Biochemical Engineering Journal最新文献_第5页

Growth and biochemical composition of Limnospira fusiformis cultivated under simulated outdoor light intensity in photobioreactors 在光生物反应器中模拟室外光照强度下培养的鱼腥褐藻的生长和生化组成

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-09-03 DOI: 10.1016/j.bej.2024.109482

{"title":"Growth and biochemical composition of Limnospira fusiformis cultivated under simulated outdoor light intensity in photobioreactors","authors":"","doi":"10.1016/j.bej.2024.109482","DOIUrl":"10.1016/j.bej.2024.109482","url":null,"abstract":"<div>Outdoor cultivation using natural sunlight efficiently produces valuable microalgal products, such as proteins, lipids, carbohydrates, and antioxidants but photoinhibition from intense sunlight must be minimized. This study explores the effect of varying simulated outdoor light intensity on Limnospira fusiformis growth and biochemical composition. Four light scenarios were tested to simulate varying outdoor light conditions: full sunlight (2000 µmol m⁻²s⁻¹), greenhouse (1700 µmol m⁻²s⁻¹), mid-day shade in a greenhouse (1400 µmol m⁻²s⁻¹), and whole-time shade in a greenhouse (1400 µmol m⁻²s⁻¹). Whole-time shade yielded the highest last-day dry weight (2.10 g L⁻¹), protein content (63.10 % ash-free dry weight), phycocyanin productivity (0.11 g L⁻¹d⁻¹), and lowest ash accumulation (11.00 %). High light intensity led to substantial carbohydrate accumulation, while protein synthesis and cell growth declined. This study is the first to report the correlation between high light-induced morphological changes with both protein and phycocyanin levels. Shading techniques enhanced biomass production and composition in Limnospira fusiformis. The observed improvements in protein content and phycocyanin productivity under specific light conditions demonstrate the potential for optimizing outdoor cultivation of indigenous microalgal strains, contributing to more efficient and sustainable production methods for industrial applications.</div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142149384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-lasting degradation of long-chain alkanes through activating Bacillus-like microbes after Fenton pre-oxidation in soil 在土壤中进行 Fenton 预氧化后，通过激活类芽孢杆菌微生物长效降解长链烷烃

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-09-02 DOI: 10.1016/j.bej.2024.109481

{"title":"Long-lasting degradation of long-chain alkanes through activating Bacillus-like microbes after Fenton pre-oxidation in soil","authors":"","doi":"10.1016/j.bej.2024.109481","DOIUrl":"10.1016/j.bej.2024.109481","url":null,"abstract":"<div>To explore the effects and mechanisms of long-lasting degradation of long-chain alkanes (C25-C30) in petroleum-contaminated soil, a solid iron catalyst prepared by adding different proportions of (5 % and 15 % (w/w)) chitosan (CS) was used for Fenton pre-oxidation experiment. Bioremediation experiments were performed for 100 days after pre-oxidation. The results indicated that the degradation for long-chain alkanes and Total Petroleum Hydrocarbons (TPH) were 76.95 % and 76.89 %, respectively. Furthermore, long-lasting degradation of long-chain alkanes was achieved by activating Bacillus-like microbes. In each biodegradation cycle, the long-chain alkanes degradation in the active control group increased by 77.39 mg/kg, 76.74 mg/kg, 36.88 mg/kg, and 76.51 mg/kg compared to the previous cycle. Besides, the half-life of long-chain alkanes was 131 days shorter in the active control group than in the inactive control group. Higher microbial enzyme activity for degrading long-chain alkanes was observed after Fenton pre-oxidation because the expression of alkane metabolism genes was activated by the high consumption of dissolved organic carbon. Finally, the dominant bacterial genera in the active control group shifted predominantly to Paenibacillus (13.26 %), Acinetobacter (8.02 %), and Microbacterium (17.64 %). Therefore, this study possesses significant engineering application value.</div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142149385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering microfluidic devices to mimic signaling cascades in continuous-flow cell culture as multiorgan microphysiological systems 设计微流控装置，模拟连续流细胞培养中的信号级联，作为多器官微观生理系统

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-08-30 DOI: 10.1016/j.bej.2024.109475

{"title":"Engineering microfluidic devices to mimic signaling cascades in continuous-flow cell culture as multiorgan microphysiological systems","authors":"","doi":"10.1016/j.bej.2024.109475","DOIUrl":"10.1016/j.bej.2024.109475","url":null,"abstract":"<div>The inability of traditional pre-clinical cell culture and animal models to accurately replicate human diseases and drug toxicities leads to a significant halt in the advancement of effective treatment strategies, in addition to financial losses. This, combined with the rise in ethical concerns about animal welfare, highlights the need for alternative and more realistic representations of human physiology. Microfluidics-based multiorgan microphysiological systems present a promising avenue for studying human body homeostasis, and have the potential to revolutionize translational research by creating new opportunities to comprehend systemic diseases and develop personalized medicine. In this review, we describe important design and operational considerations for engineering microfluidic devices mimicking tissue/organ “cross-talk” for in vitro drug disposition and safety assessments, as well as in disease modeling. We conducted a meticulous analysis of relevant articles and calculated crucial parameters, like the Reynolds number and shear stress, to compare the operational characteristics of different microfluidic devices. Additionally, we provide the reader with perspectives on the current limitations, insights to address the pending issues, and describe future opportunities of these technologies in the clinical setting.</div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142149383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of genetic code expansion to regulate the synthesis of poly(lactate-co-3-hydroxybutyrate) in Escherichia coli 应用遗传密码扩展来调节大肠杆菌中聚（乳酸-3-羟基丁酸）的合成

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-08-28 DOI: 10.1016/j.bej.2024.109478

{"title":"Application of genetic code expansion to regulate the synthesis of poly(lactate-co-3-hydroxybutyrate) in Escherichia coli","authors":"","doi":"10.1016/j.bej.2024.109478","DOIUrl":"10.1016/j.bej.2024.109478","url":null,"abstract":"<div>Genetic codon expansion has the potential to introduce a variety of unnatural amino acids to specific sites within target proteins. In this study, genetic codon expansion was employed to regulate the enzyme expression in metabolic pathways. Firstly, a purple protein from Actinia tenebrosa was selected as the candidate to be engineered. Bringing in UAG stop codon caused premature termination of translation, while expressing orthogonal aminoacyl-tRNA synthetase and tRNA from Methanococcus jannaschii restored translation at UAG site. However, leakage expression was observed without addition of unnatural amino acids, still it can be decreased by increasing numbers of UAG mutations. Subsequently, poly(lactate-co-3-hydroxyburyrate) [P(LA-3HB)] biosynthesis pathway was constructed in Escherichia coli, and propionyl-CoA transferase was mutated to harboring one or two more stop codons. With genetic codon expansion tools, the function of propionyl-CoA transferase was restored, promoting cells to synthesize P(LA-3HB) copolymer. Moreover, the lactate monomer content was regulated ranging from 0 to 33.42 mol% by altering the addition time of inducers. Finally, the strain accumulated 27.09 g/L P(25.1 mol% LA-3HB) in 5-L bioreactor cultivation. This is the first report on metabolic engineering of polyhydroxyalkanoate biosynthesis through genetic codon expansion and would provide helpful strategies to achieve dynamic regulation of multiple metabolic pathways.</div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142095607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stepwise cell culture process intensification for high-productivity and cost-effective commercial manufacturing of a Mabcalin™ bispecifics 逐步强化细胞培养工艺，实现高生产率和高成本效益的马布卡林™双特异性药物商业化生产

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-08-28 DOI: 10.1016/j.bej.2024.109476

{"title":"Stepwise cell culture process intensification for high-productivity and cost-effective commercial manufacturing of a Mabcalin™ bispecifics","authors":"","doi":"10.1016/j.bej.2024.109476","DOIUrl":"10.1016/j.bej.2024.109476","url":null,"abstract":"<div>Process intensification and media optimization, as a crucial step for improving productivity and manufacturing cost of goods (COG), set the stage for commercialization readiness and redefine the landscape for patient access. This study described a stepwise approach to explore different intensified fed-batch processes along with optimized cell culture media for the production of a Mabcalin™ bispecifics. Initially, by leveraging perfusion expansion, intensified fed-batch (IFB) with an inoculation density of 10.3 × 106 cells/mL was developed to produce 6.1 g/L of products, compared to 3.9 g/L from the original traditional fed-batch (TFB). Following the IFB conversion, a high-performing production medium, MagniCHO™, was chosen to substitute the original one, which further boosted the titer to 9.1 g/L. The result underscored the significance of developing an optimized cell culture media for intensified cultivation. Furthermore, the approach of ultra-intensified intermittent-perfusion fed-batch was utilized, raising the seeding density to 73.6 × 106 cells/mL. A final harvest titer of 24.5 g/L was recorded. Additionally, manufacturing COG was calculated to evaluate how process intensification could lead to improved manufacturing cost-effectiveness, with up to 71 % COG reduction attainable with the UI-IPFB process. This study demonstrated that even for difficult-to-express modalities, applying a strategic development approach including process intensification and media optimization could effectively improve manufacturing efficiency and COG competitiveness.</div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR: The frontier technology of next-generation RNA detection CRISPR：下一代 RNA 检测的前沿技术

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-08-28 DOI: 10.1016/j.bej.2024.109480

引用次数: 0

Engineering of Saccharomyces cerevisiae towards synthesis of linalool using linalool synthase from Magnolia champaca 利用木兰花中的芳樟醇合成酶对酿酒酵母进行工程改造以合成芳樟醇

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-08-28 DOI: 10.1016/j.bej.2024.109477

{"title":"Engineering of Saccharomyces cerevisiae towards synthesis of linalool using linalool synthase from Magnolia champaca","authors":"","doi":"10.1016/j.bej.2024.109477","DOIUrl":"10.1016/j.bej.2024.109477","url":null,"abstract":"<div>Linalool is one of the commercially important fragrance molecule usually extracted from Lavandula angustifolia (lavender) and Ocimum basilicum (basil) plants. In the present study, efforts were made to produce this molecule in microbial system to meet demand-supply imbalance. Linalool synthase (LIS) gene from Magnolia champaca (Mc) and Coriandrum sativum (Cs) were successfully cloned and expressed in Saccharomyces cerevisiae CEN PK2–1 C. It was observed that expression of full-length LIS (fLIS) resulted in lesser linalool when compared to truncated LIS (tLIS) devoid of plastid signal for both Mc and Cs. In terms of linalool yield, MctLIS resulted in 1.27-fold higher linalool when compared to CstLIS. Later, when two more genes viz., TPI1 and ALD6 which presumably increase sterol pathway flux were overexpressed, actually resulted in lower linalool and increased acetate production. However, multicopy expression of MctLIS and tHMG1 in this strain has reversed the above phenomenon due to presumptive push-pull strategy. Finally, this engineered strain was cultivated in the 2 L bioreactor in fed-batch mode to obtain 10.85 µg/mL of linalool. Docking studies of homology model of MctLIS with geranyl pyrophosophate (GPP) revealed V387, Y361, T434, R427 and R249 as key interactions sites. The study reports the linalool production using LIS gene from Magnolia champaca for the first time and could be a potential chassis for further studies.</div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142122334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-omics data and model integration reveal the main mechanisms associated with respiro-fermentative metabolism and ethanol stress responses in Kluyveromyces marxianus 多组学数据和模型整合揭示了马氏克鲁维氏菌呼吸发酵代谢和乙醇胁迫响应的主要机制

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-08-24 DOI: 10.1016/j.bej.2024.109471

{"title":"Multi-omics data and model integration reveal the main mechanisms associated with respiro-fermentative metabolism and ethanol stress responses in Kluyveromyces marxianus","authors":"","doi":"10.1016/j.bej.2024.109471","DOIUrl":"10.1016/j.bej.2024.109471","url":null,"abstract":"<div>Kluyveromyces marxianus is a yeast capable of fermenting sugars into ethanol and growing at high temperatures (>37ºC). However, it is less tolerant to ethanol than Saccharomyces cerevisiae, which limits its application in second-generation ethanol production. Since the mechanisms of ethanol stress response are still poorly described, especially compared to S. cerevisiae, we used an integrative multi-omics approach, combining transcriptomics, co-expression networks, gene regulation, and genome-scale metabolic modelling to gain insights about these mechanisms. Through metabolic modelling, we predicted the occurrence of a respiro-fermentative metabolism and its onset as the dilution rate increased. From gene co-expression networks, we detected that the protein quality control system is a main mechanism involved in the ethanol stress response. Further, we identified key regulators in the ethanol stress response, such as HAP3, MET4, and SNF2, and assessed how disturbances in their gene expression affect cellular metabolism. We also found that amino acid metabolism, membrane lipid metabolism, and ergosterol exhibit increased metabolic flux under the explored conditions, along with usage of enzymes related to these pathways. These findings provide useful cues to develop and implement genetic and metabolic engineering strategies to enhance ethanol tolerance and point for future research in stress responses of K. marxianus.</div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect and mechanism of steel slag composition on CO2 fixation rate under microbial and non-microbial 钢渣成分对微生物和非微生物条件下二氧化碳固定率的影响及其机理

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-08-23 DOI: 10.1016/j.bej.2024.109467

{"title":"Effect and mechanism of steel slag composition on CO2 fixation rate under microbial and non-microbial","authors":"","doi":"10.1016/j.bej.2024.109467","DOIUrl":"10.1016/j.bej.2024.109467","url":null,"abstract":"<div>In this study, we used microorganisms and steel slag to reduce CO2 emissions. The main objective is to investigate the influence and mechanisms of CO2 fixation rate based on the composition of steel slag. In the absence of microorganisms, steel slag powders with higher C2S content exhibit higher CO2 fixation rate. The absolute content of C2S decreases by 2.16–5.86 % and 3.43–14.21 % at 2 h and 48 h of carbon sequestration reaction, respectively. Under the action of microorganisms, the CO2 fixation rate of different steel slags increases by more than two-fold, with increases in amount of CO2 fixation at 2 h and 48 h of reaction being 142–169 % and 166–191 %, respectively. Microorganisms can enhance the reaction degree of C2S, C3S, and C2F phases in different steel slags. The increase in amount of CO2 fixation is particularly significant for steel slag powders with high C2S and C2F content. Enzymes secreted by microorganisms in the early stage of carbon sequestration can also increase the concentration of HCO3- and CO32- in the liquid phase, but this is influenced by the pH value and Ca2+ concentration of different steel slag leachates. Steel slag powders with lower leachate pH values and containing small amounts of Ca2+ will be more conducive to microorganisms enhancing the early-stage CO2 fixation rate.</div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142048696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term and high-efficiency capture of Escherichia coli using cellulose acetate nanofiber membrane functionalized with reactive 19 dye and polyhexamethylene biguanide 使用活性 19 染料和聚六亚甲基双胍功能化的醋酸纤维素纳米纤维膜长期高效捕获大肠杆菌

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-08-23 DOI: 10.1016/j.bej.2024.109474

{"title":"Long-term and high-efficiency capture of Escherichia coli using cellulose acetate nanofiber membrane functionalized with reactive 19 dye and polyhexamethylene biguanide","authors":"","doi":"10.1016/j.bej.2024.109474","DOIUrl":"10.1016/j.bej.2024.109474","url":null,"abstract":"<div>Cellulose acetate (CA) nanofibers have been popularly applied in various biomedical and textile products. In this work, a textile azo-dye Reactive Green 19 (RG19) was selected to be chemically coupled to the CA nanofiber membrane to form dyed CA nanofiber membrane (namely CA-RG19) and then poly(hexamethylene biguanide) (PHMB) as an antibacterial reagent was physically attached to the dyed CA nanofiber membrane, forming CA-RG19-PHMB nanofiber membrane. The nanofiber membranes were evaluated for their physical and mechanical properties, including functional group analysis, morphological characterization, and thermal stability assessment. To investigate the antibacterial properties of the nanofiber membrane, various concentrations of RG19 dye and PHMB were tested to evaluate the antibacterial efficiency (AE) against Escherichia coli of the membranes. It was found that the CA-RG19-PHMB nanofiber membrane exhibited an AE value of approximately 100 %, with the immobilization concentrations of RG19 dye and PHMB being 373.46 mg/g and 0.333 mg/g, respectively. The CA-RG19-PHMB nanofiber membrane showed 100 % antibacterial efficacy after 10 min against E. coli cells. Furthermore, the storage stability of the CA-RG19-PHMB nanofiber membrane remained at approximately 100 % of its initial antibacterial efficacy after 60 days, and it exhibited excellent antibacterial efficacy after five cycles.</div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0