Biochemistry and Cell Biology最新文献

An optimized protocol for identifying S-acylated proteins in Dictyostelium discoideum. 一种鉴定盘状盘齿龙s -酰化蛋白的优化方案。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-05-06 DOI: 10.1139/bcb-2026-0052

Samer A Owiar, William David Kim, Robert Joseph Huber

引用次数: 0

Caspase Non-canonical Roles in Mediating Lipid Homeostasis. 半胱天冬酶在调节脂质稳态中的非规范作用。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-05-06 DOI: 10.1139/bcb-2026-0061

Fan Du, Maotian Wang, Siyu Ji

{"title":"Caspase Non-canonical Roles in Mediating Lipid Homeostasis.","authors":"Fan Du, Maotian Wang, Siyu Ji","doi":"10.1139/bcb-2026-0061","DOIUrl":"https://doi.org/10.1139/bcb-2026-0061","url":null,"abstract":"Caspases are well recognized and studied as the central executioners of apoptosis, while recent studies have unraveled their diverse roles beyond cell apoptotic function.One emerging area of interest is non-apoptotic caspase activity as a potential regulator in lipid metabolism. This review explores the differential non-apoptotic role of caspases in lipid metabolic process, encompassing the influence of caspases on lipid reprogramming, lipid synthesis, degradation, transport, and signaling. We discuss the mechanisms underlying these interactions, their relevance to physiological processes, and their implications for various diseases. This reveals a hidden layer of regulation where the machinery of cell death is repurposed to control the fundamental processes of fat handling in living animal. Dysregulation of this system forms a vicious cycle that propagates cellular dysfunction, directly contributing to the pathogenesis of major human diseases in aging, including redox stress, NASH, type 2 diabetes, neurodegenerative disorders, and cancer. Consequently, the caspase-metabolism axis emerges as a compelling therapeutic frontier. However, the clinical translation of caspase modulators is challenged by the context-dependent duality of caspase functions.Future therapeutic strategies must therefore advance beyond pan-inhibition toward the precise, context-specific modulation of discrete caspase-mediated metabolic events to halt disease progression effectively.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Metaboloepigenetic Variability in Bovine Fibroblast Lines. 探讨牛成纤维细胞系的代谢表观遗传变异性。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-05-04 DOI: 10.1139/bcb-2025-0438

Eric Hawrylyshyn, Marcia Ferraz, Biankha Chan, Gabriela Mastromonaco

{"title":"Exploring Metaboloepigenetic Variability in Bovine Fibroblast Lines.","authors":"Eric Hawrylyshyn, Marcia Ferraz, Biankha Chan, Gabriela Mastromonaco","doi":"10.1139/bcb-2025-0438","DOIUrl":"https://doi.org/10.1139/bcb-2025-0438","url":null,"abstract":"Somatic cell-based reproductive technologies (SCRTs) offer a promising approach for preserving the genetic diversity of threatened species. SCRTs require the nuclear reprogramming of a donor somatic cell, which has low success rates (1-5%) and can vary significantly between individuals and cell lines derived from the same individual. Somatic and pluripotent cells differ in several key characteristics, and donor cells presenting more pluripotent-like traits have been found to result in improved reprogramming outcomes. Here, we characterized 18 standardized fibroblast cell lines from six Angus bulls for several of these characteristics at the inter- and intra-individual levels: bioenergetic status, targeted metabolite abundance, global DNA methylation levels, and presence of key histone modifications. Differences in the metaboloepigenetic profiles of these cell lines were observed at both inter- and intra-individual levels. By integrating these data, we positioned cell lines along a somatic-to-pluripotent-like continuum and identified a reduced set of features that captured the dominant separation between profiles. Although functional reprogramming outcomes were not assessed here, these results provide a data-driven framework to prioritise donor cell lines for prospective iPSC/SCNT validation and to guide the development of practical biomarker panels for SCRT workflows.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Terpenes as Modulators of Nociceptive Signaling: Behavioral and Molecular Insights from Caenorhabditis elegans. 萜烯作为伤害性信号的调节剂：秀丽隐杆线虫的行为和分子观察。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-04-29 DOI: 10.1139/bcb-2025-0380

Kaoutar Benkhraba, Fatma Boujenoui, Jesus David Castaño, Jabin Sultana, Francis Beaudry

{"title":"Terpenes as Modulators of Nociceptive Signaling: Behavioral and Molecular Insights from Caenorhabditis elegans.","authors":"Kaoutar Benkhraba, Fatma Boujenoui, Jesus David Castaño, Jabin Sultana, Francis Beaudry","doi":"10.1139/bcb-2025-0380","DOIUrl":"https://doi.org/10.1139/bcb-2025-0380","url":null,"abstract":"Terpenes such as Limonene (LIMO) and β-Caryophyllene (BC) have demonstrated pain-modulating properties, potentially through interactions with the TRPV1 receptor. This study examines the antinociceptive effects of four terpenes derived from Cannabis sativa: Limonene, β-Caryophyllene, α-Humulene (HUM), and α-Myrcene (MYR) using Caenorhabditis elegans (C. elegans). The objective was to characterize terpene-induced modulation of nocifensive responses to noxious heat, and to elucidate their influence on molecular pathways via specific receptor targets. Thermotaxis assays quantified the antinociceptive activity of increasing terpene concentrations in wild-type nematodes. To assess receptor-specific mechanisms, assays were performed in mutant strains lacking functional OCR-2 and OSM-9 (TRPV-like nociceptors), and NPR-19 and NPR-32 (encoding cannabinoid-like receptors). Proteomic profiling coupled with bioinformatics analysis identified terpene-induced alterations in signaling pathways and biological processes. All four terpenes exhibited significant antinociceptive activity in wild-type C. elegans, with impaired effects observed in vanilloid receptor mutants, implicating TRPV-like channels in their mechanism of action. Proteomic and pathway analyses revealed terpene-specific molecular signatures, highlighting differential modulation of neuronal and stress-responsive signaling cascades. By elucidating the molecular mechanisms underlying terpene-induced nociceptive modulation, this work strengthens the growing body of evidence supporting the therapeutic promise of terpenes in pain management outside the effect referred to as the \"entourage effect.\"","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE UNIVERSALLY CONSERVED NTPASE OLA1. 普遍保守的ntpase ola1。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-04-16 DOI: 10.1139/bcb-2025-0347

Jessica Semmelrock, Hans-Joachim Wieden

{"title":"THE UNIVERSALLY CONSERVED NTPASE OLA1.","authors":"Jessica Semmelrock, Hans-Joachim Wieden","doi":"10.1139/bcb-2025-0347","DOIUrl":"https://doi.org/10.1139/bcb-2025-0347","url":null,"abstract":"The non-canonical translation factor OBG-like ATPase 1 (OLA1) is conserved across all domains of life. OLA1 has been studied for decades by many groups, often under different names such GBP45, GTBP9, DOC45, PTD004, EngD, YyaF, Ybr025c, and in bacteria, YchF. Studies have correlated OLA1 with iron metabolism, oxidative stress response, mediation of temperature stress, and virulence. OLA1 has gained attention for its implicated role in a wide range of health conditions and cellular processes including cancer, centrosome regulation/cell proliferation, heart disease, persistent pulmonary hypertension of the newborn, mitochondrial function, neuronal differentiation, protein degradation, atherosclerosis, Down's Syndrome, as well as ribosome-dependent protein synthesis. On the background of such a wide range of functional implications, insight into the molecular mechanism of the evolutionary ancient OLA1 will help to explain the breadth of phenotypic traits. Information about the different protein structural domains present in OLA1 and results fromOLA1 in vivo and in vitro studies suggest a role in ribosome dependent translation regulation based on molecular mimicry with the canonical translation factors.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147697471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

USP54 regulates the malignancy and autophagy in nasopharyngeal cancer cells by modulating ULK1 ubiquitination levels. USP54通过调节ULK1泛素化水平调控鼻咽癌细胞的恶性和自噬。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2025-0235

Fanrui Fu, Yong Wu, Peilin Wang, Wubin Yuan, Yi Jiang

{"title":"USP54 regulates the malignancy and autophagy in nasopharyngeal cancer cells by modulating ULK1 ubiquitination levels.","authors":"Fanrui Fu, Yong Wu, Peilin Wang, Wubin Yuan, Yi Jiang","doi":"10.1139/bcb-2025-0235","DOIUrl":"10.1139/bcb-2025-0235","url":null,"abstract":"Nasopharyngeal carcinoma (NPC) is an aggressive malignancy with poor outcomes at advanced stages. Although ubiquitination is critical in regulating oncogenic pathways, the role of many ubiquitin-specific proteases (USPs) in NPC remains unexplored. In this study, we conducted a systematic screen of USPs to identify regulators of ULK1, a key initiator of autophagy and oncogene in NPC. We identified USP54 as a potent stabilizer of ULK1. Bioinformatic analyses of NPC transcriptomic datasets revealed that both ULK1 and USP54 are significantly overexpressed in NPC tissues. Functional assays demonstrated that USP54 overexpression enhanced NPC cell viability, colony formation, migration, and invasion by promoting autophagy, independently of PI3K/AKT pathway activity. Importantly, pharmacological inhibition of autophagy or genetic silencing of ULK1 abolished the oncogenic effects of USP54, indicating that ULK1-dependent autophagy mediates USP54-driven NPC progression. Our findings revealed that USP54 may promote autophagy and malignancy in NPC via regulating ULK1 stability, highlighting USP54 as a potential therapeutic target for NPC treatment.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-12"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145740750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An improved version of the early histone HCl extraction protocol. 早期组蛋白HCl提取方案的改进版本。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2025-0335

Geneveve Analike, Dhanvi Prajapati, James R Davie, Juan Ausió

引用次数: 0

SOX2 transcriptional regulation by enhancer elements: a comprehensive review with a unified enhancer nomenclature. Sox2转录调控的增强子元件：一个全面的审查与统一的增强子命名。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2025-0375

Mariia Cherednychenko, Natalia A Gajewska, Jennifer A Mitchell

{"title":"SOX2 transcriptional regulation by enhancer elements: a comprehensive review with a unified enhancer nomenclature.","authors":"Mariia Cherednychenko, Natalia A Gajewska, Jennifer A Mitchell","doi":"10.1139/bcb-2025-0375","DOIUrl":"10.1139/bcb-2025-0375","url":null,"abstract":"Transcription factor SOX2 is essential for a number of biological processes, including mammalian nervous system development and adult brain stem cell maintenance. Expression of this gene requires precise control by a complex network of regulatory elements, which still remains poorly understood. Years of research by different groups have generated a large amount of data on multiple SOX2 enhancers, with varying levels of evidence for their activity across species and tissues. However, the volume of information in the field and inconsistent nomenclature, with the same enhancer referred to by different study-specific names, make understanding progress in SOX2 enhancer regulation challenging. In this review, we brought together current knowledge on predicted and experimentally validated SOX2 enhancers, highlighting links between conserved elements studied in different species. We also propose a unified enhancer naming system based on the distance from the SOX2 transcription start site in the genome of interest, aiming to improve consistency and make communication in the field more straightforward.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-15"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146096757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chelerythrine alleviates inflammation and angiogenesis in a mouse rosacea model via suppressing the NF-κB/p38 MAPK/STAT3 pathways. 车车草碱通过抑制NF-κB/p38 MAPK/STAT3通路减轻小鼠酒痤疮模型的炎症和血管生成。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2025-0124

Rong Zhou, Zhibo Yang, Junwen Wang, Chang Wang, Meijunzi Luo, Yi Pan, Pan Huang, Yi-Ning Yan, Di Long, Haizhen Wang

{"title":"Chelerythrine alleviates inflammation and angiogenesis in a mouse rosacea model via suppressing the NF-κB/p38 MAPK/STAT3 pathways.","authors":"Rong Zhou, Zhibo Yang, Junwen Wang, Chang Wang, Meijunzi Luo, Yi Pan, Pan Huang, Yi-Ning Yan, Di Long, Haizhen Wang","doi":"10.1139/bcb-2025-0124","DOIUrl":"10.1139/bcb-2025-0124","url":null,"abstract":"Rosacea is a chronic inflammatory skin condition marked by excessive M1 macrophage polarization and angiogenesis, resulting in erythema and tissue inflammation. Despite available treatments, many patients experience recurrent flare-ups. This study explores chelerythrine, a bioactive component of Phellodendri Chinensis Cortex, for its therapeutic potential in rosacea through modulation of NF-κB, p38 MAPK, and STAT3 signaling, inflammation, and vascular regulation. Using an LL-37-induced rosacea-like mouse model, THP-1-derived M1 macrophages and human umbilical vein endothelial cells (HUVECs), chelerythrine's effects on macrophage polarization, cytokine expression, angiogenesis, and pathway activation of NF-κB, p38 MAPK, and STAT3 were evaluated. Chelerythrine significantly reduced epidermal thickness, inflammatory cell infiltration, and pro-inflammatory markers (TNF-α and IL-1β). It inhibited NF-κB, p38 MAPK, and STAT3 activation and decreased M1 polarization markers, shifting towards an anti-inflammatory profile. Furthermore, chelerythrine reduced vascular density and VEGF expression, impairing angiogenesis-related behaviors in HUVECs. These findings suggest that chelerythrine holds promise as a treatment for rosacea by mitigating inflammation and angiogenesis through targeted multiple pathways and macrophage modulation.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-12"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural determinants for the monomeric and phospholipid-transport P4B-ATPase via comprehensive in silico analysis of P4-ATPase structures. 通过对p4 - atp酶结构的全面硅分析，单体和磷脂转运p4b - atp酶的结构决定因素。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2025-0324

Kadambari Vijay Sai, Danny Farhat, Sai Anvithaa Sundar Rajan, Jyh-Yeuan Lee

{"title":"Structural determinants for the monomeric and phospholipid-transport P4B-ATPase via comprehensive in silico analysis of P4-ATPase structures.","authors":"Kadambari Vijay Sai, Danny Farhat, Sai Anvithaa Sundar Rajan, Jyh-Yeuan Lee","doi":"10.1139/bcb-2025-0324","DOIUrl":"10.1139/bcb-2025-0324","url":null,"abstract":"The P4-ATPase family of phospholipid flippases plays a critical role in maintenance of membrane asymmetry and cellular protein traffic and eukaryotic homeostasis. Several structures of these phospholipid flippases have been resolved, along with extensive biochemical characterization of the substrate transport properties. However, an essential subfamily of monomeric phospholipid flippases, the P4B-ATPases, remains to be characterized in depth. While P4B-ATPases appear to share similar lipid transport properties to their heterodimeric counterparts, the P4A-ATPases, the basis of their substrate translocation as monomers is currently unknown. In this study, we investigated the divergence of P4B-ATPases from other P-type ATPases using a structure-based analysis of sequence conservation. Our results showed conservative and nonconservative pockets and pathways in the P4B-ATPases near critical residues for the substrate transport pathway. P4B-ATPases also exhibit a unique interaction of an invariant proline near a conserved TM1-2 aromatic cluster, where dynamics simulation confirmed interactions with phospholipids and cholesterol by this conserved aromatic cluster. A critical TM6 residue was observed orienting into a conserved P4B-pathway whose function is currently unknown but a disease mutation hotspot, suggesting that P4B-ATPases exhibit novel transport and/or regulatory mechanisms. Our results provide a molecular framework for further studies on this essential subfamily of monomeric phospholipid flippases.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-11"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0