Biochemical Engineering Journal最新文献

Liquid paraffin gas carrier enhanced the HOB microbial protein production in a bubble column reactor with a gas-liquid mixing pump

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-31 DOI: 10.1016/j.bej.2025.109737

Wenfang Cai , Xiaoying Shen , Jiayao Gao , Bingyan Wang , Xueqi Wang , Yaze Li , Jinzhe Liu , Yunhai Wang , Kun Guo

{"title":"Liquid paraffin gas carrier enhanced the HOB microbial protein production in a bubble column reactor with a gas-liquid mixing pump","authors":"Wenfang Cai , Xiaoying Shen , Jiayao Gao , Bingyan Wang , Xueqi Wang , Yaze Li , Jinzhe Liu , Yunhai Wang , Kun Guo","doi":"10.1016/j.bej.2025.109737","DOIUrl":"10.1016/j.bej.2025.109737","url":null,"abstract":"<div><div>Microbial protein (MP) production through autotrophically hydrogen-oxidizing bacterium (HOB) has been regarded as a potential technology for feed or food supply. However, due to the low solubility of O<sub>2</sub> and H<sub>2</sub>, how to match the biotic O<sub>2</sub> and H<sub>2</sub> consumption rate and the transfer kinetics presents the key challenge to achieve desired HOB protein production. Here, we introduce liquid paraffin as a gas carrier in a bubble column reactor with a self-priming gas-liquid mixing pump for HOB MP production. The self-priming gas-liquid mixing pump facilitates the efficiency of gas-liquid mixing, while the liquid paraffin improves the solubility of O<sub>2</sub> and H<sub>2</sub>. This combined effect optimizes the kinetics of O₂ and H₂ transfer, resulting in an increase in the K<sub>L</sub>a from 0.69 to 1.81 h<sup>−1</sup> for O<sub>2</sub>, and from 0.37 to 1.67 h<sup>−1</sup> for H<sub>2</sub>. Consequently, the yield of NH<sub>4</sub><sup>+</sup>-N to HOB protein increased from 4.21 ± 0.18 gMP∙gN<sup>−1</sup> to 5.18 ± 0.68 gMP∙gN<sup>−1</sup>, and an average protein content of 53 ± 5 % in dried biomass was achieved with nearly 100 % O<sub>2</sub> and H<sub>2</sub> uptake after adding liquid paraffin. The essential amino profile analysis shows that the presence of liquid paraffin has no remarkably impact on the protein quality. These results demonstrate that integration of gas carrier and reactor design is viable to address the gas transfer limitation in the MP production.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"219 ","pages":"Article 109737"},"PeriodicalIF":3.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimal dosage and recycle of cellulases for efficient biomass saccharification of cardoon and giant reed

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-29 DOI: 10.1016/j.bej.2025.109735

Adriana Posilipo , Maria Elena Russo , Piero Salatino , Antonio Marzocchella

{"title":"Optimal dosage and recycle of cellulases for efficient biomass saccharification of cardoon and giant reed","authors":"Adriana Posilipo , Maria Elena Russo , Piero Salatino , Antonio Marzocchella","doi":"10.1016/j.bej.2025.109735","DOIUrl":"10.1016/j.bej.2025.109735","url":null,"abstract":"<div><div>The deployment of sugar-based biorefineries is limited by the availability of feedstocks and by the cost of enzymes. <em>Cynara cardunculus</em> and <em>Arundo donax</em> are relevant non-food crops for the development of sugar-based biorefineries. Enzymatic hydrolysis of <em>C. cardunculus</em> and <em>A. donax</em> stalks have been optimized in terms of biocatalyst dosage and reuse. Optimal utilization of a commercial biocatalyst cocktail was achieved assessing the enzyme adsorption on the biomass slurry prior to the hydrolysis stage. The partitioning of cellulases between the liquid the substrate has been characterized for raw and pretreated biomasses. Enzyme uptakes up to 18 mg/g were recorded with 5 % biomass slurries and 0.2–3 g/L initial enzyme concentrations for both <em>C. cardunculus</em> and <em>A. donax</em>. The nearly irreversible nature of the enzyme adsorption allowed a partial recovery of the unbound biocatalyst and the polysaccharides hydrolysis by adsorbed biocatalysts. In the best condition, 89 % glucans conversion was obtained in pretreated cardoon with 4 mg/g loading of adsorbed cellulases (lower than the saturation level) and in giant reed with 13 mg/g. Notably, the residual activity of the unbound biocatalyst was sufficient to hydrolyse more than 65 and 75 % of glucans in pretreated cardoon and giant reed, respectively. The proposed method can be applied routinely to any biorefinery feedstock and both commercial and <em>in-situ</em> produced cocktails to minimize the enzyme dosage.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"219 ","pages":"Article 109735"},"PeriodicalIF":3.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-pot synthesis of arginine decarboxylase@ZIF-8 using intrinsic pyridoxal 5′-phosphate cofactor for agmatine production

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-28 DOI: 10.1016/j.bej.2025.109730

Yan Wang, Bo Ding, Qingqing Bao, Yunying Zhao, Guohui Li, Yu Deng

{"title":"One-pot synthesis of arginine decarboxylase@ZIF-8 using intrinsic pyridoxal 5′-phosphate cofactor for agmatine production","authors":"Yan Wang, Bo Ding, Qingqing Bao, Yunying Zhao, Guohui Li, Yu Deng","doi":"10.1016/j.bej.2025.109730","DOIUrl":"10.1016/j.bej.2025.109730","url":null,"abstract":"<div><div>Agmatine is an important basic drug and nutritional supplements, which exhibits significant physiological activities. Traditional methods, such as whole-cell catalysis, require substantial pyridoxal 5′-phosphate (PLP) addition, which accounts for 15 % of production costs. In this study, we utilized purified arginine decarboxylase (AdiA-M2) that leverages the intrinsic PLP cofactor, without the need for additional PLP supplementation, and ZIF-8 was employed to enhance the pH tolerance. The successful incorporation of AdiA-M2 into the ZIF-8 structure was confirmed through scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The immobilized arginine decarboxylase demonstrated markedly enhanced performance under alkaline conditions, along with improved thermal stability and storage activity compared to its free enzyme counterpart. Specifically, the immobilized AdiA@ZIF-8 system achieved an initial conversion rate of 95.16 %, after five recycles the titer attained 266.69 g/L without the extra PLP addition, which is 1.67 times higher than the best current production approach. The adopted enzyme-catalysis system eliminated the need for additional cofactors, thereby lowering costs and providing valuable insights for the industrial production of agmatine.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"219 ","pages":"Article 109730"},"PeriodicalIF":3.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient degradation of sulfamonomethoxine in wastewater using a novel intimately coupled photocatalysis and biodegradation system prepared with the calcium alginate hydrogel

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-27 DOI: 10.1016/j.bej.2025.109731

Lai Peng , Qianyu Long , Chuanzhou Liang , Linchuan Fang , Yifeng Xu

{"title":"Efficient degradation of sulfamonomethoxine in wastewater using a novel intimately coupled photocatalysis and biodegradation system prepared with the calcium alginate hydrogel","authors":"Lai Peng , Qianyu Long , Chuanzhou Liang , Linchuan Fang , Yifeng Xu","doi":"10.1016/j.bej.2025.109731","DOIUrl":"10.1016/j.bej.2025.109731","url":null,"abstract":"<div><div>The insecure fixation of photocatalysts and their susceptibility to being covered by microorganisms existed in the traditional intimately coupled photocatalysis and biodegradation (ICPB) system. In this work, an ICPB system was developed by coupling g-C<sub>3</sub>N<sub>4</sub> and biofilm carriers through introducing calcium alginate (CA) hydrogel. The ICPB-CA system showed superior removal efficiencies of sulfamonomethoxine (SMM) (99.3 %) compared to corresponding photocatalysis and biodegradation systems. Relatively higher stability was also achieved with SMM removal efficiencies higher than 92.5 % after three consecutive cycles, as CA hydrogel markedly improved the adhesion strength between the photocatalysts and biofilm carriers. Furthermore, SMM degradation pathways in the ICPB-CA system were proposed, with smaller-molecular products produced. Biotoxicity evaluation tests also indicated that the degradation products were less toxic than the parent SMM, suggesting that ICPB-CA system could significantly mitigate the antimicrobial activities of SMM. This study will provide a promising alternative to construct the ICPB system, realizing higher removal efficiency of antibiotics while decreasing their toxicity.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"219 ","pages":"Article 109731"},"PeriodicalIF":3.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research progress and application prospects of microbial mineralization for repairing cracks in recycled concrete

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-27 DOI: 10.1016/j.bej.2025.109733

Yingjie Chen , Yuping Chen , Shuyong Wang , Zhenbin Chen , Jianbo Guo , Guoxin Chen

{"title":"Research progress and application prospects of microbial mineralization for repairing cracks in recycled concrete","authors":"Yingjie Chen , Yuping Chen , Shuyong Wang , Zhenbin Chen , Jianbo Guo , Guoxin Chen","doi":"10.1016/j.bej.2025.109733","DOIUrl":"10.1016/j.bej.2025.109733","url":null,"abstract":"<div><div>The accumulation of construction waste imposes a significant burden on the environment. Recycling construction waste into concrete using recycled aggregate has emerged as an effective solution. However, the poor mechanical properties of recycled concrete limit its widespread application. Microbial mineralization-induced calcium carbonate precipitation (MICP) has been proposed as an environmentally friendly repair method to enhance the performance of recycled concrete. Although this technology demonstrates considerable potential, practical applications face several challenges. This paper systematically reviews the key issues in this field and evaluates existing solutions, highlighting their advantages and limitations. The primary challenge is bacterial survival, which has been addressed through two main strategies: (1) enhancing bacterial adaptability via domestication and (2) modifying the external environment by reducing pH and applying protective shells. This dual-protection approach improves bacterial viability and mitigates external environmental interference. The integration of microbial mineralization with recycled concrete is not only environmentally sustainable but also economically advantageous, reducing initial capital investment by 50 % compared to conventional remediation methods while significantly lowering maintenance costs. Additionally, incorporating artificial intelligence into microbial concrete enables real-time monitoring and process optimization, offering an innovative pathway for sustainable construction.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"219 ","pages":"Article 109733"},"PeriodicalIF":3.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Responses of nitrogen removal, microbial community and antibiotic resistance genes to biodegradable microplastics during biological wastewater treatment

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-25 DOI: 10.1016/j.bej.2025.109732

Yiwei Zhou , Shaochen Bian , Hua Wang , Yixuan Chu , Lei Zheng , Yali Song , Chengran Fang

{"title":"Responses of nitrogen removal, microbial community and antibiotic resistance genes to biodegradable microplastics during biological wastewater treatment","authors":"Yiwei Zhou , Shaochen Bian , Hua Wang , Yixuan Chu , Lei Zheng , Yali Song , Chengran Fang","doi":"10.1016/j.bej.2025.109732","DOIUrl":"10.1016/j.bej.2025.109732","url":null,"abstract":"<div><div>Biodegradable plastics decompose more easily into microplastics (MPs) as alternatives to traditional plastics. However, the effects of biodegradable microplastics (BMPs) on the aerobic biological treatment process in activated sludge need further investigation. This study compared the effects of three BMPs (Polylactic acid (PLA), Polyhydroxyalkanoate (PHA) and Polybutylene succinate (PBS)) with 1, 10 and 50 mg/L on nitrogen removal, microbial community and antibiotic resistance genes (ARGs) in activated sludge. The results demonstrated that 10 and 50 mg/L PHA and 50 mg/L PLA enhanced specific nitrate and specific nitrite reduction rates, promoting total nitrogen removal. The positive effects on denitrification-related functional genes (such as <em>napA, nirK, nirS</em>) were also identified. Additionally, 10 mg/L PLA and PHA stimulated the production of loosely bound extracellular polymeric substances (EPS). However, 50 mg/L PLA and PHA decreased the tightly and loosely bound EPS contents. The response of nitrogen removal and EPS were attributed to the shifts of dominant bacteria abundance including <em>Bacteroidota, Chloroflexi</em> and <em>Acidobacteriota.</em> Moreover, PHA and PLA promoted the abundance of ARGs and <em>intI1</em> in activated sludge, particularly at high concentrations. However, PBS did not affect the nitrogen removal, EPS and microbial communities. The results might shed light on the influence of BMPs on activated sludge.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"219 ","pages":"Article 109732"},"PeriodicalIF":3.7,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing anaerobic digestion performance of food-waste solid residue through alkali-thermal pretreatment

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-23 DOI: 10.1016/j.bej.2025.109726

Tianru Wang , Jing He , Junwei He , Tisen Xiao , Qing Liu

{"title":"Enhancing anaerobic digestion performance of food-waste solid residue through alkali-thermal pretreatment","authors":"Tianru Wang , Jing He , Junwei He , Tisen Xiao , Qing Liu","doi":"10.1016/j.bej.2025.109726","DOIUrl":"10.1016/j.bej.2025.109726","url":null,"abstract":"<div><div>The resource utilization of food-waste solid residue has received much attention, but the high organic content has resulted in poor hydrolysis performance during anaerobic digestion. In this study, pretreated food-waste solid residues through alkali, thermal and alkali-thermal were mixed with excess sludge for anaerobic digestion. Results indicated that pretreatments significantly alleviated the inhibitory effect of NH<sub>4</sub><sup>+</sup>-N and stabilized the pH of the digestive environment. Under the condition of alkali-thermal group, the concentrations of soluble chemical oxygen demand (SCOD), soluble protein, soluble polysaccharide and Volatile fatty acids (VFAs) were degraded by 81.00 %, 53.02 %, 42.87 % and 99.99 %, respectively, compared to the condition of control group. The absolute abundance advantage of <em>Dojkabacteria</em> significantly reduced the risk of ammonia inhibition and the relative abundance of <em>Methanobacterium</em> archaea was nearly 90 % under the condition of alkali-thermal group. It was observed that substrate was fragmented and enriched with bacilli and cocci in the crevices after the reaction under the condition of alkali-thermal group. The pretreatment was effective in utilizing more DOM, thereby increasing the efficiency of methane production. The condition of alkali-thermal group had the highest methanogenic potential, which was 16.78 % and 34.04 % more than alkali group and thermal group, respectively, and the maximum methane production rate was at its highest (<em>R</em><sub><em>max</em></sub> =522.70 mL/d). Compared to the condition of control group, the cumulative methane productions increased by 685.90 %, 593.71 % and 847.07 % under the conditions of alkali group, thermal group and alkali-thermal group, respectively. This founding provided an effective way for resource utilization of food-waste solid residue.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"219 ","pages":"Article 109726"},"PeriodicalIF":3.7,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improvement of alka(e)ne production in Escherichia coli by the 3′-UTR engineering of acyl-ACP reductase

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-20 DOI: 10.1016/j.bej.2025.109725

Jiahu Han, Takuya Matsumoto, Ryosuke Yamada, Hiroyasu Ogino

{"title":"Improvement of alka(e)ne production in Escherichia coli by the 3′-UTR engineering of acyl-ACP reductase","authors":"Jiahu Han, Takuya Matsumoto, Ryosuke Yamada, Hiroyasu Ogino","doi":"10.1016/j.bej.2025.109725","DOIUrl":"10.1016/j.bej.2025.109725","url":null,"abstract":"<div><div>The sustainable and efficient production of biofuels has generated considerable interest in the microbial synthesis of alka(e)nes, which are promising alternatives to fossil fuels. Acyl-ACP reductase (AAR) is a critical enzyme in the alka(e)ne biosynthetic pathway, and its poor solubility in <em>Escherichia coli</em> is a major bottleneck during the optimization of production yields. The approaches for enhancing protein solubility typically include the fusion of solubility tags at the N- or C-termini of target proteins, which can sometimes interfere with protein function or stability. The present study developed a novel strategy that leverages the regulatory potential of 3′-untranslated regions (3′-UTRs) by integrating the sequence coding thioredoxin (Trx), small ubiquitin-like modifier (SUMO), maltose-binding protein (MBP), or N-utilization substance protein A (NusA), into the 3′-UTR of the <em>AAR</em> gene. The strategy aimed to enhance the stability of AAR mRNA for improving the solubility and expression of AAR without altering its primary structure. The findings revealed that this strategy significantly enhanced the solubility and expression levels of AAR in <em>Escherichia coli</em>, which markedly increased alka(e)ne production. This method has potential widespread applications in metabolic engineering and synthetic biology. The study paves the way for the development of more efficient strategies aimed at producing biofuels, and highlights the untapped potential of the 3′-UTR engineering strategy.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"219 ","pages":"Article 109725"},"PeriodicalIF":3.7,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automated regression of bioreactor models using a Bayesian approach for parallel cultivations in robotic platforms

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-19 DOI: 10.1016/j.bej.2025.109729

Martin F. Luna , Federico M. Mione , Lucas Kaspersetz , Peter Neubauer , Ernesto C. Martinez , M. Nicolas Cruz Bournazou

{"title":"Automated regression of bioreactor models using a Bayesian approach for parallel cultivations in robotic platforms","authors":"Martin F. Luna , Federico M. Mione , Lucas Kaspersetz , Peter Neubauer , Ernesto C. Martinez , M. Nicolas Cruz Bournazou","doi":"10.1016/j.bej.2025.109729","DOIUrl":"10.1016/j.bej.2025.109729","url":null,"abstract":"<div><div>Mathematical models of bioreactors are powerful tools that aid in the analysis and prediction of process operation. However, the complex behavior of microorganisms makes modelling of biological processes a particularly challenging task, especially in the early developmental stages when data and knowledge are scarce. As a result, bioreactor models may perform poorly due to structural errors or high uncertainty in their parameterization. Here, we present a method for automated dynamic model regression based on a Bayesian approach that can be applied in the operation of laboratory robotic platforms to perform both parameter estimation and state predictions for a given experimental design. Starting with wide distributions over parameters (prior knowledge), the model is updated as new data is generated and is then used to predict the evolution of the experiment. The proposed method is tested with data from several parallel cultivations from a 24 mini-bioreactors platform containing an <em>Escherichia coli</em> strain operating in fed-batch mode. The results highlight both the versatility of the approach to estimate parameter distribution as well as to predict the state evolution.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"219 ","pages":"Article 109729"},"PeriodicalIF":3.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of stringent stress response and amino acid supplementation on recombinant protein production in Escherichia coli

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-17 DOI: 10.1016/j.bej.2025.109727

Girish H. Rajacharya , Jashwant Kumar , Jaya A. Gupta , Anurag S. Rathore

{"title":"Impact of stringent stress response and amino acid supplementation on recombinant protein production in Escherichia coli","authors":"Girish H. Rajacharya , Jashwant Kumar , Jaya A. Gupta , Anurag S. Rathore","doi":"10.1016/j.bej.2025.109727","DOIUrl":"10.1016/j.bej.2025.109727","url":null,"abstract":"<div><div>The metabolic response of <em>Escherichia coli</em> BL21 DE3 strains upon amino acid supplementation and stringent stress was examined to assess its effects on growth, protein production, metabolic efficiency, and cellular stress. In the absence of amino acids, <em>E. coli</em> prioritized amino acid synthesis, essential for recombinant protein production. Supplementation led to faster growth, higher cell densities, and reduced acetate accumulation, indicating improved metabolic balance. Proteomic analysis revealed notable changes in protein abundance during the early log and post-induction mid-log phases, with supplemented cultures showing enhanced metabolic capacity and reduced stress levels. Gene expression analysis demonstrated that amino acid availability downregulated stress response genes and upregulated genes associated with nutrient uptake and metabolism, boosting growth and productivity. Specifically, genes <em>lldR</em>, <em>ydgR</em>, <em>tppB</em>, and <em>yhhN</em> were upregulated, while <em>adiA</em> and <em>ydeI</em> were downregulated, reflecting better metabolic conditions. Stringent response analysis showed elevated (p)ppGpp levels and increased activities of SpoT and RelA without supplementation, indicative of nutrient stress. Supplementation lowered these stress markers, fostering a favourable metabolic state. The study underscores the importance of amino acid supplementation for optimizing biomass production and recombinant protein yields, offering insights into <em>E. coli</em> physiology and strategies for metabolic engineering.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"219 ","pages":"Article 109727"},"PeriodicalIF":3.7,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143685697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0