低碳模式下生物铁污泥驱动氨氧化耦合硝酸盐还原：性能与机制

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2025-10-03 DOI:10.1016/j.bej.2025.109951

Lihong Zhang , Hongrong Zhao

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引用次数: 0

摘要

Fe(III)-还原耦合NH4+-N氧化（Feammox）和Fe(II)-氧化耦合NOx -N还原（NDFO）是近年来新发现的具有成本效益的生物脱氮技术。为确定低碳模式下的脱氮效率，建立了AOCNR体系，并对其在零碳（C/N = 0）和低碳（C/N = 0.5）条件下的脱氮性能和机理进行了研究。结果表明，低碳条件下TN去除率显著提高，最大去除率为38.24 mg/L，其中NH4+-N氧化率为18.65 mg/L， NO3—N还原率为87.9% %。批量实验证实，适度增加NO3—N可促进NH4+-N的氧化，过量则抑制NO3—N的还原。外部补铁（30 g/L）明显改善了NO₃⁻-N的去除，但可能导致NH4+-N的积累。同位素示踪显示Feammox和NDFO之间有协同作用，NH₄⁺-N氧化的主要产物是NO₂-N。此外，在AOCNR系统中检测到与Fe(III)/Fe（II）循环驱动脱氮相关的优势功能属（如Thauera， Ferruginibacter）。

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Ammonia oxidation coupled nitrate reduction driven by bio-iron sludge in low-carbon mode: Performance and mechanisms

Fe(III)-reduction coupled NH₄⁺-N oxidation (Feammox) and Fe(II)-oxidation coupled NO_x^--N reduction (NDFO) were newly discovered, cost-effective biological nitrogen removal technologies in recent years. To determine the nitrogen removal efficiency under low-carbon mode, the AOCNR system was established, and its nitrogen removal performance and mechanisms were investigated under both zero-carbon (C/N = 0) and low-carbon (C/N = 0.5) conditions. The results demonstrated that TN removal efficiency significantly improved under low-carbon conditions, with a maximum removal capacity of 38.24 mg/L, including 18.65 mg/L of NH₄⁺-N oxidation and 87.9 % NO₃^--N reduction rate. Batch experiments confirmed that moderate increase in NO₃^--N could enhance NH₄⁺-N oxidation, while excessive concentrations inhibited NO₃^--N reduction. External iron supplementation (30 g/L) markedly improved NO₃⁻-N removal but might lead to NH₄⁺-N accumulation. Isotope tracing revealed the synergistic effect between Feammox and NDFO, and the main product of NH₄⁺-N oxidation was NO₂⁻-N. In addition, dominant functional genera (e.g., Thauera, Ferruginibacter) related to Fe(III)/Fe(II) cycle-driven nitrogen removal were detected in the AOCNR system.

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.