Biochemical Engineering Journal最新文献_第2页

Oxygen uptake rate analysis to evaluate the impact of hydrodynamic stress on the growth of the avian cell line DuckCelt®-T17 通过摄氧量分析评估流体动力压力对禽类细胞系 DuckCelt®-T17 生长的影响

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-05 DOI: 10.1016/j.bej.2024.109520

{"title":"Oxygen uptake rate analysis to evaluate the impact of hydrodynamic stress on the growth of the avian cell line DuckCelt®-T17","authors":"","doi":"10.1016/j.bej.2024.109520","DOIUrl":"10.1016/j.bej.2024.109520","url":null,"abstract":"<div><div>Scale-up of bioprocesses involving animal cell culture is hampered by the sensitivity of the cells to hydrodynamic stress, either from agitation or bubble bursting. Here, the hydrodynamic stress experienced by a recent cell line, the DuckCelt®-T17 avian cells, previously used for viral vaccine production, is investigated in shake flasks and in a 3 L bioreactor. Cell stress was assessed by monitoring the dissolved oxygen in the culture medium, which depends on Oxygen Transfer Rate (OTR) and Oxygen Uptake Rate (OUR) during cultivation. Classical parameters such as the maximum growth rate (<em>µ</em><sub>max</sub>) and metabolite profiles were also determined. A dynamic model able to predict nutrient consumption, metabolic waste production, viable cell number and OUR was also developed and validated from the data measured in shake flasks. The experiments performed in the stirred tank bioreactor (STBR) show that OUR depended on both the cell growth phase and the stirring conditions. The oxygen consumption of the cells during the exponential growth phase (where there were no nutrient and O<sub>2</sub> limitations) was significantly altered at average and maximum shear rates above 70 and 840 s<sup>−1</sup>, respectively, indicating highly shear-sensitive cells. OUR is a suitable tool to identify the hydrodynamic conditions for robust cell growth. The scale-up criteria to be favored for the DuckCelt®-T17 cell culture in STBRs would be the shear and/or the tip’s speed.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streamlining process development and scale-up: Risk assessment to reduce workload in primary protein recovery 简化工艺开发和放大：风险评估以减少初级蛋白质回收的工作量

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-05 DOI: 10.1016/j.bej.2024.109513

{"title":"Streamlining process development and scale-up: Risk assessment to reduce workload in primary protein recovery","authors":"","doi":"10.1016/j.bej.2024.109513","DOIUrl":"10.1016/j.bej.2024.109513","url":null,"abstract":"<div><div>Risk assessment is an integral aspect of the Quality-by-Design strategy to identify potential obstacles at every stage of biopharmaceutical production, from process development to tech transfer. We explored flow process chart, root cause analysis, and failure mode and effects analysis, to assess the scale-up of bacterial cell disruption and its influence on centrifugation and filtration steps. The Ishikawa diagram suggests that data on the impact of homogenizer valve design on product release, impurity profile, particle size distribution, viscosity, and dsDNA fragment size are missing which were collected experimentally for this study. Cell lysates from micro-, lab- and pilot scales cell disruption were analyzed for the above-mentioned parameters. Process parameters affecting these output parameters were identified on each individual scale. Cell disruption on the micro scale was performed in a bead mill. High pressure homogenization was used on lab- and pilot scales. Cell disintegration by bead milling delivers homogenates of product and impurity content comparable to those on bench scale but with 3-fold higher viscosity and significantly larger dsDNA fragments, 8.0 instead of 1.0 kbp, respectively. Miniaturized pressure flow curves identified dsDNA fragment sizes as critical for filter performance during clarification. Combining risk assessment, micro scale cell disintegration and bench scale pressure flow curves allows for selective and efficient process development, and scale up for primary recovery steps.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A circular economy approach for utilization of tannery fleshing hydrolysate and kitchen wastes into organic fertilizer through enzymatic decomposition 通过酶分解将制革肉水解物和厨房废物利用为有机肥料的循环经济方法

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-02 DOI: 10.1016/j.bej.2024.109519

{"title":"A circular economy approach for utilization of tannery fleshing hydrolysate and kitchen wastes into organic fertilizer through enzymatic decomposition","authors":"","doi":"10.1016/j.bej.2024.109519","DOIUrl":"10.1016/j.bej.2024.109519","url":null,"abstract":"<div><div>This study presents a sustainable method for converting tannery fleshing waste (FH) into organic fertilizer using enzymatic decomposition with crude protease. After extracting fat from the enzymatic hydrolysis, the enzyme-rich residue was mixed with dried kitchen waste (KW) and allowed to decompose for 45 days, producing nutrient-rich fertilizer. FT-IR spectroscopy confirmed the presence of important functional groups, including hydroxyl, aliphatic hydrocarbons, esters, and amide-I linkages. The organic fertilizers had higher nutrient content, with nitrogen (1.08-1.67%), phosphorus (0.78–0.98 %), potassium (0.1–0.76 %), and magnesium (239–259.5 ppm) which is higher than commercial fertilizers. FESEM-EDX analysis revealed a dense, porous structure with a high surface concentration of calcium, which enhances nutrient release in the soil. Dissolution tests showed that nutrients from the organic fertilizer were released gradually over 36 hours, whereas commercial NPK fertilizers released nutrients within 150 minutes in simulated soil-water conditions. Field trials with a Randomized Complete Block Design (RCBD) demonstrated improved growth in Malabar spinach, particularly with fertilizer sample S-5, which had an optimal flesh to kitchen waste (FH to KW) ratio of 1:10. Although S-5 had a lower nitrogen content (1.08 %), it contained higher levels of phosphorus (0.98 %), potassium (0.765 %), and magnesium (259.5 ppm), contributing to enhanced plant growth. The organic fertilizer resulted in a shoot length of 38.8 ± 2.0 cm, root length of 16.33 cm, 31 ± 3 leaves, and 95.12 % dry matter of the plant. Heavy metal analysis of the plant confirmed that levels of chromium (Cr), iron (Fe), nickel (Ni), cadmium (Cd), copper (Cu), and lead (Pb) were within WHO safety limits. Phytotoxicity tests of the fertilizer also showed no negative impact on Malabar spinach seed germination.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategies for enhanced acetone-butanol-ethanol production by Clostridium beijerinckii Y10 from glucose and xylose and exploration of its physiological mechanisms 增强贝氏梭菌 Y10 利用葡萄糖和木糖生产丙酮-丁醇-乙醇的策略及其生理机制探索

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-02 DOI: 10.1016/j.bej.2024.109518

引用次数: 0

Long lasting degradation of all alkanes in soil by Pseudomonas activated after Fenton pre-oxidation 经 Fenton 预氧化处理的假单胞菌对土壤中所有烷烃的长效降解作用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-02 DOI: 10.1016/j.bej.2024.109511

{"title":"Long lasting degradation of all alkanes in soil by Pseudomonas activated after Fenton pre-oxidation","authors":"","doi":"10.1016/j.bej.2024.109511","DOIUrl":"10.1016/j.bej.2024.109511","url":null,"abstract":"<div><div>This study investigated the function and mechanism of Fenton pre-oxidation on the long lasting degradation of all alkanes in soil contaminated by petroleum. The findings demonstrated that the biological removal amount of all alkanes in the respiratory regulation group reached 4083.46 mg/kg, which was twice that of the non-regulation group, and the removal amount gradually increased in the four stages of bioremediation. In addition, the removal amount of all alkanes in the non-regulated group did not change much and showed a downward trend, indicating that long lasting degradation of all alkanes could be achieved by the respiratory regulation group, and the biodegradation cycle was saved by 251 days compared with the non-regulated group. Furthermore, the total number of bacteria in the respiratory regulation group (6.73 log CFU/g) was significantly higher than that in the non-regulation group (2.25 log CFU/g). <em>Pseudomonas</em> became the dominant genus in the respiratory regulation group with an average relative abundance of 32.17 %. In the respiratory regulation group, a large amount of ammonia nitrogen (1703.62 mg/kg) was consumed during the degradation process, which stimulated the tricarboxylic acid cycle respiratory metabolism process of <em>Pseudomonas</em> and accelerated the hydrocarbon conversion. This may be the reason why the long lasting degradation of all alkanes in soil could be achieved by the respiratory regulation group.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural biocide-assisted ultrasonic disinfection of wastewater effluent following a response surface methodology approach 采用响应面方法对废水进行天然杀菌剂辅助超声波消毒

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-01 DOI: 10.1016/j.bej.2024.109517

{"title":"Natural biocide-assisted ultrasonic disinfection of wastewater effluent following a response surface methodology approach","authors":"","doi":"10.1016/j.bej.2024.109517","DOIUrl":"10.1016/j.bej.2024.109517","url":null,"abstract":"<div><div>The increasing prevalence of multidrug-resistant (MDR) bacteria in wastewater poses a significant threat to public health and the environment, necessitating more effective and sustainable disinfection methods. Ozonation and chlorination frequently fall short of eliminating these bacteria and can create toxic byproducts. This study introduces a novel disinfection strategy that combines ultrasonication with tea tree oil to target MDR bacteria in residential wastewater treatment systems, aiming to provide an eco-friendly, efficient, and scalable solution. The method harnesses tea tree oil's natural biocidal properties alongside the physical effects of ultrasonication, particularly acoustic cavitation, to enhance bacterial inactivation. Temperature, biocide dosage, and ultrasonication power were the three main factors that were optimized using response surface methodology. The system achieved a 2.2–2.4 log CFU/mL reduction of total bacteria in secondary effluent within 30 min and complete disinfection of modified effluent inoculated with high-strength MDR bacteria (6-log CFU/mL) in 50 min. Optimal conditions were 698.4 Watt power, 1.234 µl/mL tea tree oil, and 20.64 °C. Nucleic acid release and respiratory chain dehydrogenase inhibition indicated bacterial cell membrane rupture. Regrowth tests showed long-term effectiveness, with no bacterial colonies after three days. Using a natural biocide, the hybrid technique reduces operational costs and time, thus having commercial and environmental benefits. The capacity to remove MDR bacteria makes it an attractive contender for large-scale wastewater treatment.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fermentative production of 3-hydroxypropionic acid by using metabolically engineered Klebsiella pneumoniae strains 利用代谢工程肺炎克雷伯菌株发酵生产 3-羟基丙酸

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-01 DOI: 10.1016/j.bej.2024.109516

{"title":"Fermentative production of 3-hydroxypropionic acid by using metabolically engineered Klebsiella pneumoniae strains","authors":"","doi":"10.1016/j.bej.2024.109516","DOIUrl":"10.1016/j.bej.2024.109516","url":null,"abstract":"<div><div>3-Hydroxypropionic acid (3-HP) is an industrially important platform chemical for super-absorbent or biodegradable polymers. Its production via biological methods is expected to be more competitive than chemical methods. <em>Klebsiella pneumoniae</em> is the most promising host due to its innate capabilities for 3-HP and vitamin-B12 production, ease of culture, and ease of engineering. In this study, step-by-step metabolic engineering and fermentation technologies were used to enhance the production of 3-HP. <em>K. pneumoniae</em>-derived <em>ydcW</em> gene was overexpressed using a plasmid after screening candidate genes. Major competing pathways encoded by <em>dhaT</em>, <em>yqhD</em>, <em>ldhA</em>, <em>glpK</em>, <em>poxB</em>, and <em>pta-ackA</em> were blocked. Additionally, it was demonstrated that simultaneous reinforcement of two native aldehyde dehydrogenase encoded by the <em>ydcW</em> gene preferring NADPH and the <em>puuC</em> gene preferring NADH, synergistically improved 3-HP production. Additional reinforcement of the <em>acs</em> gene to reduce acetate accumulation resulted in 93.7 g/L of 3-HP with a yield of 0.42 g/g·glycerol over a 72-h fed-batch fermentation. This performance is deemed sufficient for industrial applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced S-adenosyl-L-methionine synthesis in Saccharomyces cerevisiae using metabolic engineering strategies 利用代谢工程策略提高酿酒酵母中 S-腺苷-L-蛋氨酸的合成能力

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-09-28 DOI: 10.1016/j.bej.2024.109504

{"title":"Enhanced S-adenosyl-L-methionine synthesis in Saccharomyces cerevisiae using metabolic engineering strategies","authors":"","doi":"10.1016/j.bej.2024.109504","DOIUrl":"10.1016/j.bej.2024.109504","url":null,"abstract":"<div><div>S-adenosyl-L-methionine (SAM) plays pivotal roles in various physiological processes. With its increasing application in the treatment of diseases such as liver disease, depression, osteoarthritis and Alzheimer's, interest in SAM production aroused. Currently, <em>Saccharomyces cerevisiae</em> is the main industrial producer of SAM. With the surge in demand for SAM, improving the SAM biosynthesis is of importance. In this study, a multimodule engineering strategy was employed to improving SAM production: 1) Enhancing the gene expression of the sulfur assimilation pathway; 2) Strengthening the metabolic flux of the SAM synthesis pathway; 3) Weakening the SAM degradation pathway; 4) Increasing ATP supply. The resulting engineered mutant SC06 (<em>S. cerevisiae</em> CEN.PK2–1CΔ<em>gal</em>80<em>∷T</em><sub><em>cyc1</em></sub>-<em>sam2-P</em><sub><em>gal1</em></sub><em>-P</em><sub><em>gal10</em></sub><em>-met14-T</em><sub><em>adh1</em></sub>, Δ<em>lsc2∷T</em><sub><em>cyc1</em></sub>-<em>hom6-P</em><sub><em>gal1</em></sub><em>-P</em><sub><em>gal10</em></sub><em>-met6-T</em><sub><em>adh1</em></sub>, Δ<em>sah1</em>Δ<em>mls1</em>) displayed the highest SAM titer of 240.86 mg/L, which was 10.22-fold increase compared with the original strain. With optimized conditions, the SAM titer of mutant SC06 in shake flask fermentation reached 473.02 mg/L with a specific yield of 127.18 mg/g dry cell weight (DCW). In a 5 L fermenter with fed-batch fermentation, the maximal SAM yield of mutant SC06 reached 1.25 g/L with a specific yield of 166.67 mg/g DCW after 58 h cultivation. Therefore, the established metabolic engineering strategies displayed promising efficiency in improving the SAM productivity of <em>S. cerevisiae</em> CEN.PK2–1C, which may provide a useful tool for the improvement of SAM-producing strains.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced oxidation enhanced microbial electrolysis cell coupled with anaerobic digestion: A novel approach to coal gasification wastewater treatment 高级氧化强化微生物电解池与厌氧消化相结合：煤气化废水处理的新方法

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-09-27 DOI: 10.1016/j.bej.2024.109512

{"title":"Advanced oxidation enhanced microbial electrolysis cell coupled with anaerobic digestion: A novel approach to coal gasification wastewater treatment","authors":"","doi":"10.1016/j.bej.2024.109512","DOIUrl":"10.1016/j.bej.2024.109512","url":null,"abstract":"<div><div>Coal gasification wastewater (CGW) contains a range of refractory and toxic organic pollutants with low biodegradability and significant biological toxicity. This study synthesized a hemin-graphene (H-graphene) catalyst for permonosulfate (PMS) activation. The MEC-AD (Microbial electrolysis cell- Anaerobic digestion) reactor (K1) was designated as the control group. The experimental groups were the MEC-AD reactor (K2) with PMS and H-graphene, and the MEC-AD reactor (K3) with advanced oxidation as a synthetic CGW pretreatment. The results demonstrated that the COD removal rates of K1, K2 and K3 reactors were 76.7 %, 79.5 % and 87.4 %, while the total phenol removal rates were 74.1 %, 90.1 % and 100 %, respectively. Quinoline and indole were removed at rates greater than 90 % in the microbial electrolytic cell reactors K2 and K3, and 100 % in the K3 reactor. In K2 and K3, there was a considerable decrease in the abundance of <em>unclassified _ f _ Alcaligenaceae, Arenimonas</em> and <em>unclassified _ f _ Gracilibacteraceae</em> as compared to K1. The abundance of <em>unclassified _ p _ Zixibacteria, Candidatus _ Caldatribacterium, unclassified _ c _ JS1 and JGI-0000079-D21,</em> which are responsible for promoting the anaerobic degradation of long-chain fatty acids and anaerobic fermentation of acid production increased.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cutting-edge computational approaches in enzyme design and activity enhancement 酶设计和活性增强的前沿计算方法

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-09-26 DOI: 10.1016/j.bej.2024.109510

引用次数: 0