Biochemical Engineering Journal最新文献_第3页

Advances in bioactivities, cultivation strategies, genetic engineering, and multifaceted applications of Euglena gracilis 细叶菊的生物活性、栽培策略、基因工程及多方面应用研究进展

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-05-01 Epub Date: 2026-01-21 DOI: 10.1016/j.bej.2026.110087

Zhuoyao Li , Hongyu Zhang , Wenjun Song , Tingting Lou , Suying Wang , Sisi Niu , Jun Liu , Jin Hua

{"title":"Advances in bioactivities, cultivation strategies, genetic engineering, and multifaceted applications of Euglena gracilis","authors":"Zhuoyao Li , Hongyu Zhang , Wenjun Song , Tingting Lou , Suying Wang , Sisi Niu , Jun Liu , Jin Hua","doi":"10.1016/j.bej.2026.110087","DOIUrl":"10.1016/j.bej.2026.110087","url":null,"abstract":"<div><div><em>Euglena gracilis</em> (<em>E. gracilis</em>) is a unique single-celled eukaryotic microalga that lacks a rigid cell wall, which endows it with remarkable morphological plasticity and environmental resilience. It has attracted extensive attention from both academia and industry owing to its rich repertoire of bioactive metabolites, exceptional adaptability to fluctuating environments, and broad biotechnological potential. <em>E. gracilis</em> synthesizes a wide range of bioactive components, including β-carotene, paramylon, vitamins, and fatty acids. Moreover, it uniquely combines the photosynthetic autotrophy typical of plant cells with the heterotrophic metabolism characteristic of animal cells, providing a model for cross-kingdom metabolic integration and enhancing its ecological competitiveness. However, despite its capability for autotrophic, heterotrophic, and mixotrophic cultivation, its large-scale commercial exploitation remains challenged by environmental sensitivity and limited market recognition. This review systematically outlines the morphology, bioactivities, and cultivation strategies of <em>E. gracilis</em>, critically assessing how growth conditions influence biomass and metabolite profiles. Furthermore, advances in genetic engineering aimed at enhancing strain robustness, productivity, and functional compound biosynthesis are reviewed, along with their emerging applications in food, health, bioenergy, and environmental fields. Collectively, this work highlights the value of <em>E. gracilis</em> as a multifunctional and sustainable biological platform, providing a scientific and practical foundation for its future industrial applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"229 ","pages":"Article 110087"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced physicochemical properties and tyrosinase inhibition of curcumin via glycosylation by Bacillus velezensis LQ5 in a non-aqueous system velezensis LQ5在非水体系中通过糖基化增强姜黄素的理化性质和酪氨酸酶抑制作用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-05-01 Epub Date: 2026-02-05 DOI: 10.1016/j.bej.2026.110111

Jinhui Li , Zhihao He , Sen Zhang , Wenxin Ji, Yanqing Zhang, Junyun Guo, Jinao Duan, Lisi Zou, Liangliang Chen

{"title":"Enhanced physicochemical properties and tyrosinase inhibition of curcumin via glycosylation by Bacillus velezensis LQ5 in a non-aqueous system","authors":"Jinhui Li , Zhihao He , Sen Zhang , Wenxin Ji, Yanqing Zhang, Junyun Guo, Jinao Duan, Lisi Zou, Liangliang Chen","doi":"10.1016/j.bej.2026.110111","DOIUrl":"10.1016/j.bej.2026.110111","url":null,"abstract":"<div><div>The unique chemical structure of curcumin is a double-edged sword, responsible for its diverse bioactivities but also for its poor aqueous solubility and instability. To resolve this dilemma, a targeted structural modification strategy, microbial glycosylation, was employed. In this study, curcumin 4’-O-β-<span>D</span>-glucoside (CG) was biosynthesized in a non-aqueous system using <em>Bacillus velezensis</em> LQ5, introducing a glucose moiety without disrupting the essential pharmacophore of curcumin. Following the optimization of fermentation conditions, a molar yield of 55.48 % for CG was achieved within 9 h. This single glycosylation conferred concurrent and remarkable improvements in key properties: a dramatic 2153-fold enhancement in water solubility, superior photothermal stability (e.g., ∼65 % retention in solution at 100 °C for 6 h vs. < 40 % for curcumin), and a concurrently enhanced tyrosinase inhibitory activity (IC<sub>50</sub> = 119.15 μmol/L), representing an approximately ∼28 % increase in potency over curcumin, which confirms its place among active glycosides. This work validates microbial glycosylation as an effective strategy for optimizing property-limiting structures, offering a paradigm for the development of other insoluble natural products.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"229 ","pages":"Article 110111"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146185327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of free and CLEA-immobilised α-amylase and α-glucosidase from Anoxybacillus flavithermus 2641 T 黄热无氧杆菌2641 游离α-淀粉酶和α-葡萄糖苷酶与固定化α-淀粉酶的比较分析

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-05-01 Epub Date: 2026-01-30 DOI: 10.1016/j.bej.2026.110106

Emel Alemdaroğlu , Fulya Ay , Dilşat Nigar Çolak , Halil İbrahim Güler , Ali Osman Belduz

{"title":"Comparative analysis of free and CLEA-immobilised α-amylase and α-glucosidase from Anoxybacillus flavithermus 2641 T","authors":"Emel Alemdaroğlu , Fulya Ay , Dilşat Nigar Çolak , Halil İbrahim Güler , Ali Osman Belduz","doi":"10.1016/j.bej.2026.110106","DOIUrl":"10.1016/j.bej.2026.110106","url":null,"abstract":"<div><div>α-Amylase (EC 3.2.1.1) and α-glucosidase (EC 3.2.1.20) are key enzymes in starch hydrolysis, widely applied in biotechnological and food industries. In this study, recombinant α-amylase (AflAmy) and α-glucosidase (AflGlu) from <em>Anoxybacillus flavithermus</em> 2641 <sup>T</sup> were purified using a cobalt affinity column, yielding proteins of approximately 50 kDa as confirmed by SDS-PAGE. Both enzymes were immobilised through the cross-linked enzyme aggregate (CLEA) method. Optimal CLEA preparation involved 96 % ammonium sulfate saturation at 4 °C for 30 min, followed by cross-linking with 5 mM glutaraldehyde for 2–3 h at room temperature. Free AflAmy exhibited optimal activity at pH 8.0 and 70 °C, while immobilization shifted its optimum to pH 9.0. Free AflGlu was most active at pH 8.0 and 60 °C, changing to 55 °C upon immobilization. CLEA forms displayed lower Km value for AflAmy-IM, indicating increased substrate affinity. Reusability tests showed immobilised AflAmy retained activity over nine cycles, AflGlu over three, and their combined form (Combi-CLEA) up to nine cycles. Thermal stability of immobilised AflAmy improved, maintaining activity for 150 min at 70 °C. Both free and immobilized forms of the enzymes achieved ∼50 % starch hydrolysis within 60 min, demonstrating comparable catalytic efficiency and enhanced operational stability after immobilization.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"229 ","pages":"Article 110106"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146185399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platelet membrane-modified liquid metal nanoparticles enhanced cellular uptake and tumor photothermal therapy 血小板膜修饰的液态金属纳米颗粒增强细胞摄取和肿瘤光热治疗

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-05-01 Epub Date: 2026-02-03 DOI: 10.1016/j.bej.2026.110108

Siyuan Pang , Zhihang Wang , Yang Zou , Li Zhang , Yonggang Lv

{"title":"Platelet membrane-modified liquid metal nanoparticles enhanced cellular uptake and tumor photothermal therapy","authors":"Siyuan Pang , Zhihang Wang , Yang Zou , Li Zhang , Yonggang Lv","doi":"10.1016/j.bej.2026.110108","DOIUrl":"10.1016/j.bej.2026.110108","url":null,"abstract":"<div><div>Liquid metal (LM) nanoparticles have been widely used in photothermal therapy (PTT), but they are susceptible to oxidative inactivation and have poor targeting ability. Platelets (PLT) have many abundant membrane proteins on their surface that can be used to modify LM nanoparticles. Here, a gallium (Ga)-based LM-based nanoparticle delivery system was developed. The conducting polymer polypyrrole (PPy) was first grown <em>in situ</em> on the surface of LM nanoparticles by polymerization (named as LM@PPy). The oxidation resistance and photothermal stability of LM were improved. Subsequently, PLT membrane (PM) was extracted and coated on the surface of LM@PPy to prepare LM@PPy/PM. The antitumor effect of LM@PPy/PM was investigated through <em>in vitro</em> and <em>in vivo</em> experiments. It was demonstrated that the LM@PPy had better photothermal stability and their photothermal conversion efficiency reached 55 ± 2 %, which was higher than that of unmodified LM nanoparticles (31 ± 2 %). Most of the membrane proteins from PLT were retained on the prepared LM@PPy/PM. The PM coating effectively enhanced the tumor-targeting ability of the nanoparticles, leading to better tumor accumulation and antitumor effects in <em>in vitro</em> and <em>in vivo</em>. The findings showed that this nanoparticle delivery system provided a new technological solution to improve the antitumor ability of LM nanoparticles.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"229 ","pages":"Article 110108"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146185323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative study of biofilter and continuous stirred tank reactor for anammox fast start-up and stable operation 厌氧氨氧化生物滤池与连续搅拌槽反应器快速启动稳定运行的对比研究

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-05-01 Epub Date: 2026-02-09 DOI: 10.1016/j.bej.2026.110117

Longyan Chang , Dingying Ning , Mingyue Li , Wenqing Tian , Chu Wang , Fuxiong Nan , Di Wu , Jidong Liang , Wei Yan

{"title":"Comparative study of biofilter and continuous stirred tank reactor for anammox fast start-up and stable operation","authors":"Longyan Chang , Dingying Ning , Mingyue Li , Wenqing Tian , Chu Wang , Fuxiong Nan , Di Wu , Jidong Liang , Wei Yan","doi":"10.1016/j.bej.2026.110117","DOIUrl":"10.1016/j.bej.2026.110117","url":null,"abstract":"<div><div>The performance of anaerobic ammonium oxidation (anammox) in mainstream wastewater treatment is often limited by slow start-up and biomass retention challenges. In this study, biofilter (BF) and continuous stirred-tank reactor (CSTR) configurations were evaluated for anammox cultivation, focusing on start-up performance, nitrogen removal, and microbial dynamics. The results showed that the BF achieved higher total nitrogen removal rate (TNRE) of 79.35 % ± 0.63 % and nitrogen removal rate (NRR) of 1.35 ± 0.04 kg N/(m<sup>3</sup>·d) than 74.99 ± 3.53 % of TNRE and 0.59 ± 0.04 kg N/(m<sup>3</sup>·d) of NRR in CSTR. The nitrogen removal of BF driven by the combined contribution of anammox, DN and PN/A. CSTR achieved a shorter start-up time within 34 days and a higher anammox nitrogen removal contribution of 92.22 ± 4.50 % than 59 days for start-up and 84.65 ± 4.80 % anammox nitrogen removal contribution of BF, reflecting the predominance of the anammox pathway. BF showed vertical stratification of nitrogen removal, whereas CSTR formed stable granular sludge with high activity. Moreover, microbial analysis revealed distinct enrichment patterns, with <em>Candidatus Brocadia</em> dominating in BF and <em>Candidatus Kuenenia</em> in CSTR, reflecting niche differentiation driven by reactor configuration. These findings highlight the influence of reactor design on anammox activity, microbial community formation, and nitrogen removal efficiency, providing insights for optimizing mainstream anammox applications in wastewater treatment.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"229 ","pages":"Article 110117"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146185400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of fine-grained sulfur particles on sulfur autotrophic denitrification process: Evaluation of performance benefits, cold-tolerant mechanism and operational challenges 细粒硫颗粒在硫自养反硝化过程中的应用：性能效益评价、耐寒机制和操作挑战

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-05-01 Epub Date: 2026-02-10 DOI: 10.1016/j.bej.2026.110119

Mingdong Chang , Kuo Zhang , Tong Zhu , Chunli Zheng , Tangfu Xiao , Jianqiao Wang , Feng Ma , Zhitie Chang , Youzhao Wang

{"title":"Application of fine-grained sulfur particles on sulfur autotrophic denitrification process: Evaluation of performance benefits, cold-tolerant mechanism and operational challenges","authors":"Mingdong Chang , Kuo Zhang , Tong Zhu , Chunli Zheng , Tangfu Xiao , Jianqiao Wang , Feng Ma , Zhitie Chang , Youzhao Wang","doi":"10.1016/j.bej.2026.110119","DOIUrl":"10.1016/j.bej.2026.110119","url":null,"abstract":"<div><div>The poor sulfur bioavailability poses a key constraint on the practical application of sulfur autotrophic denitrification (SAD). This study employed fine-grained sulfur particles to address this limitation, evaluating their impact on denitrification performance, clogging risk, sulfur loss, and low-temperature adaptability. Results showed that fine particles significantly enhanced biomass accumulation and nitrate reductase content, improving system tolerance to loading shocks. Response surface methodology identified optimal conditions for the fine-particle reactor as a 1.5 h HRT, pH 7.0, and 0.5 mg/L dissolved oxygen concentration, achieving 82.8 % denitrification efficiency and a nitrogen removal rate of 0.473 kg/(m<sup>3</sup>·d). However, finer particles also increased the biomass growth rate by approximately 10 % and raised abiotic loss of sulfur particles by 84.8 %, indicating heightened risks of reactor clogging and sulfur washout. Microbial community analysis under low-temperature operation revealed that while SAD bacteria remained dominant, the increased content of extracellular polymeric substances (EPS) substantially elevated the abundance of endogenous heterotrophic denitrifiers. The protective role of EPS, combined with heterotrophic metabolic compensation, jointly supported stable nitrogen removal at low temperatures. In summary, despite associated challenges like clogging and sulfur loss, the fine-particle reactor demonstrated superior adaptability and operational stability under dynamic environmental and variable water quality conditions compared to conventional SAD filters. This offers a promising strategy for advancing the engineering application of SAD technology.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"229 ","pages":"Article 110119"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146185297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantifying the rate-limiting step and microbial stratification in linear alkylbenzene sulfonate biodegradation within a pilot-scale anaerobic-aerobic biofilm system 在中试厌氧-好氧生物膜系统中定量线性烷基苯磺酸生物降解的限速步骤和微生物分层

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-05-01 Epub Date: 2026-02-09 DOI: 10.1016/j.bej.2026.110115

Huiqing Lou , Xianzhong Cao

{"title":"Quantifying the rate-limiting step and microbial stratification in linear alkylbenzene sulfonate biodegradation within a pilot-scale anaerobic-aerobic biofilm system","authors":"Huiqing Lou , Xianzhong Cao","doi":"10.1016/j.bej.2026.110115","DOIUrl":"10.1016/j.bej.2026.110115","url":null,"abstract":"<div><div>The widespread discharge of linear alkylbenzene sulfonate (LAS) into wastewater poses significant challenges for conventional treatment systems. This study provides a quantitative, multi-level mechanistic investigation into LAS biodegradation within a novel pilot-scale anaerobic filter–biological contact oxidation (AF–BCO) stratified biofilm system. Under optimized conditions (total HRT 24 h, DO 4.0–5.0 mg/L, 30–35 ℃), the system achieved stable removal efficiencies of 98.2 % for LAS and 96.1 % for COD. Crucially, metabolic flux analysis quantitatively identified alkyl chain β-oxidation as the rate-limiting step, with a characteristic sulfonate hydrolysis-to-β-oxidation flux ratio of 3.2:1, providing a kinetic rationale for process design beyond empirical optimization. High-throughput sequencing and metagenomics revealed a spatially orchestrated metabolic division of labor: <em>Desulfobacter</em> (18.7 %) dominated the anaerobic biofilm, correlating with high <em>suyAB</em> gene abundance and sulfonatase activity for initial sulfonate cleavage, while <em>Pseudomonas</em> (22.1 %) prevailed in the aerobic biofilm, aligning with elevated <em>CYP450</em> gene expression and monooxygenase activity for ring mineralization. Kinetic modeling validated substrate and oxygen utilization patterns, and the system demonstrated remarkable resilience, fully recovering treatment efficiency within 48 h after a severe shock load (250 mg/L LAS). By integrating metabolic fluxes, microbial ecology, molecular biology, and reactor kinetics, this work establishes a predictive, mechanistic framework for the design of stratified biofilm systems treating surfactant-laden wastewater.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"229 ","pages":"Article 110115"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146185402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-pot preparation of magnetic cross-linked enzyme aggregates from a crude enzyme cocktail for cascaded catalytic production of biliverdin 用粗酶混合物一锅制备磁性交联酶聚集体用于级联催化生产胆绿素

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-05-01 Epub Date: 2026-02-12 DOI: 10.1016/j.bej.2026.110116

Xiyuan Tang , Meiying Zhu , Zhipeng Liu , Longyu Zhang , Chengli Yang , Jian Zhang , Zhaohui Liu , Dali Li

{"title":"One-pot preparation of magnetic cross-linked enzyme aggregates from a crude enzyme cocktail for cascaded catalytic production of biliverdin","authors":"Xiyuan Tang , Meiying Zhu , Zhipeng Liu , Longyu Zhang , Chengli Yang , Jian Zhang , Zhaohui Liu , Dali Li","doi":"10.1016/j.bej.2026.110116","DOIUrl":"10.1016/j.bej.2026.110116","url":null,"abstract":"<div><div>Immobilized enzyme technology has been widely used because of its advantages in enhancing enzyme stability and reusability. However, conventional methods rely on highly purified enzymes, which involve tedious extraction steps and high costs, thereby limiting their practical applications. In recent years, direct immobilization strategy based on crude enzyme has garnered attention due to its economy and high efficiency. This study developed a one-pot co-immobilization strategy where crude enzymes of recombinant cytochrome P450 reductase (rCPR), recombinant glucose dehydrogenase (rGDH), and recombinant heme oxygenase (rHO) were mixed with genipin and aminated magnetic nanoparticles to prepare magnetic cross-linked enzyme aggregates (MCLEAs) for the cascade catalysis of heme to biliverdin. After immobilization, the immobilization efficiency of rCPR, rGDH and rHO reached 64.3 %, 70.6 % and 95.2 %, respectively. The activity recovery of rCPR, rGDH, and rHO reached 105.0 %, 65.0 % and 120.0 %, respectively. The thermal and pH stability of the enzymes in MCLEAs were greatly improved, while excellent reusability and storage stability were achieved. After 7 catalytic cycles and 30 days of storage, the MCLEAs retained 50.0 % and 35.3 % of the residual activity, respectively. The cascade system significantly enhanced the catalytic performance of rHO, achieving a biliverdin production of 0.70 μM after 10 min of reaction. This study offers a novel strategy for the simple immobilization and efficient application of complex enzyme systems.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"229 ","pages":"Article 110116"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146185401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A first newly isolated strain capable of effectively degrading 3-methylpentane: Identification, degradation characteristics and pathway analysis 一个新分离的能有效降解3-甲基戊烷的菌株：鉴定、降解特性和途径分析

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-05-01 Epub Date: 2026-01-19 DOI: 10.1016/j.bej.2026.110084

Qian Li , Yujing Zhao , Liqin Sun , Zhonghui Sun , Raquel Lebrero , Raúl Muñoz , Jianmeng Chen , Rongsheng Lin , Dongzhi Chen

{"title":"A first newly isolated strain capable of effectively degrading 3-methylpentane: Identification, degradation characteristics and pathway analysis","authors":"Qian Li , Yujing Zhao , Liqin Sun , Zhonghui Sun , Raquel Lebrero , Raúl Muñoz , Jianmeng Chen , Rongsheng Lin , Dongzhi Chen","doi":"10.1016/j.bej.2026.110084","DOIUrl":"10.1016/j.bej.2026.110084","url":null,"abstract":"<div><div>Biotechnology is applicable for the treatment of volatile organic compounds emitted from petrochemical industries. However, the biodegradation of methyl alkanes is limited by low microbial activity and poor mass transfer. Herein, a novel strain capable of effectively utilizing 3-methylpentane (3MP) as the sole carbon source was firstly isolated and identified as <em>Mycobacterium</em> sp. SLQ-1. The strain could function well within wide ranges of pH (5.0–8.0) and temperature (30–45℃), with the maximum specific growth and biodegradation rates being 0.084 and 0.587 h<sup>−1</sup>, respectively. The optimum function conditions (pH 6.9, temperature 36.2℃) and 3MP removal efficiencies were predicted via response surface analysis. The repeated recycling of 2-methyl silicone oil enhanced 3MP biodegradation due to microbial adsorption. Two possible 3MP biodegradation pathways were proposed based on the analysis of metabolic intermediates, genes and enzymes. Furthermore, the strain had a wide utilization spectrum toward various alkanes, olefins and aromatics, presenting great engineering application potential.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"229 ","pages":"Article 110084"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146006642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Limit of hydrogen addition for enhanced methane concentration and production during syngas biomethanation in thermophilic trickle-bed reactor 嗜热滴床反应器合成气生物甲烷化过程中加氢提高甲烷浓度和产量的限制

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-05-01 Epub Date: 2026-02-05 DOI: 10.1016/j.bej.2026.110112

Florian Gabler , George Cheng , Leticia Pizzul , Anna Schnürer , Åke Nordberg

{"title":"Limit of hydrogen addition for enhanced methane concentration and production during syngas biomethanation in thermophilic trickle-bed reactor","authors":"Florian Gabler , George Cheng , Leticia Pizzul , Anna Schnürer , Åke Nordberg","doi":"10.1016/j.bej.2026.110112","DOIUrl":"10.1016/j.bej.2026.110112","url":null,"abstract":"<div><div>Syngas biomethanation is an emerging technology that converts synthesis gas, primarily composed of hydrogen (H<sub>2</sub>), carbon monoxide (CO), and carbon dioxide (CO<sub>2</sub>), into methane (CH<sub>4</sub>) through microbial activity. In this study, the effect of changing syngas composition with increased H<sub>2</sub> shares on CH<sub>4</sub> concentration and production was assessed for 125 days, using a thermophilic trickle-bed reactor (5 L). With the experimental upper limit of 71 % H<sub>2</sub> (14 % CO, 10 % CO<sub>2</sub>, 5 % N<sub>2</sub>) in the syngas, the maximum CH<sub>4</sub> concentration was 65 %, maintaining high methane evolution rates (4 L/(L<sub>pbv</sub>·d)) and high H<sub>2</sub> and CO conversion rates (>95 %). Targeted sulfur supplementation (Na<sub>2</sub>S) did not improve H<sub>2</sub> and CO conversion or CH<sub>4</sub> productivity, indicating that sulfur was no limiting factor under digestate-based operation. Reactor performance was instead constrained by system-level factors, including low gas retention time, gas–liquid mass transfer limitations, and inhibition of CO-converting pathways at elevated H<sub>2</sub> partial pressure. 16S rRNA gene sequencing revealed a highly stable microbial community dominated by the hydrogenotrophic methanogen <em>Methanothermobacter</em>. CO conversion occurred via direct methanogenesis and acetate formation, followed by syntrophic acetate oxidation. Overall, increasing H<sub>2</sub> availability enhanced CH<sub>4</sub> production only up to a system-specific threshold, beyond which microbial and transport limitations dominated.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"229 ","pages":"Article 110112"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146185321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0