Biochemical Engineering Journal最新文献

Developing novel photoactive bimetal Cr/Ag-codoped CeO2 nanozymes with synergistic activity toward biophotodegradation of organic dyes from real water media and textile wastewater 开发新型光活性双金属Cr/ ag共掺杂CeO2纳米酶，协同降解真实水介质和纺织废水中的有机染料

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-23 DOI: 10.1016/j.bej.2025.109765

Anjan Kumar , Ahmed M. Naglah , Yashwantsinh Jadeja , Suhas Ballal , Shaker Al-Hasnaawei , Abhayveer Singh , T. Krithiga , Subhashree Ray , Chou-Yi Hsu

{"title":"Developing novel photoactive bimetal Cr/Ag-codoped CeO2 nanozymes with synergistic activity toward biophotodegradation of organic dyes from real water media and textile wastewater","authors":"Anjan Kumar , Ahmed M. Naglah , Yashwantsinh Jadeja , Suhas Ballal , Shaker Al-Hasnaawei , Abhayveer Singh , T. Krithiga , Subhashree Ray , Chou-Yi Hsu","doi":"10.1016/j.bej.2025.109765","DOIUrl":"10.1016/j.bej.2025.109765","url":null,"abstract":"<div><div>A novel biophotodegradation method of organic dyes was developed using photoactive bimetal Cr/Ag-codoped CeO<sub>2</sub> nanozymes with synergistic activity toward dye biophotodegradation from real water media and textile wastewater. The photoactive bimetal Cr/Ag-codoped CeO<sub>2</sub> nanozymes were synthesized and then comprehensively identified using several characterization methods. The developed bimetal Cr/Ag-codoped CeO<sub>2</sub> nanozymes revealed enhanced peroxidase-like activity. The introduced bimetal Cr/Ag-codoped CeO<sub>2</sub> nanozymes exhibited 6.66-order, 3.53-fold, and 2.0-order higher enzyme-like activity than the CeO<sub>2,</sub> Ag-CeO<sub>2,</sub> and Cr-CeO<sub>2</sub>, respectively, revealing the synergistic effect of bimetal-codoping on their catalytic activity. The photonanozymatic activity of the nanozymes was found to be 1.47-fold and 2.48-order higher than their nanozymatic and photocatalytic activity, respectively. Hence, they were applied for environmental mineralization applications via biophotodegradation of methylene blue. The effective factors on the yield of biodegradation were optimized by the one-factor-at-a-time method, providing maximal biophotodegradation of 99.95 % within 7.0 min. The storage and cycling stability of the developed photoactive bimetal Cr/Ag-codoped CeO<sub>2</sub> nanozymes were assessed, exhibiting that they are sable for at-least 30 days and 10 recycles without any change in their activity. The method was then practically applied for the treatment of water media (biophotodegradation, 98.7–99.6 %) and textile wastewater (biophotodegradation, 89.6–99.5 %).</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109765"},"PeriodicalIF":3.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143868684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing an efficient open-pond microalgae cultivation system for producing biomass and fucoxanthin using Pavlova granifera IML2238 利用花叶巴甫洛夫（Pavlova granifera） IML2238开发生产生物质和岩藻黄素的高效开放池塘微藻培养系统

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-22 DOI: 10.1016/j.bej.2025.109762

Chun-Yen Chen , Yu-Han Chang , Yoong Kit Leong , Jo-Shu Chang

{"title":"Developing an efficient open-pond microalgae cultivation system for producing biomass and fucoxanthin using Pavlova granifera IML2238","authors":"Chun-Yen Chen , Yu-Han Chang , Yoong Kit Leong , Jo-Shu Chang","doi":"10.1016/j.bej.2025.109762","DOIUrl":"10.1016/j.bej.2025.109762","url":null,"abstract":"<div><div>Fucoxanthin is a marine carotenoid recognized for its strong antioxidant and free radical scavenging properties. However, most research on microalgae-based fucoxanthin production has been limited to laboratory scales (typically under 200 liters), providing limited insights for commercial applications. Therefore, there is an urgent need to establish scaled-up fucoxanthin-producing systems. In this study, a 6-ton open-pond system was employed to produce fucoxanthin using a fucoxanthin-rich microalgal strain <em>Pavlova granifera</em> IML2238. With an inoculum size of 0.10 g/L, batch cultivation achieved biomass and fucoxanthin productivity of 74.9 and 3.24 mg/L/d, respectively. A semi-batch process, replacing 50 % of the medium volume in each cycle, resulted in biomass and fucoxanthin productivity of 80.2 and 4.31 mg/L/d, respectively. Repeated semi-batch replacement at 50 % maintained stable biomass and fucoxanthin productivity at 73.7 and 4.05 mg/L/d. The fucoxanthin produced demonstrated high antioxidant activity, with a total phenolic content of 29.8 mg GAE/g, confirming its potential for commercial use.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109762"},"PeriodicalIF":3.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extending the green stage of Haematococcus pluvialis as a crucial precursor for efficient astaxanthin production: Optimization via Taguchi design 延长雨红球菌绿色阶段作为高效虾青素生产的关键前体：田口设计优化

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-22 DOI: 10.1016/j.bej.2025.109763

Christina Samara, Alexandros Biziouras, Georgia Papapanagiotou, Christos Chatzidoukas

{"title":"Extending the green stage of Haematococcus pluvialis as a crucial precursor for efficient astaxanthin production: Optimization via Taguchi design","authors":"Christina Samara, Alexandros Biziouras, Georgia Papapanagiotou, Christos Chatzidoukas","doi":"10.1016/j.bej.2025.109763","DOIUrl":"10.1016/j.bej.2025.109763","url":null,"abstract":"<div><div>The microalga <em>Haematococcus pluvialis</em> can accumulate the natural antioxidant astaxanthin up to 5 % of its dry weight under multi-stress conditions. Increasing this percentage remains challenging; therefore, the key to efficient astaxanthin production lies significantly on the formation of a vigorous, high-density, and synchronized green-cell population prior to the induction of carotogenesis. Despite its importance, optimization of the vegetative growth phase is often underestimated. This study employs an L9-Taguchi design of experiments to systematically investigate the combinatorial effects of four key cultivation factors - temperature, sodium bicarbonate, nitrogen, and phosphorus - on the vegetative growth of <em>H. pluvialis</em>. Temperature emerges as the most influential factor for biomass production, showing an adverse effect with its increment. Nitrogen and phosphorus limitation combined with sodium bicarbonate supplementation induces a premature shift to the non-proliferative red stage. Overall, the optimal conditions identified for vegetative stage maintenance and biomass maximization are: 22 °C, 250 mg L<sup>−1</sup> sodium bicarbonate, 150 mg L<sup>−1</sup> nitrogen and 40 mg L<sup>−1</sup> phosphorus. These conditions result in the production of 1.53 g L<sup>−1</sup> green dry biomass after 15 days of cultivation, outperforming by 0.3 g L<sup>−1</sup> suboptimal green phase conditions. Post-verification of the Taguchi-derived predictions is experimentally demonstrated paving the way for the formulation of dense and robust green-cell populations that will be concomitantly transferred to the red stage.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109763"},"PeriodicalIF":3.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143868685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feeding regime selectively enriching acetoclastic methanogens to enhance energy production in anaerobic digestion systems 饲喂方式选择性地富集丙酮发酵产甲烷菌，以提高厌氧消化系统的能量生产

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-21 DOI: 10.1016/j.bej.2025.109764

Huanhuan Chang , Qidong Yin , Kai He , Jo De Vrieze , Guangxue Wu

{"title":"Feeding regime selectively enriching acetoclastic methanogens to enhance energy production in anaerobic digestion systems","authors":"Huanhuan Chang , Qidong Yin , Kai He , Jo De Vrieze , Guangxue Wu","doi":"10.1016/j.bej.2025.109764","DOIUrl":"10.1016/j.bej.2025.109764","url":null,"abstract":"<div><div>Applying the r/K selection theory to explore the distribution patterns of acetoclastic methanogens under varying conditions is rare, although it can offer a basis for optimizing community structure and enhancing methane production in anaerobic digestion systems. Different operating modes (sequencing batch reactors, SBRs; and continuous-flow reactors, CFRs) and solids retention times (SRTs; 15 and 50 days) were adopted to acclimate different acetoclastic methanogens in acetate-fed anaerobic reactors. SBRs exhibited a significantly higher CH<sub>4</sub> production rate than CFRs (<em>P</em> = 0.037). <em>Methanosarcina</em> exhibited a higher relative abundance in SBRs (13.7 ∼ 16.1 %) than in CFRs (0.2 ∼ 0.3 %), aligning with its typical r-strategist characteristics. <em>Methanothrix</em> showed a higher enrichment in CFRs (33.1 ∼ 39.6 %) compared to SBRs (26.8 ∼ 29.9 %) at the same SRT, indicating K-strategist behavior. The SBRs had the potential to co-enrich both types of methanogens. Feeding regimes played a more pivotal role in the distribution of methanogens than SRT. The dominant bacteria, such as <em>Desulfococcus</em> and <em>Mesotoga</em>, as well as the archaeon <em>Methanothrix</em>, were auxotrophic in some essential amino acids, implying potential cross-feeding interactions. This study provides key insights into ecological strategies by linking microbiology with environmental technologies to enrich target methanogenic communities and enhance methane production.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109764"},"PeriodicalIF":3.7,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fermentation optimization and metabolomic analysis of ε-polylysine production in Streptomyces albulus 白色链霉菌ε-聚赖氨酸的发酵优化及代谢组学分析

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-18 DOI: 10.1016/j.bej.2025.109758

Yuanyuan Yu , Li Xia , Jianping Wen

引用次数: 0

Nitrogen removal performance and feeding strategy optimisation in a nitritation denitrification step-feed sequencing batch reactor system 硝化反硝化步进序批式反应器系统脱氮性能及进料策略优化

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-18 DOI: 10.1016/j.bej.2025.109761

Yihan Wang , Zhenjun Wu , Qingwen Qin , Lijie Chen , Ming Cai , Xin Chen , Yutong Liu , Ziang Su , Xinyi Fan , Lang Cheng

引用次数: 0

Whole-cell catalytic synthesis of cadaverine by recombinant Corynebacterium glutamicum using corncob residue as carbohydrate feedstock 重组谷氨棒状杆菌以玉米芯渣为原料全细胞催化合成尸胺

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-17 DOI: 10.1016/j.bej.2025.109760

Ying-Ying Xu, Bin Zhang, Jie Bao

引用次数: 0

The flow of proteins through the mammalian secretory pathway described by a phenomenological-based semi-physical model 用基于现象学的半物理模型描述了哺乳动物分泌途径中蛋白质的流动

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-17 DOI: 10.1016/j.bej.2025.109759

Santiago Benavides-López , Laura M. Muñoz-Echeverri , Mauricio A. Trujillo-Roldán , Norma A. Valdez-Cruz

引用次数: 0

Simulation of bioreactor flow environments for large-scale animal cell culture conditions relevant to cultivated meat production 与养殖肉类生产相关的大规模动物细胞培养条件的生物反应器流动环境模拟

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-11 DOI: 10.1016/j.bej.2025.109753

Kira Kiviat , David E. Block , Harishankar Manikantan

{"title":"Simulation of bioreactor flow environments for large-scale animal cell culture conditions relevant to cultivated meat production","authors":"Kira Kiviat , David E. Block , Harishankar Manikantan","doi":"10.1016/j.bej.2025.109753","DOIUrl":"10.1016/j.bej.2025.109753","url":null,"abstract":"<div><div>Cultivated meat has the potential to mitigate many detrimental effects of conventional meat production on land use and greenhouse gas emissions. However, to meet an increasing demand for sustainable protein sources and to achieve cost parity with conventionally grown meat, animal cells will likely be required to be produced in bioreactors at an order of magnitude larger scale than has been done so far. To help de-risk this scale up, simulations of plausible bioreactor configurations were performed at a series of scales ranging from 200 L to 200,000 L using computational fluid dynamics. Several different bubble drag models were compared, and the one that predicted the lowest mass transfer and highest shear was used in order to be conservative about the predicted cell environment. The distributions of shear stress, oxygen mass transfer coefficient, and Kolmogorov length scale were compared across the bioreactor scales, showing only minor changes with increasing scale. The case of a Rushton and pitched impeller was compared to a case with two Rushton impellers, and the latter was found to have higher mass transfer and only slightly higher shear for a given power input. This study provides a step towards predicting animal cell culture performance at the scales needed for sustainable cultivated meat production.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109753"},"PeriodicalIF":3.7,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid detection of Staphylococcus aureus in animal-derived foods using aptamer-based fluorescent lateral flow biosensors 基于适配体的荧光侧流生物传感器快速检测动物源性食品中的金黄色葡萄球菌

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-11 DOI: 10.1016/j.bej.2025.109756

Shuxian Zhou , Yinghui Wang , Haifang Wang , Shouyi Dou , Mengyue Liu , Haowei Dong , Zhengtao Li , Donghan Li , Jingjing Liu , Xia Sun , Yemin Guo , Ibrahim A. Darwish , Yanfang Wu

{"title":"Rapid detection of Staphylococcus aureus in animal-derived foods using aptamer-based fluorescent lateral flow biosensors","authors":"Shuxian Zhou , Yinghui Wang , Haifang Wang , Shouyi Dou , Mengyue Liu , Haowei Dong , Zhengtao Li , Donghan Li , Jingjing Liu , Xia Sun , Yemin Guo , Ibrahim A. Darwish , Yanfang Wu","doi":"10.1016/j.bej.2025.109756","DOIUrl":"10.1016/j.bej.2025.109756","url":null,"abstract":"<div><div><em>Staphylococcus aureus</em> (<em>S. aureus</em>), commonly found in animal-derived foods, poses a significant threat to food safety. Traditional detection methods often suffer from limited sensitivity and selectivity, are time-consuming, and involve complex procedures. In this study, fluorescent lateral flow biosensors (LFB) based on aptamer recognition were developed for the rapid and cost-effective detection of <em>S. aureus</em>. The method utilizes a Cy5-labeled aptamer (Cy5-Apt) as a signal recognition molecule. Detection is achieved through the competitive binding of <em>S. aureus</em> and a complementary strand to the aptamer, enabling the fluorescence intensity measurement on the LFB within 10 min. Key factors including the Cy5-Apt concentration, the length of the linker arm, the position and length of the complementary strand to the aptamer, BSA concentration, and buffer composition were optimized. The method showed good linear response for the quantitative detection of <em>S. aureus</em> in the concentration range of 2.8 × 10<sup>1</sup> to 2.8 × 10<sup>7</sup> CFU mL<sup>−1</sup>, and the limit of detection (LOD) was 1.65 CFU mL<sup>−1</sup>. Application tests in milk and chicken meat confirmed the selectivity and sensitivity of this enzyme- and antibody-free approach. This aptamer-based method offers a promising solution for low-cost, rapid, and efficient detection of <em>S. aureus</em> in food safety applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109756"},"PeriodicalIF":3.7,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0