Biochemical Engineering Journal最新文献_第4页

Au@CeO2 nanozyme based smart colourimetric sensor for cholesterol: A neural network powered point of care solution model Au@CeO2基于纳米酶的胆固醇智能比色传感器：神经网络驱动的护理点解决方案模型

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-08-26 DOI: 10.1016/j.bej.2025.109908

Poornima Govindharaj , Nihad Alungal , Caxton Emerald. S , Kannan. S

{"title":"Au@CeO2 nanozyme based smart colourimetric sensor for cholesterol: A neural network powered point of care solution model","authors":"Poornima Govindharaj , Nihad Alungal , Caxton Emerald. S , Kannan. S","doi":"10.1016/j.bej.2025.109908","DOIUrl":"10.1016/j.bej.2025.109908","url":null,"abstract":"<div><div>The current investigation aims to provide a ready-to-use biosensor with the aid of an ANN-powered point-of-care solution model for the detection of cholesterol. The synthesized AuNP@CeO<sub>2</sub> nanoparticles exhibited strong catalytic performance with the respective K<sub>m</sub> (Michaelis-Menten constant) and V<sub>max</sub> (maximum reaction velocity) values of 5.3255 mM and 0.00406 mM s⁻¹ of cholesterol. Both the visual observation and UV-Vis spectroscopy verified the colourimetric shift from blue-green to light blue. The image J results established a good linearity in the cholesterol content ranging from 0 to 50 mM, with a regression equation of y = 128.40 + 0.653x and R<sup>2</sup> value of 0.9842. Similarly, the optimal validation mean squared error (MSE) of 0.0577 has been achieved using the hard shrink activation with 13 neurons. The lowest training MSE value of 0.0637 is observed with the SELU activation function at 7 neurons. The median training and validated MSEs across all models are in the order of 0.0896 and 0.0769. Activation functions such as hard shrink, Selu, Hard Tanh, Relu6 and Tanh exhibited a uniform and consistent performance. The overall results support the effectiveness of nanozymes and ANNs for the biomedical regression tasks, especially in small sample scenarios where the capture of non-linear interactions is critical.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"225 ","pages":"Article 109908"},"PeriodicalIF":3.7,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of ultrasonic sludge reflux in an improved anaerobic/anoxic/oxic(A2/O) system for municipal wastewater treatment plant: Pollutant removal, sludge reduction, and microbial community 超声污泥回流对城市污水处理厂厌氧/缺氧/好氧（A2/O）系统的影响：污染物去除、污泥减少和微生物群落

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-08-25 DOI: 10.1016/j.bej.2025.109910

Jin-Ming Qiu , Hong-Yu Jiang , Yi-Juan Yan , Chen-Shun Lu , Xiang Li , Takuro Kobayashi , Yong Hu

{"title":"Effects of ultrasonic sludge reflux in an improved anaerobic/anoxic/oxic(A2/O) system for municipal wastewater treatment plant: Pollutant removal, sludge reduction, and microbial community","authors":"Jin-Ming Qiu , Hong-Yu Jiang , Yi-Juan Yan , Chen-Shun Lu , Xiang Li , Takuro Kobayashi , Yong Hu","doi":"10.1016/j.bej.2025.109910","DOIUrl":"10.1016/j.bej.2025.109910","url":null,"abstract":"<div><div>Municipal wastewater treatment plants (MWWTPs) are currently facing challenges like low carbon sources of influent and excess sludge. Utilizing ultrasonic sludge disintegration can help supplement the carbon source for the biochemical tank and achieve in-situ sludge reduction. A full-scale experiment was conducted at an MWWTP to investigate the impact of reflux points on the performance of an improved A<sup>2</sup>/O system, and the reflux points included pre-anoxic (PrAX), anterior anoxic (AAX) and anaerobic (AN) tanks. Results showed that the use of ultrasound did not impact the effluent quality of the improved A<sup>2</sup>/O system. The optimal reflux point was AN, resulting in a 37.4 % sludge reduction while enhancing sludge settleability (SVI decreased by 13.39 mL/g). Nitrogen balance calculations revealed that refluxing disintegrated sludge to AAX increased its denitrification and nitrogen removal efficiency (DNR) by 12.22 %. The supplementation of organic matter also increased the relative abundance of <em>Acidobacteriota</em> (3.19 %) in the AAX. Refluxing to AN increased the denitrification phosphorus removal efficiency (DPR) of AAX by 32.02 %. AAX fully utilized the more storable carbon sources provided by ultrasonic sludge for AN. Refluxing to AN increased the relative abundance of <em>Bacteroidota</em> in AN by 5.47 %. This study investigated the synergistic effects of different sludge reflux points on pollutant removal and sludge reduction, providing a practical basis for implementing sludge ultrasonic treatment in MWWTPs.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"225 ","pages":"Article 109910"},"PeriodicalIF":3.7,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing CRISPR homology directed repair in IAL-PiD2 insect cells via reagent delivery optimization and cell synchronization 通过试剂递送优化和细胞同步增强昆虫il - pid2细胞的CRISPR同源性定向修复

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-08-21 DOI: 10.1016/j.bej.2025.109906

Bryce D. Shirk , Cecilia Z. Rodriguez , Marisa O. Pacheco , Jasmine B. McTyer , Paul D. Shirk , Whitney L. Stoppel

{"title":"Enhancing CRISPR homology directed repair in IAL-PiD2 insect cells via reagent delivery optimization and cell synchronization","authors":"Bryce D. Shirk , Cecilia Z. Rodriguez , Marisa O. Pacheco , Jasmine B. McTyer , Paul D. Shirk , Whitney L. Stoppel","doi":"10.1016/j.bej.2025.109906","DOIUrl":"10.1016/j.bej.2025.109906","url":null,"abstract":"<div><div>CRISPR/Cas9-mediated homology-directed repair (HDR) enables precise genome editing, yet its application in insect cell lines remains largely unexplored. Insect systems, already used in recombinant protein production via baculovirus expression, offer substantial potential for stable, non-viral genetic modification using HDR-based approaches. This study establishes a robust, reproducible HDR framework in <em>Plodia interpunctella</em> IAL-PiD2 cells, a lepidopteran species increasingly relevant in pest management and biomaterials research. We systematically evaluated factors influencing HDR efficiency, including transfection reagent, Cas9:sgRNA molar ratios, donor DNA concentration, homology arm length, and cell cycle synchronization. A ribonucleoprotein molar ratio of 1:1 and 0.66 pmol donor DNA template (∼3.97 × 10 ¹¹ copies) yielded the highest integration efficiency under standard transfection conditions. Cell cycle synchronization using hydroxyurea followed by transfection 4 h post-treatment produced a 1.57-fold increase in HDR efficiency versus asynchronous controls. This is the first demonstration of regulated cell cycle timing improving HDR in insect cells, emphasizing the importance of delivery timing for efficient editing. These findings establish cost-effective, scalable protocols for non-viral gene delivery in insect cells and position IAL-PiD2 as a viable platform for functional genomics, precision pest control, and recombinant protein production. The results also support <em>in vivo</em> applications of HDR, enabling precise genetic modifications for a broad range of investigative and applied purposes.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"225 ","pages":"Article 109906"},"PeriodicalIF":3.7,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144895623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Triple-functional biohybrid for efficient Cr (VI) remediation: Synergistic adsorption, chemoreduction and bioreduction 高效修复Cr （VI）的三功能生物杂化：协同吸附、化学还原和生物还原

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-08-20 DOI: 10.1016/j.bej.2025.109907

Xinyu Li, Mingzhao Liang, Zhihong Zhang, Jianfeng Zhang, Jing Liang

{"title":"Triple-functional biohybrid for efficient Cr (VI) remediation: Synergistic adsorption, chemoreduction and bioreduction","authors":"Xinyu Li, Mingzhao Liang, Zhihong Zhang, Jianfeng Zhang, Jing Liang","doi":"10.1016/j.bej.2025.109907","DOIUrl":"10.1016/j.bej.2025.109907","url":null,"abstract":"<div><div>The reduction and adsorption of Cr(VI) are critical challenges in environmental remediation. In this study, corn straw modified with polyethyleneimine (PEI-CS) was synthesized as carriers to immobilize <em>Bacillus licheniformis</em> MZ1 (PEI-CS-MZ1), enabling simultaneous Cr(VI) reduction and Cr(III) fixation in a high efficiency. Notably, PEI-CS-MZ1 achieved 95.61 % and 81.96 % Cr(VI) reduction under extreme conditions (pH 8 and 11 % salinity), respectively. Furthermore, this composite completely reduced 100 mg/L Cr(VI) within 24 h while maintaining 76.96 % Cr(III) fixation, which was significantly superior to free MZ1 and PEI-CS. PEI-CS-MZ1 also demonstrated exceptional stability, retaining 89.8 % Cr(VI) reduction after 10 operational cycles. This strategy revealed that Cr(VI) reduction and Cr(III) fixation were accomplished <em>via</em> cooperativity of bio-reduction, chemical reduction, chemisorption, and physical adsorption. This work provides theoretical foundations for designing immobilized microbial systems toward efficient remediation of Cr(VI)-contaminated wastewater.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"224 ","pages":"Article 109907"},"PeriodicalIF":3.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of bioactive channels for air treatment with COVID-19 by numerical simulations 通过数值模拟开发用于COVID-19空气处理的生物活性通道

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-08-19 DOI: 10.1016/j.bej.2025.109905

Bruna Iten Bittelbrunn , Harrson Silva Santana , João Lameu da Silva Junior , Dirceu Noriler , Osvaldir Pereira Taranto

{"title":"Development of bioactive channels for air treatment with COVID-19 by numerical simulations","authors":"Bruna Iten Bittelbrunn , Harrson Silva Santana , João Lameu da Silva Junior , Dirceu Noriler , Osvaldir Pereira Taranto","doi":"10.1016/j.bej.2025.109905","DOIUrl":"10.1016/j.bej.2025.109905","url":null,"abstract":"<div><div>COVID-19 is a severe respiratory disease caused by SARS-CoV-2 coronavirus which was declared a pandemic in March 2020. It is a highly contagious disease transmitted through air by microscopic aerosols as infected people breath, sneeze, cough, and speak. Airborne transmission can also occur in confined places, especially in the immediate environment of the infected person. Therefore, environments such as hospital rooms need to be constantly disinfected and this includes exhaust air. In this context, the present study numerically investigates the efficiency of microfluidic devices with heterogeneous active surfaces to adsorb and deactivate target species (e.g. virus) from ICU hospital rooms exhaust air. A verified and validated numerical model was applied to investigate different device configurations by altering channel height, inlet velocity, and inlet concentrations. To analyze target species deactivation, eleven designs were studied. The best performance was achieved by G10 design (H 1.2 x W 10 x L 50 mm) with active parallel channels at top and bottom surfaces. A 45.8 % deactivation was achieved (260 times greater than the original biosensor) with an 11 times larger flow rate.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"224 ","pages":"Article 109905"},"PeriodicalIF":3.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144866907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From macrostructure to machine learning: Lava rock as a superior carrier in anaerobic co-digestion of manure and molasses residue 从宏观结构到机器学习：熔岩作为粪便和糖蜜残渣厌氧共消化的优越载体

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-08-18 DOI: 10.1016/j.bej.2025.109904

Khairina Jaman , Syazwani Idrus , Razif Harun , Nik Norsyahariati Nik Daud , Balqis Mohamed Rehan , Amimul Ahsan , Ain Fitriah Zamrisham

{"title":"From macrostructure to machine learning: Lava rock as a superior carrier in anaerobic co-digestion of manure and molasses residue","authors":"Khairina Jaman , Syazwani Idrus , Razif Harun , Nik Norsyahariati Nik Daud , Balqis Mohamed Rehan , Amimul Ahsan , Ain Fitriah Zamrisham","doi":"10.1016/j.bej.2025.109904","DOIUrl":"10.1016/j.bej.2025.109904","url":null,"abstract":"<div><div>This study investigates the anaerobic co-digestion of cow manure (CM) and molasses residue (MR), focusing on the impact of various support carriers on reactor performance and machine learning model predictions. BMP tests identified a 50:50 CM:MR ratio as optimal for methane production, yielding the highest biogas production (1540 mL), SMP (45.05 mLCH₄/gVS<sub>added</sub>), and VS removal (51.4 %). Semi-continuous experiments were conducted with support carriers—lava rock (LR), nanoparticles (NPs), biochar (BC), and synthetic grass (SG), under mesophilic conditions with the 50:50 CM:MR ratio and organic loading rates of 1–6 gVS/L/day for 100 days. LR showed the best performance, producing the highest biogas (170 mL), SMP (22.5 mL CH₄/gVS<sub>added</sub>), and VS removal (59.8 %). Compared to other support carriers, LR exhibited the largest pore size at 53.7 nm (92 % larger than BC and 88.6 % larger than NPs), which significantly enhanced nutrient diffusion and microbial accessibility. Machine learning models, including ANN and SVM, were developed from BMP data, with SVM showing superior predictive accuracy (R² = 0.84373) compared to ANN (R² = 0.71367). SEM and EPS analyses revealed a higher microbial population on LR than on BC. These results suggest LR’s large pore size make it a promising support carrier for improving AD performance.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"224 ","pages":"Article 109904"},"PeriodicalIF":3.7,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144866906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in the biosynthesis of heparosan, a key precursor of heparin 肝素关键前体肝素聚糖的生物合成研究进展

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-08-15 DOI: 10.1016/j.bej.2025.109903

Haoxian Chen, Haoyu Dong, Ruoxue Lu, Xingyuan Wang, Zheng-Jun Li

{"title":"Advances in the biosynthesis of heparosan, a key precursor of heparin","authors":"Haoxian Chen, Haoyu Dong, Ruoxue Lu, Xingyuan Wang, Zheng-Jun Li","doi":"10.1016/j.bej.2025.109903","DOIUrl":"10.1016/j.bej.2025.109903","url":null,"abstract":"<div><div>Heparin, a vital anticoagulant widely used in clinical practice, has traditionally been sourced through extraction from animal tissues. However, this production method faces significant challenges, including limited source availability, batch-to-batch variability, and potential safety risks associated with animal-derived products. These inherent limitations have prompted an urgent need for developing more efficient and consistent heparin production strategies. Recent advances in biotechnology have identified heparosan, a bacterial capsular polysaccharide, as a promising precursor for heparin production through targeted sulfation modifications. The application of metabolic engineering techniques has significantly enhanced the microbial synthesis of heparosan, paving the way for an innovative and environmentally friendly approach to heparin production. This emerging biotechnological method offers substantial advantages, including reduced production costs, increased yield consistency, and decreased dependence on animal-derived resources, while simultaneously addressing safety concerns associated with traditional methods. This comprehensive review systematically examines recent advancements in microbial heparosan synthesis, evaluates current challenges, and explores future directions in this rapidly evolving field.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"224 ","pages":"Article 109903"},"PeriodicalIF":3.7,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144852222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards sustainable biodiesel production: Modeling, simulation, and profit optimization of microalgae oil extraction 迈向可持续生物柴油生产：微藻油提取的建模、模拟和利润优化

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-08-14 DOI: 10.1016/j.bej.2025.109902

Heloísa Roberto P. da Silva , Iago G. Costa , Anne C.D. Oliveira , Ihana A. Severo , Juan C. Nanclares , Juan C. Ordonez , José V.C. Vargas , André B. Mariano

{"title":"Towards sustainable biodiesel production: Modeling, simulation, and profit optimization of microalgae oil extraction","authors":"Heloísa Roberto P. da Silva , Iago G. Costa , Anne C.D. Oliveira , Ihana A. Severo , Juan C. Nanclares , Juan C. Ordonez , José V.C. Vargas , André B. Mariano","doi":"10.1016/j.bej.2025.109902","DOIUrl":"10.1016/j.bej.2025.109902","url":null,"abstract":"<div><div>The transition to renewable and sustainable energy sources requires efficient and economically viable biofuel production processes. This study investigated the extraction of lipids from the biomass of <em>Tetradesmus obliquus</em> microalgae cultivated in a 12 m<sup>3</sup> compact industrial photobioreactor. Extractions were performed using pure hexane, pure ethanol, and hexane-ethanol mixtures. The highest lipid extraction was produced by a solvent mixture of 30 % ethanol and 70 % hexane, yielding 3.25 g of lipid from 50 g of dry biomass. A mechanistic model incorporating mass transfer and solvent-lipid interactions reproduced the time-resolved data, yielding mean relative errors of 12 % for mixed solvents and 18 % for single solvents. The model identified the optimal solvent composition as a mixture containing 55 % ethanol. The preliminary economic analysis showed that, with an optimal solvent composition and working volume between 2000 and 4000 mL, the process yielded approximately $0.13 per gram of lipid extracted. These results demonstrate the potential for cost-effective, microalgae-based biodiesel production. The proposed modeling framework offers a valuable tool for optimizing extraction conditions and supporting the scaling up of processes.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"224 ","pages":"Article 109902"},"PeriodicalIF":3.7,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144858069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enrichment of microbial consortium and performance evaluation for single cell protein production in hydrogen-mediated microbial electrosynthesis reactors 氢介导微生物电合成反应器中微生物群落的富集及单细胞蛋白质生产性能评价

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-08-13 DOI: 10.1016/j.bej.2025.109901

Jinglei Tian , Xiaoying Shen , Yonggang Liu , Xueqi Wang , Jinzhe Liu , Yonglong Jin , Hong Zhang , Kai Cui , Kun Guo

{"title":"Enrichment of microbial consortium and performance evaluation for single cell protein production in hydrogen-mediated microbial electrosynthesis reactors","authors":"Jinglei Tian , Xiaoying Shen , Yonggang Liu , Xueqi Wang , Jinzhe Liu , Yonglong Jin , Hong Zhang , Kai Cui , Kun Guo","doi":"10.1016/j.bej.2025.109901","DOIUrl":"10.1016/j.bej.2025.109901","url":null,"abstract":"<div><div>The exponential expansion of the global population has raised substantial concerns regarding potential food security crises in the foreseeable future, compounded by environmental degradation and greenhouse gas emissions associated with conventional agricultural practices. Fortunately, edible microbial protein has emerged as a promising sustainable alternative. Thereinto, hydrogen-oxidizing bacteria (HOB) coupled with microbial electrosynthesis (MES) technology have garnered considerable attention for their potential in sustainable protein production. This investigation focused on enriching HOB compatible with electrochemical systems through H₂-mediated MES, utilizing ammonium as the nitrogen source. Following a 65-day cultivation period in a sleeve-type bioreactor, taxonomic analysis revealed that <em>Ancylobacter</em>, identified as the predominant HOB genus, constituted 86.89 % of the microbial community. Furthermore, through optimization of current operational parameters, we achieved a significant enhancement in biomass productivity of autotrophic HOB. The biomass yield reached 121 ± 1 mg/L/day, representing a 46 % increase compared to the initial enrichment rate. The protein content was quantified at 64 ± 2 %, demonstrating parity with bacterial and fish meal proteins, while substantially exceeding that of soybean meal.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"224 ","pages":"Article 109901"},"PeriodicalIF":3.7,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144852221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Addressing raw material variation: Maintaining a steady-state during cultivation by blending of lignocellulosic feed streams 解决原料变化：通过混合木质纤维素饲料流在培养过程中保持稳定状态

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-08-13 DOI: 10.1016/j.bej.2025.109891

Daniel Waldschitz , Johanna Bartlechner , Eva Maria Karner , Oliver Spadiut , Stefan Jakubek , Julian Kager

{"title":"Addressing raw material variation: Maintaining a steady-state during cultivation by blending of lignocellulosic feed streams","authors":"Daniel Waldschitz , Johanna Bartlechner , Eva Maria Karner , Oliver Spadiut , Stefan Jakubek , Julian Kager","doi":"10.1016/j.bej.2025.109891","DOIUrl":"10.1016/j.bej.2025.109891","url":null,"abstract":"<div><div>The transition to a sustainable bioeconomy necessitates the utilization of second-generation renewable resources. However, the inherent variability in the composition of these resources poses a challenge for establishing stable continuous bioprocesses. Industrial side-streams, such as spent sulfite liquor (SSL) from the pulp and paper industry, offer a promising starting point. Unlike first-generation processes, which rely on constant feed compositions, addressing the uncertainties of renewable resources requires robust monitoring and control strategies. To tackle the nonlinear dynamics of microbial conversion, we propose a model-based controller employing feedback linearization of a nonlinear model. Specifically, this study is focused on the uptake and bioconversion of sugars within SSL by <em>Corynebacterium glutamicum</em>. The controller dynamically adjusts mass flows into and out of the bioreactor to maintain steady-state conditions despite fluctuations in feed composition. Additionally, wood hydrolysate, rich in glucose and also from the paper and pulp industry, supplements the feed, enhancing control capabilities. Simulation studies showed that by actively controlling the process and adjusting the feed, harvest and bleed rates based on the sugar concentrations in the feed, process variability can be significantly reduced while sugar utilization increases. Experimentally, the introduced process was verified by continuous cultivation, whereby the process was effectively controlled and kept stable for more than 120 h with residual sugar concentrations always below 3.2 g/L. The decoupling of the multiple-input multiple-output system enables simultaneous and independent control of various process variables, establishing continuous cultivation processes resilient to raw material uncertainties and fluctuations.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"224 ","pages":"Article 109891"},"PeriodicalIF":3.7,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0