Biochemical Engineering Journal最新文献_第7页

Degradation of malachite green by a novel salt-tolerant Vibrio natriegens: Optimal degradation conditions, mechanisms, and safety assessment 一种新型耐盐营养弧菌降解孔雀石绿：最佳降解条件、机制和安全性评估

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-11 DOI: 10.1016/j.bej.2025.109775

Ruihong Sun , Qingzhong Wang , Shuqin Shao , Shanshan Zhang , Ying Wang

{"title":"Degradation of malachite green by a novel salt-tolerant Vibrio natriegens: Optimal degradation conditions, mechanisms, and safety assessment","authors":"Ruihong Sun , Qingzhong Wang , Shuqin Shao , Shanshan Zhang , Ying Wang","doi":"10.1016/j.bej.2025.109775","DOIUrl":"10.1016/j.bej.2025.109775","url":null,"abstract":"<div><div>Synthetic dyes, such as malachite green (MG), are widely used in the textile industry but pose significant environmental risks due to their toxicity. In this study, a highly efficient salt-tolerant bacterial strain, <em>Vibrio natriegens</em> SWS5, was isolated and identified through morphological, physiological, biochemical, and 16S rRNA gene sequence analyses. Systematic investigations were conducted to optimize degradation conditions, analyze enzyme activities, elucidate degradation pathways, and assess detoxification effects. The optimal degradation conditions were determined as follows: peptone 7.5 g/L, NaCl 20 g/L, Fe²⁺ 0.04 g/L, pH 7, temperature 30 °C, and shaking speed 220 rpm. Under these conditions, SWS5 achieved 97.88 % degradation of MG (100 mg/L) within 168 hours. After MG added, enzyme activity assays revealed in dye-decolorizing peroxidase (823 U/L), laccase (358 U/L), and manganese peroxidase (0.204 U/L) activities. HPLC-MS analysis identified key intermediates, enabling the proposal of two potential MG degradation pathways. Toxicity assessments using microbial tests and zebrafish models demonstrated a significant reduction in the toxicity of degradation products. Transcriptome sequencing provided novel insights into the regulatory pathways of MG degradation, offering a theoretical foundation for the bioremediation of MG contaminated wastewater. <em>Vibrio natriegens</em> was demonstrated for the first time to degrade triphenylmethane dyes, especially MG, in high-salinity environments. This study highlights the potential of <em>Vibrio natriegens</em> SWS5 as an efficient and eco-friendly solution for dye wastewater treatment.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109775"},"PeriodicalIF":3.7,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impacts of the combined application of digestate-based compost and biochar on the heavy metal Cd distribution and stress in the plant-soil system 沼液堆肥与生物炭配施对植物-土壤系统重金属Cd分布及胁迫的影响

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-08 DOI: 10.1016/j.bej.2025.109773

Feng Zhen , Hengbing Zhou , Linpei Han , Yifan Zhang , Yuwan Pang , Tao Xing , Lei Li

{"title":"Impacts of the combined application of digestate-based compost and biochar on the heavy metal Cd distribution and stress in the plant-soil system","authors":"Feng Zhen , Hengbing Zhou , Linpei Han , Yifan Zhang , Yuwan Pang , Tao Xing , Lei Li","doi":"10.1016/j.bej.2025.109773","DOIUrl":"10.1016/j.bej.2025.109773","url":null,"abstract":"<div><div>In this study, a pot experiment was conducted to evaluate the effects of digestate-based compost, chemical fertilizer, and their combination with biochar on heavy metal distribution, plant growth, and soil microbial communities. The experimental results demonstrated that the combined application of digestate-based compost and biochar (OFB group) promoted heavy metal immobilization in soil and enhanced plant growth, while reducing the accumulation of heavy metals in plants. Oxidizable and residual Cd levels in the OFB group increased by 14.90 % and 17.60 % compared to the control group. The biomass of Chinese cabbage was 384 % higher than that of the control group, and its Cd content decreased by 57.90 % compared to the control group. The addition of digestate-based compost to the soil increased soil organic matter, total nitrogen, total phosphorus, total potassium, and electrical conductivity (p < 0.05), along with the relative abundance of beneficial microorganisms such as <em>Bacillus</em>, <em>norank_f__norank_o__Actinomarinales</em>, <em>Chryseolinea</em>, <em>Saccharomonospora</em>, <em>Pseudomonas,</em> and <em>norank_f__BIrii41</em>. Biochar played a significant role in minimizing the migration of heavy metals to plants. The study demonstrated that the combined application of digestate-based compost and biochar reduces heavy metal stress, enhances soil fertility, and promotes biomass growth, offering potential solutions for the sustainable management of cadmium-contaminated soil and the application of digestate-based compost.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109773"},"PeriodicalIF":3.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A constructive bottom-up approach for the elaboration of metabolic networks: Case study of photosynthetic cyanobacteria Arthrospira spirulina platensis PCC 8005

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-08 DOI: 10.1016/j.bej.2025.109770

Maxime Maton , Baptiste Leroy , Alain Vande Wouwer

{"title":"A constructive bottom-up approach for the elaboration of metabolic networks: Case study of photosynthetic cyanobacteria Arthrospira spirulina platensis PCC 8005","authors":"Maxime Maton , Baptiste Leroy , Alain Vande Wouwer","doi":"10.1016/j.bej.2025.109770","DOIUrl":"10.1016/j.bej.2025.109770","url":null,"abstract":"<div><div>Mathematical modeling has proven to be a highly effective tool for understanding microbial metabolism for which in-silico and experimental studies help to quantify intracellular mechanisms and pave the way for optimizing the production of molecules of interest. In that context, the development of metabolic networks turns out to be particularly interesting despite the challenges underlying their reconstruction. While the elaboration of genome-scale networks is computationally costly, small networks are often oversimplified and important biological mechanisms might be omitted, which limits their use in industrial applications. For this purpose, this study proposes a constructive bottom-up approach for the identification of metabolic networks of intermediate size, typically comprised of a couple of hundred reactions. It combines basic biological knowledge and a series of constraint-based methods in an iterative strategy, enabling the refinement of the network definition. The network is first validated using in-silico data, and subsequently refined using experimental data to enhance its biological relevance. Several case studies have been addressed to assess the efficiency of the methodology, and this paper focuses on the modeling of photosynthetic cyanobacteria <em>Arthrospira</em> sp. PCC 8005. The procedure is effective and provides promising results and metabolic analyses show consistent predictive capabilities of the network, in concordance with existing studies.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109770"},"PeriodicalIF":3.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to: “Responses of nitrogen removal, microbial community and antibiotic resistance genes to biodegradable microplastics during biological wastewater treatment” [Biochem. Eng. J. 219 (2025) 109732] 《废水生物处理过程中氮去除、微生物群落和抗生素耐药基因对可生物降解微塑料的反应》[生物化学]。Eng。J. 219 (2025) 109732]

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-08 DOI: 10.1016/j.bej.2025.109774

Yiwei Zhou , Shaochen Bian , Hua Wang , Yixuan Chu , Lei Zheng , Yali Song , Chengran Fang

引用次数: 0

Development of an artificial biosynthetic pathway for biosynthesis of cinnamyl cinnamate in engineered Escherichia coli 工程大肠杆菌合成肉桂酸酯的人工生物合成途径的建立

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-08 DOI: 10.1016/j.bej.2025.109771

Jin Yang , Qiuli Wang , Hong Pan , Daoyi Guo

{"title":"Development of an artificial biosynthetic pathway for biosynthesis of cinnamyl cinnamate in engineered Escherichia coli","authors":"Jin Yang , Qiuli Wang , Hong Pan , Daoyi Guo","doi":"10.1016/j.bej.2025.109771","DOIUrl":"10.1016/j.bej.2025.109771","url":null,"abstract":"<div><div>Cinnamyl cinnamate is a key flavoring and bioactive compound present in various plants. It is used in a variety of products including fragrances for decorative cosmetics, fine fragrances, shampoos, toilet soaps, and other toiletries, as well as in non-cosmetic items such as household cleaners and detergents. The production of cinnamyl cinnamate through plant extraction and chemical synthesis is inefficient and environmentally unfriendly. Therefore, a promising and attractive alternative is the production of cinnamyl cinnamate from renewable carbon sources using microbial cell factories. In this study, the construction of a <em>de novo</em> cinnamyl cinnamate pathway in <em>Escherichia coli</em> has been demonstrated for the first time. Subsequently, by increasing the supply of precursor substrates, we have further improved the biosynthesis of cinnamyl cinnamate. Finally, by knocking out the <em>tyrA</em> gene to block the competitive pathway, the cinnamyl cinnamate production was increased to 769 mg/L. It represents a sustainable and environmentally friendly alternative for the synthesis of cinnamyl cinnamate.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109771"},"PeriodicalIF":3.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing synthetic biology for sustainable industrial innovation: Advances, challenges, and future direction 利用合成生物学促进可持续工业创新：进展、挑战和未来方向

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-08 DOI: 10.1016/j.bej.2025.109777

Emmanuel Chimeh Ezeako , Barine Innocent Nwiloh , Malachy Chigozie Odo , Vincent E. Ozougwu

{"title":"Harnessing synthetic biology for sustainable industrial innovation: Advances, challenges, and future direction","authors":"Emmanuel Chimeh Ezeako , Barine Innocent Nwiloh , Malachy Chigozie Odo , Vincent E. Ozougwu","doi":"10.1016/j.bej.2025.109777","DOIUrl":"10.1016/j.bej.2025.109777","url":null,"abstract":"<div><div>Synthetic biology (Synbiology) is an emerging science that leverages the genetic engineering of biological systems to accomplish industrial operations and produce valuable and desired products. Synbiology-inspired fabrication and reprogramming of biological systems has opened avenues for unearthing critical questions in chemical and biological engineering and harnessing the production of value-added chemicals, including biotherapeutics, food items, biocosmetics, biopolymers, and biofuels. However, engineering biological systems still lags far behind designing physical systems in precision, scalability, and market viability. Tackling these limitations is essential to unlocking the full industrial potential of Synbiology, from molecular compound selection to large-scale commercial production. This review x-rays the innovative contributions of Synbiology to industrial operations, emphasizing its role in advancing sustainable production of high-value chemicals, enhancing industrial efficiency, optimizing biofuel production, and enabling carbon capture and utilization. The findings highlight the pivotal role of Synbiology-based technology in driving a bio-based economy and fostering a sustainable future while identifying technical bottlenecks that must be addressed to elevate bioengineering practices to match other engineering disciplines.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109777"},"PeriodicalIF":3.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyvinylpyrrolidone-assisted in situ enzymes encapsulation within ZIF-8 for enhanced glycerol conversion 聚乙烯吡咯烷酮辅助原位酶包封在ZIF-8内，以增强甘油转化

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-08 DOI: 10.1016/j.bej.2025.109778

Linsong Luo , Xiao Han , Xiaoyan Dong , Qinghong Shi , Yan Sun

{"title":"Polyvinylpyrrolidone-assisted in situ enzymes encapsulation within ZIF-8 for enhanced glycerol conversion","authors":"Linsong Luo , Xiao Han , Xiaoyan Dong , Qinghong Shi , Yan Sun","doi":"10.1016/j.bej.2025.109778","DOIUrl":"10.1016/j.bej.2025.109778","url":null,"abstract":"<div><div>The growth of biodiesel and oleochemical industries results in substantial surpluses of glycerol and consistently low prices. To absorb this surplus, we report a polymer-assisted <em>in situ</em> enzymes encapsulation strategy in which glycerol dehydrogenase (GDH) is encapsulated with polyvinylpyrrolidone (PVP) and cysteine (Cys) within ZIF-8 for glycerol conversion. The results showed that the encapsulated GDH/ZIF-8 composites presented stronger substrate affinities and higher catalytic efficiencies than free GDH as well as good stability. Among the free GDH and GDH/ZIF-8 composites, GDH/Cys/PVP@ZIF-8 presented the highest relative activity of 300.7 %. This could be attributed to the structural evolution of the encapsulated GDH and the formation of a more hydrophilic microenvironment around GDH during complexation with PVP. To increase cofactor utilization, a cofactor self-sufficient cascade system was constructed by encapsulating GDH and forming H<sub>2</sub>O NADH oxidase (Nox) with PVP and Cys within ZIF-8 for the conversion of glycerol to 1,3-dihydroxyacetone (DHA). At the optimal GDH:Nox molar ratio (4:1) and a substrate concentration of 100 mM, the DHA yield reached approximately 4.2 mM in GDH-Nox/Cys/PVP@ZIF-8, and the total turnover number of GDH-Nox/Cys/PVP@ZIF-8 reached 2787 at 0.05 µM NAD<sup>+</sup>, indicating its feasibility for large-scale DHA production. This work has thus provided a promising procedure of co-immobilizing enzymes via Cys/PVP-assisted <em>in situ</em> encapsulation within ZIF-8 for the development of economically viable multienzyme biocatalysts for glycerol conversion.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"221 ","pages":"Article 109778"},"PeriodicalIF":3.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anodic modification-enhanced microbial electrolysis cell coupled with anaerobic digestion for coal gasification wastewater treatment 阳极改性强化微生物电解池耦合厌氧消化处理煤气化废水

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-07 DOI: 10.1016/j.bej.2025.109767

Yajie Li , Yuyao Zhang , Ou Wang , Weikang Kong , Salma Tabassum

{"title":"Anodic modification-enhanced microbial electrolysis cell coupled with anaerobic digestion for coal gasification wastewater treatment","authors":"Yajie Li , Yuyao Zhang , Ou Wang , Weikang Kong , Salma Tabassum","doi":"10.1016/j.bej.2025.109767","DOIUrl":"10.1016/j.bej.2025.109767","url":null,"abstract":"<div><div>The treatment of coal gasification wastewater (CGW) by anodic modification enhanced microbial electrolysis cell coupled with anaerobic digestion was discussed. In this study, iron sulfate was used to load modified biochar to obtain anode modified materials. The results showed that the electrode modification effectively improved the specific surface area of the graphite felt electrode, The conductivity of modified Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub> activated carbon was 191.4 ± 0.16 μS/cm, Fe-O-C functional groups boost electrode surface electron transfer. Three reactors were set up as the experimental groups: the anaerobic (AD) reactor (R1) as the control group, the microbial electrolysis cell coupled anaerobic digestion (MEC-AD) reactor (R2) and, the anode modified microbial electrolysis cell coupled anaerobic digestion (MEC-AD) reactor (R3). The results showed that R1, R2 and R3 had average removal rates of total phenol by 41 %, 48 % and 67 %, respectively. The degradation trend of quinoline and indole in R1, R2 and R3 was similar to that of total phenol. The degradation rates of quinoline and indole in R3 were the highest, reaching 86 % and 89 %, respectively. The electrode modification is beneficial to improve the treatment effect of MEC-AD. In addition, electrode modification promoted the enrichment of electroactive microorganisms such as <em>Syntrophus</em> and <em>Pseudomonas</em>, which was conducive to promoting direct interspecies electron transfer (DIET) and enhancing the degradation of organic pollutants.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109767"},"PeriodicalIF":3.7,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering robust β-glucuronidase via protein assembly and immobilization for enhanced glycyrrhizin hydrolysis 工程稳健的β-葡萄糖醛酸酶，通过蛋白质组装和固定化，以增强甘草酸水解

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-07 DOI: 10.1016/j.bej.2025.109772

Qibin Wang , Jing Yang , Weijie Cao , Hu Liu , Chun Li

引用次数: 0

N-octanoyl-DL-homoserine lactone-mediated quorum sensing enhances microbial degradation of petroleum hydrocarbons in saline-alkali soils n -辛烷酰dl -同丝氨酸内酯介导的群体感应增强了盐碱地中石油烃的微生物降解

IF 3.7 3区生物学

Biochemical Engineering Journal Pub Date : 2025-05-03 DOI: 10.1016/j.bej.2025.109768

Lin Li , Jiahua Li , Xiaoying Yu , Bo Wei , Yizhan Liu , Jingyi Zhu , Huaji Sun , Gang Zhou

{"title":"N-octanoyl-DL-homoserine lactone-mediated quorum sensing enhances microbial degradation of petroleum hydrocarbons in saline-alkali soils","authors":"Lin Li , Jiahua Li , Xiaoying Yu , Bo Wei , Yizhan Liu , Jingyi Zhu , Huaji Sun , Gang Zhou","doi":"10.1016/j.bej.2025.109768","DOIUrl":"10.1016/j.bej.2025.109768","url":null,"abstract":"<div><div>Bioremediation of petroleum-contaminated soil is limited by the inherent capacity of indigenous microorganisms to metabolize petroleum hydrocarbons. Microbial proliferation could stimulate the biodegradation of pollutants. This study investigated the ability of exogenous N-octanoyl-DL-homoserine lactone (C8-HSL) to improve hydrocarbon degradation through quorum sensing (QS) regulation. <em>Pseudomonas Stutzeri</em> M3 was inoculated into petroleum-contaminated soil to elucidate the regulatory mechanisms of C8-HSL-mediated QS on several key factors of petroleum degradation. Specifically, it affects soil respiration activity, the activity of key degradation enzymes, and the role of extracellular polymeric substances (EPS). The findings demonstrate that adding 100 nM C8-HSL significantly increased the degradation rate of petroleum hydrocarbons in soil by 37.83 % and markedly abbreviated the overall bioremediation time. Concurrently, exogenous acyl-homoserine lactone (AHL) has been shown to augment the quantity and diversify the composition of EPS in the system. The interaction between 100 nM C8-HSL and predominant degrading bacteria (<em>Pseudomonas</em>) facilitates the enrichment of petroleum hydrocarbon-degrading microbial populations. This study elucidates the QS-driven mechanisms underlying efficient total petroleum hydrocarbon (TPH) degradation in soil matrices, demonstrating the pivotal role of C8-HSL in stimulating indigenous microorganisms for oilfield bioremediation. Our findings provide novel mechanistic insights into AHL-mediated QS regulation of microbial petroleum degradation. The petroleum-contaminated soil remediation technology has dual advantages in treatment efficiency and economic benefits, and it is economically and practically significant.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109768"},"PeriodicalIF":3.7,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0