Biochemical Engineering Journal最新文献_第3页

Structural design of cellulose derivative-modified chitosan adsorbents for arsenic removal: Machine learning modeling, Box-Behnken design, Optuna hyperparameter tuning, and molecular dynamics 用于除砷的纤维素衍生物修饰壳聚糖吸附剂的结构设计：机器学习建模，Box-Behnken设计，Optuna超参数调整和分子动力学

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-20 DOI: 10.1016/j.bej.2025.109800

Mohammad Khajavian , Suzylawati Ismail , Javad Esmaeili

{"title":"Structural design of cellulose derivative-modified chitosan adsorbents for arsenic removal: Machine learning modeling, Box-Behnken design, Optuna hyperparameter tuning, and molecular dynamics","authors":"Mohammad Khajavian , Suzylawati Ismail , Javad Esmaeili","doi":"10.1016/j.bej.2025.109800","DOIUrl":"10.1016/j.bej.2025.109800","url":null,"abstract":"<div><div>Heavy metal contamination poses significant environmental and public health risks, necessitating the development of effective remediation strategies. Although cellulose derivatives are widely used to modify adsorbents, hydroxypropyl cellulose (HPC) has not yet been investigated for functionalizing adsorbents in heavy metal removal. This study aimed to enhance As(III) adsorption performance by optimizing the structural properties of the hydroxypropyl cellulose-modified chitosan/polyvinyl alcohol (HPC/CP) adsorbent using the Box-Behnken design (BBD) and several machine learning (ML) algorithms. The optimization process focused on four key structural parameters: chitosan (CS) content, HPC content, polyvinyl alcohol (PVA) content, and the thickness of the adsorbent. The random forest (RF) ML algorithm confirmed the highest predictive accuracy (R² = 0.99, MSE = 2.31) after hyperparameter tuning (max_depth = 38, min_samples_leaf = 29, min_samples_split = 13, n_estimators = 470). The optimal adsorbent composition was identified as 5 wt% CS, 4 wt% HPC, 12 wt% PVA, and 300 µm thickness. The optimized adsorbent achieved an experimental As(III) removal efficiency of 98.6 %, significantly higher than the unoptimized adsorbent (65.3 %). The RF-predicted removal (96.1 %) closely aligned with experimental results and outperformed other ML models. Molecular dynamics simulations further validated the structural stability of the As(III)/HPC/CP complex through reduced energy fluctuations. HPC functionalization, combined with ML-driven optimization, significantly enhances the As(III) removal efficiency of CS-based adsorbents.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109800"},"PeriodicalIF":3.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144106777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fermentative and physiological responses of Saccharomyces cerevisiae LMQA SRC-143 and PE-2 in co-culture with Lactobacillus fermentum: Insights with flow cytometry in the Melle-Boinot process 酿酒酵母LMQA SRC-143和PE-2与发酵乳杆菌共培养的发酵和生理反应：用流式细胞术观察Melle-Boinot过程

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-19 DOI: 10.1016/j.bej.2025.109799

Ramon P. Brexó , Fernando Pradella , Rafael D. Chaves , Antonio A. Câmara Jr , Dionisio P. Amorim-Neto , Alessandro S. Farias , Anderson S. Sant’Ana

{"title":"Fermentative and physiological responses of Saccharomyces cerevisiae LMQA SRC-143 and PE-2 in co-culture with Lactobacillus fermentum: Insights with flow cytometry in the Melle-Boinot process","authors":"Ramon P. Brexó , Fernando Pradella , Rafael D. Chaves , Antonio A. Câmara Jr , Dionisio P. Amorim-Neto , Alessandro S. Farias , Anderson S. Sant’Ana","doi":"10.1016/j.bej.2025.109799","DOIUrl":"10.1016/j.bej.2025.109799","url":null,"abstract":"<div><div>The presence of multiple microbial species during the fermentation process remains a major challenge for controlled and high-yield ethanol production. Therefore, it is essential to select strains capable of maintaining or enhancing their biotechnological activity when involved in interspecies interactions. This study aimed to assess the impact of microbial competition, mediated by <em>Lactobacillus fermentum</em> CCT 1668, along with cell recycling and acid washing, on the fermentative performance and physiological responses of <em>Saccharomyces cerevisiae</em> strains LMQA SRC-143 and PE-2. Flow cytometry was employed to assess cell vitality, viability, and intracellular carbohydrate reserves throughout the fermentation cycles. The results challenge the conventional notion that lactic acid bacteria co-culture is universally detrimental during fermentation. Interestingly, while PE-2 exhibited higher resilience when isolated, the presence of <em>L. fermentum</em> significantly enhanced the performance and vitality of LMQA SRC-143. This research underscores the potential of exploiting microbial interactions to optimize fermentation processes, using as an example the bioethanol production, suggesting that certain non-commercial yeast strains may not only withstand bacterial co-culture but also be benefited under specific conditions. These insights carry significant implications for the fermentation industry, as they could reduce dependency on chemical interventions for bacterial control, promoting more sustainable approaches aligned with SDG 7 - affordable and clean energy.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109799"},"PeriodicalIF":3.7,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144098968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating integration and pretreatment options, for first- and second-generation bioethanol production by adjacent sugarcane mills 对相邻甘蔗厂生产第一代和第二代生物乙醇的整合和预处理方案进行评估

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-19 DOI: 10.1016/j.bej.2025.109796

Farai Chireshe, Abdul M. Petersen, Johann F. Görgens

{"title":"Evaluating integration and pretreatment options, for first- and second-generation bioethanol production by adjacent sugarcane mills","authors":"Farai Chireshe, Abdul M. Petersen, Johann F. Görgens","doi":"10.1016/j.bej.2025.109796","DOIUrl":"10.1016/j.bej.2025.109796","url":null,"abstract":"<div><div>Integrated first- and second-generation (1G2G) bioethanol production from sugarcane molasses and lignocelluloses offers an economic and environmentally viable pathway to support decarbonisation of the transport and chemical sectors. The techno-economic performance of on-season versus year-round operation of a sugarcane biorefinery, leveraging the economies of scale benefits of using feedstock supplied by adjacent mills were assessed. Five different 1G2G configurations, varying in terms of sugar utilisation strategies, pretreatment technologies and inter-mill feedstock logistics were considered. Adopting year-round operation reduced the minimum ethanol selling price (MESP) by 11 %, compared to on-season only operation due to improved capital utilisation. Despite having the lowest MESP (1.16 US$/L), lignocelluloses storage for off-season use was deemed impractical due to biomass storage space requirements. Adding feedstock from one adjacent mill reduced MESPs by 11–22 %, and by 32 % for integration with 2 mills when only processing C5 sugars at host-mill. Liquid hot water (LHW) pretreatment coupled with hot water recycling and co-fermentation of C5 and C6 sugars was the most cost-effective and practical scenario (MESP = 1.25 US$/L). Hot water recycling was effective in reducing the energy (1.2 GJ/tonne biomass) and water demand (less 50 %). Doubling solids loading in LHW pretreatment reduced the MESP by over 11 %, demonstrating its potential for process intensification. Unlike previous studies, the benefits of regional mill integration were quantified.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109796"},"PeriodicalIF":3.7,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144098966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated waste-to-energy process for hazardous landfill leachate valorization: Economic profits related to sustainability and environmental assessment 危险垃圾填埋场渗滤液增值的综合废物发电过程：与可持续性和环境评估有关的经济利润

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-19 DOI: 10.1016/j.bej.2025.109797

Ahmed Tawfik , Nawaf S. Alhajeri , Mahmoud Nasr

{"title":"Integrated waste-to-energy process for hazardous landfill leachate valorization: Economic profits related to sustainability and environmental assessment","authors":"Ahmed Tawfik , Nawaf S. Alhajeri , Mahmoud Nasr","doi":"10.1016/j.bej.2025.109797","DOIUrl":"10.1016/j.bej.2025.109797","url":null,"abstract":"<div><div>While recent studies have presented hazardous landfill leachate (HLL) bio-digestion for biogas recovery, there is a research gap in enhancing the relative abundance of methanogens and managing the sludge digestate to avoid the risk of secondary pollution. This study represents the operation of an anaerobic digester amended with sawdust biochar to improve the HLL detoxification performance, followed by managing the sludge digestate to raise the project’s internal rate of return (IRR). This objective is justified by employing an integrated techno-financial and life cycle assessment (LCA) approach. Implementing the biochar-supplemented digester for HLL’s substrate conversion into biofuel achieved the shortest payback period (6.2 yr), considering biochar carbon credit (0.231 USD), biogenic gas selling (0.312 USD), biogas carbon marketing (0.118 USD), pollutant shadow price (0.064 USD), and digestate biochar selling (0.080 USD) per 1 m<sup>3</sup>. Increasing the <em>Methanosaeta</em>, <em>Methylophilus</em>, <em>Paludibacter</em>, <em>Novosphingobium</em>, and <em>Pseudomonas</em> relative abundances by 61.8 %, 529.3 %, 261.8 %, 25.9 %, and 100.0 %, respectively, enhanced the 2, 6-dichlorophenol dechlorination performance in the biochar-amendment digester. This project also maintained LCA endpoint impact categories of <img>6.05 × 10<sup><img>8</sup> species·yr, <img>8.11 × 10<sup><img>5</sup> DALYs, and 7.99 × 10<sup><img>2</sup> USD 2013 for ecosystem quality, human health, and natural resources, respectively. Because the proposed project would sustain an IRR of 9.7 %, and conserve terrestrial/aquatic ecosystems, future studies should focus on replacing fossil-based fuel with bio-CH<sub>4</sub> to mitigate the effects of human-induced climate change.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109797"},"PeriodicalIF":3.7,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144098967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced lipid production in Chlorella vulgaris via indole-3-acetic acid salt stress in a two-stage culture for biofuels 二阶段生物燃料培养中吲哚-3-乙酸盐胁迫下普通小球藻脂质生产的增强

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-16 DOI: 10.1016/j.bej.2025.109795

Qun Wei , Dan Zhao , Ming Wang , Conghan Wang , Fu Pang , Xiangmeng Ma

{"title":"Enhanced lipid production in Chlorella vulgaris via indole-3-acetic acid salt stress in a two-stage culture for biofuels","authors":"Qun Wei , Dan Zhao , Ming Wang , Conghan Wang , Fu Pang , Xiangmeng Ma","doi":"10.1016/j.bej.2025.109795","DOIUrl":"10.1016/j.bej.2025.109795","url":null,"abstract":"<div><div>Utilizing saline wastewater for the cultivation of microalgae represents a viable approach to decrease the production costs associated with microalgae-derived biodiesel. In this study, a new method of combining plant hormones with two-stage culture was proposed to drive efficient lipid production of microalgae under salt stress. The findings indicated that the introduction of 40 mg·L<sup>−1</sup> of indole-3-acetic acid (IAA) during the initial phase resulted in optimal cell density and lipid productivity of <em>Chlorella vulgaris</em> under conditions of 10 g·L<sup>−1</sup> NaCl stress, achieving values of 65.15 × 10<sup>6</sup> cells·mL<sup>−1</sup> and 47.62 mg·L<sup>−1</sup>·d<sup>−1</sup>, respectively. In the second stage, the application of salt stress was repeated, leading to a lipid productivity of 56.17 mg·L<sup>−1</sup>·d<sup>−1</sup>, which represents an increase of 150.8 % compared to the untreated control group. Additionally, the proportion of saturated fatty acids rose to 29.26 %. Therefore, it has better combustion characteristics and stability. At the same time, the self-flocculation efficiency of microalgae also increased by 93.75 %. In addition, the regulatory mechanism of IAA on the growth and lipid accumulation of <em>Chlorella</em> under salt stress was discussed by quantitative analysis of the expression of genes related to signaling pathways and lipid biosynthesis in algal cells. This study provides ideas for microalgae to produce biodiesel and achieve sustainable development.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109795"},"PeriodicalIF":3.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-purpose immobilization of lipase onto modified magnetic nanoparticles: Characterization and enhancement of enzymatic interesterification 改良磁性纳米颗粒固定化脂肪酶的多用途研究：酶催化活性的表征和增强

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-16 DOI: 10.1016/j.bej.2025.109794

Hui Fang , Yuanyuan Deng , Zhihao Zhao, Jiarui Zeng, Mingwei Zhang, Pengfei Zhou

{"title":"Multi-purpose immobilization of lipase onto modified magnetic nanoparticles: Characterization and enhancement of enzymatic interesterification","authors":"Hui Fang , Yuanyuan Deng , Zhihao Zhao, Jiarui Zeng, Mingwei Zhang, Pengfei Zhou","doi":"10.1016/j.bej.2025.109794","DOIUrl":"10.1016/j.bej.2025.109794","url":null,"abstract":"<div><div>Improving catalytic performance represents a highly desirable but challenging objective for the immobilization of biocatalysts. A novel biocatalyst of ANL@Fe<sub>3</sub>O<sub>4</sub>-SiO<sub>2</sub>-APTES was fabricated by immobilizing <em>Aspergillus niger</em> lipase (ANL) onto amino-alkyl group-modified magnetic nanoparticles. This biocatalyst exhibited an enzyme loading of 127.68 mg/g and the specific activity of 1132.26 U/g. The superior activity and recyclability of ANL@Fe<sub>3</sub>O<sub>4</sub>-SiO<sub>2</sub>-APTES was validated. The outstanding performance for the enzymatic interesterification (EIE) of palm stearin (PS) and rice bran oils (RBO) was observed. The significantly decreased the solid fat content (SFC) and enhanced crystallization rate of oils was achieved, as well as the β' type of oils after EIE was obtained. Moreover, molecular dynamics simulations were conducted to elucidate the underlying catalytic mechanism of EIE. This study proposes a highly efficient and sustainable approach for the chemical modification of lipids, thereby promoting its broad application in the food industry.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109794"},"PeriodicalIF":3.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144088826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical isolation of breast cancer Circulating Tumor Cells with an inertial microfluidic chip with a trapezoidal cross-section 用梯形惯性微流控芯片临床分离乳腺癌循环肿瘤细胞

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-16 DOI: 10.1016/j.bej.2025.109788

Zahra Sadeghi , Mohsen Nasr Esfahany , Hossein Salehi , Azar Baradaran

{"title":"Clinical isolation of breast cancer Circulating Tumor Cells with an inertial microfluidic chip with a trapezoidal cross-section","authors":"Zahra Sadeghi , Mohsen Nasr Esfahany , Hossein Salehi , Azar Baradaran","doi":"10.1016/j.bej.2025.109788","DOIUrl":"10.1016/j.bej.2025.109788","url":null,"abstract":"<div><div>In recent years, various detection and isolation methods for breast cancer have emerged. However, the lack of simple, unbiased separation techniques limits clinical applications of Circulating Tumor Cells (CTCs). This study introduces an inertial microfluidic chip with a trapezoidal cross-section for rapid, simple separation of MCF-7 CTCs from patient blood using an optimized SLA 3D printing technique. Simulations and experiments showed that increasing the slant angle enhances Dean vortices, with 1.2 mL/min as the optimal flow rate. Spiking MCF-7 cells into Phosphate-Buffered Saline (PBS) yielded a recovery rate of 88 ± 3.5 % and a purity of 99.99 %. When spiked into 7.5 mL lysed blood, the recovery was 86 ± 2.9 % and purity 94 ± 2.3 %, with over 90 % of cells remaining viable. Clinically, the chip detected MCF-7 cells in 8 out of 10 breast cancer patients (80 %), with cell counts ranging from 2 to 21 cells/mL and purities between 89 ± 1.6 % and 92 ± 5.8 %. These results confirm the chip’s high sensitivity and viability preservation. This straightforward inertial microfluidic method offers a promising alternative to traditional affinity-based CTC separation, supporting fundamental research and aiding patient treatment strategies.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109788"},"PeriodicalIF":3.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shifts in microbial communities and antibiotic resistance genes in activated sludge driven by varying heterotrophic nitrifying aerobic denitrifying bacteria ratios 不同异养硝化好氧反硝化细菌比例驱动的活性污泥中微生物群落和抗生素抗性基因的变化

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-16 DOI: 10.1016/j.bej.2025.109790

Xiao-Yan Fan , Ji-Gang Ma , Wen-Nian Geng , Jia-Wei Ren , Shen-Bin Cao , Qing-Liang Sun , Jun-Ru Zhao

{"title":"Shifts in microbial communities and antibiotic resistance genes in activated sludge driven by varying heterotrophic nitrifying aerobic denitrifying bacteria ratios","authors":"Xiao-Yan Fan , Ji-Gang Ma , Wen-Nian Geng , Jia-Wei Ren , Shen-Bin Cao , Qing-Liang Sun , Jun-Ru Zhao","doi":"10.1016/j.bej.2025.109790","DOIUrl":"10.1016/j.bej.2025.109790","url":null,"abstract":"<div><div>Activated sludge systems experience reduced microbial activity under low-temperature conditions, which adversely affects the biological nitrogen removal process. Heterotrophic nitrifying aerobic denitrifying bacteria (HNADB), with their strong tolerance to low temperatures, present a promising solution through bioaugmentation. This study explored the bio-enhancement of activated sludge systems using HNADB to improve nitrogen removal, and evaluated microbial community structure, functional genes, and antibiotic resistance genes (ARGs) through batch experiments with varying inoculation ratios (from 0 % to 100 %) of HNADB-enriched sludge. Higher inoculation ratios (80 % and 100 %) significantly enhanced total nitrogen (TN) removal by 32.75 % and 43.62 %, respectively, though nitrification efficiency declined. Community diversity increased, while richness decreased under higher inoculation. Abundant taxa (AT) constituted the dominant proportion in all systems and were further enriched in the high-inoculation treatments. High inoculation ratios enriched denitrifying bacteria (DNB), HNADB (<em>Thauera</em>, <em>Hyphomicrobium</em>, <em>Rhodococcus</em>), and their related functional genes, while decreasing the abundance of nitrifying microorganisms and genes. The ARGs diversity increased but abundance decreased over time. DNB and HNADB may serve as hosts for ARGs, while nitrifying microorganisms show higher sensitivity to antibiotics. This study highlighted the potential of HNADB-based inoculation to enhance nitrogen removal performance and community resilience under low-temperature conditions, providing an effective biological enhancement strategy for low-temperature wastewater treatment.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109790"},"PeriodicalIF":3.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecularly-imprinted polymer based on graphene oxide functionalized pencil graphite electrode for cholesterol detection 基于氧化石墨烯功能化铅笔石墨电极的分子印迹聚合物用于胆固醇检测

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-16 DOI: 10.1016/j.bej.2025.109769

Fera Ayu Dianovita , Elly Septia Yulianti , Siti Hanafiah , Siti Fauziyah Rahman

{"title":"Molecularly-imprinted polymer based on graphene oxide functionalized pencil graphite electrode for cholesterol detection","authors":"Fera Ayu Dianovita , Elly Septia Yulianti , Siti Hanafiah , Siti Fauziyah Rahman","doi":"10.1016/j.bej.2025.109769","DOIUrl":"10.1016/j.bej.2025.109769","url":null,"abstract":"<div><div>Cholesterol plays a vital role in biological functions that must be maintained at a normal level of < 5.2 mmol/L to prevent hypercholesterolemia, leading to cardiovascular diseases. This research examines a molecularly-imprinted polymer (MIP) based biosensor using a pencil graphite electrode (PGE) modified graphene oxide (GO) for the sensitive, selective, and stable detection of cholesterol with a simple approach using the electropolymerization process. The imprinted poly[2-(dimethylamino) ethyl methacrylate] (poly[DMAEMA]) layers on PGE/GO surface exhibit strong electrochemical responses during cholesterol detection through the formation of cholesterol-specific recognition cavities that were formed to mimic the cholesterol’s structures, allowing non-enzymatic detection. Through the optimization of the parameters during electropolymerization, including the ratio of template molecule concentration to monomer, polymerization and template removal cycles, scan rate, rebinding duration, and pH, the sensor demonstrated satisfactory performance. With the limit of detection (LOD) of 0.85 mM, limit of quantification (LOQ) of 2.85 mM, a linear range of 1–6 mM, and sensitivity of 40.52 μA.μM⁻¹ .cm⁻², the sensor offered good electrochemical performance and selectivity towards cholesterol, despite the presence of other interference molecules. The current stability percentage on the tenth day was 85.52 %, which indicates good stability of the sensor modification and indicates potential for repeated cholesterol sensing for further point-of-care testing (POCT) application.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109769"},"PeriodicalIF":3.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144106669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal dynamics of algal-microbial interactions in a membrane photobioreactor for integrated nutrient remediation and microalgal resource recovery from shrimp aquaculture wastewater 膜光生物反应器中藻类与微生物相互作用的时空动态研究——用于对虾养殖废水营养物综合修复和微藻资源回收

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-16 DOI: 10.1016/j.bej.2025.109793

Tianyi Ye , Miaoxin Li , Derek M. Ayittey , Yuanyuan Qi , Zheng Sun , Zhijuan Jiang , Guoqi Zhang , Yun Ling

{"title":"Spatiotemporal dynamics of algal-microbial interactions in a membrane photobioreactor for integrated nutrient remediation and microalgal resource recovery from shrimp aquaculture wastewater","authors":"Tianyi Ye , Miaoxin Li , Derek M. Ayittey , Yuanyuan Qi , Zheng Sun , Zhijuan Jiang , Guoqi Zhang , Yun Ling","doi":"10.1016/j.bej.2025.109793","DOIUrl":"10.1016/j.bej.2025.109793","url":null,"abstract":"<div><div>The projected rise of global aquaculture to 106 million tons by 2030 has amplified concerns over nutrient-rich wastewater, demanding sustainable treatment solutions. This study developed an aerated membrane photobioreactor (MPBR) to simultaneously assess nutrient removal, biomass productivity, and algal–microbial community dynamics. High-throughput sequencing identified a dominant, stable consortium featuring the nitrogen-fixing <em>Azospirillum</em> (20.81 %) and the eukaryotic alga <em>Halochlorella</em> (53.19 %). Membrane surface analysis revealed a diverse biofilm community including <em>Limnothrix</em> (12.36 %) and <em>Scenedesmus</em> (28.70 %), suggesting synergistic microbial interactions. Isolated dominant species achieved total nitrogen (TN) removal of 58.36 %–65.50 %, total phosphate (TP) removal of 97.68 %–100 %, and ammonium (NH₄⁺-N) removal of 97.37 %–100 % at a 10-hour hydraulic retention time. These findings underscore the MPBR's dual functionality in effective nutrient remediation and microalgal biomass valorization, offering an informed strategy for resource recovery and environmentally sustainable aquaculture wastewater management.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109793"},"PeriodicalIF":3.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144154903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0