Biochemical Engineering Journal最新文献

{"title":"Assessing the environmental footprint of microalgae biofuel production: A comparative analysis of cultivation and harvesting scenarios","authors":"Yue Wang,&nbsp;Hao Wen,&nbsp;Meili Wu,&nbsp;Xu Liu,&nbsp;Hongwei Yin,&nbsp;Wei Qin,&nbsp;Xichen Zheng,&nbsp;Jia He,&nbsp;Kemin Wei,&nbsp;Xiaomin Kong,&nbsp;Shuhui Liang","doi":"10.1016/j.bej.2024.109571","DOIUrl":"10.1016/j.bej.2024.109571","url":null,"abstract":"<div><div>This study investigated the impact of fixed combinations of three flocculants and four buoy-beads under two cultivation systems on the harvesting efficiency, as well as the environmental performance in different scenarios. Results showed that the harvesting efficiency exhibited a tendency of initially increasing and then decreasing with rising concentrations of buoy-beads and flocculants, with an optimal harvesting efficiency of 98.03 %. Life cycle assessment (LCA) compared the environmental performance of five scenarios. Ecotoxicity Soil Chronic (ESC) and Aquatic Eutrophication EP(P) (AEP(P)) were major environmental impacts. The scenario employing re-frying oil emulsion (RFOE) and aluminum sulfate flocculation (R+A) contributed significantly to Human Toxicity Water (HTW) and Aquatic Eutrophication EP(N) (AEP(N)) with normalized values of 0.0137 and 0.0147, respectively. In the assessment of Global Warming Potential (GWP), R+A was responsible for a high amount of Greenhouse Gas (GHG) emissions (2.826 kg CO₂ eq/100 g of dry algal biomass in photobioreactor (PBR) and 2.917 kg CO₂ eq/ 100 g of dry algal biomass in open raceway ponds (ORP)). Notably, sodium alginate microspheres (SAMs) and aluminum sulfate flocculation (S+A) was considered a more environmentally favorable option, 0.773 kg CO₂ eq and 0.864 kg CO₂ eq GHG emissions of PBR and ORP, respectively. Furthermore, the less GHG emissions of PBR than ORP, making it a more effective solution for reducing emissions and mitigating global warming trends.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109571"},"PeriodicalIF":3.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142655024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioenhancement mechanism of PVA-SA immobilized composite strains Alishewanella fetalis and Exiguobacterium profundum in pyridine degradation","authors":"Min Gao ,&nbsp;Yuan Shen ,&nbsp;Yao Peng ,&nbsp;Feiyang Tan ,&nbsp;Yingshun Lv ,&nbsp;Changjie Zhu ,&nbsp;Yaxin Guo ,&nbsp;Xuan Liu","doi":"10.1016/j.bej.2024.109559","DOIUrl":"10.1016/j.bej.2024.109559","url":null,"abstract":"<div><div>Coal chemical wastewater (CCW) represents a type of recalcitrant organic wastewater characterized by its intricate composition and high concentration of pollutants, posing a severe threat to global aquatic environments and public health. This study focuses on the degradation of pyridine, a notoriously persistent organic pollutant in CCW, through the identification and application of two highly efficient pyridine-degrading bacterial strains: <em>Alishewanella fetalis</em> (Al-f3) and <em>Exiguobacterium profundum</em> (Ex-p). These strains were immobilized using a polyvinyl alcohol-sodium alginate (PVA-SA) matrix to investigate their bioaugmentation mechanisms in the pyridine degradation process. The findings indicate that strains Al-f3 and Ex-p achieved degradation rates of 95.94 % and 97.83 %, respectively, for an initial pyridine concentration of 200 mg/L at 96 hours. When strains Al-f3 and Ex-p were mixed in equal proportions and immobilized within PVA/SA beads, a degradation rate of 81.06 % was reached within 48 hours, with the efficiency increasing significantly by 96 hours. This enhancement is attributed primarily to the marked increase in enzymatic activity post-immobilization, achieving 17.13 μmol/mg·min, and the elevated secretion of extracellular proteins and polysaccharides, measured at 3.47 mg/L and 1.03 mg/L respectively within 48 hours. Notably, in the immobilized mixed culture system, the total organic carbon (TOC) was reduced to a mere 0.03 mg/L within 72 hours, with a removal rate of 92.31 %. These outcomes not only demonstrate the bioaugmentation role of the immobilized mixed strains in degrading pyridine but also offer novel solutions for the biodegradation of other organic contaminants in CCW, thereby enhancing the treatment efficiency of such wastewater. Given their large specific surface area and cost-effectiveness, PVA/SA immobilization matrices serve as efficient biocarriers in the biodegradation processes for treating CCW. This research provides innovative strategies and methods for the biotreatment of recalcitrant industrial wastewater.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109559"},"PeriodicalIF":3.7,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel lateral flow assay for lead ion detection based on G-quadruplex","authors":"Linjiao Ren ,&nbsp;Jingtong Sun ,&nbsp;Shilin Ma ,&nbsp;Diankang Wang ,&nbsp;Rubin Qi ,&nbsp;Pei Zhang ,&nbsp;Qingfang Zhang ,&nbsp;Zirui Qin ,&nbsp;Liying Jiang","doi":"10.1016/j.bej.2024.109562","DOIUrl":"10.1016/j.bej.2024.109562","url":null,"abstract":"<div><div>Lead ion residues pose potential health risks to humans. To develop a cost-effective and user-friendly portable lead ion detection method, a novel lateral flow test strip was designed and fabricated based on the G-quadruplex structure. Initially, G4 and its complementary strand antiG4 are in a double-stranded form in the assay solution. After adding lead ions, these ions compete with antiG4 to bind to G4 and form G-quadruplex structures, thus releasing a large number of antiG4 single strands. Through base pairing, one end of the antiG4 was linked to a recognition element containing gold nanoparticles, while the other end was captured by a test line probe, resulting in a red band on the test line. The color change of the test line was positively correlated with the concentration of lead ions. Results showed that by observing the color change of the test line under optimized experimental conditions, lead ion concentration could be detected with a visual detection limit of 20 nM. Quantitative analysis using ImageJ software indicated that the test strip had a linear detection range of 10–2000 nM, with a detection limit of 7.32 nM and significant specificity. The recovery rate in bottled drinking water ranged from 91.19 % to 126.04 %, providing a portable and simple new method for on-site detection of residual lead ions in water environments.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109562"},"PeriodicalIF":3.7,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated strategies for engineering ferulic acid decarboxylase with tunnel conformation and substrate pocket for adapting non-natural substrates","authors":"Xiaoni Zhu,&nbsp;Yanbin Feng,&nbsp;Mingdong Wang,&nbsp;Shang Li,&nbsp;Hongfei Li,&nbsp;Lin Liu,&nbsp;Song Xue","doi":"10.1016/j.bej.2024.109566","DOIUrl":"10.1016/j.bej.2024.109566","url":null,"abstract":"<div><div>Ferulic acid decarboxylase (Fdc1) catalyzes the decarboxylation of ferulic acid derivatives from lignin with broad substrate spectra. However, the catalytic efficiency of Fdc1 across non-natural substrates with bulky substituents attached to the benzene ring limits its application. Here, an integrated strategy was developed to engineer ScFdc1 from <em>Saccharomyces cerevisiae</em>, specifically targeting 4-acetoxycinnamic acid for the production of 4-acetoxystyrene, which was widely used in photoresists monomers. The strategy entailed the integrating two structural aspects of the enzyme, the conformation of the access tunnel and the substrate binding pocket. Six mutants from a 1248-variant library with significant impacts on enzyme performance were identified. Integration of the two aspects of the mutants achieved the ScFdc1_F397V/I398L/T438P/P441V variant, exhibiting over an 11.8-fold activity improvement towards 4-acetoxycinnamic acid. The variant adopts an open tunnel conformation against that the closed state in the wild type, as revealed by structural analysis with the minimal distance at the bottleneck of the tunnel increased from 0.7 to 1.4 nm. Additionally, a reshaped binding pocket is identified which facilitates the substrate accessibility and binding affinity confirmed by enzymatic and molecular dynamics analysis. The results not only provide strategies for enzyme engineering through the accumulation of beneficial mutants, but also offer a promising route for sustainable styrene derivative production.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109566"},"PeriodicalIF":3.7,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functionalized polyacrylonitrile nanofiber membranes with carbonic anhydrase for enhanced carbon dioxide capture and conversion: A performance study","authors":"Shen-Yuan Yang ,&nbsp;Jia-Yih Lin ,&nbsp;Pei-Rong Li ,&nbsp;Nguyen The Duc Hanh ,&nbsp;Penjit Srinophakun ,&nbsp;Bing-Lan Liu ,&nbsp;Chen-Yaw Chiu ,&nbsp;I-Son Ng ,&nbsp;Kuei-Hsiang Chen ,&nbsp;Yu-Kaung Chang","doi":"10.1016/j.bej.2024.109570","DOIUrl":"10.1016/j.bej.2024.109570","url":null,"abstract":"<div><div>As industrial activity rises, atmospheric carbon dioxide levels have significantly contributed to global warming and climate change. Developing effective carbon dioxide capture technologies is imperative to mitigate these effects. Carbonic anhydrase (CA) enzymes represent one of the most promising solutions due to their rapid reaction rates, environmental safety, and efficiency in facilitating CO₂ conversion. This study takes a novel approach by investigating the immobilization of CA on functionalized polyacrylonitrile (PAN) nanofiber membranes to enhance CO₂ conversion and mineralization. PAN nanofibers were fabricated via electrospinning and chemically modified to introduce carboxylic groups, resulting in NM-COOH nanofiber membranes. In addition, amine groups from chitosan (CS) were incorporated to form NM-COOH-CS nanofiber membranes. The immobilization of CA on these membranes revealed that covalent attachment through NM-COOH significantly enhances catalytic activity compared to physical attachment methods. The NM-COOH-CA membrane exhibited superior performance, achieving efficient conversion of CO₂ into HCO₃⁻ and promoting CaCO₃ mineralization. It reached a precipitation efficiency of 11.77 mg CaCO₃ per gram of membrane-active unit (WAU) and maintained 63.12 % of its initial CaCO₃ production over five cycles over five weeks. This study presents a novel, eco-friendly approach to greenhouse gas reduction, emphasizing the effectiveness of CA-immobilized nanofiber membranes. The findings mainly highlight the advantages of carboxylic functional groups in enhancing CA performance, paving the way for future research in carbon capture technologies.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109570"},"PeriodicalIF":3.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic acetyl-CoA augmentation strategy (SATS) for improved terpenoid biosynthesis in Saccharomyces cerevisiae","authors":"Yiying Huo ,&nbsp;Pan Feng ,&nbsp;Haoran Bi ,&nbsp;Kai Wang ,&nbsp;Yang Zhang ,&nbsp;Yunming Fang ,&nbsp;Meng Wang ,&nbsp;Tianwei Tan","doi":"10.1016/j.bej.2024.109572","DOIUrl":"10.1016/j.bej.2024.109572","url":null,"abstract":"<div><div>Terpenoids are widely applied in pharmaceuticals, fragrances and biofuels. Acetyl-CoA, as a crucial precursor in the mevalonate pathway, is stringently regulated intracellularly, thereby limiting the biosynthesis of terpenoids. This study develops a synergistic acetyl-CoA augmentation strategy (SATS) to establish a robust platform for terpenoid biosynthesis in <em>Saccharomyces cerevisiae</em>. Optimizing coenzyme A biosynthesis via global regulation of the pantothenic acid module improved intracellular acetyl-CoA levels by 3.26-fold. Enhancing acetyl phosphate supply was achieved by constructing a PPP-PK-PTA pathway, which improved intracellular acetyl-CoA by 1.92-fold. By combining these techniques, acetyl-CoA levels in the SATS-engineered strain increased by 6.03-fold. The titer of amorpha-4,11-diene, a representative terpenoid, rose by 37.77-fold to 188.45 mg/L in shake-flask fermentation and reached 13 g/L in a 5 L bioreactor. Furthermore, the modified strain exhibited enhanced production of other terpenoids. Our research indicates SATS is an effective approach for synthesizing terpenoids and other acetyl-CoA-derived compounds, demonstrating broad applicability.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109572"},"PeriodicalIF":3.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142655248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The synergistic interaction of fungi with different structural characteristics improves the leaching of lithium from lepidolite","authors":"Huaiyu Duan ,&nbsp;Xingqing Zhao ,&nbsp;Chao Xu ,&nbsp;Du Zhang ,&nbsp;Wei Gu ,&nbsp;Rucheng Wang ,&nbsp;Xiancai Lu","doi":"10.1016/j.bej.2024.109564","DOIUrl":"10.1016/j.bej.2024.109564","url":null,"abstract":"<div><div>The aim of this study was to explore the improvement of lithium leaching from lepidolite by microbial co-culture, focusing on the synergistic effect of different structural fungi in improving the leaching performance of biological systems. The results showed that in the single leaching experiment, the multicellular fungi and the unicellular yeast showed weak effects. Multicellular fungus is limited by insufficient EPS secretion, while unicellular yeast is non-mycelial organisms with weak acid production capacity and limited effect on minerals. However, in the combined leaching experiment, the interspecific collaboration promoted the synthetic and metabolic activity of the two strains, resulting in changes in the type and content of organic acids, polysaccharides, proteins and humus. The content of citric acid reached the highest value of 16.98 g·L⁻¹ at about 22 d, and the EPS secreted by unicellular yeast promoted the mycelium adhesion and mineral wrapping of multicellular fungi. The combined action of the two enhanced the effects of acidification, complexation and mycelium destruction, and improved the leaching effect. This study revealed the synergistic metabolic mechanism of lepidolite leaching by fungi with different structures, verified the effectiveness of microbial co-culture to improve the leaching rate, and provided a basis for industrial application. In addition, the use of co-culture technology has a positive impact on commercial production and environmental protection.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109564"},"PeriodicalIF":3.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142655023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of Musa balbisiana bract toward chromium removal from industrial tannery effluent: Optimization, kinetics, isotherms, regeneration, and cost estimation","authors":"Divya Baskaran ,&nbsp;Becky Miriyam I ,&nbsp;Palani R ,&nbsp;Hun-Soo Byun","doi":"10.1016/j.bej.2024.109565","DOIUrl":"10.1016/j.bej.2024.109565","url":null,"abstract":"<div><div>Eradicating chromium from industrial effluent is essential for environmental security and economic reasons. This study investigates the potency of activated <em>Musa balbisiana</em> bract biomass as a biosorbent to remove hexavalent chromium (Cr⁶⁺) from real industrial tannery effluent (ITE). The characterization study exemplifies the existence of irregular structures and excessive cavities, and the occurrence of functional groups (hydroxyl, carboxyl, esters, and alkynes) are benefitting the deposition of Cr⁶⁺ on the biosorbent. A maximum biosorption capacity of 42.75 ± 0.21 mg/g was observed at an optimum pH of 6.5, biosorbent dosage of 0.3 g, initial Cr⁶⁺ concentration of 50 mg/L, and contact time of 120 min. The validation experiment confirmed the removal efficiency of total chromium, trivalent chromium, and Cr⁶⁺ 92.56 ± 0.80 %, 98.63 ± 0.20 %, and 96.21 ± 0.50 %, respectively. Among the models, Langmuir isotherm (R²: 0.9992) and pseudo-second order (R²: 0.9999) kinetic models greatly correlate with the equilibrium data. A 2-tier membrane module was examined for continuous study and reached 88.23 ± 0.60 % Cr⁶⁺ removal. Statistical analysis was performed to confirm the significance of adsorption results. The likelihood of the desorption and regeneration of the treated biosorbent was investigated. The estimated cost per volume of ITE treated and unit of pollutant removal from ITE employing <em>Musa balbisiana</em> bract biosorbent is around $3.08/m³ and $3.75/kg.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109565"},"PeriodicalIF":3.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous endogenous partial denitrification/anammox process for low-strength wastewater treatment: Process optimization, nitrogen removal and microbial dynamics","authors":"Dong Li ,&nbsp;Fanxu Zeng ,&nbsp;Songwei Yang ,&nbsp;Yuliang Zhu ,&nbsp;Zhu Li ,&nbsp;Huiping Zeng ,&nbsp;Jie Zhang","doi":"10.1016/j.bej.2024.109568","DOIUrl":"10.1016/j.bej.2024.109568","url":null,"abstract":"<div><div>The endogenous partial denitrification process (EPD) led by glycogen-accumulating organisms (GAOs) has become an alternative to NO₂⁻ supply in mainstream anaerobic-ammonia oxidation (anammox). However, low autotrophic nitrogen removal contribution is an urgent problem that needs to be solved in simultaneous endogenous partial denitrification/anammox (EPDA) system. This study used anaerobic duration optimization to enhance the autotrophic nitrogen removal capacity of EPDA system. The results showed that the EPDA activity increased from 0.67 to 1.09 mg N/g VSS·h after anaerobic time was extended to 120 min. This significantly improved the contribution of anammox to TIN removal, increasing from 23.5 % to 61.6 %. During the phase Ⅲ, Eff.TIN of 4.5±1.8 mg/L and NRE of 92.2 %±3.0 %. The enrichment of AnAOB (<em>Candidatus Brocadia</em>) and GAOs (<em>Defluviicoccus</em>) was responsible for maintaining the stability of the EPDA process. This study provides a feasible optimization strategy for improving the contribution of autotrophic nitrogen removal in the EPDA system.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109568"},"PeriodicalIF":3.7,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of deep eutectic solvent pre-treated natural rubber gloves by Klebsiella aerogenes: A sustainable approach to rubber waste management","authors":"Harika Chittella ,&nbsp;Li Wan Yoon ,&nbsp;Suganti Ramarad ,&nbsp;Zee-Wei Lai","doi":"10.1016/j.bej.2024.109569","DOIUrl":"10.1016/j.bej.2024.109569","url":null,"abstract":"<div><div>This study investigates the potential of deep eutectic solvents (DES) to enhance the biodegradation of natural rubber gloves (NRG) by <em>Klebsiella aerogenes</em>. Choline chloride and urea (ChCl: urea) was the DES employed to pre-treat NRG at various temperatures (80°C to 140°C) and durations (0.5 h to 5 h). Pre-treated rubber (p-NRG) underwent significant physical and chemical changes, enhancing its biodegradability. Analytical techniques such as dry weight analysis, bacteria cell concentration, FTIR TGA, and SEM were used to characterize the pre-treated and biodegraded samples. The results have demonstrated a significant weight loss and structural modifications in p-NRG, with the highest degradation of 43 % observed at 140°C for 5 hours of pretreatment before biodegradation. Meanwhile, merely 17 % of weight loss was observed when pre-treatment was not employed. DES pre-treatment notably enhanced NRG biodegradability, achieving a 50.6 % weight loss when biodegradation was conducted at pH 7 and 35°C. The highest cell concentration, 0.75 g/L, was recorded in the second week of the biodegradation process. Results have indicated that the maximum protein concentration of 697.3 µg/ml, along with the highest enzyme activities for laccase and manganese peroxidase (MnP) at 0.46 ± 0.05 IU and 0.30 ± 0.05 IU respectively, were recorded in the second week of the biodegradation process. DES pre-treatment has significantly improved the biodegradability of NRG by <em>Klebsiella aerogenes</em>, offering a promising and eco-friendly solution for rubber waste management.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109569"},"PeriodicalIF":3.7,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}