Biochemical Engineering Journal最新文献_第4页

Simultaneous enzymatic hydrolysis and bioconversion of deacetylated and disk refined sugarcane bagasse to single-cell protein: An experimental investigation and techno-economic analysis

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-24 DOI: 10.1016/j.bej.2025.109691

Veronica Lourens, Catharine E. Bosman , Abdul M. Petersen , Gerhardt Coetzee, Johann F. Görgens , Eugéne van Rensburg

{"title":"Simultaneous enzymatic hydrolysis and bioconversion of deacetylated and disk refined sugarcane bagasse to single-cell protein: An experimental investigation and techno-economic analysis","authors":"Veronica Lourens, Catharine E. Bosman , Abdul M. Petersen , Gerhardt Coetzee, Johann F. Görgens , Eugéne van Rensburg","doi":"10.1016/j.bej.2025.109691","DOIUrl":"10.1016/j.bej.2025.109691","url":null,"abstract":"<div><div>Rising animal feed costs are driving up animal agriculture and food prices. While single-cell protein (SCP) production from lignocellulosic waste offers a solution, pretreatment of these feedstocks is required. In this study, the SCP yield from sugarcane bagasse pretreated via deacetylation in combination with disk refining (DDR), prior to simultaneous enzymatic hydrolysis and fermentation using <em>P. ostreatus</em> and <em>F. venenatum</em> compared to <em>S. cerevisiae</em>, was investigated. A biorefinery was simulated to investigate the economic feasibility of animal feed enhanced with SCP, using a discounted cashflow analysis. Deacetylation and DDR were effective pretreatment approaches, improving enzymatic digestibility from 9.10 g sugar/100 g dry matter (DM) to 75.75 and 80.14 g sugar/100 g DM, respectively, which was comparable to steam-explosion pretreatment. Pretreated bagasse was successfully converted to SCP through simultaneous enzymatic hydrolysis and bioconversion. However, DDR was preferred for bioconversions using <em>P. ostreatus</em> and <em>F. venenatum</em>, achieving SCP yields of 4.24 ± 0.17 and 3.47 ± 0.33 g protein/100 g DM, respectively. In the ranges investigated, solids loading had a more pronounced effect on the SCP yield than enzyme dosage, irrespective of the microorganism used. However, the minimum selling price of animal feed enhanced with SCP (0.69 US$/kg) remains higher than the market price of animal feeds like soybean meal (0.40 US$/kg). The technology shows promise but can be further optimised.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"218 ","pages":"Article 109691"},"PeriodicalIF":3.7,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergy between horseradish peroxidase HRP and edible gelatin: A simple way to remove phenols and dyes simultaneously from wastewater

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-23 DOI: 10.1016/j.bej.2025.109692

Jianling Feng , Huadeng Li , Ke Zheng , Yanbin Zeng , Guanqun Xie , Xiaoxia Wang

{"title":"Synergy between horseradish peroxidase HRP and edible gelatin: A simple way to remove phenols and dyes simultaneously from wastewater","authors":"Jianling Feng , Huadeng Li , Ke Zheng , Yanbin Zeng , Guanqun Xie , Xiaoxia Wang","doi":"10.1016/j.bej.2025.109692","DOIUrl":"10.1016/j.bej.2025.109692","url":null,"abstract":"<div><div>Wastewater containing phenols or dyes is quite common. Given the potential harmful effects of such wastewater on both the environment and human health, there is a continuing demand for efficient, green, and cost-effective methods to remove these pollutants. In this study, edible gelatin was employed as an additive in conjunction with horseradish peroxidase (HRP) to purify wastewater containing phenols and dyes. Under optimized conditions, the combination of HRP and gelatin effectively purified various types of wastewater containing different phenols, achieving removal rates of up to 90 %. Furthermore, this strategy was successfully applied to the degradation of mixed wastewater containing dyes (methyl orange, methylene blue or/and rhodamine B) in the presence of phenol. High removal rates for both phenol and the dyes were also observed and the TOC removal rate reached as high as 94 %. Finally, a removal mechanism involving the HRP/gelatin system was proposed based on UV-Vis detection, particle size analysis, LC-MS, and TOC results. This green and efficient strategy for the removal of phenols and dyes from wastewater represents a valuable contribution to sustainable environmental remediation.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"218 ","pages":"Article 109692"},"PeriodicalIF":3.7,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143508784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete scheme for biosuccinic acid production from candy-factory waste with simultaneous fermentation-electrochemical product recovery, and purification

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-21 DOI: 10.1016/j.bej.2025.109687

Ioannis Zacharopoulos, Antonio Grimalt-Alemany, Christoforos Tsoumanis, Irini Angelidaki

{"title":"Complete scheme for biosuccinic acid production from candy-factory waste with simultaneous fermentation-electrochemical product recovery, and purification","authors":"Ioannis Zacharopoulos, Antonio Grimalt-Alemany, Christoforos Tsoumanis, Irini Angelidaki","doi":"10.1016/j.bej.2025.109687","DOIUrl":"10.1016/j.bej.2025.109687","url":null,"abstract":"<div><div>Succinic acid is a high-value platform chemical with a wide range of industrial applications, making its sustainable production an area of increasing interest. However, a key challenge preventing biologically produced succinic acid from becoming economically competitive with petrochemical-based production lies in the inefficiencies associated with both upstream and downstream processing. In this study, we present a comprehensive platform for the biological production of succinic acid, using industrial waste from candy production as a substrate. This approach integrates upstream and downstream processes through the use of an in-situ electrochemical succinic acid recovery system, significantly simplifying the overall production workflow. As a result, the downstream process was reduced to just five steps, compared to the more complex nine-step processes described in existing literature. The proposed method demonstrated high separation yields of 86 % and a purity level of 92 %, showcasing its efficiency. Furthermore, the in-situ recovery strategy led to a 15 % increase in succinic acid yield, a 10.2 % improvement in overall productivity, and a 13.5 % increase in substrate consumption when compared to traditional batch fermentation processes. These results highlight the potential of this integrated platform to enhance the economic feasibility of biologically produced succinic acid on an industrial scale.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"218 ","pages":"Article 109687"},"PeriodicalIF":3.7,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143480488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of (R)-acetoin by targeted immobilization of acetolactate synthase and acetolactate decarboxylase via the spy/snoop system

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-19 DOI: 10.1016/j.bej.2025.109671

Kexin Shao , Yadong Wang , Peng Chen , Fenghuan Wang

{"title":"Synthesis of (R)-acetoin by targeted immobilization of acetolactate synthase and acetolactate decarboxylase via the spy/snoop system","authors":"Kexin Shao , Yadong Wang , Peng Chen , Fenghuan Wang","doi":"10.1016/j.bej.2025.109671","DOIUrl":"10.1016/j.bej.2025.109671","url":null,"abstract":"<div><div>Acetoin (3-hydroxy-2-butanone) is an important four-carbon platform compound and an intermediate for several high-value-added pharmaceutical compounds. In this study, Catcher-Tag tagged proteins were used for targeted immobilization of acetolactate synthase (ALS) and acetolactate decarboxylase (ALDC) for catalyzing the synthesis of Acetoin from sodium pyruvate in vitro. The study discussed the enzymatic properties of the immobilized enzyme LX@ALS-SpyTag 和 LX@ALDC-SnoopTag, as well as the optimal catalytic conditions and reusability of the immobilized dual enzyme in vitro catalytic system. The immobilized enzyme exhibited higher relative activity across a broader range of temperature and pH intervals than the free enzyme. The in vitro catalytic synthesis of (R)-acetoin from sodium pyruvate using an immobilized dual enzyme system resulted in 99.95 % of the theoretical conversion of (R)-acetoin at 45°C, pH 7.0, and 0.2 M substrate concentration. In addition, the immobilized dual enzyme system had better operational stability than the free enzyme, retaining 69.20 % of the initial total catalytic activity after 5 cycles. In conclusion, this study demonstrated a promising and convenient Catcher-Tag tagged protein immobilization strategy that enables rapid targeted immobilization of tagged proteins directly from crude enzyme solution, providing a novel approach for the construction of in vitro immobilized multi-enzyme complexes.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109671"},"PeriodicalIF":3.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering Pichia pastoris for high-level biosynthesis of squalene

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-19 DOI: 10.1016/j.bej.2025.109677

Xinying Zhang , Yuxin Yang , Shuting Chen , Ying Lin , Yanxuan Wu , Shuli Liang

{"title":"Engineering Pichia pastoris for high-level biosynthesis of squalene","authors":"Xinying Zhang , Yuxin Yang , Shuting Chen , Ying Lin , Yanxuan Wu , Shuli Liang","doi":"10.1016/j.bej.2025.109677","DOIUrl":"10.1016/j.bej.2025.109677","url":null,"abstract":"<div><div>High-value-added chemicals and advanced biofuels can be feasibly produced from renewable feedstocks through microbial metabolic engineering. Methanol is produced with high quantities from CO<sub>2</sub> using green energy sources such as solar energy, thereby making it an ideal feedstock. However, little has been documented on the microbial production of terpenoids utilising methanol. This study aimed to engineer <em>Pichia pastoris</em> to over-produce triterpene squalene from methanol by optimising and reconstructing the utilisation pathways of mevalonate and methanol. In addition, we explored the exocytosis of squalene and promoted squalene secretion successfully. Furthermore, we identified a key bottleneck of squalene synthesis in <em>P. pastoris</em> through transcriptomic analysis. The final engineered strain produced 20.80 ± 0.02 g/L squalene during fed-batch fermentation, which is the highest squalene production using organic one-carbon as carbon source reported so far. Our findings set the stage for applying <em>P. pastoris</em> as an organic one-carbon platform for producing biochemicals and biofuels.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109677"},"PeriodicalIF":3.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel magnetic fluorescent nanoprobe for simultaneous separation and identification of microRNA199a in breast cancer 一种新型磁性荧光纳米探针，用于同时分离和鉴定乳腺癌中的 microRNA199a

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-19 DOI: 10.1016/j.bej.2025.109676

Delaram Enteshari , Razieh Ghasemi , Flora Forouzesh

{"title":"A novel magnetic fluorescent nanoprobe for simultaneous separation and identification of microRNA199a in breast cancer","authors":"Delaram Enteshari , Razieh Ghasemi , Flora Forouzesh","doi":"10.1016/j.bej.2025.109676","DOIUrl":"10.1016/j.bej.2025.109676","url":null,"abstract":"<div><h3>Background</h3><div>Rapid and early diagnosis of the specific biomarkers and symptoms can increase the efficiency of treatment and reduce the mortality rate of breast cancer as the second cause of cancer death among women worldwide. MicroRNA199 (miR199) as a member of the most important microRNAs families is one of these circulating biomarkers that its dysregulation is associated with the development and progression or suppression of variety of cancers.</div></div><div><h3>Method</h3><div>In this regard, a magnetic fluorescent nanoprobe was designed and fabricated for separation and identification of microRNA199 (miR199) biomarker in breast cancer. Therefore, the surfaces of synthesized silica-coated Fe<sub>3</sub>O<sub>4</sub> and CdTe quantum dots were functionalized with two specific probes for miR199 and then characterization techniques were performed to confirm the nanoprobes synthesis steps. The performance of magnetic fluorescent nanoprobes was analyzed by hybridization process of nanoprobes and miR199 sequences. Subsequently, nanoprobes hybridization steps were performed by the extracted RNAs from 10 samples from women with breast cancer and the expression of miR199a in clinical samples was examined by Real-Time PCR.</div></div><div><h3>Results</h3><div>The results showed that the limit of detection is as low as 0.48 pM and separation and identification can be performed in less than an hour with high specificity.</div></div><div><h3>Conclusion</h3><div>The synthesized nanoprobes were able to rapid isolation and identification of the MicroRNA199 with high specificity and sensitivity.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"218 ","pages":"Article 109676"},"PeriodicalIF":3.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scaling iPSC production in different 3D platforms in suspension culture for their use in allogeneic regenerative therapies

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-19 DOI: 10.1016/j.bej.2025.109672

Danton Freire-Flores , Nyna Kawles , Pablo Caviedes , Barbara A. Andrews , Juan A. Asenjo

{"title":"Scaling iPSC production in different 3D platforms in suspension culture for their use in allogeneic regenerative therapies","authors":"Danton Freire-Flores , Nyna Kawles , Pablo Caviedes , Barbara A. Andrews , Juan A. Asenjo","doi":"10.1016/j.bej.2025.109672","DOIUrl":"10.1016/j.bej.2025.109672","url":null,"abstract":"<div><div>Human induced pluripotent stem cells (iPSCs) exhibit significant potential for regenerative medical treatments due to their pluripotency and proliferation capacity. However, the large-scale production required for allogeneic therapies necessitates efficient and scalable culture systems. Conventional 2D culture techniques are inadequate for this, driving interest for innovative bioreactor-based expansion techniques such as microcarrier-based or aggregate cultures. In this study, we compare iPSC expansion and quality in microcarrier and aggregate cultures in spinner flasks, aiming to ascertain which 3D growth configuration yields superior cell mass while maintaining cellular quality. We demonstrated comparable expansion of iPSCs on Cytodex 1 and Cultisphere G microcarriers, as well as in aggregates by day 6 in culture, achieving a final cell density of <span><math><mrow><mn>2</mn><mo>.</mo><mn>6</mn><mo>*</mo><msup><mrow><mn>10</mn></mrow><mrow><mn>6</mn></mrow></msup><mspace></mspace></mrow></math></span>cells/ml and <span><math><mrow><mn>5</mn><mo>.</mo><mn>67</mn><mo>*</mo><msup><mrow><mn>10</mn></mrow><mrow><mn>6</mn></mrow></msup></mrow></math></span> cells/ml on microcarriers respectively, and <span><math><mrow><mn>9</mn><mo>.</mo><mn>76</mn><mo>*</mo><msup><mrow><mn>10</mn></mrow><mrow><mn>6</mn></mrow></msup></mrow></math></span> cells/ml in aggregates, yielding 4- and 9-fold expansion on microcarriers and 15-fold in aggregates. The efficiency in the recovery of iPSCs as single cells was over 91.5 % for all cultures. iPSCs cultured on microcarriers and as aggregates exhibit OCT3/4, SSEA4, TRA-1–60, and SOX2 pluripotency marker expression, as well as retention of differentiation potential into three germinal layers. This work supports the utilization of microcarriers and aggregates as efficient 3D expansion platforms for suspension cultures of iPSCs.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109672"},"PeriodicalIF":3.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using metagenomics to reveal the effects of zero-valent iron with different sizes on the mesophilic and thermophilic anaerobic digestion of sludge

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-18 DOI: 10.1016/j.bej.2025.109688

Jianglin Chen , Wenqian Li , Lina Pang , Efthalia Chatzisymeon , Yuanyuan Lu , Ping Yang

{"title":"Using metagenomics to reveal the effects of zero-valent iron with different sizes on the mesophilic and thermophilic anaerobic digestion of sludge","authors":"Jianglin Chen , Wenqian Li , Lina Pang , Efthalia Chatzisymeon , Yuanyuan Lu , Ping Yang","doi":"10.1016/j.bej.2025.109688","DOIUrl":"10.1016/j.bej.2025.109688","url":null,"abstract":"<div><div>This work investigated the mechanisms of anaerobic digestion (AD) of sludge to the addition of zero-valent iron (ZVI) at three particle sizes under mesophilic (MAD) and thermophilic (TAD) temperatures. Results showed that nano-ZVI, micron-ZVI, and iron scrap all enhanced the cumulative methane production in two AD processes. Besides, the highest cumulative methane yield (83.92 mL/g VS) was achieved in MAD with nano-ZVI, which possibly stimulated the hydrolysis and acidification processes in MAD. Moreover, metagenomic results revealed that the more abundant predominant methanogenic pathways and functional microbes like genera <em>Candidatus_Microthrix</em> and <em>Methanothrix</em>, and the enriched enzymes associated with hydrotrophic and methyl methanogenesis pathways, might be the underlying reasons for the best performance of MAD with nano-ZVI. This study provides knowledge underpinning the mechanisms for the deployment of ZVI in solid waste anaerobic digestion to effectively manage and treat waste and increase biomethane production.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109688"},"PeriodicalIF":3.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient production of 9α-hydroxy-steroid from phytosterols in Mycobacterium fortuitum ATCC 6842 by modifying multiple genes

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-18 DOI: 10.1016/j.bej.2025.109689

Ruijie Zhang , Wen Sun , Suwan Han , Xiaoxuan Sun , Xinghui Zhai , Beiru He , Xianfeng Zhu , Baoguo Zhang

{"title":"Efficient production of 9α-hydroxy-steroid from phytosterols in Mycobacterium fortuitum ATCC 6842 by modifying multiple genes","authors":"Ruijie Zhang , Wen Sun , Suwan Han , Xiaoxuan Sun , Xinghui Zhai , Beiru He , Xianfeng Zhu , Baoguo Zhang","doi":"10.1016/j.bej.2025.109689","DOIUrl":"10.1016/j.bej.2025.109689","url":null,"abstract":"<div><div>The steroid drug industry is increasingly utilizing microbial biotransformation, employing genetically modified mycobacteria to convert phytosterols into steroid intermediates, with an emphasis on improving yield and purity. This study enhances the production of 9α-hydroxy-4-androstene-3,17-dione (9-OH-AD), a vital C19 steroid intermediate for glucocorticoid synthesis, by genetically modifying <em>Mycobacterium fortuitum</em> ATCC 6842. The study involved the targeted disruption of five 3-ketosteroid-Δ<sup>1</sup>-dehydrogenase (<em>kstD</em>) genes to prevent Δ<sup>1</sup>-dehydrogenation. The purity of 9-OH-AD is initially low at 81.85 % due to two main by-products: 9,22-dihydroxy-23,24-bisnorchol-4-ene-3-one (9-OH-HP) and 9,24-dihydroxychol-4-en-3-one (9,24-DHC). To address this, the steroid aldolase (<em>sal</em>) gene was deleted to block the C22 metabolic pathway, which completely eliminated 9-OH-HP and increased the purity of 9-OH-AD to 88.19 %. To further reduce 9,24-DHC levels, acyl-CoA dehydrogenases ChsE1 and ChsE2 were overexpressed. The resulting strain, MFKS_<em>chsE1</em>-<em>chsE2</em>, achieved a high purity of 9-OH-AD at 94.96 %, with a molar yield of 87.17 % from 10 g/L phytosterols, and converted up to 30 g/L of phytosterols into 15.91 g/L of 9-OH-AD. This method effectively enhances both the production and purity of this important steroid intermediate.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"218 ","pages":"Article 109689"},"PeriodicalIF":3.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143464751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterologous expression of algal fucosyltransferases and sulfotransferases in Escherichia coli

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-02-18 DOI: 10.1016/j.bej.2025.109690

Ahmed Zayed , Benjamin Ledermann , Thomas Fischöder , Lothar Elling , Nicole Frankenberg-Dinkel , Roland Ulber

{"title":"Heterologous expression of algal fucosyltransferases and sulfotransferases in Escherichia coli","authors":"Ahmed Zayed , Benjamin Ledermann , Thomas Fischöder , Lothar Elling , Nicole Frankenberg-Dinkel , Roland Ulber","doi":"10.1016/j.bej.2025.109690","DOIUrl":"10.1016/j.bej.2025.109690","url":null,"abstract":"<div><div>Alternative to classical production of fucoidans, enzymatic synthesis has been proposed especially following genomic and bioinformatic investigations in <em>Ectocarpus siliculosus</em> and <em>Saccharina</em> sp. The present research aimed to assess the activity of four putative algal enzymes, including fucosyltransferases (FucTs_21 and FucTs_50) and carbohydrate sulfotransferases (STs_32 and STs_283) expressed in <em>E. coli</em> BL21 (DE3) for future application in lab-synthesized fucoidans. A specific activity of 0.47 pmol.min<sup>−1</sup>.µg<sup>−1</sup> was observed for FucTs_50 in the Glycosyltransferase activity assay, while multiplexed capillary electrophoresis (MP-CE) revealed that almost 32 % of GDP-L-fucose was affected. Additionally, the Universal Sulfotransferase Activity Kit was used to evaluate STs, and the results indicated that STs_283 and STs_32 exhibited specific activities at 2.29 and 2.38 pmol.min<sup>−1</sup>.µg<sup>−1</sup>, respectively. The results indicated correctly folded active enzymes with binding sites specific to their corresponding substrates.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"218 ","pages":"Article 109690"},"PeriodicalIF":3.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143489027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0