Biochemical Engineering Journal最新文献

Nonenzymatic glucose sensing using ultrafine copper halide catalyst integrated with electrochemical and microcontroller devices 利用超细卤化铜催化剂集成电化学和微控制器器件的非酶促葡萄糖传感

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-14 DOI: 10.1016/j.bej.2025.109830

Chandan Saha, Pooja Kumari, Kaushik Mallick

{"title":"Nonenzymatic glucose sensing using ultrafine copper halide catalyst integrated with electrochemical and microcontroller devices","authors":"Chandan Saha, Pooja Kumari, Kaushik Mallick","doi":"10.1016/j.bej.2025.109830","DOIUrl":"10.1016/j.bej.2025.109830","url":null,"abstract":"<div><div>Glucose detection is important in the field of clinical medicine, particularly when it comes to diagnosing and managing diabetes. Recently there has been a surge of interest in developing non-enzymatic glucose sensors. The advancements in non-enzymatic glucose sensors have shown significant progress, making them a focal point of research and development in diagnostics. This study presents the synthesis, characterization and application of polyaniline-stabilized copper iodide nanoparticles for glucose sensing. The copper iodide nanoparticles were synthesized through a modified <em>in-situ</em> polymerization and composite formation route. X-ray diffraction analysis verified the formation of cubic copper iodide (γ-CuI), while transmission electron microscopy demonstrated that particles were distributed within the polymeric matrix, ranging within the size from 4 to 12 nm. X-ray photoelectron spectroscopy and Raman spectroscopy further validated the formation of copper (I) iodide and polyaniline. The catalyst-modified electrode was evaluated in a three-electrode setup and demonstrated enhanced electrocatalytic activity for glucose oxidation under alkaline media. A microcontroller-based glucose sensor, integrating an Arduino Uno R4 Wi-Fi module, was developed for real-time monitoring, with data transmission to the ThingSpeak cloud platform. The system exhibited a stable and reproducible response, highlighting the potential of synthesized material as a cost-effective and efficient material for non-enzymatic glucose sensing.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109830"},"PeriodicalIF":3.7,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Fe3O4@biochar on the methanogenesis process under different ammonia nitrogen level Fe3O4@biochar对不同氨氮水平下产甲烷过程的影响

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-11 DOI: 10.1016/j.bej.2025.109826

Lu Di , Fang Wang , Taili Dong , Xia Wang , Deli Zhang , Yongjun Li , Xianfa Sun , Weiming Yi

{"title":"Effect of Fe3O4@biochar on the methanogenesis process under different ammonia nitrogen level","authors":"Lu Di , Fang Wang , Taili Dong , Xia Wang , Deli Zhang , Yongjun Li , Xianfa Sun , Weiming Yi","doi":"10.1016/j.bej.2025.109826","DOIUrl":"10.1016/j.bej.2025.109826","url":null,"abstract":"<div><div>Ammonia inhibition can have toxic effects on anaerobic digestion (AD) and is detrimental to smooth operation of AD. To enhance methane production of the AD under ammonia inhibition, this study investigated the effect of addition of Fe<sub>3</sub>O<sub>4</sub>@biochar on the methanogenesis process of AD at different ammonia nitrogen (AN) level. Fe<sub>3</sub>O<sub>4</sub>@biochar significantly enhanced methane production by 11.3–183.6 % at NH<sub>4</sub><sup>+</sup> -N concentrations from 1200 to 6000 mg/L. Microbial community analysis revealed that Fe<sub>3</sub>O<sub>4</sub>@biochar alleviated the inhibition via promoting the enrichment of ammonia-tolerant flora associated with electron transfer and upregulating the expression of related genes. The enriched unclassified <em>Clostridiales</em> degrade acetic acid and could be involved in electron transfer with <em>Methanosarcina</em> as potential electron transfer partner. Meanwhile, the high expression of methylenetetrahydrofolate cyclohydrogenase and methylenetetrahydrofolate dehydrogenase in the Wood-Ljungdahl pathway further demonstrated that the increase of methane yield under ammonia inhibition was associated with the enhancement of the SAO-HM pathway. This study provided theoretical guidance for the regulation of AD under different concentrations of ammonia inhibition.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109826"},"PeriodicalIF":3.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bark as the optimal filler for the start-up phase of deodorizing bio-drip filters under actual farm conditions 在实际农场条件下，树皮作为除臭生物滴滤启动阶段的最佳填料

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-11 DOI: 10.1016/j.bej.2025.109825

Yudie Mao , Yue Jian , Chuan Huang , Wenlai Xu , Muhammad Younas , Renli Qi , Jiaming Zhu , Jing Wang , Kun Tian

{"title":"Bark as the optimal filler for the start-up phase of deodorizing bio-drip filters under actual farm conditions","authors":"Yudie Mao , Yue Jian , Chuan Huang , Wenlai Xu , Muhammad Younas , Renli Qi , Jiaming Zhu , Jing Wang , Kun Tian","doi":"10.1016/j.bej.2025.109825","DOIUrl":"10.1016/j.bej.2025.109825","url":null,"abstract":"<div><div>Biological trickling filter (BTF) is a commonly used deodorization technique in pig farms. However, filler selection under actual operational conditions is often limited, as most studies still focus on laboratory-scale experiments. Here, we compared the effects of five fillers (bark, straw, moving bed biofilm reactor packing (MBBR), grit, and polyester fiber) on the deodorization performance in the start-up phase of BTF in actual farming conditions. Bark has the best deodorizing properties. The deodorisation efficiency followed the order: bark > straw > MBBR > grit > polyester fiber. The removal rate of volatile organic compounds reaches 40–65 %, 100 % removal of malodor can be realized. In terms of ammonia removal, bark (90 %) and straw (91 %) showed significant advantages; H<sub>2</sub>S was completely removed except for polyester fibers. The particulate removal efficiency of MBBR was significantly lower than that of other fillers. Pressure drop stability followed the order: bark (40 Pa/d) < grit = polyester fiber (50 Pa/d) < MBBR (60 Pa/d) < straw (80 Pa/d). In addition, pressure drop was positively correlated with odor and VOCs removal. SEM characterization and biomass analysis revealed that the biomass of straw filler (230 CFU/mL) was significantly higher than that of other fillers. However, the correlation with deodorisation performance was weak. Our results provides a cost-effective and efficient solution for odor control in large-scale pig farms.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109825"},"PeriodicalIF":3.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioconversion of ginsenoside Rb1 to CK by co-immobilizing snailase and β-glycosidase on Zr-MOF prepared in the deep eutectic solvent 在深共熔溶剂中制备的Zr-MOF上共固定蜗牛酶和β-糖苷酶，使人参皂苷Rb1转化为CK

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-09 DOI: 10.1016/j.bej.2025.109824

Chunxiao Cui, Xiaojun Wang, Runze Gao, Shuqi Luo, Zhansheng Wu

{"title":"Bioconversion of ginsenoside Rb1 to CK by co-immobilizing snailase and β-glycosidase on Zr-MOF prepared in the deep eutectic solvent","authors":"Chunxiao Cui, Xiaojun Wang, Runze Gao, Shuqi Luo, Zhansheng Wu","doi":"10.1016/j.bej.2025.109824","DOIUrl":"10.1016/j.bej.2025.109824","url":null,"abstract":"<div><div>Zirconium-based metal-organic frameworks (Zr-MOF) are stable and easily functionalized, yet their synthesis has issues like poor environmental friendliness and restricted mass transfer, limiting applications. In this study, we used choline-based deep eutectic solvents (DES) instead of traditional solvents, with propionic acid (PA) as a modifier. In just 6 h, we successfully created a spherical UNP-66 biocomposite with a defect number of 0.7719. We then prepared an immobilized enzyme by covalently attaching snailase (Sna) and β-glycosidase (β-G) to the carrier using glutaric dialdehyde. It had an enzyme-loading capacity of 158 mg/g. DES sped up the synthesis and reduced enzyme-activity loss. The immobilized enzyme showed good thermal, pH, and storage stability. It efficiently catalyzed Rb1 to CK conversion, with a CK yield of 77.23 %. After 6 cycles, it retained 72.82 % of its initial relative enzyme activity. This DES-based defective MOF synthesis method has significant advantages, opening a new path for green, efficient preparation of high-value natural products.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109824"},"PeriodicalIF":3.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A kinetic model for the solvent-free lipase-catalyzed acetylation of phlorizin with triacetin 无溶剂脂肪酶催化苯连菌素与三乙酰素乙酰化反应的动力学模型

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-08 DOI: 10.1016/j.bej.2025.109823

Stela Schiochett Virmond Vieira , Fernando Augusto Pedersen Voll , Nadia Krieger , David Alexander Mitchell

{"title":"A kinetic model for the solvent-free lipase-catalyzed acetylation of phlorizin with triacetin","authors":"Stela Schiochett Virmond Vieira , Fernando Augusto Pedersen Voll , Nadia Krieger , David Alexander Mitchell","doi":"10.1016/j.bej.2025.109823","DOIUrl":"10.1016/j.bej.2025.109823","url":null,"abstract":"<div><div>Little attention has been given to the kinetics of enzyme-catalyzed modification of flavonoids and flavonoid glycosides, especially the successive addition of multiple functional groups. The only currently available model is not consistent with the principles of enzyme kinetics. We developed a simple kinetic model, using the lipase-catalyzed acetylation of phlorizin in a solvent-free system as a case study. Our model assumes that the acetylation is irreversible, the concentration of the solvent/acetyl-donor remains constant and the lipase inactivates in a first-order process. We estimated the parameters of the model by fitting it to literature data for the lipase-catalyzed acetylation of phlorizin, with triacetin as the acetyl-donor. Our model fitted well to profiles for phlorizin, phlorizin-6”-<em>O</em>-acetate and phlorizin-3”,6”-<em>O</em>-diacetate obtained at several initial phlorizin concentrations. The estimated parameters were a specificity constant of 0.380 h<sup>−1</sup>%(w/v)<sup>−1</sup> for phlorizin, a selectivity of 0.362 for the second acetylation in relation to the first, saturation constants of 22.3 mmol L<sup>−1</sup> for phlorizin and 76.5 mmol L<sup>−1</sup> for phlorizin-6”-<em>O</em>-acetate, an inhibition constant for phlorizin-3”,6”-<em>O</em>-diacetate of 3.5 mmol L<sup>−1</sup> and a first-order denaturation constant of 0.0125 h<sup>−1</sup>. Our work not only presents a model that describes well the acetylation of phlorizin, it also demonstrates a strategy for modelling and parameter estimation that can be applied to other enzyme-catalyzed modifications of flavonoids and flavonoid glycosides.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109823"},"PeriodicalIF":3.7,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated strategy for efficient production and direct purification of EGFP from unclarified feedstocks 从未澄清的原料中高效生产和直接纯化EGFP的综合策略

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-07 DOI: 10.1016/j.bej.2025.109822

Jyun-Liang Liu , Shih-Pang Lin , The Duc Hanh Nguyen , Chanin Panjapornpon , Paweena Prapainainar , Kulpavee Jitapunkul , Chen‑Yaw Chiu , Bing-Lan Liu , Kuei-Hsiang Chen , Yu-Kaung Chang

{"title":"Integrated strategy for efficient production and direct purification of EGFP from unclarified feedstocks","authors":"Jyun-Liang Liu , Shih-Pang Lin , The Duc Hanh Nguyen , Chanin Panjapornpon , Paweena Prapainainar , Kulpavee Jitapunkul , Chen‑Yaw Chiu , Bing-Lan Liu , Kuei-Hsiang Chen , Yu-Kaung Chang","doi":"10.1016/j.bej.2025.109822","DOIUrl":"10.1016/j.bej.2025.109822","url":null,"abstract":"<div><div>Efficient recovery of recombinant proteins from unclarified feedstocks remains a major challenge in bioprocessing. Enhanced green fluorescent protein (EGFP) is widely used as a model protein, but its purification typically involves multiple clarification steps, increasing processing time and cost. This study presents an integrated strategy to optimize both upstream cultivation and downstream purification for EGFP production and recovery. A two-level, three-factor fractional factorial design (FFD) was employed to optimize environmental variables, identifying optimal conditions at pH 7.0, 26 °C, and 1.75 h of induction time. The optimized culture was scaled up in a 5 L bioreactor, yielding 3.66 mg/mL of EGFP and 36.86 mg/mL of total protein from 50 % (w/v) disrupted <em>E. coli</em> cells. Stirred fluidized bed adsorption (SFBA) using STREAMLINE DEAE adsorbent enabled direct purification from unclarified feedstock. At a linear velocity of 100 cm/h and a stirring speed of 100 rpm, the SFBA process achieved 96.3 % recovery, a 2.9-fold purification factor, and a productivity of 2.82 × 10<sup>−2</sup> mg/mL/min—surpassing previously reported methods. These results demonstrate an efficient, scalable purification approach for EGFP recovery directly from crude lysates, eliminating the need for centrifugation or filtration.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109822"},"PeriodicalIF":3.7,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The improved sparger enhances bioleaching of arsenic-bearing gold concentrate in a stirred tank 改进的喷淋器提高了含砷金精矿在搅拌槽中的生物浸出效果

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-05 DOI: 10.1016/j.bej.2025.109811

Zheng Lei , Xin Jiayu , He Changliu , Zhang Aijun , Tao Ranting , Zhang Xu

{"title":"The improved sparger enhances bioleaching of arsenic-bearing gold concentrate in a stirred tank","authors":"Zheng Lei , Xin Jiayu , He Changliu , Zhang Aijun , Tao Ranting , Zhang Xu","doi":"10.1016/j.bej.2025.109811","DOIUrl":"10.1016/j.bej.2025.109811","url":null,"abstract":"<div><div>Bioleaching is frequently applied to low - grade ores, and mixed - strain leaching can be influenced by factors such as temperature and substrates. This study compared the cooperative leaching effects of mesophilic and thermophilic strains, including <em>Leptospirillum ferrooxidans</em> (<em>L. f</em>), <em>Acidithiobacillus caldus</em> (<em>A. c</em>), and <em>Sulfobacillus thermosulfidooxidans</em> (<em>S. t</em>). An efficient strain combination of (<em>L. f</em> + <em>A. c</em>): <em>S. t</em> = 1:1 was identified. Subsequently, the original sparger was replaced with a nano - aeration pipe (NAP) to investigate its impact. Results indicated that NAP considerably elevated the oxygen uptake rate (OUR) and volumetric mass transfer coefficient (kLa) within the reaction system. Moreover, by analyzing the extracellular polymeric substances (EPS) content in leaching samples and assessing the microbial community, it was shown that the improved sparger, namely NAP, increased EPS content and altered the dominant bacterial groups in bioleaching. Leaching efficiency rose by 3.61 %, while daily energy consumption fell by 1.59 %. Scanning electron microscopy also confirmed that NAP enhanced microbial leaching. This study not only improves leaching efficiency and demonstrates economic potential but also offers a novel perspective for optimizing stirred - tank technology in bioleaching.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109811"},"PeriodicalIF":3.7,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling and simulation of biological CO conversion in trickle bed reactor and comparison with CSTR 滴流床生物CO转化的建模与仿真及与CSTR的比较

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-05 DOI: 10.1016/j.bej.2025.109819

Sambit Dutta, Marianna Krikeli, Hariklia N. Gavala, Ioannis V. Skiadas

{"title":"Modeling and simulation of biological CO conversion in trickle bed reactor and comparison with CSTR","authors":"Sambit Dutta, Marianna Krikeli, Hariklia N. Gavala, Ioannis V. Skiadas","doi":"10.1016/j.bej.2025.109819","DOIUrl":"10.1016/j.bej.2025.109819","url":null,"abstract":"<div><div>Syngas biomethanation in a trickle bed reactor (TBR) by mixed microbial consortia has reached a technology readiness level of 4–5. Scaling up the TBR requires investigating the operating conditions under which mass transfer or growth kinetics becomes the rate-limiting step. The novelty of the current work is the first-of-its-kind model development that can simulate microbial growth and biofilm formation together with the hydraulic and mass transfer behavior of TBR. The focus is on carboxydotrophic hydrogenogens, which biologically convert CO to CO<sub>2</sub> and H<sub>2</sub>, reducing gas phase CO levels. Model validation showed excellent predictive capacity for TBR of different volumes and height-to-diameter ratios. CO conversion efficiency was compared between 5000 ml (packed bed) semi-pilot-scale TBR, 220 ml (packed bed) lab-scale TBR, and 5000 ml (liquid volume) CSTR. Considering suspended growth, the semi-pilot-scale TBR outperformed the lab-scale TBR and CSTR due to a higher volumetric mass transfer coefficient. When biofilm growth was considered for the TBRs, CO conversion efficiency increased 1.03 times and 4 times for the semi-pilot- and lab-scale TBRs, respectively. Notably, the lab-scale TBR, with biofilm growth, surpassed the 22 times higher working volume CSTR. CO conversion efficiency in the lab-scale TBR increased from 23.3 % (suspended growth) to 86.3 % (biofilm growth) due to an increase of microbial cell mass from 0.081 to 0.29 g cells. Finally, simulations at varying liquid recirculation rates and constant gas flow rates for the TBR revealed a threshold at a liquid Reynolds number 35, where mass transfer limitations shift to microbial growth limitations.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109819"},"PeriodicalIF":3.7,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison and mechanism of CO2 sequestration by different carbonic anhydrase producing bacteria 不同产碳酸酐菌固存CO2的比较及机理

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-02 DOI: 10.1016/j.bej.2025.109812

Yaya Yuan , Chunxiang Qian

{"title":"Comparison and mechanism of CO2 sequestration by different carbonic anhydrase producing bacteria","authors":"Yaya Yuan , Chunxiang Qian","doi":"10.1016/j.bej.2025.109812","DOIUrl":"10.1016/j.bej.2025.109812","url":null,"abstract":"<div><div>With the rapid emission of CO<sub>2</sub> accelerating global warming, CO<sub>2</sub> geological storage has attracted wide attention due to its huge storage site and safe long-term storage capacity. Carbonic anhydrase (CA) can accelerate CO<sub>2</sub> hydration reaction and promote calcium carbonate precipitation. However, CA has poor thermal stability and high purification cost, so it is necessary to directly use CA producing bacteria for CO<sub>2</sub> geological storage under high temperature environment. In this paper, CA-producing bacterial strains resistant to medium and high temperature was screened from geological saline aquifers and compared with <em>Bacillus mucilaginosus</em> to reveal the reason for its resistance to medium and high temperature. Subsequently, the CO<sub>2</sub> dissolution sequestration capabilities of the two strains were explored at different temperatures. At 50℃, the CO<sub>2</sub> sequestration capacity of <em>Bacillus licheniformis</em> S5 was the highest, and reached 127 mM after 10 days of reaction, which was 26.8 % and 24.9 % higher than that of <em>B. licheniformis</em> CICC 10101 and <em>B. mucilaginosus</em>. Ultimately, the CO<sub>2</sub> sequestration mechanism of different CA-producing bacteria was analyzed. Under high temperature conditions, the enzyme activity of the unit-living bacteria of <em>B. licheniformis</em> S5 was higher, which was more conducive to the conversion of CO<sub>2</sub> into ionic state. This study provides theoretical support for CO<sub>2</sub> sequestration in some medium and high temperature scenarios.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109812"},"PeriodicalIF":3.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144204819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into magnetic biofilm carriers driving stable partial nitrification by selectively enriching ammonia-oxidizing bacteria 磁性生物膜载体通过选择性富集氨氧化细菌驱动稳定的部分硝化

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-29 DOI: 10.1016/j.bej.2025.109810

Fugeng Gong , Ao Xu , Dawen Gao , Xiaofei Gong , Chuchu Wang , Zheng Huang , Hong Liang

{"title":"Insights into magnetic biofilm carriers driving stable partial nitrification by selectively enriching ammonia-oxidizing bacteria","authors":"Fugeng Gong , Ao Xu , Dawen Gao , Xiaofei Gong , Chuchu Wang , Zheng Huang , Hong Liang","doi":"10.1016/j.bej.2025.109810","DOIUrl":"10.1016/j.bej.2025.109810","url":null,"abstract":"<div><div>Biofilm carriers can effectively support the retention of functional microorganisms augmenting the partial nitrification (PN) process. However, conventional biofilm carriers are ineffective in selectively enriching key ammonia-oxidizing bacteria (AOB) hindering the sustained nitrite (NO<sub>2</sub><sup>-</sup>-N) accumulation. This study introduces magnetic modified polyurethane (MF) carrier facilitates the selective enrichment of AOB and suppression of NO<sub>2</sub><sup>-</sup>-N oxidizing bacteria. The results demonstrate that the MF system achieves over 90 % NO<sub>2</sub><sup>-</sup>-N accumulation. The magnetic field fosters continuous biomass accumulation, while the regulated secretion of extracellular polymeric substances ensures the stability of AOB under fluctuating conditions. Microbial community analysis revealed that MF significantly enhanced biofilm formation potential and the resilience of system. Furthermore, compared to the control, the relative abundance of <em>Nitrosomonas</em> increased by 10 % in MF. These findings suggest that magnetic carriers represent an efficient strategy for enhancing AOB and offering a robust approach for the rapid initiation the PN process.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109810"},"PeriodicalIF":3.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144170413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0