Detection of potential biomarkers for esophageal cancer based on fluorescent biosensor

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2025-07-10 DOI:10.1016/j.bej.2025.109860

Liang Zhou , Tiantian Li , Shuaibing Yu , Pengnan Yao , Jinming Kong , Xueji Zhang

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引用次数: 0

Abstract

Fluorescent biosensors have garnered significant interest due to their high sensitivity, efficiency, and rapid detection capabilities, making them an attractive alternative for various diagnostic applications. To address these needs, we developed a fluorescence-based biosensor using magnetic beads for the rapid and precise detection of miRNA-320b, a potential biomarker for esophageal cancer. The process begins by immobilizing DNA onto the surface of magnetic beads. Upon the introduction of miRNA-320b, a stable DNA-RNA duplex forms through complementary base pairing. In this biosensor design, 3-aminophenylboric acid serves as a "crosslinker" to connect carboxyfluorescein to miRNA-320b. The design relies on the cis-diol structure at the 3′ end of miRNA-320b, which forms a covalent bond with phenylboric acid via borate esterification, enabling the specific differentiation of miRNA-320b from DNA. The use of magnetic beads facilitates rapid separation and purification, minimizing interference and enhancing the detection of the esophageal cancer biomarker miRNA-320b. Following optimization, the biosensor displayed a linear range from 10⁻¹ ⁴ to 10⁻⁹ M with a minimum detection limit of 1.42 fM. This innovative fluorescent biosensor offers a novel, efficient, and straightforward approach for the early screening of esophageal cancer.

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基于荧光生物传感器的食管癌潜在生物标志物检测

荧光生物传感器由于其高灵敏度、高效率和快速检测能力而获得了极大的兴趣，使其成为各种诊断应用的有吸引力的替代方案。为了满足这些需求，我们开发了一种基于荧光的生物传感器，使用磁珠快速准确地检测miRNA-320b，这是一种潜在的食管癌生物标志物。这个过程首先将DNA固定在磁珠表面。引入miRNA-320b后，通过互补碱基配对形成稳定的DNA-RNA双链。在这种生物传感器设计中，3-氨基苯基硼酸作为“交联剂”连接羧基荧光素和miRNA-320b。该设计依赖于miRNA-320b 3 '端的顺式二醇结构，通过硼酸酯化与苯硼酸形成共价键，使miRNA-320b与DNA特异性分化。磁珠的使用有助于快速分离和纯化，最大限度地减少干扰，增强对食管癌生物标志物miRNA-320b的检测。经过优化，生物传感器显示出从10⁻¹ ⁴到10⁻⁹M的线性范围，最小检测限为1.42 fM。这种创新的荧光生物传感器为食管癌的早期筛查提供了一种新颖、高效、直接的方法。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.