Biochemical Engineering Journal最新文献_第2页

Optimized solid composite bacterial agents for biodegradation of benzo[a]pyrene contaminated soils: Effects, microbial dynamic changes and mechanisms 优化固体复合菌剂对苯并[a]芘污染土壤的生物降解效果、微生物动力学变化及机制

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-16 DOI: 10.1016/j.bej.2025.109875

Bing Xiao, Jianli Jia, Xiaolong Gao, Mengyuan Zeng, Ben Zhang, Weiran Wang, Yichi Ma, Yuxin Han, Shuo Zhang

{"title":"Optimized solid composite bacterial agents for biodegradation of benzo[a]pyrene contaminated soils: Effects, microbial dynamic changes and mechanisms","authors":"Bing Xiao, Jianli Jia, Xiaolong Gao, Mengyuan Zeng, Ben Zhang, Weiran Wang, Yichi Ma, Yuxin Han, Shuo Zhang","doi":"10.1016/j.bej.2025.109875","DOIUrl":"10.1016/j.bej.2025.109875","url":null,"abstract":"<div><div>Solid composite bacterial agents (SCBA) present an innovative bioremediation strategy for the in situ degradation of benzo[<em>a</em>]pyrene (BaP), a recalcitrant compound that poses significant challenges to remediation in contaminated soils. In this study, an SCBA for BaP degradation was prepared using vacuum freeze-drying technology. Response surface methodology was employed to optimize the protective agent ratio, resulting in a composite formulation comprising 14 % skimmed milk powder, 8 % glycerol, and 8 % sucrose. This formulation achieved a freeze-drying survival rate of (89.65 %±2.04)% and an effective viable bacterial count of (1.50 ± 1.07)× 10 ¹ ¹ CFU/g in the SCBA. After 60 days of BaP-contaminated soil remediation, the BaP degradation rates in the activated bacterial liquid combined with co-metabolized substrate (CMS) (JYC) and the SCBA combined with CMS (JFC) groups were 57.23 % and 45.33 %, respectively, reflecting increases of 41.35 % and 29.45 % compared to the control (CK) group. Incorporating SCBA and CMS significantly enhanced the microbial BaP remediation efficiency and improved soil organic matter, alkaline nitrogen content, as well as fluorescein diacetate, catalase, and dehydrogenase activity. Furthermore, this approach enriched the population of beneficial bacteria involved in BaP degradation, fostering a more efficient and stable microbial network. Enhanced signaling between microorganisms facilitated faster material exchange and closer inter-bacterial contact. The CMS provided essential nutrients, enabling microorganisms to adapt more rapidly to their environment and reducing microbial remediation.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109875"},"PeriodicalIF":3.7,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering-driven precursor accumulation for enhanced biosynthesis of O-succinyl-L-homoserine via L-aspartate and L-homoserine pathway optimization 代谢工程驱动的前体积累，通过l-天冬氨酸和l-高丝氨酸途径优化促进o -琥珀酰- l-高丝氨酸的生物合成

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-15 DOI: 10.1016/j.bej.2025.109864

Jianmiao Xu , Linglong Huang , Yan Feng , Qilan Shan , Yuan Tao , Changqing Luo , Zhiqiang Liu , Yuguo Zheng

{"title":"Metabolic engineering-driven precursor accumulation for enhanced biosynthesis of O-succinyl-L-homoserine via L-aspartate and L-homoserine pathway optimization","authors":"Jianmiao Xu , Linglong Huang , Yan Feng , Qilan Shan , Yuan Tao , Changqing Luo , Zhiqiang Liu , Yuguo Zheng","doi":"10.1016/j.bej.2025.109864","DOIUrl":"10.1016/j.bej.2025.109864","url":null,"abstract":"<div><div>O-succinyl-L-homoserine (OSH) is a critical intermediate in L-homoserine synthesis and serves as a precursor for L-methionine, which is essential for various biosynthetic processes. This study employs <em>Escherichia coli</em> W3110 as the starting strain to enhance OSH production by eliminating competing and degrading pathways, while simultaneously promoting the accumulation of precursor substances. Initially, we optimized the phosphoenolpyruvate-pyruvate-oxaloacetate pathway through the overexpression of the genes <em>ppc</em>, <em>aspC</em>, <em>aspA</em>, and <em>gdhA*</em>, which significantly increased the supply of L-aspartate. In addition, we adjusted the promoters of the key enzyme genes, <em>metA</em>11 and <em>yjeH</em>, involved in the primary OSH synthesis pathway, resulting in the creation of strain OSH23. The engineered strain demonstrated notable improvements in OSH titer, yield, and productivity in a 5 L fermenter, achieving values of 131.99 g/L, 0.49 g/g, and 1.14 g/L/h, respectively. Further overexpression of <em>thrA</em><sup><em>fbr</em></sup> enhanced carbon flux, yielding strain OSH24 which produced 120.32 g/L OSH in a 5-L bioreactor with a glucose conversion of 0.51 g/g and productivity of 1.18 g/L/h. When the fermentation scale was scaled up to 50 liters, OSH24 achieved a final OSH concentration of 131.06 g/L, while maintaining a sugar acid conversion rate of 0.51 g/g. This study not only illustrates the successful application of advanced metabolic engineering for high-yield OSH production but also establishes a robust foundation for its industrial application in L-methionine biosynthesis.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109864"},"PeriodicalIF":3.7,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing the production of xylitol in recombinant Escherichia coli BL21 by metabolic engineering 利用代谢工程技术提高重组大肠杆菌BL21木糖醇的产量

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-15 DOI: 10.1016/j.bej.2025.109876

Peize Wang, Fuyao Guan, Min Wu, Chuyang Yan, Xin Xu, Haoju Wang, Qihang Chen, Wei Sun, Jianying Huang, Ping Yu

{"title":"Enhancing the production of xylitol in recombinant Escherichia coli BL21 by metabolic engineering","authors":"Peize Wang, Fuyao Guan, Min Wu, Chuyang Yan, Xin Xu, Haoju Wang, Qihang Chen, Wei Sun, Jianying Huang, Ping Yu","doi":"10.1016/j.bej.2025.109876","DOIUrl":"10.1016/j.bej.2025.109876","url":null,"abstract":"<div><div>Xylitol, a pentose sugar alcohol with sweetness comparable to sucrose, has advantageous properties such as low caloric value and anti-cariogenic characteristics, leading to its extensive applications in food, chemical, and pharmaceutical industries. Current industrial production predominantly employs chemical catalytic methods, which suffer from high cost and environmental concerns. In contrast, microbial fermentation through metabolic engineering enables direct conversion of xylose to xylitol via microbial metabolism, demonstrating superior advantages of cost-effectiveness, environmental friendliness, and mild reaction conditions. This study developed an engineered <em>Escherichia coli</em> strain for high-efficiency xylitol production by implementing three key strategies: 1) introducing the heterologous xylose reductase gene <em>xyrB</em>, 2) optimizing NADPH cofactor supply, and 3) CRISPR/Cas9-mediated knockout of the competing pathway gene <em>xylA</em>. Subsequent fermentation condition optimization achieved remarkable production performance. Under optimized conditions with 15 g/L xylose and 10 g/L glucose in a 1-L shake flask system, the engineered strain <em>E. coli</em> BL21(DE3)/<em>ΔptsGΔxylA</em>-pETDuet-1 -<em>xyrB</em>-<em>zwf</em> demonstrated maximum xylitol production of 13.8 g/L (0.92 g/g xylose), representing significant improvement in bioconversion efficiency. This study contributes to the industrial production of xylitol by engineered strain <em>E. coli</em> BL21(DE3) via metabolic engineering.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109876"},"PeriodicalIF":3.7,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144634178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KOH-impregnated oil shale biochar: Revealing mechanisms of Cu, Zn, Ni stabilization in sludge and mitigating leaching risks 氢氧化钾浸渍油页岩生物炭：揭示污泥中Cu、Zn、Ni稳定机制和降低浸出风险

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-14 DOI: 10.1016/j.bej.2025.109874

Diannan Huang , Hao Guan , Jinfan Song , Yulan Tang , Mingfu Bao , Dasong Xing , Liguo Miao , Tingting Li , Enbiao Wang

{"title":"KOH-impregnated oil shale biochar: Revealing mechanisms of Cu, Zn, Ni stabilization in sludge and mitigating leaching risks","authors":"Diannan Huang , Hao Guan , Jinfan Song , Yulan Tang , Mingfu Bao , Dasong Xing , Liguo Miao , Tingting Li , Enbiao Wang","doi":"10.1016/j.bej.2025.109874","DOIUrl":"10.1016/j.bej.2025.109874","url":null,"abstract":"<div><div>In sludge land application, secondary pollution from heavy metals remains a critical challenge. This study innovatively prepared biochar by pyrolyzing KOH-impregnated oil shale. Characterized by SEM,BET and FTIR, the obtained oil shale biochar (OS-BC) featured a porous structure with a specific surface area of 64.3226 m<sup>2</sup>/g. The effects OS-BC dosage (5 %∼40 %) and reaction time (0–20 days) on the speciation transformation of Cu, Zn, and Ni in sludge were investigated. Results showed that optimal stabilization occurred at 30 % OS-BC dosage with 10-day reaction, achieving conversion rates of 37.29 %, 35.07 %, and 46.72 % for Cu, Zn, and Ni from labile to stable fractions. Visual MINTEQ simulation and XRD analysis revealed that OS-BC increased concentrations of oxygenated ions (OH⁻, CO<sub>3</sub><sup>2-</sup>), promoting the formation of insoluble hydroxide/carbonate precipitates and stable minerals. Simulated rainfall tests showed that cumulative leaching concentrations of Cu, Zn, and Ni were reduced by 60.3 %, 80.7 %, and 79.3 % compared to the untreated group, confirming enhanced environmental safety. This study provides a novel approach to the synergistic resource utilization of oil shale and sludge.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109874"},"PeriodicalIF":3.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144634176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of heat and chloramphenicol pretreatments on sludge anaerobic bioconversion: Insights into physicochemical and microbial disruption 热和氯霉素预处理对污泥厌氧生物转化的影响：对物理化学和微生物破坏的见解

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-13 DOI: 10.1016/j.bej.2025.109872

Anam Jalil , Hikmatullah Ahmadi , Chengyu Zhang , Xiangyang Wang , Zhisheng Yu

{"title":"Impact of heat and chloramphenicol pretreatments on sludge anaerobic bioconversion: Insights into physicochemical and microbial disruption","authors":"Anam Jalil , Hikmatullah Ahmadi , Chengyu Zhang , Xiangyang Wang , Zhisheng Yu","doi":"10.1016/j.bej.2025.109872","DOIUrl":"10.1016/j.bej.2025.109872","url":null,"abstract":"<div><div>This study examines impact of heat and chloramphenicol pretreatments on sludge characteristics to improve anaerobic bioconversion under mesophilic conditions. Physicochemical, microbial, and biogas analyses were conducted to evaluate the effect of each pretreatment. Elemental composition analysis revealed that chloramphenicol pretreatment reduced the C/N ratio to 12.8 ± 0.18, whereas heat treatment maintained a more balanced ratio of 15.0 ± 0.12, compared to 13.83 in the control. Macromolecular analysis revealed extensive microbial lysis in chloramphenicol-treated sludge, reflected by significant increases in protein (43.5 ± 0.6 %) and lipid (12.0 ± 0.5 %) contents, whereas moderate changes were observed in heat-pretreated samples. The lowest VS/TS ratio (0.60 ± 0.02) in the chloramphenicol group indicated enhanced organic solubility. Biogas assays revealed that heat-pretreated sludge produced the highest cumulative yield (35.0 mL/g VS) and CH₄ content (61 %), while chloramphenicol treatment yielded slightly less gas (33.0 mL/g VS) but with an elevated H₂ content (18 %), higher than that of the control (20.4 mL/g VS). COD and TVFA reductions of 20.0 % and 77.8 %, respectively, were also notable under chloramphenicol stress. Microbial analysis revealed an enrichment of spore-forming <em>Firmicutes</em> following heat pretreatment and a hydrogenogenic shift toward <em>Chloroflexi</em> and <em>Actinobacteriota</em> under antibiotic stress. Overall, these findings demonstrated how targeted pretreatment alters sludge composition and microbial ecology to optimize biogas quality, particularly by enhancing hydrogen production for sustainable bioenergy recovery.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109872"},"PeriodicalIF":3.7,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sludge-derived biochar promoted the methane production in anaerobic digestion of thermo-alkaline pretreated waste-activated sludge: Performance and mechanisms 污泥源生物炭促进热碱性预处理污泥厌氧消化产甲烷：性能与机理

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-10 DOI: 10.1016/j.bej.2025.109861

Yin-Ping Hou , Fang-Yuan Wang , Wang-Tao Dong , Hong-Rui Ma , Bin-Bin Cai

{"title":"Sludge-derived biochar promoted the methane production in anaerobic digestion of thermo-alkaline pretreated waste-activated sludge: Performance and mechanisms","authors":"Yin-Ping Hou , Fang-Yuan Wang , Wang-Tao Dong , Hong-Rui Ma , Bin-Bin Cai","doi":"10.1016/j.bej.2025.109861","DOIUrl":"10.1016/j.bej.2025.109861","url":null,"abstract":"<div><div>The addition of biochar is a promising strategy to improve CH₄ production by strengthening direct interspecies electron transfer (DIET) in anaerobic digestion (AD). In this study, three types of sludge-derived biochar prepared at 300/500/700 °C (designated BC300, BC500, and BC700) were evaluated for their effects in improving CH₄ production efficiency and system stability during AD of thermo-alkaline pretreated waste-activated sludge (WAS). The maximum CH₄ production for the control, BC300, BC500, and BC700 groups was 326.8 mL/g VS, 401.8 mL/g VS, 423.8 mL/g VS, and 420 mL/g VS, respectively. BC500 promoted more significantly than BC300 and BC700, with 35.4 % higher maximum CH₄ production than the control group. Electrochemical analysis demonstrated that BC500 possesses both surface redox functional groups and graphitic structure, endowing it with excellent capacitance (0.31 mF/g) and electrical conductivity (3.1 ×10<sup>−4</sup> S/m), which facilitates DIET. Microbial community analysis revealed that the acetotrophic methanogens <em>Methanosaeta</em>, which are capable of participating in DIET, were enriched from 24.0 % to 32.3–34.0 % in the biochar-added groups. These findings suggest that DIET was preferentially stimulated to promote methanogenesis during WAS AD, replacing the thermodynamically unfavorable hydrogen-mediated interspecies electron transfer. This study provides a theoretical foundation for in-situ enhanced AD via sludge-derived biochar and proposes a closed-loop “treating waste with waste” strategy: improved energy recovery from WAS and sustainable sludge management in wastewater treatment plants (WWTPs).</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109861"},"PeriodicalIF":3.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144634177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a high-efficiency constitutive expression system in Salinivibrio strain for next-generation extremophile engineering 新一代嗜极微生物工程中盐弧菌高效本构表达系统的建立

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-10 DOI: 10.1016/j.bej.2025.109863

Zheng-Jun Li, Nan Pu, Bin Wei, Hao Liang

{"title":"Development of a high-efficiency constitutive expression system in Salinivibrio strain for next-generation extremophile engineering","authors":"Zheng-Jun Li, Nan Pu, Bin Wei, Hao Liang","doi":"10.1016/j.bej.2025.109863","DOIUrl":"10.1016/j.bej.2025.109863","url":null,"abstract":"<div><div>The halophilic bacterium <em>Salinivibrio</em> sp. TGB10 represents an emerging microbial chassis for industrial biotechnology, yet its genetic toolkit remains underdeveloped due to insufficient characterization of regulatory elements. Here, we identified and characterized a novel endogenous promoter (p1) through transcriptomic analysis and functional validation. The 185-bp promoter region, located upstream of a gene encoding a hypothetical protein containing leucine-zipper lipoprotein domain, was found to contain all essential regulatory elements including a functional −10 element, extended −10 motif, and −35 element. Through progressive truncation and ribosome binding site engineering, we precisely mapped these elements and revealed their compensatory interactions in transcription initiation. Next, we developed a series of synthetic promoters by randomizing the spacer region between −35 and −10 elements, achieving expression levels spanning four orders of magnitude in <em>Salinivibrio</em> and three orders in <em>Escherichia coli</em>. The practical utility of p1 promoter was demonstrated through efficient production of industrially relevant β-glucosidase, with optimized fermentation conditions yielding 76.83 U/mL activity. This study provides a critical genetic toolkit for metabolic engineering and synthetic biology applications in <em>Salinivibrio</em>.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109863"},"PeriodicalIF":3.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144614317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational designing of human senescence marker protein 30 for enhanced hydrolysis of V-type nerve agent analog 人衰老标志蛋白30的合理设计促进v型神经毒剂类似物的水解

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-10 DOI: 10.1016/j.bej.2025.109862

Priyamedha Yadav, Manik Goel, Rinkoo Devi Gupta

{"title":"Rational designing of human senescence marker protein 30 for enhanced hydrolysis of V-type nerve agent analog","authors":"Priyamedha Yadav, Manik Goel, Rinkoo Devi Gupta","doi":"10.1016/j.bej.2025.109862","DOIUrl":"10.1016/j.bej.2025.109862","url":null,"abstract":"<div><div>Organophosphate (OP) nerve agents are a class of compounds known for high toxicity and rapid action, posing a significant threat to human health. Catalytic bio-scavengers have gained substantial attention due to the limited protective efficacy and adverse side effects of chemical drugs in treating OP poisoning. However, the low catalytic efficiency remains a key limitation to their effectiveness and broader applications. Unlike existing human catalytic bio-scavengers such as huPON1, which lack activity against V-type nerve agents, the human Senescence Marker protein 30 (huSMP30) can detoxify a surrogate V-type nerve agent, i.e., Demeton-S; however, its hydrolytic activity is too low to be utilized as a drug. Therefore, in this study, the huSMP30 has been engineered through a rational protein designing approach to enhance its catalytic activity for Demeton-S. Initially, <em>in silico</em> analysis identified six key amino acid residues (E18, A62, K106, N154, D157, and D204), which were targeted for mutational analysis. Fourteen mutants, including single, double, and triple mutants, were created by site-directed mutagenesis, recombinantly produced, and analyzed <em>in vitro</em>. Substitution mutation at position 106 significantly enhanced the catalytic efficiency (<em>kcat/Km</em>) by up to ∼26-fold. In conclusion, the engineered huSMP30 may act as an effective catalytic bio-scavenger for the hydrolysis of Demeton-S; therefore, it could be utilized for the treatment of V-type nerve agent poisoning.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109862"},"PeriodicalIF":3.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144631952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of potential biomarkers for esophageal cancer based on fluorescent biosensor 基于荧光生物传感器的食管癌潜在生物标志物检测

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-10 DOI: 10.1016/j.bej.2025.109860

Liang Zhou , Tiantian Li , Shuaibing Yu , Pengnan Yao , Jinming Kong , Xueji Zhang

{"title":"Detection of potential biomarkers for esophageal cancer based on fluorescent biosensor","authors":"Liang Zhou , Tiantian Li , Shuaibing Yu , Pengnan Yao , Jinming Kong , Xueji Zhang","doi":"10.1016/j.bej.2025.109860","DOIUrl":"10.1016/j.bej.2025.109860","url":null,"abstract":"<div><div>Fluorescent biosensors have garnered significant interest due to their high sensitivity, efficiency, and rapid detection capabilities, making them an attractive alternative for various diagnostic applications. To address these needs, we developed a fluorescence-based biosensor using magnetic beads for the rapid and precise detection of miRNA-320b, a potential biomarker for esophageal cancer. The process begins by immobilizing DNA onto the surface of magnetic beads. Upon the introduction of miRNA-320b, a stable DNA-RNA duplex forms through complementary base pairing. In this biosensor design, 3-aminophenylboric acid serves as a \"crosslinker\" to connect carboxyfluorescein to miRNA-320b. The design relies on the cis-diol structure at the 3′ end of miRNA-320b, which forms a covalent bond with phenylboric acid via borate esterification, enabling the specific differentiation of miRNA-320b from DNA. The use of magnetic beads facilitates rapid separation and purification, minimizing interference and enhancing the detection of the esophageal cancer biomarker miRNA-320b. Following optimization, the biosensor displayed a linear range from 10⁻¹ ⁴ to 10⁻⁹ M with a minimum detection limit of 1.42 fM. This innovative fluorescent biosensor offers a novel, efficient, and straightforward approach for the early screening of esophageal cancer.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109860"},"PeriodicalIF":3.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144614320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimal porosity in zeolitic imidazole framework for cost-effective furfural removal in biomass hydrolysates 沸石型咪唑骨架的最佳孔隙度对生物质水解液中糠醛的经济高效去除

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-09 DOI: 10.1016/j.bej.2025.109858

Kanghong Wang , Chaozhong Xu , Wei Xiong, Shanshan Tong, Jia Ouyang, Xiaoli Gu

{"title":"Optimal porosity in zeolitic imidazole framework for cost-effective furfural removal in biomass hydrolysates","authors":"Kanghong Wang , Chaozhong Xu , Wei Xiong, Shanshan Tong, Jia Ouyang, Xiaoli Gu","doi":"10.1016/j.bej.2025.109858","DOIUrl":"10.1016/j.bej.2025.109858","url":null,"abstract":"<div><div>Efficient removal of fermentation inhibitors, particularly furan aldehydes like furfural is crucial for enhancing microbial fermentation efficiency in lignocellulosic biomass processing. In this study, zeolitic imidazolate framework-8 with different molar ratios were synthesized by regulating the Zn<sup>2 +</sup> /2-methylimidazole molar ratio (from 1:4–1:15), and their structural properties and adsorption performance were systematically evaluated. The results showed that the pore structure was crucial to the adsorption performance, and the pore structure was optimal when the Zn<sup>2+</sup>/2-methylimidazole ratio was 1:8. The optimized zeolitic imidazole framework-8 showed rapid, selective and efficient adsorption of furfural under different operating conditions, thereby preventing the formation of inhibitors from affecting fermentation while minimizing sugar loss. This study provides a generalizable design principle for the development of adsorbents in biomass conversion. This work provides a scalable solution for biomass hydrolysate detoxification, with potential cost advantages due to the material’s reusability and high adsorption capacity compared to conventional resin-based methods.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109858"},"PeriodicalIF":3.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144597066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0