Biochemical Engineering Journal最新文献_第4页

Optimal porosity in zeolitic imidazole framework for cost-effective furfural removal in biomass hydrolysates 沸石型咪唑骨架的最佳孔隙度对生物质水解液中糠醛的经济高效去除

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-09 DOI: 10.1016/j.bej.2025.109858

Kanghong Wang , Chaozhong Xu , Wei Xiong, Shanshan Tong, Jia Ouyang, Xiaoli Gu

{"title":"Optimal porosity in zeolitic imidazole framework for cost-effective furfural removal in biomass hydrolysates","authors":"Kanghong Wang , Chaozhong Xu , Wei Xiong, Shanshan Tong, Jia Ouyang, Xiaoli Gu","doi":"10.1016/j.bej.2025.109858","DOIUrl":"10.1016/j.bej.2025.109858","url":null,"abstract":"<div><div>Efficient removal of fermentation inhibitors, particularly furan aldehydes like furfural is crucial for enhancing microbial fermentation efficiency in lignocellulosic biomass processing. In this study, zeolitic imidazolate framework-8 with different molar ratios were synthesized by regulating the Zn<sup>2 +</sup> /2-methylimidazole molar ratio (from 1:4–1:15), and their structural properties and adsorption performance were systematically evaluated. The results showed that the pore structure was crucial to the adsorption performance, and the pore structure was optimal when the Zn<sup>2+</sup>/2-methylimidazole ratio was 1:8. The optimized zeolitic imidazole framework-8 showed rapid, selective and efficient adsorption of furfural under different operating conditions, thereby preventing the formation of inhibitors from affecting fermentation while minimizing sugar loss. This study provides a generalizable design principle for the development of adsorbents in biomass conversion. This work provides a scalable solution for biomass hydrolysate detoxification, with potential cost advantages due to the material’s reusability and high adsorption capacity compared to conventional resin-based methods.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109858"},"PeriodicalIF":3.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144597066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zinc extraction from cast-house dust using the chemolithotrophic microorganism Acidithiobacillus thiooxidans 利用化化嗜硫微生物酸化硫杆菌从铸造厂粉尘中提取锌

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-08 DOI: 10.1016/j.bej.2025.109859

Rebeka Frueholz , Clemens Habermaier , Sabine Spiess , Ludwig Birklbauer , Georg M. Guebitz , Marianne Haberbauer

{"title":"Zinc extraction from cast-house dust using the chemolithotrophic microorganism Acidithiobacillus thiooxidans","authors":"Rebeka Frueholz , Clemens Habermaier , Sabine Spiess , Ludwig Birklbauer , Georg M. Guebitz , Marianne Haberbauer","doi":"10.1016/j.bej.2025.109859","DOIUrl":"10.1016/j.bej.2025.109859","url":null,"abstract":"<div><div>The continuous generation of steel by-products calls for innovative recycling solutions in order to strive towards a circular economy. In this study, the bioleaching potential of the chemolithotrophic microorganism <em>A. thiooxidans</em> was evaluated to extract unwanted Zn from the steel by-product cast-house dust (CHD) while specifically retaining Fe within the dust matrix to enable on-site recycling. A fed-batch stirred tank reactor was employed, and process stability was assessed through a series of replicates at a CHD concentration of 125 g L⁻¹ . Two distinct aerations 0.42 vvm and 0.50 vvm were examined during the course of this investigation. After 14 days, a maximum of 75 ± 5 % Zn underwent solubilization while only 9 ± 2 % of Fe was solubilized. However, after 7 days the majority of Zn was extracted resulting in an average leaching efficiency of 68 ± 2 % for Zn and 5 ± 2 % for Fe. Although increasing the aeration rate did not significantly improve the <em>k<sub>L</sub>a</em> value, it slightly enhanced O₂ availability, highlighting the need for further optimization of the aeration setup.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109859"},"PeriodicalIF":3.7,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144597067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel bioengineered spider silk-based particle for the delivery system of chemotherapy drugs 一种新型生物工程蜘蛛丝为基础的化疗药物递送系统颗粒

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-03 DOI: 10.1016/j.bej.2025.109855

Yongqiang Zhou , Lanxiao Shen , Zhimin Zheng , Mingrou Guo , Zheng Peng

{"title":"A novel bioengineered spider silk-based particle for the delivery system of chemotherapy drugs","authors":"Yongqiang Zhou , Lanxiao Shen , Zhimin Zheng , Mingrou Guo , Zheng Peng","doi":"10.1016/j.bej.2025.109855","DOIUrl":"10.1016/j.bej.2025.109855","url":null,"abstract":"<div><div>Spider silk protein (spidroin) is the primary constituent of spider silk fibers and exhibits remarkable biocompatibility, thermal stability, and biodegradability, making it a promising biomaterial for biomedical applications, particularly in drug delivery. Currently, drug delivery systems utilizing spidroins predominantly focus on engineered dragline spidroins. However, research on other spidroin types—such as aciniform spidroins (AcSp) —in their application as biomaterials within drug delivery systems remains very limited. Here, we engineered the recombinant spidroin AS2, derived from the <em>Araneus ventricosus</em> AcSp2 protein repeat domain, and designed a modified version, eAS2, by incorporating an additional glutamic acid residue. Both AS2 and eAS2 proteins exhibited self-assembly properties, forming non-toxic nanoparticles with similar secondary structure content in potassium phosphate buffer but differed in zeta potential values. Although eAS2 particles demonstrated high loading efficiency for positively charged drugs like mitoxantrone (MTX), a rapid release rate was also noted for MTX-loaded eAS2 particles. Conversely, the combination of efficient loading and a desirable slow release profile renders eAS2 particles an optimal delivery system for neutral and positive charged drugs, such as etoposide (ETP) and doxorubicin (DOX).</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109855"},"PeriodicalIF":3.7,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chalcone isomerase-like (CHIL) impedes the lactone shunt and modulates flux partitioning in isoflavonoid biosynthesis 查尔酮异构酶样（CHIL）阻碍内酯分流并调节异黄酮生物合成中的通量分配

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-03 DOI: 10.1016/j.bej.2025.109853

Lee Marie Raytek , Brandon Corey Saltzman , Meha Sharma , Soon Goo Lee , Mehran Dastmalchi

{"title":"Chalcone isomerase-like (CHIL) impedes the lactone shunt and modulates flux partitioning in isoflavonoid biosynthesis","authors":"Lee Marie Raytek , Brandon Corey Saltzman , Meha Sharma , Soon Goo Lee , Mehran Dastmalchi","doi":"10.1016/j.bej.2025.109853","DOIUrl":"10.1016/j.bej.2025.109853","url":null,"abstract":"<div><div>The reconstitution of biosynthetic pathways in heterologous hosts is often challenged by the transition to a foreign cellular environment, lacking compatible structural or regulatory features. Auxiliary or non-catalytic proteins can play a critical role in guiding metabolic flux. Chalcone isomerase-like (CHIL) is a non-catalytic protein known to serve as a partner to chalcone synthase (CHS) in flavonoid biosynthesis, rectifying its promiscuous activity and preventing the formation of by-products, such as <em>p</em>-coumaroyltriacetic acid lactone (CTAL). Here, we extended the functional analysis of CHILs to the legume-characteristic isoflavonoid pathway. We assessed CHIL orthologs, using sequence analysis and structural modelling to predict their respective binding capacities, followed by functional characterization. The addition of CHIL to enzyme assays containing CHS, alone or with downstream enzymes, chalcone reductase (CHR) and chalcone isomerase (CHI), reduced CTAL levels (up to 42 %) while simultaneously increasing the output of desired intermediates. Combinatorial yeast biotransformation assays revealed that CHIL plays a crucial role in directing metabolic flux through chalcone and flavanone pathways, and, with co-expression of isoflavone synthase (IFS), in isoflavone biosynthesis. The inclusion of CHIL in engineered yeast strains enhanced overall titers and, unexpectedly, promoted partitioning toward the deoxy-branch (isoliquiritigenin, liquiritigenin, and daidzein) up to 50 %, with a 33 % increase in final daidzein titers. Therefore, we have revealed an expanded role for CHIL as an auxiliary protein in isoflavonoid biosynthesis and underscored the utility of non-catalytic proteins in metabolic engineering.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109853"},"PeriodicalIF":3.7,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A cheap real-time optimal control setup for microalgae cultures: Simulation and experimental results 一种廉价的微藻培养实时最优控制装置：模拟和实验结果

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-02 DOI: 10.1016/j.bej.2025.109833

Jesús Miguel Zamudio Lara , Laurent Dewasme , Alain Vande Wouwer

引用次数: 0

Targeted expression and purification of elastin-like polypeptide-fused abrin: A potential strategy for cancer therapy 弹性蛋白样多肽融合abrin的靶向表达和纯化：一种潜在的癌症治疗策略

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-02 DOI: 10.1016/j.bej.2025.109850

Fariba Rafiei , Ali-Akbar Shahnejat-Bushehri , Fariba Abooie Mehrizi , Seyed Amirreza Sabzian , Houshang Alizadeh

{"title":"Targeted expression and purification of elastin-like polypeptide-fused abrin: A potential strategy for cancer therapy","authors":"Fariba Rafiei , Ali-Akbar Shahnejat-Bushehri , Fariba Abooie Mehrizi , Seyed Amirreza Sabzian , Houshang Alizadeh","doi":"10.1016/j.bej.2025.109850","DOIUrl":"10.1016/j.bej.2025.109850","url":null,"abstract":"<div><div>Abrin is a potent plant-derived toxin that targets 28S rRNA in eukaryotic ribosomes through its N-glycosidase activity. Its ability to induce apoptosis makes it a promising candidate for cancer-targeted immunotoxin therapy. To enhance the expression of Abrin in <em>E. coli</em>, its coding sequence was optimized by replacing native codons with synonymous codons that match codon usage preferences of <em>E. coli</em>, applying both codon usage frequency and codon harmonization strategies. The optimized sequence was generated using a two-step PCR process and cloned into expression vectors to produce both native and optimized unfused Abrin, as well as Elastin-Like Polypeptide fused forms. These included the constructs pET. native Abrin and pET. optimized Abrin (with His-tags), as well as pET. native Abrin-ELP and pET. optimized Abrin-ELP (with ELP tags). All constructs were successfully expressed in <em>E. coli</em>. Codon optimization led to approximately a two-fold increase in Abrin accumulation, resulting in stable expression levels comprising 70.72 % of total soluble protein. MTT assays demonstrated a concentration-dependent antiproliferative effect of Abrin on LS180 and MCF-7 cancer cells. It is noteworthy that the ELP-fused proteins significantly increased cytotoxic activity in LS180 cells, with IC<sub>50</sub> values of 5.329 µg/mL for native Abrin-ELP and 4.828 µg/mL for the optimized Abrin-ELP. These findings highlight the therapeutic potential of ELP-fused Abrin as a targeted cancer treatment.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109850"},"PeriodicalIF":3.7,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144570772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing a scalable framework for mitigating GEV and dissolved CO2 stresses in high-density CHO cell cultures 开发一个可扩展的框架，以减轻高密度CHO细胞培养中的GEV和溶解的CO2压力

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-02 DOI: 10.1016/j.bej.2025.109851

Dan Xu , Yanpeng Ci , Ying Hou , Le Yu , Jun Tian , Haozhe Ruan , Yina Shen , Jingjing Zhang , Huifen Yu , Hanyi Wang , Ming Zhang , Guangyao Hu , Muchen Li , Tianli Zhang , Yongsheng Xiao , Vadim Tsvetnitsky , Jun Luo , Sherry Gu , Weichang Zhou

{"title":"Developing a scalable framework for mitigating GEV and dissolved CO2 stresses in high-density CHO cell cultures","authors":"Dan Xu , Yanpeng Ci , Ying Hou , Le Yu , Jun Tian , Haozhe Ruan , Yina Shen , Jingjing Zhang , Huifen Yu , Hanyi Wang , Ming Zhang , Guangyao Hu , Muchen Li , Tianli Zhang , Yongsheng Xiao , Vadim Tsvetnitsky , Jun Luo , Sherry Gu , Weichang Zhou","doi":"10.1016/j.bej.2025.109851","DOIUrl":"10.1016/j.bej.2025.109851","url":null,"abstract":"<div><div>Intensified fed-batch featuring high-cell-density cultures highlights challenges in bioreactor scale-up and underscores the needs for tailored mitigation, particularly on the reconciliation of the hydrodynamic stress from sparger gas entrance velocity (GEV) and accumulated CO₂ partial pressure (pCO₂). In this study, elevated GEV and pCO₂ were identified as the causes of titer reduction during the scale-up to a 2000 L single-use bioreactor (SUB). To verify this hypothesis, a scaled-down model was established in bench-scale bioreactors equipped with a customized sparger to simulate the high GEV and pCO₂ conditions. The model reproduced the impaired titer performance, showing that both factors inhibited production through an independent mechanism and a culture phase-dependent manner. Proteomic analysis of the two stresses further revealed differentially protein expressions associated with cell proliferation, energy generation and reactive oxygen species induced cellular responses. Lastly, an optimized scale-up strategy balancing effective pCO<sub>2</sub> stripping with controlled GEV stress was implemented through a modified 2000 L SUB with an upgraded sparger design. This work elucidated the interplay between elevated pCO<sub>2</sub> and GEV stresses and culture performance, establishing a systematic framework for modulating GEV and pCO₂ as a universal scale-up tool. The findings advanced scale-up strategies for all mammalian cell cultures, especially for shear stress- and/or pCO₂-sensitive cell lines, while reinforcing mechanistic insights into bioprocess scalability for industrial applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109851"},"PeriodicalIF":3.7,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Waste valorization strategy: Fabrication of high-efficiency magnetic ultrafine cattle hair powder and its adsorption performance on acid dye 废物增值策略：高效磁性超细牛毛粉的制备及其对酸性染料的吸附性能

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-01 DOI: 10.1016/j.bej.2025.109847

Xinyue Nian , Feifei Zhang , Jie Liu , Jianan Song

{"title":"Waste valorization strategy: Fabrication of high-efficiency magnetic ultrafine cattle hair powder and its adsorption performance on acid dye","authors":"Xinyue Nian , Feifei Zhang , Jie Liu , Jianan Song","doi":"10.1016/j.bej.2025.109847","DOIUrl":"10.1016/j.bej.2025.109847","url":null,"abstract":"<div><div>To address the environmental issues caused by the accumulation of large amounts of animal hair generated during the leather production process, this study mechanically milled cattle hair waste (CHW) into ultrafine cattle hair powder (UCP), and a magnetic cattle hair powder adsorbent material (MUCP) was synthesized through an in-situ generation method using FeSO<sub>4</sub>·7 H<sub>2</sub>O and FeCl<sub>3</sub>·6 H<sub>2</sub>O as sources. Adsorption experiments were conducted under various conditions to investigate the adsorption performance of MUCP for acid dye. The results showed that lower pH levels led to higher dye removal rates. When the pH was 2, the dye removal rate reached 93.1 %. The adsorption process was consistent with the pseudo-second-order kinetics model and the Langmuir isotherm model. When 0.1 g of MUCP-8 was used to adsorb the dye solution with an initial concentration of 500 mg·L<sup>−1</sup>, pH 3, 45 ℃, and a duration time of 48 h, the maximum adsorption capacity was 699.30 mg·g<sup>−1</sup>. The adsorption mechanism of MUCP for acid dye mainly involved void filling, hydrogen bonding, and electrostatic attraction between protonated amino groups and negatively charged dye ions. MUCP has superparamagnetic properties, allowing easy and quick separation and recovery from the mixed solution under an external magnetic field after dye adsorption. This provides a new solution for bio-based adsorbent materials with magnetic properties in dye wastewater treatment and waste-to-resource methods.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109847"},"PeriodicalIF":3.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xylonic acid: A novel approach to bioproduction by sustainable strategies 木糖酸：一种可持续发展的生物生产新方法

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-30 DOI: 10.1016/j.bej.2025.109846

Caroline Ribeiro Corrêa, Sabrina Carra, Vanderson Antônio de Lima, Camila Klein, João Vitor Faccin Barbosa, Eloane Malvessi

{"title":"Xylonic acid: A novel approach to bioproduction by sustainable strategies","authors":"Caroline Ribeiro Corrêa, Sabrina Carra, Vanderson Antônio de Lima, Camila Klein, João Vitor Faccin Barbosa, Eloane Malvessi","doi":"10.1016/j.bej.2025.109846","DOIUrl":"10.1016/j.bej.2025.109846","url":null,"abstract":"<div><div>Despite its recognized potential use in polyamides, cosmetic formulations, hydrogels, and as an antimicrobial, the sustainable bioproduction of xylonic acid remains underestimated in the field of industrial biotechnology. In parallel, sorbitol, a high added-value polyol widely used in the food and pharmaceutical industries, can also be obtained through biotechnological routes. Both compounds can be synthesized from xylose and fructose through the coordinated enzymatic action of glucose-fructose oxidoreductase (GFOR) and glucono-δ-lactonase (GL), periplasmic enzymes produced by <em>Zymomonas mobilis</em> cells. In this study, the activity of the GFOR/GL complex was evaluated under different pH (5.8–7.6) and temperature (34–53 °C) conditions, using 700 mmol/L of xylose/fructose as substrates and 4 g/L of biocatalyst. The optimum activity conditions were identified between pH 6.8–7.2 and 47–50 °C. Subsequent bioconversion trials demonstrated impressive xylonic acid yields of 90 % at 39 °C and pH 6.4 using free enzymes, and 89 % at 43 °C and pH 6.4 when enzymes were immobilized in calcium alginate. Reusability tests revealed the immobilized biocatalyst's stable performance over five cycles, exhibiting no significant loss of efficiency in xylonic acid production. These findings underscore the potential of the GFOR/GL system as an efficient and sustainable alternative for valorizing renewable sugars into high-value industrial products.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109846"},"PeriodicalIF":3.7,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144535883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Granular activated carbon enhances microbial activity in anaerobic reactors: Insights from metagenomics and metaproteomics 颗粒活性炭增强厌氧反应器中的微生物活性：来自宏基因组学和宏蛋白质组学的见解

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-06-28 DOI: 10.1016/j.bej.2025.109843

Carlo Bais , Yingdi Zhang , Qi Huang , Chelsea Benally , Yang Liu

{"title":"Granular activated carbon enhances microbial activity in anaerobic reactors: Insights from metagenomics and metaproteomics","authors":"Carlo Bais , Yingdi Zhang , Qi Huang , Chelsea Benally , Yang Liu","doi":"10.1016/j.bej.2025.109843","DOIUrl":"10.1016/j.bej.2025.109843","url":null,"abstract":"<div><div>Granular activated carbon (GAC) enhances anaerobic digestion (AD) primarily by promoting direct interspecies electron transfer (DIET). However, as most biomass in bioreactors is suspended rather than attached, GAC may also play additional roles in stimulating suspended biomass beyond DIET. In this study, two lab-scale up-flow anaerobic sludge blanket (UASB) reactors were operated for 150 days with propionate-rich synthetic wastewater, one of which was amended with GAC to investigate its broader effects on microbial activity and metabolic function. Results showed that GAC addition significantly improved chemical oxygen demand (COD) removal (92.1 ± 5.0 %) and methane yield (70.3 ± 8.2 %) compared to the non-GAC reactor (81.0 ± 2.1 % and 55.4 ± 5.2 %). Metagenomic analysis revealed a shift toward hydrogenotrophic methanogenesis, with an increased abundance of <em>Methanobacterium sp.</em> (31.4 %). Metaproteomic profiling and functional gene prediction indicated elevated expression of proteins involved in methanogenesis (e.g., methyl-coenzyme M reductase), energy metabolism (e.g., ATP synthase), and cofactor biosynthesis (e.g., CobS and CobT enzymes). Additionally, batch tests using reactor effluents demonstrated that the GAC-amended system contained active substances capable of stimulating methane production, indicating the release of bioavailable metabolites. These findings suggest that GAC enhances microbial activity not only by facilitating DIET but also by stimulating the biosynthesis of key functional proteins and cofactors. This understanding supports the development of GAC-enhanced anaerobic systems for more stable and efficient reactors in full-scale applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109843"},"PeriodicalIF":3.7,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0