Biochemical Engineering Journal最新文献_第3页

Recombinant beta-galactosidase derived from Enterobacter cloacae Zjut HJ2001 for efficient biotransformation of galactooligosaccharides 用于高效生物转化半乳寡糖的重组β-半乳糖苷酶，来源于泄殖腔肠杆菌 Zjut HJ2001

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-09-25 DOI: 10.1016/j.bej.2024.109514

{"title":"Recombinant beta-galactosidase derived from Enterobacter cloacae Zjut HJ2001 for efficient biotransformation of galactooligosaccharides","authors":"","doi":"10.1016/j.bej.2024.109514","DOIUrl":"10.1016/j.bej.2024.109514","url":null,"abstract":"<div><div>Galactooligosaccharides (GOS), as a type of prebiotics, have excellent physical and chemical properties, and can be widely used in the pharmaceutical and food fields. Recently, the microbial β-galactosidases have gained widespread attentions in industrial GOS production. However, most β-galactosidases from microorganisms have low transgalactosylation activity, resulting in poor GOS yield of enzymatic transformation from lactose. In this paper, a brand new β-galactosidase derived from <em>Enterobacter cloaca</em>e Zjut HJ2001 was screened out from soil, and successfully overexpressed, characterized, and mutated by combinatorial alanine-scanning and site-saturation mutagenesis. Compared to the yield of 51.73 % obtained by wild-type β-galactosidase with lactose concentration of 380 g/L, the obtained mutant β-gal-H542V achieved a higher GOS yield of 67.08 %, which was the highest in the reported literature. These results suggested that the developed mutagenesis strategy could improve the transgalactosylation efficiency, and the mutant β-gal-H542V could be regarded as a prospective biocatalyst for GOS industrial manufacturing.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Copper and zinc sulfides bioleaching by an extremely thermophilic archaeon in high NaCl concentration 高浓度氯化钠条件下极嗜热古生物对硫化铜和硫化锌的生物浸出作用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-09-24 DOI: 10.1016/j.bej.2024.109509

{"title":"Copper and zinc sulfides bioleaching by an extremely thermophilic archaeon in high NaCl concentration","authors":"","doi":"10.1016/j.bej.2024.109509","DOIUrl":"10.1016/j.bej.2024.109509","url":null,"abstract":"<div><div>Chloride bioleaching has received attention of several mineral processing industries, particularly in countries where there is scarcity of freshwater and only chloride-containing waters can be used. Therefore, the present work investigated the effect of NaCl (1.0 mol L<sup>−1</sup>) on the bioleaching of three sulfide minerals: chalcopyrite, bornite, and sphalerite by the thermophilic archaea <em>Sulfolobus acidocaldarius</em>. Chalcopyrite dissolution was only 25 % in the biotic experiment in the absence of chloride, but reached 90 % in the presence of both microorganisms and chloride, while less than 60 % extraction was observed in the abiotic experiment with chloride. In the experiments of bornite bioleaching, 86 % and 77 % of copper were extracted in the biotic and abiotic tests with chloride, respectively. In the absence of NaCl, the biotic and abiotic experiments presented similar copper dissolution (∼35 %). Finally, bioleaching experiments carried out with sphalerite showed zinc extractions below 35 % in all conditions tested. The main contribution from the archaea was its ability to produce low concentrations of ferric ion, which was partially precipitated as jarosite, resulting in low redox potential values (< 450 mV vs. Ag/AgCl), and efficiently bioleached bornite and chalcopyrite. Furthermore, XRD and SEM-EDS analyses demonstrated that sphalerite was practically not leached while bornite was transformed into new copper sulfide phases (CuS and Cu<sub>3</sub>FeS<sub>4</sub>). Jarosite and elemental sulfur were products of chalcopyrite and bornite bioleaching in the presence of chloride.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142327203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of in situ product recovery techniques to enhance 2-phenylethanol production by Acinetobacter soli ANG344B 评估原位产品回收技术，以提高固氮醋酸杆菌 ANG344B 的 2-苯基乙醇产量

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-09-23 DOI: 10.1016/j.bej.2024.109508

{"title":"Assessment of in situ product recovery techniques to enhance 2-phenylethanol production by Acinetobacter soli ANG344B","authors":"","doi":"10.1016/j.bej.2024.109508","DOIUrl":"10.1016/j.bej.2024.109508","url":null,"abstract":"<div><div>The 2-phenylethanol (2-PE) production process by the newly isolated <em>Acinetobacter soli</em> ANG344B is limited by product toxicity. To overcome this limitation and enhance 2-PE production process, various alternatives based in <em>in situ</em> product removal (ISPR) approaches were evaluated. The approaches selected for assessment were gas stripping using the air supplied to the bioreactor, liquid-liquid extraction and adsorption. Adsorption was found to be the most promising approach to increase 2-PE production. Amberlite XAD 4 was chosen from the different adsorbents tested since it has high affinity for 2-PE, being able to adsorb 205.8 ± 8.1 mg<sub>2-PE</sub>/g<sub>dry resin</sub>. In a batch cultivation process, in presence of 3 % (dry w/v) of Amberlite XAD 4, <em>A. soli</em> ANG344B was able to produce 6.99 ± 0.06 g/L of 2-PE with a volumetric productivity of 0.17 ± 0.00 g/L.h, which represents an improvement of 3.3-fold. To the best of our knowledge, this is the highest 2-PE production reported for a wild-type bacteria. These findings highlight the potential of <em>Acinetobacter soli</em> ANG344B as 2-PE producer, contributing to the development of natural 2-PE production process.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142327202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-free expression and biochemical characterization of polysaccharide-synthesizing glycosyltransferases 多糖合成糖基转移酶的无细胞表达和生化鉴定

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-09-23 DOI: 10.1016/j.bej.2024.109507

{"title":"Cell-free expression and biochemical characterization of polysaccharide-synthesizing glycosyltransferases","authors":"","doi":"10.1016/j.bej.2024.109507","DOIUrl":"10.1016/j.bej.2024.109507","url":null,"abstract":"<div><div>Polysaccharides like cellulose and hyaluronan are synthesized by membrane-bound family-2 glycosyltransferases (GTs) that play critical structural, metabolic, or functional roles in cells. Though GT-2 family has the maximum number of deposited gene sequences, the biochemistry is poorly understood due to enzyme production challenges. Here, we developed a cell-free expression (CFE) protocol to produce two GT-2 family representative cellulose synthase (PttCesA8 from <em>Populus tremula x tremuloides</em>) and hyaluronan synthase (SeHAS from <em>Streptococcus equisimilis</em>). The CFE products were obtained as reconstituted proteoliposomes directly at high yields and short processing times compared to other approaches. Enzyme expression was confirmed using SDS-PAGE and immunoblotting, while integration of GTs into lipid layers was observed using cryogenic electron microscopy. Both GTs were catalytically active with Michalis-Menten kinetic constants, K<sub>m</sub> for PttCesA8, was 295.8 µM, and SeHAS was 321.51 µM (toward UDP N-Acetyl Glucosamine) and 207.88 µM (toward UDP Glucuronic Acid), respectively, and with UDP inhibiting both GTs. Mutation of conserved residues in SeHAS also confirmed the importance of lysine-139, glutamine-248, and threonine-283 residues in hyaluronan biosynthesis. In summary, CFE methods enable expression of polysaccharide-synthesizing enzymes as proteoliposomes at high yields with relative ease for rapid biochemical and structural characterization studies.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142320375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction of poly(3-hydroxybutirate) from Priestia megaterium using non-halogenated solvents: A comparative performance analysis 使用非卤化溶剂从巨栉藻中提取聚（3-羟基丁酸酯）：性能比较分析

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-09-21 DOI: 10.1016/j.bej.2024.109502

{"title":"Extraction of poly(3-hydroxybutirate) from Priestia megaterium using non-halogenated solvents: A comparative performance analysis","authors":"","doi":"10.1016/j.bej.2024.109502","DOIUrl":"10.1016/j.bej.2024.109502","url":null,"abstract":"<div><div>Solvent extraction using chloroform is the most common industrial process for poly(3-hydroxybutyrate) (P(3HB)) recovery from dried biomass, and still a major barrier to expanding the commercial application of this biodegradable biopolymer. Consequently, there is great interest in alternative non-halogenated solvents for this process and some relevant related results are available in the literature for P(3HB) recovery from Gram-negative bacteria. This work evaluated the potential of a set of non-halogenated solvents for the extraction of P(3HB) from <em>Priestia megaterium</em>, a Gram-positive bacterium of great potential for P(3HB) production. Ethyl acetate (EtAc), methyl ethyl ketone (MEK), dimethyl carbonate (DMC), dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (NNDA), 2-heptanone (2-Hp), propylene carbonate (PC), and isoamyl propionate (IAP) were tested. Preliminary solubilization tests using commercial P(3HB) showed that EtAc, MEK, DMC and IAP had lower P(3HB) solubilization capacity (below 0.08 g/L for EtAc, MEK, and DMC; 1.3–2.5 g/L for IAP) than DMSO (65–70 g/L) and PC, 2-Hp and NNDA (>100 g/L). Then, only DMSO, PC, 2-Hp, and NNDA were evaluated in recovery tests with intracellular P(3HB). DMSO was not selective for P(3HB), causing digestion of cell wall components. PC, 2-Hp, and NNDA outperformed chloroform, but NNDA stood out for its remarkably higher recovery (98.5 %, 30 min, 140 ºC).</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142323941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of phenyllactic acid biosynthesis and separation by machine learning with neural network and overlay sampling uniform design 利用神经网络和叠加采样统一设计的机器学习优化苯乳酸的生物合成和分离

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-09-21 DOI: 10.1016/j.bej.2024.109506

{"title":"Optimization of phenyllactic acid biosynthesis and separation by machine learning with neural network and overlay sampling uniform design","authors":"","doi":"10.1016/j.bej.2024.109506","DOIUrl":"10.1016/j.bej.2024.109506","url":null,"abstract":"<div><div>Machine learning methodology with neural network models was developed using the datasets based on the overlay sampling uniform design (OSUD) for optimization of phenyllactic acid biosynthesis and separation processes by cryogels. Compared with the multiple regression, the machine learning models exhibited a significant improvement of predictive accuracy of phenyllactic acid biosynthesis, in which the radial basis function neural network (RBFNN) model had the best predictive performance with the accuracy increased by 65.2 %. The combination of RBFNN and OSUD was further employed to optimize the chromatographic separation of phenyllactic acid from crude fermentation broth using two poly(hydroxyethyl methacrylate) based anion-exchange cryogel packed-beds (grafted with (vinylbenzyl)trimethylammonium chloride and <em>N,N</em>-dimethylaminoethyl methacrylate). After optimizing the three critical separation parameters: sample volume (5.3–31.8 mL), flow velocity (1.0–6.0 cm/min), and elution salt concentration (0.05–0.3 mol/L), it was found that the models provided excellent predictions. The optimized recovery rates for the two packed-beds were determined to be 76.5 % and 83.0 %, and the optimal adsorption capacities were 0.26 mg/mL and 0.39 mg/mL from the fermentation broth, respectively. This study provides a reliable integrated approach for optimizing the synthesis and separation processes of high-value bioproducts like phenyllactic acid from crude feedstocks.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142320374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response of the performance and succession of denitrification consortium under the variation of nutritional conditions: Mechanisms and characteristics 营养条件变化对反硝化联合体性能和演替的影响：机理与特征

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-09-20 DOI: 10.1016/j.bej.2024.109503

{"title":"Response of the performance and succession of denitrification consortium under the variation of nutritional conditions: Mechanisms and characteristics","authors":"","doi":"10.1016/j.bej.2024.109503","DOIUrl":"10.1016/j.bej.2024.109503","url":null,"abstract":"<div><div>In the process of wastewater treatment, biomass was typically subjected to variation of nutrient condition. This study investigated the effects of different nutrient condition on denitrification performance and bacterial communities. The results showed that under carbon-to-nitrogen ratio (C/N) was 4, denitrifying sludge (DS) exhibited excellent denitrification performance with influent nitrate (NO<sub>3</sub><sup>-</sup>-N) concentration in the range of 88.8 ± 9.04–297.5 ± 6.63 mg L<sup>−1</sup>, maintained total nitrogen removal efficiency (TNRE) of 98.1 ± 1.75 %. While, as influent NO<sub>3</sub><sup>-</sup>-N concentration reached 408.9 ± 9.56 mg L<sup>−1</sup>, the denitrification performance was inhibited, and this inhibition was reversible. Under appropriate substrate level (215.0 ± 13.01 mg L<sup>−1</sup>), the specific denitrification activity (SDA) elevated to 300 % of its original value. Additionally, under starvation stress, although the relative abundance of some starvation-resistant bacteria (such as <em>Actinobacteriota</em> and <em>Lentimicrobium</em>) increased, the TNRE decreased by 7.3 %. During the recovery phase, despite extracellular polymeric substances (EPS) promoted, the TNRE decreased to 78.3 ± 4.7 %. Starvation stress was less favorable to DS for activity recovery than that of feast condition. These findings contribute to elucidating the mechanisms for DS to respond of different nutrient condition.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Economic validation and comparison of microbial tryptophan, erythritol and collagen production in an integrated sugarcane biorefinery 综合甘蔗生物精炼厂中微生物色氨酸、赤藓糖醇和胶原蛋白生产的经济验证与比较

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-09-19 DOI: 10.1016/j.bej.2024.109505

{"title":"Economic validation and comparison of microbial tryptophan, erythritol and collagen production in an integrated sugarcane biorefinery","authors":"","doi":"10.1016/j.bej.2024.109505","DOIUrl":"10.1016/j.bej.2024.109505","url":null,"abstract":"<div><p>Low sugar prices present a significant challenge to the global sugarcane industry, prompting the exploration of diversification strategies for expanding product portfolios. Techno-economic analyses and environmental sustainability assessments were carried out to evaluate the microbial production of tryptophan, erythritol, and collagen from A-molasses in a biorefinery annexed to an existing sugarcane mill. Tryptophan production exhibited the highest profitability, with a minimum selling price (MSP) at 59.7 % of its current market price, although the achievable production volumes of tryptophan from one sugar mill would oversupply the global market. Due to the larger market size of for collagen the achievable production capacity in the collagen scenario would avoid market saturation, reducing the risk of oversupply and rendering it more economically viable. In contrast, erythritol production was marginally not profitable, with an MSP exceeding the current market price by 1 %, primarily attributed to high operational costs. All scenarios demonstrated relatively low greenhouse gas (GHG) emissions (ranging from 9.1 to 16.5 kg CO2eq/kg product), with tryptophan production emerging as the most environmentally favourable option due to minimal chemical and freshwater usage. When compared with literature-reported data on ethanol and short-chain fructooligosaccharides (scFOS), only collagen and ethanol production were deemed viable, based on their favourable profitability and contribution to the market.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1369703X24002924/pdfft?md5=ebbb808db23b0919218e353be42c77f8&pid=1-s2.0-S1369703X24002924-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142274150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation on potential application of lipase immobilized on rice husks in enzymatic glycerolysis reaction 评估固定在稻壳上的脂肪酶在酶解甘油反应中的应用潜力

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-09-17 DOI: 10.1016/j.bej.2024.109500

{"title":"Evaluation on potential application of lipase immobilized on rice husks in enzymatic glycerolysis reaction","authors":"","doi":"10.1016/j.bej.2024.109500","DOIUrl":"10.1016/j.bej.2024.109500","url":null,"abstract":"<div><p>Owing to high production cost and low reaction yield, immobilized lipase is rarely used in industrial glycerolysis. This research characterizes the performance of lipase immobilized on rice husk in glycerolysis reaction. By utilizing hexamethylenediamine (HMDA) and glutaraldehyde (GA) as coupling agents, lipase from <em>Thermomyces lanuginosus</em> was immobilized on oxidized rice husk (ORH). For comparison, another sample was prepared where the lipase was directly immobilized on ORH without the use of HMDA and GA. Then, monoglyceride production was performed via glycerolysis using the immobilized lipase. The FTIR analysis verify interactions on rice husk including rice husk oxidation, HMDA coupling, GA activation and lipase immobilization on rice husk. The study found that within the examined range of added lipase for immobilization (10–40 mg-protein/g-support), ORH–HMDA–GA–Lipase possessed superior outcomes in terms of protein loading, immobilization yield, and recovered glycerolysis activity compared to ORH–Lipase. Besides, ORH–HMDA–GA–Lipase exhibits better storage stability (60°C, 44.9 %) and higher reusability (90.0 % monoglyceride yield at the 8th cycle) against ORH–Lipase. The results confirm satisfying performance of the prepared immobilized lipase in glycerolysis and highlight its enhancements facilitated by coupling agents.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142274152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The solidification of heavy metal Pb2+-contaminated soil by enzyme-induced calcium carbonate precipitation combined with biochar 通过酶诱导碳酸钙沉淀结合生物炭固化重金属 Pb2+ 污染的土壤

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-09-17 DOI: 10.1016/j.bej.2024.109496

引用次数: 0