Biochemical Engineering Journal最新文献

Removal of selenate from wastewater using a bioelectrochemical reactor: The importance of measuring selenide and the role of competing anions 利用生物电化学反应器去除废水中的硒酸盐：测量硒化物的重要性和竞争阴离子的作用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-15 DOI: 10.1016/j.bej.2024.109531

{"title":"Removal of selenate from wastewater using a bioelectrochemical reactor: The importance of measuring selenide and the role of competing anions","authors":"","doi":"10.1016/j.bej.2024.109531","DOIUrl":"10.1016/j.bej.2024.109531","url":null,"abstract":"<div><div>Removal of selenate (SeO<sub>4</sub><sup>2-</sup>) from selenate-contaminated wastewater is challenging due to the commonly co-existing and competing anions of sulfate (SO<sub>4</sub><sup>2-</sup>) and nitrate (NO<sub>3</sub><sup>-</sup>). This study investigates SeO<sub>4</sub><sup>2−</sup> reduction to elemental selenium (Se<sup>0</sup>) in a cathode-based bioelectrochemical (BEC) reactor and a conventional biofilm reactor (<em>i.e.</em>, an upflow anaerobic reactor). The simulated wastewater contained SeO<sub>4</sub><sup>2−</sup> at a typical concentration of 5 mg Se/L, SO<sub>4</sub><sup>2−</sup> at a typical concentration of 1000 mg S/L, and NO<sub>3</sub><sup>−</sup> at concentrations that varied from 0 to 10 mg N/L. The impact of sulfate on the BEC reactor was much lower than that on the conventional reactor: The selenium removal, defined as (selenate in influent – dissolved selenium in effluent)/selenate in influent, was 99 % in the BEC reactor versus 69 % in the conventional biofilm reactor. The lower selenium removal in the conventional reactor was mainly due to the >10 times higher reduction of sulfate, which directly caused competition between sulfate and selenate for the common resources such as electrons. The more reduction of sulfate in the conventional reactor further led to 45 times higher production of selenide. Selenide is usually assumed to be minimal and therefore not measured in the literature. This simplification may significantly overestimate selenium removal when the influent sulfate concentration is very high. NO<sub>3</sub><sup>-</sup> in the influent of the BEC reactor promoted selenium removal when it was less than 5.0 mg N/L but inhibited selenate removal when it was more than 7.5 mg N/L. This was supported by the microbial community analysis and intermediate (nitrite) analysis.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corn stover waste preparation cerium-modified biochar for phosphate removal from pig farm wastewater: Adsorption performance and mechanism 玉米秸秆废料制备的铈改性生物炭用于去除养猪场废水中的磷酸盐：吸附性能和机理

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-13 DOI: 10.1016/j.bej.2024.109530

{"title":"Corn stover waste preparation cerium-modified biochar for phosphate removal from pig farm wastewater: Adsorption performance and mechanism","authors":"","doi":"10.1016/j.bej.2024.109530","DOIUrl":"10.1016/j.bej.2024.109530","url":null,"abstract":"<div><div>The high phosphorus content in livestock and poultry wastewater is a significant factor contributing to water eutrophication. It is imperative to seek an economically efficient method for phosphate recovery. This study employed cerium-modified biochar to recover phosphate from pig farm wastewater. An investigation was conducted to examine the adsorption performance and removal mechanism of phosphate. Among the different samples, 0.1CeB-500℃ was selected for subsequent experiments. It exhibited a phosphate adsorption capacity of 9.58 mg/g and a removal efficiency of 95.75 %. The results showed that the phosphate adsorption process followed not only the pseudo-second-order kinetic model, but also the Langmuir isotherm model. It suggested that the adsorption of phosphate onto the biochar occurred in a monolayer chemical manner, with a maximum adsorption capacity of 10.86 mg/g. Phosphate adsorption was minimally affected within the pH range of 2–9, with Cl<sup>-</sup> having negligible impact, NO<sub>3</sub><sup>-</sup> slightly inhibiting, and HCO<sub>3</sub><sup>-</sup> and CO<sub>3</sub><sup>2-</sup> significantly hindering phosphate adsorption. A series of characterization results indicated that phosphate removal occurred through surface precipitation forming CePO<sub>4</sub>, ligand exchange between carbonate and phosphate, inner-sphere complexation, and electrostatic attraction. The phosphate removal efficiency from pig farm wastewater was 43.25 %, demonstrating promising potential for practical application.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface display of glycosyltransferase PgM8 and whole-cell catalysis for efficient Rebaudioside D biosynthesis in Pichia pastoris 糖基转移酶 PgM8 的表面显示和全细胞催化在 Pichia pastoris 中实现高效的 Rebaudioside D 生物合成

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-11 DOI: 10.1016/j.bej.2024.109522

引用次数: 0

Unraveling the residual sludge-mediated waste transformation and physiological regulation mechanism of Tetradesmus obliquus 揭示剩余污泥介导的废物转化和斜管四孢蘑菇的生理调节机制

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-11 DOI: 10.1016/j.bej.2024.109529

{"title":"Unraveling the residual sludge-mediated waste transformation and physiological regulation mechanism of Tetradesmus obliquus","authors":"","doi":"10.1016/j.bej.2024.109529","DOIUrl":"10.1016/j.bej.2024.109529","url":null,"abstract":"<div><div>The accumulation of residual sludge as process waste from water treatment engineering needs to be addressed urgently. <em>Tetradesmus obliquus</em> is an important algal species in the field of wastewater treatment. In this study, <em>T. obliquus</em> was cultured in different sludge extract to determine its ability to utilize wastes from the liquid phase and convert them into biomass, and to analyze the response of the microalgae to toxic stress using proteomics. The results showed that the sludge extract medium was superior to the BG11 medium in accumulating biomass, with dry weights, proteins and polysaccharides at least 1.09, 1.12 and 1.28 times higher than those of BG11 medium. In toxic group, <em>T. obliquus</em> reduced TOC from an initial 426.8±20.0 mg/L to 180.4±8.5 mg/L with a simultaneous 48.4 % reduction in toxicity. Toxic sludge extract produced greater damage to the photosystem of <em>T. obliquus</em> compared to the blank, significantly inhibiting the expression of two photosystem II core proteins, A0A383VSL5 (0.290 down) and A0A383V2Z3 (0.308 down), on day 5. However, these impairments were reversible, and at day 20, the expression of A0A383VSL5 was not inhibited, the inhibitory effect of A0A383V2Z3 (0.575 down) was attenuated. These results fill a gap on the treatment of various types of residual sludge by <em>T. obliquus</em> and provide promising strategies for microalgae treatment of residual sludge, whether non-toxic or toxic.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UV-modified biochar-Bacillus subtilis composite: An effective method for enhancing Cd(II) adsorption from water 紫外线改性生物炭-枯草芽孢杆菌复合材料：增强水中镉(II)吸附的有效方法

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-11 DOI: 10.1016/j.bej.2024.109527

{"title":"UV-modified biochar-Bacillus subtilis composite: An effective method for enhancing Cd(II) adsorption from water","authors":"","doi":"10.1016/j.bej.2024.109527","DOIUrl":"10.1016/j.bej.2024.109527","url":null,"abstract":"<div><div>The adsorption of Cd(Ⅱ) in sewage by single-modified biochar systems have limitations, whereas composite modification can enhance the efficiency. In this study, reed straw biochar and <em>Bacillus subtilis</em> were used as raw materials. UV radiation was employed to modify the biochar, and subsequently, <em>Bacillus subtilis</em> was loaded onto the biochar by adsorption, creating modified biochar composites. The Cd(II) adsorption performance and removal efficiency of these composites were then investigated. It was characterized by BET, SEM-EDS, FT-IR, XRD and ZETA potential analysis. Adsorption experiments were conducted under varying conditions (initial Cd(Ⅱ) concentration, UV radiation time, initial pH, etc.), with adsorption isotherms and kinetic models used. Results indicated that 24 hours UV radiation significantly enhanced adsorption performance, increasing the biochar’s surface area by 40 % and pore volume by 20 %, and introducing numerous pores and oxygen-containing functional groups to the biochar's surface. Significantly enhancing the saturation adsorption capacity for Cd(II) from 23.98 mg/g to 49.93 mg/g after UV- Modified biochar was loaded with <em>Bacillus</em>. Modified biochar composites performed better compared to single-modified biochar across different initial Cd(Ⅱ) concentrations, particularly in slightly alkaline environments. The primary adsorption mechanisms were chemical adsorption, such as ion exchange and surface precipitation. The synergistic effect of UV radiation and microbial loading significantly enhanced Cd(Ⅱ) adsorption efficiency. This study demonstrates that composite modification is a more efficient method, aiding in the removal of heavy metal ion Cd(Ⅱ) from water.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-cell synthesis of nicotinamide mononucleotide by recombinant Saccharomyces cerevisiae from glucose and nicotinamide 重组酿酒酵母从葡萄糖和烟酰胺全细胞合成烟酰胺单核苷酸

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-10 DOI: 10.1016/j.bej.2024.109528

{"title":"Whole-cell synthesis of nicotinamide mononucleotide by recombinant Saccharomyces cerevisiae from glucose and nicotinamide","authors":"","doi":"10.1016/j.bej.2024.109528","DOIUrl":"10.1016/j.bej.2024.109528","url":null,"abstract":"<div><div>β-Nicotinamide mononucleotide is recognized as a significant bioactive nucleotide, which is can be used in the fields of health industries. Many studies on the synthesis of NMN have involved <em>Escherichia coli</em> and the current methods are limited by safety problems as well as the expense of the substrate. Herein, GRAS-grade <em>Saccharomyces cerevisiae</em> was chosen as chassis cells to synthesize NMN using the substrates glucose and nicotinamide. First, the gene for the key enzyme nicotinamide phosphoribosyltransferase (<em>Nampt</em>) was screened from different sources, and site-directed mutation was performed to improve the synthesis of NMN. The concentration of intracellular NMN in yeast expressing the D83N-<em>Nampt</em> mutant derived from <em>Chitinophaga pinensis</em> reached 413.4 mg/L, which was 3.7 times higher than that of yeast expressing wild enzymes. The synthesis of NMN was further enhanced by overexpressing <em>Nampt</em> combined with weakening of the further metabolism of NMN. Subsequently, the supply of precursor phosphate ribose pyrophosphate (PRPP) was increased by overexpressing the PRPP synthase mutant, which led to the concentration of intracellular NMN increased to 775.9 mg/L from 537.8 mg/L. Finally, the concentration of intracellular NMN reached 1.2 g/L at 6 h after whole-cell catalytic optimization, which is the highest titer achieved by <em>S. cerevisiae</em> from inexpensive substrate glucose and nicotinamide. This study provides the synthesis of NMN by <em>S. cerevisiae</em> with a new and promising method.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into Pb (II) adsorption mechanisms using jackfruit peel biochar activated by a hydrothermal method toward heavy metal removal from wastewater 利用水热法活化的菠萝皮生物炭去除废水中重金属的铅 (II) 吸附机理探析

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-10 DOI: 10.1016/j.bej.2024.109525

{"title":"Insights into Pb (II) adsorption mechanisms using jackfruit peel biochar activated by a hydrothermal method toward heavy metal removal from wastewater","authors":"","doi":"10.1016/j.bej.2024.109525","DOIUrl":"10.1016/j.bej.2024.109525","url":null,"abstract":"<div><div>Understanding adsorption mechanisms plays an instrumental role in designing and operating adsorption-based wastewater treatment systems. This research systematically demonstrated the comprehensive adsorption mechanism of Pb(II) ions onto biochar synthesized from jackfruit peel in an aqueous solution using theoretical adsorption models and cutting-edge analytical techniques, such as FT-IR, TG-DSC, and SEM-EDX. The results showed that the adsorption process followed the Redlich-Peterson isothermal model and the intraparticle diffusion kinetic model under optimized conditions. The key mechanisms contributing to effective Pb(II) adsorption include complexation, ion - exchange, and intradiffusion. Furthermore, using the hydrothermal method to active biochar improves the surface area of JPT, leading to the maximum Pb(II) adsorption capacity of jackfruit peel-derived biochar to be 83.86 mg/g, higher than biochars from other parts of jackfruit waste (seeds and stems) and some materials from different agricultural residues in previous studies. These findings contribute to narrowing the gap in understanding heavy metal adsorption using biomass residues, theoretical models, and their mechanisms. Additionally, these indicate that agricultural by-products such as jackfruit peel are environmentally friendly and economical materials for implementing strategies aimed at mitigating heavy metal pollution in wastewater.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the dynamic viscosity of cell cultures and its effect on mixing performance in a spinner flask bioreactor 细胞培养物动态粘度的特征及其对旋转瓶生物反应器混合性能的影响

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-09 DOI: 10.1016/j.bej.2024.109523

{"title":"Characterization of the dynamic viscosity of cell cultures and its effect on mixing performance in a spinner flask bioreactor","authors":"","doi":"10.1016/j.bej.2024.109523","DOIUrl":"10.1016/j.bej.2024.109523","url":null,"abstract":"<div><div>Computational fluid dynamics (CFD) models have been developed to simulate cell culturing bioreactors but assume water-like viscosity properties due to significant data gaps. This study characterized the dynamic viscosity of HEK-293 cell cultures and evaluated its effect on mixing performance in a spinner flask bioreactor. Viscosity measurements indicated that the cell culture media, media with microcarriers, and cell cultures presented shear thinning behaviors within the measured shear rate range of 1 to 100s<sup>-1</sup>. The viscosity also increased with the microcarrier concentrations and growth of cell culture. The CFD model, incorporating dynamic viscosity data, showed that shear stress and Kolmogorov length profiles are significantly influenced by microcarrier concentrations and cell culture growth. Higher microcarrier concentrations led to higher average shear stress and Kolmogorov values. The cultured HEK-293 cells after seven days of growth also had higher average shear stress and Kolmogorov values than at the day of seeding, indicating an impact caused by the cells’ metabolism and biomass. Overall, the results indicated that assuming water-like properties underestimates shear stress and Kolmogorov length scales, especially at zones of lower shear rates due to the observed shear thinning behavior. Thus, careful monitoring of dynamic viscosity of cell cultures and proper control of mixing parameters are critical to deliver the desired mixing conditions for optimized cell growth especially during scale-up production operations.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating azo dye degradation and lipid accumulation by Candida tropicalis and Pichia kudriavzevii along with insights into underlying metabolomics for treatment of textile effluents 整合热带念珠菌和 Pichia kudriavzevii 降解偶氮染料和积累脂质的方法，以及对潜在代谢组学的深入了解，以处理纺织污水

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-09 DOI: 10.1016/j.bej.2024.109521

{"title":"Integrating azo dye degradation and lipid accumulation by Candida tropicalis and Pichia kudriavzevii along with insights into underlying metabolomics for treatment of textile effluents","authors":"","doi":"10.1016/j.bej.2024.109521","DOIUrl":"10.1016/j.bej.2024.109521","url":null,"abstract":"<div><div>Azo compounds, extensively utilized across various industries, contribute to the release of toxic effluents that are detrimental to both the environment and human health. Traditional methods for azo dye removal often result in harmful byproducts or concentrated sludge, complicating disposal efforts. This study explores the potential of two yeast strains, <em>Candida tropicalis</em> and <em>Pichia kudriavzevii</em>, to effectively decolorize azo dyes (TD4, TD5, and TD6) while simultaneously accumulating lipids. The cultures achieved 80–90 % decolorization of the selected dyes during incubation, with <em>Pichia</em> showing higher efficiency across multiple dyes compared to <em>Candida</em>. Lipid profiling identified valuable fatty acids, such as palmitic acid and oleic acid, with potential applications in biofuels and other industries. Total Organic Carbon (TOC) analysis revealed a reduction in TOC, indicating degradation and mineralization of the dyes by the yeasts. Metabolic profiling via LC-MS confirmed the degradation, showing the presence of intermediates such as azoles, azolines, isoquinolines, pyridines, and benzopyrans in dye-supplemented cultures. Additionally, pathways related to energy metabolism, amino acid metabolism, drug metabolism (cytochrome P450), degradation of aromatic compounds, and steroid biosynthesis were enriched in the dye-treated cultures. Lipid output in the presence of dyes ranged from 40 % to 90 %. The study thus demonstrates a proof of concept for economically viable lipid production combined with efficient dye removal, presenting a sustainable solution to environmental and industrial challenges.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined optical measurement of dissolved oxygen tension (DOT), pH value, biomass and viscosity in shake flasks 在摇瓶中对溶解氧张力 (DOT)、pH 值、生物量和粘度进行组合光学测量

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-05 DOI: 10.1016/j.bej.2024.109515

{"title":"Combined optical measurement of dissolved oxygen tension (DOT), pH value, biomass and viscosity in shake flasks","authors":"","doi":"10.1016/j.bej.2024.109515","DOIUrl":"10.1016/j.bej.2024.109515","url":null,"abstract":"<div><div>Shake flasks are widely spread in microbial process development. Characterization of the processes by manual offline sampling is time-consuming, highly laborious and a contamination risk. Online monitoring of key parameters would provide deeper insights, while saving time and effort. In this study, a device for optical online monitoring of dissolved oxygen tension (DOT), biomass, pH value and viscosity in shake flasks is presented. DOT measurement relies on fluorescent oxygen sensitive nanoparticles. The fluorescence intensity signal of the nanoparticles is used to trigger the DOT and scattered light measurements inside the rotating bulk liquid. The scattered light signal (610 – 630 nm) can be correlated to offline measured optical density OD<sub>600</sub>, even at elevated viscosity. The pH value is monitored online by using pH sensor spots, fixed inside the shake flasks. The shift of the angle of the bulk liquid Θ-Θ<sub>0</sub> is correlated to the offline measured viscosity. Detection of the leading edge of the bulk liquid, necessary for viscosity measurement, can be performed either using the fluorescence intensity signal of the oxygen nanoparticles or the scattered light signal.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0