Biochemical Engineering Journal最新文献_第2页

Simultaneous nitrogen and sulfur removal in a constructed rapid infiltration system with red mud/manganese-modified coke: Performance and mechanisms 赤泥/锰改性焦炭快速渗滤系统同时脱氮除硫的性能及机理

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-27 DOI: 10.1016/j.bej.2025.109842

Shuying Wang , Meixiu Lu , Shutong Dong , Shengtao Liu , Shu Gao , Yuxiang Lu , Fuyao Wei , Tingting Huang , Jieying Liu , Chengyuan Su

{"title":"Simultaneous nitrogen and sulfur removal in a constructed rapid infiltration system with red mud/manganese-modified coke: Performance and mechanisms","authors":"Shuying Wang , Meixiu Lu , Shutong Dong , Shengtao Liu , Shu Gao , Yuxiang Lu , Fuyao Wei , Tingting Huang , Jieying Liu , Chengyuan Su","doi":"10.1016/j.bej.2025.109842","DOIUrl":"10.1016/j.bej.2025.109842","url":null,"abstract":"<div><div>Water pollution from nitrogen/sulfur compounds threatens aquatic ecosystems and human health. In this study, the removal of chemical oxygen demand (COD), sulfate, and NH<sub>4</sub><sup>+</sup> -N from wastewater was compared between the constructed rapid infiltration systems (CRIS) using coke (group A), modified red mud and coke (group B), and manganese-modified coke (group C). When the concentration of sulfate was increased from 50 to 100 mg/L, the average removal rates of sulfate in groups A, B, and C were 51.8 ± 9.90 %, 53.4 ± 5.99 %, and 64.9 ± 6.50 %, and the average removal rates of NH<sub>4</sub><sup>+</sup>-N were 41.5 ± 5.69 %, 60.1 ± 5.15 %, and 50.5 ± 4.47 %. The manganese-modified coke could strengthen the treatment efficiency of the CRIS for sulfate. Microbial analyses revealed that Proteobacteria (>50 % abundance) dominated all systems, and the abundance of Brucella increased from 0.83 % to 3.53 % in group C. Furthermore, the metabolic mechanisms indicated that group C enhanced sulfur metabolism (53.3 % increase in sulfite reduction) and nitrogen cycling (elevated glycolysis/ammonia oxidation). In addition, the electron transfer efficiency in the modified red mud group was improved. These findings highlight that through manganese modification, the CRIS could coordinate sulfur and nitrogen metabolic pathways through microbial regulation, thereby providing a feasible solution for synergistic control of multiple pollutants in complex wastewater.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109842"},"PeriodicalIF":3.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feasibility of continuous-flow algal-bacterial granular sludge bioreactor with no mechanical aeration for recirculating aquaculture wastewater treatment 无机械曝气连续流藻菌颗粒污泥生物反应器处理循环水养殖废水的可行性

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-27 DOI: 10.1016/j.bej.2025.109841

Wenjun Liu , Jian Dong , Ziyin Ai, Ge Gao, Zhongfang Lei, Tian Yuan, Motoo Utsumi

{"title":"Feasibility of continuous-flow algal-bacterial granular sludge bioreactor with no mechanical aeration for recirculating aquaculture wastewater treatment","authors":"Wenjun Liu , Jian Dong , Ziyin Ai, Ge Gao, Zhongfang Lei, Tian Yuan, Motoo Utsumi","doi":"10.1016/j.bej.2025.109841","DOIUrl":"10.1016/j.bej.2025.109841","url":null,"abstract":"<div><div>Recirculating aquaculture system (RAS) can achieve high-density aquatic animal production in advanced and controlled environments. However, its application is still limited due to high resource/operation costs and difficulty in maintaining stable water quality, particularly ammonia and nitrite control. Integrating algal-bacterial aerobic granular sludge (AGS) into RAS may help reduce aeration demand and enable simultaneous C/N/P management and recovery. However, its application in continuous-flow reactors for RAS remains largely unexplored. In this study, a continuous-flow reactor with algal-bacterial AGS and no mechanical aeration was first established as the biological treatment component of RAS. The bioreactor demonstrated an excellent daily NH<sub>4</sub><sup>+</sup> -N removal capacity of 17–31 mg/g-VSS (volatile suspended solids) with > 77 % of total inorganic nitrogen removal in 4 min per cycle during the whole test period and > 90 % after 18 days’ operation in addition to 28 % N recovery. This observation was initially attributed primarily to nitrification/denitrification and subsequently transitioned to algal assimilation regarding the dominant N removal mechanism. Moreover, no external mechanical aeration was required, since high dissolved oxygen levels can be maintained by photosynthesis and water turbulence, greatly lowering energy demands. The granules also reflected strong structural stability with integrity coefficient < 20 % and good settleability. These results suggest that the proposed continuous-flow algal-bacterial AGS bioreactor is a promising solution for sustainable aquaculture industry.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109841"},"PeriodicalIF":3.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144513636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced polyhydroxybutyrate production from biomass using engineered Halomonas sp. YLGW01 with improved xylose metabolism 利用改造的Halomonas sp. YLGW01提高木糖代谢，从生物质中生产聚羟基丁酸酯

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-25 DOI: 10.1016/j.bej.2025.109840

Hyun Jin Kim , Yebin Han , Gaeun Lim , Hyun Gi Koh , See-Hyoung Park , Kyungmoon Park , Shashi Kant Bhatia , Yung-Hun Yang

{"title":"Enhanced polyhydroxybutyrate production from biomass using engineered Halomonas sp. YLGW01 with improved xylose metabolism","authors":"Hyun Jin Kim , Yebin Han , Gaeun Lim , Hyun Gi Koh , See-Hyoung Park , Kyungmoon Park , Shashi Kant Bhatia , Yung-Hun Yang","doi":"10.1016/j.bej.2025.109840","DOIUrl":"10.1016/j.bej.2025.109840","url":null,"abstract":"<div><div>Polyhydroxybutyrate (PHB) is a biodegradable polymer that can be accumulated by microorganisms as a carbon and energy storage compound. PHB is an attractive alternative to petroleum-based plastics for sustainable bioplastic production. Utilizing lignocellulosic biomass for PHB production provides environmental, economic, and sustainable benefits. Therefore, developing a strain capable of utilizing all sugars derived from lignocellulosic biomass is a practical strategy for efficient microbial production of PHB. In this study, xylose metabolism-related genes were introduced into the PHB-producing <em>Halomonas</em> sp. YLGW01 strain to enable PHB production from xylose. The introduction of xylose isomerase (<em>xylA</em>) and xylulokinase (<em>xylB</em>) enhanced xylose metabolism in <em>Halomonas</em> sp. YLGW01. Optimizing the medium composition, including xylose concentration, nitrogen source, and yeast extract concentration, resulted in a PHB titer of 0.73 g/L. The engineered strain exhibited high dry cell weight (DCW) and PHB production from xylose alone and from mixed sugars, including sucrose and glucose. Furthermore, lignocellulosic biomass hydrolysate, primarily composed of xylose and glucose, was used as the substrate, and PHB production reached 3.11 g/L with a PHB content of 61.7 %. This study is the first to effectively engineer <em>Halomonas</em> sp. YLGW01, providing an efficient strategy for microbial compound biosynthesis and demonstrating its potential for compound utilization.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109840"},"PeriodicalIF":3.7,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144513637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the application potential of transaminases: Improving thermal and pH stability of transaminase from Vibrio fluvialis through random mutagenesis 释放转氨酶的应用潜力：通过随机诱变提高河流弧菌转氨酶的热稳定性和pH稳定性

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-24 DOI: 10.1016/j.bej.2025.109839

Karen Rodríguez-Núñez , Ronny Martínez , Claudia Bernal

{"title":"Unlocking the application potential of transaminases: Improving thermal and pH stability of transaminase from Vibrio fluvialis through random mutagenesis","authors":"Karen Rodríguez-Núñez , Ronny Martínez , Claudia Bernal","doi":"10.1016/j.bej.2025.109839","DOIUrl":"10.1016/j.bej.2025.109839","url":null,"abstract":"<div><div>Transaminase from <em>Vibrio fluvialis</em> (Vfat) is a suitable enzyme for chiral amine synthesis due to its strict enantioselectivity and broad specificity for amine donors. However, like other transaminases, Vfat faces several operational challenges, particularly its stability under pH and temperature conditions— challenges could be addressed through the random mutagenesis strategy. In our research, we successfully improved two key operational properties of Vfat through random mutagenesis. A critical step in this process was developing and applying a lysis buffer, which enabled high-throughput screening in 96-well microplates. Vfat random mutagenesis library was generated through ep PCR, and the resulting library was cloned into pET28α(+)_Vfat using the MEGAWHOP method (Megaprimer PCR of Whole Plasmid). After overcoming the challenges of lysis and cloning, a library was constructed with 35 96-well microtiter plates with a mutation frequency of 2 kb<sup>−1</sup>. Variants with outstanding performance in initial activity (up to five times higher than the wild-type enzyme) and better performance at temperature challenge (six-fold increase) and in alkaline pH (retaining initial activity after incubation) were obtained. This improvement highlights the potential of random mutagenesis in this enzyme, a tool not explored in transaminases. Using a lysis buffer allowed efficient screening, thus obtaining active enzyme variants. These advances represent a significant step in using high-throughput platforms to optimize transaminases.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109839"},"PeriodicalIF":3.7,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144481054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct microbial production of 1,2-propanediol in a sugarcane biorefinery: Financial and environmental benefits from technology development 甘蔗生物精炼厂中1,2-丙二醇的直接微生物生产：技术发展带来的经济和环境效益

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-19 DOI: 10.1016/j.bej.2025.109834

Manasseh K. Sikazwe , Jeanne Louw , Johann F. Görgens

{"title":"Direct microbial production of 1,2-propanediol in a sugarcane biorefinery: Financial and environmental benefits from technology development","authors":"Manasseh K. Sikazwe , Jeanne Louw , Johann F. Görgens","doi":"10.1016/j.bej.2025.109834","DOIUrl":"10.1016/j.bej.2025.109834","url":null,"abstract":"<div><div>1,2-propanediol (1,2-PDO) is a widely used chemical with various industrial applications. Indirect biobased methods provide a sustainable alternative to petrochemical processes but are hindered by the energy-intensive and complex conversion processes. Direct biobased 1,2-PDO methods could overcome these challenges but require additional strain and bioprocess improvements. The present study quantified the technical performances required to achieve desirable financial and environmental outcomes for direct biobased 1,2-PDO compared to the fossil-based alternative. The direct biobased production of 1,2-PDO in an integrated sugarcane biorefinery was simulated using Aspen Plus® software. Two downstream processing options, reactive distillation (S1-PDO-RD) and liquid-liquid extraction (LLE) (S2-PDO-LLE), were evaluated to address process energy inefficiencies through techno-economic and environmental assessments. S2-PDO-LLE had an 8.7 % lower MSP with 2.2 % more GHG emissions than S1-PDO-RD at current bioconversion capacity, but neither scenario was economically viable. With improved bioconversion efficiencies, S2-PDO-LLE could achieve an MSP that is 19.9 % lower than the fossil-based 1,2-PDO price with 48.5 % fewer GHG emissions than the commercial method. Similarly, S1-PDO-RD could narrowly outperform the fossil-based method with 10.7 % lower GHG emissions. The factorial analysis indicated that LLE amplifies the viability gains achievable through improved strain and bioprocess performances and should be prioritized for 1,2-PDO downstream processing. Direct biobased methods have high potential to outperform incumbent petrochemical methods due to better energy efficiency and reduced reliance on yield-specific chemicals.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109834"},"PeriodicalIF":3.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolution of microplastics and changes in system performance in leachate biological treatment system 渗滤液生物处理系统中微塑料的演化及系统性能的变化

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-19 DOI: 10.1016/j.bej.2025.109832

Yiting Lian , Mingdi Yang , Xianwei Wang , Liaojia Xu , Hong Li , Chuanlong Chen , Chengran Fang

{"title":"Evolution of microplastics and changes in system performance in leachate biological treatment system","authors":"Yiting Lian , Mingdi Yang , Xianwei Wang , Liaojia Xu , Hong Li , Chuanlong Chen , Chengran Fang","doi":"10.1016/j.bej.2025.109832","DOIUrl":"10.1016/j.bej.2025.109832","url":null,"abstract":"<div><div>This study systematically investigated the evolution of four microplastics (MPs) (polystyrene (PS), polyethylene (PE), polypropylene (PP), and polyethylene terephthalate (PET)) in leachate treatment systems under aerobic and anaerobic conditions, employing surface morphology characterization (SEM) and microbial community analysis (16S rRNA sequencing) to unravel their degradation mechanisms and inhibitory effects on contaminant removal. The presence of MPs, particularly PS, PE, PP, and PET, significantly impeded the removal of chemical oxygen demand, ammonia nitrogen, and total phosphorus. Aerobic conditions resulted in initial inhibition, whereas anaerobic conditions exacerbated the degradation processes, including surface erosion and oxidation. Among the four types, PET aged more rapidly and visibly under the same conditions, while PS aged more quickly and prominently under aerobic conditions. Aerobic conditions initially suppressed microbial activity, while anaerobic conditions aggravated these effects. Changes in the microbial community, primarily composed of <em>Proteobacteria</em> and <em>Bacteroidetes</em>, reflected environmental preferences. The research emphasized the continuous need to explore MPs' evolution mechanisms within these systems to inform the development of effective pollution control strategies for practical applications. This understanding is crucial for mitigating the adverse effects of MPs on leachate treatment processes and protecting environmental health.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109832"},"PeriodicalIF":3.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Composite of Si-tag-fused heterologous enzymes and mesoporous silica for the efficient synthesis of an optically active alcohol 硅标签熔接异源酶与介孔二氧化硅的复合材料用于高效合成光活性醇

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-19 DOI: 10.1016/j.bej.2025.109836

Shun-ichi Matsuura , Takeshi Ikeda , Takako Nagase , Aritomo Yamaguchi

{"title":"Composite of Si-tag-fused heterologous enzymes and mesoporous silica for the efficient synthesis of an optically active alcohol","authors":"Shun-ichi Matsuura , Takeshi Ikeda , Takako Nagase , Aritomo Yamaguchi","doi":"10.1016/j.bej.2025.109836","DOIUrl":"10.1016/j.bej.2025.109836","url":null,"abstract":"<div><div>Efficient synthesis of high-value-added optically active alcohol ((<em>R</em>)-1-phenyl-1,2-ethanediol [(<em>R</em>)-PED]), used as pharmaceutical intermediate, was achieved by coupled enzyme reactions using mesoporous silica as the immobilization scaffold for heterologous enzymes. When heterologous enzymes, (<em>R</em>)-carbonyl reductase (RCR), and sorbitol dehydrogenase (SDH), were immobilized in ordered pores of mesoporous silica, SDH activity in coenzyme regeneration was significantly reduced. To solve this, we fused Si-tag, a silica-binding protein, to both heterologous enzymes (RCR/SDH) and attempted to orientationally immobilize them on the silica surface, leading to significant increase in (<em>R</em>)-PED yield. The yield increased approximately 5 times, from 8–10 % in heterologous enzymes without Si-tag to 40 % in Si-tag-fused heterologous enzymes. While enzyme activity remained low when Si-tag-fused heterologous enzymes were immobilized on non-porous silica support, repeated durability of Si-tag-fused heterologous enzymes immobilized on mesoporous silica improved significantly by expanding pore diameter to 10.6 nm. The (<em>R</em>)-PED yield after repeated use for 10 times was 50 % compared to the first reaction. To improve manageability of the enzyme support, a pelletized mesoporous silica powder–organic polymer composite was prepared. When Si-tag-fused heterologous enzymes immobilized on this pelletized composite were applied to (<em>R</em>)-PED synthesis, high enzyme activity and repeated durability, equivalent to mesoporous silica powder, were exhibited.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109836"},"PeriodicalIF":3.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative study of transdermal lidocaine delivery using different eutectic mixtures 不同共晶混合物经皮给药利多卡因的比较研究

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-18 DOI: 10.1016/j.bej.2025.109837

Yoshiro Tahara, Taichi Hara, Michiaki Matsumoto

{"title":"Comparative study of transdermal lidocaine delivery using different eutectic mixtures","authors":"Yoshiro Tahara, Taichi Hara, Michiaki Matsumoto","doi":"10.1016/j.bej.2025.109837","DOIUrl":"10.1016/j.bej.2025.109837","url":null,"abstract":"<div><div>Transdermal delivery can provide needle-free drug administration and avoid first-pass drug degradation in the liver, thus improving the quality of life for patients. Recently, eutectic mixtures have attracted considerable attention as components of transdermal drug delivery systems. In the present study, lidocaine was selected as a model drug, and dose-dependent transdermal delivery was evaluated using mixtures of lidocaine and menthol with different lidocaine weight fractions. The results indicated that all the eutectic mixtures exhibited enhanced skin penetration, and thus a comparative study was conducted by evaluating skin penetration in mole unit rather than mass unit. Three different lidocaine-based eutectic mixtures were prepared by adding ibuprofen, prilocaine, or menthol. Skin penetration results in the mole unit revealed that menthol was the most effective counter agent, producing eutectic mixtures with a high skin penetration effect. Mathematic analysis of the time-course cumulative amount of penetrated lidocaine in the mole unit suggested that although menthol did not enhance the diffusion of lidocaine in the skin, it enhanced the partition of lidocaine from the eutectic mixture to the outermost layer of the skin. This finding is helpful in the preparation of effective eutectic mixtures for transdermal drug delivery.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109837"},"PeriodicalIF":3.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generating 2-hydroxyl quinoline from quinoline through a single mono-oxygenation by Delftia lacustris 湖三角洲藻单氧氧化喹啉生成2-羟基喹啉

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-18 DOI: 10.1016/j.bej.2025.109835

Haiyun Zhang , Qiguo Zhong , Fei Liu , Linlin Chen , Yongming Zhang , Bruce E. Rittmann

{"title":"Generating 2-hydroxyl quinoline from quinoline through a single mono-oxygenation by Delftia lacustris","authors":"Haiyun Zhang , Qiguo Zhong , Fei Liu , Linlin Chen , Yongming Zhang , Bruce E. Rittmann","doi":"10.1016/j.bej.2025.109835","DOIUrl":"10.1016/j.bej.2025.109835","url":null,"abstract":"<div><div>Quinoline and its derivatives are nitrogenous heterocyclic compounds with biological and pharmaceutical activities. 2-hydroxyl quinoline (2-HQ), a representative derivative, currently is synthesized through chemical methods that have high energy consumption and secondary-pollution risks. In this work, 2-HQ was generated from quinoline by a strain of <em>Delftia lacustris</em> that was isolated from quinoline-acclimated sludge. <em>D</em>. <em>lacustris</em> carried out only one step mono-oxygenation of quinoline, which transformed it into 2-HQ. 2-HQ was not further transformed unless quinoline disappeared completely. When succinate was added as an exogenous electron donor, quinoline transformation was significantly accelerated: the rate was increased by 89 % and 120 % with 0.1-mM and 0.2-mM succinate added, respectively, which supports that 2-HQ was product of quinoline mono-oxygenation requiring an intracellular electron donor. The produced 2-HQ corresponded to a standard sample of 2-HQ according to Fourier Transform Infrared and Nuclear Magnetic Resonance analyses, and the purity of the 2-HQ reached ∼80 % when inorganic salts were removed from the medium. Genomic and transcriptomic analyses confirmed the presence and expression of FAD-dependent mono-oxygenase and FMNH2-dependent alkanesulfonate monooxygenase in <em>D</em>. <em>lacustris</em>. As the economic value of 2-HQ is more than five-thousand-fold higher than quinoline, these results open the door for a promising new path for green biosynthesis that also is cleaner way to produce 2-HQ.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109835"},"PeriodicalIF":3.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of ureolysis and microbially induced calcium carbonate precipitation under different incubation and reaction conditions 不同孵育和反应条件下尿素溶解和微生物诱导碳酸钙沉淀的表征

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-16 DOI: 10.1016/j.bej.2025.109827

Hyun-Woo Joo , Matthew H. Fyfe , Irene Verdú , Seyed Ali Rahmaninezhad , Christopher M. Sales , Wil V. Srubar III , Mija H. Hubler

{"title":"Characterization of ureolysis and microbially induced calcium carbonate precipitation under different incubation and reaction conditions","authors":"Hyun-Woo Joo , Matthew H. Fyfe , Irene Verdú , Seyed Ali Rahmaninezhad , Christopher M. Sales , Wil V. Srubar III , Mija H. Hubler","doi":"10.1016/j.bej.2025.109827","DOIUrl":"10.1016/j.bej.2025.109827","url":null,"abstract":"<div><div>Understanding the factors influencing ureolysis and calcium carbonate precipitation is essential for optimizing microbially induced calcium carbonate precipitation (MICP) processes, yet comparative insights into different bacterial species under varying conditions remain inconclusive. This study investigates the ureolysis and MICP characteristics of <em>Sporosarcina pasteurii</em> and <em>Lysinibacillus sphaericus</em> under varying incubation and reaction conditions. Batch experiments were conducted to assess the effects of incubation duration and pH, bacterial cell density, and reagent (<em>i.e.</em>, urea and calcium ion) concentrations on ureolysis and CaCO<sub>3</sub> precipitation. The findings indicate that both bacteria exhibit higher urease activity after 24 h of incubation compared to 48 h, regardless of incubation pH. <em>S. pasteurii</em> exhibits maximum specific urease activity when incubated at an alkaline pH (pH 9.0), whereas <em>L. sphaericus</em> peaks after incubation at a neutral pH (pH 7.2). Moreover, the ureolysis performance of <em>S. pasteurii</em> shows greater sensitivity to incubation conditions, while <em>L. sphaericus</em> maintains consistently. At low cell density (<em>OD</em><sub>600</sub> ∼0.1), specific urease activity and early-stage mineralization efficiency are improved, whereas high density (<em>OD</em><sub>600</sub> ∼1.0) enhances overall CaCO<sub>3</sub> precipitation and prolonged vaterite presence. High reagent concentrations (1.0 and 1.5 M) further enhance urease activity and precipitation rates and lead to vaterite dominance (> 93 %), while low concentration (0.3 M) favors calcite formation over time. These findings provide insights into evaluating and optimizing MICP performance under varying incubation and reaction conditions.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109827"},"PeriodicalIF":3.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144297985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0