Development of an artificial biosynthetic pathway for biosynthesis of cinnamyl cinnamate in engineered Escherichia coli

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2025-05-08 DOI:10.1016/j.bej.2025.109771

Jin Yang , Qiuli Wang , Hong Pan , Daoyi Guo

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引用次数: 0

Abstract

Cinnamyl cinnamate is a key flavoring and bioactive compound present in various plants. It is used in a variety of products including fragrances for decorative cosmetics, fine fragrances, shampoos, toilet soaps, and other toiletries, as well as in non-cosmetic items such as household cleaners and detergents. The production of cinnamyl cinnamate through plant extraction and chemical synthesis is inefficient and environmentally unfriendly. Therefore, a promising and attractive alternative is the production of cinnamyl cinnamate from renewable carbon sources using microbial cell factories. In this study, the construction of a de novo cinnamyl cinnamate pathway in Escherichia coli has been demonstrated for the first time. Subsequently, by increasing the supply of precursor substrates, we have further improved the biosynthesis of cinnamyl cinnamate. Finally, by knocking out the tyrA gene to block the competitive pathway, the cinnamyl cinnamate production was increased to 769 mg/L. It represents a sustainable and environmentally friendly alternative for the synthesis of cinnamyl cinnamate.

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工程大肠杆菌合成肉桂酸酯的人工生物合成途径的建立

肉桂酸酯是存在于多种植物中的一种重要的香料和生物活性化合物。它用于各种产品，包括用于装饰化妆品的香水，精细香水，洗发水，香皂和其他洗漱用品，以及非化妆品产品，如家用清洁剂和洗涤剂。通过植物提取和化学合成生产肉桂酸酯效率低，对环境不友好。因此，利用微生物细胞工厂从可再生碳源中生产肉桂酸酯是一种很有前途和吸引力的替代方法。在这项研究中，首次证实了在大肠杆菌中构建一个新的肉桂酰肉桂酸途径。随后，通过增加前体底物的供应，我们进一步改善了肉桂酸的生物合成。最后，通过敲除tyrA基因阻断竞争通路，使肉桂酸酯的产量增加到769 mg/L。它代表了一种可持续和环保的替代肉桂酰肉桂酸酯的合成。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.