Biochemical Engineering Journal最新文献

Vitamin supplementation for enhanced production of therapeutic nanobody in E.coli 补充维生素促进大肠杆菌治疗性纳米体的产生

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-29 DOI: 10.1016/j.bej.2025.109887

Richa Katiyar , Neha Gindi , Ishika Mittal , Claire Komives , Anurag S. Rathore

{"title":"Vitamin supplementation for enhanced production of therapeutic nanobody in E.coli","authors":"Richa Katiyar , Neha Gindi , Ishika Mittal , Claire Komives , Anurag S. Rathore","doi":"10.1016/j.bej.2025.109887","DOIUrl":"10.1016/j.bej.2025.109887","url":null,"abstract":"<div><div>As low yields of nanobodies in <em>E.coli.</em> periplasm is a major concern for their large-scale production, this study explores the use of vitamins for enhanced production of a recombinant periplasmic nanobody product. Nanobody producing <em>E.coli</em> cells were cultivated in the modified Terrific-Broth supplemented with three different vitamins (biotin denoted as B, cyanocobalamin as C, and D-calcium pantothenate as P). Multiple combinations (B+P, B+C, P + C, and B+P + C) were examined, with B+P offering the highest specific growth rate, total biomass productivity, product accumulation and product expression. Densitometry analysis revealed 129.17 ± 1.1 mg/L nanobodies in case of B+P supplementation (24 % higher than control). B+P supplementation also offered 21 % better fermentation yield than control (as per RP-HPLC). The yield of purified nanobody product in case of B+P supplementation was at 35.0 ± 2.1 mg/L with purity levels of ≥ 80 %. Intact mass and peptide mapping analysis of purified nanobody revealed molecular weight of 15.2 kDa and 91 % sequence coverage with the theoretical nanobody sequence respectively. Spent media analysis revealed production of low acetate in case of B+P supplementation of media when compared to other conditions examined. Hence, the B+P supplementation could be used to produce improved amount of periplasmic nanobody in future.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109887"},"PeriodicalIF":3.7,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144739424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Life cycle assessment of anaerobic digestion system under ammonia inhibition: Evaluation of exogenous media addition 氨抑制下厌氧消化系统的生命周期评价：外源培养基添加的评价

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-28 DOI: 10.1016/j.bej.2025.109886

Meiying Ye , Shuo Gao , Panpan Wang , Yufang Wei , Jiaqi Hou , Mingxiao Li , Yanping Liu , Ziyi Yang

{"title":"Life cycle assessment of anaerobic digestion system under ammonia inhibition: Evaluation of exogenous media addition","authors":"Meiying Ye , Shuo Gao , Panpan Wang , Yufang Wei , Jiaqi Hou , Mingxiao Li , Yanping Liu , Ziyi Yang","doi":"10.1016/j.bej.2025.109886","DOIUrl":"10.1016/j.bej.2025.109886","url":null,"abstract":"<div><div>Exogenous media addition in the ammonia-inhibited anaerobic digestion (AD) is considered as an efficient approach. Comprehensively evaluation of the environmental impact of adding exogenous media can provide the support on application. Under ammonia inhibition, life cycle assessment (LCA) was used to compare the AD system (Scenario 1) and the AD system with the addition of magnetite powder and polyurethane filler (Scenario 2). In the midpoint environmental impact assessment, among the 18 categories of environmental impacts, Scenario 2 performed the weak negative impact on the environment compared to the non-added scenario. Ionizing radiation and freshwater eutrophication in Scenario 2 were less than 50 % of those in Scenario 1. In the endpoint environmental impact assessment, the negative impacts on human health, ecosystems, and resource of Scenario 2 were lower than 82 % of those in the non-additive scenario. Monte Carlo simulation was used to carry out uncertain analysis and calculation, which effectively reduced the deviation brought by inventory analysis and improved the reliability of the analysis. The sensitivity analysis results showed that power input and power sources significantly affect environmental impacts. Reducing power input and using renewable or clean energy could decrease pollution levels. The scenario of adding exogenous media showed good environmental benefits, and subsequent optimization could focus on the aspects of power source (such as using green electricity) and power efficiency.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109886"},"PeriodicalIF":3.7,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144714482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of C/N ratio and sulfate concentration on simultaneous partial nitrification and denitrifying phosphorus removal (SPNDPR) process C/N和硫酸盐浓度对同时部分硝化和反硝化除磷（SPNDPR）过程的影响

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-25 DOI: 10.1016/j.bej.2025.109885

Hui Jin , Tianqi Zhang , Yihan Cao , Jie Wang , Pengfei Shan , Chengchao Xin , Xiaoling Zhang , Aixia Chen , Wenjuan Yang

{"title":"Effects of C/N ratio and sulfate concentration on simultaneous partial nitrification and denitrifying phosphorus removal (SPNDPR) process","authors":"Hui Jin , Tianqi Zhang , Yihan Cao , Jie Wang , Pengfei Shan , Chengchao Xin , Xiaoling Zhang , Aixia Chen , Wenjuan Yang","doi":"10.1016/j.bej.2025.109885","DOIUrl":"10.1016/j.bej.2025.109885","url":null,"abstract":"<div><div>Simultaneous partial nitrification and denitrifying phosphorus removal (SPNDPR) is a promising strategy for treating low carbon-to-nitrogen (C/N) ratio wastewater. This study explored the establishment, performance, and microbial mechanisms of the SPNDPR process in a sequencing batch reactor (SBR) under varying C/N ratios (6.67–3.33) and sulfate concentrations (50–300 mg/L). Operated under low dissolved oxygen (DO) and intermittent aeration, the system achieved high removal efficiencies for ammonium nitrogen (NH₄⁺-N, 99.88 ± 0.55 %), total inorganic nitrogen (TIN, 79.90 ± 7.17 %), PO₄³ ⁻-P (91.67 ± 3.72 %), and chemical oxygen demand (COD, 92.60 ± 0.27 %). The ratio of ammonia-oxidizing bacteria (AOB, Nitrosomonas) to nitrite-oxidizing bacteria (NOB, Nitrospira) increased from 0.34 to 2.09, indicating successful partial nitrification. Polyphosphate-accumulating organisms (PAOs, <em>Candidatus Accumulibacter</em>) and glycogen-accumulating organisms (GAOs, <em>Candidatus Competibacter</em>) were effectively enriched, facilitating phosphorus removal and denitrifying activity. At a C/N ratio of 5, nutrient removal remained stable; however, further reduction to 3.33 led to decreased TIN (83.99 ± 6.34 %) and PO₄<sup>3-</sup>-P (88.08 ± 4.37 %) removal. Increasing influent sulfate concentrations adversely affected performance, with removal efficiencies of TIN and PO<sub>4</sub><sup>3-</sup>-P declining to 87.22 ± 3.57 % and 60.22 ± 9.47 %, respectively, at 300 mg/L. High sulfate levels also altered the microbial community, with sulfate-reducing bacteria (SRB), <em>Desulfomicrobium</em> (1.81 %) and <em>Desulfosporosinus</em> (5.01 %), becoming prevalent.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109885"},"PeriodicalIF":3.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144720913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep multi-kernel cell clustering for single-cell RNA sequencing data 单细胞RNA测序数据的深度多核细胞聚类

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-25 DOI: 10.1016/j.bej.2025.109877

Maoxuan Yao , Lina Ren

{"title":"Deep multi-kernel cell clustering for single-cell RNA sequencing data","authors":"Maoxuan Yao , Lina Ren","doi":"10.1016/j.bej.2025.109877","DOIUrl":"10.1016/j.bej.2025.109877","url":null,"abstract":"<div><div>Although existing deep learning methods for single-cell RNA sequencing (scRNA-seq) data can handle high-dimensional data and extract complex features, they also have the issue that the learned representations do not consider the clustering structure, leading to difficulties in linear separation. To address this, we propose a deep multi-kernel cell clustering network for scRNA-seq data, termed scDMKC, to cluster scRNA-seq data by simultaneously optimizing the multi-kernel representation of scRNA-seq data and the cell partitioning, enhancing the cell clustering performance by leveraging the strengths of multi-kernel learning and deep learning in capturing linearly separable data structures. To effectively learn the multi-kernel representation of scRNA-seq data, we propose a multi-kernel representation learner that adaptively selects an appropriate combination of multiple kernels to map the hidden representations of the data into the kernel space, thereby capturing the underlying linearly separable structure of the scRNA-seq data. In order to jointly optimize the multi-kernel representation of scRNA-seq data and the cell partitioning, a ZINB-based self-supervised strategy is developed. This strategy not only enhances the linear separability of the learned kernel representations but also improves clustering performance. Extensive experiments on various real scRNA-seq datasets were conducted. The experimental results indicate that our proposed scDMKC model significantly outperforms most of the existing cell clustering methods.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109877"},"PeriodicalIF":3.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144739213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive carboxylated PAN nanofiber membranes from expired egg white waste for antibacterial applications 用过期蛋清制备生物活性羧化聚丙烯腈纳米纤维膜用于抗菌

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-24 DOI: 10.1016/j.bej.2025.109882

Edouard Gnoumou , Thi Tam An Tran , Quang-Vinh Le , Nguyen The Duc Hanh , Nanthiya Hansupalak , Bing-Lan Liu , Chen-Yaw Chiu , Chi-Yun Wang , Kuei-Hsiang Chen , Yu-Kaung Chang

{"title":"Bioactive carboxylated PAN nanofiber membranes from expired egg white waste for antibacterial applications","authors":"Edouard Gnoumou , Thi Tam An Tran , Quang-Vinh Le , Nguyen The Duc Hanh , Nanthiya Hansupalak , Bing-Lan Liu , Chen-Yaw Chiu , Chi-Yun Wang , Kuei-Hsiang Chen , Yu-Kaung Chang","doi":"10.1016/j.bej.2025.109882","DOIUrl":"10.1016/j.bej.2025.109882","url":null,"abstract":"<div><div>The rapid expansion of the egg industry has led to the accumulation of expired egg white waste, much of which remains underutilized and contributes to environmental pollution. Given the natural antimicrobial proteins in chicken egg white (CEW), such as lysozyme (LYZ) and ovotransferrin, repurposing expired CEW offers a sustainable strategy to reduce waste while addressing concerns about antibiotic resistance. In this study, polyacrylonitrile (PAN) nanofiber membranes were functionalized with −COOH groups via alkaline hydrolysis to facilitate protein attachment. Expired CEW was immobilized onto the modified membranes through weak ionic and covalent interactions, forming P-COOH-EWP (egg white proteins) nanofiber membranes. The membranes were characterized for surface morphology, chemical composition, and antibacterial activity against <em>Escherichia coli</em> and <em>Staphylococcus aureus</em>. Additional evaluations included enzymatic activity, cytotoxicity, reusability, and storage stability. The results confirmed successful immobilization of EWP. The P-COOH-EWP membranes demonstrated vigorous antibacterial activity, with 86.57 % efficacy against <em>E. coli</em> and 75.05 % against <em>S. aureus</em>, and showed excellent biocompatibility without cytotoxic effects. They retained more than 72 % antibacterial activity after four weeks of storage at room temperature. However, activity decreased by 40 % after two uses, indicating suitability for single-use applications. This work presents a cost-effective and environmentally friendly approach to valorizing expired CEW waste into functional antibacterial nanomaterials for biomedical, food packaging, and environmental applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109882"},"PeriodicalIF":3.7,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144714481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green extraction and purification of phycocyanin from Spirulina platensis: Evaluation of its bioactivity and photoprotection 螺旋藻藻蓝蛋白的绿色提取纯化及其生物活性和光防护性评价

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-23 DOI: 10.1016/j.bej.2025.109881

Zahrotul Firdaus, Safrina Dyah Hardiningtyas , Iriani Setyaningsih

{"title":"Green extraction and purification of phycocyanin from Spirulina platensis: Evaluation of its bioactivity and photoprotection","authors":"Zahrotul Firdaus, Safrina Dyah Hardiningtyas , Iriani Setyaningsih","doi":"10.1016/j.bej.2025.109881","DOIUrl":"10.1016/j.bej.2025.109881","url":null,"abstract":"<div><div>Phycocyanin extracted from the microalga <em>Spirulina platensis</em> demonstrates diverse biological activities, with its effectiveness depending on its purity. This study focused on developing an eco-friendly extraction method by combining ultrasonication and freeze–thawing, establishing a purification approach using a combination of an aqueous two-phase system with a natural deep eutectic solvent (ATPS-NaDES) alongside precipitation techniques, and assessing phycocyanin’s antioxidant, UV-photoprotective, and antimicrobial properties relative to its purity. The findings revealed that the highest purity index (PI) of phycocyanin (2.49 ± 0.03), achieving cosmeceutical-grade quality with a recovery rate of 69.42 %, was obtained using the combined ATPS-NaDES and precipitation method (N + P). Phycocyanin demonstrated strong antioxidant activity, with IC₅₀ values of 38.15 ± 0.56 ppm for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 25.70 ± 0.25 ppm for ABTS, along with a Trolox equivalent value of 204.33 μg/mL at 75 ppm in the ferric reducing antioxidant power (FRAP) assay. Its UV-photoprotective effect was notable, showing a sun protection factor (SPF) of 40.23 ± 0.01 at 5 mg/mL. Additionally, phycocyanin exhibited effective antimicrobial activity against <em>Staphylococcus aureus</em>, <em>Escherichia coli</em>, and <em>Cutibacterium acnes</em>. This study highlights phycocyanin's multifunctional bioactivities, underscoring its potential as a natural antioxidant, photoprotective, and antimicrobial ingredient for cosmetic applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109881"},"PeriodicalIF":3.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144714479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic filtration device for high throughput process development 用于高通量工艺开发的微流控过滤装置

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-23 DOI: 10.1016/j.bej.2025.109880

Nusrat Jahan , Jaanvi Gandhi , Nicolas Szita , Sheng-Ching Wang , Spyridon Konstantinidis , Marco PC Marques

{"title":"Microfluidic filtration device for high throughput process development","authors":"Nusrat Jahan , Jaanvi Gandhi , Nicolas Szita , Sheng-Ching Wang , Spyridon Konstantinidis , Marco PC Marques","doi":"10.1016/j.bej.2025.109880","DOIUrl":"10.1016/j.bej.2025.109880","url":null,"abstract":"<div><div>Sustainable biopharmaceutical manufacturing requires cost-effective, scalable, and efficient processes. To achieve this goal, access to scalable screening platforms providing rapid and high-quality data with low material requirements is required. While cell culture/fermentation and purification technologies meeting these criteria have been developed and deployed, microscale filtration solutions, which enable screening a wide range of filtration products and conditions, are largely absent. Microfluidic filtration devices are uniquely positioned to fill this technology gap since they can provide the necessary throughput for screening applications with low feed volumes and in-line monitoring. However, a lack of standardized approaches limits their industrial adoption. We present a microfluidic tangential flow filtration (μTFF) device that can accommodate any flat sheet-membrane. Membrane exchange is straightforward, enabling application in single-use or re-use formats. We successfully tested a wide flux range (30–1055 litres per square meter per hour, LMH), observed recovery yields exceeding 80 % in single-pass mode (1 bar transmembrane pressure, TMP), and demonstrated cleaning in place (CIP) procedures for extended membrane use with multiple filtration cycles (recoveries > 80 %). Furthermore, we integrated sensors to facilitate automation and generation of scale-relevant data, and provided criteria to facilitate pump selection. Our μTFF device offers, therefore, a standardised design paving the way for off-the-shelf microfluidic solutions for filtration optimisation, thus enhancing efficiency and effectiveness of bioprocess development.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109880"},"PeriodicalIF":3.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144723876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adsorption of Pb2+ and Cd2+ on silica-modified and nitrogen self-doped lotus leaf biochar: Adsorption behavior and mechanism 二氧化硅修饰和氮自掺杂荷叶生物炭对Pb2+和Cd2+的吸附：吸附行为和机理

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-19 DOI: 10.1016/j.bej.2025.109873

Yuanping Li , Suzhen Hou , Yaoning Chen , Yihuan Liu , Mengyang Zhao , Hongjuan Jiang , Nianping Chi , Guowen He , Shunyao jia

{"title":"Adsorption of Pb2+ and Cd2+ on silica-modified and nitrogen self-doped lotus leaf biochar: Adsorption behavior and mechanism","authors":"Yuanping Li , Suzhen Hou , Yaoning Chen , Yihuan Liu , Mengyang Zhao , Hongjuan Jiang , Nianping Chi , Guowen He , Shunyao jia","doi":"10.1016/j.bej.2025.109873","DOIUrl":"10.1016/j.bej.2025.109873","url":null,"abstract":"<div><div>Biochar and its modified products have received much attention in the adsorption treatment of heavy metal wastes, but there has been great potential for improvement in efficiency and cost. In this study, nitrogen-containing lotus leaves taken from lotus root harvests were pyrolyzed and modified by the silicon from industrial by-product micro-silicon powder, and then the Si-modified and nitrogen self-doped lotus leaf biochar (Si@N-BC) was prepared as an efficient material for the adsorption of Pb<sup>2+</sup> and Cd<sup>2+</sup> in aqueous solution. The results demonstrated that the adsorption performance of Si@N-BC was significantly enhanced than the pristine biochar, and the maximum adsorption capacities of Pb<sup>2+</sup> and Cd<sup>2+</sup> were estimated based on the Langmuir model to be 363.60 mg/g and 56.04 mg/g, respectively. The adsorption kinetic experiment indicated that the adsorption reaction was dominated by chemisorption. Moreover, the high performance of Si@N-BC is not only related to ion exchange and surface precipitation, but also the complexation reactions between oxygen-containing functional groups, nitrogen-containing functional groups, and silicon-containing functional groups. In conclusion, Si@N-BC is a novel and promising adsorbent that is conducive to the resourceful utilization of lotus leaf and micro-silicon powder, and its multiple functional groups can work together to achieve efficient removal of heavy metals from water.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109873"},"PeriodicalIF":3.7,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144694994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Species classification of microalgae using a CNN-based deep learning approach under optimal cultivation conditions 最优培养条件下基于cnn深度学习的微藻种类分类

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-19 DOI: 10.1016/j.bej.2025.109879

Gokce Kendirlioglu Simsek , Merve Ertargin , Laura Pezzolesi , Rossella Pistocchi , Ozal Yildirim , A. Kadri Cetin

{"title":"Species classification of microalgae using a CNN-based deep learning approach under optimal cultivation conditions","authors":"Gokce Kendirlioglu Simsek , Merve Ertargin , Laura Pezzolesi , Rossella Pistocchi , Ozal Yildirim , A. Kadri Cetin","doi":"10.1016/j.bej.2025.109879","DOIUrl":"10.1016/j.bej.2025.109879","url":null,"abstract":"<div><div>Microalgae possess significant potential in a wide range of applications due to their valuable bioactive compounds. They are utilized in biofuel production to reduce dependence on fossil fuels, in wastewater treatment for the biological removal of heavy metals and pollutants, in carbon capture for mitigating climate change, and in the pharmaceutical and nutraceutical industries as supplements. As their applications expand, the accurate and efficient identification of microalgal species becomes increasingly important. Traditional classification methods are time-consuming and rely heavily on expert knowledge. The main aim of this study is to develop a reliable, fast, and expert-independent deep learning-based approach for the classification of microalgae species using microscopic images. In this context, deep learning techniques, specifically convolutional neural network (CNN)-based models were employed to classify microalgal species. Four widely used pre-trained CNN architectures (ResNet152, DenseNet201, MobileNetV2, and EfficientNetB0), along with a custom-designed CNN, were implemented. The models were trained and tested on a labeled dataset consisting of microscopic images of Chlorella vulgaris, Scenedesmus acutus, and Haematococcus pluvialis. The classification models achieved accuracy rates ranging from 96.87 % (Custom CNN) to 100 % (DenseNet201), demonstrating the potential of CNN-based approaches in automating and improving microalgae species identification.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109879"},"PeriodicalIF":3.7,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144694995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial domestication and response of low C/N ammonia nitrogen wastewater treated by zonal influent micro-pressure internal circulation reactor 分区进水微压内循环反应器处理低碳氮比氨氮废水的微生物驯化及响应

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-17 DOI: 10.1016/j.bej.2025.109878

Fan Wang, Fangyuan Zhang, Wenai Liu, Yuying Fan, Kailun Yang, Jinhao Pu, Dejun Bian

{"title":"Microbial domestication and response of low C/N ammonia nitrogen wastewater treated by zonal influent micro-pressure internal circulation reactor","authors":"Fan Wang, Fangyuan Zhang, Wenai Liu, Yuying Fan, Kailun Yang, Jinhao Pu, Dejun Bian","doi":"10.1016/j.bej.2025.109878","DOIUrl":"10.1016/j.bej.2025.109878","url":null,"abstract":"<div><div>In this study, by distributing the influent in a micro-pressure internal circulation reactor (MPR) at a flow rate ratio of 1:2:1 to the anaerobic zone, anoxic zone, and aerobic zone, efficient denitrification of low C/N ammonia nitrogen wastewater was successfully achieved without the need for additional carbon sources or nitrified liquid reflux. Compared with the traditional treatment method of adding carbon sources, the treatment cost was reduced. By regulating the dissolved oxygen (DO) content in the growth environment of microbial flora, the nitrifying flora was first enriched, followed by the denitrifying and simultaneous nitrification-denitrification (SND) bacteria flora. During this process, the diversity of microbial populations decreased with the increase of DO concentration. The removal efficiency of COD, NH<sub>4</sub><sup>+</sup> -N and TN reached 95 %, 85 % and 80 % respectively. The 16S rRNA amplicon sequencing showed that the increase of dissolved oxygen during domestication promoted the proliferation of nitrifying bacteria, the relative abundance of nitrifying genes increased, while the secretion of extracellular polymers increased, and the activities of dehydrogenase activity and specific aerobic rate increased. Then, with the decrease of DO concentration, the relative abundance of nitrifying bacteria at the genus level wasn′t affected. However, the denitrifying functional bacteria increased significantly, especially the abundance of bacteria with SND functions, and the TN removal amount in the aerobic zone reached 21.24 % of the total removal amount.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109878"},"PeriodicalIF":3.7,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0