Biochemical Engineering Journal最新文献

Simultaneous removal of nitrate, zinc, and bisphenol A by an iron-modified biochar composite mycelial pellet bioreactor: Optimization and microbial mechanisms 铁修饰生物炭复合菌丝球生物反应器同时去除硝酸盐、锌和双酚A：优化和微生物机制

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-07-01 Epub Date: 2026-03-10 DOI: 10.1016/j.bej.2026.110161

Yinan Wang , Jiayao Ma , Junfeng Su , Yu Liu , Li Luo , Yihan Bai , Xuan Li

{"title":"Simultaneous removal of nitrate, zinc, and bisphenol A by an iron-modified biochar composite mycelial pellet bioreactor: Optimization and microbial mechanisms","authors":"Yinan Wang , Jiayao Ma , Junfeng Su , Yu Liu , Li Luo , Yihan Bai , Xuan Li","doi":"10.1016/j.bej.2026.110161","DOIUrl":"10.1016/j.bej.2026.110161","url":null,"abstract":"<div><div>In this study, iron-modified biochar composite mycelial pellets (CMPs) were utilized to immobilize the Fe<sup>2 +</sup> oxidizing denitrifying bacterial strain <em>Zoogloea</em> sp. FY-6, with the aim of simultaneous removal of nitrate (NO<sub>3</sub><sup>-</sup>-N), zinc (Zn<sup>2+</sup>) and bisphenol A (BPA). Under conditions of 8 h hydraulic retention time (HRT), pH 7.0, and influent concentrations of 25.0 mg L<sup>−1</sup> NO<sub>3</sub><sup>-</sup>-N, 1.0 mg L<sup>−1</sup> Zn<sup>2+</sup>, and 1.0 mg L<sup>−1</sup> BPA, the bioreactor reached its best performance, with NO<sub>3</sub><sup>-</sup>-N, Zn<sup>2+</sup>, and BPA removal efficiencies of 87.2%, 92.4%, and 94.4%, respectively. The experimental results demonstrated that the addition of iron-modified biochar can enhance denitrification. The removal of Zn<sup>2+</sup> and BPA was primarily attributed to adsorption and coprecipitation mediated by biogenic precipitates formed during bioreactor operation. Through high-throughput sequencing and metabolic pathway analysis, it was found that <em>Bacteroidia</em>, <em>Alphaproteobacteria</em>, and <em>Clostridia</em> were key contributors to denitrification and contaminant degradation. Metabolic pathway analysis further elucidated the synergistic relationships between these microbial communities and the iron-modified biochar. This research provides new insights into the treatment of complex industrial wastewater using multifunctional microbial systems.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"231 ","pages":"Article 110161"},"PeriodicalIF":3.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microgravity mutagenesis in E. coli: A molecular mechanism for high-yield cadaverine production 大肠杆菌微重力诱变：一种高产尸胺的分子机制

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-07-01 Epub Date: 2026-03-02 DOI: 10.1016/j.bej.2026.110147

Guangqiang Shui , Beiya Zhou , Jun Ma , Disen Zhang , Ying Bi , Yuhong Huang

{"title":"Microgravity mutagenesis in E. coli: A molecular mechanism for high-yield cadaverine production","authors":"Guangqiang Shui , Beiya Zhou , Jun Ma , Disen Zhang , Ying Bi , Yuhong Huang","doi":"10.1016/j.bej.2026.110147","DOIUrl":"10.1016/j.bej.2026.110147","url":null,"abstract":"<div><div>Cadaverine serves as a monomer with significant potential in the industrial synthesis of polyamides, particularly nylon 5X. However, its broad application remains constrained by low microbial productivity and inherent cellular toxicity. Currently, the molecular mechanisms governing cadaverine production and tolerance in <em>E. coli</em> remain incompletely elucidated. In this study, we developed two engineered strains, Δ<em>E. coli</em> LdcEt-D8 and Δ<em>E. coli</em> LdcEt-MG-I6, through microgravity mutagenesis and adaptive laboratory evolution. These strains exhibited remarkable performance enhancements: cadaverine concentration (g L<sup>−1</sup>) in the whole-cell catalytic reaction increased by 191% and 412%, respectively, while cadaverine tolerance rose by 139% and 193%, relative to the parental strain <em>E. coli</em> LdcEt. Whole genome and transcriptomic analyses revealed that enhanced central carbon metabolism pathway contributed to increased cadaverine production. Concurrent upregulation of amino acid metabolism pathway, fatty acid synthesis pathway, and genetic information repair pathway correlated strongly with improved cadaverine tolerance. Validation experiments on mutant genes confirmed that individual overexpression of <em>purA</em>, <em>accC</em>, <em>holB</em>, <em>cysM</em>, and <em>prps</em> in <em>E. coli</em> BL21(DE3) consistently enhanced cadaverine production, underscoring the indispensable role of mutant genes in the biosynthesis pathway. Collectively, these findings provide insight into the molecular mechanism behind the improved production in mutant strains, as well as decoding the transcriptomic landscape, which provides key targets for advancing whole-cell catalytic synthesis of cadaverine in <em>E. coli</em>.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"231 ","pages":"Article 110147"},"PeriodicalIF":3.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methodological framework for fused filament fabrication of 3D-printed beads for cell immobilization in bioprocesses 用于生物过程中细胞固定化的3d打印珠的熔丝制造方法框架

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-07-01 Epub Date: 2026-03-05 DOI: 10.1016/j.bej.2026.110146

Ricardo Gonzalo Ramírez Brenes , Rubén Ruiz Simón , Isabella Maria Tenório Soares Santos , Victoria E. Santos Mazorra , Ninoska Bojorge Ramírez , Nei Pereira Jr

{"title":"Methodological framework for fused filament fabrication of 3D-printed beads for cell immobilization in bioprocesses","authors":"Ricardo Gonzalo Ramírez Brenes , Rubén Ruiz Simón , Isabella Maria Tenório Soares Santos , Victoria E. Santos Mazorra , Ninoska Bojorge Ramírez , Nei Pereira Jr","doi":"10.1016/j.bej.2026.110146","DOIUrl":"10.1016/j.bej.2026.110146","url":null,"abstract":"<div><div>This study presents an innovative methodology for fabricating polymeric beads via 3D printing to enhance cell immobilization strategies in bioprocess engineering. Using fused filament fabrication (FFF), beads composed of acrylonitrile butadiene styrene (ABS) and polylactic acid (PLA) were fabricated with precisely tailored geometries, enabling the systematic evaluation of how material type and internal design influence both manufacturing feasibility and structural stability. The methodology demonstrates high reproducibility, dimensional accuracy and flexibility, allowing researchers to fabricate immobilization beads adapted to specific bioprocess conditions. In contrast to conventional immobilization matrices, this FFF-based approach offers a scalable, cost-effective and customizable alternative, capable of producing complex porous architectures that promote microbial adhesion and mass transfer. The method stands out for extending additive manufacturing applications beyond enzyme immobilization toward whole-cell biocatalyst systems, providing a valuable framework for future biotechnological process development.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"231 ","pages":"Article 110146"},"PeriodicalIF":3.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of high-valued D-allulose from sucrose via in vitro multi-enzyme cascade 体外多酶级联酶法从蔗糖制备高价值D-allulose

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-07-01 Epub Date: 2026-03-08 DOI: 10.1016/j.bej.2026.110158

Tian Xiao , Wei Zhao , Ruijin Yang , Xiaomei Lyu

引用次数: 0

Hydrodynamic optimization of a newly designed and fabricated U-Loop bioreactor using Taguchi–ANOVA analysis 新设计和制造的U-Loop生物反应器流体动力学优化的田口方差分析

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-07-01 Epub Date: 2026-02-23 DOI: 10.1016/j.bej.2026.110141

Sohrab Valadbeigy , Mohammad Naghashzadegan , Reza H. Sajedi , Asma Chegeni

{"title":"Hydrodynamic optimization of a newly designed and fabricated U-Loop bioreactor using Taguchi–ANOVA analysis","authors":"Sohrab Valadbeigy , Mohammad Naghashzadegan , Reza H. Sajedi , Asma Chegeni","doi":"10.1016/j.bej.2026.110141","DOIUrl":"10.1016/j.bej.2026.110141","url":null,"abstract":"<div><div>This study focuses on the design and hydrodynamic optimization of a newly engineered U-loop bioreactor aimed to improve mixing performance in viscous media for Single-Cell Protein (SCP) production and also compared the Statistical and Practical Optimum which considers industrial condition. Three major innovations were implemented: first, the reduction of pump power to a practical optimum value of 8600 W/m<sup>3</sup> and improve mixing efficiency; second, the proposal of optimal operational parameters, including the practical optimum culture medium viscosity of 0.045 Pa.s (0.2-wt% CMC), as an indicator of high SCP concentrations, the optimal inoculum (400 g/Lit), and the optimal static-mixer position ( close to degassing tank inlet); and third, the design and testing of a custom swirl static mixer to enhance circulation and mixing. The effects of these parameters were evaluated using a Taguchi L9 experimental design and ANOVA. The results showed that medium viscosity, was the dominant factor affecting mixing time, followed by pump power and static-mixer position. The experimentally validated statistical optimum configuration of 0-wt% CMC, 96 W pump power, 45-cm static-mixer placement, and 400 g/Lit inoculum results in an average mixing time of 9.9 s, in close agreement with the Taguchi prediction. This study provides a mechanistic and statistically grounded framework for optimizing U-loop bioreactors, offering a solid foundation for SCP production.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"231 ","pages":"Article 110141"},"PeriodicalIF":3.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ferric pyrophosphate-activated N and S self-doped keratin biochar derived from rabbit hair enables efficient Cr(VI) removal: Synergistic effect of Fe(Ⅱ) with N and S 从兔毛中提取的焦磷酸铁活化的N和S自掺杂角蛋白生物炭能够高效去除Cr(VI): Fe（Ⅱ）与N和S的协同效应

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-07-01 Epub Date: 2026-03-09 DOI: 10.1016/j.bej.2026.110160

Zhenjie Chen , Huan Yang , Zelun Su , Xiaoqing Wang , Xiangyun Chen , Na Pang

{"title":"Ferric pyrophosphate-activated N and S self-doped keratin biochar derived from rabbit hair enables efficient Cr(VI) removal: Synergistic effect of Fe(Ⅱ) with N and S","authors":"Zhenjie Chen , Huan Yang , Zelun Su , Xiaoqing Wang , Xiangyun Chen , Na Pang","doi":"10.1016/j.bej.2026.110160","DOIUrl":"10.1016/j.bej.2026.110160","url":null,"abstract":"<div><div>The removal of Cr(VI) from wastewater by biochar is limited by the surface chemical structure. The doping structure is the key for biochar to improve the surface chemical properties. In this study, the N,S,Fe self-doped biochar for Cr(VI) removal was prepared by pyrolysis and activation by Fe<sub>4</sub>(P<sub>2</sub>O<sub>7</sub>)<sub>3</sub> of waste rabbit hair. The results show that Fe-RBC successfully retains the oxygen-containing and N functional groups in keratin, simultaneously generating <span><math><mrow><msubsup><mrow><mi>SO</mi></mrow><mrow><mn>4</mn></mrow><mrow><mn>2</mn><mo>−</mo></mrow></msubsup><mspace></mspace></mrow></math></span>and<span><math><mrow><mspace></mspace><msup><mrow><mi>S</mi></mrow><mrow><mn>2</mn><mo>−</mo></mrow></msup></mrow></math></span>, and introducing Fe<sup>2 +</sup> . The findings of Cr(VI) removal study indicated that the 1.2 g/L biochar exhibited a removal efficiency of approximately 98.33% for Cr(VI) in wastewater within 6 h. The XPS and CV results show that the carbon skeleton constitutes a stable conductive and adsorption substrate. S participates in and promotes the cycling of Fe (II) and Fe (III), and then serves as a direct electron transfer medium to reduce Cr(VI). The significantly enhanced aromatic π electron cloud obtained by graphite-n contributing electrons to the carbon skeleton π system provides adsorption and fixation sites for the reduction product Cr(III) through cation -π interactions. This research fully exploited the natural structural advantages of protein fibers and achieved <em>in-situ</em> controllable preparation of heteroatom self-doped biochar.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"231 ","pages":"Article 110160"},"PeriodicalIF":3.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergy between oxygenation and co-culture with liver sinusoidal endothelial cells drives expansion of functional liver organoids 氧合与肝窦内皮细胞共培养之间的协同作用驱动功能性肝类器官的扩张

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-07-01 Epub Date: 2026-03-03 DOI: 10.1016/j.bej.2026.110148

Takeshi Katsuda , Tia Utami , Ya Gong , Fumiya Tokito , Taketomo Kido , Kumiko Matsunaga , Atsushi Miyajima , Masaki Nishikawa , Yasuyuki Sakai

{"title":"Synergy between oxygenation and co-culture with liver sinusoidal endothelial cells drives expansion of functional liver organoids","authors":"Takeshi Katsuda , Tia Utami , Ya Gong , Fumiya Tokito , Taketomo Kido , Kumiko Matsunaga , Atsushi Miyajima , Masaki Nishikawa , Yasuyuki Sakai","doi":"10.1016/j.bej.2026.110148","DOIUrl":"10.1016/j.bej.2026.110148","url":null,"abstract":"<div><div>Induced pluripotent stem cell (iPSC)-derived hepatocyte-like cells (HLCs) hold great promise for drug discovery and cell therapy, but their immature phenotypes and limited proliferative capacity remain major challenges. We previously showed that direct oxygenation using an oxygen-permeable membrane enhances the differentiation of iPSC-derived liver progenitor cells (LPCs), although proliferation ceases during the terminal phase of maturation. Here, we demonstrate that combining direct oxygenation with co-culture of iPSC-derived liver sinusoidal endothelial cells (LSECs) overcomes this limitation. Under oxygenated conditions, co-cultured organoids exhibited robust increase in volume, whereas either oxygenation or co-culture alone was insufficient to support growth, indicating a synergistic effect. Importantly, oxygenated co-culture organoids maintained hepatic maturation comparable to oxygenated LPC monoculture organoids, based on albumin secretion and cytochrome P450 metabolic activities. Furthermore, oxygenation promoted spatial reorganization in co-cultured organoids, with LPCs occupying the center and LSECs forming a peripheral layer, suggesting improved architectural fidelity to the liver microenvironment. Together, this approach enables the scalable generation of highly mature liver organoids with enhanced structural and functional fidelity.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"231 ","pages":"Article 110148"},"PeriodicalIF":3.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing biomass and energy feedstock production from algal-bacterial biofilms treating swine wastewater: The role of organic carbon sources 优化藻类-细菌生物膜处理猪废水的生物质和能源原料生产：有机碳源的作用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-07-01 Epub Date: 2026-03-08 DOI: 10.1016/j.bej.2026.110149

Qi Zhao , Tianxiang Lu , Jie Li , Xinglong Zhou , Chongqing Xu , Mingming Song , Ze Yu

{"title":"Optimizing biomass and energy feedstock production from algal-bacterial biofilms treating swine wastewater: The role of organic carbon sources","authors":"Qi Zhao , Tianxiang Lu , Jie Li , Xinglong Zhou , Chongqing Xu , Mingming Song , Ze Yu","doi":"10.1016/j.bej.2026.110149","DOIUrl":"10.1016/j.bej.2026.110149","url":null,"abstract":"<div><div>This study establishes a novel strategy for the valorization of swine wastewater by co-producing lipid-rich and protein-rich microbial biomass using organic carbon-amended algal-bacterial biofilms. Among four carbon sources in the immobilized biofilm system, sodium acetate (C/N = 18) proved optimal, achieving a biomass productivity of 45.43 g/m²/d, which was 5.71 times greater than suspended co-cultures. Crucially, this system significantly enhanced the content and yield of valuable bioproducts, with lipid and protein contents reaching 30.46% and 42.39%, corresponding to yields of 110.70 g/m² and 154.06 g/m², respectively. The robust system performance also ensured high removal efficiencies for nutrients (NH₄⁺-N: 99.44%; TN: 98.02%; TP: 91.55%) and the antibiotic sulfamethazine (SM2: 75.63%), underscoring its efficacy in simultaneous wastewater treatment. Mechanistic investigations attributed the superior performance to sodium acetate-driven metabolic upregulation. This was marked by the extracellular accumulation of <span>L</span>-glutamate — a central metabolite in nitrogen assimilation — and hydrophobic amino acids, which collectively indicate a enhanced metabolic flux toward the synthesis of cellular proteins and lipids. This work demonstrates a sustainable, carbon-enhanced biofilm platform for the simultaneous recovery of energy (biodiesel precursor) and feed (protein) resources from swine wastewater.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"231 ","pages":"Article 110149"},"PeriodicalIF":3.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced recovery of phenolic compounds from vegetable oil processing wastewater through a synergistic emulsion liquid membrane process 协同乳状液膜法提高植物油加工废水中酚类化合物的回收率

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-07-01 Epub Date: 2026-03-10 DOI: 10.1016/j.bej.2026.110163

Norela Jusoh , Izzat Naim Shamsul Kahar , Norasikin Othman , Norul Fatiha Mohamed Noah , Shuhada A. Idrus-Saidi , Muhammad Abbas Ahmad Zaini

{"title":"Enhanced recovery of phenolic compounds from vegetable oil processing wastewater through a synergistic emulsion liquid membrane process","authors":"Norela Jusoh , Izzat Naim Shamsul Kahar , Norasikin Othman , Norul Fatiha Mohamed Noah , Shuhada A. Idrus-Saidi , Muhammad Abbas Ahmad Zaini","doi":"10.1016/j.bej.2026.110163","DOIUrl":"10.1016/j.bej.2026.110163","url":null,"abstract":"<div><div>Vegetable oil processing wastewater represents an underutilised bioresource rich in phenolic compounds (PCs) with high antioxidant potential. In this study, a synergistic emulsion liquid membrane (SELM) process was investigated to extract and recover PCs from palm oil mill sterilisation condensate by using a synergistic formulation of diluent (palm/sunflower oil), carrier (Aliquat 336/D2EHPA), surfactant (Span 80/Tween 80), and stripping agent (NaOH/Na<sub>2</sub>CO<sub>3</sub>). A two-level factorial design was first employed to screen the key operational parameters influencing the extraction performance. Then, four most significant parameters (mixed carrier and stripping agent concentrations, agitation speed, feed-to-emulsion ratio) were optimised by using Box-Behnken design to maximise the extraction performance. The effects of octanol as a modifier and feed phase concentration were studied to improve recovery performance. Under the optimal conditions of Aliquat 336/D2EHPA (0.2855/0.0023 M), NaOH/Na<sub>2</sub>CO<sub>3</sub> (0.5129/0.0615 M), an agitation speed of 263 rpm and a feed-to-emulsion ratio of 2.94:1, the SELM process achieved an extraction performance of 91.2%. Modification of SELM with 5% w/v of octanol resulted in almost 80% recovery and 9.2 times solute enrichment. The results also demonstrated that the optimum formulation of SELM process remained effective up to 340 milligram gallic acid equivalents per liter (mg GAE/L) of feed phase concentration. These findings indicate that SELM process is promising for the valorisation of agro-industrial wastewater within resource recovery frameworks.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"231 ","pages":"Article 110163"},"PeriodicalIF":3.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Redirecting formic acid flux and balancing redox for high-yield succinic acid production in Actinobacillus succinogenes 琥珀酸放线杆菌甲酸通量重定向和氧化还原平衡高产琥珀酸生产

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-07-01 Epub Date: 2026-03-08 DOI: 10.1016/j.bej.2026.110159

Yuan Tian, Chunmei Chen, Xinglan Shi, Dan Wu, Pengcheng Chen, Pu Zheng

{"title":"Redirecting formic acid flux and balancing redox for high-yield succinic acid production in Actinobacillus succinogenes","authors":"Yuan Tian, Chunmei Chen, Xinglan Shi, Dan Wu, Pengcheng Chen, Pu Zheng","doi":"10.1016/j.bej.2026.110159","DOIUrl":"10.1016/j.bej.2026.110159","url":null,"abstract":"<div><div>Succinic acid (SA) is an important organic dicarboxylic acid with broad applications in in chemical, pharmaceutical, and food industries. <em>Actinobacillus succinogenes</em> is a natural succinate producer considered a promising industrial strain. However, the formation of by-products such as formic acid (FA) and acetate acid (AA) during SA biosynthesis remains one of the major challenges. In this study, we focused on the role of FA metabolic branch in <em>A. succinogenes</em>. By inactivation of pyruvate formate-lyase using <em>A. succinogenes</em> base editors CBE, the strain that inhibited FA pathway named Δ<em>pflB</em> was obtained, and its growth, SA production, as well as intracellular nicotinamide adenine dinucleotide (NADH) were investigated. Subsequently, strategies were tried to restore NADH regeneration using overexpression of malate dehydrogenase(<em>mdh</em>), transhydrogenase(<em>pntAB</em>), and formate dehydrogenase(<em>fdh</em>) to regulate the intracellular NADH levels and NADH/NAD⁺ ratio of strain Δ<em>pflB</em>. We found that FA branch of <em>A. succinogenes</em> was critical for growth metabolic and redox balance in SA biosynthesis. Finally, through adaptive laboratory evolution to optimize the growth of strain Δ<em>pflB</em>, an evolved strain G100 with growth advantages was obtained after 100 generations. Compared with the parental strain, its SA yield increased by 37.2% and 39.2% in shake-flask and 3 L fermenter, respectively. Meanwhile, the intracellular NADH levels and the NADH/NAD⁺ ratio after evolution was significant adjusted during the fermentation process. The Δ<em>pflB</em> strain shows potential for industrial applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"231 ","pages":"Article 110159"},"PeriodicalIF":3.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0