Biochemistry and Cell Biology最新文献

Origin of dedifferentiated adipocyte-derived cells (DFAT) during ceiling culture in an Adiponectin Cre-Recombinase mouse model. 在脂肪蛋白 Cre 重组酶小鼠模型的上限培养过程中，已分化脂肪细胞衍生细胞（DFAT）的起源。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-10-30 DOI: 10.1139/bcb-2024-0140

Marie-Frédérique Gauthier, Giada Ostinelli, Mélissa Pelletier, Andre Tchernof

{"title":"Origin of dedifferentiated adipocyte-derived cells (DFAT) during ceiling culture in an Adiponectin Cre-Recombinase mouse model.","authors":"Marie-Frédérique Gauthier, Giada Ostinelli, Mélissa Pelletier, Andre Tchernof","doi":"10.1139/bcb-2024-0140","DOIUrl":"https://doi.org/10.1139/bcb-2024-0140","url":null,"abstract":"Dedifferentiated adipose tissue-derived (DFAT) cells represent an attractive source of stem cells for tissue engineering and the potential treatment of several clinical conditions. Our objective was to determine whether DFAT cells originate from mature adipocytes and address whether contamination from the stromal vascular fraction (SVF) could be as a source for these cells. A murine adiponectin-creERT; mT/mG model was used with the excision of the cassette induced by tamoxifen injection for the cells expressing adiponectin (adipoq). This model allows distinguishing mature adipocytes (green fluorescence) from other SVF cell types (red fluorescence) based on the fluorescent protein expressed. Mature adipocytes and SVF cells were isolated from adipose tissues by collagenase digestion. Ceiling cultures were imaged by time-lapse microscopy. Confocal microscopy was used to follow cells over 21 days. Time-lapse microscopy experiments showed liposecretion occurring in mature adipocytes displaying green fluorescence. Confocal imaging allowed the identification of a heterogeneous cell population expressing green but also red fluorescence after 21 days of culture. Asymmetrical division of mature adipocytes was not observed. In conclusion, liposecretion of mature adipocytes is a phenomenon that can be observed in vitro and DFAT cells do originate from mature adipocytes. However, the population of DFAT cells is heterogenous.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fusarium graminearum Ste2 and Ste3 Receptors Undergo Peroxidase-Induced Heterodimerization when Expressed Heterologously in Saccharomyces cerevisiae. 禾本科镰刀菌 Ste2 和 Ste3 受体在酿酒酵母中异源表达时发生过氧化物酶诱导的异源二聚化。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-10-22 DOI: 10.1139/bcb-2024-0104

Tanya Sharma, Robert Y Jomphe, Dongling Zhang, Ana C Magalhaes, Michele C Loewen

{"title":"Fusarium graminearum Ste2 and Ste3 Receptors Undergo Peroxidase-Induced Heterodimerization when Expressed Heterologously in Saccharomyces cerevisiae.","authors":"Tanya Sharma, Robert Y Jomphe, Dongling Zhang, Ana C Magalhaes, Michele C Loewen","doi":"10.1139/bcb-2024-0104","DOIUrl":"https://doi.org/10.1139/bcb-2024-0104","url":null,"abstract":"Fusarium graminearum FgSte2 and FgSte3 are G-protein coupled receptors (GPCRs) shown to play roles in hyphal chemotropism and fungal plant pathogenesis in response to activity arising from host-secreted peroxidases. Here, we follow up on the observation that chemotropism is dependent on both FgSte2 and FgSte3 being present; testing the possibility that this might be due to formation of an FgSte2-FgSte3 heterodimer. Bioluminescence resonance energy transfer (BRET) analyses were conducted in Saccharomyces cerevisiae, where the addition of horse radish peroxidase (HRP) was found to increase the transfer of energy from the inducibly-expressed FgSte3-Nano luciferase donor, to the constitutively-expressed FgSte2-yellow fluorescent protein (YFP) acceptor, compared to controls. A partial response was also detected when an HRP-derived ligand-containing extract was enriched from F. graminearum spores and applied instead of HRP. In contrast, substitution with pheromones or an unrelated bovine GPCR, rhodopsin-YFP used as acceptor, eliminated all BRET responses. Interaction results were validated by affinity pulldown and receptor expression was validated by confocal immunofluorescence microscopy. Taken together these findings demonstrate the formation of HRP and HRP-derived ligand stimulated heterodimers between FgSte2 and FgSte3. Outcomes are discussed from the context of the roles of ligands and reactive oxygen species in GPCR dimerization.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiofilm activities of lactoferricin-related Trp- and Arg-rich antimicrobial hexapeptides against pathogenic Staphylococcus aureus and Pseudomonas aeruginosa strains. 乳铁蛋白相关的富含 Trp 和 Arg 的抗菌六肽对致病性金黄色葡萄球菌和铜绿假单胞菌菌株的抗生物膜活性。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-10-17 DOI: 10.1139/bcb-2024-0183

Gopal Ramamourthy, Hans J Vogel

{"title":"Antibiofilm activities of lactoferricin-related Trp- and Arg-rich antimicrobial hexapeptides against pathogenic Staphylococcus aureus and Pseudomonas aeruginosa strains.","authors":"Gopal Ramamourthy, Hans J Vogel","doi":"10.1139/bcb-2024-0183","DOIUrl":"https://doi.org/10.1139/bcb-2024-0183","url":null,"abstract":"Recently, several antimicrobial peptides (AMPs) varying in length from 12 to 37 residues, have been shown to act as antibiofilm agents. Here we report a study of twenty-three hexapeptides modeled after four different Trp- and Arg-rich AMPs, including the RRWQWR-NH2 peptide, derived from bovine lactoferrin. They were tested against the pathogenic Gram-negative Pseudomonas aeruginosa PAO1 strain and a Gram-positive Staphylococcus aureus MRSA strain. Both strains were engineered to express the GFP protein, and fluorescence detection was used to measure the ability of the peptides to prevent biofilm formation (MBIC) or to cause the breakdown of established biofilms (MBEC). Similar antibiofilm activities were obtained with the standard crystal violet dye assay. Most Trp- and Arg-rich hexapeptides displayed a potent antibiofilm activity against the Gram-positive S. aureus MRSA strain. In particular, hexapeptides with 3 Arg and 3 Trp were very effective, especially when they contained the three Trp in sequence. Somewhat unexpectedly, the antimicrobial (MIC) values correlated with the MBIC and MBEC values, which has not been seen for some other AMP/antibiofilm peptides. Our results demonstrate that short Trp- and Arg-rich peptides merit further studies as antibiofilm agents, that could be deployed to address part of the antimicrobial resistance problem.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Treatment of lactoferrin and antimicrobial peptide N6 on bacterial enteritis caused by Escherichia coli in mice. 乳铁蛋白和抗菌肽 N6 对大肠杆菌引起的小鼠细菌性肠炎的治疗作用。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-10-11 DOI: 10.1139/bcb-2024-0103

Xuanxuan Ma, Kun Zhang, Na Yang, Ya Hao, Ruoyu Mao, Da Teng, Jianhua Wang

{"title":"Treatment of lactoferrin and antimicrobial peptide N6 on bacterial enteritis caused by Escherichia coli in mice.","authors":"Xuanxuan Ma, Kun Zhang, Na Yang, Ya Hao, Ruoyu Mao, Da Teng, Jianhua Wang","doi":"10.1139/bcb-2024-0103","DOIUrl":"10.1139/bcb-2024-0103","url":null,"abstract":"Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhea in humans and animals. The study aimed to evaluate the efficacy of bovine lactoferrin (bLf) as an adjuvant combined with AMP (N6) in the treatment of E. coli-induced bacterial enteritis. Firstly, 40 female ICR mice were randomly divided into four groups. The ETEC-A, ETEC-B, and ETEC-C groups were gavaged with 0.2 mL of ETEC K88 at 5 × 109, 5 × 108, and 5 × 107 CFU/mL for three consecutive days, respectively, the CK control group was given PBS. Based on the clinical symptoms and intestinal changes, the optimal model dose of ETEC K88 was determined to be 5 × 108 CFU/mL. Sixty female ICR mice were randomly divided into six groups: CK group (uninfected), NC group (infected and untreated), N6 treatment group (20 mg/kg), bLf treatment group (100 mg/kg), bLf + N6-A treatment group (10 mg/kg N6+100 mg/kg bLf), and bLf + N6-B group (20 mg/kg N6+100 mg/kg bLf). The clinical symptoms, intestinal morphology, inflammatory response and serum metabolites were monitored. The results showed that compared with the NC group, the bLf-N6-A and bLf-N6-B treatment groups had significant reductions in TNF-α and IL-6, significant increases in IL-10, and significant reductions in endotoxin and DAO in plasma (p < 0.05). Meanwhile, the bLf-N6-A and bLf-N6-B treatment groups significantly increased the expression of ZO-1, claudin-1 and occludin, increased the height of small intestinal mucosal villi and VH/CD after ETEC K88-induced intestinal injury (p < 0.05). The combination of bLf and N6 relieved enteritis by balancing intestinal mucosal immunity, improving intestinal morphology and barrier function. BLf combined with N6 can be used as an effective therapeutic strategy for the treatment of bacterial enteritis.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142405978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactoferrin modulates oxidative stress and inflammatory cytokines in a murine model of dysbiosis induced by clindamycin. 在克林霉素诱导的菌群失调小鼠模型中，乳铁蛋白可调节氧化应激和炎症细胞因子。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-10-08 DOI: 10.1139/bcb-2024-0087

Inés Abad, Andrea Bellés, Ana Rodríguez-Largo, Lluís Luján, Ignacio de Blas, Dimitra Graikini, Laura Grasa, Lourdes Sánchez

{"title":"Lactoferrin modulates oxidative stress and inflammatory cytokines in a murine model of dysbiosis induced by clindamycin.","authors":"Inés Abad, Andrea Bellés, Ana Rodríguez-Largo, Lluís Luján, Ignacio de Blas, Dimitra Graikini, Laura Grasa, Lourdes Sánchez","doi":"10.1139/bcb-2024-0087","DOIUrl":"https://doi.org/10.1139/bcb-2024-0087","url":null,"abstract":"Antibiotics, specifically clindamycin, cause intestinal dysbiosis, reducing the microbiota with anti-inflammatory properties. Furthermore, clindamycin can induce alterations in the immune responses and oxidative stress. Lactoferrin, among other activities, participates in the maintenance of intestinal homeostasis and reduces dysbiosis induced by antibiotic treatment. The aim of this study was to analyze the effect of native and iron-saturated bovine LF in a murine model of dysbiosis induced by clindamycin. Six groups of male C57BL/6 mice were treated with saline (control), clindamycin (Clin), native lactoferrin (nLF), iron-saturated lactoferrin (sLF), nLF/Clin or sLF/Clin. Oxidation caused in the intestinal cells of the ileum of animals subjected to different treatments was analyzed, focusing on lipid peroxidation and protein carbonyl content. The expression of inflammatory mediators was determined by qRT-PCR. Treatment with clindamycin did not modify lipid peroxidation, but significantly increased protein carbonyl levels up to almost 5-fold respect to the control, an effect that was reversed by orally administering sLF to mice. Furthermore, clindamycin increased the expression of interleukin-6 and TNF-α by 1- and 2-fold change, respectively. This effect was reversed by treatment with nLF and sLF, decreasing the expression to basal levels. In conclusion, this study indicates that lactoferrin can prevent some of the effects of clindamycin on intestinal cells and their associated immune system.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142387572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interconnected roles of fungal nuclear- and intron-encoded maturases: at the crossroads of mitochondrial intron splicing. 回顾：真菌核和内含子编码成熟酶的相互关联作用：线粒体内含子剪接的十字路口。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-10-01 Epub Date: 2024-06-04 DOI: 10.1139/bcb-2024-0046

Jigeesha Mukhopadhyay, Georg Hausner

{"title":"Interconnected roles of fungal nuclear- and intron-encoded maturases: at the crossroads of mitochondrial intron splicing.","authors":"Jigeesha Mukhopadhyay, Georg Hausner","doi":"10.1139/bcb-2024-0046","DOIUrl":"10.1139/bcb-2024-0046","url":null,"abstract":"Group I and II introns are large catalytic RNAs (ribozymes) that are frequently encountered in fungal mitochondrial genomes. The discovery of respiratory mutants linked to intron splicing defects demonstrated that for the efficient removal of organellar introns there appears to be a requirement of protein splicing factors. These splicing factors can be intron-encoded proteins with maturase activities that usually promote the splicing of the introns that encode them (cis-acting) and/or nuclear-encoded factors that can promote the splicing of a range of different introns (trans-acting). Compared to plants organellar introns, fungal mitochondrial intron splicing is still poorly explored, especially in terms of the synergy of nuclear factors with intron-encoded maturases that has direct impact on splicing through their association with intron RNA. In addition, nuclear-encoded accessory factors might drive the splicing impetus through translational activation, mitoribosome assembly, and phosphorylation-mediated RNA turnover. This review explores protein-assisted splicing of introns by nuclear and mitochondrial-encoded maturases as a means of mitonuclear interplay that could respond to environmental and developmental factors promoting phenotypic adaptation and potentially speciation. It also highlights key evolutionary events that have led to changes in structure and ATP-dependence to accommodate the dual functionality of nuclear and organellar splicing factors.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-520e and its promoter region DNA methylation as potential biomarkers in atherosclerosis. 作为动脉粥样硬化潜在生物标志物的 miR-520e 及其启动子区域 DNA 甲基化。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-10-01 Epub Date: 2024-06-25 DOI: 10.1139/bcb-2023-0326

Mimi Mu, Gao Liu, Xiaoyu Ding, Lijun Xue, Dandan Li, Yunhua Zhu, Nan Zhang, Jia Wu, Junjun Wang

{"title":"miR-520e and its promoter region DNA methylation as potential biomarkers in atherosclerosis.","authors":"Mimi Mu, Gao Liu, Xiaoyu Ding, Lijun Xue, Dandan Li, Yunhua Zhu, Nan Zhang, Jia Wu, Junjun Wang","doi":"10.1139/bcb-2023-0326","DOIUrl":"10.1139/bcb-2023-0326","url":null,"abstract":"In atherosclerosis, DNA methylation plays a key regulatory role in the expression of related genes. However, the molecular mechanisms of these processes in human umbilical vein endothelial cells (HUVECs) are unclear. Here, using high-throughput sequencing from the Infinium HumanMethylation450 assay, we manifested that the cg19564375 methylation of miR-520e promoter region in the peripheral blood of acute coronary syndrome (ACS) patients was higher than that of healthy controls. As shown by RQ-MSP, the upstream DNA methylation level of the miR-520e promoter region was considerably increased in ACS patients. miR-520e was markedly downregulated in ACS patients compared with healthy controls. In the oxidized low-density lipoprotein (ox-LDL)-induced HUVECs injury model, DNA methylation of the upstream region of miR-520e was significantly increased. With increasing concentrations of the methylase inhibitor 5-Aza, miR-520e expression was upregulated. The silence of methyltransferase DNMT1, rather than DNMT3a or DNMT3b, abolished the influence of miR-520e expression by ox-LDL treatment in HUVECs. A dual luciferase reporter assay revealed that miR-520e regulated the TGFBR2 3'-untranslated region region. After silencing TGFBR2, the promoting effect of miR-520e inhibitor on cell proliferation and migration may be attenuated. In conclusion, the expression of miR-520e is modified by its promoter region DNA methylation, and miR-520e and its promoter region DNA methylation may be potential biomarkers in atherosclerosis.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141449559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling stem-like traits and chemoresistance mechanisms in ovarian cancer cells through the TGFβ1-PITX2A/B signaling axis. 通过 TGFβ1-PITX2A/B 信号轴揭示卵巢癌细胞的干样特征和化疗耐受机制

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-10-01 Epub Date: 2024-07-08 DOI: 10.1139/bcb-2024-0010

Sampurna Ghosh, Sk Eashayan Tanbir, Tulika Mitra, Sib Sankar Roy

{"title":"Unveiling stem-like traits and chemoresistance mechanisms in ovarian cancer cells through the TGFβ1-PITX2A/B signaling axis.","authors":"Sampurna Ghosh, Sk Eashayan Tanbir, Tulika Mitra, Sib Sankar Roy","doi":"10.1139/bcb-2024-0010","DOIUrl":"10.1139/bcb-2024-0010","url":null,"abstract":"Ovarian cancer (OC) is the deadliest gynecological malignancy, having a high mortality rate due to its asymptomatic nature, chemoresistance, and recurrence. However, the proper mechanistic knowledge behind these phenomena is still inadequate. Cancer recurrence is commonly observed due to cancer stem cells which also show chemoresistance. We aimed to decipher the molecular mechanism behind chemoresistance and stemness in OC. Earlier studies suggested that PITX2, a homeobox transcription factor and, its different isoforms are associated with OC progression upon regulating different signaling pathways. Moreover, they regulate the expression of drug efflux transporters in kidney and colon cancer, rendering chemoresistance properties in the tumor cell. Considering these backgrounds, we decided to look for the role of PITX2 isoforms in promoting stemness and chemoresistance in OC cells. In this study, PITX2A/B has been shown to promote stemness and to enhance the transcription of ABCB1. PITX2 has been discovered to augment ABCB1 gene expression by directly binding to its promoter. To further investigate the regulatory mechanism of PITX2 gene expression, we found that TGFβ signaling could augment the PITX2A/B expression through both SMAD and non-SMAD signaling pathways. Collectively, we conclude that TGFβ1-activated PITX2A/B induces stem-like features and chemoresistance properties in the OC cells.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141557899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuroprotective effects of whey and buttermilk-based formulas on a DSS-induced colitis murine model. 乳清和酪乳配方奶粉对 DSS 诱导的小鼠结肠炎模型的神经保护作用。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-10-01 DOI: 10.1139/bcb-2024-0153

Berta Buey, Eva Latorre, Marta Castro, Marta Sofía Valero, Miguel Ángel Plaza, María Pilar Arruebo, Inés Abad, Ana Rodríguez-Largo, Lourdes Sánchez, José Emilio Mesonero

{"title":"Neuroprotective effects of whey and buttermilk-based formulas on a DSS-induced colitis murine model.","authors":"Berta Buey, Eva Latorre, Marta Castro, Marta Sofía Valero, Miguel Ángel Plaza, María Pilar Arruebo, Inés Abad, Ana Rodríguez-Largo, Lourdes Sánchez, José Emilio Mesonero","doi":"10.1139/bcb-2024-0153","DOIUrl":"10.1139/bcb-2024-0153","url":null,"abstract":"Inflammatory bowel disease is a gut-brain axis disorder that comprises chronic inflammatory conditions affecting the gastrointestinal tract, where alterations in the mood of patients are common. Gut-brain axis is a bidirectional communication that link gut and brain. The close association between inflammatory bowel disease and neuroinflammation has far-reaching implications, as is increasingly recognized as a contributing factor to neuropsychiatric and neurodegenerative diseases. The increasing prevalence and high economic cost, together with the loss of life quality of people suffering from these diseases, point to the need to find alternatives to alleviate them. Exploring new therapeutic avenues prompts us to consider the potential benefits of milk fractions, taking advantage of the use of dairy by-products, such as whey and buttermilk. This study examines the impact of cow's whey- and buttermilk-based formulas supplemented with bovine lactoferrin and milk fat globule membrane on the expression of cytokines, as well as on the components of immune and serotonergic system of the brain in a murine model of dextran sodium sulfate-induced colitis. Our results show the potential of these dairy by-products, especially whey, as functional foods in ameliorating neuroinflammation and safeguarding the central nervous system function amid the neurological complications induced or concomitant with intestinal inflammatory processes.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142364213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skeletal muscle mitochondrial morphology negatively affected in mice lacking Xin. 缺乏欣的小鼠骨骼肌线粒体形态受到负面影响

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-10-01 Epub Date: 2024-06-06 DOI: 10.1139/bcb-2024-0034

Grace Martin, Dhuha Al-Sajee, Molly Gingrich, Rimsha Chattha, Michael Akcan, Cynthia M F Monaco, Megan C Hughes, Christopher G R Perry, Irena A Rebalka, Mark A Tarnopolsky, Thomas J Hawke

{"title":"Skeletal muscle mitochondrial morphology negatively affected in mice lacking Xin.","authors":"Grace Martin, Dhuha Al-Sajee, Molly Gingrich, Rimsha Chattha, Michael Akcan, Cynthia M F Monaco, Megan C Hughes, Christopher G R Perry, Irena A Rebalka, Mark A Tarnopolsky, Thomas J Hawke","doi":"10.1139/bcb-2024-0034","DOIUrl":"10.1139/bcb-2024-0034","url":null,"abstract":"Altered mitochondrial structure and function are implicated in the functional decline of skeletal muscle. Numerous cytoskeletal proteins are known to affect mitochondrial homeostasis, but this complex network is still being unraveled. Here, we investigated mitochondrial alterations in mice lacking the cytoskeletal adapter protein, XIN (XIN-/-). XIN-/- and wild-type littermate male and female mice were fed a chow or high-fat diet (HFD; 60% kcal fat) for 8 weeks before analyses of their skeletal muscles were conducted. Immuno-electron microscopy (EM) and immunofluorescence staining revealed XIN in the mitochondria and peri-mitochondrial areas, as well as the myoplasm. Intermyofibrillar mitochondria in chow-fed XIN-/- mice were notably different from wild-type (large, and/or swollen in appearance). Succinate dehydrogenase and Cytochrome Oxidase IV staining indicated greater evidence of mitochondrial enzyme activity in XIN-/- mice. No difference in body mass gains or glucose handling was observed between cohorts with HFD. However, EM revealed significantly greater mitochondrial density with evident structural abnormalities (swelling, reduced cristae density) in XIN-/- mice. Absolute Complex I and II-supported respiration was not different between groups, but relative to mitochondrial density, was significantly lower in XIN-/-. These results provide the first evidence for a role of XIN in maintaining mitochondrial morphology and function.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141282912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0