Biochemical Engineering Journal最新文献_第9页

Machine learning-based predictive modeling and optimization: Artificial neural network-genetic algorithm vs. response surface methodology for black soldier fly (Hermetia illucens) farm waste fermentation 基于机器学习的预测建模与优化：人工神经网络、遗传算法与黑兵蝇（Hermetia illucens）农场废弃物发酵响应面方法

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-17 DOI: 10.1016/j.bej.2025.109685

Oseweuba Valentine Okoro , D.E. Caevel Hippolyte , Lei Nie , Keikhosro Karimi , Joeri F.M. Denayer , Armin Shavandi

{"title":"Machine learning-based predictive modeling and optimization: Artificial neural network-genetic algorithm vs. response surface methodology for black soldier fly (Hermetia illucens) farm waste fermentation","authors":"Oseweuba Valentine Okoro , D.E. Caevel Hippolyte , Lei Nie , Keikhosro Karimi , Joeri F.M. Denayer , Armin Shavandi","doi":"10.1016/j.bej.2025.109685","DOIUrl":"10.1016/j.bej.2025.109685","url":null,"abstract":"<div><div>Recognizing the complexity of non-linear and interdependent biological processes, this study compared the predictive performance of artificial neural network (ANN) models with response surface methodology regression based (RB) models. The research focused on the biological transformation of black soldier fly (<em>Hermetia illucens</em>) farm waste into chitin, facilitated by <em>Lactobacillus paracasei</em>. Key parameters of time (1–7 days), temperature (30–40 °C), substrate concentration (7.5–20 wt%), and inoculum concentration (5–15 v/v%), were evaluated for their impact on demineralization and deproteinization subprocesses and subsequently optimized. It was determined that the ANN models outperformed RB models, with R² values of 0.950 and 0.959 for DP% and DD%, compared to 0.677 and 0.720 for RB models. While both models, optimized using a multi-objective genetic algorithm (MOGA) and a desirability function respectively, produced comparable optimal results, differences emerged in process variable analysis. Main effects plots (RB) and one way partial dependence plots (ANN) revealed conflicting parameter influences, highlighting the limitations of regression models in complex systems. This study highlights the superiority of ANN-MOGA in addressing biological complexity and recommends its use especially if RB models show suboptimal predictive capabilities.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"218 ","pages":"Article 109685"},"PeriodicalIF":3.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143508783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the antibiotics removal ability of Monoraphidium contortum 揭示扭曲单胞菌对抗生素的去除能力

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-17 DOI: 10.1016/j.bej.2025.109686

Larissa T.A. Costa-Ramos , Marcus V.X. Senra , Guilherme H.S. Nogueira , Ralf R. Ramalho-Júnior , Sandro J. Andrade , Evellin E. Santo , Aline K. Gohara-Beirigo , Juliana Gomes Santos , Livia S. Ferreira-Camargo , João Carlos M. Carvalho , Marcelo C. Matsudo

{"title":"Unveiling the antibiotics removal ability of Monoraphidium contortum","authors":"Larissa T.A. Costa-Ramos , Marcus V.X. Senra , Guilherme H.S. Nogueira , Ralf R. Ramalho-Júnior , Sandro J. Andrade , Evellin E. Santo , Aline K. Gohara-Beirigo , Juliana Gomes Santos , Livia S. Ferreira-Camargo , João Carlos M. Carvalho , Marcelo C. Matsudo","doi":"10.1016/j.bej.2025.109686","DOIUrl":"10.1016/j.bej.2025.109686","url":null,"abstract":"<div><div>This study shows the <em>Monoraphidium contortum</em> potential for removing antibiotics, specifically sulfamethoxazole (SMX) and trimethoprim (TMP), from wastewater. Experiments were conducted using a bench-scale tubular photobioreactor, where the microalga demonstrated the ability to reduce antibiotic concentrations while simultaneously removing inorganic nutrients and producing commercially valuable biomass. When each antibiotic was individually added in the culture medium, <em>M. contortum</em> was able to remove 42.3 % of SMX and 28.6 % of TMP. However, when both antibiotics were introduced simultaneously, SMX removal decreased to 7 %. Additionally, the biochemical composition of the resulting biomass revealed stable lipid content, with a fatty acid profile suitable for biodiesel production. By genome analysis, it was possible to identify a multicopper oxidase enzyme, with potential antibiotic-degrading properties, shedding light on the mechanisms behind the removal process. These findings suggest that <em>M. contortum</em> could play a crucial role in bioremediation and sustainable wastewater treatment, generating feedstock for bioenergy production.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109686"},"PeriodicalIF":3.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable intensified conversion of waste corn cob to 5-HMF by synergistic action between infrared radiation and cellulase grafted nano-titania-silica bio-photocatalyst 红外辐射与纤维素酶接枝纳米二氧化钛生物光催化剂协同作用下废玉米芯持续强化转化为5-羟甲基糠醛

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-16 DOI: 10.1016/j.bej.2025.109675

Sohini Roy Choudhury, Rajat Chakraborty

{"title":"Sustainable intensified conversion of waste corn cob to 5-HMF by synergistic action between infrared radiation and cellulase grafted nano-titania-silica bio-photocatalyst","authors":"Sohini Roy Choudhury, Rajat Chakraborty","doi":"10.1016/j.bej.2025.109675","DOIUrl":"10.1016/j.bej.2025.109675","url":null,"abstract":"<div><div>This study reports the pioneering investigation into the synergistic effects of nano-silica-titania immobilized cellulase bio-photocatalyst and visible near-infrared radiation (VIS-NIR) on the intensification of 5-hydroxymethylfurfural (5-HMF) synthesis from delignified corn cob (DCC). A nano-bio-photocatalyst, silica/titania-cellulase (ST-C),was engineered where nano-titania-silica core-shell spheres were synthesized and conjugated with <em>Aspergillus niger</em> derived cellulase enzyme. With a band gap of 2.83 eV, the optimized ST-C exhibited its potential for enhanced bio-photocatalytic efficacy in 5-HMF synthesis via bio-photochemical conversion (BPC) of DCC in a quartz iodine irradiated self-vibrating batch bioreactor (QIISBR) (100 W,visible range:380–700 nm).The synergistic effect between VIS-NIR of QIISBR and the prepared ST-C bio-photocatalyst was evident from the enhanced 5-HMF yield(74.24 mol%) compared to dark run (69.3 mol%) at the optimal 60°C BPC temperature, 1 wt% bio-photocatalyst concentration, 60 min reaction time, 30 w/w Biomass-to-Water ratio as determined by Taguchi Structured Orthogonal Design (TSOD). The VIS-NIR system also offered 68.5 % energy savings compared to dark run. Remarkably,the immobilized enzyme preserved 93 % of its initial activity after nine consecutive uses, showcasing its high operational stability and recyclability attributes. The Life Cycle Sustainability Evaluation (LCSE) revealed reduction in environmental burdens, marked by 69.23 % decrease in Carbon Footprint,45.8 % decline in Human Toxicity Potential,50.1 % reduction in Water Consumption Potential compared to conventional process.The developed process demonstrating synergistic application of VIS-NIR and bio-photocatalyst could procreate a greener pathway through sustainable valorization of lignocellulosic biomass towards synthesis of 5-HMF and similar platform chemicals.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109675"},"PeriodicalIF":3.7,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Degradation of β-HCH by Enterobacter sp. CS01: Characteristics, mechanism and application in soil remediation 肠杆菌 CS01 对 β-HCH 的降解：特性、机理及在土壤修复中的应用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-15 DOI: 10.1016/j.bej.2025.109673

Huijun Shi , Qing Chen , Yanpeng Liang , Litang Qin , Honghu Zeng , Xiaohong Song

{"title":"Degradation of β-HCH by Enterobacter sp. CS01: Characteristics, mechanism and application in soil remediation","authors":"Huijun Shi , Qing Chen , Yanpeng Liang , Litang Qin , Honghu Zeng , Xiaohong Song","doi":"10.1016/j.bej.2025.109673","DOIUrl":"10.1016/j.bej.2025.109673","url":null,"abstract":"<div><div>β-Hexachlorocyclohexane (β-HCH) is a persistent organochlorine pesticide that poses a significant threat to the ecological environment, necessitating the urgent development of effective degradation methods. Microbial degradation has demonstrated substantial potential among various bioremediation techniques due to its environmentally friendly and economical characteristics. This study evaluates the degradation capability of <em>Enterobacter</em> sp. CS01 on β-HCH, its physiological responses, and its potential application in soil remediation. Under optimal conditions (pH 7, 30°C), 51 % of β-HCH was effectively removed. Metabolomics and antioxidant enzyme activity analyses revealed that CS01 defends against oxidative damage by modulating the activities of superoxide dismutase (SOD) and catalase (CAT), involving butyrate, alanine, aspartate, and glutamate metabolism, as well as the pentose phosphate pathway. CS01 converts β-HCH into less toxic intermediates through dichloride elimination, dehalogenation of hydrogen, and hydrolysis reactions. Soil experiments indicated that soil enzyme activities (S-POD, S-DHA, S-PPO) are closely related to the degradation of β-HCH, with the order of carbon source utilization being esters, amino acids, and sugars. This study provides new insights into the microbial degradation mechanisms of organochlorine pesticides and aids in the development of more efficient and environmentally friendly degradation technologies.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109673"},"PeriodicalIF":3.7,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of electric field on microbial metabolism in petroleum-polluted soil: Insights from microbial function and carbon utilization characteristics 电场对石油污染土壤中微生物代谢的影响：从微生物功能和碳利用特征看问题

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-13 DOI: 10.1016/j.bej.2025.109665

Ruijuan Fan , Mengke Zhu , Bin Yang , Xingfu Yan

{"title":"Effects of electric field on microbial metabolism in petroleum-polluted soil: Insights from microbial function and carbon utilization characteristics","authors":"Ruijuan Fan , Mengke Zhu , Bin Yang , Xingfu Yan","doi":"10.1016/j.bej.2025.109665","DOIUrl":"10.1016/j.bej.2025.109665","url":null,"abstract":"<div><div>This study investigated how the electric field enhances microbial metabolism during bio-electrokinetic (BIO-EK) remediation and identified factors hindering sustainable pollutant degradation. The results showed that the degradation rate of total organic carbon (TOC) was higher in BIO-EK than in bioremediation and electrokinetic processes, by up to 2.22 and 1.54 times, respectively. Similarly, the degradation efficiency of total petroleum hydrocarbons (TPH) in BIO-EK was 1.58 and 1.81 times higher than in the bioremediation and electrokinetic groups. In BIO-EK, microbial biomass carbon (MBC) content was up to 6.13 times higher than in the bioremediation group, and dissolved organic carbon (DOC) content was 2.30 and 1.26 times higher than in the bioremediation and electrokinetic groups, respectively. This indicates that the electric field promoted the conversion of TOC to DOC, which was assimilated by microorganisms to generate MBC. The analysis of the structure and functional genes of soil microbial communities revealed that the electric field accelerated the degradation of key petroleum hydrocarbon components and the soil carbon cycle. However, degradation rates varied, and MBC and DOC levels declined in later stages, indicating discontinuous pollutant degradation. The analysis of the ratio of organic carbon derived from petroleum and soil sources (<sup>12</sup>Cp/<sup>12</sup>Cs) revealed intermittent use of petroleum-derived carbon, potentially limiting the sustainable degradation of pollutants. The research results provide insights for improving BIO-EK remediation efficiency.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109665"},"PeriodicalIF":3.7,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A model based approach for monitoring Bordetella pertussis fermentation with an inline spectro-fluorescence probe 基于模型的在线荧光光谱探针监测百日咳杆菌发酵的方法

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-13 DOI: 10.1016/j.bej.2025.109674

Abhishek Mishra , Michael Vitelli , Boris Tartakovsky , Ibrahim M. Tamer , Hector Budman

{"title":"A model based approach for monitoring Bordetella pertussis fermentation with an inline spectro-fluorescence probe","authors":"Abhishek Mishra , Michael Vitelli , Boris Tartakovsky , Ibrahim M. Tamer , Hector Budman","doi":"10.1016/j.bej.2025.109674","DOIUrl":"10.1016/j.bej.2025.109674","url":null,"abstract":"<div><div>Presently, the extraction of the antigen pertactin poses a challenge in the manufacturing of the whooping cough vaccine due to its low and variable yield [1]. In this work, a hybrid model that combines empirical and mechanistic parts and in-line fluorescence measurements is used to design an estimator for monitoring the manufacturing process in bioreactors. The empirical part of the hybrid model uses Partial Least Squares (PLS) regression to estimate biomass, carbon source, and pertactin productivity from fluorescence data. In view that significant correlations are observed between oxidative stress and productivity, the mechanistic part of the hybrid model is based on key oxidative reaction pathways. Estimation based on a hybrid model is shown to improve the prediction accuracy of antigen productivity as compared to purely empirical or purely mechanistic model-based estimators. The proposed estimator enables real-time monitoring of the manufacturing process and opens the possibility of future implementation of mid-point corrective actions.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109674"},"PeriodicalIF":3.7,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing bamboo biomass for sustainable isobutanol production using genetically engineered Escherichia coli 利用转基因大肠杆菌优化竹子生物量以实现可持续的异丁醇生产

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-11 DOI: 10.1016/j.bej.2025.109669

Said Nawab , Zahoor , Syed Bilal Shah , Mujeeb Ur Rahman , Hareef Ahmed Keerio , Inamur Rahman

{"title":"Optimizing bamboo biomass for sustainable isobutanol production using genetically engineered Escherichia coli","authors":"Said Nawab , Zahoor , Syed Bilal Shah , Mujeeb Ur Rahman , Hareef Ahmed Keerio , Inamur Rahman","doi":"10.1016/j.bej.2025.109669","DOIUrl":"10.1016/j.bej.2025.109669","url":null,"abstract":"<div><div>Researchers are finding sustainable fuel alternatives due to the growing costs and environmental issues related to fossil energy. Isobutanol is an attractive biofuel with a higher energy content than ethanol and the potential to replace gasoline. Bamboo emerges as a promising and cost-effective feedstock for isobutanol production due to its fast growth and high holocellulosic content. By using response surface methodology, this study optimized glucose recovery from bamboo biomass by varying pretreatment temperature (30–70°C), reaction time (0.5–6 h), and NaOH concentration (0.5–3 %). The maximum glucose recovery was achieved using 1 % NaOH at 68°C for 6 hours, resulting in improved cellulose and reduced hemicellulose and lignin content in bamboo. This process released 31.01 mg/mL glucose, representing 65 % of available sugars. Structural modifications of the untreated and alkali-treated bamboo biomass were confirmed through Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Isobutanol fermentation with the engineered <em>E. coli</em> strain JCL260 produced 2.28 g/L of isobutanol from enzymatic hydrolysate, achieving 22.86 % of the theoretical maximum. This study determines that cellulose from bamboo has been efficiently transformed into isobutanol through fermentation, although at a lower concentration associated with pure glucose.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109669"},"PeriodicalIF":3.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and study of CDs-WO3 composites with enhanced photocatalytic antimicrobial properties and degradation of dyes 具有增强光催化抗菌和降解染料性能的CDs-WO3复合材料的制备与研究

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-11 DOI: 10.1016/j.bej.2025.109670

Qing Wang , Guanbin Wen , Zhengfang Yang , Qianqian Guo , Bin Zhang , Yanli Nie , Dongquan Wang

{"title":"Preparation and study of CDs-WO3 composites with enhanced photocatalytic antimicrobial properties and degradation of dyes","authors":"Qing Wang , Guanbin Wen , Zhengfang Yang , Qianqian Guo , Bin Zhang , Yanli Nie , Dongquan Wang","doi":"10.1016/j.bej.2025.109670","DOIUrl":"10.1016/j.bej.2025.109670","url":null,"abstract":"<div><div>In this study, carbon dots (CDs) and tungsten trioxide (WO<sub>3</sub>) were combined to prepare CDs-WO<sub>3</sub> composites for photocatalytic treatment of bacteria and organic dyes in wastewater. The optical properties, antimicrobial properties, antimicrobial mechanism, and photocatalytic degradation performance were also investigated. The CDs-WO<sub>3</sub> composites exhibited enhanced UV absorption intensity, broadened visible light absorption range, good charge transfer and carrier separation abilities, and reduced bandgap. The antimicrobial rate of CDs-WO<sub>3</sub> against <em>Staphylococcus aureus</em> can reach 98 %. The antibacterial mechanism of CDs-WO<sub>3</sub> was found to be the reactive oxygen species damage mechanism. Electron paramagnetic resonance (EPR) analysis revealed that the reactive oxygen species signal intensity of CDs-WO<sub>3</sub> was significantly higher than that of WO<sub>3</sub>, indicating that more <strong>·</strong>O<sub>2</sub><sup><strong>-</strong></sup> and <strong>·</strong>OH were produced. The analysis of bacterial cell activity demonstrated that CDs-WO<sub>3</sub> composites reduced the activity of respiratory chain dehydrogenase in bacteria, which resulted in increased lipid peroxidation in the cell membrane. The photocatalytic degradation performance showed that the photocatalytic degradation efficiency of CDs-WO<sub>3</sub> for methylene blue and malachite green reached 87 % and 88.04 %, respectively. The application of CDs-WO<sub>3</sub> in real water bodies also showed good antibacterial effect and dye degradation.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109670"},"PeriodicalIF":3.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research on the removal of fluoride from low-concentration fluorine-containing industrial wastewater using adsorption methods 吸附法去除低浓度含氟工业废水中的氟的研究

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-10 DOI: 10.1016/j.bej.2025.109668

Benfu Luo , Yuhang Liu , Yujing Yan , Haixing He , Jie Yu , Qiang Chen

{"title":"Research on the removal of fluoride from low-concentration fluorine-containing industrial wastewater using adsorption methods","authors":"Benfu Luo , Yuhang Liu , Yujing Yan , Haixing He , Jie Yu , Qiang Chen","doi":"10.1016/j.bej.2025.109668","DOIUrl":"10.1016/j.bej.2025.109668","url":null,"abstract":"<div><div>To address the need for deep fluoride removal of low-concentration fluorine-containing industrial wastewater (2–10 mg/L) to below 1.5 mg/L in centralized industrial wastewater treatment plants, a study was conducted using adsorption methods. The adsorption performance and influencing factors of four adsorbent materials—active alumina (AA), hydroxyapatite (HAP), fly ash (FA), and bone char (BC)—were investigated. The results indicate that positioning the adsorption fluoride removal filter at the tail end of the industrial wastewater treatment process yields a fluoride removal efficiency that is over 10 % higher compared to placing it at the front end. The investigation into the adsorption effectiveness of the four adsorbents revealed that HAP dosage only needs 2 g to reduce 250 ml fluorinated industrial wastewater containing 2–10 mg/L to the target concentration of 1.5 mg/L or less. Moreover, the adsorption process requires only 30 minutes. From the perspective of adsorption performance, HAP is the most effective, followed by AA. However, considering the cost of these adsorbents, AA is more suitable as an adsorbent for treating low-concentration fluorine-containing wastewater. The findings of this study can serve as a valuable reference for the process design of deep adsorption fluoride removal in projects involving fluorine-containing industrial wastewater.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109668"},"PeriodicalIF":3.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular modification and application of the key enzyme CYP109E1-H in 25-hydroxyvitamin D3 biosynthesis 25-羟基维生素D3生物合成关键酶CYP109E1-H的分子修饰及应用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-10 DOI: 10.1016/j.bej.2025.109667

Fan He , Yulin He , Jingyi Zhou , Junheng Gou , Qiaoqiao Ma , Xiaomei Zhang , Jinsong Shi , Zhenghong Xu , Hui Li

{"title":"Molecular modification and application of the key enzyme CYP109E1-H in 25-hydroxyvitamin D3 biosynthesis","authors":"Fan He , Yulin He , Jingyi Zhou , Junheng Gou , Qiaoqiao Ma , Xiaomei Zhang , Jinsong Shi , Zhenghong Xu , Hui Li","doi":"10.1016/j.bej.2025.109667","DOIUrl":"10.1016/j.bej.2025.109667","url":null,"abstract":"<div><div>25-Hydroxyvitamin D<sub>3</sub> (25-OH-VD<sub>3</sub>) is one of the primary active forms of vitamin D<sub>3</sub> (VD<sub>3</sub>) in the human body and has significant medicinal value. The traditional organic synthesis method of 25-OH-VD<sub>3</sub> is very complex and the yield is very low. Biocatalysis can simplify the synthesis steps, with relatively mild reaction conditions and easy access to raw materials. Therefore, the production of 25-OH-VD<sub>3</sub> through microorganisms has been considered a promising alternative to chemical synthesis. However, the recombinant expression system demonstrated poor catalytic activity and low substrate conversion efficiency, hindering the further application of 25-OH-VD<sub>3</sub> biological synthesis. The purpose of this study is to improve the efficiency of biotransformation and increase the production of 25-OH-VD<sub>3</sub> by modifying VD<sub>3</sub> hydroxylase. Initially, the positive mutant CYP109E1<sup>I85F</sup>-H was obtained through saturation mutation. The concentration of 25-OH-VD<sub>3</sub> was increased to 1.57 mg/L, which was 1.9 times that of the wild-type (WT) strain. Then, the electron transfer engineered strain WB600-pMA5-CYP109E1<sup>I85F</sup>-H-Fdx-FdR was constructed to further improve the efficiency of electron transfer, with a 14.5 % increase in product concentration. Finally, 14.53 mg/L of 25-OH-VD<sub>3</sub> was achieved in optimized SR medium supplemented with dimethyl sulfoxide (DMSO) as co-solvent. The combination of semi-rational molecular modification strategy and optimization of the catalytic system increased the production of 25-OH-VD<sub>3</sub> by 16.9 times compared with the WT strain, providing guidance for CYP109E1-H microbial synthesis of 25-OH-VD<sub>3</sub>.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"218 ","pages":"Article 109667"},"PeriodicalIF":3.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0