Developing an efficient open-pond microalgae cultivation system for producing biomass and fucoxanthin using Pavlova granifera IML2238

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2025-04-22 DOI:10.1016/j.bej.2025.109762

Chun-Yen Chen , Yu-Han Chang , Yoong Kit Leong , Jo-Shu Chang

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引用次数: 0

Abstract

Fucoxanthin is a marine carotenoid recognized for its strong antioxidant and free radical scavenging properties. However, most research on microalgae-based fucoxanthin production has been limited to laboratory scales (typically under 200 liters), providing limited insights for commercial applications. Therefore, there is an urgent need to establish scaled-up fucoxanthin-producing systems. In this study, a 6-ton open-pond system was employed to produce fucoxanthin using a fucoxanthin-rich microalgal strain Pavlova granifera IML2238. With an inoculum size of 0.10 g/L, batch cultivation achieved biomass and fucoxanthin productivity of 74.9 and 3.24 mg/L/d, respectively. A semi-batch process, replacing 50 % of the medium volume in each cycle, resulted in biomass and fucoxanthin productivity of 80.2 and 4.31 mg/L/d, respectively. Repeated semi-batch replacement at 50 % maintained stable biomass and fucoxanthin productivity at 73.7 and 4.05 mg/L/d. The fucoxanthin produced demonstrated high antioxidant activity, with a total phenolic content of 29.8 mg GAE/g, confirming its potential for commercial use.

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利用花叶巴甫洛夫（Pavlova granifera） IML2238开发生产生物质和岩藻黄素的高效开放池塘微藻培养系统

岩藻黄素是一种海洋类胡萝卜素，以其强大的抗氧化和自由基清除特性而闻名。然而，大多数基于微藻的岩藻黄质生产研究仅限于实验室规模（通常低于200升），对商业应用的见解有限。因此，迫切需要建立规模化的岩藻黄素生产系统。本研究采用6吨露天池塘系统，利用富含岩藻黄素的微藻菌株Pavlova granifera IML2238生产岩藻黄素。当接种量为0.10 g/L时，批量培养的生物量和岩藻黄素产量分别为74.9和3.24 mg/L/d。采用半间歇工艺，每次循环替换50% %的培养基体积，生物量和岩藻黄质产量分别为80.2和4.31 mg/L/d。以50% %的比例重复更换半批，生物量和岩藻黄质产量稳定在73.7和4.05 mg/L/d。所制得的岩藻黄质具有较高的抗氧化活性，总酚含量为29.8 mg GAE/g，具有一定的商业应用潜力。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.