Biochemical Engineering Journal最新文献_第5页

Volemitol production from Yarrowia lipolytica via transaldolase gene (TAL) disruption and erythrose-4-phosphate (E4P) flux regulation 通过反式脱醛酶基因 (TAL) 干扰和赤藓糖-4-磷酸 (E4P) 通量调节从脂肪分解酵母菌中产生油醇

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-23 DOI: 10.1016/j.bej.2024.109535

Liyun Ji, Qing Li, Ye Li, Shuo Xu, Hairong Cheng

{"title":"Volemitol production from Yarrowia lipolytica via transaldolase gene (TAL) disruption and erythrose-4-phosphate (E4P) flux regulation","authors":"Liyun Ji, Qing Li, Ye Li, Shuo Xu, Hairong Cheng","doi":"10.1016/j.bej.2024.109535","DOIUrl":"10.1016/j.bej.2024.109535","url":null,"abstract":"<div><div>Volemitol (D-<em>glycero</em>-D-<em>manno</em>-Heptitol, C<sub>7</sub>H<sub>16</sub>O<sub>7</sub> with CAS No.488–38–0), a seven-carbon (C7) sugar-derived alcohol, has the potential to be used as a natural sweetener. The natural scarcity of sugar alcohols restricts their practical uses. However, <em>Yarrowia lipolytica</em> has shown significant promise in industrial production due to its capability to efficiently produce sugar alcohols like erythritol, D-threitol, mannitol, and xylitol by generating key biosynthetic intermediates through glycolysis and the phosphopentose (PPP) pathway. In this study, the transaldolase gene (<em>TAL</em>) in the erythritol synthesis pathway was knocked out in the erythritol-producing <em>Y. lipolytica</em> strain CGMCC7326 to inhibit erythritol production. <em>TAL-</em>deleted strain <em>Y. lipolytica</em> CGMCC7326Δ<em>TAL</em> exhibited a notable decline in erythritol production; however, a novel substance, volemitol, was generated from glucose at a titer of 50 g/L. Volemitol with 99 % purity was obtained as a white microneedle powder crystal through the enzymatic activity of mannitol dehydrogenase (<em>MDH2</em>), which reduces sedoheptulose to volemitol. The proposed biosynthetic pathway in <em>Y. lipolytica</em> CGMCC7326Δ<em>TAL</em> is: sedoheptulose-7-phosphate was converted to sedoheptulose, then was reduced to volemitol. In conclusion, this study is the first to report efficient volemitol production from glucose via fermentation by engineered <em>Y. lipolytica</em>.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109535"},"PeriodicalIF":3.7,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced antimicrobial performance of single-use polyamide 56 nanofiber membranes modified with chitosan, reactive dyes, and poly(hexamethylene biguanide) (PHMB) for biological waste treatments 用壳聚糖、活性染料和聚六亚甲基双胍 (PHMB) 改性的一次性聚酰胺 56 纳米纤维膜的抗菌性能增强，可用于生物废物处理

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-22 DOI: 10.1016/j.bej.2024.109538

Jia-Hong Yang , Quang-Vinh Le , Bing-Lan Liu , Penjit Srinophakun , Chi-Yun Wang , Chen‑Yaw Chiu , I-Son Ng , Kuei-Hsiang Chen , Yu-Kaung Chang

{"title":"Enhanced antimicrobial performance of single-use polyamide 56 nanofiber membranes modified with chitosan, reactive dyes, and poly(hexamethylene biguanide) (PHMB) for biological waste treatments","authors":"Jia-Hong Yang , Quang-Vinh Le , Bing-Lan Liu , Penjit Srinophakun , Chi-Yun Wang , Chen‑Yaw Chiu , I-Son Ng , Kuei-Hsiang Chen , Yu-Kaung Chang","doi":"10.1016/j.bej.2024.109538","DOIUrl":"10.1016/j.bej.2024.109538","url":null,"abstract":"<div><div>Polyamide 56 (PA56), derived from renewable resources like wheat and corn via microbial fermentation, presents a sustainable alternative to traditional petrochemical-based polymers and has gained attention for its versatility in various fields. This study explores the antibacterial properties of bio-nylon nanofibers through fabrication and modification processes. PA56 nanofiber membrane was initially fabricated using electrospinning technology. These membranes were then modified with chitosan (CS), forming PA56-CS membranes, and further enhanced with Reactive dyes (Reactive Green 19, RG19 and Reactive Red 141, RR141) to produce PA56-CS-DYE membranes. Poly(hexamethylene biguanide) (PHMB), a positively charged polymer, was subsequently bonded to these membranes, resulting in PA56-CS-DYE-PHMB nanofiber membranes. Comprehensive physical characterizations using Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermogravimetric analysis (TGA) were conducted alongside qualitative and quantitative antibacterial assays against <em>E. coli</em>. The PA56-CS-RR141-PHMB nanofiber membrane exhibited exceptional antibacterial efficiency (<em>AE</em>), exceeding 97 %, indicating strong potential for biological waste treatment and related applications. However, the high antibacterial performance of these membranes is primarily suited for single-use systems, as their efficacy diminishes with repeated use, making them ideal for immediate and short-term applications in healthcare and environmental settings.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109538"},"PeriodicalIF":3.7,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent trends in metabolic engineering for microbial production of value-added natural products 微生物生产增值天然产品的代谢工程最新趋势

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-21 DOI: 10.1016/j.bej.2024.109537

Prasenjit Chakraborty , Randhir Kumar , Sanjay Karn , Payal Patel , Haren Gosai

{"title":"Recent trends in metabolic engineering for microbial production of value-added natural products","authors":"Prasenjit Chakraborty , Randhir Kumar , Sanjay Karn , Payal Patel , Haren Gosai","doi":"10.1016/j.bej.2024.109537","DOIUrl":"10.1016/j.bej.2024.109537","url":null,"abstract":"<div><div>Microbe-mediated chemical production is a sustainable approach for production of natural products that are important for our society. Traditional fermentation production of natural products <em>viz.</em> drugs, nutraceuticals, pigments, aromatic compounds, and biofuels has limitations such as low yield, high cost and time- consuming process. Significant success in the economical production of these natural products has been established by altering the metabolic pathways of microorganisms through metabolic engineering. Recent advancements in new tools and strategies have led to a significant reduction in the turnaround time in the classic approach i.e. design-build-test-learn (DBTL) cycle in metabolic engineering. Here, we summarize various tools and strategies developed in the area of metabolic engineering with a focus on value-added natural products Also, this review lists bioprospecting of microorganisms through metabolic engineering in last five years for production of natural products. At the end, outlook, challenges and opportunities for the successful establishment of robust engineered microbial cell factories have been reported.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109537"},"PeriodicalIF":3.7,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142586054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assembly of a tumor microtissue by stacking normal and cancer spheroids on Kenzan using a bio-3D printer to monitor dynamic cancer cell invasion in the microtissue 使用生物三维打印机将正常球体和癌症球体堆叠在肯赞上，组装成肿瘤微组织，以监测微组织中癌细胞的动态侵袭情况

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-19 DOI: 10.1016/j.bej.2024.109536

Kazuaki Ninomiya , Tatsuhiko Taniuchi

{"title":"Assembly of a tumor microtissue by stacking normal and cancer spheroids on Kenzan using a bio-3D printer to monitor dynamic cancer cell invasion in the microtissue","authors":"Kazuaki Ninomiya , Tatsuhiko Taniuchi","doi":"10.1016/j.bej.2024.109536","DOIUrl":"10.1016/j.bej.2024.109536","url":null,"abstract":"<div><div>In the present study, a tumor microtissue was assembled by precisely stacking normal and cancer cell spheroids on Kenzan (microneedle array) using a spheroid stacking type bio-3D printer. This is the first study to non-invasively observe the dynamic behavior of GFP-tagged cancer cell invasion in the microtissue assembled by a spheroid stacking type bio-3D printer. First, the cancer cell spheroid was prepared using 10 % cancer cells (MCF-7 expressing GFP) and 90 % normal cells (70 % HNDF and 20 % HUVEC). The normal cell spheroid was prepared using 100 % normal cells (80 % HNDF and 20 % HUVEC). The tumor microtissue was then assembled on Kenzan by placing 1 cancer cell spheroid in the center position of the microtissue and 8 normal cell spheroids around it. 9 spheroids stacked on Kenzan were fused into 1 tumor microtissue after 24 hours of culture. The green fluorescence derived from cancer cells spread from the central position to the entire area of the tumor microtissue. The spread dynamics of cancer cell-derived GFP fluorescence can be used as a simple measure to evaluate cancer cell migration/invasion and response to anticancer drugs.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"212 ","pages":"Article 109536"},"PeriodicalIF":3.7,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142532102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adsorption characteristics of virus-mimetic fluorescent nanoparticles on polymer fiber material surfaces 仿病毒荧光纳米粒子在聚合物纤维材料表面的吸附特性

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-19 DOI: 10.1016/j.bej.2024.109534

Rina Uchida , Ayuri Mitsuno , Tomohiro Komatsu , Chisato Sakamoto , Satoshi Amaya , Satoshi Migita , Eiichiro Takamura , Hiroaki Sakamoto

{"title":"Adsorption characteristics of virus-mimetic fluorescent nanoparticles on polymer fiber material surfaces","authors":"Rina Uchida , Ayuri Mitsuno , Tomohiro Komatsu , Chisato Sakamoto , Satoshi Amaya , Satoshi Migita , Eiichiro Takamura , Hiroaki Sakamoto","doi":"10.1016/j.bej.2024.109534","DOIUrl":"10.1016/j.bej.2024.109534","url":null,"abstract":"<div><div>Contact infection, whereby the virus is transmitted through objects to which it adheres, affects the spread of infection. Although inactivation with alcohol is commonly used, the virus may adhere to the surface again after removal. It is necessary to take measures against direct and contact infection to control the spread of viral infection. However, the type of material the viruses adsorb onto is not fully understood. The objective in the present study is to elucidate virus adsorption behavior to suppress the indirect spread of infection. We examined for materials that are difficult for viruses to adhere to and investigated their adsorption mechanisms to control the spread of infection through contact infection. In this study, spike protein-modified fluorescent nanoparticles (SFNs) were designed and fabricated by modifying the surface of fluorescent particles with the spike S1 protein, which is expected to first contact and adsorb onto the material surface. Purpose of the present study is to analyze the influence of material characteristics on virus adhesion using SFNs. SFNs were adsorbed onto Nylon, polyester (PET), polypropylene (PP), polytetrafluoroethylene (PTFE), acrylic and rayon, and their fluorescence intensities and adsorption characteristics were compared by scanning electron microscopy (SEM) analysis and fluorescence analysis. Principal component analysis showed that virus adsorption was more sensitive to coarseness for PTFE than for other fibers.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109534"},"PeriodicalIF":3.7,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Process modeling and 3-stage photobioreactor design for algae cultivation and CO2 capture: A case study using palm oil mill effluent 用于藻类培养和二氧化碳捕获的工艺建模和三阶段光生物反应器设计：利用棕榈油厂废水的案例研究

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-18 DOI: 10.1016/j.bej.2024.109532

Emmanuel Yahaya , Wan Sieng Yeo , Jobrun Nandong

{"title":"Process modeling and 3-stage photobioreactor design for algae cultivation and CO2 capture: A case study using palm oil mill effluent","authors":"Emmanuel Yahaya , Wan Sieng Yeo , Jobrun Nandong","doi":"10.1016/j.bej.2024.109532","DOIUrl":"10.1016/j.bej.2024.109532","url":null,"abstract":"<div><div>Microalgae have advantages, including rapid growth rates, a high lipid production capacity, effective removal of nitrates and phosphates from wastewater, and efficient carbon dioxide (CO<sub>2</sub>) absorption. The optimal operating conditions and strategies of microalgae cultivation can vary significantly from one goal to another. An economic approach to exploring various operating strategies is doable via microalgal process modeling and simulation. Therefore, this study aims to develop a simulation model aimed at enhancing algae growth within a photobioreactor (PBR) system designed to reduce CO<sub>2</sub> emissions in palm oil mills. This simulation model is constructed to explore the algae growth CO<sub>2</sub> capture efficiency and the influence of oxygen (O<sub>2</sub>) in the water in the PBR. This study achieved a CO<sub>2</sub> capture efficiency of up to 60 % which represents the highest capture, and a dissolved O<sub>2</sub> of 20 % was achieved due to the effect of the mass transfer coefficient. Algal growth exhibited a high rate, approximately 1057 g/m<sup>3</sup>, which could serve as a potential pathway for biodiesel or biobutanol production. Additionally, this study underscores the significant role of the mass transfer coefficient in effectively reducing liquid O<sub>2</sub> levels to maximize CO<sub>2</sub> capture and achieve a high algae yield. Furthermore, the simulation results reveal that a high concentration of O<sub>2</sub> in the water promotes photorespiration, which hampers algal growth and reduces CO<sub>2</sub> capture efficiency.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"212 ","pages":"Article 109532"},"PeriodicalIF":3.7,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142532101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced catalytic activity and reusability of sucrose phosphorylase@magnetic nanoparticles by surface-coating amorphous ZIF-67 通过表面包覆无定形 ZIF-67 增强蔗糖磷酸化酶@磁性纳米粒子的催化活性和重复使用性

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-16 DOI: 10.1016/j.bej.2024.109533

Zhendong Liu , Haichang Xu , Bin Wei , Hao Liang

{"title":"Enhanced catalytic activity and reusability of sucrose phosphorylase@magnetic nanoparticles by surface-coating amorphous ZIF-67","authors":"Zhendong Liu , Haichang Xu , Bin Wei , Hao Liang","doi":"10.1016/j.bej.2024.109533","DOIUrl":"10.1016/j.bej.2024.109533","url":null,"abstract":"<div><div>Sucrose phosphorylase (SPase) is a highly efficient glycosyltransferase which has a wide range of substrate specificity and excellent application prospects in the cosmetics, food, and medicine fields. The application of free SPase is limited due to its high cost, poor stability and poor reusability. Immobilization of enzymes can solve these problems. In this study, SPase was firstly immobilized by magnetic nanoparticles (MNPs) and surface-coating amorphous ZIF-67 for enhancing catalytical activity and reusability. ZIF-67@SPase@MNPs with MNPs core and ZIF-67 shell was characterized by TEM, SEM, XRD, FT-IR and VSM. Compared with free SPase, the catalytic activity of ZIF-67@SPase@MNPs increased by 30 %. Coated with amorphous ZIF-67, the immobilized enzyme retained 70 % relative activity after 12 cycles and 80 % relative activity after 15 days of storage. In addition, ZIF-67@SPase@MNPs had strong magnetic properties and the saturation magnetization was 52.07 emu/g. Surface-coating amorphous ZIF-67 on SPase@MNPs is a promising method for immobilizing enzymes, which can improve catalytic activity and reusability, therefore showing great application potential in biocatalysts and product separation. It has a good application prospect in the production of high viscosity glycosides.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"212 ","pages":"Article 109533"},"PeriodicalIF":3.7,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142532100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Removal of selenate from wastewater using a bioelectrochemical reactor: The importance of measuring selenide and the role of competing anions 利用生物电化学反应器去除废水中的硒酸盐：测量硒化物的重要性和竞争阴离子的作用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-15 DOI: 10.1016/j.bej.2024.109531

Benhur K. Asefaw , Huan Chen , Youneng Tang

{"title":"Removal of selenate from wastewater using a bioelectrochemical reactor: The importance of measuring selenide and the role of competing anions","authors":"Benhur K. Asefaw , Huan Chen , Youneng Tang","doi":"10.1016/j.bej.2024.109531","DOIUrl":"10.1016/j.bej.2024.109531","url":null,"abstract":"<div><div>Removal of selenate (SeO<sub>4</sub><sup>2-</sup>) from selenate-contaminated wastewater is challenging due to the commonly co-existing and competing anions of sulfate (SO<sub>4</sub><sup>2-</sup>) and nitrate (NO<sub>3</sub><sup>-</sup>). This study investigates SeO<sub>4</sub><sup>2−</sup> reduction to elemental selenium (Se<sup>0</sup>) in a cathode-based bioelectrochemical (BEC) reactor and a conventional biofilm reactor (<em>i.e.</em>, an upflow anaerobic reactor). The simulated wastewater contained SeO<sub>4</sub><sup>2−</sup> at a typical concentration of 5 mg Se/L, SO<sub>4</sub><sup>2−</sup> at a typical concentration of 1000 mg S/L, and NO<sub>3</sub><sup>−</sup> at concentrations that varied from 0 to 10 mg N/L. The impact of sulfate on the BEC reactor was much lower than that on the conventional reactor: The selenium removal, defined as (selenate in influent – dissolved selenium in effluent)/selenate in influent, was 99 % in the BEC reactor versus 69 % in the conventional biofilm reactor. The lower selenium removal in the conventional reactor was mainly due to the >10 times higher reduction of sulfate, which directly caused competition between sulfate and selenate for the common resources such as electrons. The more reduction of sulfate in the conventional reactor further led to 45 times higher production of selenide. Selenide is usually assumed to be minimal and therefore not measured in the literature. This simplification may significantly overestimate selenium removal when the influent sulfate concentration is very high. NO<sub>3</sub><sup>-</sup> in the influent of the BEC reactor promoted selenium removal when it was less than 5.0 mg N/L but inhibited selenate removal when it was more than 7.5 mg N/L. This was supported by the microbial community analysis and intermediate (nitrite) analysis.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"212 ","pages":"Article 109531"},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corn stover waste preparation cerium-modified biochar for phosphate removal from pig farm wastewater: Adsorption performance and mechanism 玉米秸秆废料制备的铈改性生物炭用于去除养猪场废水中的磷酸盐：吸附性能和机理

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-13 DOI: 10.1016/j.bej.2024.109530

Liu Lou , Weikun Li , Hao Yao , Huiying Luo , Gang Liu , Jun Fang

{"title":"Corn stover waste preparation cerium-modified biochar for phosphate removal from pig farm wastewater: Adsorption performance and mechanism","authors":"Liu Lou , Weikun Li , Hao Yao , Huiying Luo , Gang Liu , Jun Fang","doi":"10.1016/j.bej.2024.109530","DOIUrl":"10.1016/j.bej.2024.109530","url":null,"abstract":"<div><div>The high phosphorus content in livestock and poultry wastewater is a significant factor contributing to water eutrophication. It is imperative to seek an economically efficient method for phosphate recovery. This study employed cerium-modified biochar to recover phosphate from pig farm wastewater. An investigation was conducted to examine the adsorption performance and removal mechanism of phosphate. Among the different samples, 0.1CeB-500℃ was selected for subsequent experiments. It exhibited a phosphate adsorption capacity of 9.58 mg/g and a removal efficiency of 95.75 %. The results showed that the phosphate adsorption process followed not only the pseudo-second-order kinetic model, but also the Langmuir isotherm model. It suggested that the adsorption of phosphate onto the biochar occurred in a monolayer chemical manner, with a maximum adsorption capacity of 10.86 mg/g. Phosphate adsorption was minimally affected within the pH range of 2–9, with Cl<sup>-</sup> having negligible impact, NO<sub>3</sub><sup>-</sup> slightly inhibiting, and HCO<sub>3</sub><sup>-</sup> and CO<sub>3</sub><sup>2-</sup> significantly hindering phosphate adsorption. A series of characterization results indicated that phosphate removal occurred through surface precipitation forming CePO<sub>4</sub>, ligand exchange between carbonate and phosphate, inner-sphere complexation, and electrostatic attraction. The phosphate removal efficiency from pig farm wastewater was 43.25 %, demonstrating promising potential for practical application.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"212 ","pages":"Article 109530"},"PeriodicalIF":3.7,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Power production characteristics of binary particles pulsed anaerobic fluidized bed microbial fuel cell 二元颗粒脉冲厌氧流化床微生物燃料电池的发电特性

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-10-11 DOI: 10.1016/j.bej.2024.109524

Yangfan Song, Meng Wang, Yiming Han, Yanmin Li, Hongwei Chen, Xiang Wei, Zhuo Liu

{"title":"Power production characteristics of binary particles pulsed anaerobic fluidized bed microbial fuel cell","authors":"Yangfan Song, Meng Wang, Yiming Han, Yanmin Li, Hongwei Chen, Xiang Wei, Zhuo Liu","doi":"10.1016/j.bej.2024.109524","DOIUrl":"10.1016/j.bej.2024.109524","url":null,"abstract":"<div><div>A novel binary particulate pulsating anaerobic fluidized bed microbial fuel cell (BPFB-MFC) was designed and constructed in order to improve the efficiency of low-grade energy conversion in sewage. The effects of pulsed liquid flow rate and bed filling rate on the electricity production performance and effluent treatment characteristics of the BPFB-MFC were investigated experimentally. The results showed that when the pulsed liquid flow was <em>u</em>=1.95sin(π/3)<em>t</em> cm·s<sup>−1</sup> and when the bed materials in the anode chamber consisted of 10 % bed height activated carbon particles and 10 % bed height ceramic particles, the highest voltage produced was 519.7 mV, the highest power density was 587.5 mW·m<sup>−2</sup>, and the lowest internal resistance was 171.2Ω, which was the optimal experimental working condition. It was found that the electricity production performance and effluent treatment efficiency of the mixed particles system were better than those of a system with single particles. This work held promise of promoting the industrialization of MFC.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"212 ","pages":"Article 109524"},"PeriodicalIF":3.7,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142532099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0