Biochemical Engineering Journal最新文献_第6页

Sustainable chitosan-egg white protein nanofiber membranes for efficient bacterial capture and killing 可持续的壳聚糖-蛋清蛋白纳米纤维膜，用于有效的细菌捕获和杀死

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-04-01 Epub Date: 2026-01-02 DOI: 10.1016/j.bej.2025.110067

Thi Tam An Tran , Edouard Gnoumou , Quang-Vinh Le , Nguyen The Duc Hanh , Nanthiya Hansupalak , Yi-Hua Gao , Bing-Lan Liu , Chi-Yun Wang , Kuei-Hsiang Chen , Yu-Kaung Chang

{"title":"Sustainable chitosan-egg white protein nanofiber membranes for efficient bacterial capture and killing","authors":"Thi Tam An Tran , Edouard Gnoumou , Quang-Vinh Le , Nguyen The Duc Hanh , Nanthiya Hansupalak , Yi-Hua Gao , Bing-Lan Liu , Chi-Yun Wang , Kuei-Hsiang Chen , Yu-Kaung Chang","doi":"10.1016/j.bej.2025.110067","DOIUrl":"10.1016/j.bej.2025.110067","url":null,"abstract":"<div><div>Egg whites are an abundant source of natural proteins with inherent biological activities, including antibacterial properties. In this study, a novel antibacterial nanofiber membrane was developed by immobilizing egg white proteins (EWP) onto an electrospun polyacrylonitrile (PAN) membrane. The PAN membrane was first modified via alkaline hydrolysis to introduce carboxyl groups (P-COOH), followed by chitosan (CS) grafting (P-COOH-CS) to enhance biocompatibility and protein attachment. EWP was then immobilized onto the surface through ionic and covalent interactions. The immobilization process was optimized by varying pH, CS molecular weight, and solute concentration. The resulting P-COOH-CS-EWP membrane exhibited strong antibacterial efficacy, achieving 98.25 ± 2.09 % reduction in Gram-negative <em>E. coli</em> and 88.24 ± 2.93 % reduction in Gram-positive <em>S. aureus.</em> Furthermore, the nanofiber membrane demonstrated good reusability and stability, retaining 75.84 ± 3.73 % activity after three cycles and 76.41 ± 0.79 % after four weeks of storage. This work highlights the potential of EWP-functionalized nanofiber membranes as a cost-effective, eco-friendly antibacterial material. The approach provides a sustainable strategy for recycling EWP waste and can be applied in biomedical, food packaging, and environmental applications that require antibacterial protection.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"228 ","pages":"Article 110067"},"PeriodicalIF":3.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing dry anaerobic digestion of pig manure via in-situ ammonia capture by adding magnesium salt-pretreated wheat straw 添加镁盐预处理麦秸原位捕氨强化猪粪干式厌氧消化

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-04-01 Epub Date: 2025-12-31 DOI: 10.1016/j.bej.2025.110064

Hongyuan Sun , Jiaqi Liu , Xuewen Gao , Kuizu Su , Rui Tang , Xinmin Zhan , Zhen-Hu Hu

{"title":"Enhancing dry anaerobic digestion of pig manure via in-situ ammonia capture by adding magnesium salt-pretreated wheat straw","authors":"Hongyuan Sun , Jiaqi Liu , Xuewen Gao , Kuizu Su , Rui Tang , Xinmin Zhan , Zhen-Hu Hu","doi":"10.1016/j.bej.2025.110064","DOIUrl":"10.1016/j.bej.2025.110064","url":null,"abstract":"<div><div>Dry anaerobic digestion (AD) is a promising technology for pig manure treatment, yet it is challenged by ammonia inhibition. Magnesium salt addition promotes the formation of magnesium ammonium phosphate (MAP), which <em>in-situ</em> captures ammonia nitrogen and mitigates inhibition, while high solids content limits MAP crystallization. This study evaluated the effect of magnesium salt pretreatment of wheat straw on dry AD of pig manure. Four Mg:P molar ratios (1.0:1.0, 1.5:1.0, 2.0:1.0, and 3.0:1.0) were investigated. Adding magnesium salt-pretreated wheat straw under molar ratios of 1.0:1.0 and 1.5:1.0 captured 14.3–14.4 % of ammonia nitrogen and reduced free ammonia by 19.0–19.1 %. Methane production increased by 15.0–19.2 % and substrate degradation by 8.4–8.6 %. Such pretreatment promoted MAP nucleation and growth on the straw surface, facilitating ammonia nitrogen capture. The capture of ammonia nitrogen restored the abundance of acetoclastic methanogens from 7.3 % to 12.5 %, thereby enhancing methane production. These results provide a practical strategy for mitigating ammonia inhibition in dry AD of pig manure.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"228 ","pages":"Article 110064"},"PeriodicalIF":3.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scale-up of a deep eutectic solvent-mediated Zr-MOF platform for sustainable production of rare ginsenoside CK via immobilized dual-enzyme catalysis 固定化双酶催化深度共晶溶剂介导Zr-MOF平台持续生产稀有人参皂苷CK的放大研究

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-04-01 Epub Date: 2026-01-04 DOI: 10.1016/j.bej.2025.110056

Yifan Liu, Qianqian Shen, Chunxiao Cui, Xiaojun Wang, Zhansheng Wu

{"title":"Scale-up of a deep eutectic solvent-mediated Zr-MOF platform for sustainable production of rare ginsenoside CK via immobilized dual-enzyme catalysis","authors":"Yifan Liu, Qianqian Shen, Chunxiao Cui, Xiaojun Wang, Zhansheng Wu","doi":"10.1016/j.bej.2025.110056","DOIUrl":"10.1016/j.bej.2025.110056","url":null,"abstract":"<div><div>Rare ginsenoside CK is in high demand due to its significant physiological activity, but its low natural abundance limits applications. While metal-organic framework (MOF)-based immobilized enzyme technology enables the conversion of ginsenoside Rb1 to CK, it remains largely confined to laboratory scale. In this study, a zirconium-based MOF (UiO-66-Ser) was greenly synthesized in a choline chloride-ethylene glycol deep eutectic solvent (DES) using <span>L</span>-serine as a modulator for the co-immobilization of snailase and β-glucosidase. This co-immobilized system achieved a CK yield of 88.89 % at the laboratory scale. To evaluate scalability, systematic investigations were conducted on the effects of scaling up the synthesis system (20–60 mL), cross-linking system (20–200 mL), and conversion system (1–100 mL). The results indicate that after large-scale production, immobilized enzymes consistently exhibit superior thermal stability, pH stability, and organic solvent stability compared to free enzymes. Even at the maximum scale (60 mL synthesis, 200 mL cross-linking), the CK yield remained around 65 % (65 ± 0.38 %). The conversion system achieved peak efficiency at 10 mL, with a CK yield reaching 90.41 %, and the immobilized enzyme showed a more gradual decline in yield than free enzymes during scale-up. This study confirms the excellent scalability of the DES-synthesized Zr-MOF-based dual-enzyme immobilization system, providing a theoretical foundation and practical support for the industrial application of efficient biotransformation of natural products.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"228 ","pages":"Article 110056"},"PeriodicalIF":3.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flocculation process performance evaluation of microalgae grown with different organic carbon sources and recycling of spent medium 不同有机碳源培养微藻絮凝工艺性能评价及废媒循环利用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-04-01 Epub Date: 2026-01-08 DOI: 10.1016/j.bej.2026.110076

Dian Dai , Li Feng , Qing Li , Sirui Lv , Zhe Zhang , Ruicheng Yang , Zhangfeng Hu , Liandong Zhu

{"title":"Flocculation process performance evaluation of microalgae grown with different organic carbon sources and recycling of spent medium","authors":"Dian Dai , Li Feng , Qing Li , Sirui Lv , Zhe Zhang , Ruicheng Yang , Zhangfeng Hu , Liandong Zhu","doi":"10.1016/j.bej.2026.110076","DOIUrl":"10.1016/j.bej.2026.110076","url":null,"abstract":"<div><div>Incorporating organic carbon sources into the growth medium serves as an effective method for promoting microalgal productivity. Importantly, variations in carbon metabolism significantly impact the efficiency of subsequent biomass harvesting processes. There is a clear necessity to evaluate and select carbon sources that enable large-scale microalgae growth while preserving the efficiency of subsequent biomass separation. In this study, three representative flocculants (alum, CPAM: cationic polyacrylamide, and CS: chitosan) were utilized to evaluate the flocculation performance of microalgae cultivated with diverse carbon sources. Furthermore, by monitoring fundamental physiological parameters of microalgae, the feasibility of spent medium following harvesting with different flocculants was systematically evaluated. The research findings indicated that the flocculation performance of sodium acetate‑cultured microalgae was inhibited when harvested with alum or CS, primarily due to excessive extracellular protein secretion. Ethanol can be considered the optimal carbon source choice. While markedly enhancing microalgal biomass, its use in conjunction with alum, CPAM, or CS enables efficient harvesting, outperforming other carbon sources. When microalgae were recultured in a solution containing 50 % recycled spent medium, minimal impact was observed on fundamental physiological indicators, with only a slight reduction in fatty acid unsaturation detected. This study provided valuable insights into the selection of appropriate organic carbon sources for promoting microalgal harvesting and clarified the spent medium after harvesting utilization strategies.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"228 ","pages":"Article 110076"},"PeriodicalIF":3.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-efficiency PHB production in Synechocystis salina M8 through sequential screening and optimization of bioprocess parameters salina Synechocystis M8高效产PHB生物工艺参数的筛选与优化

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-04-01 Epub Date: 2026-01-06 DOI: 10.1016/j.bej.2026.110073

Nguyen Thi Phuong Dung , Ta Thi Minh Anh , Bui Thi Thu Uyen , Luu Thi Thu Ha , Dao Duy Khanh , Tran Dang Thuan , Tran Huu Quang , Phan Quang Thang

{"title":"High-efficiency PHB production in Synechocystis salina M8 through sequential screening and optimization of bioprocess parameters","authors":"Nguyen Thi Phuong Dung , Ta Thi Minh Anh , Bui Thi Thu Uyen , Luu Thi Thu Ha , Dao Duy Khanh , Tran Dang Thuan , Tran Huu Quang , Phan Quang Thang","doi":"10.1016/j.bej.2026.110073","DOIUrl":"10.1016/j.bej.2026.110073","url":null,"abstract":"<div><div>Polyhydroxybutyrate (PHB) production by cyanobacteria represents a promising pathway toward net-zero CO₂ emissions and circular bioeconomy. This study evaluated a newly isolated strain, <em>Synechocystis salina</em> M8, to determine how key environmental factors regulate biomass accumulation and PHB synthesis. Using a Plackett–Burman design, light intensity (4500–13,500 lux), pH (5–9), and temperature (25–35 °C) were identified as the most influential parameters for biomass growth, while PHB accumulation was significantly affected only by pH. A subsequent Box–Behnken optimization defined the conditions that maximized strain performance: a light intensity of 9773 lux, pH 8.07, and temperature 31.97 °C. Under these optimized conditions, <em>S. salina</em> M8 achieved a high dry biomass concentration of 2.73 g L⁻¹ , with PHB content reaching 21.41 % of dry biomass, reflecting efficient intracellular biopolymer accumulation alongside robust growth. Beyond PHB production, the strain displayed strong environmental functionality, exhibiting substantial inorganic carbon assimilation (3.06–4.06 %) and remarkable nutrient removal efficiencies for phosphate (94.2–99.8 %) and nitrate (87.3–96.1 %). These attributes highlight its suitability for integrated CO₂ sequestration and wastewater bioremediation. Overall, the results demonstrate that precise environmental tuning is crucial for optimizing both biomass productivity and PHB yield. This study provides a practical framework for scaling cyanobacterial cultivation toward sustainable PHB production, supporting broader applications in circular bioeconomy development and climate mitigation strategies.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"228 ","pages":"Article 110073"},"PeriodicalIF":3.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced biodegradation of plastics through engineered two-stage anaerobic co-digestion: Integrating organic waste valorization 通过工程两阶段厌氧共消化增强塑料的生物降解：整合有机废物增值

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-04-01 Epub Date: 2026-01-16 DOI: 10.1016/j.bej.2026.110083

Penaganti Praveen, Debabrata Mazumder

{"title":"Enhanced biodegradation of plastics through engineered two-stage anaerobic co-digestion: Integrating organic waste valorization","authors":"Penaganti Praveen, Debabrata Mazumder","doi":"10.1016/j.bej.2026.110083","DOIUrl":"10.1016/j.bej.2026.110083","url":null,"abstract":"<div><div>The rapid increase in plastic production has led to a corresponding rise in plastic pollution, posing significant environmental challenges. The management of mixed plastic waste, which typically requires complex separation processes, could be simplified by co-digesting plastic waste with organic waste, such as fruit and vegetable waste, in an anaerobic digester. While some studies have explored the anaerobic digestion of plastics, the biodegradation rates are typically low, rendering it an inefficient solution for large-scale plastic waste treatment. To address this limitation, this study investigates the potential of a two-stage anaerobic co-digestion process with an experimental period of 330 days, emphasizing the role of hydrolytic bacteria, which are crucial for virgin plastic beads and plastic waste degradation. Results showed a reduction in methane production, ranging from 10.1 % to 39.4 % in the co-digestion batch compared to the control batch. However, the biodegradation rates were significantly higher than those observed in previous anaerobic digestion studies of plastic waste. Notably, the highest biodegradation rate was observed for polyvinyl chloride waste (21.01 %), while the lowest was for high-density polyethylene beads (13.02 %). Further confirmation of these findings was provided by SEM and FTIR analyses, which revealed distinct signs of plastic biodegradation. Additionally, microbial community analysis and carbon mass balance calculations were performed, further validating the enhanced biodegradability in this two-stage anaerobic digestion system.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"228 ","pages":"Article 110083"},"PeriodicalIF":3.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-cost solid-state fermentation of lipopeptides by Staphylococcus sp. DG-2 for petroleum-contaminated soil remediation 葡萄球菌DG-2低成本固态发酵脂肽修复石油污染土壤

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-04-01 Epub Date: 2026-01-08 DOI: 10.1016/j.bej.2026.110078

Quanli Man, Linhao Kang, Hanjie Zu, Zhineng Wu, Xiaodong Ma

{"title":"Low-cost solid-state fermentation of lipopeptides by Staphylococcus sp. DG-2 for petroleum-contaminated soil remediation","authors":"Quanli Man, Linhao Kang, Hanjie Zu, Zhineng Wu, Xiaodong Ma","doi":"10.1016/j.bej.2026.110078","DOIUrl":"10.1016/j.bej.2026.110078","url":null,"abstract":"<div><div>This study reports a novel petroleum-degrading strain of <em>Staphylococcus</em> sp. that addresses the challenges of high costs and low productivity in lipopeptide (LP) production. Strain DG-2 produces LP through solid-state fermentation using low-cost agricultural waste, specifically soybean meal. FTIR, HPLC-MS, and surface tension analyses confirmed the LP structure as CH<sub>3</sub>-(CH<sub>2</sub>)<sub>17</sub>-CHO-CH<sub>2</sub>-CO-Gly-Gly-Gly-Leu-Met-Leu-Leu, with a critical micelle concentration (CMC) of 160 mg/L, effectively reducing the surface tension to 26.9 mN/m while maintaining stability across a pH range of 8–12 and temperatures of 20–80°C. Under optimized SSF conditions (30°C, 6 d, 12.9 g soybean meal, 1.3 g/L MgSO<sub>4</sub>·7H<sub>2</sub>O, 0.38 g/L FeSO<sub>4</sub>), response surface methodology optimization achieved a remarkable LP concentration of 49.5 mg/g ds, representing a 25.8 % increase from initial conditions. Notably, DG-2 demonstrated 52.5 % degradation of C13-C26 alkanes and 71.1 % removal of 2–4 ring PAHs in crude oil. Under optimal washing conditions (0.2 g/L LP, water-soil ratio 10:1, 65°C, 60 min), a total petroleum hydrocarbon (TPH) removal of 53.0 % was achieved from heavily petroleum-contaminated soil (8.0 % TPH). These findings demonstrated that DG-2 is an exceptional candidate for petroleum bioremediation, offering both a high-performance microbial resource and an optimized low-cost production strategy with significant industrial potential.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"228 ","pages":"Article 110078"},"PeriodicalIF":3.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coupled biodegradable polymer composite and sulfur-driven mixotrophic denitrification toward municipal tailwater polishing: Process performance and microbial synergism 耦合可生物降解聚合物复合材料和硫驱动混合营养化反硝化用于城市尾水抛光：工艺性能和微生物协同作用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-04-01 Epub Date: 2026-01-02 DOI: 10.1016/j.bej.2025.110070

Qian-Dong Qin , Jun-Cheng Han , Jin Cai , Tong Cai , Hao-Nan Xiao , Hou-Yun Yang , Kan Wang

{"title":"Coupled biodegradable polymer composite and sulfur-driven mixotrophic denitrification toward municipal tailwater polishing: Process performance and microbial synergism","authors":"Qian-Dong Qin , Jun-Cheng Han , Jin Cai , Tong Cai , Hao-Nan Xiao , Hou-Yun Yang , Kan Wang","doi":"10.1016/j.bej.2025.110070","DOIUrl":"10.1016/j.bej.2025.110070","url":null,"abstract":"<div><div>To address the limited denitrification efficiency caused by low carbon-to-nitrogen (C/N) ratio in municipal wastewater effluents, a mixotrophic denitrification reactor (PWSMDR) was constructed utilizing polycaprolactone/wheat straw composite (PWS) and sulfur as dual electron donors. Its performance was systematically compared with a heterotrophic denitrification reactor (PWHDR) employing PWS as the sole electron donor. The results demonstrated that PWSMDR achieved a high nitrate removal efficiency of approximately 99.2 % under a hydraulic retention time (HRT) of 2 h, with a nitrate removal rate of 0.38 kg N/m<sup>3</sup>/d, representing an increase of nearly 23 % compared with PWHDR. Furthermore, PWSMDR exhibited enhanced resilience to shock loading. Autotrophic and heterotrophic denitrification pathways in PWSMDR were responsible for 33.6–62.3 % and 37.7–66.4 % of the nitrate removal, respectively. High-throughput sequencing further revealed a significant enrichment of key autotrophic denitrifiers (e.g. <em>Thiobacillus</em> and <em>Sulfurimonas</em>) and heterotrophic denitrifiers (e.g. <em>Thauera</em>, <em>Dechloromonas</em>, and <em>Diaphorobacter</em>) in PWSMDR. The abundance of key functional genes involved in carbon, sulfur, and nitrogen transformations was enhanced in PWSMDR, promoting more efficient nitrate reduction to N<sub>2</sub>. Additionally, the effluent COD and TN in PWSMDR were maintained at approximately 14 mg/L and 0.95 mg/L, respectively. These findings demonstrate that the PWS–sulfur mixotrophic denitrification strategy not only ensures highly efficient and stable nitrogen removal, but also offers a cost-effective and sustainable engineering approach for municipal tailwater polishing.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"228 ","pages":"Article 110070"},"PeriodicalIF":3.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanobody-drug conjugates as versatile tools for improving therapeutic potential 纳米-药物缀合物是提高治疗潜力的通用工具

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-04-01 Epub Date: 2026-01-02 DOI: 10.1016/j.bej.2026.110072

Sneha Banerjee, Anna Mariya, Sreeja Vangapally, Bhaskar Paidimuddala

{"title":"Nanobody-drug conjugates as versatile tools for improving therapeutic potential","authors":"Sneha Banerjee, Anna Mariya, Sreeja Vangapally, Bhaskar Paidimuddala","doi":"10.1016/j.bej.2026.110072","DOIUrl":"10.1016/j.bej.2026.110072","url":null,"abstract":"<div><div>Nanobody-Drug Conjugates (NDCs) represent a rapidly emerging class of targeted therapeutics that merge the precision of nanobodies with the potency of cytotoxic or functional drug payloads. Unlike traditional Antibody-Drug Conjugates (ADCs), NDCs offer superior advantages, such as improved tissue penetration, faster systemic clearance, and compatibility with modular engineering platforms. Despite these promising features, NDCs remain understated in clinical pipelines, emphasizing the need for integrated insights into their therapeutic development. This review provides an inclusive analysis of structural and functional optimization strategies for NDCs, including nanobody selection, site-specific conjugation chemistries, linker design, and payload engineering to enhance intracellular delivery and therapeutic index. This review also highlights unresolved challenges, including maintaining the biochemical stability of linkers under physiological conditions, the structural and functional integration of diverse drug payloads with nanobody scaffolds, and limited mechanistic insights into nanobody pharmacodynamics and fate following intracellular drug release. Furthermore, this review discusses the recent advancements in both preclinical models and early clinical investigations, with a focus on the expanding therapeutic utility of NDCs in oncology, infectious diseases, and molecular imaging applications to accelerate the clinical viability of NDCs as next-generation biologics.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"228 ","pages":"Article 110072"},"PeriodicalIF":3.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ready-to-use cryopreservation of undifferentiated induced pluripotent stem cells (iPSCs) without detachment from culture plates using D-proline and a synthetic polymer 使用d -脯氨酸和合成聚合物冷冻保存未分化的诱导多能干细胞（iPSCs），使其不脱离培养板

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2026-03-01 Epub Date: 2025-12-06 DOI: 10.1016/j.bej.2025.110041

Kenta Morita , Shinya Kawasaki , Tomoko Yashiro , Ryoko Futai , Chanhyon Kin , Aito Nakahashi , Hikaru Amo , Yukiya Kitayama , Takashi Aoi , Michiyo Koyanagi-Aoi , Tatsuo Maruyama

{"title":"Ready-to-use cryopreservation of undifferentiated induced pluripotent stem cells (iPSCs) without detachment from culture plates using D-proline and a synthetic polymer","authors":"Kenta Morita , Shinya Kawasaki , Tomoko Yashiro , Ryoko Futai , Chanhyon Kin , Aito Nakahashi , Hikaru Amo , Yukiya Kitayama , Takashi Aoi , Michiyo Koyanagi-Aoi , Tatsuo Maruyama","doi":"10.1016/j.bej.2025.110041","DOIUrl":"10.1016/j.bej.2025.110041","url":null,"abstract":"<div><div>Induced pluripotent stem cells (iPSCs) have been making a significant impact on the fields of regenerative medicine and cell biology. Several cell-freezing media for dispersed single-cell iPSCs are already commercially available. However, cryopreservation techniques for iPSCs cultured in 2D and 3D formats remain to be established. In this study, we developed a xeno-free cell-freezing medium containing <span>D</span>-proline and a synthetic block copolymer composed of 2-(dimethylamino)ethyl methacrylate (DEGMA) and 2-methacryloyloxyethyl phosphorylcholine (MPC), designated PDEGMA-<em>b</em>-PMPC-<em>b</em>-PDEGMA, that enables the cryopreservation of iPSCs cultured in 2D on microplates without detachment of the cells. Prior to cryopreservation, 2D-cultured iPSCs were treated with TrypLE Select Enzyme to weaken their adhesion to the microplate surfaces. Subsequently, the cells were cryopreserved in the cell-freezing medium containing <span>D</span>-proline and PDEGMA-<em>b</em>-PMPC-<em>b</em>-PDEGMA at −80°C for 2 days. At 48 h after thawing, the cell recovery (cell viability) was at least 70 % relative to the cell viability before freezing, while the cell recovery with commercially available media was 1.2 % at most. The most effective composition of the cell-freezing medium was 10 vol% DMSO, 5 vol% Dulbecco’s modified Eagle’s medium, 85 vol% water, 1 % (w/v) PDEGMA-<em>b</em>-PMPC-<em>b</em>-PDEGMA, 2 % (w/v) <span>D</span>-proline, and 0.35 % (w/v) NaCl. The cell recovery value remained stable after 3 months of cryopreservation. Most importantly, the iPSCs maintained their pluripotency after cryopreservation in the newly developed cell-freezing medium.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"227 ","pages":"Article 110041"},"PeriodicalIF":3.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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