Efficient benzaldehyde degradation by Barnettozyma hawaiiensis and its application in shale gas fracturing flowback fluid treatment: Identification, performance, and mechanisms

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2025-09-26 DOI:10.1016/j.bej.2025.109944

Hongmei Yang , Yingxue Geng , Wenshi Gou , Ping Yang

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引用次数: 0

Abstract

This study isolated a novel halotolerant and acid-tolerant yeast, Barnettozyma hawaiiensis, from raw shale gas fracturing flowback fluid (SGFFF), demonstrating high-efficiency degradation of benzaldehyde—a representative toluene-derived aromatic intermediate. Under optimized conditions (pH 5, 20 g/L NaCl, 35°C), it achieved 98.28 % benzaldehyde and 74.76 % total organic carbon(TOC) removal within 24 h. Whole-genome sequencing revealed abundant genes related to organic degradation, salt/acid tolerance, and thermostability. Gas chromatography-mass spectrometry(GC-MS) and enzymatic analysis elucidated a distinct degradation pathway involving monooxygenase and catechol 1,2-dioxygenase, leading to complete mineralization. Unlike previous halotolerant bacteria studies focusing on general organics, this work provides deep mechanistic insights into aromatic metabolism under high salinity and acidic conditions. Applied to real SGFFF, the strain achieved 37–47 % TOC removal, showcasing significant potential for enhancing bio-treatment efficacy in hypersaline wastewater.

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夏威夷巴尼托菌高效降解苯甲醛及其在页岩气压裂返排液中的应用：鉴定、性能及机理

本研究从原始页岩气压裂返排液（SGFFF）中分离出一种新型耐盐耐酸酵母——夏威夷巴纳特酵母菌（Barnettozyma hawaiiensis），证明了它对苯甲醛（一种典型的甲苯衍生芳香中间体）的高效降解。在最佳条件（pH 5, 20 g/L NaCl, 35℃）下，24 h内苯甲醛去除率为98.28 %，总有机碳去除率为74.76 %。全基因组测序揭示了大量与有机降解、盐/酸耐受性和热稳定性相关的基因。气相色谱-质谱（GC-MS）和酶分析揭示了一种独特的降解途径，涉及单加氧酶和儿茶酚1,2-双加氧酶，导致完全矿化。与以往针对一般有机物的耐盐细菌研究不同，这项工作为高盐度和酸性条件下芳香代谢提供了深入的机制见解。应用于实际的SGFFF，菌株TOC去除率达到37-47 %，显示出提高高盐废水生物处理效果的巨大潜力。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.