Biochemical Engineering Journal最新文献_第6页

Enhanced lipid production in Chlorella vulgaris via indole-3-acetic acid salt stress in a two-stage culture for biofuels 二阶段生物燃料培养中吲哚-3-乙酸盐胁迫下普通小球藻脂质生产的增强

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-16 DOI: 10.1016/j.bej.2025.109795

Qun Wei , Dan Zhao , Ming Wang , Conghan Wang , Fu Pang , Xiangmeng Ma

{"title":"Enhanced lipid production in Chlorella vulgaris via indole-3-acetic acid salt stress in a two-stage culture for biofuels","authors":"Qun Wei , Dan Zhao , Ming Wang , Conghan Wang , Fu Pang , Xiangmeng Ma","doi":"10.1016/j.bej.2025.109795","DOIUrl":"10.1016/j.bej.2025.109795","url":null,"abstract":"<div><div>Utilizing saline wastewater for the cultivation of microalgae represents a viable approach to decrease the production costs associated with microalgae-derived biodiesel. In this study, a new method of combining plant hormones with two-stage culture was proposed to drive efficient lipid production of microalgae under salt stress. The findings indicated that the introduction of 40 mg·L<sup>−1</sup> of indole-3-acetic acid (IAA) during the initial phase resulted in optimal cell density and lipid productivity of <em>Chlorella vulgaris</em> under conditions of 10 g·L<sup>−1</sup> NaCl stress, achieving values of 65.15 × 10<sup>6</sup> cells·mL<sup>−1</sup> and 47.62 mg·L<sup>−1</sup>·d<sup>−1</sup>, respectively. In the second stage, the application of salt stress was repeated, leading to a lipid productivity of 56.17 mg·L<sup>−1</sup>·d<sup>−1</sup>, which represents an increase of 150.8 % compared to the untreated control group. Additionally, the proportion of saturated fatty acids rose to 29.26 %. Therefore, it has better combustion characteristics and stability. At the same time, the self-flocculation efficiency of microalgae also increased by 93.75 %. In addition, the regulatory mechanism of IAA on the growth and lipid accumulation of <em>Chlorella</em> under salt stress was discussed by quantitative analysis of the expression of genes related to signaling pathways and lipid biosynthesis in algal cells. This study provides ideas for microalgae to produce biodiesel and achieve sustainable development.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109795"},"PeriodicalIF":3.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-purpose immobilization of lipase onto modified magnetic nanoparticles: Characterization and enhancement of enzymatic interesterification 改良磁性纳米颗粒固定化脂肪酶的多用途研究：酶催化活性的表征和增强

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-16 DOI: 10.1016/j.bej.2025.109794

Hui Fang , Yuanyuan Deng , Zhihao Zhao, Jiarui Zeng, Mingwei Zhang, Pengfei Zhou

{"title":"Multi-purpose immobilization of lipase onto modified magnetic nanoparticles: Characterization and enhancement of enzymatic interesterification","authors":"Hui Fang , Yuanyuan Deng , Zhihao Zhao, Jiarui Zeng, Mingwei Zhang, Pengfei Zhou","doi":"10.1016/j.bej.2025.109794","DOIUrl":"10.1016/j.bej.2025.109794","url":null,"abstract":"<div><div>Improving catalytic performance represents a highly desirable but challenging objective for the immobilization of biocatalysts. A novel biocatalyst of ANL@Fe<sub>3</sub>O<sub>4</sub>-SiO<sub>2</sub>-APTES was fabricated by immobilizing <em>Aspergillus niger</em> lipase (ANL) onto amino-alkyl group-modified magnetic nanoparticles. This biocatalyst exhibited an enzyme loading of 127.68 mg/g and the specific activity of 1132.26 U/g. The superior activity and recyclability of ANL@Fe<sub>3</sub>O<sub>4</sub>-SiO<sub>2</sub>-APTES was validated. The outstanding performance for the enzymatic interesterification (EIE) of palm stearin (PS) and rice bran oils (RBO) was observed. The significantly decreased the solid fat content (SFC) and enhanced crystallization rate of oils was achieved, as well as the β' type of oils after EIE was obtained. Moreover, molecular dynamics simulations were conducted to elucidate the underlying catalytic mechanism of EIE. This study proposes a highly efficient and sustainable approach for the chemical modification of lipids, thereby promoting its broad application in the food industry.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109794"},"PeriodicalIF":3.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144088826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical isolation of breast cancer Circulating Tumor Cells with an inertial microfluidic chip with a trapezoidal cross-section 用梯形惯性微流控芯片临床分离乳腺癌循环肿瘤细胞

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-16 DOI: 10.1016/j.bej.2025.109788

Zahra Sadeghi , Mohsen Nasr Esfahany , Hossein Salehi , Azar Baradaran

{"title":"Clinical isolation of breast cancer Circulating Tumor Cells with an inertial microfluidic chip with a trapezoidal cross-section","authors":"Zahra Sadeghi , Mohsen Nasr Esfahany , Hossein Salehi , Azar Baradaran","doi":"10.1016/j.bej.2025.109788","DOIUrl":"10.1016/j.bej.2025.109788","url":null,"abstract":"<div><div>In recent years, various detection and isolation methods for breast cancer have emerged. However, the lack of simple, unbiased separation techniques limits clinical applications of Circulating Tumor Cells (CTCs). This study introduces an inertial microfluidic chip with a trapezoidal cross-section for rapid, simple separation of MCF-7 CTCs from patient blood using an optimized SLA 3D printing technique. Simulations and experiments showed that increasing the slant angle enhances Dean vortices, with 1.2 mL/min as the optimal flow rate. Spiking MCF-7 cells into Phosphate-Buffered Saline (PBS) yielded a recovery rate of 88 ± 3.5 % and a purity of 99.99 %. When spiked into 7.5 mL lysed blood, the recovery was 86 ± 2.9 % and purity 94 ± 2.3 %, with over 90 % of cells remaining viable. Clinically, the chip detected MCF-7 cells in 8 out of 10 breast cancer patients (80 %), with cell counts ranging from 2 to 21 cells/mL and purities between 89 ± 1.6 % and 92 ± 5.8 %. These results confirm the chip’s high sensitivity and viability preservation. This straightforward inertial microfluidic method offers a promising alternative to traditional affinity-based CTC separation, supporting fundamental research and aiding patient treatment strategies.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109788"},"PeriodicalIF":3.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shifts in microbial communities and antibiotic resistance genes in activated sludge driven by varying heterotrophic nitrifying aerobic denitrifying bacteria ratios 不同异养硝化好氧反硝化细菌比例驱动的活性污泥中微生物群落和抗生素抗性基因的变化

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-16 DOI: 10.1016/j.bej.2025.109790

Xiao-Yan Fan , Ji-Gang Ma , Wen-Nian Geng , Jia-Wei Ren , Shen-Bin Cao , Qing-Liang Sun , Jun-Ru Zhao

{"title":"Shifts in microbial communities and antibiotic resistance genes in activated sludge driven by varying heterotrophic nitrifying aerobic denitrifying bacteria ratios","authors":"Xiao-Yan Fan , Ji-Gang Ma , Wen-Nian Geng , Jia-Wei Ren , Shen-Bin Cao , Qing-Liang Sun , Jun-Ru Zhao","doi":"10.1016/j.bej.2025.109790","DOIUrl":"10.1016/j.bej.2025.109790","url":null,"abstract":"<div><div>Activated sludge systems experience reduced microbial activity under low-temperature conditions, which adversely affects the biological nitrogen removal process. Heterotrophic nitrifying aerobic denitrifying bacteria (HNADB), with their strong tolerance to low temperatures, present a promising solution through bioaugmentation. This study explored the bio-enhancement of activated sludge systems using HNADB to improve nitrogen removal, and evaluated microbial community structure, functional genes, and antibiotic resistance genes (ARGs) through batch experiments with varying inoculation ratios (from 0 % to 100 %) of HNADB-enriched sludge. Higher inoculation ratios (80 % and 100 %) significantly enhanced total nitrogen (TN) removal by 32.75 % and 43.62 %, respectively, though nitrification efficiency declined. Community diversity increased, while richness decreased under higher inoculation. Abundant taxa (AT) constituted the dominant proportion in all systems and were further enriched in the high-inoculation treatments. High inoculation ratios enriched denitrifying bacteria (DNB), HNADB (<em>Thauera</em>, <em>Hyphomicrobium</em>, <em>Rhodococcus</em>), and their related functional genes, while decreasing the abundance of nitrifying microorganisms and genes. The ARGs diversity increased but abundance decreased over time. DNB and HNADB may serve as hosts for ARGs, while nitrifying microorganisms show higher sensitivity to antibiotics. This study highlighted the potential of HNADB-based inoculation to enhance nitrogen removal performance and community resilience under low-temperature conditions, providing an effective biological enhancement strategy for low-temperature wastewater treatment.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109790"},"PeriodicalIF":3.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecularly-imprinted polymer based on graphene oxide functionalized pencil graphite electrode for cholesterol detection 基于氧化石墨烯功能化铅笔石墨电极的分子印迹聚合物用于胆固醇检测

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-16 DOI: 10.1016/j.bej.2025.109769

Fera Ayu Dianovita , Elly Septia Yulianti , Siti Hanafiah , Siti Fauziyah Rahman

{"title":"Molecularly-imprinted polymer based on graphene oxide functionalized pencil graphite electrode for cholesterol detection","authors":"Fera Ayu Dianovita , Elly Septia Yulianti , Siti Hanafiah , Siti Fauziyah Rahman","doi":"10.1016/j.bej.2025.109769","DOIUrl":"10.1016/j.bej.2025.109769","url":null,"abstract":"<div><div>Cholesterol plays a vital role in biological functions that must be maintained at a normal level of < 5.2 mmol/L to prevent hypercholesterolemia, leading to cardiovascular diseases. This research examines a molecularly-imprinted polymer (MIP) based biosensor using a pencil graphite electrode (PGE) modified graphene oxide (GO) for the sensitive, selective, and stable detection of cholesterol with a simple approach using the electropolymerization process. The imprinted poly[2-(dimethylamino) ethyl methacrylate] (poly[DMAEMA]) layers on PGE/GO surface exhibit strong electrochemical responses during cholesterol detection through the formation of cholesterol-specific recognition cavities that were formed to mimic the cholesterol’s structures, allowing non-enzymatic detection. Through the optimization of the parameters during electropolymerization, including the ratio of template molecule concentration to monomer, polymerization and template removal cycles, scan rate, rebinding duration, and pH, the sensor demonstrated satisfactory performance. With the limit of detection (LOD) of 0.85 mM, limit of quantification (LOQ) of 2.85 mM, a linear range of 1–6 mM, and sensitivity of 40.52 μA.μM⁻¹ .cm⁻², the sensor offered good electrochemical performance and selectivity towards cholesterol, despite the presence of other interference molecules. The current stability percentage on the tenth day was 85.52 %, which indicates good stability of the sensor modification and indicates potential for repeated cholesterol sensing for further point-of-care testing (POCT) application.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109769"},"PeriodicalIF":3.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144106669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal dynamics of algal-microbial interactions in a membrane photobioreactor for integrated nutrient remediation and microalgal resource recovery from shrimp aquaculture wastewater 膜光生物反应器中藻类与微生物相互作用的时空动态研究——用于对虾养殖废水营养物综合修复和微藻资源回收

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-16 DOI: 10.1016/j.bej.2025.109793

Tianyi Ye , Miaoxin Li , Derek M. Ayittey , Yuanyuan Qi , Zheng Sun , Zhijuan Jiang , Guoqi Zhang , Yun Ling

{"title":"Spatiotemporal dynamics of algal-microbial interactions in a membrane photobioreactor for integrated nutrient remediation and microalgal resource recovery from shrimp aquaculture wastewater","authors":"Tianyi Ye , Miaoxin Li , Derek M. Ayittey , Yuanyuan Qi , Zheng Sun , Zhijuan Jiang , Guoqi Zhang , Yun Ling","doi":"10.1016/j.bej.2025.109793","DOIUrl":"10.1016/j.bej.2025.109793","url":null,"abstract":"<div><div>The projected rise of global aquaculture to 106 million tons by 2030 has amplified concerns over nutrient-rich wastewater, demanding sustainable treatment solutions. This study developed an aerated membrane photobioreactor (MPBR) to simultaneously assess nutrient removal, biomass productivity, and algal–microbial community dynamics. High-throughput sequencing identified a dominant, stable consortium featuring the nitrogen-fixing <em>Azospirillum</em> (20.81 %) and the eukaryotic alga <em>Halochlorella</em> (53.19 %). Membrane surface analysis revealed a diverse biofilm community including <em>Limnothrix</em> (12.36 %) and <em>Scenedesmus</em> (28.70 %), suggesting synergistic microbial interactions. Isolated dominant species achieved total nitrogen (TN) removal of 58.36 %–65.50 %, total phosphate (TP) removal of 97.68 %–100 %, and ammonium (NH₄⁺-N) removal of 97.37 %–100 % at a 10-hour hydraulic retention time. These findings underscore the MPBR's dual functionality in effective nutrient remediation and microalgal biomass valorization, offering an informed strategy for resource recovery and environmentally sustainable aquaculture wastewater management.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109793"},"PeriodicalIF":3.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144154903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors influencing the removal of extracellular resistance genes in activated sludge process 活性污泥法去除胞外抗性基因的影响因素

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-15 DOI: 10.1016/j.bej.2025.109791

Jinyuan Xue , Mengqi Zhang , Anji Chen , Yuhan Li , Chenke Zhong , Chaoqi Chen

{"title":"Factors influencing the removal of extracellular resistance genes in activated sludge process","authors":"Jinyuan Xue , Mengqi Zhang , Anji Chen , Yuhan Li , Chenke Zhong , Chaoqi Chen","doi":"10.1016/j.bej.2025.109791","DOIUrl":"10.1016/j.bej.2025.109791","url":null,"abstract":"<div><div>Extracellular antibiotic resistance genes (eARGs) contribute to antimicrobial resistance spread in the environment, yet their removal mechanisms in activated sludge remain poorly understood. Here, an artificial resistant plasmid (PUC57-<em>sul</em>1) and its corresponding <em>sul</em>1 amplicon were treated in aerobic reactors under varying conditions. The removal of eARGs was significantly affected by sludge disinfection, biomass concentrations, aeration rate, and temperature. The results indicated that the influencing factors for eARGs are similar to those for small-molecular-organic compounds. At optimal conditions (5 g/L biomass concentration, 400 mL/min aeration rate, and 30°C), the removal efficiency at 12-h was 88.7 % for the amplicon and 67.4 % for the plasmid, respectively, indicating that eARG removal was largely affected by size and conformation. A transient increase of eARGs was observed when actual hospital wastewater was treated, likely due to the lysis of ARG-carrying bacteria. In undiluted hospital wastewater, the concentration of <em>sul</em>1 was reduced by 15.3 % relative to its peak concentration, even under the optimized conditions. In contrast, when the wastewater was 10-fold diluted, the concentration of <em>sul</em>1 was reduced by 81.3 %, confirming the effectiveness of the activated sludge process when toxicity was reduced. Microbial inhibition may be the key factor limiting eARG removal. These findings contribute to a better understanding of factors facilitating the degradation of eARGs, potentially guiding future strategies for improving wastewater treatment practices.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109791"},"PeriodicalIF":3.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing fatty acid production from waste activated sludge through low-temperature heat treatment and biostimulant pretreatment: Performance and mechanism analysis 通过低温热处理和生物刺激素预处理提高废活性污泥脂肪酸产量：性能及机理分析

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-12 DOI: 10.1016/j.bej.2025.109789

Yuan Shan , Yanyan Wang , Jing Zhang , Yihan Gong , Tiantian Yao , Yihao Wang , Guanghui Chen , Deshuang Yu

{"title":"Enhancing fatty acid production from waste activated sludge through low-temperature heat treatment and biostimulant pretreatment: Performance and mechanism analysis","authors":"Yuan Shan , Yanyan Wang , Jing Zhang , Yihan Gong , Tiantian Yao , Yihao Wang , Guanghui Chen , Deshuang Yu","doi":"10.1016/j.bej.2025.109789","DOIUrl":"10.1016/j.bej.2025.109789","url":null,"abstract":"<div><div>Anaerobic fermentation of sludge is constrained by low short-chain fatty acids (SCFAs) yields and limited solubilization of organic matter. This study presents a novel approach that integrates low-temperature heat treatment with biostimulant pretreatment to enhance the recovery of SCFAs from waste activated sludge. Experimental results indicate that applying 90 °C heat treatment and 1.2 g/g TSS D-limonene without pH adjustment led to an SCFAs yield of 2664 mg/L, representing a 2.3-fold increase over the control group. Furthermore, under conditions with initial pH set to 12, the SCFAs yield peaked at 2977 mg/L by the seventh day. Mechanistic analysis revealed that the combined pretreatment effectively disrupted extracellular polymeric substances and cell walls, facilitating SCFAs production. Microbial community analysis demonstrated an enrichment of Proteobacteria, Actinobacteria, and chain-elongating bacteria, enhancing the conversion of low-value organic matter into high-value products. The results offer new perspectives for optimizing anaerobic fermentation processes to enhance organic matter recovery from sludge.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109789"},"PeriodicalIF":3.7,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expressing and functionally evaluating anticancer bispecific antibody fragments using cyanobacterium Synechocystis sp. PCC6803 for sustainable production 利用聚胞蓝藻PCC6803表达抗癌双特异性抗体片段并对其进行功能评价

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-11 DOI: 10.1016/j.bej.2025.109776

Gota Kasai , Sayumi Shioya , Miku Kobe , Shunichi Kobayashi , Shouhei Takamatsu , Koki Makabe , Izumi Kumagai , Koji Sode , Ryutaro Asano

{"title":"Expressing and functionally evaluating anticancer bispecific antibody fragments using cyanobacterium Synechocystis sp. PCC6803 for sustainable production","authors":"Gota Kasai , Sayumi Shioya , Miku Kobe , Shunichi Kobayashi , Shouhei Takamatsu , Koki Makabe , Izumi Kumagai , Koji Sode , Ryutaro Asano","doi":"10.1016/j.bej.2025.109776","DOIUrl":"10.1016/j.bej.2025.109776","url":null,"abstract":"<div><div>Anticancer bispecific antibody (BsAb) fragments are effective therapeutic agents because of their high tissue penetration and cytotoxic effects, which can be produced in cost-effective bacterial hosts. We used the cyanobacterium <em>Synechocystis</em> sp. PCC6803 (PCC6803) as an alternative host for sustainably producing BsAb fragments from CO<sub>2</sub>. Cyanobacteria are sustainable and independent of external carbon sources such as glucose, because of producing substances via photosynthesis. Cyanobacteria can be cultured using wastewater or seawater, reducing freshwater consumption and the environmental impact of the process, suggesting that cyanobacteria can be used as a sustainable BsAb production platform. We examined the production of various antibody fragments, namely, single-chain bispecific diabody (scBsDb), single-chain fragment variable (scFv), variable domains of the heavy-chain antibody VHH, and bispecific tandem VHH (BsVHH), using PCC6803. scBsDb showed partial antigen-binding ability and no cytotoxic effects. In contrast, all scFvs and VHH had binding abilities and we prepared BsVHH up to 2.4 mg/L culture. BsVHH showed binding activity with the targets and cytotoxic effects against carcinoma, unlike scBsDb. Our results highlight the suitability of VHH and VHH-based engineered antibody fragments for using PCC6803 and indicate their potential for producing other therapeutic proteins. We report producing functional BsAb fragments using cyanobacteria. Our findings contribute to developing cyanobacterial bioprocesses for producing therapeutic BsAb fragments, expanding the possibilities for sustainably producing valuable materials from CO<sub>2</sub>.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109776"},"PeriodicalIF":3.7,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced biodesulfurization of recalcitrant organosulfur compounds by a newly identified Gordonia alkanivorans JT-2 in the presence of mycolic acid 在霉菌酸存在下，新鉴定的烷化戈登菌JT-2增强了难固定性有机硫化合物的生物脱硫作用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-11 DOI: 10.1016/j.bej.2025.109779

Maosheng Yin , Shuiquan Chen , Meng Zang , Lin Li , Chaocheng Zhao , Xiuxia Zhang

{"title":"Enhanced biodesulfurization of recalcitrant organosulfur compounds by a newly identified Gordonia alkanivorans JT-2 in the presence of mycolic acid","authors":"Maosheng Yin , Shuiquan Chen , Meng Zang , Lin Li , Chaocheng Zhao , Xiuxia Zhang","doi":"10.1016/j.bej.2025.109779","DOIUrl":"10.1016/j.bej.2025.109779","url":null,"abstract":"<div><div>With the increasing stringency of global regulations on the sulfur content of petroleum products, the development of efficient desulfurization technologies has become imperative. While biodesulfurization offers an eco-friendly alternative to hydrodesulfurization, its industrial adoption remains hindered by (i) poor degradation of alkylated DBTs/BTs and (ii) inefficient oil-water phase mass transfer. In this study, we identified a novel strain, <em>Gordonia alkanivorans JT-2</em>, demonstrating enhanced biodesulfurization performance, attributed to its production of mycolic acid, which improves hydrophobicity and mass transfer efficiency. Genomic analysis revealed key desulfurization genes (<em>dszA</em>, <em>dszB</em>, <em>dszC</em>, and <em>dszD</em>) and mycolic acid biosynthesis genes (<em>fas</em>, <em>pks13</em>, <em>kasAB</em>, <em>fabD</em>, and <em>fadD32</em>). Biodesulfurization experiments showed significant sulfur removal, reducing sulfur content in model oil to 35.7 ± 5.9 mg/L (DBT), 55.2 ± 7.9 mg/L (4-MDBT), 63.1 ± 5.7 mg/L (3-MBT), and 73.7 ± 3.8 mg/L (4,6-DMDBT) within 5 days. Protein interaction network analysis further illustrated the interplay between lipid biosynthesis and sulfur metabolism, underscoring the metabolic adaptability of strain JT-2. This work addresses a critical limitation in existing biodesulfurization technologies by improving mass transfer efficiency and broadening substrate range, positioning <em>G. alkanivorans</em> JT-2 as a promising candidate for industrial biodesulfurization applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109779"},"PeriodicalIF":3.7,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0