Biochemical Engineering Journal最新文献_第8页

Boosting of enzymatic cascades by intermediates: Theoretical analysis and model-based optimization 中间产物对酶级联的促进作用：理论分析和基于模型的优化

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-26 DOI: 10.1016/j.bej.2024.109440

引用次数: 0

Bioelectrochemical systems: Exploring microbial communities, interactions, and electron transfer 生物电化学系统：探索微生物群落、相互作用和电子传递

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-26 DOI: 10.1016/j.bej.2024.109442

{"title":"Bioelectrochemical systems: Exploring microbial communities, interactions, and electron transfer","authors":"","doi":"10.1016/j.bej.2024.109442","DOIUrl":"10.1016/j.bej.2024.109442","url":null,"abstract":"<div>Bioelectrochemical system (BES) relies on the electrochemical reactions derived from the interaction between microorganisms and solid electrodes to enable processes such as electricity generation and other biotechnological applications. The diversity of these electroactive microorganisms capable of extracellular electron transfer (EET) spread across all three domains of life. The expanding research in this domain focuses on enhancing the EET capabilities of these exoelectrogens and exploring non-exoelectrogens that can support them while reducing waste through various biogeochemical processes. Approaches such as biofilm improvement, genetic modification of electron-conducting proteins, and overexpressing redox mediators are explored to increase EET efficiency. Electrochemically inactive fermentative microorganisms that are non-exoelectrogens often coexist with exoelectrogens. Although their presence has been associated with increased power generation, their excessive proliferation can diminish power output. Therefore, understanding the synergies and intricate balance between exoelectrogens and non-exoelectrogens is necessary. This review discusses the mechanism of EET, strategies to improve EET by engineering microbial communities, the role of non-exoelectrogens involved in the BES, interactions, and synergies within microbial consortia and the factors that affect them, as well as their community structure and dynamics. This review seeks to elucidate the complex interplay within BES and pave the way for future advancements in this field by examining these aspects.</div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141841712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive review on utilizing CRISPR/Cas system for microbiome modification 关于利用 CRISPR/Cas 系统改造微生物组的全面综述

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-26 DOI: 10.1016/j.bej.2024.109443

{"title":"A comprehensive review on utilizing CRISPR/Cas system for microbiome modification","authors":"","doi":"10.1016/j.bej.2024.109443","DOIUrl":"10.1016/j.bej.2024.109443","url":null,"abstract":"<div>The recent advancements in biotechnology, particularly the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system inspired by bacterial defense mechanisms, have ushered in a new phase in gene editing. This system is now widely used for a range of purposes, including the diagnosis, treatment, and prevention of various diseases such as genetic conditions, cancer, infectious diseases, and issues related to antibiotic resistance. A particularly notable area of application is the alteration of the human gut microbiota, an essential and complex microbial ecosystem crucial for health. Imbalances in this system referred to as dysbiosis, are associated with several health issues, including heart disease, mental health disorders, and cancer. Therefore, the potential of manipulating microbiota for health benefits is significant. In accordance with this, recent research underscores the influence of modifying specific gut microbiota on the progression of diseases, notably in fields like oncology and inflammation. The current paper examines the impact of CRISPR technology in altering microbiota with the goal of exploiting its capabilities to improve human health. This exploration aims to move beyond theoretical research, applying CRISPR's sophisticated functions to complex and practical health scenarios with the ultimate objective of improving health outcomes.</div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141851829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laser wavelength and sample conditioning effects on biochemical monitoring of SARS-CoV-2 VLP production upstream stage by Raman spectroscopy 激光波长和样品调节对拉曼光谱法监测 SARS-CoV-2 VLP 生产上游阶段生化过程的影响

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-25 DOI: 10.1016/j.bej.2024.109441

{"title":"Laser wavelength and sample conditioning effects on biochemical monitoring of SARS-CoV-2 VLP production upstream stage by Raman spectroscopy","authors":"","doi":"10.1016/j.bej.2024.109441","DOIUrl":"10.1016/j.bej.2024.109441","url":null,"abstract":"<div>This work assessed the impact of laser wavelength and sample conditioning on offline monitoring (viable cell density, cell viability, virus titer, glucose, lactate, glutamine, glutamate, and ammonium) of SARS-CoV-2 virus-like particles production upstream stage by Raman spectroscopy. The evaluated chemometrics techniques were Partial Least Squares (PLS) and Artificial Neural Networks (ANN), and different spectral filtering approaches were also considered. ANN showed better prediction capacity for most of the parameters, but ammonium and lactate, better predicted by PLS, and glutamine, no difference between modeling techniques was detected. For cell growth parameters and virus titer, samples without cells measured at 785 nm Raman laser wavelength originated better-adjusted models. This laser wavelength was also more appropriate for the remaining monitored experimental parameters except for glucose, in which the best model came from the spectral database acquired at 1064 nm wavelength. Cell remotion significantly increased the accuracy of viable cell density, cell viability, glutamate, and virus titer models. However, glucose, lactate, and ammonium models showed better prediction capacity for samples containing cells. Thus, it was demonstrated that laser wavelength, sample conditioning, spectral preprocessing, and chemometric modeling techniques need to be tailored for each experimental parameter to improve accuracy.</div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141846795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering of fast growing cyanobacteria for phototrophic production of 2,3-butanediol 利用快速生长蓝藻的代谢工程进行 2,3-丁二醇的光营养生产

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-25 DOI: 10.1016/j.bej.2024.109439

{"title":"Metabolic engineering of fast growing cyanobacteria for phototrophic production of 2,3-butanediol","authors":"","doi":"10.1016/j.bej.2024.109439","DOIUrl":"10.1016/j.bej.2024.109439","url":null,"abstract":"<div>Metabolic engineering of cyanobacteria holds great potential for sustainable photosynthetic production of platform chemicals from CO2. However, product titers have been significantly low due to the slow growth rates of available model strains and lack of adequate synthetic biology tools. Here, we engineered three newly isolated fast growing Synechococcus elongatus strains PCC 11801, PCC 11802, and IITB6 for production of the platform chemical 2,3-butanediol. Importantly, we used native cyanobacterial promoters to enable inducer-free gene expression and 2,3-butanediol production. Different combinations of these native promoters were employed to optimize expression of the three-gene 2,3-butanediol synthesis pathway. Among the strains tested in this study, the highest 2,3-butanediol titer of 1.62 g L−1 (130 mg L−1 Day−1) was obtained in IITB6 (PcpcB300:alsS::PpsbAIII:alsD::PrbcL:adh), with the highest reported photosynthetic productivity in cyanobacteria cultivated on minimal media. The findings from our study highlight the potential of using fast growing S. elongatus isolates with native cyanobacterial promoters for metabolic engineering applications.</div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141842356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapamycin mitigates gas explosion-induced spleen injury in rats via mechanistic target of rapamycin (mTOR) signaling pathway 雷帕霉素通过雷帕霉素机制靶标（mTOR）信号通路减轻瓦斯爆炸诱发的大鼠脾脏损伤

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-24 DOI: 10.1016/j.bej.2024.109436

{"title":"Rapamycin mitigates gas explosion-induced spleen injury in rats via mechanistic target of rapamycin (mTOR) signaling pathway","authors":"","doi":"10.1016/j.bej.2024.109436","DOIUrl":"10.1016/j.bej.2024.109436","url":null,"abstract":"<div>Gas explosion is a recurrent event in coal mining that cause severe spleen damage due to shockwaves, which has no effective treatment. This study aimed to explore the regulatory role of autophagy in gas explosion-induced blast spleen injuries in rats. 120 Sprague-Dawley male rats were randomly divided into 4 groups, including normal control (NC), gas explosion-induced spleen injury (Model, M), autophagy inhibitor 3-methyladenine group (M+3-MA), and induction Rapamycin (RAPA) group (M+RAPA) groups. After explosion, the inhibitor group and induction group rats were immediately given intraperitoneal injection of 3-MA (15 mg/kg)/ RAPA (1 mg/kg). The rats were anesthetized and the spleen were obtained at 24 h, 72 h, and 7 days. The results showed that gas explosion reduced the spleen index, induced spleen blooding, infiltration of inflammatory cells, and increased autophagosomes. The expression of Lc3-Ⅱ was increased, whereas p62 and p-mTOR was decreased significantly (P<0.05) in model group. Compared with the model group, RAPA improved the spleen index, spleen bleeding, inflammation, and autophagosomes significantly. The expression of Lc3-Ⅱ was increased, p62 and p-mTOR was decreased significantly, but the opposite results were observed in the inhibitor group. Taken together, we firstly found that RAPA can mitigate gas explosion-induced spleen injury via mTOR signaling, which provides a new idea for the treatment of spleen injury including but not limited to coal mine accidents.</div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141840056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AI-based screening of Clostridium acetobutylicum with high furfural tolerance and butanol production 基于人工智能的高耐受糠醛和生产丁醇梭菌的筛选

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-23 DOI: 10.1016/j.bej.2024.109435

{"title":"AI-based screening of Clostridium acetobutylicum with high furfural tolerance and butanol production","authors":"","doi":"10.1016/j.bej.2024.109435","DOIUrl":"10.1016/j.bej.2024.109435","url":null,"abstract":"<div>Advances in strain breeding for butanol biosynthesis were quite limited because of physiological complexity of solventogenic Clostridia. Using AI, this study developed a high-throughput screening method for Clostridium acetobutylicum to find strains with inhibitor tolerance and high butanol production. A mutant library was generated from C. acetobutylicum ATCC 824 through ARTP mutagenesis and physiological traits were digitized using color indicators. The classification performance of Machine learning algorithms (PCA, PLS, SVM, ANN) were compared for different butanol-producing strains. Among 2000 strains screened, C. acetobutylicum Tust-f3 was identified, which could tolerate 4.5 g/L furfural and yield 10.5 g/L butanol from undetoxified lignocellulosic hydrolysate. Proteome analysis reveals that 38 proteins may play a crucial role. Subsequently, seven universal detoxification components for furfural were identified via heterologous expression in E. coli Genes CA_RS19590 and CA_RS08810 showed significant growth improvement (14.44 and 14.28-fold, respectively, compared to control). This study highlights the potential of machine learning in strain selection and breeding.</div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141839812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-stage process for mixed microbial culture production of polyhydroxyalkanoates from sugarcane stillage: Assessment of external nutrient supplementation 利用甘蔗渣混合微生物培养生产聚羟基烷酸酯的多级工艺：外部营养补充评估

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-22 DOI: 10.1016/j.bej.2024.109438

{"title":"Multi-stage process for mixed microbial culture production of polyhydroxyalkanoates from sugarcane stillage: Assessment of external nutrient supplementation","authors":"","doi":"10.1016/j.bej.2024.109438","DOIUrl":"10.1016/j.bej.2024.109438","url":null,"abstract":"<div>Sugarcane stillage is an abundant wastewater from ethanol production. It has drawn considerable interest as a potential feedstock for biotechnological processes aiming at the recovery of energy and value-added products. In this study we explored the use of stillage for the production of polyhydroxyalkanoates (PHA), which are microbial bioplastics with potential to replace conventional plastics in specific applications. As stillage is nutrient deficient, we investigated the impact of external nutrient supplementation on the selection of PHA-producing mixed cultures. Cultures selected under four different total-chemical-oxygen-demand-to-nitrogen ratios (COD.t/N) (15, 30, 40 and 80) were evaluated according to their PHA production performance and microbial composition. The experimental results demonstrated that the most favorable PHA production was achieved for the biomass selected under strict carbon-limiting conditions, with a COD.t/N ratio of 15. This resulted in an overall PHA volumetric productivity of 2.10 g PHA L−1 d−1, a maximum intracellular PHA content of 0.65 g PHA g VSS−1 and a PHA storage yield of 0.71 g CODPHA g CODH.Org−1. The resulting PHA was a copolymer of 3-hydroxybutyrate (73 %mol) and 3-hydroxyvalerate (27 %mol) monomers. The microbial consortium was enriched by members of the Brevundimonas genus, known PHA producers, with a relative abundance of 26.4 %.</div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141839158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of endoplasmic reticulum stress on reducing recombinant protein production in mammalian cells 内质网应激对降低哺乳动物细胞重组蛋白产量的作用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-20 DOI: 10.1016/j.bej.2024.109434

{"title":"The role of endoplasmic reticulum stress on reducing recombinant protein production in mammalian cells","authors":"","doi":"10.1016/j.bej.2024.109434","DOIUrl":"10.1016/j.bej.2024.109434","url":null,"abstract":"<div>Therapeutic recombinant protein production relies on industrial scale culture of mammalian cells to produce active proteins in quantities sufficient for clinical use. The combination of stresses from industrial cell culture environment and recombinant protein production can overwhelm the protein synthesis machinery in the endoplasmic reticulum (ER). This leads to a buildup of improperly folded proteins which induces ER stress. Cells respond to ER stress by activating the Unfolded Protein Response (UPR). To restore proteostasis, ER sensor proteins reduce global protein synthesis and increase chaperone protein synthesis, and if that is insufficient the proteins are degraded. If proteostasis is still not restored, apoptosis is initiated. Increasing evidence suggests crosstalk between ER proteostasis and DNA damage repair (DDR) pathways. External factors (e.g., metabolites) from the cellular environment as well as internal factors (e.g., transgene copy number) can impact genome stability. Failure to maintain genome integrity reduces cell viability and in turn protein production. This review focuses on the association between ER stress and processes that affect protein production and secretion. The processes mediated by ER stress, including inhibition of global protein translation, chaperone protein production, degradation of misfolded proteins, DNA repair, and protein secretion, impact recombinant protein production. Recombinant protein production can be reduced by ER stress through increased autophagy and protein degradation, reduced protein secretion, and reduced DDR response.</div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141845881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced removal of PHE-Cd2+ co-contamination by the mixed bacterial cultures of Pseudomonas putida and Arthrobacter sp.: Performance and mechanism 利用假单胞菌（Pseudomonas putida）和节杆菌（Arthrobacter sp：性能与机理

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-18 DOI: 10.1016/j.bej.2024.109433

{"title":"Enhanced removal of PHE-Cd2+ co-contamination by the mixed bacterial cultures of Pseudomonas putida and Arthrobacter sp.: Performance and mechanism","authors":"","doi":"10.1016/j.bej.2024.109433","DOIUrl":"10.1016/j.bej.2024.109433","url":null,"abstract":"<div>The strain species resource is essential for microbial remediation of PHE (phenanthrene)-Cd2+ co-contamination. In this study, the mixed bacterial culture (M) was established to intensively remediate PHE-Cd2+ co-contamination using PAHs-degrading bacteria Pseudomonas putida and Arthrobacter sp. with different Cd2+ tolerance. The PHE degradation efficiency of the mixed bacterial cultures (M) was 65 %-81 % under different Cd2+ concentration of 10–50 mg/L, respectively, which was 1.40–2.98 times that of the individual strains. In addition, strain metabolic enzyme activity, intracellular structure and extracellular polymeric substances (EPS) were carried out under Cd2+ stress. Results showed that the catechol 1,2-dioxygenase enzyme (C120) and the electron transport system activity (ETSA) were less adversely affected by Cd2+ in mixed bacterial cultures. Transmission electron microscopy images showed that the cells surface of the mixed bacterial cultures (M) could be adsorbed more Cd2+ compared to the single strain. The analysis of cell functional groups suggested that C-O-C and C-O groups in EPS mediated the removal of Cd2+ in the mixed bacterial culture. Moreover, the Cd2+ removal proportion by EPS adsorption of the mixed bacterial cultures accounted for 92 %, 67 %, 57 %, and 52 % under Cd2+ stress of 0.5, 10, 25, and 50 mg/L, respectively, which were superior to that of individual strains. This study confirmed the potential application and technical reference of the specific mixed bacterial cultures in the enhanced bioremediation of PHE-Cd2+ co-contamination.</div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141729234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0