Biochemical Engineering Journal最新文献

Ammonia oxidation coupled nitrate reduction driven by bio-iron sludge in low-carbon mode: Performance and mechanisms 低碳模式下生物铁污泥驱动氨氧化耦合硝酸盐还原：性能与机制

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-10-03 DOI: 10.1016/j.bej.2025.109951

Lihong Zhang , Hongrong Zhao

{"title":"Ammonia oxidation coupled nitrate reduction driven by bio-iron sludge in low-carbon mode: Performance and mechanisms","authors":"Lihong Zhang , Hongrong Zhao","doi":"10.1016/j.bej.2025.109951","DOIUrl":"10.1016/j.bej.2025.109951","url":null,"abstract":"<div><div>Fe(III)-reduction coupled NH<sub>4</sub><sup>+</sup>-N oxidation (Feammox) and Fe(II)-oxidation coupled NO<sub>x</sub><sup>-</sup>-N reduction (NDFO) were newly discovered, cost-effective biological nitrogen removal technologies in recent years. To determine the nitrogen removal efficiency under low-carbon mode, the AOCNR system was established, and its nitrogen removal performance and mechanisms were investigated under both zero-carbon (C/N = 0) and low-carbon (C/N = 0.5) conditions. The results demonstrated that TN removal efficiency significantly improved under low-carbon conditions, with a maximum removal capacity of 38.24 mg/L, including 18.65 mg/L of NH<sub>4</sub><sup>+</sup>-N oxidation and 87.9 % NO<sub>3</sub><sup>-</sup>-N reduction rate. Batch experiments confirmed that moderate increase in NO<sub>3</sub><sup>-</sup>-N could enhance NH<sub>4</sub><sup>+</sup>-N oxidation, while excessive concentrations inhibited NO<sub>3</sub><sup>-</sup>-N reduction. External iron supplementation (30 g/L) markedly improved NO₃⁻-N removal but might lead to NH<sub>4</sub><sup>+</sup>-N accumulation. Isotope tracing revealed the synergistic effect between Feammox and NDFO, and the main product of NH₄⁺-N oxidation was NO₂⁻-N. In addition, dominant functional genera (e.g., Thauera, Ferruginibacter) related to Fe(III)/Fe(II) cycle-driven nitrogen removal were detected in the AOCNR system.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"226 ","pages":"Article 109951"},"PeriodicalIF":3.7,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145218880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Numerical simulation of catalytic enhancement in wall-coated enzyme-catalyzed micro-channel via geometric design 基于几何设计的壁涂酶催化微通道催化强化数值模拟

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-10-01 DOI: 10.1016/j.bej.2025.109947

Yiruo He , Jun Liang , Huiting Xu , Zhixi Zhang , Weiyi Su , Yuqi Hu , Na Wang , Xiong Yu , Honghai Wang

{"title":"Numerical simulation of catalytic enhancement in wall-coated enzyme-catalyzed micro-channel via geometric design","authors":"Yiruo He , Jun Liang , Huiting Xu , Zhixi Zhang , Weiyi Su , Yuqi Hu , Na Wang , Xiong Yu , Honghai Wang","doi":"10.1016/j.bej.2025.109947","DOIUrl":"10.1016/j.bej.2025.109947","url":null,"abstract":"<div><div>Microfluidics is widely used in enzyme biotechnology. Wall-coated immobilized enzyme micro-channels (W-IEMRs) offer significant advantages in stability and hydrodynamic performance but have limited catalytic efficiency due to long diffusion paths in the microreactor. This study used a computational fluid dynamics (CFD) model to simulate flow fields and concentration distributions in serpentine micro-channels, quantifying mixing efficiency via the mixing index (MI) and Dean number (De). By testing channels with different curvature radius, bending ratios, and inner diameters, the geometry was optimized to enhance mass transfer and mixing. Simulations showed that a smaller curvature radius, larger bending ratio, and smaller inner diameter strengthen Dean vortices, improving mixing. However, catalytic efficiency has a non-monotonic relationship with these parameters: a curvature radius below 6.31 mm reduces enzyme-substrate contact due to excessive vortices, while a bending ratio exceeding 60 % leads to uneven substrate distribution caused by counter-rotating vortices in adjacent sections, impairing performance. Optimal design parameters are: curvature radius 6.31 mm, bending ratio 60 %, inner diameter 0.5 mm. For micro-channels with varying reaction kinetics, careful consideration must be given to balancing mass transfer and catalytic performance.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"226 ","pages":"Article 109947"},"PeriodicalIF":3.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145218879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adsorption characteristics of sludge-based composite biochar for norfloxacin and oxytetracycline in a binary system 污泥基复合生物炭在二元体系中对诺氟沙星和土霉素的吸附特性

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-09-29 DOI: 10.1016/j.bej.2025.109949

Xinhua Zhou, Ziqiang Ding, Zhihao Li, Xilin Li, Fankang Meng, Lin Lu, Letong Wang

{"title":"Adsorption characteristics of sludge-based composite biochar for norfloxacin and oxytetracycline in a binary system","authors":"Xinhua Zhou, Ziqiang Ding, Zhihao Li, Xilin Li, Fankang Meng, Lin Lu, Letong Wang","doi":"10.1016/j.bej.2025.109949","DOIUrl":"10.1016/j.bej.2025.109949","url":null,"abstract":"<div><div>Antibiotics are widely used and detected in water, posing environmental and health risks. This study prepared a sludge-based biochar composite (SBC-MMT) by co-pyrolysis of activated sludge and montmorillonite, and characterized it using SEM, FTIR, and XRD. Its adsorption performance for norfloxacin (NOR) and oxytetracycline (OTC) was evaluated in single and binary systems. In single systems at 1.20 g/L and pH 7, removal efficiencies reached 98.68 % for NOR and 85.86 % for OTC, with adsorption capacities of 106.43 mg/g and 88.54 mg/g, respectively. In binary systems at 1.20 g/L and pH 7, removal efficiencies decreased to 82.85 % for NOR and 68.65 % for OTC, with adsorption capacities of 67.04 mg/g and 54.42 mg/g, respectively, showing competitive adsorption with preference for NOR (K<sub>NOR</sub> > K<sub>OTC</sub>). Adsorption followed pseudo-second-order kinetics and the Freundlich model, indicating chemisorption on heterogeneous surfaces. Mechanisms included electrostatic attraction, hydrogen bonding, π-π interactions, and ion exchange. SBC-MMT embodies the “Treating Waste with Waste” concept. It is an effective, reusable adsorbent made from sludge. It provides valuable insights for optimizing wastewater treatment and reducing environmental risks.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"225 ","pages":"Article 109949"},"PeriodicalIF":3.7,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On a new approach to describing soft pellets of filamentous cultivated in shake flasks under turbulent flow conditions 一种描述湍流条件下摇瓶培养的丝状微球的新方法

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-09-29 DOI: 10.1016/j.bej.2025.109942

Mohammad Reza Kardooni , Jintian Liu , Zuzanna Kozanecka , Rainer Krull , Markus Böl

{"title":"On a new approach to describing soft pellets of filamentous cultivated in shake flasks under turbulent flow conditions","authors":"Mohammad Reza Kardooni , Jintian Liu , Zuzanna Kozanecka , Rainer Krull , Markus Böl","doi":"10.1016/j.bej.2025.109942","DOIUrl":"10.1016/j.bej.2025.109942","url":null,"abstract":"<div><div>When cultivating filamentous pellets, the mechanical behaviour during cultivation is of particular interest for high process productivity under turbulent hydrodynamic flow conditions. However, the exact mechanical interactions between the pellets or between the pellets and the reactor walls are still not fully understood and can hardly be resolved using experimental methods. This study therefore presents a combined numerical approach to investigating the mechanical behaviour of soft biological pellets in highly turbulent flows. Unlike conventional models, which focus on rigid particles, this approach captures the complex interactions between culture fluid and pellet, pellet and pellet, and pellet and reactor wall. The developed model efficiently calculates the conditions in shake flask cultivations while providing a realistic representation of them by modelling the motion of particles on a large scale and capturing the free surface. By incorporating turbulence-induced mechanical effects, the model sheds light on the impact of fluid and contact forces on changes in pellet morphology. These findings contribute to the optimisation of large-scale biotechnological processes, increasing productivity and operating efficiency in microbial cultivation systems.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"225 ","pages":"Article 109942"},"PeriodicalIF":3.7,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic analysis of the effects of nutritional conditions on Rhodosporidium toruloides lipid production 营养条件对环形红孢子虫脂质产生影响的转录组学分析

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-09-26 DOI: 10.1016/j.bej.2025.109945

Yulong Yang , Zi Ye , Minrui Guo , Guogang Chen

{"title":"Transcriptomic analysis of the effects of nutritional conditions on Rhodosporidium toruloides lipid production","authors":"Yulong Yang , Zi Ye , Minrui Guo , Guogang Chen","doi":"10.1016/j.bej.2025.109945","DOIUrl":"10.1016/j.bej.2025.109945","url":null,"abstract":"<div><div><em>Rhodosporidium toruloides</em> is an excellent oleaginous yeast that produces lipids that can be used for energy production and chemical industry, with enormous application potential and economic value. The lipid production capacity of <em>R. toruloides</em> is affected by many factors, among which carbon (C) source, nitrogen (N) source, and C/N ratio are the key influencing factors. Therefore, in this study, the growth and lipid synthesis ability of <em>R. toruloides</em> were evaluated under different nutritional conditions, and transcriptome sequencing was performed. The results showed that the T2 experimental group (sucrose, C/N = 200) exhibited the highest lipid production capacity, with a lipid yield of 6.43 ± 0.26 g/L and a lipid content of 67.32 %, compared with the CK control group (glucose, C/N = 20) and the T1 experimental group (sucrose, C/N = 20). RNA-Seq analysis was conducted on all three groups to investigate the underlying molecular mechanisms. Enrichment analysis showed that N limitation up-regulated the expression of genes related to triglyceride synthesis and fatty acid synthesis, down-regulated the expression of genes related to glycolysis, phosphopentose pathway and tricarboxylic acid cycle, and inhibited cell proliferation, leading to enhanced carbon flow to lipid synthesis. This study explored the metabolic relationship between nitrogen limitation and lipid synthesis in <em>R. toruloides</em>, providing insights for improving lipid production and its industrial application.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"225 ","pages":"Article 109945"},"PeriodicalIF":3.7,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient benzaldehyde degradation by Barnettozyma hawaiiensis and its application in shale gas fracturing flowback fluid treatment: Identification, performance, and mechanisms 夏威夷巴尼托菌高效降解苯甲醛及其在页岩气压裂返排液中的应用：鉴定、性能及机理

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-09-26 DOI: 10.1016/j.bej.2025.109944

Hongmei Yang , Yingxue Geng , Wenshi Gou , Ping Yang

{"title":"Efficient benzaldehyde degradation by Barnettozyma hawaiiensis and its application in shale gas fracturing flowback fluid treatment: Identification, performance, and mechanisms","authors":"Hongmei Yang , Yingxue Geng , Wenshi Gou , Ping Yang","doi":"10.1016/j.bej.2025.109944","DOIUrl":"10.1016/j.bej.2025.109944","url":null,"abstract":"<div><div>This study isolated a novel halotolerant and acid-tolerant yeast, Barnettozyma hawaiiensis, from raw shale gas fracturing flowback fluid (SGFFF), demonstrating high-efficiency degradation of benzaldehyde—a representative toluene-derived aromatic intermediate. Under optimized conditions (pH 5, 20 g/L NaCl, 35°C), it achieved 98.28 % benzaldehyde and 74.76 % total organic carbon(TOC) removal within 24 h. Whole-genome sequencing revealed abundant genes related to organic degradation, salt/acid tolerance, and thermostability. Gas chromatography-mass spectrometry(GC-MS) and enzymatic analysis elucidated a distinct degradation pathway involving monooxygenase and catechol 1,2-dioxygenase, leading to complete mineralization. Unlike previous halotolerant bacteria studies focusing on general organics, this work provides deep mechanistic insights into aromatic metabolism under high salinity and acidic conditions. Applied to real SGFFF, the strain achieved 37–47 % TOC removal, showcasing significant potential for enhancing bio-treatment efficacy in hypersaline wastewater.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"225 ","pages":"Article 109944"},"PeriodicalIF":3.7,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of microcarriers as a 3D platform for differentiation of iPSC into pancreatic islet-like clusters 微载体作为诱导多能干细胞分化为胰岛样细胞簇的三维平台的评价

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-09-25 DOI: 10.1016/j.bej.2025.109941

Nyna Kawles , Danton Freire-Flores , Pablo Caviedes , Juan A. Asenjo , Barbara A. Andrews

{"title":"Evaluation of microcarriers as a 3D platform for differentiation of iPSC into pancreatic islet-like clusters","authors":"Nyna Kawles , Danton Freire-Flores , Pablo Caviedes , Juan A. Asenjo , Barbara A. Andrews","doi":"10.1016/j.bej.2025.109941","DOIUrl":"10.1016/j.bej.2025.109941","url":null,"abstract":"<div><div>Scalable production of stem cell-derived β-like cells is essential for advancing iPSC-based therapies for type 1 diabetes. Microcarriers offer a promising strategy for bioprocess intensification due to their high surface-to-volume ratio and compatibility with suspension bioreactors. This study evaluated whether microcarriers can support the direct differentiation of induced pluripotent stem cells (iPSC) into β-like cells under dynamic conditions. iPSC were cultured on Cytodex-1 microcarriers in spinner flasks and differentiated using two protocols: Hogrebe et al. and Velazco-Cruz et al. Differentiation efficiency and marker expression were assessed via flow cytometry and immunohistochemistry. Due to poor differentiation outcomes, an alternative approach was tested: iPSC were first differentiated into pancreatic progenitor cells (PPCs) in 2D, enriched for GP2<sup>+</sup> cells via MACS, and then expanded on microcarriers. Neither protocol induced β-cell maturation on microcarriers, as shown by poor cell adhesion and absence of key markers. However, microcarriers supported robust expansion of GP2<sup>+</sup> PPCs, with higher proliferation rates and preservation of PDX1<sup>+</sup> and NKX6.1<sup>+</sup> profiles compared to 2D culture. This expansion stage provides a critical intermediate for scalable α and β-like cell production, facilitating large-scale PPC generation for subsequent aggregation and terminal differentiation—though this was not tested in this study. This strategy offers a scalable upstream solution for producing high-quality precursor cells for islet-like cluster formation in future therapeutic applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"225 ","pages":"Article 109941"},"PeriodicalIF":3.7,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance of anoxic and aerobic flocs in anoxic/aerobic-membrane bioreactor (A/O–MBR) for the treatment of printing and dyeing wastewater (PDW) 厌氧/好氧-膜生物反应器（A/ O-MBR）处理印染废水（PDW）的厌氧和好氧絮凝体性能

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-09-25 DOI: 10.1016/j.bej.2025.109943

Chunkai Huang , Han Zhang , Jincan Huang , Lu Zhang , Siqi Tong , Feng Wang , Guangbing Liu , Xuemin Yu , Haibo Xu , Weijing Liu

{"title":"Performance of anoxic and aerobic flocs in anoxic/aerobic-membrane bioreactor (A/O–MBR) for the treatment of printing and dyeing wastewater (PDW)","authors":"Chunkai Huang , Han Zhang , Jincan Huang , Lu Zhang , Siqi Tong , Feng Wang , Guangbing Liu , Xuemin Yu , Haibo Xu , Weijing Liu","doi":"10.1016/j.bej.2025.109943","DOIUrl":"10.1016/j.bej.2025.109943","url":null,"abstract":"<div><div>This study compared and elucidated the performance and mechanisms of aerobic and anoxic flocs in anoxic/aerobic-membrane bioreactor (A/O–MBR) for the printing and dyeing wastewater (PDW) treatment. The batch experiments were conducted with aerobic and anoxic flocs from our continuous laboratory-scale A/O–MBR for the PDW remediation. A distinct discrepancy between aerobic and anoxic activated sludge flocs for chemical oxygen demand (COD) and adsorbable organic halogens (AOX) removal was demonstrated. Specifically, the aerobic flocs exhibited considerable higher COD removal efficiencies (O1 59.0 %, O2 76.4 %) than the anoxic flocs (A1 56.5 %, A2 61.0 %) owing to the stronger organic biodegradability of aerobic respiration, while anoxic flocs exhibited greater performance on the AOX removal (A1 87.8 %) than aerobic flocs (O1 76.8 %) after 12 h biodegradation due to the greater abundance of AOX degradation relevant gene in anoxic flocs. Meanwhile, the study also revealed that the biodegradation was the principal mechanism for COD removal in PDW, whereas, both biodegradation and biosorption contributed to the AOX removal. This study provides valuable insights into the upgrading and reconstruction of A/O process in PDW treatment plants.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"225 ","pages":"Article 109943"},"PeriodicalIF":3.7,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling meets metabolic engineering: The iPichia consensus model as basis for metabolic studies in Komagataella phaffii 建模符合代谢工程：iPichia共识模型作为Komagataella phaffii代谢研究的基础

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-09-25 DOI: 10.1016/j.bej.2025.109940

Pınar Kocabaş

{"title":"Modeling meets metabolic engineering: The iPichia consensus model as basis for metabolic studies in Komagataella phaffii","authors":"Pınar Kocabaş","doi":"10.1016/j.bej.2025.109940","DOIUrl":"10.1016/j.bej.2025.109940","url":null,"abstract":"<div><div><em>Komagataella phaffii</em> (syn. <em>Pichia pastoris</em>) has become one of the most commonly employed hosts for recombinant protein expression, with methanol-utilizing (Mut⁺) strains being used to produce more than 400 different proteins. The availability of fully sequenced and functionally annotated genomes has greatly facilitated systems biology studies and enabled the generation of genome-scale metabolic models (GEMs) for this species. In this study, two previously published GEMs (Kp.1.0 and <em>i</em>AUKM) were systematically merged in order to build a unified consensus model, referred to as <em>i</em>Pichia, that offers a more complete description of <em>K. phaffii</em> metabolism for the first time. <em>i</em>Pichia GEM was then extended by introducing enzyme capacity limitations through the GECKO 3.0 framework, yielding an enzyme-constrained genome-scale model (ecPichia GEM) for <em>Komagataella phaffii</em>. The resulting ecPichia GEM is the first enzyme constraint genome scale metabolic model for <em>Komagataella phaffii.</em> The predictive performance of ecPichia GEM for growth was evaluated using data from the literature. Gene essentiality analyses were carried with <em>i</em>Pichia compared to protein-constrained ecPichia GEM, Kp.1.0 and <em>i</em>AUKM GEMs. Thereafter, the model was applied to uncover promising targets for metabolic engineering aimed at producing bisabolene — an industrially relevant sesquiterpene used in both biofuel and fragrance applications. Overall, the results underline the effectiveness of enzyme-constrained metabolic modeling as a tool to support rational strain development in the field of industrial biotechnology.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"225 ","pages":"Article 109940"},"PeriodicalIF":3.7,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhance protein secretion pathway and energy metabolism to improved protein activity in recombinant Pichia pastoris 改善重组毕赤酵母的蛋白质分泌途径和能量代谢，提高蛋白质活性

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-09-23 DOI: 10.1016/j.bej.2025.109935

Hao Shi , Hao Chen , Tianwei Tan

{"title":"Enhance protein secretion pathway and energy metabolism to improved protein activity in recombinant Pichia pastoris","authors":"Hao Shi , Hao Chen , Tianwei Tan","doi":"10.1016/j.bej.2025.109935","DOIUrl":"10.1016/j.bej.2025.109935","url":null,"abstract":"<div><div>Methanol, as an inexpensive and clean energy source in the context of carbon neutrality and the methylotrophic yeast <em>Pichia pastoris</em> offers a unique pathway for methanol utilization. This study engineered the secretory pathway in <em>Pichia pastoris</em> GS115 to optimize the methanol-driven production of <em>Penicillium amazonicum</em>-derived glucose oxidase (GOX). Initially, high-copy α-SP was identified as the dominant expression strain from the six signal peptides. Subsequently, systematic overexpression of secretory pathway-related factors revealed that signal α-SP-mediated ER membrane targeting (SEC12) and its synergistic interplay with ER-resident folding (ERO1) machinery critically determined secretion capacity and methanol bioconversion efficiency. PPG-ERO1-SEC12 achieved a yield of 198.73 U/mL in shake-flask cultures. An imbalance between central carbon metabolism and energy level was found, which revealed low energy utilization efficiency and prolonged oxidative stress. Key energy metabolism genes (<em>noxE, FDH1, PYK1,</em> and <em>IDH1</em>) were overexpressed and enhanced the activities of key enzymes (<em>AOX, FLD, FDH, CAT,</em> and <em>IDH)</em> in the energy metabolism pathway, but also increased protein activity by 3.2-fold. Finally, this approach achieved a glucose oxidase yield of 2604.20 U/mL (5.09 g/L protein titer) in 5-L batch fermentation, with a specific activity of 511.63 U/mg and > 95 % purity. This groundbreaking strategy not only offers valuable technical insights for industrial applications but also has the potential to reduce costs and enhance efficiency.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"225 ","pages":"Article 109935"},"PeriodicalIF":3.7,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0