Biochemical Engineering Journal最新文献

{"title":"Enhanced continuous production of L-DOPA using tyrosinase immobilized on a tannic acid–iron(III) functionalized anodic aluminum oxide (AAO) membrane microreactor","authors":"Eunyoung Cho,&nbsp;Kyumin Park,&nbsp;Jung Heon Lee","doi":"10.1016/j.bej.2026.110150","DOIUrl":"10.1016/j.bej.2026.110150","url":null,"abstract":"<div><div>Microreactor-based biocatalytic processes offer significant advantages, including continuous operation, precise residence-time control, and enhanced process stability. In this study, a membrane-based microreactor incorporating a tannic acid–iron(III) (TA–Fe³⁺)–functionalized anodic aluminum oxide (AAO) membrane was developed for the continuous production of <span>L</span>-DOPA (3,4-dihydroxy-<span>L</span>-phenylalanine) using immobilized tyrosinase (Tyr). SEM and FTIR analyses confirmed that the TA–Fe³⁺ coating was deposited on the membrane surface and within its nanopores, providing a robust scaffold for enzyme attachment. Among the immobilization strategies investigated, enzyme adsorption–precipitation–crosslinking (EAPC) yielded the highest catalytic performance by maximizing enzyme loading and preventing leaching under flow conditions. The resulting microreactor demonstrated exceptional long-term stability, retaining 65% of its initial activity and maintaining <span>L</span>-DOPA production above 0.06 mM after 30 days of continuous operation. These results highlight the effectiveness of TA–Fe³⁺–mediated surface functionalization for enzyme immobilization and demonstrate the feasibility of a continuous, enzyme-based microreactor system for sustainable biochemical synthesis.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"231 ","pages":"Article 110150"},"PeriodicalIF":3.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An extended 1D dynamic model of biological methanation considering water production, variable height and maintenance heterogeneity","authors":"Julian Federico Sanchez Caldas,&nbsp;Arnaud Cockx,&nbsp;Carlos Robles Rodriguez,&nbsp;Jérôme Morchain","doi":"10.1016/j.bej.2026.110109","DOIUrl":"10.1016/j.bej.2026.110109","url":null,"abstract":"<div><div>Numerical simulation of biological methanation remains challenging due to the strong coupling between mass transfer, hydrodynamics, and bioreaction in such gas-fed bioreactors. This work presents an extension of a 1D spatio-temporal gas–liquid model for bubble columns to the case of biological methanation. To this end, three major improvements are added to the previously published model: (i) the consideration of water production due to the biological methanation reaction, (ii) the implementation of an additional equation for the liquid height, (iii) the introduction of a variable maintenance model based on the spatial heterogeneity of the H₂ mass transfer. The model was validated with experimental data from literature. Results indicate that water production acts as a dilution term, as revealed by simulations performed under both constant and variable height conditions. This led to a reduced biomass concentration while preserving methane production owing to a redistribution of H₂ consumption between maintenance and growth. These intriguing results were confirmed by analytical solutions at steady-state. The variable maintenance model further allows connecting the decrease in performances through scale-up to increased local deviations between H₂ mass transfer and cell demand. Also, a basic moving mesh method now extends the gas–liquid dynamic 1D model capabilities to any fed-batch (bio) reactor.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"230 ","pages":"Article 110109"},"PeriodicalIF":3.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146196855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A KPI-based experimental design strategy for bioprocess development","authors":"Okyanus Yazgin ,&nbsp;Martin F. Luna ,&nbsp;Peter Neubauer ,&nbsp;Ernesto C. Martinez ,&nbsp;M. Nicolas Cruz Bournazou","doi":"10.1016/j.bej.2026.110096","DOIUrl":"10.1016/j.bej.2026.110096","url":null,"abstract":"<div><div>Bioprocess development can benefit significantly from the use of mathematical models for prediction and optimization, yet the uncertainty in these models can hinder reliable early-stage decision-making for industrial-scale processes. This study introduces a telescopic model-based design of experiments approach that directly targets the reduction of uncertainty in key performance indicators (KPIs) at the optimum process conditions rather than focusing solely on model parameter precision. Using a sugarcane-to-ethanol biorefinery use case, the proposed approach is benchmarked against a traditional parameter-focused approach. Results demonstrate that the proposed strategy reduces KPI uncertainty more efficiently, identifies economically favorable process conditions faster, and prioritizes the estimation of parameters most influential on the KPI.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"230 ","pages":"Article 110096"},"PeriodicalIF":3.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146196856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogel-based colorimetric sensor using dual-enzyme active MnFe-O/C nanozymes for on-site detection of phenolic pollutants in water","authors":"YangTao Yuan ,&nbsp;JiaJia Mi ,&nbsp;QiWei Li ,&nbsp;JianPing Shi","doi":"10.1016/j.bej.2026.110098","DOIUrl":"10.1016/j.bej.2026.110098","url":null,"abstract":"<div><div>The development of portable and reliable sensors for monitoring phenolic pollutants in water remains a significant challenge. Nanozyme-based colorimetric assays offer a promising alternative to conventional methods. However, their practical application is often hindered by poor portability and stability in liquid-phase systems. To address this, we developed a novel hydrogel-based colorimetric sensor. This sensor is empowered by a dual-enzyme active nanozyme. It is designed for the on-site detection of hydroquinone (HQ) and catechol (CC). The carbon-coated MnFe oxide (MnFe-O/C) nanoparticles, derived from a MnFe-Prussian blue analogue precursor, exhibits good intrinsic oxidase- and peroxidase-like activities. Leveraging the inhibitory effect of HQ and CC on the nanozyme-catalyzed chromogenic reaction, a sensitive solution-phase colorimetric assay was established. This assay achieved detection limits of 5.78 μM for HQ and 10.43 μM for CC. Furthermore, a portable and standalone sensor was fabricated by embedding the MnFe-O/C nanozyme within a borax-crosslinked carboxymethyl cellulose hydrogel network. When coupled with a smartphone for RGB analysis, the hydrogel sensor enables on-site and quantitative detection of HQ and CC in real water samples. The recovery rates in spiked water samples ranged from 94.80 % to 99.14 % for HQ and 97.32 %-108.80 % for CC, demonstrating high accuracy and reliability. This work not only offers a viable approach for constructing dual-enzyme active nanozymes, but also provides a practical tool for detecting phenolic pollutants in water.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"229 ","pages":"Article 110098"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146075985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic mechanisms of acidification control by FeOx@HCS in stabilizing methanogenesis","authors":"Mengyue Fu,&nbsp;Yu Liu,&nbsp;Pengju Li,&nbsp;Xianliang Yi,&nbsp;Yang Liu,&nbsp;Jingjing Zhan,&nbsp;Hao Zhou","doi":"10.1016/j.bej.2026.110110","DOIUrl":"10.1016/j.bej.2026.110110","url":null,"abstract":"<div><div>Acidification frequently impairs anaerobic digestion by causing volatile fatty acid (VFA) accumulation and process collapse. Here, we report a material–microbe integrated strategy for acidification control based on mixed-valence iron oxides–encapsulated hollow carbon spheres (FeOx@HCS), which couple redox-active Fe(II/III) interfaces with a conductive carbon framework. To overcome this limitation, FeOx@HCS were synthesized via an aerosol-assisted method and introduced to a glucose-fed digester intentionally operated under acidification-prone conditions. The amendment effectively suppressed VFA accumulation (&lt;500 mg L⁻¹ vs. &gt;800 mg L⁻¹ in the control), maintained near-neutral pH, and consequently stabilized methane production. Rather than relying on external buffering, FeOx@HCS mitigated acidification by reshaping microbial community structure and improving syntrophic metabolism. Metagenomic analysis demonstrated that FeOx@HCS reshaped the microbial community by enriching methanogens such as <em>Methanoregula</em> (22.8 %) and <em>Methanothrix</em> (1.3 %), while limiting the proliferation of acidogenic bacteria. Correspondingly, the relative abundance of key methanogenic genes (POR, Ftr, Mch, Mtd, Mcr, Hdr) increased by 19–35 %. Metagenome-assembled genomes (MAG) confirmed complementary metabolic roles, with <em>Microbacter</em> facilitating acidogenesis and <em>Methanoregula/Methanosarcina</em> driving hydrogenotrophic and acetoclastic methanogenesis. Notably, genes associated with intracellular electron transfer in methanogens (Hdr, Frh) were enriched, whereas genes encoding conductive pili or cytochromes were not, suggesting a material-mediated enhancement of electron transfer rather than classical biologically driven DIET. Collectively, this study provides genome-resolved mechanistic evidence that FeOx-based carbon interfaces stabilize anaerobic digestion by coordinating acidification control, syntrophic metabolism, and electron transfer, thereby extending current Fe–C material studies beyond performance enhancement toward mechanistic interpretation.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"229 ","pages":"Article 110110"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146185300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Removal of Fe2 + from acid mine drainage by Rhodopseudomonas sphaeroides-modified lignite: Adsorption characteristics and mechanism","authors":"Wenbo An ,&nbsp;Bin Xu ,&nbsp;Junzhen Di ,&nbsp;Yifan Liu ,&nbsp;Tianzhi Wang","doi":"10.1016/j.bej.2026.110095","DOIUrl":"10.1016/j.bej.2026.110095","url":null,"abstract":"<div><div>Addressing the environmental toxicity of high-concentration heavy metal pollutants, such as Fe²⁺, in acid mine drainage (AMD) and the limited adsorption capacity of lignite, this study utilized lignite as a substrate adsorbent. By selecting the environmentally non-toxic <em>Rhodopseudomonas sphaeroides</em>, a novel low-cost modified adsorbent (<em>Rhodopseudomonas sphaeroides</em>-modified lignite, RS-L). Using batch experiments and microscopic characterization methods, the adsorption characteristics and mechanism of RS-L towards Fe²⁺ were investigated. Results indicated that natural lignite with a mesh size of 60–80 was modified with 12 mL of bacterial solution for 14 days to synthesize RS-L. When the initial Fe²⁺ concentration was 65 mg/L, pH was 4, and the dosage of RS-L was 1 g, optimal adsorption performance was achieved. The adsorption process followed the pseudo-second-order kinetic model and Langmuir monolayer adsorption, and was a spontaneous (negative Δ<em>G</em>), endothermic (positive Δ<em>H</em>), entropy-increasing (positive Δ<em>S</em>) process. At 45°C, the maximum adsorption capacity of Fe²⁺ was 13.10 mg/g. The adsorption mechanism primarily involved electrostatic adsorption, the packing effect, chemical precipitation, and complexation reactions. After five adsorption cycles, RS-L maintained an adsorption efficiency of 63.62 % and an adsorption capacity of 8.27 mg/g, demonstrating adaptability to complex ionic environments. This study offers new insights for restoring AMD and provides novel pathways for the resource utilization of lignite.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"229 ","pages":"Article 110095"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146075984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urea-based treatment promotes the propagation of antibiotic resistance genes during sludge fermentation: Insights into its multifaceted roles in structure disruption, microbial community reshaping, and metabolic regulation","authors":"Minjun Zhao ,&nbsp;Shuaijie Jin ,&nbsp;Wenzhuo Li ,&nbsp;Yuke Xu ,&nbsp;Qin Zhang","doi":"10.1016/j.bej.2026.110101","DOIUrl":"10.1016/j.bej.2026.110101","url":null,"abstract":"<div><div>Urea has been documented as an excellent promoter for improving sewage sludge (SS) fermentation, considering its effectiveness and economic feasibility, yet its effects on the fates of antibiotic resistance genes (ARGs) during this process are still unknown. Herein, the responses of ARGs distribution to urea exposure were studied, and the results revealed that urea exacerbated ARGs propagation, as evidenced by an increase of 66.8 % total abundance. Mechanistic exploration demonstrated that the presence of urea and free ammonia (FA) stripped the extracellular polymeric substances (EPS) and increased the cell membrane permeability, contributing to ARGs and mobile genetic elements (MGEs) release and consequently improved their horizontal transfer. Also, urea exhibited “screening effects” to enrich some harboring ARGs carriers (<em>e.g., Bacteroidetes_norank, Tissierella</em> and <em>Firmicutes_norank</em>). Further analysis found that the generated FA induced oxidative stress (<em>e.g., katE and SOD1</em>) and activated the SOS response (<em>e.g., recA</em>, <em>recO</em>, and <em>recR</em>), promoting ARGs formation, which could be further improved by unhydrolyzed urea through upregulating the metabolic functions (<em>e.g.,</em> TCA cycle) associated with energy production. The structural equation model suggested that the upregulation of key metabolic pathways was the predominant contributor to the ARGs propagation. Collectively, this work explored the effects and underlying mechanisms of urea on ARGs' fates during SS fermentation, highlighting the potential environmental risks of urea-based treatment on resource recovery from SS.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"229 ","pages":"Article 110101"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146075983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient biosynthesis of γ-aminobutyric acid by a GadBD304G/F433L mutant-based whole-cell biocatalyst","authors":"Fuyao Guan ,&nbsp;Min Wu ,&nbsp;Fei Tang ,&nbsp;Xin Xu ,&nbsp;Haoju Wang ,&nbsp;Qihang Chen ,&nbsp;Wei Sun ,&nbsp;Huiru Cui ,&nbsp;Jiahui Jiang ,&nbsp;Hongguang Yang ,&nbsp;Ping Yu ,&nbsp;Min Chen","doi":"10.1016/j.bej.2026.110114","DOIUrl":"10.1016/j.bej.2026.110114","url":null,"abstract":"<div><div>The inner membrane transporter GadC in <em>Escherichia coli</em> can facilitate glutamate uptake and γ-aminobutyric acid (GABA) efflux. Based on a previously engineered glutamate decarboxylase mutant GadB^D304G/F433L (with a broadened pH adaptability and an enhanced activity) expressed in <em>E. coli</em> BL21(DE3)/pETDuet-1-<em>gadB</em>^D304G/F433L, this study cloned <em>gadC</em> into the plasmid pETDuet-1-<em>gadB</em>^D304G/F433L to construct recombinant strain <em>E. coli</em> BL21(DE3)/pETDuet-1-<em>gadB</em>^D304G/F433L-<em>gadC</em>. Whole-cell biocatalysis yielded 5.1 g/L GABA with the single-gene strain and 2.3 g/L GABA with the double-gene (<em>gadB</em>^D304G/F433L-<em>gadC</em>) strain. Subsequent optimization of the biocatalytic conditions (60 °C, pH 3.2, 15 g/L cell biomass, 0.75 mM <span>L</span>-monosodium glutamate, 0.2 mM PLP) via single-factor and orthogonal experiments significantly increased GABA production to 54.84 g/L. Finally, implementing a whole-cell biocatalyst recycling strategy for 5 batches enabled the efficient synthesis of 382.59 g/L GABA from sodium glutamate. This study contributes to a possible industrial production of GABA by engineered strain <em>E. coli</em> BL21(DE3)/pETDuet-1-<em>gadB</em>^D304G/F433L.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"229 ","pages":"Article 110114"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146185298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hairpin-locked modular polymerase/nickase cycle amplification enables ultrasensitive urinary miRNA detection for acute kidney injury diagnosis","authors":"Keying Xu ,&nbsp;Xinru Liu ,&nbsp;Juan Zhu ,&nbsp;Fenghe Li ,&nbsp;Xiaoyan Liu ,&nbsp;Jie Wang","doi":"10.1016/j.bej.2026.110107","DOIUrl":"10.1016/j.bej.2026.110107","url":null,"abstract":"<div><div>The polymerase/nickase cycling amplification is a classic method for isothermal nucleic acid amplification, praised for its efficiency. However, its sensitivity and specificity are lacking for trace miRNA analysis. Herein, we have developed a novel modular polymerase/nickase cycling amplification technology (M-PNP) based on traditional polymerase/nickase probes (PNP), enhancing detection efficiency, sensitivity, and specificity. Specifically, M-PNP is formed by introducing a hairpin structure at the 5’ end of PNP and adding an auxiliary probe at the 3’ end. The hairpin structure acts like a lock, inhibiting non-specific nucleic acid amplification. In the presence of miRNA, the hairpin structure recognizes the miRNA and undergoes a conformational change, transforming into a new micro-hairpin structure. Through the action of polymerase and nickase, this transformation activates the polymerase/nickase cycling amplification reaction and promotes miRNA cycling, thereby enhancing detection sensitivity. The introduction of the auxiliary probe enables immediate activation of fluorescence signals during the target-triggered polymerase/nickase cycling amplification, significantly increasing detection speed and reducing time consumption. We used miR-21 from the urine of clinical kidney injury patients as the detection target and employed M-PNP for practical testing. The results showed that this platform can accurately distinguish kidney injury patients. This optimization and improvement injects new vitality and innovative ideas into the design of traditional polymerase/nickase cycling amplification probes.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"229 ","pages":"Article 110107"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146185326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing CHO cell productivity through a dual-pressure selection system based on tyrosine and glutamine biosynthetic pathways","authors":"Lei Cao ,&nbsp;Yunfei Shao ,&nbsp;Yang Zhou ,&nbsp;Liang Zhao ,&nbsp;Yan Zhou ,&nbsp;Qian Ye ,&nbsp;Wen-Song Tan","doi":"10.1016/j.bej.2026.110113","DOIUrl":"10.1016/j.bej.2026.110113","url":null,"abstract":"<div><div>Advances in Chinese hamster ovary (CHO) cell line development have improved monoclonal antibody (mAb) production through efficient selection systems. These systems avoid the purification complexities and biosafety concerns of antibiotic-based approaches while improving cellular metabolism. The glutamine synthetase (GS) system reduces ammonia accumulation, whereas the tyrosine biosynthesis–based triple-marker system eliminates alkaline tyrosine feeding. Despite these distinct advantages, conventional selection strategies — particularly those relying on a single marker like GS — often lack control over the light-to-heavy chain (LC/HC) ratio, which limits mAb yield. To address this, we developed a dual‑pressure selection platform through the combined disruption of the tyrosine biosynthesis pathway (triple knockout) and the <em>GS</em> gene in CHO cells. Compared to single‑pressure systems in high‑density fed‑batch and semi‑perfusion cultures, the dual‑pressure cells showed consistently higher cell-specific productivity. Under nutrient‑restricted semi‑perfusion, cell‑specific productivity increased 1.59‑fold and 5.04‑fold relative to the tyrosine and GS systems, respectively, yielding higher final titers despite lower growth. Mechanistic studies indicated that the dual‑pressure system redirects central carbon and amino acid metabolism, improving glucose utilization and lowering lactate and ammonia accumulation. It also enhances LC/HC ratio control, thereby overcoming a major limitation of GS‑only selection. This integrated strategy not only simplifies high‑density bioprocessing but also advances our understanding of metabolic selection and offers a scalable platform for gene co-expression and productivity enhancement in CHO cell engineering.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"229 ","pages":"Article 110113"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146185299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}