Qun Wei , Dan Zhao , Ming Wang , Conghan Wang , Fu Pang , Xiangmeng Ma
{"title":"Enhanced lipid production in Chlorella vulgaris via indole-3-acetic acid salt stress in a two-stage culture for biofuels","authors":"Qun Wei , Dan Zhao , Ming Wang , Conghan Wang , Fu Pang , Xiangmeng Ma","doi":"10.1016/j.bej.2025.109795","DOIUrl":"10.1016/j.bej.2025.109795","url":null,"abstract":"<div><div>Utilizing saline wastewater for the cultivation of microalgae represents a viable approach to decrease the production costs associated with microalgae-derived biodiesel. In this study, a new method of combining plant hormones with two-stage culture was proposed to drive efficient lipid production of microalgae under salt stress. The findings indicated that the introduction of 40 mg·L<sup>−1</sup> of indole-3-acetic acid (IAA) during the initial phase resulted in optimal cell density and lipid productivity of <em>Chlorella vulgaris</em> under conditions of 10 g·L<sup>−1</sup> NaCl stress, achieving values of 65.15 × 10<sup>6</sup> cells·mL<sup>−1</sup> and 47.62 mg·L<sup>−1</sup>·d<sup>−1</sup>, respectively. In the second stage, the application of salt stress was repeated, leading to a lipid productivity of 56.17 mg·L<sup>−1</sup>·d<sup>−1</sup>, which represents an increase of 150.8 % compared to the untreated control group. Additionally, the proportion of saturated fatty acids rose to 29.26 %. Therefore, it has better combustion characteristics and stability. At the same time, the self-flocculation efficiency of microalgae also increased by 93.75 %. In addition, the regulatory mechanism of IAA on the growth and lipid accumulation of <em>Chlorella</em> under salt stress was discussed by quantitative analysis of the expression of genes related to signaling pathways and lipid biosynthesis in algal cells. This study provides ideas for microalgae to produce biodiesel and achieve sustainable development.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109795"},"PeriodicalIF":3.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Clinical isolation of breast cancer Circulating Tumor Cells with an inertial microfluidic chip with a trapezoidal cross-section","authors":"Zahra Sadeghi , Mohsen Nasr Esfahany , Hossein Salehi , Azar Baradaran","doi":"10.1016/j.bej.2025.109788","DOIUrl":"10.1016/j.bej.2025.109788","url":null,"abstract":"<div><div>In recent years, various detection and isolation methods for breast cancer have emerged. However, the lack of simple, unbiased separation techniques limits clinical applications of Circulating Tumor Cells (CTCs). This study introduces an inertial microfluidic chip with a trapezoidal cross-section for rapid, simple separation of MCF-7 CTCs from patient blood using an optimized SLA 3D printing technique. Simulations and experiments showed that increasing the slant angle enhances Dean vortices, with 1.2 mL/min as the optimal flow rate. Spiking MCF-7 cells into Phosphate-Buffered Saline (PBS) yielded a recovery rate of 88 ± 3.5 % and a purity of 99.99 %. When spiked into 7.5 mL lysed blood, the recovery was 86 ± 2.9 % and purity 94 ± 2.3 %, with over 90 % of cells remaining viable. Clinically, the chip detected MCF-7 cells in 8 out of 10 breast cancer patients (80 %), with cell counts ranging from 2 to 21 cells/mL and purities between 89 ± 1.6 % and 92 ± 5.8 %. These results confirm the chip’s high sensitivity and viability preservation. This straightforward inertial microfluidic method offers a promising alternative to traditional affinity-based CTC separation, supporting fundamental research and aiding patient treatment strategies.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109788"},"PeriodicalIF":3.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiao-Yan Fan , Ji-Gang Ma , Wen-Nian Geng , Jia-Wei Ren , Shen-Bin Cao , Qing-Liang Sun , Jun-Ru Zhao
{"title":"Shifts in microbial communities and antibiotic resistance genes in activated sludge driven by varying heterotrophic nitrifying aerobic denitrifying bacteria ratios","authors":"Xiao-Yan Fan , Ji-Gang Ma , Wen-Nian Geng , Jia-Wei Ren , Shen-Bin Cao , Qing-Liang Sun , Jun-Ru Zhao","doi":"10.1016/j.bej.2025.109790","DOIUrl":"10.1016/j.bej.2025.109790","url":null,"abstract":"<div><div>Activated sludge systems experience reduced microbial activity under low-temperature conditions, which adversely affects the biological nitrogen removal process. Heterotrophic nitrifying aerobic denitrifying bacteria (HNADB), with their strong tolerance to low temperatures, present a promising solution through bioaugmentation. This study explored the bio-enhancement of activated sludge systems using HNADB to improve nitrogen removal, and evaluated microbial community structure, functional genes, and antibiotic resistance genes (ARGs) through batch experiments with varying inoculation ratios (from 0 % to 100 %) of HNADB-enriched sludge. Higher inoculation ratios (80 % and 100 %) significantly enhanced total nitrogen (TN) removal by 32.75 % and 43.62 %, respectively, though nitrification efficiency declined. Community diversity increased, while richness decreased under higher inoculation. Abundant taxa (AT) constituted the dominant proportion in all systems and were further enriched in the high-inoculation treatments. High inoculation ratios enriched denitrifying bacteria (DNB), HNADB (<em>Thauera</em>, <em>Hyphomicrobium</em>, <em>Rhodococcus</em>), and their related functional genes, while decreasing the abundance of nitrifying microorganisms and genes. The ARGs diversity increased but abundance decreased over time. DNB and HNADB may serve as hosts for ARGs, while nitrifying microorganisms show higher sensitivity to antibiotics. This study highlighted the potential of HNADB-based inoculation to enhance nitrogen removal performance and community resilience under low-temperature conditions, providing an effective biological enhancement strategy for low-temperature wastewater treatment.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109790"},"PeriodicalIF":3.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Factors influencing the removal of extracellular resistance genes in activated sludge process","authors":"Jinyuan Xue , Mengqi Zhang , Anji Chen , Yuhan Li , Chenke Zhong , Chaoqi Chen","doi":"10.1016/j.bej.2025.109791","DOIUrl":"10.1016/j.bej.2025.109791","url":null,"abstract":"<div><div>Extracellular antibiotic resistance genes (eARGs) contribute to antimicrobial resistance spread in the environment, yet their removal mechanisms in activated sludge remain poorly understood. Here, an artificial resistant plasmid (PUC57-<em>sul</em>1) and its corresponding <em>sul</em>1 amplicon were treated in aerobic reactors under varying conditions. The removal of eARGs was significantly affected by sludge disinfection, biomass concentrations, aeration rate, and temperature. The results indicated that the influencing factors for eARGs are similar to those for small-molecular-organic compounds. At optimal conditions (5 g/L biomass concentration, 400 mL/min aeration rate, and 30°C), the removal efficiency at 12-h was 88.7 % for the amplicon and 67.4 % for the plasmid, respectively, indicating that eARG removal was largely affected by size and conformation. A transient increase of eARGs was observed when actual hospital wastewater was treated, likely due to the lysis of ARG-carrying bacteria. In undiluted hospital wastewater, the concentration of <em>sul</em>1 was reduced by 15.3 % relative to its peak concentration, even under the optimized conditions. In contrast, when the wastewater was 10-fold diluted, the concentration of <em>sul</em>1 was reduced by 81.3 %, confirming the effectiveness of the activated sludge process when toxicity was reduced. Microbial inhibition may be the key factor limiting eARG removal. These findings contribute to a better understanding of factors facilitating the degradation of eARGs, potentially guiding future strategies for improving wastewater treatment practices.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109791"},"PeriodicalIF":3.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuan Shan , Yanyan Wang , Jing Zhang , Yihan Gong , Tiantian Yao , Yihao Wang , Guanghui Chen , Deshuang Yu
{"title":"Enhancing fatty acid production from waste activated sludge through low-temperature heat treatment and biostimulant pretreatment: Performance and mechanism analysis","authors":"Yuan Shan , Yanyan Wang , Jing Zhang , Yihan Gong , Tiantian Yao , Yihao Wang , Guanghui Chen , Deshuang Yu","doi":"10.1016/j.bej.2025.109789","DOIUrl":"10.1016/j.bej.2025.109789","url":null,"abstract":"<div><div>Anaerobic fermentation of sludge is constrained by low short-chain fatty acids (SCFAs) yields and limited solubilization of organic matter. This study presents a novel approach that integrates low-temperature heat treatment with biostimulant pretreatment to enhance the recovery of SCFAs from waste activated sludge. Experimental results indicate that applying 90 °C heat treatment and 1.2 g/g TSS D-limonene without pH adjustment led to an SCFAs yield of 2664 mg/L, representing a 2.3-fold increase over the control group. Furthermore, under conditions with initial pH set to 12, the SCFAs yield peaked at 2977 mg/L by the seventh day. Mechanistic analysis revealed that the combined pretreatment effectively disrupted extracellular polymeric substances and cell walls, facilitating SCFAs production. Microbial community analysis demonstrated an enrichment of Proteobacteria, Actinobacteria, and chain-elongating bacteria, enhancing the conversion of low-value organic matter into high-value products. The results offer new perspectives for optimizing anaerobic fermentation processes to enhance organic matter recovery from sludge.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109789"},"PeriodicalIF":3.7,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Expressing and functionally evaluating anticancer bispecific antibody fragments using cyanobacterium Synechocystis sp. PCC6803 for sustainable production","authors":"Gota Kasai , Sayumi Shioya , Miku Kobe , Shunichi Kobayashi , Shouhei Takamatsu , Koki Makabe , Izumi Kumagai , Koji Sode , Ryutaro Asano","doi":"10.1016/j.bej.2025.109776","DOIUrl":"10.1016/j.bej.2025.109776","url":null,"abstract":"<div><div>Anticancer bispecific antibody (BsAb) fragments are effective therapeutic agents because of their high tissue penetration and cytotoxic effects, which can be produced in cost-effective bacterial hosts. We used the cyanobacterium <em>Synechocystis</em> sp. PCC6803 (PCC6803) as an alternative host for sustainably producing BsAb fragments from CO<sub>2</sub>. Cyanobacteria are sustainable and independent of external carbon sources such as glucose, because of producing substances via photosynthesis. Cyanobacteria can be cultured using wastewater or seawater, reducing freshwater consumption and the environmental impact of the process, suggesting that cyanobacteria can be used as a sustainable BsAb production platform. We examined the production of various antibody fragments, namely, single-chain bispecific diabody (scBsDb), single-chain fragment variable (scFv), variable domains of the heavy-chain antibody VHH, and bispecific tandem VHH (BsVHH), using PCC6803. scBsDb showed partial antigen-binding ability and no cytotoxic effects. In contrast, all scFvs and VHH had binding abilities and we prepared BsVHH up to 2.4 mg/L culture. BsVHH showed binding activity with the targets and cytotoxic effects against carcinoma, unlike scBsDb. Our results highlight the suitability of VHH and VHH-based engineered antibody fragments for using PCC6803 and indicate their potential for producing other therapeutic proteins. We report producing functional BsAb fragments using cyanobacteria. Our findings contribute to developing cyanobacterial bioprocesses for producing therapeutic BsAb fragments, expanding the possibilities for sustainably producing valuable materials from CO<sub>2</sub>.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109776"},"PeriodicalIF":3.7,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maosheng Yin , Shuiquan Chen , Meng Zang , Lin Li , Chaocheng Zhao , Xiuxia Zhang
{"title":"Enhanced biodesulfurization of recalcitrant organosulfur compounds by a newly identified Gordonia alkanivorans JT-2 in the presence of mycolic acid","authors":"Maosheng Yin , Shuiquan Chen , Meng Zang , Lin Li , Chaocheng Zhao , Xiuxia Zhang","doi":"10.1016/j.bej.2025.109779","DOIUrl":"10.1016/j.bej.2025.109779","url":null,"abstract":"<div><div>With the increasing stringency of global regulations on the sulfur content of petroleum products, the development of efficient desulfurization technologies has become imperative. While biodesulfurization offers an eco-friendly alternative to hydrodesulfurization, its industrial adoption remains hindered by (i) poor degradation of alkylated DBTs/BTs and (ii) inefficient oil-water phase mass transfer. In this study, we identified a novel strain, <em>Gordonia alkanivorans JT-2</em>, demonstrating enhanced biodesulfurization performance, attributed to its production of mycolic acid, which improves hydrophobicity and mass transfer efficiency. Genomic analysis revealed key desulfurization genes (<em>dszA</em>, <em>dszB</em>, <em>dszC</em>, and <em>dszD</em>) and mycolic acid biosynthesis genes (<em>fas</em>, <em>pks13</em>, <em>kasAB</em>, <em>fabD</em>, and <em>fadD32</em>). Biodesulfurization experiments showed significant sulfur removal, reducing sulfur content in model oil to 35.7 ± 5.9 mg/L (DBT), 55.2 ± 7.9 mg/L (4-MDBT), 63.1 ± 5.7 mg/L (3-MBT), and 73.7 ± 3.8 mg/L (4,6-DMDBT) within 5 days. Protein interaction network analysis further illustrated the interplay between lipid biosynthesis and sulfur metabolism, underscoring the metabolic adaptability of strain JT-2. This work addresses a critical limitation in existing biodesulfurization technologies by improving mass transfer efficiency and broadening substrate range, positioning <em>G. alkanivorans</em> JT-2 as a promising candidate for industrial biodesulfurization applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109779"},"PeriodicalIF":3.7,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ruihong Sun , Qingzhong Wang , Shuqin Shao , Shanshan Zhang , Ying Wang
{"title":"Degradation of malachite green by a novel salt-tolerant Vibrio natriegens: Optimal degradation conditions, mechanisms, and safety assessment","authors":"Ruihong Sun , Qingzhong Wang , Shuqin Shao , Shanshan Zhang , Ying Wang","doi":"10.1016/j.bej.2025.109775","DOIUrl":"10.1016/j.bej.2025.109775","url":null,"abstract":"<div><div>Synthetic dyes, such as malachite green (MG), are widely used in the textile industry but pose significant environmental risks due to their toxicity. In this study, a highly efficient salt-tolerant bacterial strain, <em>Vibrio natriegens</em> SWS5, was isolated and identified through morphological, physiological, biochemical, and 16S rRNA gene sequence analyses. Systematic investigations were conducted to optimize degradation conditions, analyze enzyme activities, elucidate degradation pathways, and assess detoxification effects. The optimal degradation conditions were determined as follows: peptone 7.5 g/L, NaCl 20 g/L, Fe²⁺ 0.04 g/L, pH 7, temperature 30 °C, and shaking speed 220 rpm. Under these conditions, SWS5 achieved 97.88 % degradation of MG (100 mg/L) within 168 hours. After MG added, enzyme activity assays revealed in dye-decolorizing peroxidase (823 U/L), laccase (358 U/L), and manganese peroxidase (0.204 U/L) activities. HPLC-MS analysis identified key intermediates, enabling the proposal of two potential MG degradation pathways. Toxicity assessments using microbial tests and zebrafish models demonstrated a significant reduction in the toxicity of degradation products. Transcriptome sequencing provided novel insights into the regulatory pathways of MG degradation, offering a theoretical foundation for the bioremediation of MG contaminated wastewater. <em>Vibrio natriegens</em> was demonstrated for the first time to degrade triphenylmethane dyes, especially MG, in high-salinity environments. This study highlights the potential of <em>Vibrio natriegens</em> SWS5 as an efficient and eco-friendly solution for dye wastewater treatment.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109775"},"PeriodicalIF":3.7,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Feng Zhen , Hengbing Zhou , Linpei Han , Yifan Zhang , Yuwan Pang , Tao Xing , Lei Li
{"title":"Impacts of the combined application of digestate-based compost and biochar on the heavy metal Cd distribution and stress in the plant-soil system","authors":"Feng Zhen , Hengbing Zhou , Linpei Han , Yifan Zhang , Yuwan Pang , Tao Xing , Lei Li","doi":"10.1016/j.bej.2025.109773","DOIUrl":"10.1016/j.bej.2025.109773","url":null,"abstract":"<div><div>In this study, a pot experiment was conducted to evaluate the effects of digestate-based compost, chemical fertilizer, and their combination with biochar on heavy metal distribution, plant growth, and soil microbial communities. The experimental results demonstrated that the combined application of digestate-based compost and biochar (OFB group) promoted heavy metal immobilization in soil and enhanced plant growth, while reducing the accumulation of heavy metals in plants. Oxidizable and residual Cd levels in the OFB group increased by 14.90 % and 17.60 % compared to the control group. The biomass of Chinese cabbage was 384 % higher than that of the control group, and its Cd content decreased by 57.90 % compared to the control group. The addition of digestate-based compost to the soil increased soil organic matter, total nitrogen, total phosphorus, total potassium, and electrical conductivity (p < 0.05), along with the relative abundance of beneficial microorganisms such as <em>Bacillus</em>, <em>norank_f__norank_o__Actinomarinales</em>, <em>Chryseolinea</em>, <em>Saccharomonospora</em>, <em>Pseudomonas,</em> and <em>norank_f__BIrii41</em>. Biochar played a significant role in minimizing the migration of heavy metals to plants. The study demonstrated that the combined application of digestate-based compost and biochar reduces heavy metal stress, enhances soil fertility, and promotes biomass growth, offering potential solutions for the sustainable management of cadmium-contaminated soil and the application of digestate-based compost.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109773"},"PeriodicalIF":3.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maxime Maton , Baptiste Leroy , Alain Vande Wouwer
{"title":"A constructive bottom-up approach for the elaboration of metabolic networks: Case study of photosynthetic cyanobacteria Arthrospira spirulina platensis PCC 8005","authors":"Maxime Maton , Baptiste Leroy , Alain Vande Wouwer","doi":"10.1016/j.bej.2025.109770","DOIUrl":"10.1016/j.bej.2025.109770","url":null,"abstract":"<div><div>Mathematical modeling has proven to be a highly effective tool for understanding microbial metabolism for which in-silico and experimental studies help to quantify intracellular mechanisms and pave the way for optimizing the production of molecules of interest. In that context, the development of metabolic networks turns out to be particularly interesting despite the challenges underlying their reconstruction. While the elaboration of genome-scale networks is computationally costly, small networks are often oversimplified and important biological mechanisms might be omitted, which limits their use in industrial applications. For this purpose, this study proposes a constructive bottom-up approach for the identification of metabolic networks of intermediate size, typically comprised of a couple of hundred reactions. It combines basic biological knowledge and a series of constraint-based methods in an iterative strategy, enabling the refinement of the network definition. The network is first validated using in-silico data, and subsequently refined using experimental data to enhance its biological relevance. Several case studies have been addressed to assess the efficiency of the methodology, and this paper focuses on the modeling of photosynthetic cyanobacteria <em>Arthrospira</em> sp. PCC 8005. The procedure is effective and provides promising results and metabolic analyses show consistent predictive capabilities of the network, in concordance with existing studies.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109770"},"PeriodicalIF":3.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}