Biochemical Engineering Journal最新文献_第4页

Three-dimensional and serum-free culture in fixed-bed bioreactor enhance exosome production by affecting the cytoskeleton through integrin β1 and RAC1 固定床生物反应器三维和无血清培养通过整合素β1和RAC1影响细胞骨架，促进外泌体的产生

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-11 DOI: 10.1016/j.bej.2025.109750

Chuanfu Huo , Yuanyuan Zhao , Meng Song , Yuzhe Guo , Shihao Li , Wensong Tan , Yan Zhou

{"title":"Three-dimensional and serum-free culture in fixed-bed bioreactor enhance exosome production by affecting the cytoskeleton through integrin β1 and RAC1","authors":"Chuanfu Huo , Yuanyuan Zhao , Meng Song , Yuzhe Guo , Shihao Li , Wensong Tan , Yan Zhou","doi":"10.1016/j.bej.2025.109750","DOIUrl":"10.1016/j.bej.2025.109750","url":null,"abstract":"<div><div>Exosomes are nanoscale vesicles containing a variety of bioactive factors, showing significant potential as cell-free therapeutic agents. However, the large clinical doses required for exosome administration highlight the need for a scalable serum-free production system to increase exosome yield. In this study, human umbilical cord-derived mesenchymal stem cells (hUCMSCs) were cultured in three-dimensional (3D) environments using polyethylene terephthalate (PET) fiber membranes. Two key pathways promoting exosome secretion and biogenesis through 3D culture were identified. Specifically, 3D culture reduced the expression of Integrin β1, leading to the decondensation of cortical actin, and increased the expression of RAC1, which promoted actin aggregation on the membranes of multivesicular bodies (MVBs). Building on this mechanistic understanding, we simulated the depolymerization/aggregation of the cytoskeleton in 3D culture and designed a serum-free medium optimized for exosome production. For large-scale exosome production, hUCMSCs were cultured in a fixed-bed bioreactor using PET fiber rolls as the fixed-bed layer with the developed serum-free medium. This setup increased exosome production per cell by 16-fold compared to 2D culture, yielding 2.6 × 10^14 exosome particles. The exosome products demonstrated enhanced <em>in vitro</em> angiogenesis, immunomodulation, and <em>in vivo</em> wound healing capabilities. This study provides valuable insights and strategic guidance for the serum-free, large-scale production of exosomes using bioreactors, with significant implications for therapeutic applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109750"},"PeriodicalIF":3.7,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Designing and fabrication of BIRC5-mRNA-based biosensor for early breast cancer diagnosis using a nanostructured (CeO2-Ru@AuNPs-CNTs)-lab-on-chip 基于birc5 - mrna的早期乳腺癌纳米传感器的设计与制造（CeO2-Ru@AuNPs-CNTs）

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-10 DOI: 10.1016/j.bej.2025.109754

Engy M. Madkour , Mohamed Abdel-Hakeem , Sahar A. Fadlallah , Sherif Abdelaziz Ibrahim , Rabeay Y.A. Hassan

{"title":"Designing and fabrication of BIRC5-mRNA-based biosensor for early breast cancer diagnosis using a nanostructured (CeO2-Ru@AuNPs-CNTs)-lab-on-chip","authors":"Engy M. Madkour , Mohamed Abdel-Hakeem , Sahar A. Fadlallah , Sherif Abdelaziz Ibrahim , Rabeay Y.A. Hassan","doi":"10.1016/j.bej.2025.109754","DOIUrl":"10.1016/j.bej.2025.109754","url":null,"abstract":"<div><div>Baculoviral Inhibitor of Apoptosis Repeat Containing 5 (BIRC5) gene, encoding the protein survivin, has garnered substantial research interest. Survivin is overexpressed in various cancers, including breast cancer, making BIRC5 a crucial biomarker for the early diagnosis and prognosis. Thus, a portable and disposable electrochemical biosensing system was designed and applied for the sensitive diagnosis of breast cancer targeting the BIRC5-mRNA. In this regard, gold nanoparticles assisted the formation of a self-assembling monolayer of the crosslinking chain (4-Aminothiophenol (ATP)) on the top of the nanocomposite layer that contains cerium oxide (CeO<sub>2</sub>)-Ruthenium (Ru)@multiwall carbon nanotubes (MWCNTs).Subsequently, the single stranded probe complementary to BIRC5-mRNA was chemically immobilized. Selectivity and sensitivity features of the newly designed BIRC5-mRNA-based biosensor were determined through a full assay optimization, whereas a detection limit of 0.4 ag/mL was successfully achieved. The newly developed nanostructured BIRC5 mRNA biosensor could be applied to provide a rapid and direct determination of the targeting BIRC5 mRNA in the patients’ blood samples without any additional labeling step that may be required in currently held RNA biosensors.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109754"},"PeriodicalIF":3.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased labyrinthopeptin A1 production is associated with changes in oxygen supply, density and elasticity of Actinomadura namibiensis pellets 迷宫肽A1生成的增加与纳米比亚放线菌颗粒的供氧、密度和弹性的变化有关

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-10 DOI: 10.1016/j.bej.2025.109747

Zuzanna Justyna Kozanecka , Jintian Liu , Qiyue Liu , Hannah Buch , Jona Gebauer , Detlev Rasch , Markus Böl , Rainer Krull

{"title":"Increased labyrinthopeptin A1 production is associated with changes in oxygen supply, density and elasticity of Actinomadura namibiensis pellets","authors":"Zuzanna Justyna Kozanecka , Jintian Liu , Qiyue Liu , Hannah Buch , Jona Gebauer , Detlev Rasch , Markus Böl , Rainer Krull","doi":"10.1016/j.bej.2025.109747","DOIUrl":"10.1016/j.bej.2025.109747","url":null,"abstract":"<div><div>Labyrinthopeptin A1, a promising broad-spectrum antiviral, is produced exclusively by the filamentous actinomycete <em>Actinomadura namibiensis</em>. In submerged cultures of <em>A. namibiensis</em>, supplementation with 50 mM ammonium sulfate resulted in a 6.3-fold increase in product formation, accompanied by enhanced glycerol consumption, lower dissolved oxygen tension, and changes in pellet morphology. This study aims to elucidate the underlying mechanisms of this bioprocess intensification method using novel tools such as oxygen microprofiling, plate-plate compression, and sedimentation experiments to investigate pellet characteristics possibly associated with product formation. Oxygen microprofiling revealed steeper profiles in salt-supplemented pellets, indicating heightened metabolic activity and potential oxygen limitation in pellet cores during exponential growth phase, which affects the subsequent secondary metabolite production. For the first time, pellet density was estimated using sedimentation experiments. While control pellets showed no density trends, salt-supplemented pellets became more porous over time, suggesting a link to the increased glycerol uptake during product formation. Additionally, the compression experiments showed greater increase in stiffness in salt-supplemented pellets over time, suggesting the development of stiffer structures within the hyphal network and increased pellet stability. This observation was previously inferred only through image analysis and cell dry weight concentration. Ultimately, the results presented here contribute to the development of numerical simulations and predictive models for the productivity and growth behavior of filamentous pellet cultures in the future.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109747"},"PeriodicalIF":3.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143828671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photochemical synthesis of polymer-stabilized silver nanoparticles for epinephrine sensing: Electrochemical, EG-FET and microcontroller based approaches 用于肾上腺素传感的聚合物稳定银纳米粒子的光化学合成：电化学，EG-FET和基于微控制器的方法

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-10 DOI: 10.1016/j.bej.2025.109755

Chandan Saha , Pooja Kumari , Lungelo Mgenge , Sarit K. Ghosh , Harishchandra Singh , Kaushik Mallick

{"title":"Photochemical synthesis of polymer-stabilized silver nanoparticles for epinephrine sensing: Electrochemical, EG-FET and microcontroller based approaches","authors":"Chandan Saha , Pooja Kumari , Lungelo Mgenge , Sarit K. Ghosh , Harishchandra Singh , Kaushik Mallick","doi":"10.1016/j.bej.2025.109755","DOIUrl":"10.1016/j.bej.2025.109755","url":null,"abstract":"<div><div>Epinephrine, a key catecholamine, regulates the sympathetic nervous system and plays a vital role in various physiological processes. This study presents various sensor applications for epinephrine detection, based on a polymer-stabilized silver nanoparticle composite catalyst, synthesized through a UV-mediated route. The metal-polymer composite system was characterized by transmission electron microscopy, X-ray diffraction, Raman and X-ray photoelectron spectroscopy techniques. The electrochemical redox behavior of epinephrine in the presence of the catalyst was studied using various voltametric and amperometric techniques. The electrocatalyst demonstrated high selectivity towards epinephrine detection in the presence of common biological interfering analytes like dopamine, uric acid and ascorbic acid. A sensing technique based on an extended gate field-effect transistor (EG-FET) was applied for epinephrine detection, achieving a limit of detection of 0.72 μM and a sensitivity of 0.049 μA.μM<sup>−1</sup>.cm<sup>−2</sup>. A microcontroller-based portable sensor was developed using an Arduino Uno R4 Wi-Fi module, enabling real-time monitoring and remote data transmission. The sensitivity and selectivity of the sensor make it a promising tool for on-site epinephrine detection in clinical and research applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109755"},"PeriodicalIF":3.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensitive indicator microorganisms and C,N-cycle processes in soil with different petroleum hydrocarbon pollution levels 不同石油烃污染程度土壤中敏感指示微生物及C、n循环过程

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-09 DOI: 10.1016/j.bej.2025.109752

Ting Zhang , Xuhong Zhang , Zeliang Liu , Yawen Ou , Xuhong Duan , Manli Wu

{"title":"Sensitive indicator microorganisms and C,N-cycle processes in soil with different petroleum hydrocarbon pollution levels","authors":"Ting Zhang , Xuhong Zhang , Zeliang Liu , Yawen Ou , Xuhong Duan , Manli Wu","doi":"10.1016/j.bej.2025.109752","DOIUrl":"10.1016/j.bej.2025.109752","url":null,"abstract":"<div><div>Total petroleum hydrocarbon(TPH) is toxic to soil microorganisms and humans and has become a serious public concern. This study employed chemical analysis, high-throughput sequencing, and KEGG database functional gene annotation to investigate microbial responses to TPH contamination and identify potential bioindicators for soil toxicity assessment. Our results demonstrated that TPH stress significantly altered soil microbial community stability and functional structure. In contaminated soils, the relative abundance of <em>Actinomycetes</em> increased by 14.03–25.06 %, while <em>Ascomycetes</em> and <em>Mortieres</em> increased by 0.85–2.2 % and 1.62–2.39 %, respectively, compared to uncontaminated soil. The Mantel test analysis identified that <em>Nocardioides, unclassified-Micrococcaceae, Streptomyces,</em> and <em>Gibberella</em> as robust bioindicators petroleum contamination(<em>p</em> < 0.05). Network analysis revealed that petroleum hydrocarbons enhanced microbial community complexity, with network edges increasing by 20–90 % in contaminated soils. PICRUSt2 functional prediction showed that TPH pollution altered microbial metabolic pathways, with bacterial metabolic processes and fungal biosynthesis processes dominating (>60 %) the total pathways. The abundance of nitrification genes involved in nitrogen transformation was positively correlated with soil oil content, denitrification, and nitrogen-fixing genes showed a negative correlation with TPH content. Expressions of functional genes involved in carbon photosynthesis fixation are inhibited in oil-contaminated soil with the hydroxypropanoate-hydroxybutylate cycle (M00375) being the main carbon cycle pathway.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109752"},"PeriodicalIF":3.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of different influent carbon-to-phosphorus ratios on phosphorus transformation, microbial community structure and metabolic functions in an upflow anaerobic sludge bed reactor 不同进水碳磷比对上流式厌氧污泥床反应器中磷转化、微生物群落结构和代谢功能的影响

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-08 DOI: 10.1016/j.bej.2025.109740

Jiahao Chen , Afeng Tao , Qiuhong Li , Zhengpeng Chen , Ziyi Wu , Xiaoyue Liu , Shenglong Chen , Yuxiang Lu , Yayuan Mo , Chengyuan Su

{"title":"Effects of different influent carbon-to-phosphorus ratios on phosphorus transformation, microbial community structure and metabolic functions in an upflow anaerobic sludge bed reactor","authors":"Jiahao Chen , Afeng Tao , Qiuhong Li , Zhengpeng Chen , Ziyi Wu , Xiaoyue Liu , Shenglong Chen , Yuxiang Lu , Yayuan Mo , Chengyuan Su","doi":"10.1016/j.bej.2025.109740","DOIUrl":"10.1016/j.bej.2025.109740","url":null,"abstract":"<div><div>Excessive phosphorus emissions can result in the eutrophication of water bodies, causing severe environmental damage as well as influencing the efficiency of water treatment equipment. The impacts of carbon/phosphorus ratios on performance and mechanism of the upflow anaerobic sludge bed reactor remain unclear. Henrie, the effects of different carbon/phosphorus ratios (i.e., 80:1, 40:1, and 20:1) on the transformation of phosphorus in the biological treatment process of an upflow anaerobic sludge blanket (UASB) reactor were studied. The results showed that phosphines are of great importance in the phosphate reduction process. After a stable operation, the phosphine reached the highest 81.91 mg/m<sup>3</sup> at a C/P ratio of 40:1. It was proved that the optimum operating condition of the reactor was carbon to phosphorus ratio of 40:1. Phosphate-reducing bacteria were present in the UASB reactor, and the relative abundance of <em>Clostridia</em> in the sludge was 1.90 % and 1.59 % when the C/P was 80:1 and 20:1, respectively. This implied that the low carbon to phosphorus ratio reduces the phosphorus-reducing microbial activity in the reactor. Lower C/P values could inhibit the uptake and use of P in the phosphonate transport system and the transport of phosphate in the cell by the microbial <em>Pst</em> system, impeding the mineralization of organophosphates. The study provides new insights into improving the efficiency of treating phosphorus-rich wastewater.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109740"},"PeriodicalIF":3.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ammonia nitrogen degradation metabolic pathway by the novel strain Paracoccus sp. TD-10 and its molecular mechanisms 新菌株副球菌sp. TD-10的氨氮降解代谢途径及其分子机制

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-08 DOI: 10.1016/j.bej.2025.109751

Ying Lei, Meimei Wan, Peiyang Zheng, Da Ao, Wenlong Yue, Zhiqiang Cai

{"title":"Ammonia nitrogen degradation metabolic pathway by the novel strain Paracoccus sp. TD-10 and its molecular mechanisms","authors":"Ying Lei, Meimei Wan, Peiyang Zheng, Da Ao, Wenlong Yue, Zhiqiang Cai","doi":"10.1016/j.bej.2025.109751","DOIUrl":"10.1016/j.bej.2025.109751","url":null,"abstract":"<div><div>Heterotrophic nitrification-aerobic denitrification（HN-AD） bacteria have attracted significant attention due to their high tolerance for ammonia nitrogen, efficient ammonia nitrogen degradation capabilities, and strong environmental adaptability in removing nitrogen contaminants from residential and industrial wastewater. This study aimed to investigate the ammonia nitrogen degradation characteristics of a novel HN-AD strain with superior ammonia nitrogen degradation capacity, providing a foundation for optimizing the ammonia nitrogen degradation processes in wastewater treatment facilities. The ammonia nitrogen degradation ability of <em>Paracoccus sp.</em> TD-10 was evaluated under specific conditions: pH 9.0, 28 ℃ and an initial concentration of 100.0 mg/L NH₄⁺, with sodium acetate serving as both the carbon and energy source. The results demonstrated a 100 % degradation rate under these conditions. Whole-genome analysis, functional gene identification, and nitrogen balance studies suggested a potential ammonia degradation pathway: NO₃⁻-N → NO₂⁻-N → NO → N₂O → N₂, and NH₄⁺-N → Gln → Glu. The exceptional ammonia nitrogen degradation efficiency of strain TD-10, combined with its ability to prevent the accumulation of nitrite and nitrate nitrogen, offers a solid theoretical basis for optimizing HN-AD bacteria in the sewage ammonia nitrogen degradation process.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109751"},"PeriodicalIF":3.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ production of single-cell protein in microbial electrochemical systems via controlling the operation and CO2 addition 通过控制操作和二氧化碳添加，在微生物电化学系统中原位生产单细胞蛋白质

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-04-03 DOI: 10.1016/j.bej.2025.109738

Qi Yang , Xuejiao Qi , Kai Luo , Rongbo Guo , Dongmei Wang , Shanfei Fu

{"title":"In situ production of single-cell protein in microbial electrochemical systems via controlling the operation and CO2 addition","authors":"Qi Yang , Xuejiao Qi , Kai Luo , Rongbo Guo , Dongmei Wang , Shanfei Fu","doi":"10.1016/j.bej.2025.109738","DOIUrl":"10.1016/j.bej.2025.109738","url":null,"abstract":"<div><div>Recovering both energy and nitrogen from livestock wastewater through microbial electrochemical cell (MEC) for the production of single-cell proteins (SCP) represents a promising technology for microbial protein generation. However, the high pH in the MEC cathode restricts the growth of microbial protein. In this study, intermittent operation of MEC and microbial fuel cell (MFC), along with the addition of CO<sub>2</sub>, was performed to overcome the limiting factor of high cathode pH, thus achieving the SCP production in situ by recovering both energy and ammonia nitrogen. The removal efficiencies of COD and ammonia nitrogen in wastewater were up to 95 % and 50 %, respectively. The produced protein concentration could reach 67 %, and the dry weight was 168.3 mg·L<sup>−1</sup>. Seven essential and ten non-essential amino acids were detected, and the essential amino acid richness in the protein was 31 %, surpassing that of meat. After simplified economic evaluation, the economic potential of the electrochemical system is 1.35 €/m<sup>3</sup>. Additionally, the electron acceptance behavior of <em>Cupriavidus necator</em> H16 from cathode when the pH is around 8.0 was proposed, which supported the production of SCP in MFC. This study provides a new concept for further promoting the resource utilization of livestock wastewater and developing an energy- and resource-efficient process for protein production.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109738"},"PeriodicalIF":3.7,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143792671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liquid paraffin gas carrier enhanced the HOB microbial protein production in a bubble column reactor with a gas-liquid mixing pump 液体石蜡气载体在气液混合泵的气泡塔反应器中提高了HOB微生物蛋白的产量

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-31 DOI: 10.1016/j.bej.2025.109737

Wenfang Cai , Xiaoying Shen , Jiayao Gao , Bingyan Wang , Xueqi Wang , Yaze Li , Jinzhe Liu , Yunhai Wang , Kun Guo

{"title":"Liquid paraffin gas carrier enhanced the HOB microbial protein production in a bubble column reactor with a gas-liquid mixing pump","authors":"Wenfang Cai , Xiaoying Shen , Jiayao Gao , Bingyan Wang , Xueqi Wang , Yaze Li , Jinzhe Liu , Yunhai Wang , Kun Guo","doi":"10.1016/j.bej.2025.109737","DOIUrl":"10.1016/j.bej.2025.109737","url":null,"abstract":"<div><div>Microbial protein (MP) production through autotrophically hydrogen-oxidizing bacterium (HOB) has been regarded as a potential technology for feed or food supply. However, due to the low solubility of O<sub>2</sub> and H<sub>2</sub>, how to match the biotic O<sub>2</sub> and H<sub>2</sub> consumption rate and the transfer kinetics presents the key challenge to achieve desired HOB protein production. Here, we introduce liquid paraffin as a gas carrier in a bubble column reactor with a self-priming gas-liquid mixing pump for HOB MP production. The self-priming gas-liquid mixing pump facilitates the efficiency of gas-liquid mixing, while the liquid paraffin improves the solubility of O<sub>2</sub> and H<sub>2</sub>. This combined effect optimizes the kinetics of O₂ and H₂ transfer, resulting in an increase in the K<sub>L</sub>a from 0.69 to 1.81 h<sup>−1</sup> for O<sub>2</sub>, and from 0.37 to 1.67 h<sup>−1</sup> for H<sub>2</sub>. Consequently, the yield of NH<sub>4</sub><sup>+</sup>-N to HOB protein increased from 4.21 ± 0.18 gMP∙gN<sup>−1</sup> to 5.18 ± 0.68 gMP∙gN<sup>−1</sup>, and an average protein content of 53 ± 5 % in dried biomass was achieved with nearly 100 % O<sub>2</sub> and H<sub>2</sub> uptake after adding liquid paraffin. The essential amino profile analysis shows that the presence of liquid paraffin has no remarkably impact on the protein quality. These results demonstrate that integration of gas carrier and reactor design is viable to address the gas transfer limitation in the MP production.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"219 ","pages":"Article 109737"},"PeriodicalIF":3.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New strategy for the design and cloning of novel humanized biparatopic anti-CD52 nanobodies: Tools for targeted drug delivery and therapeutic approaches 设计和克隆新型人源双异位抗cd52纳米体的新策略：靶向药物传递和治疗方法的工具

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-03-29 DOI: 10.1016/j.bej.2025.109736

Mohammad Mehdi Heidari , Mehri Khatami , Amir Soltanzade , Soroor Rezaei , Elham Mirhashemi

{"title":"New strategy for the design and cloning of novel humanized biparatopic anti-CD52 nanobodies: Tools for targeted drug delivery and therapeutic approaches","authors":"Mohammad Mehdi Heidari , Mehri Khatami , Amir Soltanzade , Soroor Rezaei , Elham Mirhashemi","doi":"10.1016/j.bej.2025.109736","DOIUrl":"10.1016/j.bej.2025.109736","url":null,"abstract":"<div><div>Immunotherapy has been developed to enhance the effectiveness of targeted cancer treatments. CD52 as the appropriate cell surface marker is a glycoprotein anchored to the cell membrane. It is found in large quantities in cancerous lymphoid cells, particularly in B and T cells, making it a suitable target for such therapies. Anti-CD52 antibodies can induce cell lysis through complement activation and direct cell-mediated cytotoxicity. However, the immunogenicity of antibodies limits their application in human disease. In contrast, the distinct properties of nanobodies make them ideal tools for targeted therapy. Here, we developed a novel humanized biparatopic nanobody targeting CD52, referred to as huCD52. We grafted the amino acid sequences of the CDRs from Alemtuzumab (1ce1) and Gatralimab (6obd) into a general humanized nanobody scaffold (Caplacizumab). We also conducted molecular docking analysis and molecular dynamics simulations. The nanobody gene fragment was cloned into the pET-21b(+) vector, expressed in <em>E. coli</em>, and subsequently purified. We evaluated the binding activity of the nanobody using the ELISA test. Human biparatopic huCD52 nanobody showed a strong binding affinity to CD52 protein. Our approach is a versatile alternative to traditional protocols, which usually require immobilization or complex selection methods. We have developed a platform for constructing high-affinity, biparatopic nanobody-based constructs, which has the potential to accelerate the development of therapeutic interventions targeting CD52 in antigen-presenting leukemia.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"219 ","pages":"Article 109736"},"PeriodicalIF":3.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0