Biochemical Engineering Journal最新文献_第3页

Energy-guided accumulated mutation strategy achieves a highly efficient polyethylene terephthalate-degrading enzyme

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-08 DOI: 10.1016/j.bej.2025.109708

Zhi Wang , Jiaxing Zhang , Shengping You , Rongxin Su , Wei Qi

{"title":"Energy-guided accumulated mutation strategy achieves a highly efficient polyethylene terephthalate-degrading enzyme","authors":"Zhi Wang , Jiaxing Zhang , Shengping You , Rongxin Su , Wei Qi","doi":"10.1016/j.bej.2025.109708","DOIUrl":"10.1016/j.bej.2025.109708","url":null,"abstract":"<div><div>The extensive application of polyethylene terephthalate (PET) has led to severe environmental pollution. Enzymatic degradation provides a sustainable solution for PET recycling, but the limited hydrolytic activity of current PET hydrolases hinders its practical application. Here, we designed an Energy-Guided Accumulated Mutation Strategy (EGAMS) for the rational design of PET enzyme PHL7. By applying multiple rounds of evolution to enhance the degradation activity and thermostability of PHL7, we achieved the excellent PET degradation enzyme FlashPETase (PHL7<sup>E148K/T158P/S184E/H185Y</sup>) with 238.8 % PET degradation activity of PHL7 and 82.93 °C melting temperature. FlashPETase completely degrades PET at 72 °C with an enzyme loading of 1.5 mg<sub>enzyme</sub> g<sub>PET</sub><sup>−1</sup>, without any substrate pretreatment or additional processing. As for PET substrates with high crystallinity, FlashPETase still performs well. Furthermore, molecular simulations revealed the structural mechanisms of FlashPETase’s enhanced activity and stability. In summary, the EGAMS protocol and the variants discussed in this work advance the fields of PET degradation and enzyme engineering, offering valuable insights for future research.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"219 ","pages":"Article 109708"},"PeriodicalIF":3.7,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of temperature switching on nitrogen removal and effluent S/N ratio via sulfur autotrophic denitrification

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-06 DOI: 10.1016/j.bej.2025.109703

Xiaoyan Dang , Xu Li , Chaoyue Zhao , Yanping Zhang , Xiaolong Gao , Shumin He , Jie Han , Yaonan Zhu , Youzhao Wang , Tong Zhu

{"title":"Impact of temperature switching on nitrogen removal and effluent S/N ratio via sulfur autotrophic denitrification","authors":"Xiaoyan Dang , Xu Li , Chaoyue Zhao , Yanping Zhang , Xiaolong Gao , Shumin He , Jie Han , Yaonan Zhu , Youzhao Wang , Tong Zhu","doi":"10.1016/j.bej.2025.109703","DOIUrl":"10.1016/j.bej.2025.109703","url":null,"abstract":"<div><div>Seasonal temperature alternation severely affects the biological denitrification of high nitrogen concentrations in industrial wastewater. This study used sulfur-based filled particles for the sulfur autotrophic denitrification technique, and evaluated the performance of the sulfur autotrophic denitrification (SAD) reactor for the removal of high concentrations of NO<sub>3</sub><sup>-</sup>-N by simulating seasonal shifts and switching between 10℃ and 30℃ for 275 days. By precisely adjusting influent nitrate-nitrogen and Hydraulic Retention Times <strong>(</strong>HRTs<strong>)</strong>, studies revealed enhanced nitrogen removal loading (NRL) at 30℃. Growth of sulfur autotrophs and electron transfer were inhibited at 10℃, and metabolites and biological residues supplied organic matter to heterotrophic denitrifying bacteria, resulting in lower sulfate production and significantly lower S/N ratios than at 30℃. Response surface optimization indicated optimal conditions: 83 % denitrification rate at 27.94℃, 18.7 h HRT, and 135.79 mg/L influent concentration, aligning closely with experimental findings. <em>Thiobacillus</em> and <em>Thermomonas</em> were more abundant at 30℃. And higher correlation of microorganisms between the two samples at high and low temperatures under high-concentration influent conditions. Additionally, the application of the SAD system is economically feasible because its raw materials are low-cost sulfur-based materials. This study aims to demonstrate the advantages of temperature in sulfur autotrophic denitrification, enhance the understanding of its mechanism, and provide a basis for wastewater treatment applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"219 ","pages":"Article 109703"},"PeriodicalIF":3.7,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143593352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective expression of taste-modifying protein miraculin fusing FMDV 2A gene in Chlamydomonas reinhardtii

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-06 DOI: 10.1016/j.bej.2025.109706

Chuan Wang, Xiaomei Wang, Ge Rang Lamu, Wei Liang, Yaomei Tian

{"title":"Effective expression of taste-modifying protein miraculin fusing FMDV 2A gene in Chlamydomonas reinhardtii","authors":"Chuan Wang, Xiaomei Wang, Ge Rang Lamu, Wei Liang, Yaomei Tian","doi":"10.1016/j.bej.2025.109706","DOIUrl":"10.1016/j.bej.2025.109706","url":null,"abstract":"<div><div>Miraculin is a taste modification protein derived from plants, which can change sour taste into sweet taste, and has great application potential in food industry. Currently, the extraction of miraculin presented some problems such as cumbersome process and low purity. <em>Chlamydomonas reinhardtii</em> is a potential expression system of exogenous proteins. However, the exogenous genes are integrated randomly in genome of <em>C. reinhardtii</em> during transformation, which leads to significant expression variability among transformants. The 2A peptide from FMDV virus has been proven to effectively improve the expression efficiency of exogenous proteins in multi-gene expression systems through self-cleavage mechanism. In presented study, the expression vector pSP108–2A-miraculin containing 2A-miraculin fusion gene was constructed and transformed into <em>C. reinhardtii</em> by glass bead method. Two positive strains were obtained through bleomycin resistance selecting. The detection results showed that miraculin gene was integrated into the genome of <em>C. reinhardtii</em> cells at the molecular level, and was successfully expressed at the protein level. This study was the first time to express the miraculin in <em>C. reinhardtii</em>, which provided an optional way to obtain miraculin.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"219 ","pages":"Article 109706"},"PeriodicalIF":3.7,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143593353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization modeling and economics assessment on simultaneous struvite and bioelectricity production from waste nutrient solution in the microbial fuel cell

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-04 DOI: 10.1016/j.bej.2025.109705

Sreelakshmi C , Kiruthika S , Jeyalakshmi R

{"title":"Optimization modeling and economics assessment on simultaneous struvite and bioelectricity production from waste nutrient solution in the microbial fuel cell","authors":"Sreelakshmi C , Kiruthika S , Jeyalakshmi R","doi":"10.1016/j.bej.2025.109705","DOIUrl":"10.1016/j.bej.2025.109705","url":null,"abstract":"<div><div>Several Sustainable Development Goals (SDGs) emphasize community well-being and environmental health. Addressing food and energy demands while ensuring a clean environment remains a global challenge. This study investigates nutrient recovery as struvite and bioelectricity generation from nutrient-rich wastewater using a dual-chamber microbial fuel cell (MFC). Response Surface Methodology with Central Composite Design (CCD) optimized Phosphorus (P) recovery at minimal cost. Simulation parameters, including the N:P ratio, chemical oxygen demand (COD), and cathodic aeration rate, were tested. Results showed that P recovery efficiency was influenced by the N:P ratio and COD, while power density was primarily impacted by COD. Optimized conditions of N:P = 1, COD = 3000 mg/L, aeration = 150 mL/min achieved 90 % P recovery as magnesium ammonium phosphate hexahydrate (struvite, MgNH₄PO₄·6H₂O) with a power density of 860 mW/m². The estimated cost based on CCD simulations was 2.5–2.6 USD/m³ within a 5 % deviation under optimal conditions of N:P = 2, COD = 3000 mg/L, aeration = 150 mL/min. These findings highlight the potential of integrating MFCs into wastewater treatment plants (WTPs) for nutrient recovery, COD reduction, and bioelectricity production. This approach offers a viable solution for sustainable wastewater management but requires careful assessment of Technology Readiness Level (TRL) and System Readiness Level (SRL) for large-scale implementation.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"218 ","pages":"Article 109705"},"PeriodicalIF":3.7,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical production from sustainable carbon sources by Komagataella phaffii

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-03 DOI: 10.1016/j.bej.2025.109702

Wajeeha A. Raja , Pınar Çalık

{"title":"Biochemical production from sustainable carbon sources by Komagataella phaffii","authors":"Wajeeha A. Raja , Pınar Çalık","doi":"10.1016/j.bej.2025.109702","DOIUrl":"10.1016/j.bej.2025.109702","url":null,"abstract":"<div><div>The use of sustainable carbon sources (SCSs) for biochemical production is needed to facilitate the chemical process industries transition towards the net-zero age and greener economy. The use of SCSs such as crude glycerol, ethanol, methanol, acetate, lactate, and formate offers potential cost reduction and sustainability benefits. However, the integration of SCSs into bioindustries is generally challenged by their low uptake rates, toxicity, and the metabolic stress they exert. Therefore, their transport rates from fermentation medium into the cell and then sequential intracellular reaction rates towards target products need to be fine-tuned. This review begins with the importance of use of SCSs in biochemical process industries and continues with the current state of metabolic engineering strategies employed to optimize biochemical and recombinant protein production. In turn, metabolic engineering strategies to increase the uptake rates of two SCSs, ethanol and acetate, and the carbon fluxes fueling the TCA cycle in <em>Komagataella phaffii</em> (<em>Pichia pastoris</em>) are discussed. Moreover, the latest findings on bioreactor operation condition optimization are compiled with a special emphasis on recombinant protein production. We conclude that the future of <em>K. phaffii</em>-based bioprocessing lies in integrating metabolic and bioreactor engineering with systems biology, while advances in synthetic biology, such as modular genome editing and machine learning for promoter and pathway design, will further refine the metabolic versatility of <em>K. phaffii</em>.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"219 ","pages":"Article 109702"},"PeriodicalIF":3.7,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational design based on translation pausing theory significantly enhances the soluble expression and activity of multidomain anti-CD20 fab antibody sequences

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-03 DOI: 10.1016/j.bej.2025.109704

Aiping Kong , Shiwen Chen , Wenxi Huang , Xinshan Xie , Qiuling Xie , Sheng Xiong

{"title":"Rational design based on translation pausing theory significantly enhances the soluble expression and activity of multidomain anti-CD20 fab antibody sequences","authors":"Aiping Kong , Shiwen Chen , Wenxi Huang , Xinshan Xie , Qiuling Xie , Sheng Xiong","doi":"10.1016/j.bej.2025.109704","DOIUrl":"10.1016/j.bej.2025.109704","url":null,"abstract":"<div><div>The expression of IgG antibodies in Chinese hamster ovary (CHO) cells has been widely used, but their expression in <em>Escherichia coli</em> remains challenging. In antibody engineering and drug research, the expression and functional study of antibody fragments in <em>E. coli</em> is crucial for early development. However, these fragments often aggregate to form inclusion bodies or exhibit low solubility when expressed in the periplasm, making them difficult to obtain. In this study, we propose a strategy based on the theory of translational pausing, where mutations are introduced into the DNA sequence to alter the translation pausing sites without changing the amino acid sequence, thus promoting correct protein folding and improving the solubility of heterologous proteins in <em>E. coli</em>. We previously successfully optimized the antiviral protein cyanovirin-N (CVN) from cyanobacteria and epoxide hydrolase (EH-Ar) from Agrobacterium, and we attempted to express an anti-CD20 Fab antibody with a quaternary structure to verify whether the translational pausing technique can increase the solubility and expression of multidomain proteins. Compared with the wild-type sequence, the designed coding sequence exhibited greater soluble expression in <em>E. coli</em>, and the expressed Fab antibody retained its activity. This study further demonstrates the importance of translational pausing in the expression of heterologous proteins in bacterial hosts and provides a novel approach for soluble expression and rapid preparation of recombinant small-molecule antibodies.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"218 ","pages":"Article 109704"},"PeriodicalIF":3.7,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Scaling iPSC production in different 3D platforms in suspension culture for their use in allogeneic regenerative therapies” [Biochem. Eng. J. 217 (2025) 109672]

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-01 DOI: 10.1016/j.bej.2025.109701

Danton Freire-Flores , Nyna Kawles , Pablo Caviedes , Barbara A. Andrews , Juan A. Asenjo

引用次数: 0

Maximizing harvesting of Chlorella vulgaris via calcium oxide nanoparticle-modified fungal pellet using response surface methodology

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-01 DOI: 10.1016/j.bej.2025.109700

Muhammad Hizbullahi Usman , Mohd Farizal Kamaroddin , Mohd Helmi Sani , Aliyu Ibrahim Dabai , Abdulrahman Sani Aliero , Ali El-Rayyes

{"title":"Maximizing harvesting of Chlorella vulgaris via calcium oxide nanoparticle-modified fungal pellet using response surface methodology","authors":"Muhammad Hizbullahi Usman , Mohd Farizal Kamaroddin , Mohd Helmi Sani , Aliyu Ibrahim Dabai , Abdulrahman Sani Aliero , Ali El-Rayyes","doi":"10.1016/j.bej.2025.109700","DOIUrl":"10.1016/j.bej.2025.109700","url":null,"abstract":"<div><div>This study aims to optimize the harvesting of <em>Chlorella vulgaris</em> using calcium oxide nanoparticle modified-<em>Aspergillus pseudonomiae</em> pellets (AP@CaONP). To maximize harvesting efficiency, response surface methodology (RSM-CCD) was applied to statistically optimize key parameters, including mycelial dosage (%), agitation speed (rpm), and pH. Lipid yield from harvested <em>C. vulgaris</em> biomass under optimized conditions was analyzed, and resulting fatty acid methyl esters were identified using Gas Chromatography-Mass Spectrometry (GC-MS). Additionally, the recycling potential of spent medium for recultivation of <em>C. vulgaris</em> was evaluated. Scanning Field Emission Scanning Electron Microscopy (FESEM), Fourier Transform Infrared spectroscopy (FTIR), and zeta potential analyses were conducted to confirm the interaction between AP@CaONP and <em>C. vulgaris</em>. The results show that under optimized conditions (6 % mycelial dosage, 170 rpm, pH 5.5), harvesting efficiency reached 99.24 % in 60 minutes with only a 0.2 % deviation between predicted and experimental values. Lipid yield from harvested biomass reached 26.98 %, and GC-MS revealed fatty acids including C16:0, C16:1, C16:2, C18:0, C18:1, and C18:2. <em>C. vulgaris</em> showed similar growth patterns in fresh and recycled BG-11 media, indicating minimal impact on biomass productivity and reduced water consumption. This optimized AP@CaONP-based harvesting method offers a practical, eco-friendly approach for efficient microalgae biomass recovery, supporting sustainable biodiesel production from <em>Chlorella</em> species.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"219 ","pages":"Article 109700"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous enzymatic hydrolysis and bioconversion of deacetylated and disk refined sugarcane bagasse to single-cell protein: An experimental investigation and techno-economic analysis

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-24 DOI: 10.1016/j.bej.2025.109691

Veronica Lourens, Catharine E. Bosman , Abdul M. Petersen , Gerhardt Coetzee, Johann F. Görgens , Eugéne van Rensburg

{"title":"Simultaneous enzymatic hydrolysis and bioconversion of deacetylated and disk refined sugarcane bagasse to single-cell protein: An experimental investigation and techno-economic analysis","authors":"Veronica Lourens, Catharine E. Bosman , Abdul M. Petersen , Gerhardt Coetzee, Johann F. Görgens , Eugéne van Rensburg","doi":"10.1016/j.bej.2025.109691","DOIUrl":"10.1016/j.bej.2025.109691","url":null,"abstract":"<div><div>Rising animal feed costs are driving up animal agriculture and food prices. While single-cell protein (SCP) production from lignocellulosic waste offers a solution, pretreatment of these feedstocks is required. In this study, the SCP yield from sugarcane bagasse pretreated via deacetylation in combination with disk refining (DDR), prior to simultaneous enzymatic hydrolysis and fermentation using <em>P. ostreatus</em> and <em>F. venenatum</em> compared to <em>S. cerevisiae</em>, was investigated. A biorefinery was simulated to investigate the economic feasibility of animal feed enhanced with SCP, using a discounted cashflow analysis. Deacetylation and DDR were effective pretreatment approaches, improving enzymatic digestibility from 9.10 g sugar/100 g dry matter (DM) to 75.75 and 80.14 g sugar/100 g DM, respectively, which was comparable to steam-explosion pretreatment. Pretreated bagasse was successfully converted to SCP through simultaneous enzymatic hydrolysis and bioconversion. However, DDR was preferred for bioconversions using <em>P. ostreatus</em> and <em>F. venenatum</em>, achieving SCP yields of 4.24 ± 0.17 and 3.47 ± 0.33 g protein/100 g DM, respectively. In the ranges investigated, solids loading had a more pronounced effect on the SCP yield than enzyme dosage, irrespective of the microorganism used. However, the minimum selling price of animal feed enhanced with SCP (0.69 US$/kg) remains higher than the market price of animal feeds like soybean meal (0.40 US$/kg). The technology shows promise but can be further optimised.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"218 ","pages":"Article 109691"},"PeriodicalIF":3.7,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergy between horseradish peroxidase HRP and edible gelatin: A simple way to remove phenols and dyes simultaneously from wastewater

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-23 DOI: 10.1016/j.bej.2025.109692

Jianling Feng , Huadeng Li , Ke Zheng , Yanbin Zeng , Guanqun Xie , Xiaoxia Wang

{"title":"Synergy between horseradish peroxidase HRP and edible gelatin: A simple way to remove phenols and dyes simultaneously from wastewater","authors":"Jianling Feng , Huadeng Li , Ke Zheng , Yanbin Zeng , Guanqun Xie , Xiaoxia Wang","doi":"10.1016/j.bej.2025.109692","DOIUrl":"10.1016/j.bej.2025.109692","url":null,"abstract":"<div><div>Wastewater containing phenols or dyes is quite common. Given the potential harmful effects of such wastewater on both the environment and human health, there is a continuing demand for efficient, green, and cost-effective methods to remove these pollutants. In this study, edible gelatin was employed as an additive in conjunction with horseradish peroxidase (HRP) to purify wastewater containing phenols and dyes. Under optimized conditions, the combination of HRP and gelatin effectively purified various types of wastewater containing different phenols, achieving removal rates of up to 90 %. Furthermore, this strategy was successfully applied to the degradation of mixed wastewater containing dyes (methyl orange, methylene blue or/and rhodamine B) in the presence of phenol. High removal rates for both phenol and the dyes were also observed and the TOC removal rate reached as high as 94 %. Finally, a removal mechanism involving the HRP/gelatin system was proposed based on UV-Vis detection, particle size analysis, LC-MS, and TOC results. This green and efficient strategy for the removal of phenols and dyes from wastewater represents a valuable contribution to sustainable environmental remediation.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"218 ","pages":"Article 109692"},"PeriodicalIF":3.7,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143508784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0