Biochemistry and Cell Biology最新文献_第3页

miR-20b-5p inhibits inflammation-induced endoplasmic reticulum stress through EIF2S1 and promotes osteogenic differentiation of bone marrow mesenchymal stem cells. miR-20b-5p通过EIF2S1抑制炎症诱导的内质网应激，促进骨髓间充质干细胞成骨分化。

IF 2.2 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2025-0351

Zhipeng Pan, Qingsen Zhao, Piao Li, Meiwen Ding, Ya Li, Liangmin Gao, Yunxiang Xiong, Kaihua Tang, Chunyan Gong, Liqing Yao

{"title":"miR-20b-5p inhibits inflammation-induced endoplasmic reticulum stress through EIF2S1 and promotes osteogenic differentiation of bone marrow mesenchymal stem cells.","authors":"Zhipeng Pan, Qingsen Zhao, Piao Li, Meiwen Ding, Ya Li, Liangmin Gao, Yunxiang Xiong, Kaihua Tang, Chunyan Gong, Liqing Yao","doi":"10.1139/bcb-2025-0351","DOIUrl":"10.1139/bcb-2025-0351","url":null,"abstract":"Bone marrow mesenchymal stem cells (BMSCs) play crucial roles in bone tissue regeneration and repair due to their self-renewal ability and multidifferentiation potential. Additionally, miRNAs play crucial roles in controlling osteogenic differentiation. The purpose of this study was to investigate the impact of miR-20b-5p on the osteogenic differentiation of BMSCs under inflammatory conditions. Human BMSCs were treated with 1 µg/mL lipopolysaccharide (LPS) for 24 h to establish an in vitro inflammatory model simulating inflammation in bone injury. The levels of miR-20b-5p, proteins, and inflammatory cytokines were detected via RT-qPCR, Western blotting, and ELISA, and osteogenic differentiation of the BMSCs was evaluated via ALP and alizarin red staining. Consistent with previous findings, LPS downregulated osteogenic markers (RUNX2, OCN, OPN) and upregulated inflammatory cytokines (TNF-α, IL-6, and IL-1β) in BMSCs. Furthermore, the levels of miR-20b-5p were decreased, and the levels of endoplasmic reticulum stress-related proteins (p-EIF2S1, ATF4, CHOP, and GRP78) were increased in LPS-induced BMSCs. However, overexpression of miR-20b-5p weakened the effect of LPS and promoted osteogenic differentiation. Mechanistically, miR-20b-5p overexpression alleviated ER stress caused by LPS-induced inflammation by decreasing EIF2S1 levels, thereby promoting the osteogenic differentiation of BMSCs. Our research indicates that increasing miR-20b-5p expression could be an innovative approach to promote osteogenic differentiation of BMSCs.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-11"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147369113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Note of appreciation. 表示感谢。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2025-0402

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Meeting report: 10th Annual Canadian Conference on Epigenetics. 会议报告：第10届加拿大表观遗传学年会。

IF 2.2 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2026-0013

Shaghayegh Nouruzi, Sabrina Grégoire, Mathieu Lupien, Martin Hirst

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Reply to the discussion of "Efficacy of lactoferrin supplementation in pediatric infections: a systematic review and metanalysis". 回复“补充乳铁蛋白对儿童感染的疗效：一项系统回顾和荟萃分析”的讨论。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2026-0032

Victor D Torres Roldan, Valerie S Mayorga, Rafaella Navarro, Meritxell Urtecho, Theresa J Ochoa

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In vitro and in vivo anticancer efficacy of the combination of actinomycin D and resveratrol. 放线菌素D与白藜芦醇联合应用的体内外抗癌效果。

IF 2.2 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2025-0310

Lukmon M Raji, Rejina Afrin, Jihan Ferdaus Amin, Rajan Lamichhane, Krista L Denning, A R M Ruhul Amin

{"title":"In vitro and in vivo anticancer efficacy of the combination of actinomycin D and resveratrol.","authors":"Lukmon M Raji, Rejina Afrin, Jihan Ferdaus Amin, Rajan Lamichhane, Krista L Denning, A R M Ruhul Amin","doi":"10.1139/bcb-2025-0310","DOIUrl":"10.1139/bcb-2025-0310","url":null,"abstract":"Actinomycin D (Act D) was the first FDA-approved anticancer antibiotic. However, its widespread application was hindered by dose-limiting toxicities. In the current study, we investigated the in vitro and in vivo anticancer efficacy of the combination of Act D with resveratrol. We found that the combination of Act D and resveratrol has better in vitro efficacy than any of the single agent. We also explored the underlying mechanism by investigating the activation of the p53 pathway. Consistent with the cell growth inhibition, Act D, resveratrol, and their combination activated p53 and induced the expression of p21, PUMA, and GDF15 mRNA and proteins. The combination had better effects than any of the single agents alone. Ablation of p53 significantly protected cells and confirmed p53-dependent expression of these genes. Finally, we investigated the in vivo efficacy of these agents and their combination using xenograft model. Act D, resveratrol, and their combination significantly inhibited the growth of xenografts. The in vivo efficacy was further supported by Ki-67 expression in tumor tissues. Taken together, our findings demonstrated that although the combination of Act D and resveratrol showed better efficacy in vitro than any of the single agent, the in vivo efficacy was not improved.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-11"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145931942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Acyl-protein thioesterase 1 (LYPLA1) activity promotes the growth of MDA-MB-468 triple-negative breast cancer cells. 酰基蛋白硫酯酶1 （LYPLA1）活性促进MDA-MB-468三阴性乳腺癌细胞的生长。

IF 2.2 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2025-0446

Michael Salsaa, Mahtab Tavasoli, Haggag S Zein, Shubhashree Pani, Rahul S Kathayat, Bryan C Dickinson, Gregory D Fairn

{"title":"Acyl-protein thioesterase 1 (LYPLA1) activity promotes the growth of MDA-MB-468 triple-negative breast cancer cells.","authors":"Michael Salsaa, Mahtab Tavasoli, Haggag S Zein, Shubhashree Pani, Rahul S Kathayat, Bryan C Dickinson, Gregory D Fairn","doi":"10.1139/bcb-2025-0446","DOIUrl":"10.1139/bcb-2025-0446","url":null,"abstract":"Protein S-acylation is a lipid-based, often reversible post-translational modification that can regulate many aspects of protein behavior, including subcellular localization, protein-interactions, and activity. Emerging evidence has identified roles for individual protein acyltransferases encoded by the ZDHHC in cancers, yet the roles of de-S-acylation enzymes are less clear. Recent evidence suggests that acyl-protein thioesterase (APT1)/LYPLA1 can impact epithelial-mesenchymal transition and metastasis. This study integrates patient datasets, CRISPR dependency data, and in vitro assays to find APT1 as a context-dependent vulnerability in triple-negative breast cancer (TNBC). Despite the highest protein abundance in luminal A MCF7 cells, basal-like MDA-MB-468 cells exhibited the most prominent specific APT1 activity, reflecting subtype-specific regulation. Inhibition of APT1 with ML348 increased S-acylation of nuclear and mitochondrial proteins without altering global acylation. Functionally, APT1 inhibition reduced cell proliferation while inducing minimal apoptosis, consistent with cytostatic growth arrest. Cell-cycle analysis revealed G1 accumulation and reduced S/G2 transition, linking proteomic changes to impaired replication. These findings establish APT1 as a regulator of TNBC proliferation through dynamic de-S-acylation of cell-cycle and mitochondrial proteins, highlighting it as a potential therapeutic vulnerability in aggressive breast cancers.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-12"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147697539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ceramide 1-phosphate binding proteins: emerging regulators of cell physiology and disease. 神经酰胺1-磷酸盐结合蛋白：细胞生理和疾病的新兴调节因子。

IF 2.2 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2026-0193

Antonio Gomez-Muñoz, Asier Benito-Vicente, Kepa B Uribe, Asier Larrea-Sebal, Ana Gomez-Larrauri, César Martín

{"title":"Ceramide 1-phosphate binding proteins: emerging regulators of cell physiology and disease.","authors":"Antonio Gomez-Muñoz, Asier Benito-Vicente, Kepa B Uribe, Asier Larrea-Sebal, Ana Gomez-Larrauri, César Martín","doi":"10.1139/bcb-2026-0193","DOIUrl":"10.1139/bcb-2026-0193","url":null,"abstract":"Ceramide-1-phosphate (C1P), once considered a minor metabolic intermediate, is now recognized as a relevant regulator of cell survival, inflammation, migration, oxidative stress, glucose uptake, and adipogenesis. These diverse functions are mediated by direct interactions between C1P and specific protein targets, including enzymes, lipid transfer proteins, and signaling effectors (typically proteins). While C1P was demonstrated to regulate a variety of signaling pathways, including phosphatidylinositol 3-kinase (PI3K)/Akt, mitogen-activated protein kinase kinase (MEK)/extracellularly regulated kinases (ERK) 1-2, or sphingomyelin synthase (SMS)/protein kinase-C-alpha (PKCα), this review namely focuses on the eight major proteins that directly interact with C1P: acidic sphingomyelinase (ASMase), serine palmitoyl transferase (SPT), Ca2+-dependent cytosolic phospholipase A2-alpha (cPLA2α), Gi protein-coupled receptors, C1P transfer protein (CPTP), sphingomyelinase phosphodiesterase like 3b (SMPDL3b), annexin A2-p11 (S100A10), and Kelch-like ECH-associated protein 1 (KEAP1). Understanding C1P-protein interactions provides a conceptual framework for targeting sphingolipid signaling in inflammation, cancer and metabolic diseases. Here, we summarize current evidence for C1P binding or functional modulation of these proteins, and highlight the implications of these interactions in inflammation, oxidative stress control, and disease pathogenesis.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-12"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148917780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond phenylalanine to tyrosine: Mapping the comprehensive phenylalanine hydroxylase (PAH) pathway for systems biology. 超越苯丙氨酸到酪氨酸：绘制系统生物学的综合苯丙氨酸羟化酶（PAH）途径。

IF 2.2 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2026-0136

Sophia M Khan, Madison L Fennell, Elizabeth Kandiotis, Vanessa Bonura, Jaylin McNeil, Melody Nikseresht, Ceara Olsen, Gemma Sauder, Bronwyn Taylor, Clara E Cho, Jennifer M Monk, Justine R Keathley

{"title":"Beyond phenylalanine to tyrosine: Mapping the comprehensive phenylalanine hydroxylase (PAH) pathway for systems biology.","authors":"Sophia M Khan, Madison L Fennell, Elizabeth Kandiotis, Vanessa Bonura, Jaylin McNeil, Melody Nikseresht, Ceara Olsen, Gemma Sauder, Bronwyn Taylor, Clara E Cho, Jennifer M Monk, Justine R Keathley","doi":"10.1139/bcb-2026-0136","DOIUrl":"10.1139/bcb-2026-0136","url":null,"abstract":"The phenylalanine hydroxylase (PAH) pathway is an enzymatic pathway focused primarily on the hydroxylation of the amino acid L-phenylalanine (Phe) to L-tyrosine (Tyr). However, the functioning of this pathway can also have significant impacts on other downstream metabolites. In addition, both common and rare genetic variants have been demonstrated to significantly impair PAH pathway function. However, to our knowledge, the comprehensive PAH pathway including influences on related downstream metabolic pathways has not been mapped in an integrative manner. As such, we conducted a narrative review of the literature to describe the comprehensive PAH pathway, including influences on downstream related metabolic pathways, while mapping a visual depiction of these integrative processes. In addition to the hydroxylation of Phe to Tyr, the comprehensive pathway narrative describes Phe metabolism and transport across the blood brain barrier, including competition with other large neutral amino acids, Tyr catabolism, and others. Discussion of the importance of these metabolic processes in the context of PAH genetic variation are also provided. With an increased focus on conducting health research from a systems biology perspective, this work is critical to improving our understanding of the downstream effects of amino acid metabolism and their possible impacts on health outcomes.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-11"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148683276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The universally conserved NTPase OLA1. 普遍保守的ntpase ola1。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2025-0347

Jessica Semmelrock, Hans-Joachim Wieden

{"title":"The universally conserved NTPase OLA1.","authors":"Jessica Semmelrock, Hans-Joachim Wieden","doi":"10.1139/bcb-2025-0347","DOIUrl":"10.1139/bcb-2025-0347","url":null,"abstract":"The non-canonical translation factor OBG-like ATPase 1 (OLA1) is conserved across all domains of life. OLA1 has been studied for decades by many groups, often under different names such as GBP45, GTBP9, DOC45, PTD004, EngD, YyaF, Ybr025c, and in bacteria, YchF. Studies have correlated OLA1 with iron metabolism, oxidative stress response, mediation of temperature stress, and virulence. OLA1 has gained attention for its implicated role in a wide range of health conditions and cellular processes including cancer, centrosome regulation/cell proliferation, heart disease, persistent pulmonary hypertension of the newborn, mitochondrial function, neuronal differentiation, protein degradation, atherosclerosis, Down's syndrome, as well as ribosome-dependent protein synthesis. On the background of such a wide range of functional implications, insight into the molecular mechanism of the evolutionarily ancient OLA1 will help to explain the breadth of phenotypic traits. Information about the different protein structural domains present in OLA1 and results from OLA1 in vivo and in vitro studies suggest a role in ribosome-dependent translation regulation based on molecular mimicry with the canonical translation factors.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-18"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147697471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ChestCovidNet: an effective deep learning based approach for COVID-19, lung opacity, and pneumonia detection using chest radiograph images. ChestCovidNet：基于 DL 的有效方法，利用胸片图像检测 COVID-19、肺不张和肺炎

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2023-0265

Naeem Ullah, Javed Ali Khan, Sultan Almakdi, Mohammed S Alshehri, Mimonah Al Qathrady, Muhammad Shahid Anwar, Ikram Syed

{"title":"ChestCovidNet: an effective deep learning based approach for COVID-19, lung opacity, and pneumonia detection using chest radiograph images.","authors":"Naeem Ullah, Javed Ali Khan, Sultan Almakdi, Mohammed S Alshehri, Mimonah Al Qathrady, Muhammad Shahid Anwar, Ikram Syed","doi":"10.1139/bcb-2023-0265","DOIUrl":"10.1139/bcb-2023-0265","url":null,"abstract":"Currently used lung disease screening tools are expensive in terms of money and time. Therefore, chest radiograph images (CRIs) are employed for prompt and accurate COVID-19 identification. Recently, many researchers have applied deep learning (DL) based models to detect COVID-19 automatically. However, their model could have been more computationally expensive and less robust, i.e., its performance degrades when evaluated on other datasets. This study proposes a trustworthy, robust, and lightweight network (ChestCovidNet) that can detect COVID-19 by examining various CRI datasets. The ChestCovidNet model has only 11 learned layers, 8 convolutional (Conv) layers, and 3 fully connected (FC) layers. The framework employs both the Conv and group Conv layers, Leaky Relu activation function, shufflenet unit, Conv kernels of 3 × 3 and 1 × 1 to extract features at different scales, and two normalization procedures that are cross-channel normalization and batch normalization, which make it a novel COVID-19 identification framework. We used data augmentation techniques to increase the number of images and validate the generalizability of the suggested model. We used 9013 CRIs for training, whereas 3863 CRIs for testing the proposed ChestCovidNet approach. Furthermore, we compared the classification results of the proposed framework with hybrid methods in which we employed DL frameworks for feature extraction and support vector machines (SVM) for classification. The study's findings demonstrated that the embedded low-power ChestCovidNet model worked well and achieved a classification accuracy of 98.12% and recall, F1-score, and precision of 95.75% for the four class classifications of CRIs (COVID-19, normal, pneumonia, and lung opacity (LO)).","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-16"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139671221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0