Biochemical Engineering Journal最新文献_第10页

Enhanced degradation of petroleum hydrocarbons by immobilizing Acinetobacter 固定化不动杆菌促进石油烃的降解

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-07 DOI: 10.1016/j.bej.2025.109666

Jienan Li , Hua Zhang , Kuixi Mei , Leni Sun , Li Wang , Changhai Liang

{"title":"Enhanced degradation of petroleum hydrocarbons by immobilizing Acinetobacter","authors":"Jienan Li , Hua Zhang , Kuixi Mei , Leni Sun , Li Wang , Changhai Liang","doi":"10.1016/j.bej.2025.109666","DOIUrl":"10.1016/j.bej.2025.109666","url":null,"abstract":"<div><div>The highly efficient elimination of petroleum pollution is essential for addressing environmental issues and social sustainability. A hydrocarbon-degrading bacterium SHC is isolated from petroleum-contaminated soil in Chengdu, China, to augment bioremediation efforts against crude oil contamination. According to the analysis of 16S rDNA sequences, strain SHC was identified as <em>Acinetobacter</em>. The degradation rate of 2 g/L crude oil by free bacterium reached 30 % within 7 days. Through the analysis of hydrocarbon biodegradation, strain SHC was found to biodegrade more long-chain n-alkanes (C19–C29). Further, we delve into utilizing agar as a microbial immobilization matrix, supplemented with nutrient salts and oil-absorbing materials, to foster the growth and activity of the bacterium in challenging environments characterized by high salinity and acidity. In the final immobilization bacterium, the degradation rate of 3 % salt concentration reaches 22 %, the degradation rate of 5 g/L crude oil is 45 %, and the degradation rate at a pH of 10 is 74 %, and was 59 % more effective than the free bacterium on the removal of crude oil under the same conditions. These findings strongly indicate that encapsulating <em>Acinetobacter</em> SHC can protect them against extreme conditions, bolstering bioremediation endeavors.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109666"},"PeriodicalIF":3.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143436723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements of the Vibrio coralliilyticus eDNA detection based on Co-Fe PBA-assisted biosensors for the rapid coral-disease warning 基于Co-Fe - pba辅助生物传感器的珊瑚弧菌eDNA检测研究进展

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-06 DOI: 10.1016/j.bej.2025.109662

Yibo Zhang , Hongjie Liu , Shaopeng Wang , Hao Fu , Yuanyu Xie , Chaoxin Zhang , Man Zhang , Jie Lu , Liwei Wang , Kefu Yu

{"title":"Advancements of the Vibrio coralliilyticus eDNA detection based on Co-Fe PBA-assisted biosensors for the rapid coral-disease warning","authors":"Yibo Zhang , Hongjie Liu , Shaopeng Wang , Hao Fu , Yuanyu Xie , Chaoxin Zhang , Man Zhang , Jie Lu , Liwei Wang , Kefu Yu","doi":"10.1016/j.bej.2025.109662","DOIUrl":"10.1016/j.bej.2025.109662","url":null,"abstract":"<div><div><em>Vibrio coralliilyticus</em> (<em>V. coralliilyticus</em>) can lead to severe coral diseases and is one of the main causes of coral bleaching. However, there is still a lack of convenient, and non-invasive detection methods for <em>V. coralliilyticus</em>, which brings inconvenience to coral health management. Here, a portable electrochemical biosensor was developed based on environmental DNA (eDNA) and cobalt-iron Prussian Blue Analogue (Co-Fe PBA) electrode material for detecting <em>V. coralliilyticus</em>. Notably, this sensor identified and quantified <em>V. coralliilyticus</em> eDNA in water through specific DNA probes, overcoming the previously reported complex and destructive pretreatment processes. Additionally, thanks to the high specific surface area of Co-Fe PBA, which provided abundant sites for probe anchoring, the constructed portable biosensor exhibited excellent detection performances. Specifically, the detection limit was as low as 19.0 fM, and the linear range was from 100 fM to 100 nM. In subsequent coral infection experiments, the eDNA of <em>V. coralliilyticus</em> increased on the eve of coral bleaching. Based on this change, the occurrence of coral bleaching was successfully predicted. Therefore, this portable biosensor is suitable for dynamic monitoring of <em>V. coralliilyticus</em>, helping to reveal the potential risks to coral health and providing a reference for ecological decision-making for coral reef protection.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109662"},"PeriodicalIF":3.7,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing waste management and nutrient recovery: Preparation of N slow-release fertilizer using sewage sludge and its release behavior, effects on ryegrass (Lolium perenne L.) growth 加强废物管理和养分回收：利用污水污泥制备氮缓释肥料及其释放行为，对黑麦草生长的影响

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-06 DOI: 10.1016/j.bej.2025.109664

Diannan Huang , Yu Gao , Li Zhang , Rongxin Zhang , Yuhan Wu , Hao Guan , Di Luo

{"title":"Enhancing waste management and nutrient recovery: Preparation of N slow-release fertilizer using sewage sludge and its release behavior, effects on ryegrass (Lolium perenne L.) growth","authors":"Diannan Huang , Yu Gao , Li Zhang , Rongxin Zhang , Yuhan Wu , Hao Guan , Di Luo","doi":"10.1016/j.bej.2025.109664","DOIUrl":"10.1016/j.bej.2025.109664","url":null,"abstract":"<div><div>Efficient recovery and utilization of the nitrogen(N) contained in sewage sludge (SS) is a key consideration in the treatment of SS. In this study, a novel sludge hydrogel sphere slow-release N fertilizer was prepared based on the N-rich property of SS, using SS as a substrate and Fe<sup>3+</sup>(extracted from drinking water sludge (DWTS)), CMC, and ATP synergistically constructed as an encapsulated skeletal material. Based on the comprehensive consideration of hydrogel sphere formation performance, mechanical strength and encapsulation effect, the optimum mass ratio for the preparation of sludge hydrogel sphere was: M<sub>SS</sub>: M<sub>CMC</sub>: M<sub>HATP</sub>: M<sub>DWTS</sub> = 300: 4: 6:0.07; the concentration of HCl-modified ATP was 2.25 mol/L, and the number of coating layers was 1. The cumulative N release rates increased gradually with increasing soil moisture content, temperature and pH. The Korsmeyer-Peppas model can well describe the N release kinetic from sludge hydrogel spheres (R<sup>2</sup> = 0.9509). Potting experiments showed that the sludge hydrogel spheres not only promoted ryegrass growth, but also improved plant quality. The N retention mechanism of sludge hydrogel spheres mainly includes the physical barrier of coating and the adsorption of NH<sub>4</sub><sup>+</sup> by electronegative electrostatic gravitational force of hydrogel spheres. And the degradation and rupture of the coating in the later stage guaranteed the N release. This study provides new ideas for the preparation of slow-release fertilizers from solid waste resource utilization.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109664"},"PeriodicalIF":3.7,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel aquaculture wastewater treatment by an efficient nitrogen-removing bacterium capable of simultaneous nitrification and denitrification 新型水产养殖废水处理的高效脱氮细菌能够同时硝化和反硝化

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-05 DOI: 10.1016/j.bej.2025.109663

Yuhang Song , Lingmin Zhao , Lixing Huang , Yingxue Qin , Jiaonan Zhang , Jiaoling Zhang , Qingpi Yan

{"title":"Novel aquaculture wastewater treatment by an efficient nitrogen-removing bacterium capable of simultaneous nitrification and denitrification","authors":"Yuhang Song , Lingmin Zhao , Lixing Huang , Yingxue Qin , Jiaonan Zhang , Jiaoling Zhang , Qingpi Yan","doi":"10.1016/j.bej.2025.109663","DOIUrl":"10.1016/j.bej.2025.109663","url":null,"abstract":"<div><div>Nitrogen pollution from aquaculture effluent poses a severe threat to aquatic ecosystems, necessitating efficient treatment solutions. In this study, a highly effective nitrogen-removing bacterium, designated SA20, was isolated from an aquaculture wastewater treatment system and identified as <em>Paracoccus</em> sp. Safety evaluations confirmed its non-hemolytic nature and broad antibiotic sensitivity, indicating its potential for safe application. Under optimized conditions (C/N ratio 16, 30 ℃, 140 rpm), the strain demonstrated complete removal of ammonium and nitrite, with over 95 % nitrate removal within 24 h. Nitrogen balance analysis revealed that approximately 70–80 % of total nitrogen was converted to gaseous form through denitrification, while 20–30 % was assimilated into biomass. When applied to actual aquaculture wastewater, SA20 achieved significant removal of various nitrogen forms, with total nitrogen removal exceeding 65 %. These findings demonstrate the potential of strain SA20 for practical application in aquaculture wastewater treatment, offering a promising biotechnology solution for sustainable aquaculture development.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109663"},"PeriodicalIF":3.7,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143335202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of defective UiO-67 for CA immobilization to improve the ability of CO2 capture and conversion 用于CA固定的缺陷UiO-67的制备，以提高CO2捕获和转化能力

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-01-31 DOI: 10.1016/j.bej.2025.109661

Rui Zhao, Wenhui Yang, Pengyan Yang, Yunhan Cao, Fenghuan Wang

{"title":"Preparation of defective UiO-67 for CA immobilization to improve the ability of CO2 capture and conversion","authors":"Rui Zhao, Wenhui Yang, Pengyan Yang, Yunhan Cao, Fenghuan Wang","doi":"10.1016/j.bej.2025.109661","DOIUrl":"10.1016/j.bej.2025.109661","url":null,"abstract":"<div><div>Carbonic anhydrase (CA) has been suggested as a biocatalyst for carbon dioxide capture since it could effectively catalyze the hydration of carbon dioxide. Metal-organic frameworks (MOFs) have attracted significant attention in the development of immobilized enzyme carriers due to their pore tunability and excellent stability. In this study, UiO-67 materials with varying defect degrees (DUiO-67) were prepared using the organic linker missing method to enhance the catalytic performance. The results indicated that both the defect degree and the pore size of DUiO-67 materials decreased with an increase in acetic acid equivalent. After CA immobilization, all DUiO-67 materials exhibited excellent thermal, pH and recycling stability. CA@DUiO-67–25 showed higher enzyme catalytic activity and conversion ability than the other materials. After 10 cycles, CA@DUiO-67–25 retained 57 % of its initial conversion capacity. The construction of defective MOFs provides a promising new strategy for CA immobilization to achieve the catalytic conversion of CO<sub>2</sub>.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109661"},"PeriodicalIF":3.7,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly selective detection of heavy metal ions in food and water using a 5-BHAHS@NC/MnO2-based electrochemical sensor 利用5-BHAHS@NC/ mno2基电化学传感器对食物和水中重金属离子进行高选择性检测

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-01-30 DOI: 10.1016/j.bej.2025.109660

Amany El Sikaily , Doaa Gh. Ghoniem , Omar Ramadan , Eslam M. El-Nahrery , Ahmed Shahat , Rabeay Y.A. Hassan

{"title":"Highly selective detection of heavy metal ions in food and water using a 5-BHAHS@NC/MnO2-based electrochemical sensor","authors":"Amany El Sikaily , Doaa Gh. Ghoniem , Omar Ramadan , Eslam M. El-Nahrery , Ahmed Shahat , Rabeay Y.A. Hassan","doi":"10.1016/j.bej.2025.109660","DOIUrl":"10.1016/j.bej.2025.109660","url":null,"abstract":"<div><div>Human health and food safety face significant risks due to heavy metal contamination in food and water, as these metals can be accumulated to exhibit high toxicity even at low concentrations. Thus, electrochemical sensors, specifically voltammetry, provide a promising approach for simultaneous detection of heavy metal ions. In this study, a voltammetric assay was developed by directly immobilizing a newly synthesized ionophore structure, (<em>E</em>)-4-((5-bromo-2-hydroxybenzylidene) amino)-3-hydroxynaphthalene-1-sulfonic acid) (5-BHAHS), onto nano-cellulose (NC) synthesized via green methods, along with MnO<sub>2</sub> nanoparticles. The sensing material and its parent nano-cellulose base were characterized using HR-TEM, FT-IR, FE-SEM, XPS, and XRD. Subsequently, various factors influencing metal ion detection were studied and optimized, resulting in detection limits of 0.12 µM for both Hg²⁺ and Cd²⁺, and 0.03 µM for Pb²⁺. Optimal conditions for accumulation time, electrolyte pH, scan rate, selectivity, and sensitivity were determined to achieve rapid simultaneous detection at low concentrations. The 5-BHAHS@NC/MnO<sub>2</sub>-based sensor proved to be a robust tool for the simultaneous detecting Hg²⁺, Cd²⁺, and Pb²⁺ ions in tap water, wastewater, and fish samples demonstrating high selectivity. Notably, the sensitivity achieved by the 5-BHAHS@NC/MnO₂ sensor is higher than that reported in previous studies, highlighting its superior performance, non-toxicity feedback and potential for practical applications in food safety and environmental monitoring.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109660"},"PeriodicalIF":3.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-step solvothermal preparation of Fe3O4@HA magnetic nanomicrospheres for simultaneous removal of tetracycline antibiotics 一步溶剂热法制备Fe3O4@HA磁性纳米微球同时去除四环素类抗生素

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-01-29 DOI: 10.1016/j.bej.2025.109651

Sisi Li , Shuang Li , Xiang Li , Xiaodan Zeng , Jie Chen , Zhigang Liu , Shihua Yu , Junjing Hao

{"title":"One-step solvothermal preparation of Fe3O4@HA magnetic nanomicrospheres for simultaneous removal of tetracycline antibiotics","authors":"Sisi Li , Shuang Li , Xiang Li , Xiaodan Zeng , Jie Chen , Zhigang Liu , Shihua Yu , Junjing Hao","doi":"10.1016/j.bej.2025.109651","DOIUrl":"10.1016/j.bej.2025.109651","url":null,"abstract":"<div><div>Humic acid (HA) is a naturally biomass, that is considered as an ideal adsorbent material due to the abundance of functional groups on its surface. For this purpose, the highly dispersible magnetic humic acid (Fe<sub>3</sub>O<sub>4</sub>@HA) has been prepared by one-step solvothermal method. The developed Fe<sub>3</sub>O<sub>4</sub>@HA not only has a strong separation, but also can simultaneously adsorb tetracycline antibiotics (tetracycline, chlortetracycline hydrochloride and doxycycline hydrochloride). Batch sorption experiments convinced that the maximum tetracycline antibiotics adsorption capacity of Fe<sub>3</sub>O<sub>4</sub>@HA is 45.58, 86.87, and 88.46 mg/g and the largest removal of 94.5 %, 96.3 %, and 97.4 %, fast equilibrium time of 60 min. Meanwhile, the adsorption process is agreement with pseudo-second-order adsorption kinetics and Langmuir adsorption isotherm model. Thermodynamic data indicate that this adsorption is endothermic and spontaneous. Moreover, the designed Fe<sub>3</sub>O<sub>4</sub>@HA with the characteristic of anti-interference capabilities, a variety of water-matrix suitability and maintaining at least 80 % tetracycline antibiotics removal after five adsorption cycles. Finally, the adsorption mechanisms are explored by XPS and FT-IR analysis, which show that mainly involve electrostatic adsorption; hydrogen bonding, π–π, oxygen-containing functional group interactions; and physical adsorption on the surface. In conclusion, Fe<sub>3</sub>O<sub>4</sub>@HA demonstrates considerable promise as an adsorbent for treating antibiotic-contaminated water.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109651"},"PeriodicalIF":3.7,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143335201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance and phosphorus release of an anaerobic co-digestion of excess sludge and food waste containing garlic at varying substrate ratio 不同底物比下含大蒜的剩余污泥和食物垃圾厌氧共消化的性能和磷释放

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-01-29 DOI: 10.1016/j.bej.2025.109659

Lixin Wei , Yuchen Wang , Yuan He , Guangrong Zhou , Shutong Dong , Yongan Chen , Qing Wang , Yuxiang Lu , Chengyuan Su

{"title":"Performance and phosphorus release of an anaerobic co-digestion of excess sludge and food waste containing garlic at varying substrate ratio","authors":"Lixin Wei , Yuchen Wang , Yuan He , Guangrong Zhou , Shutong Dong , Yongan Chen , Qing Wang , Yuxiang Lu , Chengyuan Su","doi":"10.1016/j.bej.2025.109659","DOIUrl":"10.1016/j.bej.2025.109659","url":null,"abstract":"<div><div>The effects of different ratios of food waste (FW) containing garlic and excess sludge (ES) on the anaerobic co-digestion (AcoD) process were investigated. This was done by establishing a sequencing batch anaerobic digester and analyzing phosphorus release patterns, microbial community structure and gene function in the AcoD system with 1:1, 1:2, 1:4, and 1:8 ratio of FW to ES. The highest phosphine (PH<sub>3</sub>) gas output of 1619.99 mg/m<sup>3</sup> was observed when FW:ES was 1:1. The relative abundance of <em>Methanothrix</em> increased from 7.99 % to 9.36 % with variation the ratio of FW:ES from 1:4–1:8. The contents of <em>phnD</em> and <em>phnE</em> genes in the ratio of 1:4 FW:ES group were the highest among all experimental groups, with 0.0010 % and 0.0018 %, respectively. In addition, the abundance of functional genes responsible for encoding acetate kinase in the 1:4 FW:ES group increased by 152.96 % relative to the control group. Tests determined the optimal FW to ES ratios to achieve 1:4 and 1:8, and provided suitable operating conditions and references for the subsequent AcoD research.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109659"},"PeriodicalIF":3.7,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable production of plant biostimulants from cephalosporin fermentation residues: Ultrasonic dissolution and enzymatic hydrolysis 从头孢菌素发酵残留物中可持续生产植物生物刺激素：超声波溶解和酶水解

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-01-28 DOI: 10.1016/j.bej.2025.109658

Dongze Niu , Peiwen Ding , Wenying An , Chunyu Li , Dongmin Yin , Taoli Huhe , Jianjun Ren

{"title":"Sustainable production of plant biostimulants from cephalosporin fermentation residues: Ultrasonic dissolution and enzymatic hydrolysis","authors":"Dongze Niu , Peiwen Ding , Wenying An , Chunyu Li , Dongmin Yin , Taoli Huhe , Jianjun Ren","doi":"10.1016/j.bej.2025.109658","DOIUrl":"10.1016/j.bej.2025.109658","url":null,"abstract":"<div><div>Cephalosporin fermentation residues (CFR) is one of the most challenging industrial organic wastes due to their high nitrogen content. To transform the waste into high-value products, this study explored the effects of various factors on the solubility of total nitrogen (TN) and amino acids (AAs) and the enzymatic hydrolysis characteristics of proteins in CFR and evaluated the bioactivity of the hydrolysates through soybean germination experiments. Results showed that the solubility of AAs and TN reached their peaks at pH = 12 and 50℃, and ultrasonic treatment for 4 h further improved their dissolution. After 24 hours of hydrolysis, the AA yield in the group combining ultrasonication and enzymatic hydrolysis reached 217.5 g/kg dry matter, an 8 % increase compared to the control. The contents of some AAs, such as glutamic acid, phenylalanine, histidine, and arginine, increased by 43–85 % in the hydrolysate. Fluorescence spectroscopy confirmed the release of aromatic amino acids, such as tryptophan and tyrosine, which are known to improve plant stress tolerance. Furthermore, the diluted hydrolysates significantly promoted soybean seed germination under cold and saline conditions (<em>P</em> < 0.05), with the highest germination rates observed at a 1000x dilution. These findings highlight the effectiveness of enzymatic hydrolysis in transforming CFR into plant biostimulants, offering a sustainable solution for agricultural and environmental applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109658"},"PeriodicalIF":3.7,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving the fermentability of dilute acid hydrolysate and recovering low-concentration p-toluenesulfonic acid based on a fixed-bed column with hyper-cross-linked resin 利用超交联树脂固定床柱提高稀酸水解物发酵性能，回收低浓度对甲苯磺酸

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-01-27 DOI: 10.1016/j.bej.2025.109657

Junjie Hao , Hailong Li , Xuefang Chen , Zhijie Shen , Chaoqin Zhang , Zhen Xiong , Can Wang , Haijun Guo , Hairong Zhang , Lian Xiong , Sansan Yu , Xinde Chen

{"title":"Improving the fermentability of dilute acid hydrolysate and recovering low-concentration p-toluenesulfonic acid based on a fixed-bed column with hyper-cross-linked resin","authors":"Junjie Hao , Hailong Li , Xuefang Chen , Zhijie Shen , Chaoqin Zhang , Zhen Xiong , Can Wang , Haijun Guo , Hairong Zhang , Lian Xiong , Sansan Yu , Xinde Chen","doi":"10.1016/j.bej.2025.109657","DOIUrl":"10.1016/j.bej.2025.109657","url":null,"abstract":"<div><div>As the closest pretreatment to industrialization, dilute acid hydrolysis is effective in producing fermentable sugars from lignocellulose. However, the reuse of acid catalysts is a difficult problem that restricts the economy of the biorefining process. In addition, inhibitors generated during dilute acid pretreatment seriously affect the bioconversion of fermentable sugars. In this study, the dynamic adsorption and desorption of dilute acid hydrolysate on a fixed-bed column with hyper-cross-linked resin were investigated to improve the fermentability of hydrolysate by removing inhibitors and recover the low-concentration <em>p</em>-toluenesulfonic acid (<em>p</em>-TsOH) for cyclic pretreatment. The results showed that <em>p</em>-TsOH can be recovered by dynamic adsorption and desorption for cyclic pretreatment, which released similar concentration of xylose compared with the first pretreatment. Moreover, the total sugar loss rate was less than 2 % after adsorption. The fermentability of hydrolysate after adsorption by the fixed-bed column was greatly improved. The fermentation performance of <em>Trichosporon cutaneum</em> (<em>T. cutaneum</em>) was enhanced with a yeast biomass of 19.26 g/L, lipid content of 25.62 %, and lipid yield of 4.93 g/L. Compared with the fermentation results of hydrolysate without detoxification, the lipid content and lipid yield increased by 67.12 % and 94.10 %, respectively. In summary, this study provides a feasible technology for improving the fermentability of dilute acid hydrolysate and recovering acid catalysts.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109657"},"PeriodicalIF":3.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0