Biochemistry and Cell Biology最新文献

Quercetin alleviates the progression of chronic rhinosinusitis by affecting nasal mucosal epithelial remodeling, inflammation and Treg/Th17 imbalance. 槲皮素通过影响鼻黏膜上皮重塑、炎症和Treg/Th17失衡来缓解慢性鼻窦炎的进展。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-07-02 DOI: 10.1139/bcb-2025-0049

Xiaopeng Qu, Pengyu Tao, Jiajia Dong, Lingzhao Meng

{"title":"Quercetin alleviates the progression of chronic rhinosinusitis by affecting nasal mucosal epithelial remodeling, inflammation and Treg/Th17 imbalance.","authors":"Xiaopeng Qu, Pengyu Tao, Jiajia Dong, Lingzhao Meng","doi":"10.1139/bcb-2025-0049","DOIUrl":"https://doi.org/10.1139/bcb-2025-0049","url":null,"abstract":"Nasal mucosal epithelial tissue remodeling and persist inflammation are related to the development of chronic rhinosinusitis (CRS). Quercetin possesses multiple biological properties in several inflammatory diseases. However, its roles in CRS remain unclear. In this study, serumstaphylococcus aureus enterotoxin B (SEB) increased inflammatory response in human nasal epithelial cells (hNECs), which was reversed by quercetin. Moreover, quercetin inhibited SEB-evoked epithelial-mesenchymal transition (EMT) in hNECs by increasing EMT marker E-cadherin and decreasing N-cadherin expression. Concomitantly, SEB-induced increases in transcripts and release of MMP-9 were reduced by quercetin. Mechanistically, quercetin inhibited SEB-induced activation of the TLR2-NF-kB axis in hNECs. Moreover, restoring TLR2 signaling reversed quercetin-mediated inhibition of SEB-induced inflammation, EMT and MMP-9 expression. In vivo, quercetin attenuated histopathological changes of nasal mucosal tissues in Staphylococcus aureus-constructed CRS mice. Concomitantly, quercetin alleviated inflammatory response and nasal mucosal remodeling by suppressing EMT and MMP-9 levels. Additionally, quercetin ameliorated imbalance of Treg/Th17 proportions. Notably, quercetin suppressed activation of the TLR2-NF-kB axis, while restoring this signaling reversed quercetin-mediated protection against CRS. Thus, quercetin may attenuate pathological progression of CRS by inhibiting nasal mucosal tissue remodeling, inflammation and Treg/Th17 imbalance, which may be associated with inhibition of TLR2-NF-kB axis, supporting a promising therapeutic agent for CRS.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A reliable western blot workflow with improved dynamic range for the detection of myelin proteins in murine brain. 一种可靠的western blot工作流程，具有改进的动态范围，用于检测小鼠大脑中的髓磷脂蛋白。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-06-13 DOI: 10.1139/bcb-2025-0093

Victor P Liu, Shaheer Lakhani, Kendra L Furber

{"title":"A reliable western blot workflow with improved dynamic range for the detection of myelin proteins in murine brain.","authors":"Victor P Liu, Shaheer Lakhani, Kendra L Furber","doi":"10.1139/bcb-2025-0093","DOIUrl":"https://doi.org/10.1139/bcb-2025-0093","url":null,"abstract":"Myelin is a highly structured multilamellar sheath produced by oligodendrocytes, which insulates neuronal axons to facilitate neurotransmission. Maturation of oligodendrocytes in cortical regions of the developing murine brain occurs postnatally and corresponds to the marked upregulation of myelin-specific genes. Western blotting is a conventional technique used to study protein expression but historically has only been considered semi-quantitative. This study aims to optimize a western blot workflow for quantification of myelin proteins in murine brain including the examination of the following parameters: sample preparation, electrophoretic transfer conditions, detection parameters, data normalization and linear dynamic range. As a proof of principle, the optimized protocol was employed to characterize both the absolute and relative expression of myelin oligodendrocyte glycoprotein (MOG) throughout neurodevelopment. A dynamic loading paradigm, which varied total protein load across different age groups to ensure antigen detection remained in the linear dynamic range of the assay, showed a greater relative increase in expression when compared to standard loading paradigm. This approach resulted in comparable MOG expression profiles from both absolute and relative quantification. The optimized western blot workflow will facilitate protein quantification and will improve data reproducibility when investigating the molecular mechanisms of myelination in development, aging and disease.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bovine lactoferrin suppresses the proliferation of endometriotic stromal cells via the PI3K/Akt/mTOR pathway. 牛乳铁蛋白通过PI3K/Akt/mTOR通路抑制子宫内膜异位症基质细胞的增殖。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-06-09 DOI: 10.1139/bcb-2025-0014

Akiko Nakamura, Yuji Tanaka, Shunichiro Tsuji, Tsukuru Amano, Akie Takebayashi, Akimasa Takahashi, Ayako Inatomi, Tetsuro Hanada, Takashi Murakami

{"title":"Bovine lactoferrin suppresses the proliferation of endometriotic stromal cells via the PI3K/Akt/mTOR pathway.","authors":"Akiko Nakamura, Yuji Tanaka, Shunichiro Tsuji, Tsukuru Amano, Akie Takebayashi, Akimasa Takahashi, Ayako Inatomi, Tetsuro Hanada, Takashi Murakami","doi":"10.1139/bcb-2025-0014","DOIUrl":"https://doi.org/10.1139/bcb-2025-0014","url":null,"abstract":"The most common medical therapy for endometriosis suppresses ovulation, which is a barrier for patients planning pregnancy. To address this issue, we focused on the cell proliferation-suppressing effects of lactoferrin, which reportedly in various malignant tumours. Despite being a benign disease, endometriotic cells have similar characteristics to malignant tumours, which may be involved in its onset and progression. Endometriotic and endometrial stromal cells were obtained from patients with endometriosis. After culture with 1 mg/mL of bovine lactoferrin, cell proliferation was significantly suppressed in endometriotic stromal cells compared to controls, but this remained unchanged in endometrial stromal cells. Bovine lactoferrin also significantly increased the number of endometriotic stromal cells in the G0/G1 phase and significantly decreased those in the S phase, and suppressed the protein expression of phosphorylated-AKT, phosphorylated-mTOR, phosphorylated-S6K, and cyclin D1. Bovine lactoferrin inhibits the transition from the G1 to the S phase by suppressing the PI3K/Akt/mTOR pathway and reducing the synthesis of cyclin D1, thereby arresting the cell cycle at the G1 phase. Bovine lactoferrin suppressed the proliferation of endometriotic stromal cells without suppressing the proliferation of endometrial stromal cells. Lactoferrin, which allows for pregnancy and lactation during administration, has potential as a novel therapeutic candidate for endometriosis.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MCPIP1 deficiency alleviates abdominal aortic aneurysm formation by inhibiting MAPK signaling. MCPIP1缺失通过抑制MAPK信号通路减轻腹主动脉瘤的形成。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-06-06 DOI: 10.1139/bcb-2024-0260

Ming Xue, Hailing Ding, Yongxin Han, Yuhua Wei, Xuming Wang, Xiaohan Wang, Xiangqian Kong

{"title":"MCPIP1 deficiency alleviates abdominal aortic aneurysm formation by inhibiting MAPK signaling.","authors":"Ming Xue, Hailing Ding, Yongxin Han, Yuhua Wei, Xuming Wang, Xiaohan Wang, Xiangqian Kong","doi":"10.1139/bcb-2024-0260","DOIUrl":"https://doi.org/10.1139/bcb-2024-0260","url":null,"abstract":"Abdominal aortic aneurysm (AAA) is a chronic and severe aortic disease. Our previous studies have indicated that monocyte chemotactic protein-induced protein-1 (MCPIP1) is involved in AAA. However, the exact effect of MCPIP1 on angiotensin II (Ang II)-induced AAA formation is currently unknown. MCPIP1 deficiency reduced AAA formation in Ang II-induced mice. Less collagen and elastin degradation were observed in MCPIP1 deficient mice treated with Ang II. Ang II decreased αSMA and SM22α levels in aortas and VSMCs, whereas MCPIP1 deficiency reduced this decrease. MCPIP1 deficiency also attenuated Ang II-induced expression of MAPK signaling-associated proteins in aortas and VSMCs. Silencing MCPIP1 decreased proliferation and migration in Ang II-induced VSMCs. Furthermore, inactivation of ERK1/2 with PD98059 reduced Ang II-induced proliferation and migration of VSMCs. Dual luciferase and chromatin immunoprecipitation assay results confirmed that MCPIP1 was transcriptionally regulated by KLF4. KLF4 knockdown reversed the facilitating effect of Ang II on MCPIP1 expression. In conclusion, our findings suggest that MCPIP1 promotes Ang II-induced VSMCs phenotypic switching via the MAPK signaling pathway.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subacute exposure to aluminium chloride induces cytotoxicity and oxidative stress in rat erythrocytes: A dose-dependent study. 亚急性暴露于氯化铝诱导大鼠红细胞细胞毒性和氧化应激：剂量依赖性研究。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-05-30 DOI: 10.1139/bcb-2024-0236

Farha Shahabuddin, Samina Naseem, Farah Khan

{"title":"Subacute exposure to aluminium chloride induces cytotoxicity and oxidative stress in rat erythrocytes: A dose-dependent study.","authors":"Farha Shahabuddin, Samina Naseem, Farah Khan","doi":"10.1139/bcb-2024-0236","DOIUrl":"https://doi.org/10.1139/bcb-2024-0236","url":null,"abstract":"Aluminium (Al) toxicity has attracted considerable interest due to its bioavailability, environmental persistence, and adverse health effects. The present study investigates the effect of Al on rat erythrocytes under in vivo conditions. Rats were administered 0 (control), 25 (Al 1), 35 (Al 2), 45 (Al 3), and 55 (Al 4) mg/kg b.wt of AlCl3 orally for 30 days. Hemolysates were prepared from different experimental groups and assayed for various biochemical parameters. AlCl₃ administration significantly increased protein oxidation and lipid peroxidation, while decreasing total sulfhydryl and glutathione levels in rat erythrocytes. Methemoglobin level was increased and methemoglobin reductase activity was decreased upon AlCl3 treatment. Prolonged AlCl3 exposure inhibited the activities of antioxidant enzymes, and lowered the cells' antioxidant power. It also caused an increase in H2O2 and NO levels showing generation of oxidative and nitrosative stress. AlCl3 intoxication adversely affected the membrane-bound and metabolic enzyme activities. Alterations in all the biochemical parameters were found in an AlCl3 dose-dependent manner. Scanning electron microscopy showed gross morphological changes in erythrocytes, from discocytes to acanthocytes and echinocytes, further supporting the damaging effect of aluminium. The aluminium-induced oxidative stress seems to be the key mechanism of damage to the cellular components that could lead to red cell senescence.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A combinatorial multi-site directed mutagenesis solution for improved thermal stability of Lactobacillus plantarum tannase. 提高植物乳杆菌单宁酶热稳定性的组合多位点定向诱变溶液。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-05-29 DOI: 10.1139/bcb-2025-0134

T Deandre Chevannes, Antony D St-Jacques, Matthew Eric Loewen, Michele C Loewen

{"title":"A combinatorial multi-site directed mutagenesis solution for improved thermal stability of Lactobacillus plantarum tannase.","authors":"T Deandre Chevannes, Antony D St-Jacques, Matthew Eric Loewen, Michele C Loewen","doi":"10.1139/bcb-2025-0134","DOIUrl":"https://doi.org/10.1139/bcb-2025-0134","url":null,"abstract":"This study used a modified flapless (FLT) version of tannase from Lactobacillus plantarum, (LpTan) to explore the effects of 'stacking' site mutations predicted by Protein Repair One Stop Shop (PROSS) to increase stability. Four different LpTan structural-state models (including apo, substrate- and product- bound as well as FLT) were comparatively applied, yielding 143 predicted mutations. Of these, 8 mutations (including Q63T, A65D, A184Y, A257D, V276Y, T321G, G421D and G439D (FLT numbering)) were selected to stack, based on conservation of the prediction across all four structural states. Combinatorial screening of the arising 256-member library yielded a selection of possible hits, of which four were further characterized. Variant P6H7 contained 7 of the 8 mutations (excluding V276Y) and showed the highest significant kcat, 17 % higher than FLT and 30 % higher than LpTan, and a 4.5 °C increase in Tm. Variant P8E5 with 6 of 8 mutations (excluding A257D and G439D), yielded a 6.5 °C increase in Tm compared to FLT. The two other variants showed more moderate increases, albeit still greater than FLT or LpTan. Overall, the ability to design thermal stabilized versions of a tannase is emphasized. Putative mechanisms underlying the stabilization imparted by the highlighted variations are discussed.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ophiopogonin D protects against cerebral ischemia-reperfusion injury in rats by inhibiting STAT3 phosphorylation. 麦冬皂苷D通过抑制STAT3磷酸化对大鼠脑缺血再灌注损伤有保护作用。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-05-27 DOI: 10.1139/bcb-2024-0328

Zheng Tang, Chunmei Liu

{"title":"Ophiopogonin D protects against cerebral ischemia-reperfusion injury in rats by inhibiting STAT3 phosphorylation.","authors":"Zheng Tang, Chunmei Liu","doi":"10.1139/bcb-2024-0328","DOIUrl":"https://doi.org/10.1139/bcb-2024-0328","url":null,"abstract":"Our aim is to explore the protective effect of ophiopogonin D (OPD) on cerebral ischemia/reperfusion injury (CIRI) and its relevant molecular mechanisms. OPD effect on brain injury of CIRI rats was evaluated using 2,3,5-triphenyltetrazolium chloride staining, neurological deficit score, brain water content, hematoxylin and eosin staining, Nissl staining and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labelling staining. Immunofluorescence, immunohistochemistry, qRT-PCR, western blot and relative kits were used for detecting inflammatory factors, oxidative stress related factors, and mRNA and protein levels. The Cell Counting Kit-8 assay and flow cytometry were applied for assessing the viability and apoptosis of oxygen-glucose deprivation)/reoxygenation (OGD/R)-induced PC12 cells. OPD ameliorated brain injury via inhibiting neurons apoptosis, oxidative stress and inflammatory response in cerebral infarction rats. In addition, we found that OPD attenuated the apoptosis, oxidative stress and inflammatory response in OGD/R-induced PC12 cells. Both in CIRI rats and OGD/R-induced PC12 cells, OPD was demonstrated to inhibit signal transducer and activator of transcription 3 (STAT3) phosphorylation. Moreover, Colivelin TFA (a STAT3 activator) reversed OPD effect on the apoptosis, oxidative stress and inflammatory response in OGD/R-induced PC12 cells. Our findings demonstrated that OPD could protect against CIRI in rats by inhibiting STAT3 phosphorylation.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An artifact of recombinatorial cloning challenges established beliefs of plasmid cotransformation, selection, and maintenance. 重组克隆的产物挑战了质粒共转化、选择和维持的既定信念。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-05-27 DOI: 10.1139/bcb-2025-0096

Courtney L Geer, Michael Charette

引用次数: 0

Origin of dedifferentiated adipocyte-derived cells (DFAT) during ceiling culture in an Adiponectin Cre-Recombinase mouse model. 在脂肪蛋白 Cre 重组酶小鼠模型的上限培养过程中，已分化脂肪细胞衍生细胞（DFAT）的起源。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 Epub Date: 2024-10-30 DOI: 10.1139/bcb-2024-0140

Marie-Frédérique Gauthier, Giada Ostinelli, Mélissa Pelletier, André Tchernof

{"title":"Origin of dedifferentiated adipocyte-derived cells (DFAT) during ceiling culture in an Adiponectin Cre-Recombinase mouse model.","authors":"Marie-Frédérique Gauthier, Giada Ostinelli, Mélissa Pelletier, André Tchernof","doi":"10.1139/bcb-2024-0140","DOIUrl":"10.1139/bcb-2024-0140","url":null,"abstract":"DFAT cells represent an attractive source of stem cells in tissue engineering and in the potential treatment of several clinical conditions. Our objective was to determine whether DFAT cells originate from mature adipocytes and address whether contamination from the stromal vascular fraction (SVF) could be as a source for these cells. A murine adiponectin-creERT;mT/mG model was used with the excision of the cassette induced by tamoxifen injection for the cells expressing adiponectin (adipoq). This model allows distinguishing of mature adipocytes (green fluorescence) from other SVF cell types (red fluorescence) based on the fluorescent protein expressed. Mature adipocytes and SVF cells were isolated from adipose tissues by collagenase digestion. Ceiling cultures were imaged by time-lapse microscopy. Confocal microscopy was used to follow cells over 21 days. Time-lapse microscopy experiments showed liposecretion occurring in mature adipocytes displaying green fluorescence. Confocal imaging allowed the identification of a heterogeneous cell population expressing green but also red fluorescence after 21 days of culture. Asymmetrical division of mature adipocytes was not observed. In conclusion, liposecretion of mature adipocytes is a phenomenon that can be observed in vitro and DFAT cells do originate from mature adipocytes. However, the population of DFAT cells is heterogenous.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-10"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

1-Deoxynojirimycin affects high glucose-induced pancreatic beta-cell dysfunction through regulating CEBPA expression and AMPK pathway. 1-脱氧野尻霉素通过调节CEBPA表达和AMPK通路影响高血糖诱导的胰岛β细胞功能障碍

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 Epub Date: 2024-11-15 DOI: 10.1139/bcb-2024-0128

Xiaoying Li, Shenggui Liu, Siqi Wang, Xinghui Ai, Lin Wei

引用次数: 0