Biochemistry and Cell Biology最新文献

Biomolecular condensates orchestrate small RNA pathways and transgenerational inheritance. 生物分子凝聚体协调小RNA途径和跨代遗传。

IF 2.2 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-10-28 DOI: 10.1139/bcb-2026-0206

Liangtao Chen, Ke Wang

引用次数: 0

Abrogation of EMP1 Mitigates MASH-Associated Hepatic Fibrosis through Suppression of the JAK/STAT Signaling Axis. 废除EMP1通过抑制JAK/STAT信号轴减轻mash相关的肝纤维化。

IF 2.2 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-10-23 DOI: 10.1139/bcb-2026-0099

Wendi Wang, Wendi Wang, Shangbo Jin, Di Wang, Xinyue Xie, Chiyi Chen, Li Zhang, Qingjun Guo, Xiaoran Qin, Bowen Bi, Xin Li, Yan Xie, Wentao Jiang

{"title":"Abrogation of EMP1 Mitigates MASH-Associated Hepatic Fibrosis through Suppression of the JAK/STAT Signaling Axis.","authors":"Wendi Wang, Wendi Wang, Shangbo Jin, Di Wang, Xinyue Xie, Chiyi Chen, Li Zhang, Qingjun Guo, Xiaoran Qin, Bowen Bi, Xin Li, Yan Xie, Wentao Jiang","doi":"10.1139/bcb-2026-0099","DOIUrl":"https://doi.org/10.1139/bcb-2026-0099","url":null,"abstract":"Metabolic dysfunction-associated steatohepatitis (MASH) is a progressive liver disease driven by fibrogenesis, posing high risks for cirrhosis and cancer. Epithelial membrane protein 1 (EMP1) is a biomarker in fibrosis, but its role in MASH is unclear.EMP1 expression was assessed in MASH mouse models (MCD/HFD-induced) and human MASH specimens. The functional effects of EMP1 silencing and overexpression were evaluated in vivo, focusing on steatosis, inflammation, and fibrosis. Mechanistically, the interaction between EMP1 and the JAK/STAT pathway was examined. EMP1 expression was significantly upregulated in both murine MASH models and human MASH liver tissues compared to healthy controls. Functionally, in vivo silencing of EMP1 markedly attenuated hepatic lipid accumulation, inflammatory infiltration, and fibrogenesis in mice fed MCD or HFD. Conversely, EMP1 overexpression exacerbated MASH progression, with a pronounced effect on fibrogenesis. Mechanistic studies demonstrated that EMP1 promotes the phosphorylation of STAT1 and STAT3 via its specific interaction with JAK2, whereas silencing EMP1 disrupts JAK2-mediated activation of downstream signal transducers. This inhibition of the JAK/STAT axis resulted in the suppression of hepatic stellate cell (HSC) activation, a key event in fibrogenesis. Our findings establish EMP1 as a critical pathogenic mediator in MASH-related fibrosis. By directly regulating the JAK2/STAT1/3 signaling axis in HSCs, EMP1 promotes fibrogenesis, highlighting its potential as a novel therapeutic target for the treatment of MASH.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149242779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct effects of disease-associated mutations in the proximal C-linker on negatively cooperative binding of cyclic nucleotides to the human HCN4 channel. 近端c连接体疾病相关突变对环核苷酸与人类HCN4通道负合作结合的独特影响

IF 2.2 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2026-0147

Leo C T Ng, Joanna Xia, Filip Van Petegem, Eric A Accili

{"title":"Distinct effects of disease-associated mutations in the proximal C-linker on negatively cooperative binding of cyclic nucleotides to the human HCN4 channel.","authors":"Leo C T Ng, Joanna Xia, Filip Van Petegem, Eric A Accili","doi":"10.1139/bcb-2026-0147","DOIUrl":"10.1139/bcb-2026-0147","url":null,"abstract":"The direct binding of cAMP to the hyperpolarization-activated cyclic nucleotide-gated channel form 4 (HCN4) protein contributes to autonomic modulation of heart rate. Here, we determine affinity for binding of cyclic nucleotides directly to the cytosolic C-terminus of the HCN4 channel of three disease-linked mutations that are found in the C-linker of the C-terminus, which attaches the more distal binding domain to the pore. Two of these mutations, one in the A'-helix (K530N) and another in the B'-helix (D553N), reduced binding affinity but did not alter the negatively cooperative pattern of binding found in the wild type channel. Finally, a third mutation in the A'-helix (R524Q) did not alter either the pattern or affinity of binding to the cyclic nucleotides despite a dramatic, and previously reported, increase in cAMP potency in the full channel. Our data may be explained by location of each mutation and its interactions with domains external to this region. The data support negatively cooperative binding of cAMP and cGMP to HCN4 and help to identify distinct structural contributions to ligand binding and gating, both of which determine potency.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-16"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148161514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diphenoquinone, a small-HDL neogenesis accelerator, concurrently docks with cholesterol or POPC to human ATP-binding cassette transporter ABCA1. 二苯醌，一种小hdl新生促进剂，与胆固醇或POPC同时对接人类atp结合盒转运蛋白ABCA1。

IF 2.2 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2026-0043

Maki Tsujita, Leticia Alves da Silva, Kosuke Nakasuka, Junki Yamamoto, Robert C Ford, Thomas Stockner

{"title":"Diphenoquinone, a small-HDL neogenesis accelerator, concurrently docks with cholesterol or POPC to human ATP-binding cassette transporter ABCA1.","authors":"Maki Tsujita, Leticia Alves da Silva, Kosuke Nakasuka, Junki Yamamoto, Robert C Ford, Thomas Stockner","doi":"10.1139/bcb-2026-0043","DOIUrl":"10.1139/bcb-2026-0043","url":null,"abstract":"High-density lipoprotein (HDL) functionality has emerged as a key determinant of residual cardiovascular risk despite advances in low-density lipoprotein-cholesterol lowering therapies. Small HDL particles, generated through ATP-binding cassette transporter A1 (ABCA1)-mediated lipid efflux, are particularly effective in promoting cholesterol removal from macrophage foam cells and are associated with reduced atherosclerotic risk. Probucol, a lipid-lowering drug, inhibits ABCA1-dependent HDL biogenesis, whereas its major metabolite, 3,3',5,5'-tetra-tert-butyldiphenoquinone (DQ), preserves the generation of small HDL particles. However, the molecular basis for these distinct effects has remained unclear. To investigate the structural mechanisms underlying ABCA1-mediated lipid export, we performed molecular docking analyses using the human ABCA1 structure (Protein Data Bank, PDB: 5XJY) and the Genetic Optimization for Ligand Docking in silico docking platform. Cholesterol and 1-palmitoyl-2-oleoyl-phosphatidylcholine docking poses were successfully identified in DQ-bound ABCA1. In contrast, no ligand-binding poses were detected in probucol-bound ABCA1. These findings suggest that DQ preserves the lipid-recognition and transport properties of ABCA1, whereas probucol disrupts ligand accommodation within the transporter. This study provides the first atomic-level insight into the interactions among ABCA1, cholesterol, probucol, and DQ, offering a structural explanation for their distinct effects on HDL biogenesis and identifying a potential framework for developing novel HDL-targeted therapeutics.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-12"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148683250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discussion of "Efficacy of lactoferrin supplementation in pediatric infections: a systematic review and meta-analysis". 关于“补充乳铁蛋白对儿童感染的疗效：一项系统回顾和荟萃分析”的讨论。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2025-0234

Graziella D'Arrigo, Mercedes Gori

引用次数: 0

USP54 regulates the malignancy and autophagy in nasopharyngeal cancer cells by modulating ULK1 ubiquitination levels. USP54通过调节ULK1泛素化水平调控鼻咽癌细胞的恶性和自噬。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2025-0235

Fanrui Fu, Yong Wu, Peilin Wang, Wubin Yuan, Yi Jiang

{"title":"USP54 regulates the malignancy and autophagy in nasopharyngeal cancer cells by modulating ULK1 ubiquitination levels.","authors":"Fanrui Fu, Yong Wu, Peilin Wang, Wubin Yuan, Yi Jiang","doi":"10.1139/bcb-2025-0235","DOIUrl":"10.1139/bcb-2025-0235","url":null,"abstract":"Nasopharyngeal carcinoma (NPC) is an aggressive malignancy with poor outcomes at advanced stages. Although ubiquitination is critical in regulating oncogenic pathways, the role of many ubiquitin-specific proteases (USPs) in NPC remains unexplored. In this study, we conducted a systematic screen of USPs to identify regulators of ULK1, a key initiator of autophagy and oncogene in NPC. We identified USP54 as a potent stabilizer of ULK1. Bioinformatic analyses of NPC transcriptomic datasets revealed that both ULK1 and USP54 are significantly overexpressed in NPC tissues. Functional assays demonstrated that USP54 overexpression enhanced NPC cell viability, colony formation, migration, and invasion by promoting autophagy, independently of PI3K/AKT pathway activity. Importantly, pharmacological inhibition of autophagy or genetic silencing of ULK1 abolished the oncogenic effects of USP54, indicating that ULK1-dependent autophagy mediates USP54-driven NPC progression. Our findings revealed that USP54 may promote autophagy and malignancy in NPC via regulating ULK1 stability, highlighting USP54 as a potential therapeutic target for NPC treatment.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-12"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145740750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Terpenes as modulators of nociceptive signaling: behavioral and molecular insights from Caenorhabditis elegans. 萜烯作为伤害性信号的调节剂：秀丽隐杆线虫的行为和分子观察。

IF 2.2 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2025-0380

Kaoutar Benkhraba, Fatma Boujenoui, Jesus D Castaño, Jabin Sultana, Francis Beaudry

{"title":"Terpenes as modulators of nociceptive signaling: behavioral and molecular insights from Caenorhabditis elegans.","authors":"Kaoutar Benkhraba, Fatma Boujenoui, Jesus D Castaño, Jabin Sultana, Francis Beaudry","doi":"10.1139/bcb-2025-0380","DOIUrl":"10.1139/bcb-2025-0380","url":null,"abstract":"Terpenes such as Limonene (LIMO) and β-Caryophyllene (BC) have demonstrated pain-modulating properties, potentially through interactions with the Transient receptor potential vanilloid 1 (TRPV1) receptor. This study examines the antinociceptive effects of four terpenes derived from Cannabis sativa: Limonene, β-Caryophyllene, α-Humulene (HUM), and α-Myrcene (MYR) using Caenorhabditis elegans (C. elegans). The primary objective was to characterize terpene-induced modulation of nocifensive responses to noxious heat, and to elucidate their influence on molecular pathways via specific receptor targets. Thermotaxis assays quantified the antinociceptive activity of increasing terpene concentrations in wild-type nematodes. To assess receptor-specific mechanisms, assays were performed in mutant strains lacking functional OCR-2 and OSM-9 (TRPV-like nociceptors) and NPR-19 and NPR-32 (encoding cannabinoid-like receptors). Proteomic profiling coupled with bioinformatics analysis identified terpene-induced alterations in signaling pathways and biological processes. All four terpenes exhibited significant antinociceptive activity in wild-type C. elegans, with impaired effects observed in vanilloid receptor mutants, implicating TRPV-like channels in their mechanism of action. Proteomic and pathway analyses revealed terpene-specific molecular signatures, highlighting differential modulation of neuronal and stress-responsive signaling cascades. By elucidating the molecular mechanisms underlying terpene-induced nociceptive modulation, this work strengthens the growing body of evidence supporting the therapeutic promise of terpenes in pain management outside the effect referred to as the \"entourage effect\".","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-16"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meeting report: 3rd Codon Usage Conference: Function, Mechanism and Evolution. 会议报告：第三届密码子使用会议：功能、机制与演变。

IF 2.2 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2026-0275

Alexander F Palazzo

引用次数: 0

Chelerythrine alleviates inflammation and angiogenesis in a mouse rosacea model via suppressing the NF-κB/p38 MAPK/STAT3 pathways. 车车草碱通过抑制NF-κB/p38 MAPK/STAT3通路减轻小鼠酒痤疮模型的炎症和血管生成。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2025-0124

Rong Zhou, Zhibo Yang, Junwen Wang, Chang Wang, Meijunzi Luo, Yi Pan, Pan Huang, Yi-Ning Yan, Di Long, Haizhen Wang

{"title":"Chelerythrine alleviates inflammation and angiogenesis in a mouse rosacea model via suppressing the NF-κB/p38 MAPK/STAT3 pathways.","authors":"Rong Zhou, Zhibo Yang, Junwen Wang, Chang Wang, Meijunzi Luo, Yi Pan, Pan Huang, Yi-Ning Yan, Di Long, Haizhen Wang","doi":"10.1139/bcb-2025-0124","DOIUrl":"10.1139/bcb-2025-0124","url":null,"abstract":"Rosacea is a chronic inflammatory skin condition marked by excessive M1 macrophage polarization and angiogenesis, resulting in erythema and tissue inflammation. Despite available treatments, many patients experience recurrent flare-ups. This study explores chelerythrine, a bioactive component of Phellodendri Chinensis Cortex, for its therapeutic potential in rosacea through modulation of NF-κB, p38 MAPK, and STAT3 signaling, inflammation, and vascular regulation. Using an LL-37-induced rosacea-like mouse model, THP-1-derived M1 macrophages and human umbilical vein endothelial cells (HUVECs), chelerythrine's effects on macrophage polarization, cytokine expression, angiogenesis, and pathway activation of NF-κB, p38 MAPK, and STAT3 were evaluated. Chelerythrine significantly reduced epidermal thickness, inflammatory cell infiltration, and pro-inflammatory markers (TNF-α and IL-1β). It inhibited NF-κB, p38 MAPK, and STAT3 activation and decreased M1 polarization markers, shifting towards an anti-inflammatory profile. Furthermore, chelerythrine reduced vascular density and VEGF expression, impairing angiogenesis-related behaviors in HUVECs. These findings suggest that chelerythrine holds promise as a treatment for rosacea by mitigating inflammation and angiogenesis through targeted multiple pathways and macrophage modulation.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-12"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An improved version of the early histone HCl extraction protocol. 早期组蛋白HCl提取方案的改进版本。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2026-01-01 DOI: 10.1139/bcb-2025-0335

Geneveve Analike, Dhanvi Prajapati, James R Davie, Juan Ausió

引用次数: 0