Biochemical Engineering Journal最新文献_第5页

Comparative techno-economic assessment of multi-feedstock to multi-product integrated lignocellulosic biorefinery 多原料与多产品一体化木质纤维素生物炼制的技术经济比较评价

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-08-08 DOI: 10.1016/j.bej.2025.109892

Georgeio Semaan , Abdullah Bilal Öztürk , Gopalakrishnan Kumar

{"title":"Comparative techno-economic assessment of multi-feedstock to multi-product integrated lignocellulosic biorefinery","authors":"Georgeio Semaan , Abdullah Bilal Öztürk , Gopalakrishnan Kumar","doi":"10.1016/j.bej.2025.109892","DOIUrl":"10.1016/j.bej.2025.109892","url":null,"abstract":"<div><div>A techno‑economic assessment was performed for a multi‑feedstock lignocellulosic biorefinery that converts Norwegian tomato and cucumber crop residues, spent coffee grounds, and brewers’ spent grain into value‑added chemicals. Scenario 1 integrates dilute oxalic‑acid hydrolysis (DAH) with oxalic‑acid‑assisted ethanol‑organosolv delignification (ORG) and co‑produces ethanol, lactic acid, ethanol‑organosolv lignin, furfural, 5‑hydroxymethylfurfural, and electricity. Scenario 2 omits the lactic‑acid train, whereas Scenario 3 employs DAH, only without the ORG or lactic acid sections. Mass and energy balance simulations informed discounted cash flow models for feedstock capacities of 25 and 250 kt/yr. At 25 kt/yr, all scenarios were unprofitable. Scaling to 250 kt/yr improved outcomes for Scenarios 1 and 3, yielding 11.5–12.6 % internal rate of return (IRR), US$ 183.7–185MM net present value (NPV). ORG inclusion reduced overall returns due to elevated capital and operating costs. Integrated operation produced negative net unit production costs, indicating internal cost cross-subsidization among co-products. Sensitivity analysis identified revenue and operating expenditures as primary determinants of NPV and IRR. Monte Carlo analysis estimated a 93.7 % probability of profitability in all simulated outcomes. The findings underscore the importance of scaling up and diversifying product portfolio in future biorefinery deployments.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"224 ","pages":"Article 109892"},"PeriodicalIF":3.7,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144827766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maximizing yield, purity and throughput of M13 bacteriophage bioprocessing 最大限度地提高M13噬菌体生物处理的产量、纯度和吞吐量

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-08-06 DOI: 10.1016/j.bej.2025.109890

Daniel Modafferi, Julia Maria de Medeiros Dantas, Noémie-Manuelle Dorval Courchesne

{"title":"Maximizing yield, purity and throughput of M13 bacteriophage bioprocessing","authors":"Daniel Modafferi, Julia Maria de Medeiros Dantas, Noémie-Manuelle Dorval Courchesne","doi":"10.1016/j.bej.2025.109890","DOIUrl":"10.1016/j.bej.2025.109890","url":null,"abstract":"<div><div>The filamentous M13 bacteriophage has been used extensively as a building block for biomaterials fabrication due to its self-assembled structure and bulk assembling properties. With its genetic code encapsulated in its protein capsid, the M13 phage can be used in phage display or directed evolution mutagenesis for the creation of large libraries. Applying phage display to materials fabrication draws out bioprocessing challenges regarding high yields and high purity. Additionally, phage display introduces the need for high throughput production to parse mutant libraries. Here, we develop an optimized, high throughput process for upstream and downstream M13 phage production. We identify an optimal medium containing 17 g/L of both tryptone and yeast extract, maximizing phage production using standard polyethylene glycol/sodium chloride precipitation. Next, we add a centrifuge filtration step, which removes detectable traces of sodium ions and significantly lowers polyethylene glycol levels. The higher yields grown in the optimal medium remediate the loss of phages from added purification steps. We also applied this combined process to 96-well plates, recovering titers of purified phages proportional to those obtained with larger volumes. The method that we present here could allow for automatable, scalable M13 phage production for applications in genetically engineered biologically derived materials.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"224 ","pages":"Article 109890"},"PeriodicalIF":3.7,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144827036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotemplated self-assembly of ZIF-8 on Vischeria sp. WL1 for efficient uranium removal ZIF-8在Vischeria sp. WL1上的生物模板自组装高效除铀

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-08-05 DOI: 10.1016/j.bej.2025.109884

Changhuang Cao, Chaoli Shao, Wei Zhang, Yuantao Chen, Shutian Liu, Ting Zhang

{"title":"Biotemplated self-assembly of ZIF-8 on Vischeria sp. WL1 for efficient uranium removal","authors":"Changhuang Cao, Chaoli Shao, Wei Zhang, Yuantao Chen, Shutian Liu, Ting Zhang","doi":"10.1016/j.bej.2025.109884","DOIUrl":"10.1016/j.bej.2025.109884","url":null,"abstract":"<div><div>Uranium contamination in water poses serious environmental and health risks, necessitating efficient removal technologies. In this study, a novel bio-template strategy was developed to fabricate a high-performance uranium adsorbent. Nitrogen-starved <em>Vischeria</em> sp. WL1 microalgae with reinforced cell walls were used as a support for the in situ growth of ZIF-8, forming a robust composite (V.sp.WL1@ZIF-8). The resulting material exhibited outstanding U(VI) adsorption capacity, with a Langmuir maximum uptake of about 581 mg/g at 298 K. Adsorption kinetics are rapid, reaching equilibrium within 90 min, and fit well with the pseudo-second-order model, suggesting a chemisorption mechanism dominated by surface complexation, with intraparticle diffusion also contributing to the overall rate. Thermodynamic parameters (ΔG < 0, ΔH > 0) confirmed the adsorption process is spontaneous and endothermic, with higher temperatures enhancing capacity. The composite also shows good selectivity in the presence of competing ions (tolerating common cations) and maintains approximately 81 % of its initial capacity after six adsorption–desorption cycles, indicating excellent reusability. These results highlight the performance benefits due to the algae template and ZIF-8 synergy. This work uniquely utilizes nitrogen-starved Vischeria sp. as a robust bio-template for in situ ZIF-8 growth, introducing a novel strategy to enhance MOF–algae integration for uranium remediation. In conclusion, the V.sp.WL1@ZIF-8 composite is a promising and effective material for U(VI) removal from wastewater, offering improved capacity, stability, and potential for practical environmental remediation applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"224 ","pages":"Article 109884"},"PeriodicalIF":3.7,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144842035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vitamin supplementation for enhanced production of therapeutic nanobody in E.coli 补充维生素促进大肠杆菌治疗性纳米体的产生

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-29 DOI: 10.1016/j.bej.2025.109887

Richa Katiyar , Neha Gindi , Ishika Mittal , Claire Komives , Anurag S. Rathore

{"title":"Vitamin supplementation for enhanced production of therapeutic nanobody in E.coli","authors":"Richa Katiyar , Neha Gindi , Ishika Mittal , Claire Komives , Anurag S. Rathore","doi":"10.1016/j.bej.2025.109887","DOIUrl":"10.1016/j.bej.2025.109887","url":null,"abstract":"<div><div>As low yields of nanobodies in <em>E.coli.</em> periplasm is a major concern for their large-scale production, this study explores the use of vitamins for enhanced production of a recombinant periplasmic nanobody product. Nanobody producing <em>E.coli</em> cells were cultivated in the modified Terrific-Broth supplemented with three different vitamins (biotin denoted as B, cyanocobalamin as C, and D-calcium pantothenate as P). Multiple combinations (B+P, B+C, P + C, and B+P + C) were examined, with B+P offering the highest specific growth rate, total biomass productivity, product accumulation and product expression. Densitometry analysis revealed 129.17 ± 1.1 mg/L nanobodies in case of B+P supplementation (24 % higher than control). B+P supplementation also offered 21 % better fermentation yield than control (as per RP-HPLC). The yield of purified nanobody product in case of B+P supplementation was at 35.0 ± 2.1 mg/L with purity levels of ≥ 80 %. Intact mass and peptide mapping analysis of purified nanobody revealed molecular weight of 15.2 kDa and 91 % sequence coverage with the theoretical nanobody sequence respectively. Spent media analysis revealed production of low acetate in case of B+P supplementation of media when compared to other conditions examined. Hence, the B+P supplementation could be used to produce improved amount of periplasmic nanobody in future.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109887"},"PeriodicalIF":3.7,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144739424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Life cycle assessment of anaerobic digestion system under ammonia inhibition: Evaluation of exogenous media addition 氨抑制下厌氧消化系统的生命周期评价：外源培养基添加的评价

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-28 DOI: 10.1016/j.bej.2025.109886

Meiying Ye , Shuo Gao , Panpan Wang , Yufang Wei , Jiaqi Hou , Mingxiao Li , Yanping Liu , Ziyi Yang

{"title":"Life cycle assessment of anaerobic digestion system under ammonia inhibition: Evaluation of exogenous media addition","authors":"Meiying Ye , Shuo Gao , Panpan Wang , Yufang Wei , Jiaqi Hou , Mingxiao Li , Yanping Liu , Ziyi Yang","doi":"10.1016/j.bej.2025.109886","DOIUrl":"10.1016/j.bej.2025.109886","url":null,"abstract":"<div><div>Exogenous media addition in the ammonia-inhibited anaerobic digestion (AD) is considered as an efficient approach. Comprehensively evaluation of the environmental impact of adding exogenous media can provide the support on application. Under ammonia inhibition, life cycle assessment (LCA) was used to compare the AD system (Scenario 1) and the AD system with the addition of magnetite powder and polyurethane filler (Scenario 2). In the midpoint environmental impact assessment, among the 18 categories of environmental impacts, Scenario 2 performed the weak negative impact on the environment compared to the non-added scenario. Ionizing radiation and freshwater eutrophication in Scenario 2 were less than 50 % of those in Scenario 1. In the endpoint environmental impact assessment, the negative impacts on human health, ecosystems, and resource of Scenario 2 were lower than 82 % of those in the non-additive scenario. Monte Carlo simulation was used to carry out uncertain analysis and calculation, which effectively reduced the deviation brought by inventory analysis and improved the reliability of the analysis. The sensitivity analysis results showed that power input and power sources significantly affect environmental impacts. Reducing power input and using renewable or clean energy could decrease pollution levels. The scenario of adding exogenous media showed good environmental benefits, and subsequent optimization could focus on the aspects of power source (such as using green electricity) and power efficiency.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109886"},"PeriodicalIF":3.7,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144714482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sustainable microalgae harvesting method via guar gum –Based buoy bead flotation 瓜尔胶基浮筒珠浮可持续微藻收获方法研究

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-25 DOI: 10.1016/j.bej.2025.109883

Kemin Wei , Hao Wen , Meili Wu , Yue Wang , Jia He , Xichen Zheng , Xu Liu , Peng Wei , Shaoli Fan , Linjuan Zhang , Jian Gao

{"title":"A sustainable microalgae harvesting method via guar gum –Based buoy bead flotation","authors":"Kemin Wei , Hao Wen , Meili Wu , Yue Wang , Jia He , Xichen Zheng , Xu Liu , Peng Wei , Shaoli Fan , Linjuan Zhang , Jian Gao","doi":"10.1016/j.bej.2025.109883","DOIUrl":"10.1016/j.bej.2025.109883","url":null,"abstract":"<div><div>This study presents a novel plant-based flocculation–flotation strategy for harvesting microalgae, employing cationic cellulose nanofibers (CCNF) as flocculants and a crosslinked guar gum emulsion (GGE) as buoy beads. <em>Chlorella vulgaris</em> was chosen as a representative microalgae, and single-factor and response surface experiments were conducted to optimize harvesting conditions. The results showed that under optimal conditions, the harvesting efficiency of GGE reached 96.36 %, while the concentration factor was 2.01 %. Environmental and safety assessments demonstrated minimal ecological risk, with a carbon footprint of 0.664 kg CO₂ eq and a production cost of $2.44. Residual levels of harmful substances in the supernatant were negligible. Mechanism analysis demonstrated that CCNF neutralized the microalgae electrically, inducing flocculation, while GGE served as a binder, facilitating the flotation of the flocs. These findings highlight the potential of biodegradable, plant-based materials to enable high-efficiency, low-cost, and eco-friendly microalgae harvesting suitable for large-scale biofuel production.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109883"},"PeriodicalIF":3.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of C/N ratio and sulfate concentration on simultaneous partial nitrification and denitrifying phosphorus removal (SPNDPR) process C/N和硫酸盐浓度对同时部分硝化和反硝化除磷（SPNDPR）过程的影响

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-25 DOI: 10.1016/j.bej.2025.109885

Hui Jin , Tianqi Zhang , Yihan Cao , Jie Wang , Pengfei Shan , Chengchao Xin , Xiaoling Zhang , Aixia Chen , Wenjuan Yang

{"title":"Effects of C/N ratio and sulfate concentration on simultaneous partial nitrification and denitrifying phosphorus removal (SPNDPR) process","authors":"Hui Jin , Tianqi Zhang , Yihan Cao , Jie Wang , Pengfei Shan , Chengchao Xin , Xiaoling Zhang , Aixia Chen , Wenjuan Yang","doi":"10.1016/j.bej.2025.109885","DOIUrl":"10.1016/j.bej.2025.109885","url":null,"abstract":"<div><div>Simultaneous partial nitrification and denitrifying phosphorus removal (SPNDPR) is a promising strategy for treating low carbon-to-nitrogen (C/N) ratio wastewater. This study explored the establishment, performance, and microbial mechanisms of the SPNDPR process in a sequencing batch reactor (SBR) under varying C/N ratios (6.67–3.33) and sulfate concentrations (50–300 mg/L). Operated under low dissolved oxygen (DO) and intermittent aeration, the system achieved high removal efficiencies for ammonium nitrogen (NH₄⁺-N, 99.88 ± 0.55 %), total inorganic nitrogen (TIN, 79.90 ± 7.17 %), PO₄³ ⁻-P (91.67 ± 3.72 %), and chemical oxygen demand (COD, 92.60 ± 0.27 %). The ratio of ammonia-oxidizing bacteria (AOB, Nitrosomonas) to nitrite-oxidizing bacteria (NOB, Nitrospira) increased from 0.34 to 2.09, indicating successful partial nitrification. Polyphosphate-accumulating organisms (PAOs, <em>Candidatus Accumulibacter</em>) and glycogen-accumulating organisms (GAOs, <em>Candidatus Competibacter</em>) were effectively enriched, facilitating phosphorus removal and denitrifying activity. At a C/N ratio of 5, nutrient removal remained stable; however, further reduction to 3.33 led to decreased TIN (83.99 ± 6.34 %) and PO₄<sup>3-</sup>-P (88.08 ± 4.37 %) removal. Increasing influent sulfate concentrations adversely affected performance, with removal efficiencies of TIN and PO<sub>4</sub><sup>3-</sup>-P declining to 87.22 ± 3.57 % and 60.22 ± 9.47 %, respectively, at 300 mg/L. High sulfate levels also altered the microbial community, with sulfate-reducing bacteria (SRB), <em>Desulfomicrobium</em> (1.81 %) and <em>Desulfosporosinus</em> (5.01 %), becoming prevalent.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109885"},"PeriodicalIF":3.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144720913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep multi-kernel cell clustering for single-cell RNA sequencing data 单细胞RNA测序数据的深度多核细胞聚类

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-25 DOI: 10.1016/j.bej.2025.109877

Maoxuan Yao , Lina Ren

{"title":"Deep multi-kernel cell clustering for single-cell RNA sequencing data","authors":"Maoxuan Yao , Lina Ren","doi":"10.1016/j.bej.2025.109877","DOIUrl":"10.1016/j.bej.2025.109877","url":null,"abstract":"<div><div>Although existing deep learning methods for single-cell RNA sequencing (scRNA-seq) data can handle high-dimensional data and extract complex features, they also have the issue that the learned representations do not consider the clustering structure, leading to difficulties in linear separation. To address this, we propose a deep multi-kernel cell clustering network for scRNA-seq data, termed scDMKC, to cluster scRNA-seq data by simultaneously optimizing the multi-kernel representation of scRNA-seq data and the cell partitioning, enhancing the cell clustering performance by leveraging the strengths of multi-kernel learning and deep learning in capturing linearly separable data structures. To effectively learn the multi-kernel representation of scRNA-seq data, we propose a multi-kernel representation learner that adaptively selects an appropriate combination of multiple kernels to map the hidden representations of the data into the kernel space, thereby capturing the underlying linearly separable structure of the scRNA-seq data. In order to jointly optimize the multi-kernel representation of scRNA-seq data and the cell partitioning, a ZINB-based self-supervised strategy is developed. This strategy not only enhances the linear separability of the learned kernel representations but also improves clustering performance. Extensive experiments on various real scRNA-seq datasets were conducted. The experimental results indicate that our proposed scDMKC model significantly outperforms most of the existing cell clustering methods.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109877"},"PeriodicalIF":3.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144739213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive carboxylated PAN nanofiber membranes from expired egg white waste for antibacterial applications 用过期蛋清制备生物活性羧化聚丙烯腈纳米纤维膜用于抗菌

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-24 DOI: 10.1016/j.bej.2025.109882

Edouard Gnoumou , Thi Tam An Tran , Quang-Vinh Le , Nguyen The Duc Hanh , Nanthiya Hansupalak , Bing-Lan Liu , Chen-Yaw Chiu , Chi-Yun Wang , Kuei-Hsiang Chen , Yu-Kaung Chang

{"title":"Bioactive carboxylated PAN nanofiber membranes from expired egg white waste for antibacterial applications","authors":"Edouard Gnoumou , Thi Tam An Tran , Quang-Vinh Le , Nguyen The Duc Hanh , Nanthiya Hansupalak , Bing-Lan Liu , Chen-Yaw Chiu , Chi-Yun Wang , Kuei-Hsiang Chen , Yu-Kaung Chang","doi":"10.1016/j.bej.2025.109882","DOIUrl":"10.1016/j.bej.2025.109882","url":null,"abstract":"<div><div>The rapid expansion of the egg industry has led to the accumulation of expired egg white waste, much of which remains underutilized and contributes to environmental pollution. Given the natural antimicrobial proteins in chicken egg white (CEW), such as lysozyme (LYZ) and ovotransferrin, repurposing expired CEW offers a sustainable strategy to reduce waste while addressing concerns about antibiotic resistance. In this study, polyacrylonitrile (PAN) nanofiber membranes were functionalized with −COOH groups via alkaline hydrolysis to facilitate protein attachment. Expired CEW was immobilized onto the modified membranes through weak ionic and covalent interactions, forming P-COOH-EWP (egg white proteins) nanofiber membranes. The membranes were characterized for surface morphology, chemical composition, and antibacterial activity against <em>Escherichia coli</em> and <em>Staphylococcus aureus</em>. Additional evaluations included enzymatic activity, cytotoxicity, reusability, and storage stability. The results confirmed successful immobilization of EWP. The P-COOH-EWP membranes demonstrated vigorous antibacterial activity, with 86.57 % efficacy against <em>E. coli</em> and 75.05 % against <em>S. aureus</em>, and showed excellent biocompatibility without cytotoxic effects. They retained more than 72 % antibacterial activity after four weeks of storage at room temperature. However, activity decreased by 40 % after two uses, indicating suitability for single-use applications. This work presents a cost-effective and environmentally friendly approach to valorizing expired CEW waste into functional antibacterial nanomaterials for biomedical, food packaging, and environmental applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109882"},"PeriodicalIF":3.7,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144714481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green extraction and purification of phycocyanin from Spirulina platensis: Evaluation of its bioactivity and photoprotection 螺旋藻藻蓝蛋白的绿色提取纯化及其生物活性和光防护性评价

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-23 DOI: 10.1016/j.bej.2025.109881

Zahrotul Firdaus, Safrina Dyah Hardiningtyas , Iriani Setyaningsih

{"title":"Green extraction and purification of phycocyanin from Spirulina platensis: Evaluation of its bioactivity and photoprotection","authors":"Zahrotul Firdaus, Safrina Dyah Hardiningtyas , Iriani Setyaningsih","doi":"10.1016/j.bej.2025.109881","DOIUrl":"10.1016/j.bej.2025.109881","url":null,"abstract":"<div><div>Phycocyanin extracted from the microalga <em>Spirulina platensis</em> demonstrates diverse biological activities, with its effectiveness depending on its purity. This study focused on developing an eco-friendly extraction method by combining ultrasonication and freeze–thawing, establishing a purification approach using a combination of an aqueous two-phase system with a natural deep eutectic solvent (ATPS-NaDES) alongside precipitation techniques, and assessing phycocyanin’s antioxidant, UV-photoprotective, and antimicrobial properties relative to its purity. The findings revealed that the highest purity index (PI) of phycocyanin (2.49 ± 0.03), achieving cosmeceutical-grade quality with a recovery rate of 69.42 %, was obtained using the combined ATPS-NaDES and precipitation method (N + P). Phycocyanin demonstrated strong antioxidant activity, with IC₅₀ values of 38.15 ± 0.56 ppm for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 25.70 ± 0.25 ppm for ABTS, along with a Trolox equivalent value of 204.33 μg/mL at 75 ppm in the ferric reducing antioxidant power (FRAP) assay. Its UV-photoprotective effect was notable, showing a sun protection factor (SPF) of 40.23 ± 0.01 at 5 mg/mL. Additionally, phycocyanin exhibited effective antimicrobial activity against <em>Staphylococcus aureus</em>, <em>Escherichia coli</em>, and <em>Cutibacterium acnes</em>. This study highlights phycocyanin's multifunctional bioactivities, underscoring its potential as a natural antioxidant, photoprotective, and antimicrobial ingredient for cosmetic applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109881"},"PeriodicalIF":3.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144714479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0