Enrichment of microbial consortium and performance evaluation for single cell protein production in hydrogen-mediated microbial electrosynthesis reactors

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2025-08-13 DOI:10.1016/j.bej.2025.109901

Jinglei Tian , Xiaoying Shen , Yonggang Liu , Xueqi Wang , Jinzhe Liu , Yonglong Jin , Hong Zhang , Kai Cui , Kun Guo

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Abstract

The exponential expansion of the global population has raised substantial concerns regarding potential food security crises in the foreseeable future, compounded by environmental degradation and greenhouse gas emissions associated with conventional agricultural practices. Fortunately, edible microbial protein has emerged as a promising sustainable alternative. Thereinto, hydrogen-oxidizing bacteria (HOB) coupled with microbial electrosynthesis (MES) technology have garnered considerable attention for their potential in sustainable protein production. This investigation focused on enriching HOB compatible with electrochemical systems through H₂-mediated MES, utilizing ammonium as the nitrogen source. Following a 65-day cultivation period in a sleeve-type bioreactor, taxonomic analysis revealed that Ancylobacter, identified as the predominant HOB genus, constituted 86.89 % of the microbial community. Furthermore, through optimization of current operational parameters, we achieved a significant enhancement in biomass productivity of autotrophic HOB. The biomass yield reached 121 ± 1 mg/L/day, representing a 46 % increase compared to the initial enrichment rate. The protein content was quantified at 64 ± 2 %, demonstrating parity with bacterial and fish meal proteins, while substantially exceeding that of soybean meal.

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氢介导微生物电合成反应器中微生物群落的富集及单细胞蛋白质生产性能评价

全球人口的指数级增长引起了人们对可预见的未来可能出现的粮食安全危机的严重关切，而与传统农业做法有关的环境退化和温室气体排放又加剧了这一问题。幸运的是，食用微生物蛋白已经成为一种有前途的可持续替代品。其中，氧化氢细菌（HOB）与微生物电合成（MES）技术的结合在可持续蛋白质生产方面的潜力引起了人们的广泛关注。本研究以氨为氮源，通过h2介导的MES富集与电化学体系相容的HOB。在套式生物反应器中培养65 d后，分类分析表明，钩虫属（anylobacter）为优势HOB属，占微生物群落的86.89% %。此外，通过优化当前操作参数，我们实现了自养HOB生物量生产力的显著提高。生物量产量达到121 ± 1 mg/L/天，比初始富集率提高了46 %。蛋白质含量为64 ± 2 %，与细菌和鱼粉蛋白质含量相当，大大超过豆粕蛋白质含量。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.