Biochemical Engineering Journal最新文献_第6页

Carbon-based conductive carriers promote coupled Fe(II)-driven autotrophic and heterotrophic denitrification of wastewater with low C/N ratios 碳基导电载体促进低 C/N 比废水中铁(II)驱动的自养和异养耦合反硝化作用

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-08-22 DOI: 10.1016/j.bej.2024.109473

{"title":"Carbon-based conductive carriers promote coupled Fe(II)-driven autotrophic and heterotrophic denitrification of wastewater with low C/N ratios","authors":"","doi":"10.1016/j.bej.2024.109473","DOIUrl":"10.1016/j.bej.2024.109473","url":null,"abstract":"<div><p>Denitrification of wastewater with a low organic carbon to NO<sub>3</sub><sup>-</sup>-N ratio (C/N ratio) faces challenges due to slow rates and low efficiency. This study reported that carbon-based conductive carriers are able to enhance the removal of nitrogen from wastewater with low C/N ratio by coupling Fe(II)-driven autotrophic and heterotrophic bioelectrochemical denitrification. When Fe(II) was the sole electron donor, the bioreactor using conductive carrier achieved a denitrification rate constant (<em>k</em><sub><em>DN</em></sub>) of 0.016 h<sup>−1</sup>, 1.7 times of that with non-conductive materials. This enhancement was due to the conductive carrier boosting direct electron transfer and supporting the growth of electroactive microorganisms. For wastewater with a low C/N ratio of 0.76, the bioreactor featuring both Fe(II) and the conductive carrier reached a <em>k</em><sub><em>DN</em></sub> of 0.095 h<sup>−1</sup>, five times higher than without Fe(II). The presence of Fe(II) promoted denitrification by enhancing electron transfer and serving as a mediator. Microbial analysis showed that adding Fe(II) enriched electroactive bacteria like <em>Comamonas</em> and denitrifiers such as <em>Chryseobacterium</em>. Our findings suggest a promising strategy to enhance denitrification in wastewater treatment systems with low C/N ratios.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142076320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental study and mathematical modeling of the pectinases production by Aspergillus flavipes FP-500 in an airlift bioreactor 气升式生物反应器中黄曲霉 FP-500 生产果胶酶的实验研究和数学建模

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-08-22 DOI: 10.1016/j.bej.2024.109472

{"title":"Experimental study and mathematical modeling of the pectinases production by Aspergillus flavipes FP-500 in an airlift bioreactor","authors":"","doi":"10.1016/j.bej.2024.109472","DOIUrl":"10.1016/j.bej.2024.109472","url":null,"abstract":"<div><p>Mathematical models are indispensable for designing, optimizing, and controlling large-scale bioprocesses. In the present work, the production of pectinases was studied experimentally using an internal loop airlift bioreactor, pectin as a substrate, and <em>Aspergillus flavipes</em> FP-500 as a biocatalyst. The N-tanks-in-series (NTIS) model was implemented to predict the behavior of fungal growth, pectin and dissolved oxygen consumption, pectinases production, and the oxygen mass transfer rate from gas phase to liquid culture medium. A double <em>Monod-Logistic</em> kinetic model was used to describe the biomass growth rate as a function of biomass, pectin, and dissolved oxygen concentrations. In contrast, a <em>Luedeking-Piret kinetic model</em> was used to describe the production rate of endo and exo pectinases. A hydrodynamic model was utilized to estimate gas hold-ups, volumetric mass transfer coefficients, and air inflow velocities. Good agreement was observed between the experimental data and the theoretical results, demonstrating the predictive capacity of the NTIS model to describe pectinase production, the oxygen consumption rate, and the oxygen evolution in the gas phase. The model highlighted its robust capability to capture the critical parameters of aerobic fermentation processes. Therefore, it could be used as a tool for the scalability of the airlift bioreactors.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Macroparticle-enhanced morphology engineering of Cordyceps sinensis for high glucose fermentation to optimize the production of bioactive exopolysaccharides 用于高糖发酵的冬虫夏草大颗粒强化形态工程优化生物活性外多糖的生产

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-08-21 DOI: 10.1016/j.bej.2024.109470

{"title":"Macroparticle-enhanced morphology engineering of Cordyceps sinensis for high glucose fermentation to optimize the production of bioactive exopolysaccharides","authors":"","doi":"10.1016/j.bej.2024.109470","DOIUrl":"10.1016/j.bej.2024.109470","url":null,"abstract":"<div><p><em>Cordyceps sinensis</em> is widely known for its therapeutic properties. Enhancing the yield of exopolysaccharides (EPS), which is crucial for its medicinal efficacy, is a major challenge. In this study, we applied high initial glucose concentrations with talc particles to enhance EPS production and assessed the cell morphology, intracellular biochemical reactants, and bioactivity contribution of glycoproteins. The use of 150 g/L glucose and 10 g/L 2000 mesh talc increased the EPS yield by 1.8-fold to 4.21 g/L. The addition of talc regulated cell morphology, facilitated the entry of oxygen molecules into the cells to produce a large amount of ATP for polysaccharide synthesis, and altered the cell wall structure to facilitate the secretion of EPS. Moreover, environmental stress resulted in a notable increase in intracellular reactive oxygen species levels, which can potentially enhance cell membrane permeability and promote EPS synthesis. Furthermore, the highest protein content in crude EPS corresponded to the maximum activation of alcohol dehydrogenase (ADH) of 44.2 %, suggesting a mechanistic relationship between the proteins and polysaccharides in the glycoproteins that influence the activation of ADH. These findings elucidate the intricate interplay between fermentation conditions and EPS production and provide new avenues for optimizing the fermentation process of CS-HKI to enhance its therapeutic applications.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142020817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ozone/biological aerated filter integrated process for recycled paper mill wastewater: A pilot-scale study 臭氧/生物曝气滤池综合工艺处理回收造纸厂废水：试点规模研究

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-08-21 DOI: 10.1016/j.bej.2024.109466

{"title":"Ozone/biological aerated filter integrated process for recycled paper mill wastewater: A pilot-scale study","authors":"","doi":"10.1016/j.bej.2024.109466","DOIUrl":"10.1016/j.bej.2024.109466","url":null,"abstract":"<div><p>In this study, the effluent from recycled paper mill was treated using a combined ozone (O<sub>3</sub>) and biological aerated filter (BAF) process. Key operational parameters such as ozone dosage, pH, hydraulic retention time (HRT), volume load and gas-to-water ratio were optimized. Under optimal conditions, with a total ozone dosage of 100 g/m, a gas-to-water ratio of 4:1, and an HRT of 3.0 hours in the BAF, the chemical oxygen demand (COD) and chroma of the treated wastewater were reduced to 44–55 mg/L and 2–4 PCU, achieving removal efficiencies of 70 % and 95 %, respectively. The discharge effluent not only satisfy the new discharge standard of China (GB3544–2008), but also can be used as recycling water. Additionally, the treatment cost of wastewater was <em>ca.</em> 1.3 ¥/m<sup>3</sup> in pilot-scale test, significantly decreasing the cost. Ozone pretreatment has a significant effect on wastewater decolorization by disrupting the molecular chemical structure of pollutants, which increase the biochemical properties of biofilm and is beneficial to the sequential BAF treatment. The sludge in the O<sub>3</sub>/BAF system exhibited increased biomass with minimal filamentous bacteria and higher dehydrogenase activity, confirming stable and robust bacterial growth. GC-MS analysis revealed substantial reduction in pollutant content and diversity post-treatment, although the recalcitrant compound (<em>Z</em>)-13-docosenamide remained relatively high, decreasing from 27.37 % to 21.14 %. The mechanism of the O<sub>3</sub>/BAF process for the pollutant degradation were also proposed. This study demonstrated that a combination of ozone and fixed biofilm treatment is an efficient and cost-effective treatment, providing the theory and practical applicability for the industrial wastewater.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142040816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ubiquitous chromatin opening element enhance Darbepoetin alfa expression in CHO DG44 cell line 普遍存在的染色质开放元件可增强 Darbepoetin alfa 在 CHO DG44 细胞系中的表达

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-08-16 DOI: 10.1016/j.bej.2024.109468

{"title":"Ubiquitous chromatin opening element enhance Darbepoetin alfa expression in CHO DG44 cell line","authors":"","doi":"10.1016/j.bej.2024.109468","DOIUrl":"10.1016/j.bej.2024.109468","url":null,"abstract":"<div><p>Despite extensive efforts in biotherapeutics production, there is a pressing need to enhance the biomanufacturing process to meet the growing demand for recombinant products. Ubiquitous Chromatin Opening Element (UCOE), chromatin-modifying elements derived from CpG islands, are crucial in regulating gene expression. Therefore, to explore the UCOE effect on gene expression, we cloned codon-optimized Darbepoetin alfa (DPO) sequence into the pOptiVEC then the Darbepoetin alfa-IRES-DHFR fragment was digested, and cloned into the UCOE vector. Both pOptiVEC-DPO and UCOE-DPO-IRES-DHFR cassettes were linearized and transfected into the two cell pools of CHO DG44 cells. The transfected cells were adapted with CD OptiCHO™ medium after 20 days. Recombinant DPO expression was evaluated by qRT-PCR technique. Qualitative and quantitative protein expression analysis was also carried out by western blotting and ELISA assays. Our findings showed a boosted DPO expression rate at both mRNA and protein levels in the UCOE-DPO-IRES-DHFR pool compared to pOptiVEC-DPO. These data suggest that UCOE and codon optimization result in high and stable gene expression in the CHO DG44 cell line.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142011817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Core-shell bioactive capsule for aquaculture wastewater denitrification 用于水产养殖废水脱氮的核壳生物活性胶囊

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-08-16 DOI: 10.1016/j.bej.2024.109469

{"title":"Core-shell bioactive capsule for aquaculture wastewater denitrification","authors":"","doi":"10.1016/j.bej.2024.109469","DOIUrl":"10.1016/j.bej.2024.109469","url":null,"abstract":"<div><p>The lack of denitrifying bacteria and organic carbon sources, and the inhibition of dissolved oxygen (DO) result in nitrate accumulation in aquaculture wastewater. In order to solve this problem, an encapsulation method was introduced to prepare a novel bioactive capsule, which can provide organic carbon source, denitrifying bacteria, and anoxic microenvironment for aquaculture wastewater denitrification. And can reduce the recovery time of the enclosed denitrifying bacteria. The morphology of the capsule, its nitrate removal rate, and nitrogen conversion pathway in synthetic aquaculture wastewater were investigated. The capsule had a porous surface and the pore diameter ranged from 150.0 nm to 300.0 nm. The enclosed denitrifying bacteria had a reduced recovery time and excellent denitrification performance. The nitrate removal rate reached 86.2 % on the first day and was maintained at 99.7 %. Nitrogen conversion pathways in the capsule include denitrification, assimilatory/dissimilatory nitrate reduction, and nitrogen fixation. The denitrifying capsule has short recovery time and good denitrification performance, which would help to achieve denitrification in aquaculture wastewater or other low C/N wastewater.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142011816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of biochar-mediated reduction of antibiotic-resistant bacteria and biogas production enhancement in anaerobic digesters 生物炭在厌氧消化器中减少抗生素耐药菌和提高沼气产量的机理

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-08-13 DOI: 10.1016/j.bej.2024.109465

{"title":"Mechanisms of biochar-mediated reduction of antibiotic-resistant bacteria and biogas production enhancement in anaerobic digesters","authors":"","doi":"10.1016/j.bej.2024.109465","DOIUrl":"10.1016/j.bej.2024.109465","url":null,"abstract":"<div><p>This study examined the impact of different biochar (BC) as an anaerobic digestion (AD) additive on antibiotic-resistant bacteria (ARB) survival and AD performance using dairy cow manure. Bamboo BC and Olive BC with different particle sizes were added into the mesophilic AD at 15 g/L and 30 g/L dosages (Bamboo-15, Bamboo-30, Olive-15, and Olive-30). The study provides a detailed analysis of biogas production, organic metabolism, and ARB and microbial dynamics, elucidating the mechanisms by which BC influences AD. Findings reveal significant reductions in CEZ-resistant bacteria (CEZ-r) across all reactors, ranging from 12.88 % to 76.47 %. Both Bamboo and Olive BC increased CEZ-r removal by 3.08–5.94 times compared to the control. Additionally, BC supplementation prevented the rise in CEZ-r percentage within the total bacteria count observed in the control reactor. Bamboo BC outperformed Olive BC in enhancing biogas yield, with Bamboo-15 and Bamboo-30 showing significant increases of 43.2 % and 48.0 %, respectively, compared to the control. Adding BC in AD regulates ARB by decreasing potential ARG hosts and impeding the transmission of resistance. It also enhances biogas production by improving the efficiency of methanogenic bacteria and optimizing the methanogenic pathway. This research provides insights into how BC can be used to enhance AD performance and mitigate ARB proliferation, offering a sustainable approach to waste management and energy production.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrative metabolomics and metagenomics analysis of functional microbiota examines the extraction of bioactive products using longan pericarp 功能微生物群的代谢组学和元基因组学综合分析检验了利用龙眼果皮提取生物活性产品的过程

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-08-11 DOI: 10.1016/j.bej.2024.109464

{"title":"An integrative metabolomics and metagenomics analysis of functional microbiota examines the extraction of bioactive products using longan pericarp","authors":"","doi":"10.1016/j.bej.2024.109464","DOIUrl":"10.1016/j.bej.2024.109464","url":null,"abstract":"<div><p>Longan (<em>Dimocarpus longan</em> Lour.) is a nutrient-rich fruit, widely cultivated in China. However, its seeds and pericarp, often discarded as waste, are rich in bioactive components, particularly polyphenolics. In this research, we develop microbiota-assisted extraction technique, which is efficient and environmentally friendly for extracting these valuable components. These results showcased superior efficiency in the diversity and richness of extracted bioactive components, although yielding slightly lower TP content than ethanol extraction. The active components extracted by microbiota-assisted extraction technique increased to 2.45 mg/g, which was higher 2.13 times and 3.10 times compared to optimized ethanol and water extraction, respectively. The numbers of active components extracted by these three methods were 39, 24 and 30, separately, and Quercetin 3-D-xyloside and Kaempferol 3-O-alpha-L-rhamnopyranosyl were identified in longan pericarp extracts by microbiota-assisted extraction. Metagenomics analysis revealed stable and diverse microbiota with functional capabilities for enhancing extraction efficiency. Functional annotations indicated significant roles in biosynthesis, carbohydrate degradation, and fatty acid metabolism. Microbiota-assisted extracts exhibited a broad spectrum of bioactive compounds with potential health benefits, including antioxidant and anti-inflammatory properties. These results provide a theoretical foundation for developing sustainable extraction technologies.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141997454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A real-time oxygen uptake rate monitoring approach suitable for the antibody production process 适合抗体生产过程的实时氧气吸收率监测方法

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-08-10 DOI: 10.1016/j.bej.2024.109463

{"title":"A real-time oxygen uptake rate monitoring approach suitable for the antibody production process","authors":"","doi":"10.1016/j.bej.2024.109463","DOIUrl":"10.1016/j.bej.2024.109463","url":null,"abstract":"<div><p>Significant progress has been achieved in large-scale mammalian cell culture technology for biotherapeutics manufacturing over the past decades, necessitating the Process Analytical Technology (PAT) for the real-time measurement of critical quality attributes and the guidance for precise process control to ensure productivity, quality, and consistency. The Oxygen Uptake Rate (<span><math><mi>OUR</mi></math></span>) serves as a crucial indicator for characterizing the energy metabolism of mammalian cells, offering insights into cellular state and metabolism dynamics. However, current cellular <span><math><mi>OUR</mi></math></span> monitoring in antibody production depends mainly on costly gas analyzers or periodic manual sampling. Here, we introduce a novel method for in-line monitoring of cellular <span><math><mi>OUR</mi></math></span> in bioreactors based on the stationary liquid phase balance (SLPB) theory, which extends its applicability to diverse aeration and foam conditions without additional equipment or labor expenditures. We modeled the <span><math><mrow><msub><mrow><mi>k</mi></mrow><mrow><mi>L</mi></mrow></msub><mi>a</mi></mrow></math></span> of the aerated stirred bioreactor, assessed the influence of foam on liquid surfaces induced by gas sparging on oxygen transfer, and processed raw <span><math><mi>OUR</mi></math></span> data using a sliding filter. The established method was applied to monitoring the real-time <span><math><mi>OUR</mi></math></span> of Chinese Hamster Ovary (CHO) cell cultures for antibody production, demonstrating its excellent accuracy, sensitivity and readability. Aligned with the Quality by Design (QbD) concept, this real-time <span><math><mi>OUR</mi></math></span> estimation enables rapid detection of metabolic changes, revealing cellular physiology and facilitating precise feedback control in biotherapeutics manufacturing.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142007087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of the polishing process by integrating experimental design and high-throughput screening 通过整合实验设计和高通量筛选优化抛光工艺

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2024-08-10 DOI: 10.1016/j.bej.2024.109462

{"title":"Optimization of the polishing process by integrating experimental design and high-throughput screening","authors":"","doi":"10.1016/j.bej.2024.109462","DOIUrl":"10.1016/j.bej.2024.109462","url":null,"abstract":"<div><p>Complex bispecific antibody formats tend to form more product-related impurities than monoclonal antibodies. The primary constraints are yield and purity in the polishing stage. The purpose of this study was to enhance the understanding of the optimal working window for four mixed-mode resins and to reduce the burden of resin screening and parameter optimization during process development. This study optimized the loading and elution conditions of four different mixed-mode cationic resins to enhance the yield and purity by integrating Design of Experiments with High-Throughput Screening. It was observed that despite being weakly acidic mixed-mode cationic resins, these four resins exhibited significant differences in their adsorption and elution performances and varied tolerances to salt concentrations. Capto MMC demonstrated strong hydrophobicity, while the performance profiles of MX-Trp-650 M and Nuvia cPrime were similar, with Nuvia cPrime showing superior purification effects. Eshmuno CMX, by extending its side chain ligand, achieved higher binding efficiency and capacity. Through the optimization of salt concentrations or the application of dual-gradient elution strategies, the target protein with high yield (77 %) and purity over 99 % was successfully obtained. This research not only provides in-depth insights into the application of mixed-mode chromatography in the biopharmaceutical field but also offers practical optimization strategies for the industrial-scale purification of bispecific antibodies.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141978772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0