holdsworth, J.L. Sherriff

引用次数: 40

Amino acid depletion in the blood and brain tissue of hyperphenylalaninemic rats is abolished by the administration of additional lysine: A contribution to the understanding of the metabolic defects in phenylketonuria 高苯丙氨酸血症大鼠血液和脑组织中的氨基酸消耗被额外赖氨酸的管理所消除:有助于理解苯丙酮尿的代谢缺陷

Biochemical medicine Pub Date : 1985-06-01 DOI: 10.1016/0006-2944(85)90008-0

Gerald Huether, Renate Kaus, Volker Neuhoff

{"title":"Amino acid depletion in the blood and brain tissue of hyperphenylalaninemic rats is abolished by the administration of additional lysine: A contribution to the understanding of the metabolic defects in phenylketonuria","authors":"Gerald Huether, Renate Kaus, Volker Neuhoff","doi":"10.1016/0006-2944(85)90008-0","DOIUrl":"10.1016/0006-2944(85)90008-0","url":null,"abstract":"<div>The elevated phenylalanine concentration in the blood of untreated phenylketonuric children is known to be paralleled by decreased concentrations of other amino acids in the blood and brain tissue. Due to the low availability of other large, neutral amino acids in the brain, protein synthesis in, and the normal development of, the brain are disturbed. A similar effect is observed in suckling rats rendered hyperphenylalaninemic by the daily injection of phenylalanine plus α-methylphenylalanine, an in vivo inhibitor of the phenylalanine-hydroxylating pathway in the liver. In this study, the simultaneous injection of lysine is shown to prevent the depletion of amino acids from the blood and brain tissue, and the retardation of brain growth, in suckling hyperphenylalaninemic rats. It is suggested that both amino acids, phenylalanine and lysine, are important rate-limiting substrates for the rapid protein anabolism of developing tissues. In the presence of an excess of phenylalanine, other amino acids, and in relation to its requirement during the phase of hyperplastic growth in particular lysine, are less available from the circulation and limit phenylalanine-stimulated protein synthesis in developing tissues. The supplementation of lysine to developing hyperphenylalaninemic rats prevents the consequences of this effect, i.e., the depletion of amino acids in the blood, and therefore, in the brain tissue, and the retardation of brain growth.</div>","PeriodicalId":8781,"journal":{"name":"Biochemical medicine","volume":"33 3","pages":"Pages 334-341"},"PeriodicalIF":0.0,"publicationDate":"1985-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-2944(85)90008-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15040441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Quantitation of malondialdehyde (MDA) in plasma, by ion-pairing reverse phase high performance liquid chromatography 离子配对反相高效液相色谱法测定血浆中丙二醛(MDA)含量

Biochemical medicine Pub Date : 1985-06-01 DOI: 10.1016/0006-2944(85)90003-1

C.R. Wade, P.G. Jackson, A.M. Van Rij

引用次数: 53

Studies on the charge isomers of arylsulfatase A 芳基磺化酶A带电荷异构体的研究

Biochemical medicine Pub Date : 1985-06-01 DOI: 10.1016/0006-2944(85)90013-4

Theodore A. Sarafian , Katherine K. Tsay , William E. Jackson , Arvan L. Fluharty , Hayato Kihara

{"title":"Studies on the charge isomers of arylsulfatase A","authors":"Theodore A. Sarafian , Katherine K. Tsay , William E. Jackson , Arvan L. Fluharty , Hayato Kihara","doi":"10.1016/0006-2944(85)90013-4","DOIUrl":"10.1016/0006-2944(85)90013-4","url":null,"abstract":"<div>Human liver arylsulfatase A was resolved into six fractions by narrow pH range preparative isoelectric focusing. Analytical isoelectric focusing revealed that most enzyme fractions were composed of two adjacent charge isomers. Nevertheless, there was considerable enrichment of charge species which allowed a comparative study of selected properties. Except for the most cationic fraction, neuraminidase treatment converted enzyme in all fractions to the three most cationic species. The most electronegative enzyme species had the highest molecular mass being made up of 64-kDa subunits. As electronegativity decreased, there was concomitant decrease in molecular mass and increase in complexity of subunit composition. Two subunits—61 and 55 kDa—prevailed with increasing proportions of the smaller unit with loss of electronegativity. There was also an increasing amount of a 26-kDa fraction which became a substantial component of the most cationic subfraction. Only enzyme in the two fractions containing the largest and most anionic species were taken up by cultured fibroblasts at higher efficiency than unfractionated enzyme. It is suggested that processing or maturation of arylsulfatase A incurs stepwise removal of charge groups and/or peptide segments leading to smaller, less-charged enzyme species.</div>","PeriodicalId":8781,"journal":{"name":"Biochemical medicine","volume":"33 3","pages":"Pages 372-380"},"PeriodicalIF":0.0,"publicationDate":"1985-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-2944(85)90013-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13999591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Collagenase inhibitory activity in human breast milk 人母乳中胶原酶抑制活性

Biochemical medicine Pub Date : 1985-06-01 DOI: 10.1016/0006-2944(85)90001-8

Beverly Waxler , Janice Corey , Frederick H. Wezeman

引用次数: 0

The increase of lipid peroxidation in rat adjuvant arthritis and its inhibition by superoxide dismutase 大鼠佐剂性关节炎脂质过氧化的增加及其超氧化物歧化酶的抑制作用

Biochemical medicine Pub Date : 1985-06-01 DOI: 10.1016/0006-2944(85)90006-7

Toshikazu Yoshikawa, Hironobu Tanaka, Motoharu Kondo

{"title":"The increase of lipid peroxidation in rat adjuvant arthritis and its inhibition by superoxide dismutase","authors":"Toshikazu Yoshikawa, Hironobu Tanaka, Motoharu Kondo","doi":"10.1016/0006-2944(85)90006-7","DOIUrl":"10.1016/0006-2944(85)90006-7","url":null,"abstract":"<div>Adjuvant arthritis was induced in rats by the injection of Mycobacterium tuberculosis, and its severity was scored according to the macroscopic findings of the legs, tail, and ears. The average score so obtained was lower in SOD-injected rats than in the control group. The depression of albumin/globulin ratio was inhibited significantly in rats treated with 10.0 mg/kg of SOD. The levels of acid phosphatase and β-glucuronidase were elevated after the administration of an adjuvant, and these lysosomal enzymes showed a remarkable increase in the control rats, while the elevation was inhibited in rats injected with 10.0 mg/kg of SOD. The levels of TBA-reactive substance in the sera and synovia were elevated at 2 weeks after the injection of adjuvant and decreased thereafter. In rats injected with 5.0 mg/kg or 10.0 mg/kg of SOD, the increase in both serum and synovial levels of TBA reactants was inhibited significantly.These observations suggest that the aggravation of adjuvant arthritis may be associated with lipid peroxidation due to superoxide, and that SOD may be beneficial for the treatment of arthritis.</div>","PeriodicalId":8781,"journal":{"name":"Biochemical medicine","volume":"33 3","pages":"Pages 320-326"},"PeriodicalIF":0.0,"publicationDate":"1985-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-2944(85)90006-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15129660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 66

Heterogeneity of tissue sterols and glycerolipids in Wolman's disease 组织固醇和甘油脂在沃尔曼病中的异质性

Biochemical medicine Pub Date : 1985-06-01 DOI: 10.1016/0006-2944(85)90009-2

Hsiang Ju Lin , Faith C.S. Ho , Edwin C.L. Yu , Siu-Wah Pang

引用次数: 3