Biochemical Engineering Journal最新文献_第6页

Microfluidic filtration device for high throughput process development 用于高通量工艺开发的微流控过滤装置

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-23 DOI: 10.1016/j.bej.2025.109880

Nusrat Jahan , Jaanvi Gandhi , Nicolas Szita , Sheng-Ching Wang , Spyridon Konstantinidis , Marco PC Marques

{"title":"Microfluidic filtration device for high throughput process development","authors":"Nusrat Jahan , Jaanvi Gandhi , Nicolas Szita , Sheng-Ching Wang , Spyridon Konstantinidis , Marco PC Marques","doi":"10.1016/j.bej.2025.109880","DOIUrl":"10.1016/j.bej.2025.109880","url":null,"abstract":"<div><div>Sustainable biopharmaceutical manufacturing requires cost-effective, scalable, and efficient processes. To achieve this goal, access to scalable screening platforms providing rapid and high-quality data with low material requirements is required. While cell culture/fermentation and purification technologies meeting these criteria have been developed and deployed, microscale filtration solutions, which enable screening a wide range of filtration products and conditions, are largely absent. Microfluidic filtration devices are uniquely positioned to fill this technology gap since they can provide the necessary throughput for screening applications with low feed volumes and in-line monitoring. However, a lack of standardized approaches limits their industrial adoption. We present a microfluidic tangential flow filtration (μTFF) device that can accommodate any flat sheet-membrane. Membrane exchange is straightforward, enabling application in single-use or re-use formats. We successfully tested a wide flux range (30–1055 litres per square meter per hour, LMH), observed recovery yields exceeding 80 % in single-pass mode (1 bar transmembrane pressure, TMP), and demonstrated cleaning in place (CIP) procedures for extended membrane use with multiple filtration cycles (recoveries > 80 %). Furthermore, we integrated sensors to facilitate automation and generation of scale-relevant data, and provided criteria to facilitate pump selection. Our μTFF device offers, therefore, a standardised design paving the way for off-the-shelf microfluidic solutions for filtration optimisation, thus enhancing efficiency and effectiveness of bioprocess development.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109880"},"PeriodicalIF":3.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144723876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adsorption of Pb2+ and Cd2+ on silica-modified and nitrogen self-doped lotus leaf biochar: Adsorption behavior and mechanism 二氧化硅修饰和氮自掺杂荷叶生物炭对Pb2+和Cd2+的吸附：吸附行为和机理

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-19 DOI: 10.1016/j.bej.2025.109873

Yuanping Li , Suzhen Hou , Yaoning Chen , Yihuan Liu , Mengyang Zhao , Hongjuan Jiang , Nianping Chi , Guowen He , Shunyao jia

{"title":"Adsorption of Pb2+ and Cd2+ on silica-modified and nitrogen self-doped lotus leaf biochar: Adsorption behavior and mechanism","authors":"Yuanping Li , Suzhen Hou , Yaoning Chen , Yihuan Liu , Mengyang Zhao , Hongjuan Jiang , Nianping Chi , Guowen He , Shunyao jia","doi":"10.1016/j.bej.2025.109873","DOIUrl":"10.1016/j.bej.2025.109873","url":null,"abstract":"<div><div>Biochar and its modified products have received much attention in the adsorption treatment of heavy metal wastes, but there has been great potential for improvement in efficiency and cost. In this study, nitrogen-containing lotus leaves taken from lotus root harvests were pyrolyzed and modified by the silicon from industrial by-product micro-silicon powder, and then the Si-modified and nitrogen self-doped lotus leaf biochar (Si@N-BC) was prepared as an efficient material for the adsorption of Pb<sup>2+</sup> and Cd<sup>2+</sup> in aqueous solution. The results demonstrated that the adsorption performance of Si@N-BC was significantly enhanced than the pristine biochar, and the maximum adsorption capacities of Pb<sup>2+</sup> and Cd<sup>2+</sup> were estimated based on the Langmuir model to be 363.60 mg/g and 56.04 mg/g, respectively. The adsorption kinetic experiment indicated that the adsorption reaction was dominated by chemisorption. Moreover, the high performance of Si@N-BC is not only related to ion exchange and surface precipitation, but also the complexation reactions between oxygen-containing functional groups, nitrogen-containing functional groups, and silicon-containing functional groups. In conclusion, Si@N-BC is a novel and promising adsorbent that is conducive to the resourceful utilization of lotus leaf and micro-silicon powder, and its multiple functional groups can work together to achieve efficient removal of heavy metals from water.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109873"},"PeriodicalIF":3.7,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144694994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Species classification of microalgae using a CNN-based deep learning approach under optimal cultivation conditions 最优培养条件下基于cnn深度学习的微藻种类分类

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-19 DOI: 10.1016/j.bej.2025.109879

Gokce Kendirlioglu Simsek , Merve Ertargin , Laura Pezzolesi , Rossella Pistocchi , Ozal Yildirim , A. Kadri Cetin

{"title":"Species classification of microalgae using a CNN-based deep learning approach under optimal cultivation conditions","authors":"Gokce Kendirlioglu Simsek , Merve Ertargin , Laura Pezzolesi , Rossella Pistocchi , Ozal Yildirim , A. Kadri Cetin","doi":"10.1016/j.bej.2025.109879","DOIUrl":"10.1016/j.bej.2025.109879","url":null,"abstract":"<div><div>Microalgae possess significant potential in a wide range of applications due to their valuable bioactive compounds. They are utilized in biofuel production to reduce dependence on fossil fuels, in wastewater treatment for the biological removal of heavy metals and pollutants, in carbon capture for mitigating climate change, and in the pharmaceutical and nutraceutical industries as supplements. As their applications expand, the accurate and efficient identification of microalgal species becomes increasingly important. Traditional classification methods are time-consuming and rely heavily on expert knowledge. The main aim of this study is to develop a reliable, fast, and expert-independent deep learning-based approach for the classification of microalgae species using microscopic images. In this context, deep learning techniques, specifically convolutional neural network (CNN)-based models were employed to classify microalgal species. Four widely used pre-trained CNN architectures (ResNet152, DenseNet201, MobileNetV2, and EfficientNetB0), along with a custom-designed CNN, were implemented. The models were trained and tested on a labeled dataset consisting of microscopic images of Chlorella vulgaris, Scenedesmus acutus, and Haematococcus pluvialis. The classification models achieved accuracy rates ranging from 96.87 % (Custom CNN) to 100 % (DenseNet201), demonstrating the potential of CNN-based approaches in automating and improving microalgae species identification.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109879"},"PeriodicalIF":3.7,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144694995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial domestication and response of low C/N ammonia nitrogen wastewater treated by zonal influent micro-pressure internal circulation reactor 分区进水微压内循环反应器处理低碳氮比氨氮废水的微生物驯化及响应

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-17 DOI: 10.1016/j.bej.2025.109878

Fan Wang, Fangyuan Zhang, Wenai Liu, Yuying Fan, Kailun Yang, Jinhao Pu, Dejun Bian

{"title":"Microbial domestication and response of low C/N ammonia nitrogen wastewater treated by zonal influent micro-pressure internal circulation reactor","authors":"Fan Wang, Fangyuan Zhang, Wenai Liu, Yuying Fan, Kailun Yang, Jinhao Pu, Dejun Bian","doi":"10.1016/j.bej.2025.109878","DOIUrl":"10.1016/j.bej.2025.109878","url":null,"abstract":"<div><div>In this study, by distributing the influent in a micro-pressure internal circulation reactor (MPR) at a flow rate ratio of 1:2:1 to the anaerobic zone, anoxic zone, and aerobic zone, efficient denitrification of low C/N ammonia nitrogen wastewater was successfully achieved without the need for additional carbon sources or nitrified liquid reflux. Compared with the traditional treatment method of adding carbon sources, the treatment cost was reduced. By regulating the dissolved oxygen (DO) content in the growth environment of microbial flora, the nitrifying flora was first enriched, followed by the denitrifying and simultaneous nitrification-denitrification (SND) bacteria flora. During this process, the diversity of microbial populations decreased with the increase of DO concentration. The removal efficiency of COD, NH<sub>4</sub><sup>+</sup> -N and TN reached 95 %, 85 % and 80 % respectively. The 16S rRNA amplicon sequencing showed that the increase of dissolved oxygen during domestication promoted the proliferation of nitrifying bacteria, the relative abundance of nitrifying genes increased, while the secretion of extracellular polymers increased, and the activities of dehydrogenase activity and specific aerobic rate increased. Then, with the decrease of DO concentration, the relative abundance of nitrifying bacteria at the genus level wasn′t affected. However, the denitrifying functional bacteria increased significantly, especially the abundance of bacteria with SND functions, and the TN removal amount in the aerobic zone reached 21.24 % of the total removal amount.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109878"},"PeriodicalIF":3.7,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized solid composite bacterial agents for biodegradation of benzo[a]pyrene contaminated soils: Effects, microbial dynamic changes and mechanisms 优化固体复合菌剂对苯并[a]芘污染土壤的生物降解效果、微生物动力学变化及机制

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-16 DOI: 10.1016/j.bej.2025.109875

Bing Xiao, Jianli Jia, Xiaolong Gao, Mengyuan Zeng, Ben Zhang, Weiran Wang, Yichi Ma, Yuxin Han, Shuo Zhang

{"title":"Optimized solid composite bacterial agents for biodegradation of benzo[a]pyrene contaminated soils: Effects, microbial dynamic changes and mechanisms","authors":"Bing Xiao, Jianli Jia, Xiaolong Gao, Mengyuan Zeng, Ben Zhang, Weiran Wang, Yichi Ma, Yuxin Han, Shuo Zhang","doi":"10.1016/j.bej.2025.109875","DOIUrl":"10.1016/j.bej.2025.109875","url":null,"abstract":"<div><div>Solid composite bacterial agents (SCBA) present an innovative bioremediation strategy for the in situ degradation of benzo[<em>a</em>]pyrene (BaP), a recalcitrant compound that poses significant challenges to remediation in contaminated soils. In this study, an SCBA for BaP degradation was prepared using vacuum freeze-drying technology. Response surface methodology was employed to optimize the protective agent ratio, resulting in a composite formulation comprising 14 % skimmed milk powder, 8 % glycerol, and 8 % sucrose. This formulation achieved a freeze-drying survival rate of (89.65 %±2.04)% and an effective viable bacterial count of (1.50 ± 1.07)× 10 ¹ ¹ CFU/g in the SCBA. After 60 days of BaP-contaminated soil remediation, the BaP degradation rates in the activated bacterial liquid combined with co-metabolized substrate (CMS) (JYC) and the SCBA combined with CMS (JFC) groups were 57.23 % and 45.33 %, respectively, reflecting increases of 41.35 % and 29.45 % compared to the control (CK) group. Incorporating SCBA and CMS significantly enhanced the microbial BaP remediation efficiency and improved soil organic matter, alkaline nitrogen content, as well as fluorescein diacetate, catalase, and dehydrogenase activity. Furthermore, this approach enriched the population of beneficial bacteria involved in BaP degradation, fostering a more efficient and stable microbial network. Enhanced signaling between microorganisms facilitated faster material exchange and closer inter-bacterial contact. The CMS provided essential nutrients, enabling microorganisms to adapt more rapidly to their environment and reducing microbial remediation.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109875"},"PeriodicalIF":3.7,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering-driven precursor accumulation for enhanced biosynthesis of O-succinyl-L-homoserine via L-aspartate and L-homoserine pathway optimization 代谢工程驱动的前体积累，通过l-天冬氨酸和l-高丝氨酸途径优化促进o -琥珀酰- l-高丝氨酸的生物合成

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-15 DOI: 10.1016/j.bej.2025.109864

Jianmiao Xu , Linglong Huang , Yan Feng , Qilan Shan , Yuan Tao , Changqing Luo , Zhiqiang Liu , Yuguo Zheng

{"title":"Metabolic engineering-driven precursor accumulation for enhanced biosynthesis of O-succinyl-L-homoserine via L-aspartate and L-homoserine pathway optimization","authors":"Jianmiao Xu , Linglong Huang , Yan Feng , Qilan Shan , Yuan Tao , Changqing Luo , Zhiqiang Liu , Yuguo Zheng","doi":"10.1016/j.bej.2025.109864","DOIUrl":"10.1016/j.bej.2025.109864","url":null,"abstract":"<div><div>O-succinyl-L-homoserine (OSH) is a critical intermediate in L-homoserine synthesis and serves as a precursor for L-methionine, which is essential for various biosynthetic processes. This study employs <em>Escherichia coli</em> W3110 as the starting strain to enhance OSH production by eliminating competing and degrading pathways, while simultaneously promoting the accumulation of precursor substances. Initially, we optimized the phosphoenolpyruvate-pyruvate-oxaloacetate pathway through the overexpression of the genes <em>ppc</em>, <em>aspC</em>, <em>aspA</em>, and <em>gdhA*</em>, which significantly increased the supply of L-aspartate. In addition, we adjusted the promoters of the key enzyme genes, <em>metA</em>11 and <em>yjeH</em>, involved in the primary OSH synthesis pathway, resulting in the creation of strain OSH23. The engineered strain demonstrated notable improvements in OSH titer, yield, and productivity in a 5 L fermenter, achieving values of 131.99 g/L, 0.49 g/g, and 1.14 g/L/h, respectively. Further overexpression of <em>thrA</em><sup><em>fbr</em></sup> enhanced carbon flux, yielding strain OSH24 which produced 120.32 g/L OSH in a 5-L bioreactor with a glucose conversion of 0.51 g/g and productivity of 1.18 g/L/h. When the fermentation scale was scaled up to 50 liters, OSH24 achieved a final OSH concentration of 131.06 g/L, while maintaining a sugar acid conversion rate of 0.51 g/g. This study not only illustrates the successful application of advanced metabolic engineering for high-yield OSH production but also establishes a robust foundation for its industrial application in L-methionine biosynthesis.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109864"},"PeriodicalIF":3.7,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing the production of xylitol in recombinant Escherichia coli BL21 by metabolic engineering 利用代谢工程技术提高重组大肠杆菌BL21木糖醇的产量

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-15 DOI: 10.1016/j.bej.2025.109876

Peize Wang, Fuyao Guan, Min Wu, Chuyang Yan, Xin Xu, Haoju Wang, Qihang Chen, Wei Sun, Jianying Huang, Ping Yu

{"title":"Enhancing the production of xylitol in recombinant Escherichia coli BL21 by metabolic engineering","authors":"Peize Wang, Fuyao Guan, Min Wu, Chuyang Yan, Xin Xu, Haoju Wang, Qihang Chen, Wei Sun, Jianying Huang, Ping Yu","doi":"10.1016/j.bej.2025.109876","DOIUrl":"10.1016/j.bej.2025.109876","url":null,"abstract":"<div><div>Xylitol, a pentose sugar alcohol with sweetness comparable to sucrose, has advantageous properties such as low caloric value and anti-cariogenic characteristics, leading to its extensive applications in food, chemical, and pharmaceutical industries. Current industrial production predominantly employs chemical catalytic methods, which suffer from high cost and environmental concerns. In contrast, microbial fermentation through metabolic engineering enables direct conversion of xylose to xylitol via microbial metabolism, demonstrating superior advantages of cost-effectiveness, environmental friendliness, and mild reaction conditions. This study developed an engineered <em>Escherichia coli</em> strain for high-efficiency xylitol production by implementing three key strategies: 1) introducing the heterologous xylose reductase gene <em>xyrB</em>, 2) optimizing NADPH cofactor supply, and 3) CRISPR/Cas9-mediated knockout of the competing pathway gene <em>xylA</em>. Subsequent fermentation condition optimization achieved remarkable production performance. Under optimized conditions with 15 g/L xylose and 10 g/L glucose in a 1-L shake flask system, the engineered strain <em>E. coli</em> BL21(DE3)/<em>ΔptsGΔxylA</em>-pETDuet-1 -<em>xyrB</em>-<em>zwf</em> demonstrated maximum xylitol production of 13.8 g/L (0.92 g/g xylose), representing significant improvement in bioconversion efficiency. This study contributes to the industrial production of xylitol by engineered strain <em>E. coli</em> BL21(DE3) via metabolic engineering.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109876"},"PeriodicalIF":3.7,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144634178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KOH-impregnated oil shale biochar: Revealing mechanisms of Cu, Zn, Ni stabilization in sludge and mitigating leaching risks 氢氧化钾浸渍油页岩生物炭：揭示污泥中Cu、Zn、Ni稳定机制和降低浸出风险

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-14 DOI: 10.1016/j.bej.2025.109874

Diannan Huang , Hao Guan , Jinfan Song , Yulan Tang , Mingfu Bao , Dasong Xing , Liguo Miao , Tingting Li , Enbiao Wang

{"title":"KOH-impregnated oil shale biochar: Revealing mechanisms of Cu, Zn, Ni stabilization in sludge and mitigating leaching risks","authors":"Diannan Huang , Hao Guan , Jinfan Song , Yulan Tang , Mingfu Bao , Dasong Xing , Liguo Miao , Tingting Li , Enbiao Wang","doi":"10.1016/j.bej.2025.109874","DOIUrl":"10.1016/j.bej.2025.109874","url":null,"abstract":"<div><div>In sludge land application, secondary pollution from heavy metals remains a critical challenge. This study innovatively prepared biochar by pyrolyzing KOH-impregnated oil shale. Characterized by SEM,BET and FTIR, the obtained oil shale biochar (OS-BC) featured a porous structure with a specific surface area of 64.3226 m<sup>2</sup>/g. The effects OS-BC dosage (5 %∼40 %) and reaction time (0–20 days) on the speciation transformation of Cu, Zn, and Ni in sludge were investigated. Results showed that optimal stabilization occurred at 30 % OS-BC dosage with 10-day reaction, achieving conversion rates of 37.29 %, 35.07 %, and 46.72 % for Cu, Zn, and Ni from labile to stable fractions. Visual MINTEQ simulation and XRD analysis revealed that OS-BC increased concentrations of oxygenated ions (OH⁻, CO<sub>3</sub><sup>2-</sup>), promoting the formation of insoluble hydroxide/carbonate precipitates and stable minerals. Simulated rainfall tests showed that cumulative leaching concentrations of Cu, Zn, and Ni were reduced by 60.3 %, 80.7 %, and 79.3 % compared to the untreated group, confirming enhanced environmental safety. This study provides a novel approach to the synergistic resource utilization of oil shale and sludge.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109874"},"PeriodicalIF":3.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144634176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of heat and chloramphenicol pretreatments on sludge anaerobic bioconversion: Insights into physicochemical and microbial disruption 热和氯霉素预处理对污泥厌氧生物转化的影响：对物理化学和微生物破坏的见解

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-13 DOI: 10.1016/j.bej.2025.109872

Anam Jalil , Hikmatullah Ahmadi , Chengyu Zhang , Xiangyang Wang , Zhisheng Yu

{"title":"Impact of heat and chloramphenicol pretreatments on sludge anaerobic bioconversion: Insights into physicochemical and microbial disruption","authors":"Anam Jalil , Hikmatullah Ahmadi , Chengyu Zhang , Xiangyang Wang , Zhisheng Yu","doi":"10.1016/j.bej.2025.109872","DOIUrl":"10.1016/j.bej.2025.109872","url":null,"abstract":"<div><div>This study examines impact of heat and chloramphenicol pretreatments on sludge characteristics to improve anaerobic bioconversion under mesophilic conditions. Physicochemical, microbial, and biogas analyses were conducted to evaluate the effect of each pretreatment. Elemental composition analysis revealed that chloramphenicol pretreatment reduced the C/N ratio to 12.8 ± 0.18, whereas heat treatment maintained a more balanced ratio of 15.0 ± 0.12, compared to 13.83 in the control. Macromolecular analysis revealed extensive microbial lysis in chloramphenicol-treated sludge, reflected by significant increases in protein (43.5 ± 0.6 %) and lipid (12.0 ± 0.5 %) contents, whereas moderate changes were observed in heat-pretreated samples. The lowest VS/TS ratio (0.60 ± 0.02) in the chloramphenicol group indicated enhanced organic solubility. Biogas assays revealed that heat-pretreated sludge produced the highest cumulative yield (35.0 mL/g VS) and CH₄ content (61 %), while chloramphenicol treatment yielded slightly less gas (33.0 mL/g VS) but with an elevated H₂ content (18 %), higher than that of the control (20.4 mL/g VS). COD and TVFA reductions of 20.0 % and 77.8 %, respectively, were also notable under chloramphenicol stress. Microbial analysis revealed an enrichment of spore-forming <em>Firmicutes</em> following heat pretreatment and a hydrogenogenic shift toward <em>Chloroflexi</em> and <em>Actinobacteriota</em> under antibiotic stress. Overall, these findings demonstrated how targeted pretreatment alters sludge composition and microbial ecology to optimize biogas quality, particularly by enhancing hydrogen production for sustainable bioenergy recovery.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"223 ","pages":"Article 109872"},"PeriodicalIF":3.7,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sludge-derived biochar promoted the methane production in anaerobic digestion of thermo-alkaline pretreated waste-activated sludge: Performance and mechanisms 污泥源生物炭促进热碱性预处理污泥厌氧消化产甲烷：性能与机理

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-07-10 DOI: 10.1016/j.bej.2025.109861

Yin-Ping Hou , Fang-Yuan Wang , Wang-Tao Dong , Hong-Rui Ma , Bin-Bin Cai

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