Biochemical Engineering Journal最新文献_第3页

Simultaneous endogenous partial denitrification/anammox process for low-strength wastewater treatment: Process optimization, nitrogen removal and microbial dynamics 用于低浓度废水处理的同步内源部分反硝化/氨氧化工艺：工艺优化、脱氮和微生物动力学

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-11-09 DOI: 10.1016/j.bej.2024.109568

Dong Li , Fanxu Zeng , Songwei Yang , Yuliang Zhu , Zhu Li , Huiping Zeng , Jie Zhang

{"title":"Simultaneous endogenous partial denitrification/anammox process for low-strength wastewater treatment: Process optimization, nitrogen removal and microbial dynamics","authors":"Dong Li , Fanxu Zeng , Songwei Yang , Yuliang Zhu , Zhu Li , Huiping Zeng , Jie Zhang","doi":"10.1016/j.bej.2024.109568","DOIUrl":"10.1016/j.bej.2024.109568","url":null,"abstract":"<div><div>The endogenous partial denitrification process (EPD) led by glycogen-accumulating organisms (GAOs) has become an alternative to NO<sub>2</sub><sup>−</sup> supply in mainstream anaerobic-ammonia oxidation (anammox). However, low autotrophic nitrogen removal contribution is an urgent problem that needs to be solved in simultaneous endogenous partial denitrification/anammox (EPDA) system. This study used anaerobic duration optimization to enhance the autotrophic nitrogen removal capacity of EPDA system. The results showed that the EPDA activity increased from 0.67 to 1.09 mg N/g VSS·h after anaerobic time was extended to 120 min. This significantly improved the contribution of anammox to TIN removal, increasing from 23.5 % to 61.6 %. During the phase Ⅲ, Eff.TIN of 4.5±1.8 mg/L and NRE of 92.2 %±3.0 %. The enrichment of AnAOB (<em>Candidatus Brocadia</em>) and GAOs (<em>Defluviicoccus</em>) was responsible for maintaining the stability of the EPDA process. This study provides a feasible optimization strategy for improving the contribution of autotrophic nitrogen removal in the EPDA system.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109568"},"PeriodicalIF":3.7,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of deep eutectic solvent pre-treated natural rubber gloves by Klebsiella aerogenes: A sustainable approach to rubber waste management 产气克雷伯氏菌对深共晶溶剂预处理天然橡胶手套的生物降解：橡胶废物管理的可持续方法

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-11-08 DOI: 10.1016/j.bej.2024.109569

Harika Chittella , Li Wan Yoon , Suganti Ramarad , Zee-Wei Lai

{"title":"Biodegradation of deep eutectic solvent pre-treated natural rubber gloves by Klebsiella aerogenes: A sustainable approach to rubber waste management","authors":"Harika Chittella , Li Wan Yoon , Suganti Ramarad , Zee-Wei Lai","doi":"10.1016/j.bej.2024.109569","DOIUrl":"10.1016/j.bej.2024.109569","url":null,"abstract":"<div><div>This study investigates the potential of deep eutectic solvents (DES) to enhance the biodegradation of natural rubber gloves (NRG) by <em>Klebsiella aerogenes</em>. Choline chloride and urea (ChCl: urea) was the DES employed to pre-treat NRG at various temperatures (80°C to 140°C) and durations (0.5 h to 5 h). Pre-treated rubber (p-NRG) underwent significant physical and chemical changes, enhancing its biodegradability. Analytical techniques such as dry weight analysis, bacteria cell concentration, FTIR TGA, and SEM were used to characterize the pre-treated and biodegraded samples. The results have demonstrated a significant weight loss and structural modifications in p-NRG, with the highest degradation of 43 % observed at 140°C for 5 hours of pretreatment before biodegradation. Meanwhile, merely 17 % of weight loss was observed when pre-treatment was not employed. DES pre-treatment notably enhanced NRG biodegradability, achieving a 50.6 % weight loss when biodegradation was conducted at pH 7 and 35°C. The highest cell concentration, 0.75 g/L, was recorded in the second week of the biodegradation process. Results have indicated that the maximum protein concentration of 697.3 µg/ml, along with the highest enzyme activities for laccase and manganese peroxidase (MnP) at 0.46 ± 0.05 IU and 0.30 ± 0.05 IU respectively, were recorded in the second week of the biodegradation process. DES pre-treatment has significantly improved the biodegradability of NRG by <em>Klebsiella aerogenes</em>, offering a promising and eco-friendly solution for rubber waste management.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109569"},"PeriodicalIF":3.7,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of 3D tumor spheroid maturity on cell migration and invasion dynamics 三维肿瘤球体成熟度对细胞迁移和侵袭动力学的影响

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-11-08 DOI: 10.1016/j.bej.2024.109567

Lingke Feng , Rong Pan , Ke Ning , Wen Sun , Yirong Chen , Yuanyuan Xie , Mingzhu Wang , Yan Li , Ling Yu

{"title":"The impact of 3D tumor spheroid maturity on cell migration and invasion dynamics","authors":"Lingke Feng , Rong Pan , Ke Ning , Wen Sun , Yirong Chen , Yuanyuan Xie , Mingzhu Wang , Yan Li , Ling Yu","doi":"10.1016/j.bej.2024.109567","DOIUrl":"10.1016/j.bej.2024.109567","url":null,"abstract":"<div><div>Cell motility is crucial in cancer metastasis, and understanding its regulation in tumor cells is vital for developing anti-metastatic therapies. Traditional 2D cell culture assays provide insights into cell migration but fail to replicate the complex 3D architecture of tissues <em>in vivo</em>. 3D cell culture models like tumor spheroids have been applied for cell migration tests. This study investigates the role of spheroid maturity in tumor cell motility, hypothesizing that spheroid maturity mirrors physiological conditions in solid tumors. Human prostate (DU 145), breast (MCF-7), and murine breast (EMT-6) cancer cells were cultured into spheroids of varying time (3, 7, and 11 days). The migration and invasion of these spheroids were analyzed, revealing that 11-day-old DU 145 spheroids demonstrated the greatest horizontal migration, correlating with RNA-seq data showing increased cell adhesion, cytoskeleton dynamics, and motility pathways. Confocal microscopy and single-cell multimode analyzer indicated higher reactive oxygen species (ROS) levels in mature spheroids, potentially activating motility pathways. Additionally, DU 145 spheroids were treated with chemotherapy reagent Doxorubicin (DOX), and the results showed that spheroids culture for 7 and 11 days exhibited greater resistance to DOX compared to spheroids cultured for 3 days. These findings highlighted the importance of considering spheroid maturity in cancer research and drug development, emphasizing the need for systematic analysis of spheroid growth conditions to ensure reproducible and reliable experimental settings.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109567"},"PeriodicalIF":3.7,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced anaerobic digestion by co-immobilization of anaerobic microbes and conductive particles in hydrogel for enhanced methane production performance 通过将厌氧微生物和导电颗粒共同固定在水凝胶中实现高级厌氧消化，从而提高甲烷生产性能

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-11-08 DOI: 10.1016/j.bej.2024.109563

Stella Chan , Kento Nishi , Mitsuhiko Koyama , Tatsushi Matsuyama , Junichi Ida

{"title":"Advanced anaerobic digestion by co-immobilization of anaerobic microbes and conductive particles in hydrogel for enhanced methane production performance","authors":"Stella Chan , Kento Nishi , Mitsuhiko Koyama , Tatsushi Matsuyama , Junichi Ida","doi":"10.1016/j.bej.2024.109563","DOIUrl":"10.1016/j.bej.2024.109563","url":null,"abstract":"<div><div>Recent research has increasingly focused on the enhancement of anaerobic digestion (AD) through direct interspecies electron transfer (DIET) facilitated by conductive particles (CP). Although this approach can significantly accelerate the AD process, the contact efficiency between CPs and AD microbes is relatively low due to the flow of water in a dispersed condition, leading to possible DIET inefficiency. In this study, a unique approach involving the “co-immobilization” of anaerobic microbes and multi-walled carbon nanotubes (MWCNTs) as CP into a hydrogel matrix was developed to improve the AD process. The advantages of this method include improved contact efficiency between microbes and CPs for enhanced DIET, and increased CP retention within the reactor, thereby omitting the need to compensate for CP washout. The methane production rate for the co-immobilized hydrogel was 2.5-fold and 1.9-fold faster than that of the control (dispersed sludge) and conventional DIET (dispersed sludge with MWCNT addition), respectively. Microbial analysis indicated the enrichment of functional microbes such as <em>Anaerolineacea</em>, <em>Sedimentibacteraceae, Rhodocyclaceae,</em> and <em>Methanothrichaceae,</em> which could be involved in the DIET under co-immobilized conditions. These results demonstrate the potential of the proposed method for realizing an advanced continuous AD process through improved DIET.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109563"},"PeriodicalIF":3.7,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing an off-site bicarbonation absorber system to promote microalgal fixation of CO2 in exhaust gas from biogas upgrading 开发异地碳酸氢盐吸收系统，促进微藻固定沼气提纯废气中的二氧化碳

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-11-08 DOI: 10.1016/j.bej.2024.109558

Yulun Wu , Zhao Li , Xin Wang , Zaiyin Yu , Weiguang Mao , Cai Cheng , Guanmou Che , Jun Cheng

{"title":"Developing an off-site bicarbonation absorber system to promote microalgal fixation of CO2 in exhaust gas from biogas upgrading","authors":"Yulun Wu , Zhao Li , Xin Wang , Zaiyin Yu , Weiguang Mao , Cai Cheng , Guanmou Che , Jun Cheng","doi":"10.1016/j.bej.2024.109558","DOIUrl":"10.1016/j.bej.2024.109558","url":null,"abstract":"<div><div>In order to address the risk of explosion due to CH<sub>4</sub> from exhaust gas produced during biogas upgrading in closed carbon fixation systems employing photosynthetic microalgae, an off-site bicarbonation absorber system was developed to promote microalgal CO<sub>2</sub> fixation under atmospheric pressure. The abundant CO<sub>2</sub> in the biogas upgrading exhaust gas (≥90 vol% CO<sub>2</sub>, ≤10 vol% CH<sub>4</sub>) reacted with a Na<sub>2</sub>CO<sub>3</sub> solution in the off-site bicarbonation absorber to produce NaHCO<sub>3</sub>, which was used as carbon source for microalgal growth in enclosed column photobioreactors. After the reaction, CH<sub>4</sub> was discharged outside the bicarbonation absorber because it did not react with the Na<sub>2</sub>CO<sub>3</sub> solution and was extremely difficult to dissolve in water, thereby avoiding the explosion risk due to accumulated CH<sub>4</sub> in the enclosed column photobioreactors. The experimental results showed that the <em>Spirulina</em> growth rate first increased 1.7 times, peaking at 0.6 g/L/d, and then decreased when the bicarbonation reaction time (optimal 50 min), absorber diameter (optimal 10 cm), initial Na<sub>2</sub>CO<sub>3</sub> concentration (optimal 173 mM), and exhaust gas aeration rate (optimal 100 sccm) increased. The optimal molar ratio of NaHCO<sub>3</sub> to total inorganic carbon in the bicarbonation absorber solution reached 79 %. The sufficient HCO<sub>3</sub><sup>-</sup> supply and suitable pH of the microalgal solution improved the synthesis of photosynthetic pigments in the microalgal cells and enhanced their photochemical efficiency and carbon sequestration rates.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109558"},"PeriodicalIF":3.7,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel tubular single-chamber microbial electrolysis cell for efficient methane production from industrial potato starch wastewater 从工业马铃薯淀粉废水中高效生产甲烷的新型管式单室微生物电解池

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-11-07 DOI: 10.1016/j.bej.2024.109561

Gaoyuang Shang , Jinpeng Yu , Kai Cui , Hong Zhang , Yuhan Guo , Menglong Zhao , Chengjun Wang , Kun Guo

{"title":"A novel tubular single-chamber microbial electrolysis cell for efficient methane production from industrial potato starch wastewater","authors":"Gaoyuang Shang , Jinpeng Yu , Kai Cui , Hong Zhang , Yuhan Guo , Menglong Zhao , Chengjun Wang , Kun Guo","doi":"10.1016/j.bej.2024.109561","DOIUrl":"10.1016/j.bej.2024.109561","url":null,"abstract":"<div><div>The integration of microbial electrolysis cells (MEC) with anaerobic digestion (AD) shows great promise for enhancing methane production from high-COD wastewater. However, an efficient MEC-AD reactor design remains elusive. Here, a novel tubular single-chamber MEC-AD reactor was constructed to treat potato starch wastewater (COD over 20,000 mg/L). The concentric and compact design of the stainless-steel cathode and anode reduced internal resistance, resulting in enhanced methane production. Applying −0.2 V vs. Ag/AgCl to the anode increased methane production by 1.73 times compared to the open circuit and halved hydraulic retention time. Moreover, the reactor achieved an average methane content of 82.57 %, which was 23.89 % higher than the open circuit. The reactor showed a total COD removal of 92.2 %, which was 24 % higher than the open circuit. Additionally, base consumption to maintain pH was reduced to one-sixth of that in conventional AD, preventing volatile fatty acid accumulation. Microbial analysis showed <em>Geobacter</em> (63.4 %) and <em>Methanobacterium</em> (96.8 %) were highly enriched in the anode and cathode biofilms, respectively. The proportion of fermentative bacteria also increased in the MEC-AD system. These results demonstrate the effectiveness of the tubular single-chamber MEC-AD reactor in enhancing methane production from potato starch wastewater, with strong potential for scale-up applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109561"},"PeriodicalIF":3.7,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improvement of sludge dewatering performance by persulfate advanced oxidation combined with LDH: Synergistic effect of free radical and non-free radical and reuse of deep-dewatered sludge cake 过硫酸盐高级氧化结合 LDH 改善污泥脱水性能：自由基和非自由基的协同效应以及深度脱水污泥饼的再利用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-11-07 DOI: 10.1016/j.bej.2024.109560

Jingyu Lv, Lei Song, Yang Guo

{"title":"Improvement of sludge dewatering performance by persulfate advanced oxidation combined with LDH: Synergistic effect of free radical and non-free radical and reuse of deep-dewatered sludge cake","authors":"Jingyu Lv, Lei Song, Yang Guo","doi":"10.1016/j.bej.2024.109560","DOIUrl":"10.1016/j.bej.2024.109560","url":null,"abstract":"<div><div>The high-water content of sludge in wastewater plant will influence the transportation and utilization. In this study, a new method for improving sludge dewatering by pyrite (FeS<sub>2</sub>) activated persulfate (PMS) combined with layered double hydroxide (LDH) was proposed. After conditioning, the water content (Wc) and specific resistance (SRF) of sludge decreased from 97.12 % and 1.83 × 10<sup>13</sup> m/kg to 71.39 % and 1.84 × 10<sup>12</sup> m/kg, severally. SEM and particle size analysis showed the system could destroy sludge cells effectively.The mechanism analysis of protein and polysaccharide content, 3D-EEM, FTIR, XPS results showed that FeS<sub>2</sub>/PMS-LDH combined system was beneficial to break down the sludge extracellular polymer (EPS), transform and accumulate the organic matter into the EPS outer layer, release the bound water. Both free radical and non-free radical play a role in oxidation, and they cooperate to break EPS. The effective phosphate adsorption performance of the biochar adsorbent prepared from dehydrated sludge cake was also investigated. The adsorption behavior of phosphate on biochar from dewatered sludge cake belongs to uniform chemical monolayer adsorption. When T = 298k, PH = 5, the maximum adsorption capacity is 20.255 mg/g. The introduction of LDH is helpful to enhance the sludge dewatering and the adsorption of phosphate. To sum up, the combined conditioning method considers the effectiveness of sludge dewatering and the feasibility of sludge cake disposal and utilization.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109560"},"PeriodicalIF":3.7,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable remediation of piggery wastewater using a novel mixotrophic Chlorella sorokiniana Cbeo for high value biomass production 利用新型混养小球藻 Cbeo 对养猪场废水进行可持续修复，以生产高价值生物质

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-11-06 DOI: 10.1016/j.bej.2024.109555

Do Thi Cam Van , Dang Thi Mai , Bui Thi Thu Uyen , Nguyen Thi Phuong Dung , Lu Thi Thu Ha , Nguyen Thi Lieu , Dang Nhat Minh , Tran Dang Thuan , Le Truong Giang

{"title":"Sustainable remediation of piggery wastewater using a novel mixotrophic Chlorella sorokiniana Cbeo for high value biomass production","authors":"Do Thi Cam Van , Dang Thi Mai , Bui Thi Thu Uyen , Nguyen Thi Phuong Dung , Lu Thi Thu Ha , Nguyen Thi Lieu , Dang Nhat Minh , Tran Dang Thuan , Le Truong Giang","doi":"10.1016/j.bej.2024.109555","DOIUrl":"10.1016/j.bej.2024.109555","url":null,"abstract":"<div><div>Piggery wastewater (PW) contains high density of organic carbon (COD), nitrogen (NH<sub>4</sub><sup>+</sup>-N and TN) and phosphorous (TP), which are essential nutrients for microalgae growth. This work was attempted to use a newly isolate <em>Chlorella sorokiniana C</em><sub><em>beo</em></sub> for recovery these compounds into its biomass via mixotrophic cultivation. Critical factors including level of ammonia, C/N ratio, pH, light intensity, sterilized/unsterilized media, and indoor/outdoor cultivations affecting biomass production and nutrients removal efficiencies were investigated. Data revealed that <em>C</em>. <em>sorokiniana C</em><sub><em>beo</em></sub> achieved the optimal growth in the unsterilized medium at NH<sub>4</sub><sup>+</sup>-N concentration, C/N ratio, initial pH, and light intensity of 250 mg/L, 10/1, 7, and 150 μmol/m<sup>2</sup>/s, respectively. Under the optimal conditions, dry cell weight (DCW) reached the maximal level of 4.30 g/L, which was slightly higher than 4.14 g/L determined for the sterilized medium. In 30 L-scale photobioreactor, <em>C. sorokiniana C</em><sub><em>beo</em></sub> grown under indoor and outdoor achieved DCW of 3.61 and 3.19 g/L, respectively. COD, NH<sub>4</sub><sup>+</sup>-N, TN, TP removal efficiencies for both conditions were determined as 91.9–96.7, 96.6–99.7, 96.2–96.4, and 98.2–100 %, respectively. The <em>C. sorokiniana C</em><sub><em>beo</em></sub> biomass contained 14–27 % lipid, 25–32 % carbohydrate, 44–48 % protein, and 0.25–0.97 % lutein. Interestingly, α-Linolenic acid (C18:3n3) was 19.84 –27.0 % of the total fatty acids. <em>C. sorokiniana C</em><sub><em>beo</em></sub> is the promising algal strain for development of a sustainable biorefinery of PW.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109555"},"PeriodicalIF":3.7,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental determination of maximum shear stress in Mobius® Breez perfusion microbioreactors and comparative analysis with stirred tank bioreactors Mobius® Breez 灌注微型生物反应器中最大剪切应力的实验测定以及与搅拌罐生物反应器的比较分析

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-11-06 DOI: 10.1016/j.bej.2024.109556

Patrick Romann , Dan Trunov , Ondřej Šrom , Harry L.T. Lee , Kevin S. Lee , Ryan Trocki , David Ephraim , Jean-Marc Bielser , Jonathan Souquet , Miroslav Šoóš , Thomas K. Villiger

{"title":"Experimental determination of maximum shear stress in Mobius® Breez perfusion microbioreactors and comparative analysis with stirred tank bioreactors","authors":"Patrick Romann , Dan Trunov , Ondřej Šrom , Harry L.T. Lee , Kevin S. Lee , Ryan Trocki , David Ephraim , Jean-Marc Bielser , Jonathan Souquet , Miroslav Šoóš , Thomas K. Villiger","doi":"10.1016/j.bej.2024.109556","DOIUrl":"10.1016/j.bej.2024.109556","url":null,"abstract":"<div><div>Perfusion processes have experienced increased popularity in recent years due to their ability to sustain high cell densities and productivities in biopharmaceutical production, offering advantages over traditional batch and fed-batch cultivation methods. The Mobius® Breez microbioreactor significantly reduces experimental effort by downsizing the classical volume of perfusion bioreactors to the mL range and thus represents a valuable tool for process development. However, miniaturization has raised questions regarding comparability with traditional bioreactors in terms of the physical environment, such as hydrodynamic shear stress. Therefore, the maximum hydrodynamic shear stress, cultivation performance, and membrane-wall contact were evaluated to elucidate the system's behavior. Findings reveal two distinct operational conditions, distinguished by the presence or absence of membrane-wall contact, resulting in varying levels of hydrodynamic stress. Conditions lacking membrane contact demonstrate stress levels within safe operating thresholds for CHO cells, while those involving membrane contact exceed these thresholds, potentially leading to cell damage. Through the identification of critical frequencies of membrane motion, this study offers insights for optimizing microbioreactor operation and enhancing overall bioprocess efficiency.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109556"},"PeriodicalIF":3.7,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of inducible packaging cell line for rAAV production via CRISPR-Cas9 mediated site-specific integration 通过 CRISPR-Cas9 介导的位点特异性整合，开发用于生产 rAAV 的可诱导包装细胞系

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-31 DOI: 10.1016/j.bej.2024.109552

Qiang Fu , Yongdan Wang , Emily Doleh , Mark Blenner , Seongkyu Yoon

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