Biochemical Engineering Journal最新文献_第4页

Development of inducible packaging cell line for rAAV production via CRISPR-Cas9 mediated site-specific integration 通过 CRISPR-Cas9 介导的位点特异性整合，开发用于生产 rAAV 的可诱导包装细胞系

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-31 DOI: 10.1016/j.bej.2024.109552

Qiang Fu , Yongdan Wang , Emily Doleh , Mark Blenner , Seongkyu Yoon

{"title":"Development of inducible packaging cell line for rAAV production via CRISPR-Cas9 mediated site-specific integration","authors":"Qiang Fu , Yongdan Wang , Emily Doleh , Mark Blenner , Seongkyu Yoon","doi":"10.1016/j.bej.2024.109552","DOIUrl":"10.1016/j.bej.2024.109552","url":null,"abstract":"<div><div>AAV-mediated gene therapy is a quickly growing segment of the pharmaceutical market; however, the current transient transfection process to produce rAAV has several challenges. The stable cells are ideal for large-scale continuous production, overcoming the drawbacks in the current transient transfection and streamlining rAAV production. In this study, we proposed to use synthetic inducible promoters to control the viral component expression and develop the baseline of HEK293T stable cells via site-specific integration mediated with CRISPR-Cas9, targeting safe harbor sites of human genome (ROSA26, AAVS1, and CCR5 locus). With a total of three round integrations, stable cell pools were developed and evaluated at each round of integration. Single clones were further characterized for each integration round. Regarding the stable pools, the 5’ and 3’ junction PCR results confirmed the site-specific integration to each locus. The genome copy result showed that AAV components, including Rep78/68, E2A, E4orf6, Cap, and Rep52/40, were successfully integrated into the host cell genome. Genome and capsid titer after induction confirmed rAAV production for stable cell pools in each round. The packaging cell line (after 2nd round integration) was able to produce rAAV. However, it was observed that the genome titer was ten-fold lower than that of rAAV products done with triple plasmids transfection. The out-to-out PCR and qPCR assay results further confirm the site-specific integration. This research demonstrates the feasibility of developing the inducible stable cell line with the refactored viral vectors via a site-specific integration.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109552"},"PeriodicalIF":3.7,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142586055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of tetracycline on mixotrophic denitrification process under different sulfur to nitrogen ratios 不同硫氮比条件下四环素对混养反硝化过程的影响

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-31 DOI: 10.1016/j.bej.2024.109557

Bohan Lv , Yang-Guo Zhao , Yue Chen , Mupindu Progress , Mengchun Gao , Liang Guo , Junyuan Ji , Chunji Jin

{"title":"Impact of tetracycline on mixotrophic denitrification process under different sulfur to nitrogen ratios","authors":"Bohan Lv , Yang-Guo Zhao , Yue Chen , Mupindu Progress , Mengchun Gao , Liang Guo , Junyuan Ji , Chunji Jin","doi":"10.1016/j.bej.2024.109557","DOIUrl":"10.1016/j.bej.2024.109557","url":null,"abstract":"<div><div>The sulfur-based autotrophic-heterotrophic denitrification, i.e., mixotrophic denitrification, is suitable for the nitrate and antibiotics removal in aquaculture tailwater at a low COD to nitrogen (C/N) ratio. This study focused on the effect of tetracycline (TC) on mixotrophic denitrification under different S/N ratios. Two bioreactors were simultaneously operated with or without dosing tetracycline under different sulfur to nitrogen (S/N) ratios of 3.94, 4.64 and 5.94. The results showed that the removal rate of total inorganic nitrogen (TIN) increased from 0.25 to 0.69 mg N L<sup>−1</sup> min<sup>−1</sup> with the rise of S/N ratio, while TC dosage significantly declined the removal efficiency of TIN. Dissimilatory nitrogen reduction to ammonia (DNRA) bacteria was detected when exposing to TC, indicating that DNRA presented more resistance to TC. The removal efficiency of TC in the denitrification system reached the maximum of 22.87 % at S/N of 4.64. Meanwhile the genus <em>Marinicella</em> was detected at this phase, which was conducive to the degradation of organic pollutants. This study found that TC promoted the accumulation of ammonia nitrogen, and had a great effect on sulfur autotrophic bacteria at S/N of 5.94. The removal of TC mainly depended on microbial co-metabolism, and there was a significant correlation between the reduction of TC concentration and the decrease of sulfur compounds (p < 0.05). 4.64 is the best S/N ratio for the mixotrophic denitrification process, which revealed maximum nitrate and TC removal rates.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109557"},"PeriodicalIF":3.7,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142577866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pros and cons of airlift and bubble column bioreactors: How internals improve performance 气浮和气泡柱生物反应器的优缺点：内部结构如何提高性能

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-30 DOI: 10.1016/j.bej.2024.109539

Carolin Bokelmann , Jason Bromley , Ralf Takors

{"title":"Pros and cons of airlift and bubble column bioreactors: How internals improve performance","authors":"Carolin Bokelmann , Jason Bromley , Ralf Takors","doi":"10.1016/j.bej.2024.109539","DOIUrl":"10.1016/j.bej.2024.109539","url":null,"abstract":"<div><div>Gas fermentation is a promising technology of high commercial interest, particularly for capturing CO<sub>2</sub> and CO from industrial off-gases to reduce greenhouse gas emissions and replace fossil fuels for bulk chemical production. Therefore, evaluating promising bioreactor settings <em>ab initio</em> is a crucial step. Whereas alternate configurations may be tested in laborious scale up studies, the procedure may be accelerated by <em>in silico</em> studies that accompany or even partially replace wet-lab work once the models are validated. In this context, the current study compares various pneumatically agitated reactor types – bubble column reactor (BCR), annulus- and center-rising internal-loop airlift reactor (AR-IL-ALR and CR-IL-ALR), and external-loop airlift reactor (EL-ALR) – to identify advantages and disadvantages for the given application based on computational fluid dynamics (CFD) models. Process performance is optimized by introducing internal structures to guide the flow. Despite a significant increase in the mass transfer coefficient (<span><math><mrow><msub><mrow><mi>k</mi></mrow><mrow><mi>L</mi></mrow></msub><mi>a</mi></mrow></math></span>) through internal modifications, the CR-IL-ALR still exhibited the poorest performance. The optimized AR-IL-ALR demonstrated good mixing and, after introducing an open-cone shaped internal in the head part and a conical bottom, superior mass transfer, achieving an enhancement over 10 % in the mass transfer coefficient to 315 1/h. This study thereby outlines the potential of internal structures for process improvement, as well as the value of <em>a priori in silico</em> design of reactor configurations.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109539"},"PeriodicalIF":3.7,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142577864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas9-mediated knockout of DYRK1B in triple-negative breast cancer cells: implications for cell proliferation, apoptosis, and therapeutic sensitivity CRISPR/Cas9 介导的三阴性乳腺癌细胞 DYRK1B 基因敲除：对细胞增殖、凋亡和治疗敏感性的影响

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-29 DOI: 10.1016/j.bej.2024.109553

Asrin Rashidi , Ernst-Martin Füchtbauer , Zakaria Vahabzadeh , Farzad Soleimani , Karim Rahimi , Bahram Nikkhoo , Shohreh Fakhari , Mohammad Bagher Khadem Erfan , Asaad Azarnezhad , Arash Pooladi , Fariborz Soheili , Fardin Fathi

{"title":"CRISPR/Cas9-mediated knockout of DYRK1B in triple-negative breast cancer cells: implications for cell proliferation, apoptosis, and therapeutic sensitivity","authors":"Asrin Rashidi , Ernst-Martin Füchtbauer , Zakaria Vahabzadeh , Farzad Soleimani , Karim Rahimi , Bahram Nikkhoo , Shohreh Fakhari , Mohammad Bagher Khadem Erfan , Asaad Azarnezhad , Arash Pooladi , Fariborz Soheili , Fardin Fathi","doi":"10.1016/j.bej.2024.109553","DOIUrl":"10.1016/j.bej.2024.109553","url":null,"abstract":"<div><div>Breast cancer is the most common cancer among women worldwide, with the triple-negative subtype (TNBC) having a poor prognosis and limited treatment options. DYRK1B is a dual-specificity kinase that regulates the cell cycle and quiescence. While its role in several cancers has been characterized, its role in TNBC remains unknown. In this study, we used CRISPR/Cas9 to delete DYRK1B in MDA-MB-231 cells, a model of TNBC and investigated its effects on cell proliferation, apoptosis, invasion, migration, angiogenesis, and response to Paclitaxel. The DYRK1B knockout (KO) was confirmed by PCR, Real-time qPCR, and Sanger sequencing. KO cells showed a significant reduction in cell proliferation, colony formation, invasion, and migration. Additionally, there were alterations in mRNA expression levels of several genes related to the cell cycle, angiogenesis, and cell motility, such as CCND1, MCM2, PCNA, CDKN1B, HIF1A, VEGFA, and WASF3, compared to MDA-MB-231 wild type (WT) cells. Immunocytochemistry results assessing Ki67 expression, a marker of cell proliferation, indicated that DYRK1B knockout cells had significantly lower Ki67 expression than WT cells. Furthermore, KO cells exhibited increased apoptosis and sensitivity to contact inhibition. Additionally, the IC<sub>50</sub> for Paclitaxel was significantly decreased in KO cells. These results suggest that DYRK1B plays an important role in the survival and invasion of TNBC cells and might be a potential candidate as a new therapeutic target for this disease.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109553"},"PeriodicalIF":3.7,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell Dome-based transfection array for non-adherent suspension cells 基于细胞穹顶的非粘附悬浮细胞转染阵列

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-29 DOI: 10.1016/j.bej.2024.109554

Ryotaro Kazama , Satoshi Fujita , Shinji Sakai

{"title":"Cell Dome-based transfection array for non-adherent suspension cells","authors":"Ryotaro Kazama , Satoshi Fujita , Shinji Sakai","doi":"10.1016/j.bej.2024.109554","DOIUrl":"10.1016/j.bej.2024.109554","url":null,"abstract":"<div><div>Cell-based microarrays are valuable tools for analyzing cellular functions. However, a significant limitation of conventional microarrays is their inapplicability to non-adherent cells. In this study, we investigated the potential of the ‘Cell Dome’ (diameter: 1 mm, height: approximately 300 µm) with a 90 µm-thick hydrogel shell as a gene transfection array for non-adherent cells in suspension. The human lymphoma cell line (K562 cells), used as a model for non-adherent cells, was transfected more efficiently by Lipofectamine/pDNA complexes on a composite hydrogel made of polyvinyl alcohol derivative (PVA-Ph) and chitosan derivative (chitosan-Ph) than hydrogels composed of an alginate derivative or PVA-Ph alone. Moreover, no significant adverse effects on the viability and proliferation of the enclosed cells were observed for Cell Dome with a PVA-Ph/chitosan-Ph composite hydrogel shell. Lipofectamine/pDNA complexes released from the bottom of Cell Domes could transfect the enclosed cells without leaking or contaminating adjacent Cell Domes. These results demonstrate the potential of Cell Domes with an appropriate hydrogel shell as transfection arrays for non-adherent cells in suspension, thereby expanding the range of applications of cell-based array technologies. This novel Cell-Dome transfection array would be a valuable tool for analyzing the cellular function of non-adherent cells in suspension and showcases the potential for providing important biomedical insights for future research and developments.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109554"},"PeriodicalIF":3.7,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Removal of lysozyme as protein waste using weak ion exchange nanofiber membrane in a batch system: Linear and nonlinear model analysis 在批处理系统中使用弱离子交换纳米纤维膜去除作为蛋白质废物的溶菌酶：线性和非线性模型分析

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-29 DOI: 10.1016/j.bej.2024.109550

Dinh Thi Hong Thanh , Nguyen The Duc Hanh , Bing-Lan Liu , Penjit Srinophakun , Chen-Yaw Chiu , Shen-Long Tsai , Kuei-Hsiang Chen , Yu-Kaung Chang

{"title":"Removal of lysozyme as protein waste using weak ion exchange nanofiber membrane in a batch system: Linear and nonlinear model analysis","authors":"Dinh Thi Hong Thanh , Nguyen The Duc Hanh , Bing-Lan Liu , Penjit Srinophakun , Chen-Yaw Chiu , Shen-Long Tsai , Kuei-Hsiang Chen , Yu-Kaung Chang","doi":"10.1016/j.bej.2024.109550","DOIUrl":"10.1016/j.bej.2024.109550","url":null,"abstract":"<div><div>This study investigates the removal of lysozyme, a common waste protein, using weak ion-exchange nanofiber membranes (P-COOH) to address challenges in protein waste management and wastewater treatment. Protein waste, prevalent in industrial and biological processes, contributes to environmental pollution and increases treatment complexity if not effectively managed. Conventional methods often face limitations in selectivity and efficiency, highlighting the need for innovative solutions. The P-COOH nanofiber membrane, with its high surface area and tailored functional groups, was engineered to optimize protein removal. The removal process was analyzed in a batch system at an optimal pH of 7. Various kinetic models, such as pseudo-first-order, pseudo-second-order, Avrami, and intra-particle diffusion, were applied to elucidate rate-controlling steps. In contrast, isotherm models, including Langmuir, Freundlich, and Temkin, characterized the equilibrium of the removal process. Complete elution of removed lysozyme was achieved using a 0.3 M NaCl solution, yielding an equilibrium binding capacity of 315.24 mg/g on the nanofiber membranes. These findings indicate that the Avrami nonlinear model best describes lysozyme removal's complex, multi-step kinetics. In contrast, the Langmuir linear model accurately fits the equilibrium data, suggesting monolayer removal on a homogeneous surface. The successful reusability of the membranes underscores their potential for sustainable lysozyme removal from wastewater, offering a viable solution for improved waste management in industrial applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109550"},"PeriodicalIF":3.7,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genosensor based on polypyrrole and dendrimer-coated gold nanoparticles for human papillomavirus detection 基于聚吡咯和树枝状聚合物包覆金纳米粒子的基因传感器，用于检测人类乳头瘤病毒

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-29 DOI: 10.1016/j.bej.2024.109551

Heloisa B. Dantas , Alberto G. Silva-Junior , Norma L.C.L. Silva , Abdelhamid Errachid , Maria D.L. Oliveira , Cesar A.S. Andrade

{"title":"Genosensor based on polypyrrole and dendrimer-coated gold nanoparticles for human papillomavirus detection","authors":"Heloisa B. Dantas , Alberto G. Silva-Junior , Norma L.C.L. Silva , Abdelhamid Errachid , Maria D.L. Oliveira , Cesar A.S. Andrade","doi":"10.1016/j.bej.2024.109551","DOIUrl":"10.1016/j.bej.2024.109551","url":null,"abstract":"<div><div>Human Papillomavirus (HPV) is an often asymptomatic widespread sexually transmitted infection responsible for causing various health issues. Low-risk HPV primarily causes genital warts. High-risk HPV types are associated with several cancers, including cervical, anal, and oropharyngeal cancers, posing significant health risks. In this work, we developed an electrochemical biosensor for the detection and differentiation of HPV genotypes based on electropolymerized polypyrrole (PPy) and PAMAM dendrimer-coated gold nanoparticles (PAMAM-AuNPs) for the immobilization of a DNA probe for detecting different HPV genotypes. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and atomic force microscopy (AFM) were used to characterize the biosensor. AFM analysis revealed varied topographic surfaces associated with the increased peaks concerning the biosensor and against patient samples. Electrochemical responses indicated that the genosensor could detect HPV using plasmid (HPV 6, 16, 18, 31, and 33) and cDNA samples (HPV 6, 18, and 31) from infected patients. Different electrochemical profiles were obtained between high-risk and low-risk genotypes. The sensor presented an excellent analytical performance, presenting a lower LOD of 0.04 pg.µL<sup>−1</sup> and 0.34 pg.µL<sup>−1</sup> for plasmidial and cDNA samples, respectively. Electrochemical analysis pointed out the ability of the developed genosensor platform to differentiate the HPV genotypes. The proposed biosensor is a promising tool for detecting and monitoring HPV and related diseases such as cervical cancer.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109551"},"PeriodicalIF":3.7,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142586056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-healing of concrete crack based on modified zeolite immobilizing microorganisms 基于固定微生物的改性沸石的混凝土裂缝自愈合技术

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-28 DOI: 10.1016/j.bej.2024.109541

Nansha Ye , Zhen Liu , Peng Wang , Yongshuai Sun , Xiangli He

{"title":"Self-healing of concrete crack based on modified zeolite immobilizing microorganisms","authors":"Nansha Ye , Zhen Liu , Peng Wang , Yongshuai Sun , Xiangli He","doi":"10.1016/j.bej.2024.109541","DOIUrl":"10.1016/j.bej.2024.109541","url":null,"abstract":"<div><div>Selecting the appropriate carrier to safeguard the microorganisms and enhance their viability within the matrix while implementing self-healing in concrete by microbial induced calcium carbonate precipitation technology is challenging. In this study, modified zeolite was used as the microbial carrier for concrete. Varying concentrations of acid and alkali reagents were used to impregnate natural zeolite, and the optimal modification reagent was identified. The mechanical compatibility of zeolite and concrete was analyzed. The mineralization efficacy of microorganisms was assessed by analyzing the self-healing indices of concrete crack specimens, the optimal dosage of modified zeolite was determined. And the mechanism of this system was explored. The results indicate that when the bacterial solution concentration is 3×10<sup>7</sup> cfu/mL, incorporating modified zeolite (particle size: 1.0–2.0 mm) with a 0.4 mol/L HCl solution yields the optimal microbial immobilization and mineralization effects. Also, with equivalent zeolite dosage, modified zeolite self-healing concrete can enhance compressive strength recovery by 8–22 % and reduce water absorption by 11–20 %, making it capable of completely repairing wider cracks. Concrete specimens with 30 % and 40 % modified zeolite content demonstrated excellent performance, with the 40 % dosage exhibiting the highest self-healing capability. At the 40 % modified zeolite dosage, concrete achieved a remarkable 86.8 % compressive strength recovery at 28 days and a 5.4 % water absorption rate, effectively repairing cracks up to 0.5 mm width.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109541"},"PeriodicalIF":3.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142577865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CFD-guided scaling of Pseudomonas putida fermentation 以 CFD 为指导的假单胞菌发酵规模化

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-28 DOI: 10.1016/j.bej.2024.109549

Maryam Jamshidzadeh , Antonia Ursula Griesz , Jesper Wang Jensen , Ulrich Krühne , John M. Woodley , Krist V. Gernaey , Pablo Ivan Nikel , Helena Junicke

{"title":"CFD-guided scaling of Pseudomonas putida fermentation","authors":"Maryam Jamshidzadeh , Antonia Ursula Griesz , Jesper Wang Jensen , Ulrich Krühne , John M. Woodley , Krist V. Gernaey , Pablo Ivan Nikel , Helena Junicke","doi":"10.1016/j.bej.2024.109549","DOIUrl":"10.1016/j.bej.2024.109549","url":null,"abstract":"<div><div>This study evaluated the scale-up of <em>Pseudomonas putida</em> fed-batch fermentation from a 2 L benchtop-scale stirred bioreactor to a 200 L pilot-scale tank by using a validated computational fluid dynamic (CFD) model. One of the major concerns in this fermentation process is the potential reduction in mixing quality with increasing scale, leading to lower yield or product quality. For a low-risk scale-up, a multiphase Euler-Euler CFD model was developed that simulated the hydrodynamics of the fed-batch system at various filling volumes, representing different stages of the fermentation process. The model was validated using experimental data of mixing time and mass transfer coefficient. The hydrodynamic model was then coupled with a Monod kinetic model of <em>P. putida</em> ‘s fermentation. Response surface methodology was used to generate a performance map of the pilot bioreactor at various aeration, agitation, and bioreactor filling volumes. The study considered different established scale-up approaches, such as constant tip speed and aeration rate across scales, constant <span><math><mrow><msub><mrow><mi>k</mi></mrow><mrow><mi>L</mi></mrow></msub><mi>a</mi></mrow></math></span>, as well as constant power to unit of liquid volume (P/V). The performance of the bioreactor was assessed, and the optimum operating ranges of the input parameters were obtained at different stages of the fermentation. Using performance map the possibility of substrate and oxygen gradient formation, and the gradient severity inside the pilot bioreactor at different working volumes were evaluated.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109549"},"PeriodicalIF":3.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142593620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined activated sludge and sand filtration for purification of UASB effluent with high suspended solids from water hyacinth juice 将活性污泥和砂滤结合起来，净化含有高悬浮固体的布袋莲汁污水

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-24 DOI: 10.1016/j.bej.2024.109540

Akinori Fujita , Mutsumi Sekine , Masatoshi Kishi , Tatsuki Toda

{"title":"Combined activated sludge and sand filtration for purification of UASB effluent with high suspended solids from water hyacinth juice","authors":"Akinori Fujita , Mutsumi Sekine , Masatoshi Kishi , Tatsuki Toda","doi":"10.1016/j.bej.2024.109540","DOIUrl":"10.1016/j.bej.2024.109540","url":null,"abstract":"<div><div>Rapid biogasification of water hyacinth, a globally overgrown species, using compression and up-flow anaerobic sludge blanket (UASB) treatment of the juice has recently been proposed. This study aimed to establish a post-purification system for UASB-treated juice to effectively utilize the remaining nutrients for valuable products, such as vegetables and microalgae. To easily remove hard-to-degrade suspended solids (SS) and organic matter from UASB-treated juice, the activated sludge (AS) and sand filtration processes—conventional biological treatments—were introduced, with a focus on their physical treatment properties. Over 76 % of SS and 43 % of the organic carbon were removed from UASB-treated juice during the process. The hydraulic retention time of the AS was reduced to one day, indicating the potential of minimizing the facility size. The light transmittance, which is crucial for microalgae mediums, improved sevenfold. Even after most of the SS was removed by the AS, the sand filtration further increased the light transmittance by 1.4 times. The AS effectively removed most of the SS and organic matter, whereas sand filtration enhanced the treatment stability and further improved the light transmittance. NH<sub>4</sub><sup>+</sup> was mostly oxidized to NO<sub>3</sub><sup>-</sup>, which is less toxic to plants. The total inorganic nitrogen content remained high after treatment, suggesting that UASB effluents from water hyacinth can be used as a nutrient source for hydroponics and microalgae cultivation in developing countries.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109540"},"PeriodicalIF":3.7,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142593621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0