Biochemical Engineering Journal最新文献_第9页

Immobilization of Bgps-Expressing Escherichia coli cells coated with ZIF-67 for the production of rare ginsenoside F1 用 ZIF-67 涂布固定表达 Bgps 的大肠杆菌细胞以生产稀有人参皂苷 F1

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-14 DOI: 10.1016/j.bej.2024.109432

引用次数: 0

Acetic acid stress and utilization synergistically enhance squalene biosynthesis in Candida glycerinogenes 乙酸胁迫和利用协同促进甘油酸根念珠菌角鲨烯的生物合成

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-10 DOI: 10.1016/j.bej.2024.109413

{"title":"Acetic acid stress and utilization synergistically enhance squalene biosynthesis in Candida glycerinogenes","authors":"","doi":"10.1016/j.bej.2024.109413","DOIUrl":"10.1016/j.bej.2024.109413","url":null,"abstract":"<div><p>Squalene is widely used in industries such as vaccines and cosmetics. The traditional carbon source for microbial synthesis of squalene is glucose, but its long metabolic pathway leads to inefficient synthesis, limiting industrial production. This study uses acetate as a carbon source, taking advantage of its stress effects and the ability to shorten metabolic pathways to enhance the synthesis of squalene. To promote the accumulation of squalene, the MVA pathway and NADPH supply pathways were enhanced. To alleviate acetic acid stress, acetic acid conversion was accelerated by overexpressing <em>ACS1</em>, and the strain’s tolerance to acetic acid was improved by overexpressing <em>HOG1</em>. Subsequently, the strain was subjected to fed-batch fermentation under acetic acid stress with a carbon source of 6 g/L acetic acid, resulting in a squalene titer of 1834.74 mg/L in shake flasks. The culmination of these strategies, applied during a 96 h fed-batch fermentation with acetic acid-mediated pH control in a 5 L bioreactor, resulted in a squalene titer of 8.76 g/L, a content of 411.24 mg/g DCW, and a productivity of 4.28 mg·h<sup>−1</sup>·g<sup>−1</sup> DCW, marking the highest productivity reported to date. This study provides a novel perspective for the green, economically efficient biosynthesis of squalene.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141708549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effective and green biodegradation of polyethylene microplastics by the screening of a strain with its degrading enzymes 通过筛选具有降解酶的菌株实现聚乙烯微塑料的有效和绿色生物降解

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-10 DOI: 10.1016/j.bej.2024.109429

Yaliang Duan , Yanli Yin , Zifu Ni , Jianguang Liu , Haitao Gui , Dapeng Wu , Xingquan Wu , Le Wang

{"title":"The effective and green biodegradation of polyethylene microplastics by the screening of a strain with its degrading enzymes","authors":"Yaliang Duan , Yanli Yin , Zifu Ni , Jianguang Liu , Haitao Gui , Dapeng Wu , Xingquan Wu , Le Wang","doi":"10.1016/j.bej.2024.109429","DOIUrl":"https://doi.org/10.1016/j.bej.2024.109429","url":null,"abstract":"<div><p>The polyethylene microplastics (MPs) degrading fungi named the strain DL-1 were isolated and screened to reduce the pollution of polyethylene MPs. The strain was preliminarily identified as <em>Aspergillus niger</em> by the sequence homology comparison and phylogenetic tree construction. The biodegradation rate of polyethylene MPs by the strain DL-1 was 7.65 ± 0.92 % after 30 days. The grooves and wrinkles appeared on the surface of MPs after the degradation. During the effective degradation process, the strain DL-1 produced the enzymes (plastic degrading enzymes) to biodegrade the polyethylene MPs. It was showed that the plastic degrading enzymes increased under the induction of polyethylene MPs in the medium, indicating that the enzymes played the important role in biodegradation of polyethylene MPs. Moreover, the ability of strain to biodegrade polyethylene MPs and the mechanism of biodegradation were investigated with varied measurements and characteristics. It enriched the way of microbial degradation of polyethylene MPs and provided the environmentally-friendly biodegradation with the green treatment for MPs.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141607363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient heterotrophic nitrification – aerobic denitrification by a novel bacterium Ralstonia pickettii J4: Isolation, identification, and application 新型细菌 Ralstonia pickettii J4 的高效异养硝化-好氧反硝化作用：分离、鉴定和应用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-06 DOI: 10.1016/j.bej.2024.109417

Ping Yang , Tingting Yao , Xinyi Liu , Aimin Zhang , Jian Zhang , Lina Pang

{"title":"Efficient heterotrophic nitrification – aerobic denitrification by a novel bacterium Ralstonia pickettii J4: Isolation, identification, and application","authors":"Ping Yang , Tingting Yao , Xinyi Liu , Aimin Zhang , Jian Zhang , Lina Pang","doi":"10.1016/j.bej.2024.109417","DOIUrl":"https://doi.org/10.1016/j.bej.2024.109417","url":null,"abstract":"<div><p>The strain <em>Ralstonia pickettii</em> J4 was isolated as a novel heterotrophic nitrification - aerobic denitrification (HN-AD) and its potential in removing nitrogen was assessed. Results indicated that the optimal HN-AD efficiencies by strain J4 were 98.99 % for ammonium nitrogen (NH<sub>4</sub><sup>+</sup>-N) and 88.86 % for total nitrogen, when using glucose as the optimal carbon source at 30 °C and under conditions of carbon/nitrogen ratio of 15, pH of 7, shaking speed of 100 rpm, and initial NH<sub>4</sub><sup>+</sup>-N concentration of 50 mg/L. Analysis results of nitrogen balance and functional genes revealed the possible HN-AD pathway of J4: NH<sub>4</sub><sup>+</sup>-N→ hydroxylamine→ nitrite nitrogen→ nitric oxide→ nitrous oxide→ nitrogen gas, without nitrite accumulation. Strain J4 significantly enhanced the nitrogen removal performance of a sequencing biofilm batch reactor by enriching the functional bacterial community involved in nitrogen removal, for instance, <em>Thauera</em>, <em>Hydrogenophaga</em>, and <em>Flavobacterium</em>. These findings are important for providing bioaugmentation sources regarding nitrogen removal of wastewater treatment.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141593029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overcoming the inconsistent cell culture performance caused by single-use bioreactors during scaling-up the manufacturing process for a therapeutic bispecific antibody 克服一次性生物反应器在扩大治疗性双特异性抗体生产过程中造成的细胞培养性能不稳定问题

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-06 DOI: 10.1016/j.bej.2024.109428

Xiaowei Diao , Xiuhua Huang , Guowei Gu , Jiaxin Li , He Li , Jianzhong Hu

{"title":"Overcoming the inconsistent cell culture performance caused by single-use bioreactors during scaling-up the manufacturing process for a therapeutic bispecific antibody","authors":"Xiaowei Diao , Xiuhua Huang , Guowei Gu , Jiaxin Li , He Li , Jianzhong Hu","doi":"10.1016/j.bej.2024.109428","DOIUrl":"https://doi.org/10.1016/j.bej.2024.109428","url":null,"abstract":"<div><p>Single use technology has been widely used in modern biopharmaceutical process development and manufacturing. However, there are concerns about the adverse effects of disposable materials on bioprocess, product quality and patient safety. Recently, we observed slow cell growth, increased lactate accumulation, and decreased production titer during scaling up a bispecific antibody (BsAb) upstream process from 3 L to 500 L bioreactor. To investigate the phenomenon, we conducted medium incubation and leachable spike-in experiments. We found that cell culture medium incubated in CX5–14 bag caused poor cell culture performance, which was correlated with the concentration of bis(2,4-di-tert-butylphenyl)-phosphate (bDtBPP), a breakdown product of the antioxidant Irgafos®168 in polyethylene film. In addition, when the media were spiked with 0.15 μg/ml or a greater concentration of bDtBPP, slow cell growth and reduced BsAb expression level were observed. In contrast, Aegis5–14 film produced bDtBPP at a level of below detection during medium incubation. Thus, the bispecific-expressing CHO cell line restores normal cell culture performance and production titer when Aegis5–14 single-use bioreactor was employed in large-scale manufacturing. Our results reveal the importance of evaluating leachable profiles from disposable materials during process development of bispecifics and other novel therapeutic modalities.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141593028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maximizing the mRNA productivity for in vitro transcription by optimization of fed-batch strategy 通过优化喂料批处理策略最大限度地提高体外转录的 mRNA 生产率

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-04 DOI: 10.1016/j.bej.2024.109412

Letao Guo , Zhikai Liu , Shirong Song , Wang Yao , Mei Yang , Guangwen Chen

{"title":"Maximizing the mRNA productivity for in vitro transcription by optimization of fed-batch strategy","authors":"Letao Guo , Zhikai Liu , Shirong Song , Wang Yao , Mei Yang , Guangwen Chen","doi":"10.1016/j.bej.2024.109412","DOIUrl":"10.1016/j.bej.2024.109412","url":null,"abstract":"<div><p>In vitro transcription (IVT) is the main manufacturing method to produce mRNA vaccines. In this study, a fed-batch strategy was systematically optimized for IVT process with dual goals of achieving a high reaction rate and maximizing the final mRNA yield simultaneously. Initially various experimental conditions were investigated including Mg<sup>2+</sup>, nucleotide triphosphates (NTP), dithiothreitol (DTT), spermidine, as well as the temperature and ionic strength. It was found that the concentrations of Mg<sup>2+</sup> and NTP had a significant impact on IVT process. Subsequently, under the optimized conditions, dividing the IVT reaction into three distinct phases was proposed to enable more efficient transcription. By optimizing the concentrations of Mg<sup>2+</sup> and NTP in two replenishment processes, our fed-batch strategy resulted in the production of 367.8 μg of mRNA with reduced dsRNA byproducts within 180 min. This was achieved under conditions of a final volume of 30 μL, 250 U T7 RNA polymerase (RNAP), and 2 μg DNA template. In conclusion, the IVT process using a fed-batch approach, rather than an excessive one-time NTP input, is an efficient method to improve productivity given a fixed amount of T7 RNAP and DNA template.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141577819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of oxidative stress on antigen productivity in B. Pertussis cultures 氧化应激对百日咳杆菌培养物抗原产量的影响

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-04 DOI: 10.1016/j.bej.2024.109421

Abhishek Mishra , Sarah Steinbach , Ibrahim M. Tamer , Hector Budman

{"title":"Effect of oxidative stress on antigen productivity in B. Pertussis cultures","authors":"Abhishek Mishra , Sarah Steinbach , Ibrahim M. Tamer , Hector Budman","doi":"10.1016/j.bej.2024.109421","DOIUrl":"https://doi.org/10.1016/j.bej.2024.109421","url":null,"abstract":"<div><p>Whooping cough, also known as pertussis, is an infectious respiratory disease caused by <em>Bordetella pertussis</em>, a type of gram-negative, aerobic, pathogenic bacteria with a coccobacillus shape and an outer capsule. The acellular vaccine for this disease contains a combination of pertussis toxin, fimbriae, filamentous hemagglutinin, and pertactin. The main constraint in the manufacturing of the vaccine relates to pertactin productivity due to its low abundance in the fermentation broth. Being the most abundant nutrient in the media, variability in initial glutamate concentration is hypothesized to be a major cause of process variability. This study proposes the combined use of cytometry and chromatography based separation to study the impact of glutamate-induced oxidative stress on the growth and productivity of pertactin antigen. Surface expression of pertactin was observed using fluorescence microscopy and flow cytometry while extracellular concentration was quantified using affinity chromatography. Oxidative stress levels and secretion of NADPH, a crucial reactant in antioxidant reactions, were monitored in flask and bioreactor experiments. The results established clear correlations between oxidative stress with growth and productivity and differentiate between the reduction in productivity to growth and to synthesis rate of pertactin. These findings are industrially relevant for improving productivity and reducing process variability.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1369703X24002080/pdfft?md5=cba8d2110538ab3cde4ab39c73eb39b7&pid=1-s2.0-S1369703X24002080-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141540874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly efficient capture of Escherichia coli using chitosan-lysozyme modified nanofiber membranes: Potential applications in food packaging and water treatment 利用壳聚糖-溶菌酶改性纳米纤维膜高效捕获大肠杆菌：在食品包装和水处理中的潜在应用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-04 DOI: 10.1016/j.bej.2024.109411

Thi Tam An Tran , Edouard Gnoumou , Bing-Lan Liu , Penjit Srinophakun , Chen‑Yaw Chiu , Chi-Yun Wang , Kuei-Hsiang Chen , Yu-Kaung Chang

{"title":"Highly efficient capture of Escherichia coli using chitosan-lysozyme modified nanofiber membranes: Potential applications in food packaging and water treatment","authors":"Thi Tam An Tran , Edouard Gnoumou , Bing-Lan Liu , Penjit Srinophakun , Chen‑Yaw Chiu , Chi-Yun Wang , Kuei-Hsiang Chen , Yu-Kaung Chang","doi":"10.1016/j.bej.2024.109411","DOIUrl":"https://doi.org/10.1016/j.bej.2024.109411","url":null,"abstract":"<div><p>Functionalized nanofiber membranes play a vital role in water treatment by effectively removing pollutants such as heavy metals and dyes. However, microbial contamination remains a significant challenge. This study created a novel antibacterial nanofiber membrane by grafting chitosan (CS) and lysozyme (LYZ) onto a P-COOH weak ion exchange nanofiber membrane. The physical features of the nanofiber membranes were studied, and the immobilization of CS and LYZ onto P-COOH nanofiber membranes was examined to improve Escherichia coli (E. coli) killing. The membrane exhibited impressive antibacterial properties, achieving approximately 100 % efficacy against E. coli. It maintained high effectiveness even after reuse and storage, with 98.71 % efficacy after five cycles of reuse. Cytotoxicity tests confirmed high biocompatibility with a 100 % viability rate. The innovation of this study lies in the grafting of CS and LYZ onto P-COOH weak ion exchange nanofiber membranes, resulting in a highly efficient antibacterial composite. The P-COOH-CS-LYZ membrane exhibits superior antibacterial efficacy and maintains its high effectiveness after repeated reuse and long-term storage, underscoring its durability and practical applicability. Additionally, its high biocompatibility and sustainable design, using natural and non-toxic agents, represent a significant advancement in eco-friendly water treatment technologies. This dual-functional membrane effectively addresses microbial contamination and pollutant removal, offering a comprehensive solution for water treatment.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141593027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Humic acid enhances adsorption effect: Application foundation of high-temperature composting products for remediation of heavy metals pollution 腐殖酸可增强吸附效果：高温堆肥产品在重金属污染修复中的应用基础

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-03 DOI: 10.1016/j.bej.2024.109415

Feng Ma , Tong Zhu , Youzhao Wang , Xu Li , Mingdong Chang , Chaoyue Zhao , Zhipeng Wang , Haoyu Quan

{"title":"Humic acid enhances adsorption effect: Application foundation of high-temperature composting products for remediation of heavy metals pollution","authors":"Feng Ma , Tong Zhu , Youzhao Wang , Xu Li , Mingdong Chang , Chaoyue Zhao , Zhipeng Wang , Haoyu Quan","doi":"10.1016/j.bej.2024.109415","DOIUrl":"https://doi.org/10.1016/j.bej.2024.109415","url":null,"abstract":"<div><p>High-temperature composting products are rich in humic acid, which contains abundant oxygenated functional groups capable of binding to heavy metals, and thus can be considered for remediation of heavy metals pollution. However, the specific adsorption properties of high-temperature composting humic acid (HHA) remain unclear. In this study, humic acid was extracted from composting products using the acid-base extraction method, and the adsorption effect and mechanism were investigated. The experimental results showed that the maximum adsorption capacity of HHA for Cu(II), Zn(II), and Pb(II) was 69.43 mg/g, 58.55 mg/g, and 65.84 mg/g, respectively, representing an average enhancement of 21.80 % compared to conventional composting humic acid. Spectra analysis revealed that the enhanced adsorption mechanism of HHA was mainly due to a 51.63 % increase in hydroxyl and carboxyl groups, which were bound to heavy metals through chelation reactions. Furthermore, kinetics and thermodynamics studies indicated that the adsorption process was an endothermic reaction and predominantly driven by ion exchange. Additionally, the application of high-temperature composting products is economical and feasible due to their production from cheap organic waste. This study aims to demonstrate the superiority of HHA in the adsorption of heavy metals and enhance the comprehension of the adsorption mechanism, which provides an application foundation of composting products for the remediation of heavy metals pollution.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141542638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the efficiency of various methods to load fluoroquinolones into Escherichia coli outer membrane vesicles as a novel antibiotic delivery platform 评估将氟喹诺酮类药物装入大肠杆菌外膜囊泡作为新型抗生素递送平台的各种方法的效率

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-07-02 DOI: 10.1016/j.bej.2024.109418

{"title":"Evaluation of the efficiency of various methods to load fluoroquinolones into Escherichia coli outer membrane vesicles as a novel antibiotic delivery platform","authors":"","doi":"10.1016/j.bej.2024.109418","DOIUrl":"10.1016/j.bej.2024.109418","url":null,"abstract":"<div><p>The development of novel antibacterial agents that are effective against Gram-negative bacteria is limited primarily by transport issues. This class of bacteria maintains a complex cell envelope consisting of two membrane bilayers, preventing the passage of most antibiotics. These drugs must therefore pass through protein channels called porins; however, many antibiotics are too large to pass through porins, and a common mechanism of acquired resistance is down-regulation of porins. To overcome this transport limitation, we have proposed the use of outer membrane vesicles (OMVs), released by Gram-negative bacteria, which deliver cargo to other bacterial cells in a porin-independent manner. In this work, we systematically studied the ability to load fluoroquinolones into purified <em>Escherichia coli</em> OMVs using <em>in vivo</em> and <em>in vitro</em> passive loading methods, and active loading methods such as electroporation and sonication. We observed limited loading of all of the antibiotics using passive loading techniques; sonication and electroporation significantly increased the loading, with electroporation at low voltages (200 and 400 V) resulting in the greatest encapsulation efficiencies. We also demonstrated that imipenem, a carbapenem antibiotic, can be readily loaded into OMVs, and its administration via OMVs increases the effectiveness of the drug against <em>E. coli</em>. Our results demonstrate that small molecule antibiotics can be readily incorporated into OMVs to create novel delivery vehicles to improve antibiotic activity.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141630268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0