Biochemistry and Cell Biology最新文献_第2页

20 years of choices: a fight for increased funding for graduate students. 20 年的选择：为增加研究生经费而奋斗。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-10-01 Epub Date: 2024-08-27 DOI: 10.1139/bcb-2024-0029

Fabian Rohden, Thomas Bailey, Sarah Laframboise

引用次数: 0

Lactoferrin efficacy in treating hyperferritinemia in patients suffering from pathologies unrelated to hereditary hemochromatosis. 乳铁蛋白对治疗与遗传性血色素沉着病无关的高铁蛋白血症患者的疗效。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-10-01 Epub Date: 2024-07-09 DOI: 10.1139/bcb-2024-0061

Giuditta Pollio, Luigi Rosa, Anna Maria Costanzo, Rosalba Paesano, Giovanni Tripepi, Piera Valenti

{"title":"Lactoferrin efficacy in treating hyperferritinemia in patients suffering from pathologies unrelated to hereditary hemochromatosis.","authors":"Giuditta Pollio, Luigi Rosa, Anna Maria Costanzo, Rosalba Paesano, Giovanni Tripepi, Piera Valenti","doi":"10.1139/bcb-2024-0061","DOIUrl":"10.1139/bcb-2024-0061","url":null,"abstract":"Ferritin (Ftn), a globular protein, sequesters 4500 atoms of iron per molecule. Elevated serum Ftn levels (hyperferritinemia) is an indicator of iron homeostasis disorders. We present the results of an observational study involving 17 patients with hyperferritinemia unrelated to hereditary hemochromatosis (HH). All participants received treatment with 200 mg of bovine lactoferrin (bLf) once (n = 14) or twice (n = 3) a day before meals. The patients, treated with 200 mg/day of bLf, exhibited a significant increase in red blood cells (+10%, p < 0.001), hemoglobin (+4%, p < 0.001), and hematocrit (+15%, p = 0.004), accompanied by a significant reduction in serum Ftn levels (-52%, p < 0.001), C-reactive protein (CRP) (-85.0%, p < 0.001), and D-dimers (-19%, p < 0.001). Among the three patients treated with 400 mg/day of bLf, two had effects similar to those of patients bLf-treated with 200 mg/day and one experienced a strong reduction of Ftn, CRP, and erythrocyte sedimentation rate (from -97% to -75%). The decrease in serum Ftn levels due to bLf treatment was largely independent of gender (p = 0.78), age (p = 0.66), baseline symptoms (p = 0.20), and concomitant acute (p = 0.34) and chronic (p = 0.53) infections. Although this observational pilot study yields positive effects in patients with hyperferritinemia unrelated to HH treated with bLf, a larger sample size is needed for conclusive results.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141562566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Canada's contributions to RNA research: past, present, and future perspectives. 加拿大对核糖核酸研究的贡献：过去、现在和未来的展望。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-09-25 DOI: 10.1139/bcb-2024-0176

Eric Lécuyer, Martin Sauvageau, Ute Kothe, Peter J Unrau, Masad J Damha, Jonathan Perreault, Sherif Abou Elela, Mark A Bayfield, Julie M Claycomb, Michelle S Scott

{"title":"Canada's contributions to RNA research: past, present, and future perspectives.","authors":"Eric Lécuyer, Martin Sauvageau, Ute Kothe, Peter J Unrau, Masad J Damha, Jonathan Perreault, Sherif Abou Elela, Mark A Bayfield, Julie M Claycomb, Michelle S Scott","doi":"10.1139/bcb-2024-0176","DOIUrl":"10.1139/bcb-2024-0176","url":null,"abstract":"The field of RNA research has provided profound insights into the basic mechanisms modulating the function and adaption of biological systems. RNA has also been at the center stage in the development of transformative biotechnological and medical applications, perhaps most notably was the advent of mRNA vaccines that were critical in helping humanity through the Covid-19 pandemic. Unbeknownst to many, Canada boasts a diverse community of RNA scientists, spanning multiple disciplines and locations, whose cutting-edge research has established a rich track record of contributions across various aspects of RNA science over many decades. Through this position paper, we seek to highlight key contributions made by Canadian investigators to the RNA field, via both thematic and historical viewpoints. We also discuss initiatives underway to organize and enhance the impact of the Canadian RNA research community, particularly focusing on the creation of the not-for-profit organization RNA Canada ARN. Considering the strategic importance of RNA research in biology and medicine, and its considerable potential to help address major challenges facing humanity, sustained support of this sector will be critical to help Canadian scientists play key roles in the ongoing RNA revolution and the many benefits this could bring about to Canada.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure and Function of Fermentation-Derived Bovine Lactoferrin Produced from Komagataella phaffii. 由 Komagataella phaffii 发酵产生的牛乳铁蛋白的结构和功能。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-09-19 DOI: 10.1139/bcb-2024-0105

Emma Skoog, Vanessa Feher Castagna, Shafraz Omer, Julianna Madigan, Victoria Flagg, Kristen Burrick, Rulan Jiang, Xiaogu Du, Bo Lönnerdal, Aletta Schnitzler

{"title":"Structure and Function of Fermentation-Derived Bovine Lactoferrin Produced from Komagataella phaffii.","authors":"Emma Skoog, Vanessa Feher Castagna, Shafraz Omer, Julianna Madigan, Victoria Flagg, Kristen Burrick, Rulan Jiang, Xiaogu Du, Bo Lönnerdal, Aletta Schnitzler","doi":"10.1139/bcb-2024-0105","DOIUrl":"https://doi.org/10.1139/bcb-2024-0105","url":null,"abstract":"Bovine lactoferrin (bLf) confers significant functional benefits for human health, but low concentrations in milk and high cost of commercial production limit availability and thus product application. Precision fermentation offers a solution to increase availability of biosimilar recombinant bLf (rbLf) thereby opening new opportunities for this high-value ingredient. To comply with regulatory requirements, we aimed to establish that rbLf from K. phaffii is substantially similar to native bLf in structure and key functions. Intact mass analysis showed a molecular weight of 84 kDa for rbLf, comparable to 82-83 kDa of bLf. LC-MS N-linked glycan profiling revealed predominantly high-mannose-based glycans on rbLf, similar to ~50% of bLf glycans. The isoelectric point and core amino acid sequence of rbLf and bLf are identical. rbLf retains the functional ability to bind and release iron, bind to intestinal Lf receptors, increase epithelial cell growth (>120% of control, P < 0.0001), reduce EPEC growth (>50% reduction, P < 0.0001), bind LPS (+4 fold, P < 0.001) and antagonize LPS-induced TLR4 activity (>40% reduction, P < 0.0001). These results demonstrate similarity of rbLf in structure and function to native bLf, supporting the effective application for expanded market opportunities for infant and adult health.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurodevelopmental functions and activities of the KAT3 class of lysine acetyltransferases. KAT3类赖氨酸乙酰转移酶的神经发育功能和活性。

IF 2.9 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-09-18 DOI: 10.1139/bcb-2024-0156

Ashraf K Shahib,Mojgan Rastegar,Andre J van Wijnen,James R Davie

{"title":"Neurodevelopmental functions and activities of the KAT3 class of lysine acetyltransferases.","authors":"Ashraf K Shahib,Mojgan Rastegar,Andre J van Wijnen,James R Davie","doi":"10.1139/bcb-2024-0156","DOIUrl":"https://doi.org/10.1139/bcb-2024-0156","url":null,"abstract":"The human lysine acetyltransferases KAT3A (CREBBP) and KAT3B (EP300) are essential enzymes in gene regulation in the nucleus. Their ubiquitous expression in metazoan cell types controls cell proliferation and differentiation during development. This comprehensive review delves into the biological roles of KAT3A and KAT3B in neurodevelopment, shedding light on how alterations in their regulation or activity can potentially contribute to a spectrum of neurodegenerative diseases (e.g. Huntington's and Alzheimer's). We explore the pathophysiological implications of KAT3 function loss in these disorders, considering their conserved protein domains and biochemical functions in chromatin regulation. The discussion also underscores the crucial role of KAT3 proteins and their substrates in supporting the integration of key cell signaling pathways. Furthermore, the narrative highlights the interdependence of KAT3-mediated lysine acetylation with lysine methylation and arginine methylation. From a cellular perspective, KAT3-dependent signal integration at subnuclear domains is mediated by liquid-liquid phase separation in response to KAT3-mediated lysine acetylation. The disruption of these finely tuned regulatory processes underscores their pathological roles in neurodegeneration. This review also points to the exciting potential for future research in this field, inspiring further investigation and discovery in the area of neurodevelopment and neurodegenerative diseases.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142255908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bovine lactoferrin and chimera lactoferrin prevent and destroy Salmonella Typhimurium biofilms in Caco-2 cells. 牛乳铁蛋白和嵌合体乳铁蛋白可防止和破坏 Caco-2 细胞中的鼠伤寒沙门氏菌生物膜。

IF 2.9 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-09-18 DOI: 10.1139/bcb-2024-0100

Laura E Quintero-Martínez,Adrian Canizalez-Roman,Uriel A Angulo-Zamudio,Hector Flores-Villaseñor,Jorge A Velazquez-Roman,Jan G M Bolscher,Kamran Nazmi,Nidia Leon-Sicairos

{"title":"Bovine lactoferrin and chimera lactoferrin prevent and destroy Salmonella Typhimurium biofilms in Caco-2 cells.","authors":"Laura E Quintero-Martínez,Adrian Canizalez-Roman,Uriel A Angulo-Zamudio,Hector Flores-Villaseñor,Jorge A Velazquez-Roman,Jan G M Bolscher,Kamran Nazmi,Nidia Leon-Sicairos","doi":"10.1139/bcb-2024-0100","DOIUrl":"https://doi.org/10.1139/bcb-2024-0100","url":null,"abstract":"Salmonellosis is a common foodborne disease caused by Salmonella bacteria. The emergence of multidrug-resistant (MDR) Salmonella serotypes, such as Typhimurium, and Salmonella's ability to form biofilms contribute to their resistance and persistence in host and non-host environments. New strategies are needed to treat or prevent Salmonella infections. This work aimed to determine the effect of the bovine lactoferrin (bLF) and lactoferrin chimera (LFchimera) in preventing or disrupting biofilms formed on abiotic surfaces or Caco-2 cells by Salmonella Typhimurium ATCC 14028 or an MDR strain. The inhibitory activity of planktonic bacteria, prevention of biofilm formation, and destruction of biofilms of S. Typhimurium (ATCC 14028 or MDR strain) on the abiotic surface and Caco-2 cells of bLF and LFchimera were quantified by CFU/ml and visualized by microscopy using Giemsa-stained samples. bLF (75-1000µM) and LFchimera (1-20µM) inhibited more than 95% of S. Typhimurium planktonic growth cultures (ATCC 14028 and MDR). In addition, bLF (600, 800, and 1000 µM) and LFchimera (10 and 20µM) prevented more than 98% of S. Typhimurium adherence and biofilm formation on Caco-2 cells. Finally, bLF (600 and 1000 µM) and LFchimera (10 and 20µM) destroyed more than 80% of S. Typhimurium biofilms established on abiotic and Caco-2 cells. In conclusion, bLF and LF chimeras have the potential to inhibit and destroy S. Typhimurium biofilms.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142255907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishing validated RT-qPCR workflow for the analysis of oligodendrocyte gene expression in the developing murine brain. 建立有效的 RT-qPCR 工作流程，用于分析发育中鼠脑中少突胶质细胞基因的表达。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-08-08 DOI: 10.1139/bcb-2024-0088

Samantha Smith, Emma R Swan, Kendra L Furber

{"title":"Establishing validated RT-qPCR workflow for the analysis of oligodendrocyte gene expression in the developing murine brain.","authors":"Samantha Smith, Emma R Swan, Kendra L Furber","doi":"10.1139/bcb-2024-0088","DOIUrl":"10.1139/bcb-2024-0088","url":null,"abstract":"Myelination is essential for the proper conduction of impulses across neuronal networks. Mature, myelinating glia differentiate from progenitor cells through distinct stages that correspond to oligodendrocyte-specific gene expression markers. Reverse transcription quantiatative PCR (RT-qPCR) is a common technique used to quantify gene expression across cell development; however, a lack of standardization and transparency in methodology may lead to irreproducible data. Here, we have designed and validated RT-qPCR assays for oligodendrocyte genes and reference genes in the developing C57BL6/J mouse brain that align with the MIQE guidelines, including quality controls for primer specificity, temperature dependence, and efficiency. A panel of eight commonly used reference genes was ranked using a series of reference gene stability methods that consistently identified Gapdh, Sdha, Hmbs, Hprt1, and Pgk1 as the top candidates for normalization across brain regions. In the cerebrum, myelin genes peaked in expression at postnatal day 21, which corresponds to the peak of developmental myelination. The gene expression patterns from the brain homogenate were in agreement with previously reported RNA-seq and microarray profiles from oligodendrocyte lineage cells. The validated RT-qPCR assays begin to build a framework for future investigation into the molecular mechanisms that regulate myelination in mouse models of brain development, aging, and disease.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141905788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CD38 deficiency prevents diabetic nephropathy by inhibiting lipid accumulation and oxidative stress through activation of the SIRT3 pathway. CD38 缺乏症可通过激活 SIRT3 途径抑制脂质积累和氧化应激，从而预防糖尿病肾病。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-08-08 DOI: 10.1139/bcb-2024-0058

Lingfang Wang, Qian Li, Jia Le Zhao, Ke Wen, Ya-Ting Zhang, Qi-Hang Zhao, Qi Ding, Jia-Hui Li, Xiao-Hui Guan, Yun-Fei Xiao, Ke Yu Deng, Hong-Bo Xin

{"title":"CD38 deficiency prevents diabetic nephropathy by inhibiting lipid accumulation and oxidative stress through activation of the SIRT3 pathway.","authors":"Lingfang Wang, Qian Li, Jia Le Zhao, Ke Wen, Ya-Ting Zhang, Qi-Hang Zhao, Qi Ding, Jia-Hui Li, Xiao-Hui Guan, Yun-Fei Xiao, Ke Yu Deng, Hong-Bo Xin","doi":"10.1139/bcb-2024-0058","DOIUrl":"https://doi.org/10.1139/bcb-2024-0058","url":null,"abstract":"Diabetic nephropathy (DN) is one of the most common complications of diabetes. Our previous study showed that CD38 knockout (CD38KO) mice had protective effects on many diseases. However, the roles and mechanisms of CD38 in DN remain unknown. Here, DN mice were generated by HFD feeding plus streptozotocin (STZ) injection in male CD38KO and CD38flox mice. Mesangial cells (SV40 MES 13 cells) were used to mimic the injury of DN with palmitic acid (PA) treatment in vitro. Our results showed that CD38 expression was significantly increased in kidney of diabetic CD38flox mice and SV40 MES 13 cells treated with PA. CD38KO mice were significantly resistant to diabetes-induced renal injury. Moreover, CD38 deficiency markedly decreased HFD/STZ-induced lipid accumulation, fibrosis and oxidative stress in kidney tissue. In contrast, overexpression of CD38 aggravated PA-induced lipid accumulation and oxidative stress. CD38 deficiency increased expression of SIRT3, while overexpression of CD38 decreased its expression. More importantly, 3-TYP, an inhibitor of SIRT3, significantly enhanced PA-induced lipid accumulation and oxidative stress in CD38 overexpressing cell lines. In conclusion, our results demonstrated that CD38 deficiency prevented DN by inhibiting lipid accumulation and oxidative stress through activation of the SIRT3 pathway.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141905787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromatin- and nucleosome-associated features in liquid biopsy: implications for cancer biomarker discovery. 液体活检中的染色质和核糖体相关特征：对发现癌症生物标记物的意义

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-08-01 Epub Date: 2024-03-13 DOI: 10.1139/bcb-2024-0004

Lucas Penny, Sasha C Main, Steven D De Michino, Scott V Bratman

引用次数: 0

Antibacterial, anti-invasive, and anti-inflammatory activity of bovine lactoferrin extracted from milk or colostrum versus whole colostrum. 从牛奶或初乳中提取的牛乳铁蛋白与整个初乳相比，具有抗菌、抗侵入和抗炎活性。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-08-01 Epub Date: 2024-05-29 DOI: 10.1139/bcb-2024-0021

Luigi Rosa, Giusi Ianiro, Antonietta Lucia Conte, Maria Pia Conte, Livia Ottolenghi, Piera Valenti, Antimo Cutone

{"title":"Antibacterial, anti-invasive, and anti-inflammatory activity of bovine lactoferrin extracted from milk or colostrum versus whole colostrum.","authors":"Luigi Rosa, Giusi Ianiro, Antonietta Lucia Conte, Maria Pia Conte, Livia Ottolenghi, Piera Valenti, Antimo Cutone","doi":"10.1139/bcb-2024-0021","DOIUrl":"10.1139/bcb-2024-0021","url":null,"abstract":"Lactoferrin (Lf), a multifunctional cationic glycoprotein extracted from milk or colostrum, is able to chelate two ferric ions per molecule, inhibit the formation of reactive oxygen species, interact with the anionic components of bacteria or host cells, and enter inside host cell nucleus, thereby exerting antibacterial, anti-invasive, and anti-inflammatory activities. By virtue of Lf presence, bovine colostrum is expected to perform analogous functions to pure Lf, along with additional activities attributable to other bioactive constituents. The present research aims to compare the antibacterial, anti-invasive, and anti-inflammatory activities of bovine Lf purified from milk (mbLf) and colostrum (cbLf) in comparison to those exhibited by whole bovine colostrum (wbc). The results demonstrated a major efficacy of mbLf in inhibiting pathogenic bacteria and in exerting anti-invasive and anti-survival activities with respect to cbLf and wbc. Furthermore, mbLf lowered IL-6 levels to those of uninfected cells, while a less evident decrease was observed upon cbLf treatment. Conversely, wbc managed to slightly lower IL-6 levels compared to those synthesized by infected cells. These data demonstrate that, to obtain maximum effectiveness in such activities, Lf should be formulated/used without addition of other substances and should be sourced from bovine milk rather than colostrum.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141173993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0