Biochemical Engineering Journal最新文献_第10页

Insight into the enhanced cAMP synthesis using Arthrobacter sp. CCTCC 2013431 under polyphosphate addition condition based on physiological and transcriptome analyses 基于生理和转录组分析，深入了解在多磷酸盐添加条件下使用Arthrobacter sp. CCTCC 2013431增强cAMP合成

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-03-01 Epub Date: 2025-12-15 DOI: 10.1016/j.bej.2025.110048

Zhigang Li , Nanxun Lu , Ruifang Li , Wei Liu , Chang Li , Zhonghua Zhang , Tianyou Yang

{"title":"Insight into the enhanced cAMP synthesis using Arthrobacter sp. CCTCC 2013431 under polyphosphate addition condition based on physiological and transcriptome analyses","authors":"Zhigang Li , Nanxun Lu , Ruifang Li , Wei Liu , Chang Li , Zhonghua Zhang , Tianyou Yang","doi":"10.1016/j.bej.2025.110048","DOIUrl":"10.1016/j.bej.2025.110048","url":null,"abstract":"<div><div>The insufficient supply of ATP limited cAMP synthesis during fermentations using <em>Arthrobacter</em> sp. CCTCC 2013431. cAMP fermentations with/without hexametaphosphate addition were conducted in a 7 L bioreactor and the underlying mechanism for enhanced cAMP biosynthesis under hexametaphosphate addition condition was investigated. Due to the addition of 2 g/<span>L</span>-broth sodium hexametaphosphate at 24 h, cAMP content reached 3.64 g/L, with an increment of 33.82 % when compared with control. The transcriptome analysis results revealed that 227 genes were up-regulated and 265 genes were down-regulated significantly in response to hexametaphosphate addition. For glycometabolism, the transcription levels of key enzymes genes involved with cAMP synthesis were enhanced significantly, and for energy metabolism, the transcription levels of genes associated with electron transport chain and polyphosphate kinase gene were increased significantly by which sufficient carbon skeleton and ATP were provided for cAMP biosynthesis. In addition, transcription levels of most reductase genes were up regulated significantly suggesting that intracellular antioxidant capacity was improved effectively by hexametaphosphate. Finally, activities of key enzymes together with intracellular contents of ROS, MDA and key metabolites were detected and the results were in accordance to those of transcriptome analysis. It could be concluded that hexametaphosphate relieved severe oxidative stress, strengthened energy metabolism and redistributed the carbon flux among different pathways resulting in enhanced cAMP synthesis and decreased byproduct generation.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"227 ","pages":"Article 110048"},"PeriodicalIF":3.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrocyclone-enhanced nutrient removal and sludge densification in activated sludge system: Insights into microbial response 活性污泥系统中水力旋流器增强的营养物去除和污泥致密化：对微生物反应的见解

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-03-01 Epub Date: 2025-12-15 DOI: 10.1016/j.bej.2025.110051

Wentao Xu , Shasha Liu , Mei Sun , Shijie Sun , Jiao Wang , Zhiming Bian , Qingyun Zhang , Changsheng Peng

{"title":"Hydrocyclone-enhanced nutrient removal and sludge densification in activated sludge system: Insights into microbial response","authors":"Wentao Xu , Shasha Liu , Mei Sun , Shijie Sun , Jiao Wang , Zhiming Bian , Qingyun Zhang , Changsheng Peng","doi":"10.1016/j.bej.2025.110051","DOIUrl":"10.1016/j.bej.2025.110051","url":null,"abstract":"<div><div>Sludge densification is a promising technology to enhance nutrient removal in existing municipal wastewater treatment plants (WWTPs). In this study, the graded-device hydrocyclone was installed into activated sludge system to assist sludge densification and disclose its microbial response mechanism. Higher nutrient removal (94.2 % for COD, 97.2 % for NH<sub>4</sub><sup>+</sup>-N, 82.3 % for TN, 92.4 % for TP, respectively) were achieved in R2 reactor incorporating hydrocyclone, and these effluent indicators met the wastewater first class A discharge standards of China (GB18918–2002). The hydraulic shear forces of hydrocyclone promoted the sludge transformation from loose flocs to dense particles (average size of 292 μm), sludge settleability (relative hydrophobicity of 79.1 %, SVI<sub>30</sub> of 60 mL/g), and the secretion of extracellular polymeric substances (EPS of 98.89 mg/g-SS, protein/polysaccharide ratio of 1.78). Ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, denitrifying bacteria and polyphosphate-accumulating organisms were effectively enriched. These functional bacteria actively participated in membrane transport, signal transduction, carbon metabolism, nitrogen metabolism, phosphonate and phosphinate metabolism, providing the core driving force for long-term stable nutrient removal. These findings provided feasibility and mechanism support for hydrocyclone in enhancing nutrient removal, with a view to achieving cost reduction and efficiency benefits via sludge densification in industrial applications at full-scale WWTPs within the environmental field.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"227 ","pages":"Article 110051"},"PeriodicalIF":3.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ directed evolution of γ-glutamyl kinase in Corynebacterium glutamicum S9114 by innovative EvolvR-mediated mutagenesis and rare codon-based selection to increase L-proline production 利用创新性evolvr介导的诱变和基于罕见密码子的选择，谷氨酸棒状杆菌S9114的γ-谷氨酰基激酶原位定向进化以增加l -脯氨酸的产量

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-02-01 Epub Date: 2025-10-17 DOI: 10.1016/j.bej.2025.109982

Jian-Zhong Xu , Feng Zhang , Kai Wang , Shuo Wan , Wei-Guo Zhang

{"title":"In situ directed evolution of γ-glutamyl kinase in Corynebacterium glutamicum S9114 by innovative EvolvR-mediated mutagenesis and rare codon-based selection to increase L-proline production","authors":"Jian-Zhong Xu , Feng Zhang , Kai Wang , Shuo Wan , Wei-Guo Zhang","doi":"10.1016/j.bej.2025.109982","DOIUrl":"10.1016/j.bej.2025.109982","url":null,"abstract":"<div><div>An efficient evolution and selection system is vital for constructing the high added-value products hyper-producers in fermentation industry. Herein, an innovative EvolvR-mediated mutagenesis was used to diversify the γ-glutamyl kinase (GK)-coding gene <em>proB</em> in <em>C. glutamicum</em> S9114 and an optimized rare codon-based selection was used to isolate the L-proline high-yielding strain with optimal GK variant. Triple-mutant GK<sup>E74K/G149K/V150M</sup> was identified with high substrate affinity and catalytic efficiency for producing L-proline. The resultant strain <em>C. glutamicum</em> Pro-7 produced 94.2 ± 5.6 g/L of L-proline with productivity of 1.96 g/(L·h) and a yield of 0.316 g/g. Subsequently, the molecular mechanisms involved in high L-proline production in strain Pro-7 was discovered, indicating that ATP and NADPH supply are also the key factors for the L-proline biosynthesis besides GK. Thus, <em>in situ</em> directed evolution of key genes in genome by EvolvR-mediated mutagenesis and rare codon-based selection provides a new direction and reference to construct industrial-strength strains.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"226 ","pages":"Article 109982"},"PeriodicalIF":3.7,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145326137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Treatment of real aquaculture wastewater using marine Chlorella: Pollutant removal and microbial community dynamics 用海洋小球藻处理真实水产养殖废水：污染物去除和微生物群落动态

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-02-01 Epub Date: 2025-10-31 DOI: 10.1016/j.bej.2025.109987

Shenwei Cheng , Yanqing Sheng

{"title":"Treatment of real aquaculture wastewater using marine Chlorella: Pollutant removal and microbial community dynamics","authors":"Shenwei Cheng , Yanqing Sheng","doi":"10.1016/j.bej.2025.109987","DOIUrl":"10.1016/j.bej.2025.109987","url":null,"abstract":"<div><div>This study comprehensively investigated the nutrient removal mechanisms of <em>Chlorella</em> sp. in authentic aquaculture wastewater, while also examining the dynamic shifts within its symbiotic microbial community. The results showed that the microalgae exhibited robust growth in the wastewater, effectively reducing concentrations of ammonium nitrogen, nitrate nitrogen, and phosphate with removal efficiencies of 95.0 %, 93.2 %, and 91.7 %, respectively. Fluctuations in pH during the initial cultivation period indicated a cyclical interplay between heterotrophic microbial activity and microalgal photosynthesis. In the later stages, enhanced synthesis of <em>Chlorella</em> carotenoids was correlated with deteriorating water quality and the onset of oxidative stress. Initially, the microbial community was dominated by organic matter degradation, nitrate reduction, and fermentation. However, as cultivation progressed, metabolic activities shifted towards the photoassimilation of inorganic nutrients. The <em>Chlorella</em> sp. symbiotic system facilitated a rapid succession of the microbial community, which was characterized by frequent bacterial population changes, ultimately leading to a unique, temporally stable community structure. The initially diverse microbial population was progressively supplanted by more adaptive bacterial strains, achieving a steady state by the seventh day. Notably, <em>Alcaligenaceae</em> demonstrated exceptional adaptability compared to other genera, underscoring its pivotal role within the <em>Chlorella</em> sp. symbiotic system. This research provides valuable theoretical insights into algal-bacterial interactions and highlights their potential for application in water quality remediation.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"226 ","pages":"Article 109987"},"PeriodicalIF":3.7,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145463345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotransformation from rutin to quercetin in tomato by-products using almond β-glucosidase 利用杏仁β-葡萄糖苷酶将番茄副产品中的芦丁转化为槲皮素

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-02-01 Epub Date: 2025-10-06 DOI: 10.1016/j.bej.2025.109955

Jared Mauricio López-Téllez, María del Pilar Cañizares-Macías

{"title":"Biotransformation from rutin to quercetin in tomato by-products using almond β-glucosidase","authors":"Jared Mauricio López-Téllez, María del Pilar Cañizares-Macías","doi":"10.1016/j.bej.2025.109955","DOIUrl":"10.1016/j.bej.2025.109955","url":null,"abstract":"<div><div>Within the circular economy, quercetin is a high-value polyphenol with significant food and pharmaceutical applications, which can be chemically or enzymatically produced from rutin. This study proposes an efficient enzymatic strategy using both free and immobilized β-glucosidase for rutin hydrolysis. When standard rutin was tested hydrolysis rates reached 80.69 ± 2.89 % (free enzyme) and 82.33 ± 3.45 % (immobilized enzyme), with conversion to quercetin at 49.14 ± 2.63 % and 55.22 ± 1.90 %, respectively. Application to tomato peels and seeds showed nonetheless very promising results with rutin hydrolysis rates between 29.14 ± 2.77 % (immobilized enzyme) and 35.86 ± 3.11 % (free enzyme) and quercetin conversion from 19.31 ± 2.16 % to 21.09 ± 3.63 %. Nonetheless, quercetin content and antioxidant capacity increased closely by 32.00 % and 18.00 %, respectively, after hydrolysis. These findings introduce a rapid, sustainable, and cost-effective enzymatic process for enhancing the bioactive potential of tomato by-products, providing a valuable tool for agro-industrial applications and contributing to the valorization of food waste.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"226 ","pages":"Article 109955"},"PeriodicalIF":3.7,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145264571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiscale CFD-metabolic modeling reveals hybrid impeller configurations mitigate substrate and oxygen starvation in industrial bioreactors 多尺度cfd -代谢模型揭示了混合叶轮配置减轻了工业生物反应器中的底物和缺氧

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-02-01 Epub Date: 2025-10-08 DOI: 10.1016/j.bej.2025.109956

Qingfeng Gu , Junxiong Yu , Yongqiang Liu , Yongbo Wang , Yingping Zhuang , Chao Li

{"title":"Multiscale CFD-metabolic modeling reveals hybrid impeller configurations mitigate substrate and oxygen starvation in industrial bioreactors","authors":"Qingfeng Gu , Junxiong Yu , Yongqiang Liu , Yongbo Wang , Yingping Zhuang , Chao Li","doi":"10.1016/j.bej.2025.109956","DOIUrl":"10.1016/j.bej.2025.109956","url":null,"abstract":"<div><div>Industrial-scale aerobic fermentations face persistent challenges from spatiotemporal substrate and oxygen gradients, which impair microbial productivity during scale-up. To address this, we establish a multiscale Computational Fluid Dynamics (CFD)-metabolic modeling framework to evaluate hybrid radial-axial impeller configurations in a 156 m³ stirred tank reactor. Compared to homogeneous all-radial and all-axial systems, hybrid configurations maintain superior balance in gas-liquid mixing, mass transfer, and power consumption through synergistic flow interactions. Integrated metabolic regime analysis and Lagrangian particle tracking confirm that the hybrid radial-axial impeller configurations effectively alleviate glucose and dissolved oxygen starvation. Especially, the C-K-R-R configuration, which uses a CD-6 impeller at the bottom, an axial-flow KSX impeller on the second level, and two Rushton turbines above, demonstrates optimal performance with 41.1 % non-starvation zone coverage (3.3 times all-axial impeller configurations) and 64.9 % residence time (1.6 times all-radial impeller configurations). This work establishes a multiscale optimization framework bridging bioreactor hydrodynamics with microbial metabolism, providing actionable strategies for scaled-up bioprocess design and intensification of industrial fermentation systems.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"226 ","pages":"Article 109956"},"PeriodicalIF":3.7,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145264574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioremediation of diesel-contaminated saline soil through halotolerant-lipolytic fungal consortium and humic acid 耐盐溶脂真菌联合体和腐植酸对柴油污染盐渍土的生物修复作用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-02-01 Epub Date: 2025-10-15 DOI: 10.1016/j.bej.2025.109969

Ahmed Sarfaraz , Sumbal Sajid , Qingming Zhang , Hengxing Zhang , Lin Lin , Wenfang Chen , Farhan Nabi , Syed Muhammad Mustajab Shah , Obey Kudakwashe Zveushe , Amal Mohamed Omer , Ying Han

{"title":"Bioremediation of diesel-contaminated saline soil through halotolerant-lipolytic fungal consortium and humic acid","authors":"Ahmed Sarfaraz , Sumbal Sajid , Qingming Zhang , Hengxing Zhang , Lin Lin , Wenfang Chen , Farhan Nabi , Syed Muhammad Mustajab Shah , Obey Kudakwashe Zveushe , Amal Mohamed Omer , Ying Han","doi":"10.1016/j.bej.2025.109969","DOIUrl":"10.1016/j.bej.2025.109969","url":null,"abstract":"<div><div>Rapid urbanization has resulted in high diesel oil demand and consumption. Such processes are mostly carried out near coastal areas, where accidental oil spills occur during storage and handling, resulting in diesel-contaminated soils. Usually, soil microbial populations can remediate such contamination. However, the remediation of diesel-contaminated saline soils is challenging, as high salt concentrations inhibit the growth and activity of many hydrocarbon-degrading microbes, thereby limiting their remediation potential. In the current study, a combined approach was employed to remediate diesel-contaminated saline soil through bioaugmentation of a halotolerant-lipolytic fungal consortium (HLFC) and humic acid (HA) to alleviate the phytotoxicity of diesel and salt in <em>Lolium perenne</em> in the greenhouse experiment. The highest total petroleum hydrocarbon (TPH) degradation was achieved by the combined application of HLFC-HA (78.1 %), followed by HLFC (74.1 %), HA (65.2 %), and control (CK) (50.8 %). Plants displayed significant stress in diesel-contaminated saline soil. However, the combined application significantly reduced stress, plants showed better growth, photosynthesis, chlorophyll content, and lower antioxidant enzyme activity. Microbial diversity analysis showed that the relative abundances of the fungal genera <em>Scopulariopsis</em> and <em>Aspergillus</em> were significantly high in HLFC and HLFC-HA amended soils. Besides that, soil enzyme activity, including lipase and laccase, significantly increased in HLFC and HLFC-HA-amended soil. These findings demonstrated that HLFC-HA could be a beneficial strategy for the remediation of such oil-contaminated sites.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"226 ","pages":"Article 109969"},"PeriodicalIF":3.7,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145326138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid lactic acid fermentation of vegetable waste in liquid-phase systems 植物废弃物液相系统快速乳酸发酵研究

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-02-01 Epub Date: 2025-11-01 DOI: 10.1016/j.bej.2025.109989

Han Zhang , Xue Song , Jie Zhang , Yongde Liu , Tianrun Bai , Feiyue Wang , Guihua Yan , Longjie Xiao , Tianzeng Jin , Jihong Zhao

{"title":"Rapid lactic acid fermentation of vegetable waste in liquid-phase systems","authors":"Han Zhang , Xue Song , Jie Zhang , Yongde Liu , Tianrun Bai , Feiyue Wang , Guihua Yan , Longjie Xiao , Tianzeng Jin , Jihong Zhao","doi":"10.1016/j.bej.2025.109989","DOIUrl":"10.1016/j.bej.2025.109989","url":null,"abstract":"<div><div>In this study, vegetable waste liquid (VWL) was used as a substrate to produce lactic acid (LA) through anaerobic fermentation. The effects of inoculum size, carbon-to-nitrogen ratio (C/N ratio), temperature, and initial pH on LA yield and microbial succession were systematically investigated. All parameters significantly influenced LA production, with optimized conditions (12 % inoculum, initial pH of 6, C/N ratio of 20, 35 °C) yielding maximum LA production (11.76 g/L) within 16 h, corresponding to a 53.26 % glucose conversion rate. Excessive inoculum or deviation from optimal pH inhibited LA synthesis, whereas an appropriate C/N ratio enhanced microbial activity and substrate utilization. <em>Lactobacillus plantarum</em> was identified as the dominant species, with its relative abundance increasing from 64.14 % to 70.33 % at 16 h before stabilizing at 71.47 % at 24 h. Its metabolic dominance strongly correlated with rapid acidification, indicating its key role in efficient LA biosynthesis. Overall, precise fermentation parameter regulation effectively alleviated LA feedback inhibition and shortened the fermentation cycle to less than 24 h, offering a sustainable approach for converting vegetable waste into valuable biochemicals.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"226 ","pages":"Article 109989"},"PeriodicalIF":3.7,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145463346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temperature-tailored cellulose-rich Cladophora sp. hydrochar for selective malachite green adsorption from multi-dye systems 温度定制的富含纤维素的Cladophora sp.碳氢化合物对多染料系统中孔雀石绿的选择性吸附

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-02-01 Epub Date: 2025-10-30 DOI: 10.1016/j.bej.2025.109986

Muhammad Badaruddin , Laila Hanum , Elda Melwita , Sahrul Wibiyan , Yulizah Hanifah , Aldes Lesbani

{"title":"Temperature-tailored cellulose-rich Cladophora sp. hydrochar for selective malachite green adsorption from multi-dye systems","authors":"Muhammad Badaruddin , Laila Hanum , Elda Melwita , Sahrul Wibiyan , Yulizah Hanifah , Aldes Lesbani","doi":"10.1016/j.bej.2025.109986","DOIUrl":"10.1016/j.bej.2025.109986","url":null,"abstract":"<div><div>This study presents the selective adsorption of malachite green (MG) from multi-dye mixtures using hydrochar derived from <em>Cladophora</em> sp<em>.</em> (C) via hydrothermal carbonization (HTC) at 150°C (C150), 200°C (C200), 250°C (C250), and 300°C (C300). Structural characterizations (XRD, FTIR, SEM-EDS, BET) confirmed enhanced porosity and surface functionalization with increasing HTC temperature. C200 exhibited the highest BET surface area (15.84 m²/g), while C300, with the largest pore size (18.27 nm), showed the highest MG adsorption capacity (204.1 mg/g), outperforming C (30.03 mg/g). Selective adsorption tests using ternary anionic-cationic dye mixtures revealed strong affinity toward cationic dyes, with MG showing 95.5 % removal by C300. Kinetic studies fitted the pseudo-second-order model, suggesting chemisorption dominance; the Avrami model also correlated well. Increasing Avrami rate constant (k₃) and decreasing exponent (n) with HTC temperature indicate a transition from diffusion to surface-controlled chemisorption. Equilibrium data followed the Sips isotherm, reflecting mixed monolayer–multilayer adsorption on moderately heterogeneous surfaces. The rising Sips constant (Kₛ) with temperature confirmed an endothermic process consistent with thermodynamic findings, which also verified spontaneity. Regeneration studies showed C300 retained > 50 % efficiency after three cycles, demonstrating stability and reusability. FTIR, XRD, and XPS analyses before and after MG adsorption confirmed interactions via electrostatic attraction, hydrogen bonding, and π–π interactions.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"226 ","pages":"Article 109986"},"PeriodicalIF":3.7,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145463344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Numerical simulation of catalytic enhancement in wall-coated enzyme-catalyzed micro-channel via geometric design 基于几何设计的壁涂酶催化微通道催化强化数值模拟

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-02-01 Epub Date: 2025-10-01 DOI: 10.1016/j.bej.2025.109947

Yiruo He , Jun Liang , Huiting Xu , Zhixi Zhang , Weiyi Su , Yuqi Hu , Na Wang , Xiong Yu , Honghai Wang

{"title":"Numerical simulation of catalytic enhancement in wall-coated enzyme-catalyzed micro-channel via geometric design","authors":"Yiruo He , Jun Liang , Huiting Xu , Zhixi Zhang , Weiyi Su , Yuqi Hu , Na Wang , Xiong Yu , Honghai Wang","doi":"10.1016/j.bej.2025.109947","DOIUrl":"10.1016/j.bej.2025.109947","url":null,"abstract":"<div><div>Microfluidics is widely used in enzyme biotechnology. Wall-coated immobilized enzyme micro-channels (W-IEMRs) offer significant advantages in stability and hydrodynamic performance but have limited catalytic efficiency due to long diffusion paths in the microreactor. This study used a computational fluid dynamics (CFD) model to simulate flow fields and concentration distributions in serpentine micro-channels, quantifying mixing efficiency via the mixing index (MI) and Dean number (De). By testing channels with different curvature radius, bending ratios, and inner diameters, the geometry was optimized to enhance mass transfer and mixing. Simulations showed that a smaller curvature radius, larger bending ratio, and smaller inner diameter strengthen Dean vortices, improving mixing. However, catalytic efficiency has a non-monotonic relationship with these parameters: a curvature radius below 6.31 mm reduces enzyme-substrate contact due to excessive vortices, while a bending ratio exceeding 60 % leads to uneven substrate distribution caused by counter-rotating vortices in adjacent sections, impairing performance. Optimal design parameters are: curvature radius 6.31 mm, bending ratio 60 %, inner diameter 0.5 mm. For micro-channels with varying reaction kinetics, careful consideration must be given to balancing mass transfer and catalytic performance.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"226 ","pages":"Article 109947"},"PeriodicalIF":3.7,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145218879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0