Transcriptomic analysis of the effects of nutritional conditions on Rhodosporidium toruloides lipid production

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2025-09-26 DOI:10.1016/j.bej.2025.109945

Yulong Yang , Zi Ye , Minrui Guo , Guogang Chen

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引用次数: 0

Abstract

Rhodosporidium toruloides is an excellent oleaginous yeast that produces lipids that can be used for energy production and chemical industry, with enormous application potential and economic value. The lipid production capacity of R. toruloides is affected by many factors, among which carbon (C) source, nitrogen (N) source, and C/N ratio are the key influencing factors. Therefore, in this study, the growth and lipid synthesis ability of R. toruloides were evaluated under different nutritional conditions, and transcriptome sequencing was performed. The results showed that the T2 experimental group (sucrose, C/N = 200) exhibited the highest lipid production capacity, with a lipid yield of 6.43 ± 0.26 g/L and a lipid content of 67.32 %, compared with the CK control group (glucose, C/N = 20) and the T1 experimental group (sucrose, C/N = 20). RNA-Seq analysis was conducted on all three groups to investigate the underlying molecular mechanisms. Enrichment analysis showed that N limitation up-regulated the expression of genes related to triglyceride synthesis and fatty acid synthesis, down-regulated the expression of genes related to glycolysis, phosphopentose pathway and tricarboxylic acid cycle, and inhibited cell proliferation, leading to enhanced carbon flow to lipid synthesis. This study explored the metabolic relationship between nitrogen limitation and lipid synthesis in R. toruloides, providing insights for improving lipid production and its industrial application.

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营养条件对环形红孢子虫脂质产生影响的转录组学分析

toruloides红孢子虫是一种优良的产油酵母，可生产用于能源生产和化学工业的脂质，具有巨大的应用潜力和经济价值。影响圆叶藻产脂能力的因素很多，其中碳(C)源、氮(N)源和碳氮比是关键影响因素。因此，本研究在不同的营养条件下，评估了圆尾圆叶藻的生长和脂质合成能力，并进行了转录组测序。结果表明,T2实验组(蔗糖、C / N = 200)表现出最高的脂质生产能力,脂质产量为6.43 ±0.26  g / L和67.32 %的脂质含量,与CK相比对照组(葡萄糖,C / N = 20)和T1实验组(蔗糖、C / N = 20)。对所有三组进行RNA-Seq分析以研究潜在的分子机制。富集分析表明，限氮可上调甘油三酯合成和脂肪酸合成相关基因的表达，下调糖酵解、磷酸戊糖途径和三羧酸循环相关基因的表达，抑制细胞增殖，导致脂质合成碳流增强。本研究探讨了圆叶蝉氮素限制与脂质合成之间的代谢关系，为提高脂质产量及其工业应用提供了新的思路。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.