Biochemical Engineering Journal最新文献_第2页

Rapid detection of Staphylococcus aureus in animal-derived foods using aptamer-based fluorescent lateral flow biosensors 基于适配体的荧光侧流生物传感器快速检测动物源性食品中的金黄色葡萄球菌

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-11 DOI: 10.1016/j.bej.2025.109756

Shuxian Zhou , Yinghui Wang , Haifang Wang , Shouyi Dou , Mengyue Liu , Haowei Dong , Zhengtao Li , Donghan Li , Jingjing Liu , Xia Sun , Yemin Guo , Ibrahim A. Darwish , Yanfang Wu

{"title":"Rapid detection of Staphylococcus aureus in animal-derived foods using aptamer-based fluorescent lateral flow biosensors","authors":"Shuxian Zhou , Yinghui Wang , Haifang Wang , Shouyi Dou , Mengyue Liu , Haowei Dong , Zhengtao Li , Donghan Li , Jingjing Liu , Xia Sun , Yemin Guo , Ibrahim A. Darwish , Yanfang Wu","doi":"10.1016/j.bej.2025.109756","DOIUrl":"10.1016/j.bej.2025.109756","url":null,"abstract":"<div><div><em>Staphylococcus aureus</em> (<em>S. aureus</em>), commonly found in animal-derived foods, poses a significant threat to food safety. Traditional detection methods often suffer from limited sensitivity and selectivity, are time-consuming, and involve complex procedures. In this study, fluorescent lateral flow biosensors (LFB) based on aptamer recognition were developed for the rapid and cost-effective detection of <em>S. aureus</em>. The method utilizes a Cy5-labeled aptamer (Cy5-Apt) as a signal recognition molecule. Detection is achieved through the competitive binding of <em>S. aureus</em> and a complementary strand to the aptamer, enabling the fluorescence intensity measurement on the LFB within 10 min. Key factors including the Cy5-Apt concentration, the length of the linker arm, the position and length of the complementary strand to the aptamer, BSA concentration, and buffer composition were optimized. The method showed good linear response for the quantitative detection of <em>S. aureus</em> in the concentration range of 2.8 × 10<sup>1</sup> to 2.8 × 10<sup>7</sup> CFU mL<sup>−1</sup>, and the limit of detection (LOD) was 1.65 CFU mL<sup>−1</sup>. Application tests in milk and chicken meat confirmed the selectivity and sensitivity of this enzyme- and antibody-free approach. This aptamer-based method offers a promising solution for low-cost, rapid, and efficient detection of <em>S. aureus</em> in food safety applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109756"},"PeriodicalIF":3.7,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel high-temperature Kluyveromyces marxianus as a microbial cell factory host for sesquiterpene production 一种新型高温马氏克鲁维菌作为生产倍半萜的微生物细胞工厂宿主

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-11 DOI: 10.1016/j.bej.2025.109739

Min Zhang , Yongfu Ruan , Yi Hu , Yiying Huo , Meng Wang , Zhiwei Zhu , Yunming Fang

{"title":"A novel high-temperature Kluyveromyces marxianus as a microbial cell factory host for sesquiterpene production","authors":"Min Zhang , Yongfu Ruan , Yi Hu , Yiying Huo , Meng Wang , Zhiwei Zhu , Yunming Fang","doi":"10.1016/j.bej.2025.109739","DOIUrl":"10.1016/j.bej.2025.109739","url":null,"abstract":"<div><div>Artemisinin, a vital antimalarial agent, is traditionally extracted from <em>Artemisia annua</em> L. by plant-based extraction. The microbial biosynthesis of the artemisinin precursor, amorpha-4,11-diene (AD), followed by its chemical conversion into artemisinin provides a sustainable solution to meet the demand for artemisinin while overcoming the limitations of plant-based extraction. <em>Kluyveromyces marxianus</em> is a promising host for microbial cell factories owing to its robust thermotolerance, rapid growth kinetics, and ability to utilize a wide range of carbon sources. Herein, genetic modification tools for <em>K. marxianus</em>, including transformation methods and expression vectors, were identified and optimized, while the sesquiterpene biosynthetic capacity of <em>K. marxianus</em> was preliminarily evaluated. To enhance the precursor metabolite supply, eight key genes from the endogenous mevalonate pathway were overexpressed within the <em>K. marxianus</em> genome. A dual co-expression strategy, involving both extrachromosomal and chromosomally integrated expression of amorpha-4,11-diene synthase (<em>ADS</em>) was employed, followed by high-temperature fermentation to optimize production. Finally, an engineered <em>K. marxianus</em> strain was developed for the first time, capable of stable, efficient, and cost-effective production of AD, with a titer of 66.78 mg/L, a 113-fold increase compared with the wild-type strain. This engineered strain serves as a robust chassis and a valuable reference for the production and study of other terpenoids, laying the groundwork for further exploration of <em>K. marxianus</em> characteristics and the biosynthesis of AD.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109739"},"PeriodicalIF":3.7,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solar evaporation enabled household ethanol distillation and disinfection system for remote area 太阳能蒸发实现了偏远地区家用乙醇蒸馏和消毒系统

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-11 DOI: 10.1016/j.bej.2025.109757

Yijuan Wang , Ying Liao , Xiaojing Hou , Mingyang Shu , Liang Qian , Hao Li

{"title":"Solar evaporation enabled household ethanol distillation and disinfection system for remote area","authors":"Yijuan Wang , Ying Liao , Xiaojing Hou , Mingyang Shu , Liang Qian , Hao Li","doi":"10.1016/j.bej.2025.109757","DOIUrl":"10.1016/j.bej.2025.109757","url":null,"abstract":"<div><div>Ethanol concentrations of 70 %–90 % are commonly used to prevent healthcare-associated infections due to their effectiveness against various pathogens. However, high costs and transportation challenges limit the availability of commercial ethanol disinfectants, particularly in low- and middle-income countries. Local recycling of waste ethanol could address these issues, but large-scale implementation is hindered by the need for specialized equipment. Here, a small-scale, solar-powered ethanol recycling and disinfection device designed for household use was developed. Recycled melamine sponges, coated with polydopamine, serve as solar evaporators. The system achieves high evaporation rates (1.52–7.54 kg m<sup>−2</sup> h<sup>−1</sup>) for ethanol solutions ranging from 0 % to 100 %. Through multiple distillations, dilute ethanol can be concentrated to the required levels for medical disinfection. A closed-loop system recycles waste ethanol, and outdoor tests demonstrate its effectiveness in eliminating <em>Escherichia coli</em> and <em>Candida albicans</em> using solar-generated ethanol vapor. This work offers a sustainable solution for small-scale ethanol distillation and disinfection in remote areas.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109757"},"PeriodicalIF":3.7,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-dimensional and serum-free culture in fixed-bed bioreactor enhance exosome production by affecting the cytoskeleton through integrin β1 and RAC1 固定床生物反应器三维和无血清培养通过整合素β1和RAC1影响细胞骨架，促进外泌体的产生

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-11 DOI: 10.1016/j.bej.2025.109750

Chuanfu Huo , Yuanyuan Zhao , Meng Song , Yuzhe Guo , Shihao Li , Wensong Tan , Yan Zhou

{"title":"Three-dimensional and serum-free culture in fixed-bed bioreactor enhance exosome production by affecting the cytoskeleton through integrin β1 and RAC1","authors":"Chuanfu Huo , Yuanyuan Zhao , Meng Song , Yuzhe Guo , Shihao Li , Wensong Tan , Yan Zhou","doi":"10.1016/j.bej.2025.109750","DOIUrl":"10.1016/j.bej.2025.109750","url":null,"abstract":"<div><div>Exosomes are nanoscale vesicles containing a variety of bioactive factors, showing significant potential as cell-free therapeutic agents. However, the large clinical doses required for exosome administration highlight the need for a scalable serum-free production system to increase exosome yield. In this study, human umbilical cord-derived mesenchymal stem cells (hUCMSCs) were cultured in three-dimensional (3D) environments using polyethylene terephthalate (PET) fiber membranes. Two key pathways promoting exosome secretion and biogenesis through 3D culture were identified. Specifically, 3D culture reduced the expression of Integrin β1, leading to the decondensation of cortical actin, and increased the expression of RAC1, which promoted actin aggregation on the membranes of multivesicular bodies (MVBs). Building on this mechanistic understanding, we simulated the depolymerization/aggregation of the cytoskeleton in 3D culture and designed a serum-free medium optimized for exosome production. For large-scale exosome production, hUCMSCs were cultured in a fixed-bed bioreactor using PET fiber rolls as the fixed-bed layer with the developed serum-free medium. This setup increased exosome production per cell by 16-fold compared to 2D culture, yielding 2.6 × 10^14 exosome particles. The exosome products demonstrated enhanced <em>in vitro</em> angiogenesis, immunomodulation, and <em>in vivo</em> wound healing capabilities. This study provides valuable insights and strategic guidance for the serum-free, large-scale production of exosomes using bioreactors, with significant implications for therapeutic applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109750"},"PeriodicalIF":3.7,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Designing and fabrication of BIRC5-mRNA-based biosensor for early breast cancer diagnosis using a nanostructured (CeO2-Ru@AuNPs-CNTs)-lab-on-chip 基于birc5 - mrna的早期乳腺癌纳米传感器的设计与制造（CeO2-Ru@AuNPs-CNTs）

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-10 DOI: 10.1016/j.bej.2025.109754

Engy M. Madkour , Mohamed Abdel-Hakeem , Sahar A. Fadlallah , Sherif Abdelaziz Ibrahim , Rabeay Y.A. Hassan

{"title":"Designing and fabrication of BIRC5-mRNA-based biosensor for early breast cancer diagnosis using a nanostructured (CeO2-Ru@AuNPs-CNTs)-lab-on-chip","authors":"Engy M. Madkour , Mohamed Abdel-Hakeem , Sahar A. Fadlallah , Sherif Abdelaziz Ibrahim , Rabeay Y.A. Hassan","doi":"10.1016/j.bej.2025.109754","DOIUrl":"10.1016/j.bej.2025.109754","url":null,"abstract":"<div><div>Baculoviral Inhibitor of Apoptosis Repeat Containing 5 (BIRC5) gene, encoding the protein survivin, has garnered substantial research interest. Survivin is overexpressed in various cancers, including breast cancer, making BIRC5 a crucial biomarker for the early diagnosis and prognosis. Thus, a portable and disposable electrochemical biosensing system was designed and applied for the sensitive diagnosis of breast cancer targeting the BIRC5-mRNA. In this regard, gold nanoparticles assisted the formation of a self-assembling monolayer of the crosslinking chain (4-Aminothiophenol (ATP)) on the top of the nanocomposite layer that contains cerium oxide (CeO<sub>2</sub>)-Ruthenium (Ru)@multiwall carbon nanotubes (MWCNTs).Subsequently, the single stranded probe complementary to BIRC5-mRNA was chemically immobilized. Selectivity and sensitivity features of the newly designed BIRC5-mRNA-based biosensor were determined through a full assay optimization, whereas a detection limit of 0.4 ag/mL was successfully achieved. The newly developed nanostructured BIRC5 mRNA biosensor could be applied to provide a rapid and direct determination of the targeting BIRC5 mRNA in the patients’ blood samples without any additional labeling step that may be required in currently held RNA biosensors.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109754"},"PeriodicalIF":3.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased labyrinthopeptin A1 production is associated with changes in oxygen supply, density and elasticity of Actinomadura namibiensis pellets 迷宫肽A1生成的增加与纳米比亚放线菌颗粒的供氧、密度和弹性的变化有关

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-10 DOI: 10.1016/j.bej.2025.109747

Zuzanna Justyna Kozanecka , Jintian Liu , Qiyue Liu , Hannah Buch , Jona Gebauer , Detlev Rasch , Markus Böl , Rainer Krull

{"title":"Increased labyrinthopeptin A1 production is associated with changes in oxygen supply, density and elasticity of Actinomadura namibiensis pellets","authors":"Zuzanna Justyna Kozanecka , Jintian Liu , Qiyue Liu , Hannah Buch , Jona Gebauer , Detlev Rasch , Markus Böl , Rainer Krull","doi":"10.1016/j.bej.2025.109747","DOIUrl":"10.1016/j.bej.2025.109747","url":null,"abstract":"<div><div>Labyrinthopeptin A1, a promising broad-spectrum antiviral, is produced exclusively by the filamentous actinomycete <em>Actinomadura namibiensis</em>. In submerged cultures of <em>A. namibiensis</em>, supplementation with 50 mM ammonium sulfate resulted in a 6.3-fold increase in product formation, accompanied by enhanced glycerol consumption, lower dissolved oxygen tension, and changes in pellet morphology. This study aims to elucidate the underlying mechanisms of this bioprocess intensification method using novel tools such as oxygen microprofiling, plate-plate compression, and sedimentation experiments to investigate pellet characteristics possibly associated with product formation. Oxygen microprofiling revealed steeper profiles in salt-supplemented pellets, indicating heightened metabolic activity and potential oxygen limitation in pellet cores during exponential growth phase, which affects the subsequent secondary metabolite production. For the first time, pellet density was estimated using sedimentation experiments. While control pellets showed no density trends, salt-supplemented pellets became more porous over time, suggesting a link to the increased glycerol uptake during product formation. Additionally, the compression experiments showed greater increase in stiffness in salt-supplemented pellets over time, suggesting the development of stiffer structures within the hyphal network and increased pellet stability. This observation was previously inferred only through image analysis and cell dry weight concentration. Ultimately, the results presented here contribute to the development of numerical simulations and predictive models for the productivity and growth behavior of filamentous pellet cultures in the future.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109747"},"PeriodicalIF":3.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143828671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photochemical synthesis of polymer-stabilized silver nanoparticles for epinephrine sensing: Electrochemical, EG-FET and microcontroller based approaches 用于肾上腺素传感的聚合物稳定银纳米粒子的光化学合成：电化学，EG-FET和基于微控制器的方法

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-10 DOI: 10.1016/j.bej.2025.109755

Chandan Saha , Pooja Kumari , Lungelo Mgenge , Sarit K. Ghosh , Harishchandra Singh , Kaushik Mallick

{"title":"Photochemical synthesis of polymer-stabilized silver nanoparticles for epinephrine sensing: Electrochemical, EG-FET and microcontroller based approaches","authors":"Chandan Saha , Pooja Kumari , Lungelo Mgenge , Sarit K. Ghosh , Harishchandra Singh , Kaushik Mallick","doi":"10.1016/j.bej.2025.109755","DOIUrl":"10.1016/j.bej.2025.109755","url":null,"abstract":"<div><div>Epinephrine, a key catecholamine, regulates the sympathetic nervous system and plays a vital role in various physiological processes. This study presents various sensor applications for epinephrine detection, based on a polymer-stabilized silver nanoparticle composite catalyst, synthesized through a UV-mediated route. The metal-polymer composite system was characterized by transmission electron microscopy, X-ray diffraction, Raman and X-ray photoelectron spectroscopy techniques. The electrochemical redox behavior of epinephrine in the presence of the catalyst was studied using various voltametric and amperometric techniques. The electrocatalyst demonstrated high selectivity towards epinephrine detection in the presence of common biological interfering analytes like dopamine, uric acid and ascorbic acid. A sensing technique based on an extended gate field-effect transistor (EG-FET) was applied for epinephrine detection, achieving a limit of detection of 0.72 μM and a sensitivity of 0.049 μA.μM<sup>−1</sup>.cm<sup>−2</sup>. A microcontroller-based portable sensor was developed using an Arduino Uno R4 Wi-Fi module, enabling real-time monitoring and remote data transmission. The sensitivity and selectivity of the sensor make it a promising tool for on-site epinephrine detection in clinical and research applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109755"},"PeriodicalIF":3.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensitive indicator microorganisms and C,N-cycle processes in soil with different petroleum hydrocarbon pollution levels 不同石油烃污染程度土壤中敏感指示微生物及C、n循环过程

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-09 DOI: 10.1016/j.bej.2025.109752

Ting Zhang , Xuhong Zhang , Zeliang Liu , Yawen Ou , Xuhong Duan , Manli Wu

{"title":"Sensitive indicator microorganisms and C,N-cycle processes in soil with different petroleum hydrocarbon pollution levels","authors":"Ting Zhang , Xuhong Zhang , Zeliang Liu , Yawen Ou , Xuhong Duan , Manli Wu","doi":"10.1016/j.bej.2025.109752","DOIUrl":"10.1016/j.bej.2025.109752","url":null,"abstract":"<div><div>Total petroleum hydrocarbon(TPH) is toxic to soil microorganisms and humans and has become a serious public concern. This study employed chemical analysis, high-throughput sequencing, and KEGG database functional gene annotation to investigate microbial responses to TPH contamination and identify potential bioindicators for soil toxicity assessment. Our results demonstrated that TPH stress significantly altered soil microbial community stability and functional structure. In contaminated soils, the relative abundance of <em>Actinomycetes</em> increased by 14.03–25.06 %, while <em>Ascomycetes</em> and <em>Mortieres</em> increased by 0.85–2.2 % and 1.62–2.39 %, respectively, compared to uncontaminated soil. The Mantel test analysis identified that <em>Nocardioides, unclassified-Micrococcaceae, Streptomyces,</em> and <em>Gibberella</em> as robust bioindicators petroleum contamination(<em>p</em> < 0.05). Network analysis revealed that petroleum hydrocarbons enhanced microbial community complexity, with network edges increasing by 20–90 % in contaminated soils. PICRUSt2 functional prediction showed that TPH pollution altered microbial metabolic pathways, with bacterial metabolic processes and fungal biosynthesis processes dominating (>60 %) the total pathways. The abundance of nitrification genes involved in nitrogen transformation was positively correlated with soil oil content, denitrification, and nitrogen-fixing genes showed a negative correlation with TPH content. Expressions of functional genes involved in carbon photosynthesis fixation are inhibited in oil-contaminated soil with the hydroxypropanoate-hydroxybutylate cycle (M00375) being the main carbon cycle pathway.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109752"},"PeriodicalIF":3.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of different influent carbon-to-phosphorus ratios on phosphorus transformation, microbial community structure and metabolic functions in an upflow anaerobic sludge bed reactor 不同进水碳磷比对上流式厌氧污泥床反应器中磷转化、微生物群落结构和代谢功能的影响

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-08 DOI: 10.1016/j.bej.2025.109740

Jiahao Chen , Afeng Tao , Qiuhong Li , Zhengpeng Chen , Ziyi Wu , Xiaoyue Liu , Shenglong Chen , Yuxiang Lu , Yayuan Mo , Chengyuan Su

{"title":"Effects of different influent carbon-to-phosphorus ratios on phosphorus transformation, microbial community structure and metabolic functions in an upflow anaerobic sludge bed reactor","authors":"Jiahao Chen , Afeng Tao , Qiuhong Li , Zhengpeng Chen , Ziyi Wu , Xiaoyue Liu , Shenglong Chen , Yuxiang Lu , Yayuan Mo , Chengyuan Su","doi":"10.1016/j.bej.2025.109740","DOIUrl":"10.1016/j.bej.2025.109740","url":null,"abstract":"<div><div>Excessive phosphorus emissions can result in the eutrophication of water bodies, causing severe environmental damage as well as influencing the efficiency of water treatment equipment. The impacts of carbon/phosphorus ratios on performance and mechanism of the upflow anaerobic sludge bed reactor remain unclear. Henrie, the effects of different carbon/phosphorus ratios (i.e., 80:1, 40:1, and 20:1) on the transformation of phosphorus in the biological treatment process of an upflow anaerobic sludge blanket (UASB) reactor were studied. The results showed that phosphines are of great importance in the phosphate reduction process. After a stable operation, the phosphine reached the highest 81.91 mg/m<sup>3</sup> at a C/P ratio of 40:1. It was proved that the optimum operating condition of the reactor was carbon to phosphorus ratio of 40:1. Phosphate-reducing bacteria were present in the UASB reactor, and the relative abundance of <em>Clostridia</em> in the sludge was 1.90 % and 1.59 % when the C/P was 80:1 and 20:1, respectively. This implied that the low carbon to phosphorus ratio reduces the phosphorus-reducing microbial activity in the reactor. Lower C/P values could inhibit the uptake and use of P in the phosphonate transport system and the transport of phosphate in the cell by the microbial <em>Pst</em> system, impeding the mineralization of organophosphates. The study provides new insights into improving the efficiency of treating phosphorus-rich wastewater.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109740"},"PeriodicalIF":3.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ammonia nitrogen degradation metabolic pathway by the novel strain Paracoccus sp. TD-10 and its molecular mechanisms 新菌株副球菌sp. TD-10的氨氮降解代谢途径及其分子机制

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-08 DOI: 10.1016/j.bej.2025.109751

Ying Lei, Meimei Wan, Peiyang Zheng, Da Ao, Wenlong Yue, Zhiqiang Cai

{"title":"Ammonia nitrogen degradation metabolic pathway by the novel strain Paracoccus sp. TD-10 and its molecular mechanisms","authors":"Ying Lei, Meimei Wan, Peiyang Zheng, Da Ao, Wenlong Yue, Zhiqiang Cai","doi":"10.1016/j.bej.2025.109751","DOIUrl":"10.1016/j.bej.2025.109751","url":null,"abstract":"<div><div>Heterotrophic nitrification-aerobic denitrification（HN-AD） bacteria have attracted significant attention due to their high tolerance for ammonia nitrogen, efficient ammonia nitrogen degradation capabilities, and strong environmental adaptability in removing nitrogen contaminants from residential and industrial wastewater. This study aimed to investigate the ammonia nitrogen degradation characteristics of a novel HN-AD strain with superior ammonia nitrogen degradation capacity, providing a foundation for optimizing the ammonia nitrogen degradation processes in wastewater treatment facilities. The ammonia nitrogen degradation ability of <em>Paracoccus sp.</em> TD-10 was evaluated under specific conditions: pH 9.0, 28 ℃ and an initial concentration of 100.0 mg/L NH₄⁺, with sodium acetate serving as both the carbon and energy source. The results demonstrated a 100 % degradation rate under these conditions. Whole-genome analysis, functional gene identification, and nitrogen balance studies suggested a potential ammonia degradation pathway: NO₃⁻-N → NO₂⁻-N → NO → N₂O → N₂, and NH₄⁺-N → Gln → Glu. The exceptional ammonia nitrogen degradation efficiency of strain TD-10, combined with its ability to prevent the accumulation of nitrite and nitrate nitrogen, offers a solid theoretical basis for optimizing HN-AD bacteria in the sewage ammonia nitrogen degradation process.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109751"},"PeriodicalIF":3.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0