Biochemical Engineering Journal最新文献_第9页

Investigating the role of covalent stapling and cavity filling in enzyme thermostability 探讨共价钉接和空腔填充在酶热稳定性中的作用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-03-01 Epub Date: 2025-11-26 DOI: 10.1016/j.bej.2025.110026

Guojuan Yi , Zidan Liu , Linyu Luo , Zhiqiang Ding , Tolbert Osire , Mengfei Long , Yongmei Xie

{"title":"Investigating the role of covalent stapling and cavity filling in enzyme thermostability","authors":"Guojuan Yi , Zidan Liu , Linyu Luo , Zhiqiang Ding , Tolbert Osire , Mengfei Long , Yongmei Xie","doi":"10.1016/j.bej.2025.110026","DOIUrl":"10.1016/j.bej.2025.110026","url":null,"abstract":"<div><div>The enhancement of enzyme thermostability is critical for industrial applications such as food processing, and covalent bond engineering, such as disulfide bond formation, has proven effective in achieving this goal. However, the relationship between disulfide bond engineering and internal cavity modulation remained unclear. In this study, we first optimized the thermostability and catalytic performance of pullulanase using computer-aided disulfide bond engineering. The double disulfide bond mutant S643C-E668C/R680C-H715C exhibited significant improvements in thermostability (<em>T</em><sub>m</sub> increased by 6℃, half-life at 70℃ extended by 1.64-fold) and catalytic efficiency (specific activity enhanced by 21.5 %). Molecular dynamics simulations revealed that disulfide bonds stabilized the enzyme structure by reducing conformational flexibility, increasing molecular compactness, and optimizing internal cavities. Building on these findings, we developed a covalent bond “stapling” strategy based on internal cavity engineering and applied it to ethyl carbamate (EC) hydrolase, incorporating both disulfide and non-natural thioether bonds. Specific mutants, such as M46C-K123C and I76C-L212TAG, significantly improved catalytic activity, ethanol tolerance, and thermostability. These enhancements were attributed to rigid connections formed by covalent bonds in critical regions, which mitigated local stress or conformational changes potentially induced by cavity-filling mutations. By elucidating the synergistic effects of disulfide bond engineering and cavity filling, this study provided a novel theoretical and practical foundation for designing industrial enzymes with superior thermostability.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"227 ","pages":"Article 110026"},"PeriodicalIF":3.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145617580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing ammonium supply strategies for mitigation of free ammonia inhibition in the mass cultivation of Limnospira fusiformis 优化铵供应策略以缓解梭形Limnospira fususiformis大规模养殖中游离氨抑制

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-03-01 Epub Date: 2025-11-26 DOI: 10.1016/j.bej.2025.110018

Haymanot Yenesew Sewunet , Anupreet Kaur Chowdhary , Yuanjun Xia , Mutsumi Sekine , Pranshu Bhatia , Ayirkm Adugna Woldie , Tatsuki Toda

{"title":"Optimizing ammonium supply strategies for mitigation of free ammonia inhibition in the mass cultivation of Limnospira fusiformis","authors":"Haymanot Yenesew Sewunet , Anupreet Kaur Chowdhary , Yuanjun Xia , Mutsumi Sekine , Pranshu Bhatia , Ayirkm Adugna Woldie , Tatsuki Toda","doi":"10.1016/j.bej.2025.110018","DOIUrl":"10.1016/j.bej.2025.110018","url":null,"abstract":"<div><div>Ammonium is abundant in agro-industrial effluents, providing a cost-effective and sustainable nitrogen source for cultivating the cyanobacterium <em>Limnospira fusiformis</em>. However, under high pH conditions, ammonium converts into free ammonia (NH<sub>3</sub>), causing inhibition. Therefore, an optimal ammonium supply strategy is required. This study assessed <em>L. fusiformis</em> growth under intermittent, continuous, and nighttime ammonium supply methods across four nitrogen loading rates: 3.9, 5.8, 11.6, and 17.4 mg NH₄⁺-N L⁻¹ d⁻¹ . At a loading rate of 11.6 mg NH₄⁺-N L⁻¹ d⁻¹ , intermittent and continuous supply yielded higher productivities (0.22 and 0.20 g L⁻¹ d⁻¹, respectively) than the nighttime method (0.15 g L⁻¹ d⁻¹) (<em>p</em> < 0.05). Increasing the loading rate to 17.4 mg NH₄⁺-N L⁻¹ d⁻¹ resulted in elevated NH₃ concentrations, leading to growth inhibition and productivity declines to 0.09, 0.10, and 0.06 g L⁻¹ d⁻¹ under intermittent, continuous, and nighttime supply, respectively (<em>p</em> < 0.05). This study is the first to demonstrate that continuous or intermittent ammonium supply strategies can be effectively applied in the mass cultivation of <em>L. fusiformis</em>.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"227 ","pages":"Article 110018"},"PeriodicalIF":3.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145682006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bamboo biochar boosts methane production, microbial resilience, and economic performance in ammonia-rich anaerobic digestion 竹制生物炭促进甲烷产量、微生物恢复力和富氨厌氧消化的经济性能

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-03-01 Epub Date: 2025-11-20 DOI: 10.1016/j.bej.2025.110008

Kazutaka Ueno , Gen Yoshida , Mohamed Farghali , Masahiro Iwasaki , Dalia Hassan , Ikko Ihara

{"title":"Bamboo biochar boosts methane production, microbial resilience, and economic performance in ammonia-rich anaerobic digestion","authors":"Kazutaka Ueno , Gen Yoshida , Mohamed Farghali , Masahiro Iwasaki , Dalia Hassan , Ikko Ihara","doi":"10.1016/j.bej.2025.110008","DOIUrl":"10.1016/j.bej.2025.110008","url":null,"abstract":"<div><div>Anaerobic digestion (AD) is a promising technology for converting organic waste into renewable energy, but its industrial implementation is often constrained by ammonia inhibition in nitrogen-rich feedstocks, which undermines both process stability and economic viability. Addressing this challenge is crucial for ensuring sustainable, financially resilient waste-to-energy systems. We hypothesized that the strategic addition of bamboo biochar (BBC) could mitigate ammonia stress while promoting a more robust microbial community, thereby enhancing both environmental and economic performance. To test this, batch experiments were conducted to determine optimum BBC dosages, followed by semi-continuous trials using 6.25 g/L BBC over four operational phases (Runs1–4), during which NH₄⁺-N was gradually increased from 2000 to 5000 mg/L. The biochar-amended system maintained stable performance under conditions that caused control reactors to fail, with a maximum 1447 % increase in methane production observed during the 4000 mg/L NH₄⁺-N phase. Mechanistic analysis revealed that BBC acted primarily by enriching syntrophic bacteria and hydrogenotrophic methanogens, enabling a stable syntrophic acetate oxidation pathway. Enhancing microbial resilience through biochar addition directly improves financial stability, a critical factor for industrial adoption. The biochar-added system achieved consistent profits of USD 8.08–16.27/m<sup>3</sup> reactor/month, underscoring strong business potential in scalable waste-to-energy systems. Optimizing biochar dosing and evaluating full-scale implementation could further advance globally relevant, economically viable circular bioeconomy solutions.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"227 ","pages":"Article 110008"},"PeriodicalIF":3.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145682447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable bioelectricity production in wetland-microbial fuel cells: The role of carbon-based wire and Echinodorus cordifolius as a nutrient source 湿地微生物燃料电池的可持续生物电生产：碳基金属丝和棘毛虫作为营养源的作用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-03-01 Epub Date: 2025-12-02 DOI: 10.1016/j.bej.2025.110036

Azizuddin Muhammad Nashafi , Rujira Dolphen , Sucheewin Krobthong , Yodying Yingchutrakul , Chairat Treesubsuntorn

{"title":"Sustainable bioelectricity production in wetland-microbial fuel cells: The role of carbon-based wire and Echinodorus cordifolius as a nutrient source","authors":"Azizuddin Muhammad Nashafi , Rujira Dolphen , Sucheewin Krobthong , Yodying Yingchutrakul , Chairat Treesubsuntorn","doi":"10.1016/j.bej.2025.110036","DOIUrl":"10.1016/j.bej.2025.110036","url":null,"abstract":"<div><div>Achieving stable bioelectricity production in wetland-microbial fuel cells (WMFCs) remains challenging due to material degradation and fluctuating environmental conditions. This study investigates the long-term performance of carbon-based electrodes and wires in WMFC systems by assessing cathodic physiochemical properties and rhizosphere metabolomics under light (700 μmol·m⁻²·s⁻¹) and dark conditions. Over 150 days, carbon-based wire systems generated 3.7 times higher bioelectricity than commercial copper-based wires. By the final day, the Plant + Carbon wire system achieved a power density of 31.71 ± 7.11 mW/m², compared to 8.59 ± 5.35 mW/m² in the Plant + Copper wire system. Light intensity and cathodic temperature strongly influenced bioelectricity, with higher generation during the light period (8.28 ± 2.93–12.29 ± 5.56 mW/m²) than in darkness (7.08 ± 3.27–7.15 ± 4.26 mW/m²). Interestingly, planted systems consistently exhibited more stable power generation than unplanted systems, likely due to enhanced rhizosphere activity and distinctive metabolite profiles that supported electron transfer and temperature adaptation. Metabolomic analysis revealed up-regulated metabolites, including 10-undecenoic acid and carnitine derivatives, which may function as nutrients, electron acceptors, and thermoprotectants under diurnal temperature fluctuations. These findings highlight the role of wetland plants and carbon-based materials in improving WMFC resilience, ensuring operational stability, and enabling long-term bioelectricity generation.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"227 ","pages":"Article 110036"},"PeriodicalIF":3.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145682005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative evaluation of a hybrid membrane treatment train versus full-scale aerobic/anoxic activated sludge system: C-N removal performances, syntrophic microbial dynamics, and energy savings 混合膜处理系统与全规模好氧/缺氧活性污泥系统的比较评价：C-N去除性能、共生微生物动力学和节能

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-03-01 Epub Date: 2025-12-16 DOI: 10.1016/j.bej.2025.110052

Santhana Krishnan , Araya Thongsai , Pongsak (Lek) Noophan , Muhammad Ahmar Siddiqui , Yee-Shian Wong , Jaeho Bae , Sumate Chaiprapat

{"title":"Comparative evaluation of a hybrid membrane treatment train versus full-scale aerobic/anoxic activated sludge system: C-N removal performances, syntrophic microbial dynamics, and energy savings","authors":"Santhana Krishnan , Araya Thongsai , Pongsak (Lek) Noophan , Muhammad Ahmar Siddiqui , Yee-Shian Wong , Jaeho Bae , Sumate Chaiprapat","doi":"10.1016/j.bej.2025.110052","DOIUrl":"10.1016/j.bej.2025.110052","url":null,"abstract":"<div><div>This study presents a novel hybrid membrane treatment train that integrates an anaerobic membrane bioreactor (AnMBR) with a fluidized bed membrane bioreactor enriched with partial nitritation and anammox (FMBR<sup>PN/A</sup>) for the treatment of industrial wastewater. The system was evaluated in terms of pollutant removal performance, microbial community structure, and energy efficiency, and benchmarked against a conventional full-scale aerobic/anoxic activated sludge system with an integrated constructed wetland (AS<sup>N/DN</sup>+Wetland). The AnMBR+FMBR<sup>PN/A</sup> achieved high removal efficiencies for COD (91.1 %) and total nitrogen (70.1 %), matching the performance of the conventional system while operating under low-carbon effluent conditions. Strategic aeration of FMBR<sup>PN/A</sup> enabled spatial separation of partial nitritation and anammox zones, promoting syntrophic coexistence of ammonium-oxidizing (AOB), nitrite-oxidizing (NOB), denitrifying (DN), and anammox (AMX) microbes in direct contact with the membrane surface, contrasted with microbial dynamics in traditional systems. The AnMBR+FMBR<sup>PN/A</sup> process had better energy performance, with 26.6 % reduction in electricity consumption (0.55 vs. 0.74 kWh/m<sup>3</sup>) and added benefits in biomethane recovery and reduced greenhouse gas emissions. These findings point to the potential of hybrid membrane bioreactors to transform industrial nitrogen management towards more efficient and sustainable solutions.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"227 ","pages":"Article 110052"},"PeriodicalIF":3.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of high-temperature fermentation liquid and mechanically dewatering liquid on algal-bacterial granular sludge: Granular characteristics, pollutant removal and algal-bacterial structure 高温发酵液和机械脱水液对藻菌颗粒污泥的影响：颗粒特性、污染物去除和藻菌结构

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-03-01 Epub Date: 2025-12-15 DOI: 10.1016/j.bej.2025.110028

Ao Wang , Peng-fei Yu , Ding Wang , Hai-wen Sun , Bo-mei Hu , Xing-guan Ma , Shi-gang Tu

{"title":"Effects of high-temperature fermentation liquid and mechanically dewatering liquid on algal-bacterial granular sludge: Granular characteristics, pollutant removal and algal-bacterial structure","authors":"Ao Wang , Peng-fei Yu , Ding Wang , Hai-wen Sun , Bo-mei Hu , Xing-guan Ma , Shi-gang Tu","doi":"10.1016/j.bej.2025.110028","DOIUrl":"10.1016/j.bej.2025.110028","url":null,"abstract":"<div><div>Lowering water content and pollutant concentration in sludge is key for sludge reduction and resource reuse. However, the waste liquid generated during this process is highly concentrated, imposing additional environmental burdens. To address this issue, an Algae-bacteria granular sludge (ABGS) system was constructed. This study compared and analyzed pollutant removal performance and microbial interactions within the ABGS when treating both sludge high-temperature fermentation Liquid (HFL) and mechanical dewatering filtrate (MDF). The results showed: The ABGS achieved high removal efficiencies for Total Organic Carbon (950 and 800 mg/L), Total Nitrogen (230 and 130 mg/L), and Phosphorus (65 and 3 mg/L) in both the HFL and the MDF, with rates reaching 93.35 %, 98.07 %, 96.35 % for HFL and 98.18 %, 86.12 %, 89.81 % for MDF, respectively. Both actual wastewater enhanced extracellular polymeric substances (295.56, 315.81 mg/g VSS), integrity coefficient, granular size, and MLVSS/MLSS ratio (than 0.75), improved granular strength of the ABGS.A high-concentration environment of carbon, nitrogen, and phosphorus may activate algal-bacterial cometabolic networks. Microalgae release dissolved oxygen via photosynthesis, enhancing the activity of nitrifying bacteria (<em>Pararhodobacter</em>). Functional complementarity occurs between polyphosphate accumulating organisms such as <em>Paracoccaceae</em> and denitrifiers like <em>Thauera</em>. Through quorum sensing, key metabolic genes (pst, pts, nir, nor) are regulated, establishing a coupled system integrating photosynthetic oxygen supply with simultaneous nitrogen and phosphorus removal.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"227 ","pages":"Article 110028"},"PeriodicalIF":3.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial synthesis of L-fucose from glycerol and glucose in engineered Escherichia coli 用甘油和葡萄糖在工程大肠杆菌中合成L-聚焦物

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-03-01 Epub Date: 2025-12-13 DOI: 10.1016/j.bej.2025.110044

Yongwei Zhang , Jun Yang , Ying Lin , Qi Wang , Shuli Liang

{"title":"Microbial synthesis of L-fucose from glycerol and glucose in engineered Escherichia coli","authors":"Yongwei Zhang , Jun Yang , Ying Lin , Qi Wang , Shuli Liang","doi":"10.1016/j.bej.2025.110044","DOIUrl":"10.1016/j.bej.2025.110044","url":null,"abstract":"<div><div><span>L</span>-Fucose is attracting more attention currently due to its multiple physiological and pharmaceutical functions via fucosylation forms in human body. However, studies regarding the biosynthesis of <span>L</span>-fucose were raised in just recent years, meanwhile the fine rewire of carbon flux in the microbial cell factory is the key for de novo biosynthesis and needs to be further investigated. Herein, we constructed a recombinant strain FZWP03 derived from <em>Escherichia coli</em> MG1655 for production of <span>L</span>-fucose through a combined strategy of genomic integration and plasmid-based expression of pathway genes, in addition to knockout of competing pathway. FZWP03 synthesized 2.68 and 0.96 g/L of <span>L</span>-fucose by shake-flask cultivation from glycerol and glucose, respectively. Then, a <em>pfkA</em> turn-off dynamic switch combining growth phase-dependent promoters (GPPs) and degrons was applied to dynamically fine-tune the carbon flux from fructose-6-phosphate (F6P) to GDP-<span>L</span>-fucose or EMP pathway, and the resulting strain FZWP06 exhibited a significant increase in <span>L</span>-fucose production. Furthermore, the native cryptic plasmids from <em>E</em>. <em>coli</em> Nissle 1917 were applied to construct FZWP08, enabling plasmid stabilization without antibiotic addition in fermentation processes. Finally, with glycerol or glucose as the de novo synthetic substrates, FZWP03, FZWP06, and FZWP08 achieved 21.38, 5.05, and 5.95 g/L <span>L</span>-fucose in fed-batch fermentation, respectively. The technical approaches raised in this study could be applied in the biosynthesis of other compounds.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"227 ","pages":"Article 110044"},"PeriodicalIF":3.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of hydrogels made of a novel recombinant spider silk proteins MaNC and evaluation for drug carriers 一种新型重组蛛丝蛋白MaNC水凝胶的表征及药物载体的评价

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-03-01 Epub Date: 2025-12-06 DOI: 10.1016/j.bej.2025.110040

Kangkang Wang , Guogeng Wang , Zhenzhou Zhao , Xujie Zhou , Hengrui Zhang , Na Li , Hongdan Bao

{"title":"Characterization of hydrogels made of a novel recombinant spider silk proteins MaNC and evaluation for drug carriers","authors":"Kangkang Wang , Guogeng Wang , Zhenzhou Zhao , Xujie Zhou , Hengrui Zhang , Na Li , Hongdan Bao","doi":"10.1016/j.bej.2025.110040","DOIUrl":"10.1016/j.bej.2025.110040","url":null,"abstract":"<div><div>Owing to their good biocompatibility and low immunogenicity, spider silk proteins (spidroins) are promising biopolymers with high potential for various medical applications. Of these material types, spidroin-based hydrogels have attracted significant attention in biomaterials research owing to their non-toxic and excellent mechanical properties. However, reported spidroin hydrogels require extended gelation times. In this study, we engineered a novel recombinant spidroin (MaNC) by fusing the highly soluble N-terminal domain and the self-assembling C-terminal domain derived from major ampullate spidroin. The N-terminal domain confers high solubility on MaNC, while the C-terminal domain provides self-assembly properties, enabling rapid gelation of MaNC. We demonstrated that it spontaneously self-assembles into hydrogels at 37 °C within 10 min, accompanied by the formation of β-sheet structure, without requiring any external additives. It was shown that the self-assembling MaNC hydrogels exhibit no swelling, can be readily loaded with doxorubicin under aqueous conditions, release drug over one month, and that the diffusion rate can be tuned by changing the protein concentration and pH value. Collectively, these data support the notion that our silk hydrogels are fast-gelling, highly adaptable, and cytocompatible materials that self-assemble under mild conditions and are well suited for chemotherapy delivery.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"227 ","pages":"Article 110040"},"PeriodicalIF":3.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Iron-carbon enhanced CW-MFC for treating high COD/TN rural greywater: Performance and microbial synergy in bioenergy recovery 铁碳强化CW-MFC处理高COD/TN乡村灰水：生物能源回收中的性能和微生物协同作用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-03-01 Epub Date: 2025-12-22 DOI: 10.1016/j.bej.2025.110054

Zhiwei Zhu , Yahui Li , Junyong He , Yulian Li , Peidong Hong , Chao Xie , Zijian Wu , Jiandong Lu , Dandan Yang , Lingtao Kong

{"title":"Iron-carbon enhanced CW-MFC for treating high COD/TN rural greywater: Performance and microbial synergy in bioenergy recovery","authors":"Zhiwei Zhu , Yahui Li , Junyong He , Yulian Li , Peidong Hong , Chao Xie , Zijian Wu , Jiandong Lu , Dandan Yang , Lingtao Kong","doi":"10.1016/j.bej.2025.110054","DOIUrl":"10.1016/j.bej.2025.110054","url":null,"abstract":"<div><div>This study developed an iron-carbon enhanced constructed wetland-microbial fuel cell (ICCW-MFC) to treat real rural greywater with high COD/TN ratios (13.1–16.0). The ICCW-MFC significantly improved pollutant removal, especially under extended hydraulic retention time (HRT), increasing removal efficiencies by 19.4 % (COD), 16.5 % (TN), 20.7 % (NH₄⁺-N), and 13.6 % (TP). The ICCW-MFC consistently produced higher quality effluent, with average reductions of 5.1 % (COD), 4.5 % (TN), 7.3 % (NH₄⁺-N), and 5.5 % (TP) compared to the control. Bioenergy recovery was substantially boosted, with a 2.8-fold higher current density and an 8-fold greater maximum power density compared to a conventional CW-MFC (lab control). Microbial diversity increased significantly (P < 0.001) at the anode, enriching electroactive genera (e.g., <em>Geobacter</em>) and forming a synergistic degradation network. PCA identified HRT as the dominant operational factor, while 3D-EEM confirmed effective degradation of tryptophan-like organics. The ICCW-M demonstrates promise for decentralized greywater treatment with concurrent energy recovery.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"227 ","pages":"Article 110054"},"PeriodicalIF":3.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced microalgae cultivation and simultaneous achievement of nutrient removal from actual anaerobic digestion effluent of a full-scale kitchen waste plant 增强微藻培养，同时实现从实际厌氧消化污水中去除营养物

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-03-01 Epub Date: 2025-12-08 DOI: 10.1016/j.bej.2025.110043

Wenwei Zhang , Yi Zhang , Meixing Xiao , Sijin Wu , Jialu Liu , Lie Yang

{"title":"Enhanced microalgae cultivation and simultaneous achievement of nutrient removal from actual anaerobic digestion effluent of a full-scale kitchen waste plant","authors":"Wenwei Zhang , Yi Zhang , Meixing Xiao , Sijin Wu , Jialu Liu , Lie Yang","doi":"10.1016/j.bej.2025.110043","DOIUrl":"10.1016/j.bej.2025.110043","url":null,"abstract":"<div><div>Conventional microalgae cultivation media are cost-prohibitive, necessitating alternative nutrient sources to enable economically viable biomass production for value-added applications. This study investigated the cultivation of <em>Chlorella</em> and <em>Scenedesmus</em> using actual anaerobic digestion effluent (ADE) from untreated kitchen waste. Despite an unfavorable C:N:P ratio, indigenous bacteria and other potential stressors, ADE culture conditions promoted superior growth rates and biomass accumulation in <em>Chlorella</em>. Aeration further increased biomass accumulation, with <em>Chlorella</em> achieving significantly higher yields than <em>Scenedesmus</em>. Specifically, <em>Chlorella</em> attained a biomass yield of 0.537 g/L and a growth rate of 0.218 μ/d. This enhanced performance is attributed to <em>Chlorella</em>'s greater ammonia tolerance and efficient Ca²⁺ uptake (≤99.21 %). Remarkably, aeration increased <em>Chlorella</em>'s extracellular polymeric substance (EPS) content by 88.56 %. And this system achieved simultaneous nutrient removal (37 % COD, 87 % TP) and biomass production, with potential for enhanced pollutant removal efficiency at reduced hydraulic retention times (HRT = 4–6 days). Fluorescence analysis revealed dissolved organic matter (DOM) supported microalgae growth through dual mechanisms: providing a carbon supply and facilitating heavy metal detoxification. These findings indicated that optimizing the pretreatment process of anaerobic digestate to achieve the transformation of complex organic matter in it may further enhance its potential for deep treatment of anaerobic digestate and microalgae culture.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"227 ","pages":"Article 110043"},"PeriodicalIF":3.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0