Artificial Cells, Nanomedicine, and Biotechnology最新文献_第6页

A novel PDT: 5-aminolevulinic acid combined 450 nm blue laser photodynamic therapy significantly promotes cell death of HR-HPV infected cells. 新型PDT:5-氨基乙酰丙酸联合450 nm蓝色激光光动力疗法显著促进HR-HPV感染细胞的细胞死亡。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2023-12-01 DOI: 10.1080/21691401.2022.2164585

Yuqing Chen, Yibo Mei, Lijiang Gu, Xing Li, Peng Guo, Lihong Chen, Dalin He

{"title":"A novel PDT: 5-aminolevulinic acid combined 450 nm blue laser photodynamic therapy significantly promotes cell death of HR-HPV infected cells.","authors":"Yuqing Chen, Yibo Mei, Lijiang Gu, Xing Li, Peng Guo, Lihong Chen, Dalin He","doi":"10.1080/21691401.2022.2164585","DOIUrl":"10.1080/21691401.2022.2164585","url":null,"abstract":"Human papillomavirus (HPV) infection and related diseases are clinical challenges. The efficacy of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) using red laser (630 ± 5 nm) is remarkable and safe. In this study, we aim to investigate the efficacy of ALA-450 nm PDT comparing with ALA-635 nm PDT. We detected cell proliferation and cell apoptosis through MTT assay and flow cytometry assay respectively. Flow cytometry assay determined the intracellular reactive oxygen species (ROS) generation. Western blotting analysis investigated the protein expression. In vivo, immunohistochemical staining assay and TUNEL assay were performer to detect cell apoptosis. ALA-450 nm PDT inhibited the proliferation of End1 and HeLa cells, promoted cell apoptosis more effectively than ALA-635 nm PDT, and induced cell death probably through increasing the intracellular ROS generation and caspase-dependent apoptosis pathway. In vivo, ALA-450 nm PDT significantly inhibited tumour growth and activated cell apoptosis. The ALA-450 nm PDT had an advantage over ALA-635 nm PDT on inhibiting the proliferation of End1 and HeLa cells and inducing cell apoptosis. The ALA-450 nm PDT might be a promising therapeutic strategy for eradicating the HR-HPV infected cells and promoting the integration of diagnosis and treatment of HR-HPV related diseases.HighlightsWe combined 5-aminolevulinic acid with 450 nm blue laser using as a novel type of photodynamic therapy.The ALA-450 nm PDT had an advantage over ALA-635 nm PDT on inhibition of the proliferation of End1 and HeLa cells and inducing cell apoptosis in vitro and in vivo.The ALA-450 nm PDT may provide a novel alternative therapeutic option in patients with persistent HPV infection and promote the integration of diagnosis and treatment.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"51 1","pages":"22-32"},"PeriodicalIF":5.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10528911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound-triggered release of miR-199a-3p from liposome nanobubbles for enhanced hepatocellular carcinoma treatment. 超声触发miR-199a-3p从脂质体纳米气泡中释放用于增强肝细胞癌治疗。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2023-12-01 Epub Date: 2023-10-18 DOI: 10.1080/21691401.2023.2268137

Xinmin Guo, Jianru Lin, Liwen Pan, Kun He, Zhihui Huang, Jialin Chen, Cuiyan Lin, Baohui Zeng, Sijia Luo, Mengdie Wang

{"title":"Ultrasound-triggered release of miR-199a-3p from liposome nanobubbles for enhanced hepatocellular carcinoma treatment.","authors":"Xinmin Guo, Jianru Lin, Liwen Pan, Kun He, Zhihui Huang, Jialin Chen, Cuiyan Lin, Baohui Zeng, Sijia Luo, Mengdie Wang","doi":"10.1080/21691401.2023.2268137","DOIUrl":"10.1080/21691401.2023.2268137","url":null,"abstract":"This study was aimed to develop an efficient tumour-targeted liposome nanobubbles (LNBs) system using ultrasound-targeted nanobubble destruction for enhanced release and transfection of miRNA-199a-3p in hepatocellular carcinoma (HCC) therapy. The prepared LNBs comprised a polyethylene glycol-modified liposome shell and a perfluoropentane (PFP) core. MiRNA-199a-3p was attached to the nanocomposite surface via electrostatic adsorption, while RGD peptide functionalized the LNBs surface for enhanced HCC cell targeting, namely PFP@miR-RGD-LNBs. The LNBs were spherical with a narrow size distribution. The gene-loaded LNBs effectively condensed miR-199a-3p and protected it from enzymatic degradation. Low-intensity focused ultrasound (LIFU) promoted a fast release of miR-199a-3p from the prepared LNBs, thereby enhancing therapeutic effects. The combined application of PFP@miR-RGD-LNBs and LIFU exhibited a more potent inhibitory effect on HepG2 cells than the other groups, potentially due to LIFU promoting rapid and efficient gene release at the target site and increasing cell membrane permeability. Quantitative reverse transcription-polymerase chain reaction analysis revealed significantly increased mRNA expression levels of key apoptosis markers (Bad, Bax, Caspase-9 and Caspase-3) in the PFP@miR-RGD-LNBs + LIFU group compared to other groups. These findings suggest that the prepared LNBs are highly likely to be promising candidates for further exploration of HCC gene delivery and therapy.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"51 1","pages":"560-571"},"PeriodicalIF":5.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statement of retraction: Anti-gastric cancer effect of Salidroside through elevating miR-99a expression. 撤回声明：柳苷通过提高miR-99a的表达对癌症的抗先锋作用。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2023-12-01 Epub Date: 2023-10-23 DOI: 10.1080/21691401.2023.2271702

引用次数: 0

A study on the therapeutic potential of graphene titanate nanocomposite for treating chemically induced arthritis in rats. 钛酸石墨烯纳米复合材料治疗化学诱导的大鼠关节炎的潜力研究。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2023-12-01 Epub Date: 2023-10-30 DOI: 10.1080/21691401.2023.2268653

Amany Belal, Mohamed Y Zaky, Doaa S Mohamed, Eman E Mohamed, Rehab Mahmoud, Doaa Essam, R R Atta, Fatma I Abo El-Ela, Fatma Mohamed Halfaya, Kyung-Tae Lee, Ahmed H E Hassan, Mohammed M Ghoneim, Ahmed Farghali

{"title":"A study on the therapeutic potential of graphene titanate nanocomposite for treating chemically induced arthritis in rats.","authors":"Amany Belal, Mohamed Y Zaky, Doaa S Mohamed, Eman E Mohamed, Rehab Mahmoud, Doaa Essam, R R Atta, Fatma I Abo El-Ela, Fatma Mohamed Halfaya, Kyung-Tae Lee, Ahmed H E Hassan, Mohammed M Ghoneim, Ahmed Farghali","doi":"10.1080/21691401.2023.2268653","DOIUrl":"https://doi.org/10.1080/21691401.2023.2268653","url":null,"abstract":"Nanotechnology holds substantial promise in the innovative therapies for rheumatoid arthritis (RA). The current study was designed to synthesize and characterize a new graphene titanate nanocomposite (GTNc) and explore its anti-arthritic, anti-inflammatory, and antioxidant potencies against Complete Freund's adjuvant (CFA)-induced arthritis in rats, as well as investigate the underlying molecular mechanisms. Our characterization methods included XRD, FT-IR, SEM, EDX, zeta potential, practical size, and XRF to characterize the novel GTNc. Our findings revealed that arthritic rats treated with GTNc exhibited lower levels of RF, CRP, IL-1β, TNF-α, IL-17, and ADAMTS-5, and higher levels of IL-4 and TIMP-3. In arthritic rats, GTNc reduced LPO levels while increasing GSH content and GST antioxidant activity. Additionally, GTNc decreased the expression of the TGF-β mRNA gene in arthritic rats. Histopathological investigation showed that GTNc reduced inflammatory cell infiltration, cartilage degradation, and bone destruction in joint injuries caused by CFA in the arthritic rats. Collectively, the anti-arthritic, anti-inflammatory, and antioxidant properties of GTNc appear promising for future arthritis treatments and bone disability research.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"51 1","pages":"590-603"},"PeriodicalIF":5.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71410426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in screening and diagnostic lab-on-chip tools for gynaecological cancers - a review. 妇科癌症筛查和诊断芯片实验室工具的进展——综述。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2023-12-01 Epub Date: 2023-11-07 DOI: 10.1080/21691401.2023.2274047

Sadeka Nujhat, Hannah S Leese, Mirella Di Lorenzo, Rebecca Bowen, Sandhya Moise

引用次数: 0

Statement of retraction: Mirt2 functions in synergy with miR-377 to participate in inflammatory pathophysiology of Sjögren's syndrome. 撤回声明：Mirt2与miR-377协同作用，参与干燥综合征的炎症病理生理学。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2023-12-01 Epub Date: 2023-10-23 DOI: 10.1080/21691401.2023.2271700

引用次数: 0

Cancer reduction in mice with Prakasine nanomedicine immunotherapy. 用普拉卡辛纳米药物免疫疗法降低小鼠癌症。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2023-12-01 Epub Date: 2023-10-26 DOI: 10.1080/21691401.2023.2270023

Prakash S K

{"title":"Cancer reduction in mice with Prakasine nanomedicine immunotherapy.","authors":"Prakash S K","doi":"10.1080/21691401.2023.2270023","DOIUrl":"10.1080/21691401.2023.2270023","url":null,"abstract":"In this study, non-toxic mercury nanoparticle Prakasine (PRK-NP) was synthesized as per 'Prakash theory of metal drugs' and nanoparticle's non toxicity has been demonstrated by employing in vitro MTT (dose = 320ug/ml), SBR (dose = 80ug/ml) and apoptosis assays (dose = 320ug/ml), and in vivo acute and chronic toxicity studies in mice (n = 12, dose = 900 mg/kg body weight oral), rat (n = 14, dose = 500 mg/kg body weight oral for 18 months), rabbit (n = 14, dose = 500 mg/kg body weight oral for 18 months) and dogs (n = 14, dose = 500 mg/kg body weight oral for 18 months). The MTT, SBR and apoptosis assays established no cytotoxicity, no genotoxicity and no cytolytic anticancer effects. The mice, rat, rabbit and dog studies also indicated nontoxicity. The PRK-NPs significantly reduced the breast cancer tumour in murine mammary tumour - C3H/HeJ model 35% and 43.7% in mice at doses of 200 mg/kg and 500 mg/kg respectively. Also, in xenograft mammary tumour mice model the tumour regressions are 25.7% and 83% in the doses of 500 mg/kg and 1000 mg/kg respectively, compared to standard positive control drugs without any adverse effects and toxicity. Thus, the current study beholds anticipation PRK-NPs may play a vital role in therapeutic.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"51 1","pages":"572-589"},"PeriodicalIF":5.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50160528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Process-structure-biofunctional paradigm in cellular structured implants: an overview and perspective on the synergy between additive manufacturing, bio-mechanical behaviour and biological functions. 细胞结构植入物的过程结构-生物功能范式：增材制造、生物机械行为和生物功能之间协同作用的概述和展望。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2023-12-01 Epub Date: 2023-11-07 DOI: 10.1080/21691401.2023.2278156

R D K Misra, K P Misra

{"title":"Process-structure-biofunctional paradigm in cellular structured implants: an overview and perspective on the synergy between additive manufacturing, bio-mechanical behaviour and biological functions.","authors":"R D K Misra, K P Misra","doi":"10.1080/21691401.2023.2278156","DOIUrl":"https://doi.org/10.1080/21691401.2023.2278156","url":null,"abstract":"The overview describes the synergy between biological sciences and cellular structures processed by additive manufacturing to elucidate the significance of cellular structured implants in eliminating stress shielding and in meeting the bio-mechanical property requirements of elastic modulus, impact resistance, and fatigue strength in conjunction with the biological functionality. The convergence of additive manufacturing, computer-aided design, and structure-property relationships is envisaged to provide the solution to the current day challenges in the biomedical arena. The traditional methods of fabrication of biomedical devices including casting and mechanical forming have limitations because of the mismatch in micro/microstructure, mechanical, and physical properties with the host site. Additive manufacturing of cellular structured alloys via electron beam melting and laser powder bed fusion has benefits of fabricating patient-specific design that is obtained from the computed tomography scan of the defect site. The discussion in the overview consists of two aspects - the first one describes the underlying reason that motivated 3D printing of implants from the perspective of minimising stress shielding together with the mechanical property requirements, where the mechanical properties of cellular structured implants depend on the cellular architecture and percentage cellular porosity. The second aspect focuses on the biological response of cellular structured devices.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"51 1","pages":"630-640"},"PeriodicalIF":5.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71477524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on tumour cell-derived hybrid exosomes as dasatinib nanocarriers for pancreatic cancer therapy. 肿瘤细胞衍生的混合外泌体作为达沙替尼纳米载体用于癌症治疗的研究。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2023-12-01 Epub Date: 2023-10-05 DOI: 10.1080/21691401.2023.2264358

Xiaofei Zhou, Yuetang Zhuang, Xiaohong Liu, Yaowen Gu, Junting Wang, Yuchen Shi, Li Zhang, Rui Li, Yelin Zhao, Hebing Chen, Jiao Li, Hongjuan Yao, Liang Li

{"title":"Study on tumour cell-derived hybrid exosomes as dasatinib nanocarriers for pancreatic cancer therapy.","authors":"Xiaofei Zhou, Yuetang Zhuang, Xiaohong Liu, Yaowen Gu, Junting Wang, Yuchen Shi, Li Zhang, Rui Li, Yelin Zhao, Hebing Chen, Jiao Li, Hongjuan Yao, Liang Li","doi":"10.1080/21691401.2023.2264358","DOIUrl":"10.1080/21691401.2023.2264358","url":null,"abstract":"Pancreatic ductal adenocarcinoma (PDAC) is one of the leading causes of cancer-related death. Therefore, we intend to explore novel strategies against PDAC. The exosomes-based biomimetic nanoparticle is an appealing candidate served as a drug carrier in cancer treatment, due to its inherit abilities. In the present study, we designed dasatinib-loaded hybrid exosomes by fusing human pancreatic cancer cells derived exosomes with dasatinib-loaded liposomes, followed by characterization for particle size (119.9 ± 6.10 nm) and zeta potential (-11.45 ± 2.24 mV). Major protein analysis from western blot techniques reveal the presence of exosome marker proteins CD9 and CD81. PEGylated hybrid exosomes showed pH-sensitive drug release in acidic condition, benefiting drug delivery to acidic cancer environment. Dasatinib-loaded hybrid exosomes exhibited significantly higher uptake rates and cytotoxicity to parent PDAC cells by two-sample t-test or by one-way ANOVA analysis of variance, as compared to free drug or liposomal formulations. The results from our computational analysis demonstrated that the drug-likeness, ADMET, and protein-ligand binding affinity of dasatinib are verified successfully. Cancer derived hybrid exosomes may serve as a potential therapeutic candidate for pancreatic cancer treatment.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"51 1","pages":"532-546"},"PeriodicalIF":5.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72208207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of shark VNARs targeting the Helicobacter pylori adhesin HpaA. 针对幽门螺杆菌粘连蛋白HpaA的鲨鱼VNARs的鉴定与表征。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2023-09-04 DOI: 10.1080/21691401.2023.2255635

Yanchun Gao, Ruihong Wang, Lin Liu, Shitao Feng, Xiaozhi Xi, Wengong Yu, Yuchao Gu, Ye Wang

{"title":"Identification and characterization of shark VNARs targeting the Helicobacter pylori adhesin HpaA.","authors":"Yanchun Gao, Ruihong Wang, Lin Liu, Shitao Feng, Xiaozhi Xi, Wengong Yu, Yuchao Gu, Ye Wang","doi":"10.1080/21691401.2023.2255635","DOIUrl":"https://doi.org/10.1080/21691401.2023.2255635","url":null,"abstract":"Helicobacter pylori (H. pylori) is recognized as a pathogen associated with several gastrointestinal diseases. The current treatments exhibit numerous drawbacks, including antibiotic resistance. H. pylori can adhere to and colonize the gastric mucosa through H. pylori adhesin A (HpaA), and antibodies against HpaA may be an effective therapeutic approach. The variable domain of immunoglobulin new antigen receptor (VNAR) is a novel type of single-domain antibody with a small size, good stability, and easy manufacturability. This study isolated VNARs against HpaA from an immune shark VNAR phage display library. The VNARs can bind both recombinant and native HpaA proteins. The VNARs, 2A2 and 3D6, showed high binding affinities to HpaA with different epitopes. Furthermore, homodimeric bivalent VNARs, biNb-2A2 and biNb-3D6, were constructed to enhance the binding affinity. The biNb-2A2 and biNb-3D6 had excellent stability at gastrointestinal pH conditions. Finally, a sandwich ELISA assay was developed to quantify the HpaA protein using BiNb-2A2 as the capture antibody and BiNb-3D6 as the detection antibody. This study provides a potential foundation for novel alternative approaches to treatment or diagnostics applications of H. pylori infection.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"51 1","pages":"509-519"},"PeriodicalIF":5.8,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10211558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0