Biochemical Engineering Journal最新文献_第8页

One-step solvothermal preparation of Fe3O4@HA magnetic nanomicrospheres for simultaneous removal of tetracycline antibiotics

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-01-29 DOI: 10.1016/j.bej.2025.109651

Sisi Li , Shuang Li , Xiang Li , Xiaodan Zeng , Jie Chen , Zhigang Liu , Shihua Yu , Junjing Hao

{"title":"One-step solvothermal preparation of Fe3O4@HA magnetic nanomicrospheres for simultaneous removal of tetracycline antibiotics","authors":"Sisi Li , Shuang Li , Xiang Li , Xiaodan Zeng , Jie Chen , Zhigang Liu , Shihua Yu , Junjing Hao","doi":"10.1016/j.bej.2025.109651","DOIUrl":"10.1016/j.bej.2025.109651","url":null,"abstract":"<div><div>Humic acid (HA) is a naturally biomass, that is considered as an ideal adsorbent material due to the abundance of functional groups on its surface. For this purpose, the highly dispersible magnetic humic acid (Fe<sub>3</sub>O<sub>4</sub>@HA) has been prepared by one-step solvothermal method. The developed Fe<sub>3</sub>O<sub>4</sub>@HA not only has a strong separation, but also can simultaneously adsorb tetracycline antibiotics (tetracycline, chlortetracycline hydrochloride and doxycycline hydrochloride). Batch sorption experiments convinced that the maximum tetracycline antibiotics adsorption capacity of Fe<sub>3</sub>O<sub>4</sub>@HA is 45.58, 86.87, and 88.46 mg/g and the largest removal of 94.5 %, 96.3 %, and 97.4 %, fast equilibrium time of 60 min. Meanwhile, the adsorption process is agreement with pseudo-second-order adsorption kinetics and Langmuir adsorption isotherm model. Thermodynamic data indicate that this adsorption is endothermic and spontaneous. Moreover, the designed Fe<sub>3</sub>O<sub>4</sub>@HA with the characteristic of anti-interference capabilities, a variety of water-matrix suitability and maintaining at least 80 % tetracycline antibiotics removal after five adsorption cycles. Finally, the adsorption mechanisms are explored by XPS and FT-IR analysis, which show that mainly involve electrostatic adsorption; hydrogen bonding, π–π, oxygen-containing functional group interactions; and physical adsorption on the surface. In conclusion, Fe<sub>3</sub>O<sub>4</sub>@HA demonstrates considerable promise as an adsorbent for treating antibiotic-contaminated water.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109651"},"PeriodicalIF":3.7,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143335201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance and phosphorus release of an anaerobic co-digestion of excess sludge and food waste containing garlic at varying substrate ratio

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-01-29 DOI: 10.1016/j.bej.2025.109659

Lixin Wei , Yuchen Wang , Yuan He , Guangrong Zhou , Shutong Dong , Yongan Chen , Qing Wang , Yuxiang Lu , Chengyuan Su

{"title":"Performance and phosphorus release of an anaerobic co-digestion of excess sludge and food waste containing garlic at varying substrate ratio","authors":"Lixin Wei , Yuchen Wang , Yuan He , Guangrong Zhou , Shutong Dong , Yongan Chen , Qing Wang , Yuxiang Lu , Chengyuan Su","doi":"10.1016/j.bej.2025.109659","DOIUrl":"10.1016/j.bej.2025.109659","url":null,"abstract":"<div><div>The effects of different ratios of food waste (FW) containing garlic and excess sludge (ES) on the anaerobic co-digestion (AcoD) process were investigated. This was done by establishing a sequencing batch anaerobic digester and analyzing phosphorus release patterns, microbial community structure and gene function in the AcoD system with 1:1, 1:2, 1:4, and 1:8 ratio of FW to ES. The highest phosphine (PH<sub>3</sub>) gas output of 1619.99 mg/m<sup>3</sup> was observed when FW:ES was 1:1. The relative abundance of <em>Methanothrix</em> increased from 7.99 % to 9.36 % with variation the ratio of FW:ES from 1:4–1:8. The contents of <em>phnD</em> and <em>phnE</em> genes in the ratio of 1:4 FW:ES group were the highest among all experimental groups, with 0.0010 % and 0.0018 %, respectively. In addition, the abundance of functional genes responsible for encoding acetate kinase in the 1:4 FW:ES group increased by 152.96 % relative to the control group. Tests determined the optimal FW to ES ratios to achieve 1:4 and 1:8, and provided suitable operating conditions and references for the subsequent AcoD research.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109659"},"PeriodicalIF":3.7,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable production of plant biostimulants from cephalosporin fermentation residues: Ultrasonic dissolution and enzymatic hydrolysis

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-01-28 DOI: 10.1016/j.bej.2025.109658

Dongze Niu , Peiwen Ding , Wenying An , Chunyu Li , Dongmin Yin , Taoli Huhe , Jianjun Ren

{"title":"Sustainable production of plant biostimulants from cephalosporin fermentation residues: Ultrasonic dissolution and enzymatic hydrolysis","authors":"Dongze Niu , Peiwen Ding , Wenying An , Chunyu Li , Dongmin Yin , Taoli Huhe , Jianjun Ren","doi":"10.1016/j.bej.2025.109658","DOIUrl":"10.1016/j.bej.2025.109658","url":null,"abstract":"<div><div>Cephalosporin fermentation residues (CFR) is one of the most challenging industrial organic wastes due to their high nitrogen content. To transform the waste into high-value products, this study explored the effects of various factors on the solubility of total nitrogen (TN) and amino acids (AAs) and the enzymatic hydrolysis characteristics of proteins in CFR and evaluated the bioactivity of the hydrolysates through soybean germination experiments. Results showed that the solubility of AAs and TN reached their peaks at pH = 12 and 50℃, and ultrasonic treatment for 4 h further improved their dissolution. After 24 hours of hydrolysis, the AA yield in the group combining ultrasonication and enzymatic hydrolysis reached 217.5 g/kg dry matter, an 8 % increase compared to the control. The contents of some AAs, such as glutamic acid, phenylalanine, histidine, and arginine, increased by 43–85 % in the hydrolysate. Fluorescence spectroscopy confirmed the release of aromatic amino acids, such as tryptophan and tyrosine, which are known to improve plant stress tolerance. Furthermore, the diluted hydrolysates significantly promoted soybean seed germination under cold and saline conditions (<em>P</em> < 0.05), with the highest germination rates observed at a 1000x dilution. These findings highlight the effectiveness of enzymatic hydrolysis in transforming CFR into plant biostimulants, offering a sustainable solution for agricultural and environmental applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109658"},"PeriodicalIF":3.7,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving the fermentability of dilute acid hydrolysate and recovering low-concentration p-toluenesulfonic acid based on a fixed-bed column with hyper-cross-linked resin

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-01-27 DOI: 10.1016/j.bej.2025.109657

Junjie Hao , Hailong Li , Xuefang Chen , Zhijie Shen , Chaoqin Zhang , Zhen Xiong , Can Wang , Haijun Guo , Hairong Zhang , Lian Xiong , Sansan Yu , Xinde Chen

{"title":"Improving the fermentability of dilute acid hydrolysate and recovering low-concentration p-toluenesulfonic acid based on a fixed-bed column with hyper-cross-linked resin","authors":"Junjie Hao , Hailong Li , Xuefang Chen , Zhijie Shen , Chaoqin Zhang , Zhen Xiong , Can Wang , Haijun Guo , Hairong Zhang , Lian Xiong , Sansan Yu , Xinde Chen","doi":"10.1016/j.bej.2025.109657","DOIUrl":"10.1016/j.bej.2025.109657","url":null,"abstract":"<div><div>As the closest pretreatment to industrialization, dilute acid hydrolysis is effective in producing fermentable sugars from lignocellulose. However, the reuse of acid catalysts is a difficult problem that restricts the economy of the biorefining process. In addition, inhibitors generated during dilute acid pretreatment seriously affect the bioconversion of fermentable sugars. In this study, the dynamic adsorption and desorption of dilute acid hydrolysate on a fixed-bed column with hyper-cross-linked resin were investigated to improve the fermentability of hydrolysate by removing inhibitors and recover the low-concentration <em>p</em>-toluenesulfonic acid (<em>p</em>-TsOH) for cyclic pretreatment. The results showed that <em>p</em>-TsOH can be recovered by dynamic adsorption and desorption for cyclic pretreatment, which released similar concentration of xylose compared with the first pretreatment. Moreover, the total sugar loss rate was less than 2 % after adsorption. The fermentability of hydrolysate after adsorption by the fixed-bed column was greatly improved. The fermentation performance of <em>Trichosporon cutaneum</em> (<em>T. cutaneum</em>) was enhanced with a yeast biomass of 19.26 g/L, lipid content of 25.62 %, and lipid yield of 4.93 g/L. Compared with the fermentation results of hydrolysate without detoxification, the lipid content and lipid yield increased by 67.12 % and 94.10 %, respectively. In summary, this study provides a feasible technology for improving the fermentability of dilute acid hydrolysate and recovering acid catalysts.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109657"},"PeriodicalIF":3.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An optimized triple-plasmid system with enhanced viral and helper gene expression for improved recombinant adeno-associated virus production

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-01-27 DOI: 10.1016/j.bej.2025.109652

Qian Ye , Ruirui Li , Zhiying Xiao , Daoyuan Na , Naixiang Cui , Liang Zhao , Wen-Song Tan

{"title":"An optimized triple-plasmid system with enhanced viral and helper gene expression for improved recombinant adeno-associated virus production","authors":"Qian Ye , Ruirui Li , Zhiying Xiao , Daoyuan Na , Naixiang Cui , Liang Zhao , Wen-Song Tan","doi":"10.1016/j.bej.2025.109652","DOIUrl":"10.1016/j.bej.2025.109652","url":null,"abstract":"<div><div>Recombinant adeno-associated virus (rAAV) is a preferred gene therapy vector due to its safety and efficacy. The predominant production method relies on a transient expression system utilizing mammalian cells, though low production efficiency and high dosage requirements increase costs and limit large-scale applications. The triple-plasmid system forms the basis of rAAV transient expression, where the genetic elements and their configurations significantly impact production efficiency. Here, we developed two novel triple-plasmid transient expression systems, AAV-RepE4 and AAV-RepDBP, leveraging insights into essential viral gene elements and helper components. The rAAV titer of HEK293F cells transfected with the AAV-RepE4 system increased by 2.6-fold compared to the traditional triple-plasmid system, and its single-cell packaging capacity improved by 2.2-fold. Notably, the AAV-RepE4 system exhibited higher expression levels of <em>rep52/rep40</em> and a relatively moderate upregulation of <em>rep78/rep68</em>. This optimization facilitated viral genome synthesis and packaging while supporting favorable host cell conditions. These findings offer valuable insights into the characteristics of the viral vector production process and offer theoretical guidance and new strategies for optimizing gene therapy vector manufacturing.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109652"},"PeriodicalIF":3.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143379194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of serum on chondrogenic differentiation and proliferation of 2D cultured rat bone marrow mesenchymal stem cells

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-01-27 DOI: 10.1016/j.bej.2025.109653

Xueqiang Guo , Lingling Xi , Jixiang Li , Mengyuan Yu , Mengnan Wen , Guangdong Zhou , Wenjie Ren

{"title":"Effect of serum on chondrogenic differentiation and proliferation of 2D cultured rat bone marrow mesenchymal stem cells","authors":"Xueqiang Guo , Lingling Xi , Jixiang Li , Mengyuan Yu , Mengnan Wen , Guangdong Zhou , Wenjie Ren","doi":"10.1016/j.bej.2025.109653","DOIUrl":"10.1016/j.bej.2025.109653","url":null,"abstract":"<div><div>Bone marrow-derived mesenchymal stem cells (BMSCs) are considered the \"golden standard\" of seeding cell for cartilage or bone tissue engineering, but there are inconsistent reports regarding the use and concentration of serum on chondrogenic differentiation of BMSCs. We aim to investigate the effect of serum on chondrogenic differentiation and proliferation of BMSCs in 2D culture. Microscopic observation and counting of the effects of different serum concentrations (0 %, 0.5 %, 1 %, 2 %, and 5 %) on the cell aggregation of BMSCs induced for different durations (7 d, 14 d, and 21 d) revealed that an appropriate concentration of serum (1–5 %) facilitated the formation of cell aggregates during BMSCs chondrogenic differentiation in 2D culture; CCK-8 revealed that serum on cell proliferation was not significantly different, but live/dead staining showed that serum offered cells a favorable living environment resulting a good activity for BMSCs chondrogenic differentiation; Histological examination found no significant difference in different serum concentrations on BMSCs chondrogenic differentiation, suggesting that serum may not be a decisive factor in chondrogenic differentiation. In conclusion, serum can significantly promote cell microsphere formation and growth, which is beneficial to the automatic transformation from 2D to 3D due to offering cells a favorable living environment. It has important guiding significance for the large-scale formation of cell microspheres, which is recognized to contribute to the repair of osteochondral injury.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109653"},"PeriodicalIF":3.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ferrous step-change method for kLa measurement in an airlift bioreactor containing iron sulfate broth for energy storage

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-01-26 DOI: 10.1016/j.bej.2025.109656

Jason Glas, Dimitre Karamanev

{"title":"Ferrous step-change method for kLa measurement in an airlift bioreactor containing iron sulfate broth for energy storage","authors":"Jason Glas, Dimitre Karamanev","doi":"10.1016/j.bej.2025.109656","DOIUrl":"10.1016/j.bej.2025.109656","url":null,"abstract":"<div><div>The transition to renewable energy will require significant grid level energy storage capacity to maintain electrical supply and demand balance. A biological hydrogen-based energy storage technology using ferric sulfate as an oxidant in a proton exchange membrane fuel cell, termed the BioGenerator, is studied in this work. Operation of the fuel cell generates ferrous sulfate which must be oxidized through use of iron oxidizing bacteria and oxygen in an airlift reactor. The volumetric oxygen mass transfer coefficient (k<sub>L</sub>a) in the airlift bioreactor is investigated for use in design optimizations to minimize energy expenditure in aeration within the system. A novel high-precision technique of determining k<sub>L</sub>a profiles in bioreactors using aerobic chemolithotrophs in ferric sulfate broths is explored to meet this goal. The new method makes use of measurable ferrous iron bio-oxidation with a redox probe along with bioreaction stoichiometry to solve for oxygen uptake rate. The procedure, deemed the static ferrous step-change method, is compared against the dynamic biological in-situ method and proves to be superior in terms of scalability, time savings, ease of use, and data quality. Aeration mixing effect on dissolved oxygen homogeneity within the bulk liquid in the downcomer packed bed of semi-pilot and pilot scale airlift bioreactors used in the study are investigated. The developed static ferrous-step change method may be applicable for practical use in any operation where aerobic iron-oxidizing acidophilic microbes are present in iron sulfate based liquor or leachate, potentially useful in processes such as copper or uranium extraction.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109656"},"PeriodicalIF":3.7,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced removal of TN in shale gas wastewater by a novel porous polymer monolith immobilized microorganisms

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-01-25 DOI: 10.1016/j.bej.2025.109650

Shuling Shi , Peng Tang , Xiang Zhang , Guijing Chen , Chunyan Yang , Di Zhang , Fengming Li , Mengting Zhu , Ping Yang , Baicang Liu

{"title":"Enhanced removal of TN in shale gas wastewater by a novel porous polymer monolith immobilized microorganisms","authors":"Shuling Shi , Peng Tang , Xiang Zhang , Guijing Chen , Chunyan Yang , Di Zhang , Fengming Li , Mengting Zhu , Ping Yang , Baicang Liu","doi":"10.1016/j.bej.2025.109650","DOIUrl":"10.1016/j.bej.2025.109650","url":null,"abstract":"<div><div>Shale gas flowback and produced water (SGFPW) pose significant challenges to biotechnology due to their high salinity. In this study, we explored a novel method utilizing an amphiphilic copolymer of methyl methacrylate and acrylamide (p(MMA-AAM)) and powdered activated carbon (PAC) to modify poly(ether sulfone) (PES). We prepared a series of monoliths for microorganism immobilization using both thermal-induced phase separation and non-solvent-induced phase separation. Characterization of the monoliths via fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption analysis revealed improved hydrophilicity in the p(MMA-AAM)-modified monoliths and increased specific surface area (SSA) in monoliths with PAC addition. Particularly, the highest SSA was observed for the 0.5 g PAC (0.5-PAC/PES) monolith at 235.19 m<sup>2</sup>/g. Wastewater treatment experiments demonstrated that the 0.5-PAC/PES system exhibited superior pollutant removal performance, and it achieved nearly 100 % removal of NO<sub>3</sub><sup>-</sup> and total nitrogen (TN) in simulated SGFPW. Besides, the removal rates of TN and total organic carbon (TOC) in actual SGFPW by the 0.5-PAC/PES system were 1.5 times and 1.3 times higher, respectively, than those achieved with suspended microorganisms. After 96 h biological treatment, the extracellular polymeric substances (EPS) content of 0.5-PAC/PES was highest, showing its positive role in enhancing microbial activity. This work introduces a new approach to immobilize microorganisms, enhancing biological activity for SGFPW treatment under high salinity conditions.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109650"},"PeriodicalIF":3.7,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143167655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of impeller design on oxygen transfer in a stirred water tank with square cross-section

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-01-24 DOI: 10.1016/j.bej.2025.109649

I. Pincovschi , A. Dragomirescu , C. Modrogan

{"title":"Influence of impeller design on oxygen transfer in a stirred water tank with square cross-section","authors":"I. Pincovschi , A. Dragomirescu , C. Modrogan","doi":"10.1016/j.bej.2025.109649","DOIUrl":"10.1016/j.bej.2025.109649","url":null,"abstract":"<div><div>This paper presents an experimental study on the mass transfer performance in a gas-liquid stirred vessel. Three impellers – circular with flat blades, rectangular with slightly curved blades, and trapezoidal with flat perforated blades – were tested for two aspect ratios of the water column inside a tank. The study aimed at identifying the type of impeller and operating conditions that maximize the aeration efficiency of vortex aerators and at verifying whether the usage of impellers with perforated blades could decrease the energy consumption while maintaining a good aeration efficiency. The results revealed the existence of two critical impeller speeds. At the first critical speed, the operating regime changes from subcritical or non-aerated to supercritical or aerated. The vortex specific interfacial area increases almost linearly up to the second critical speed and then tends to stabilize. The impeller with perforated blades did not confirm the expectations, its efficiency being very poor. The rectangular impeller was the most efficient in the subcritical regime. The circular impeller performed better in the supercritical regime. These two impellers were found to operate roughly in the same range as other conventional impellers for an aspect ratio of 1. This finding, coupled with the geometrical simplicity, recommends the circular and rectangular impellers for usage in tank aerators and, possibly, in bioreactors.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109649"},"PeriodicalIF":3.7,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of adding cold-adapted microbial agent on the decomposition and microbial community diversity of human faecal compost

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-01-21 DOI: 10.1016/j.bej.2025.109642

Xiyan Du, Haihong Li, Hanqing Zou, Fengqiu An

{"title":"Effect of adding cold-adapted microbial agent on the decomposition and microbial community diversity of human faecal compost","authors":"Xiyan Du, Haihong Li, Hanqing Zou, Fengqiu An","doi":"10.1016/j.bej.2025.109642","DOIUrl":"10.1016/j.bej.2025.109642","url":null,"abstract":"<div><div>This study tackled the challenges posed by arid and cold climates on human faeces treatment technology. Three strains of faecal degrading bacteria were previously isolated and purified from cold environments using a gradient dilution method. Rice husk powder was used as a carrier to prepare cold-adapted microbial agent (CAMA). To study the effect of CAMA, a 35-day composting experiment was conducted at 5°C–10°C using wood chips as a conditioning agent. The experiment was divided into two groups: the experimental group (S), which contained CAMA, and the control group (CK), which did not contain CAMA. Inoculation with CAMA caused the compost heap to warm up rapidly, which significantly reduced the composting duration. At the end of composting, the water content was 53.03 %, pH was 8.1, electrical conductivity was 3.18 mS/cm, organic matter content was 46.10 % and seed germination index was 87.02 %, indicating the decomposition of the composting materials. Furthermore, CAMA significantly modified microbial community succession and increased community diversity. In total, seven major categories of biometabolic pathways were obtained by using PCRUSt2 software, and the metabolic abundance of the experimental group was higher than that of the control group, suggesting that the addition of CAMA can effectively promote faecal degradation. These findings provide novel insights into addressing the issue of human faeces accumulation in arid and cold climates.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109642"},"PeriodicalIF":3.7,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0