Artificial Cells, Nanomedicine, and Biotechnology最新文献_第5页

Studying the effects of secondary metabolites isolated from Cycas thouarsii R.Br. leaves on MDA-MB-231 breast cancer cells. 研究从苏铁（Cycas thouarsii R.Br.）叶片中分离出的次生代谢物对 MDA-MB-231 乳腺癌细胞的影响。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2024-12-01 Epub Date: 2024-01-27 DOI: 10.1080/21691401.2024.2306529

Badriyah Alotaibi, Thanaa A El-Masry, Engy Elekhnawy, Fatma A Mokhtar, Hosam M El-Seadawy, Walaa A Negm

{"title":"Studying the effects of secondary metabolites isolated from Cycas thouarsii R.Br. leaves on MDA-MB-231 breast cancer cells.","authors":"Badriyah Alotaibi, Thanaa A El-Masry, Engy Elekhnawy, Fatma A Mokhtar, Hosam M El-Seadawy, Walaa A Negm","doi":"10.1080/21691401.2024.2306529","DOIUrl":"10.1080/21691401.2024.2306529","url":null,"abstract":"The various therapeutic drugs that are currently utilized for the management of cancer, especially breast cancer, are greatly challenged by the augmented resistance that is either acquired or de novo by the cancer cells owing to the long treatment periods. So, this study aimed at elucidating the possible anticancer potential of four compounds 7, 4', 7'', 4'''-tetra-O-methyl amentoflavone, hesperidin, ferulic acid, and chlorogenic acid that are isolated from Cycas thouarsii leaves n-butanol fraction for the first time. The MTT assay evaluated the cytotoxic action of four isolated compounds against MDA-MB-231 breast cancer cells and oral epithelial cells. Interestingly, ferulic acid revealed the lowest IC50 of 12.52 µg/mL against MDA-MB-231 cells and a high IC50 of 80.2 µg/mL against oral epithelial cells. Also, using an inverted microscope, the influence of ferulic acid was studied on the MDA-MB-231, which revealed the appearance of apoptosis characteristics like shrinkage of the cells and blebbing of the cell membrane. In addition, the flow cytometric analysis showed that the MDA-MB-231 cells stained with Annexin V/PI had a rise in the count of the cells in the early and late apoptosis stages. Moreover, gel electrophoresis detected DNA fragmentation in the ferulic acid-treated cells. Finally, the effect of the compound was tested at the molecular level by qRT-PCR. An upregulation of the pro-apoptotic genes (BAX and P53) and a downregulation of the anti-apoptotic gene (BCL-2) were observed. Consequently, our study demonstrated that these isolated compounds, especially ferulic acid, may be vital anticancer agents, particularly for breast cancer, through its induction of apoptosis through the P53-dependent pathway.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"52 1","pages":"103-113"},"PeriodicalIF":5.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139569794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A bioconvergence study on platinum-free concurrent chemoradiotherapy for the treatment of HPV-negative head and neck carcinoma. 治疗人乳头状瘤病毒阴性头颈癌的无铂同步化放疗生物融合研究。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2024-12-01 Epub Date: 2024-02-05 DOI: 10.1080/21691401.2024.2309233

Alessandra Gonnelli, Patrizia Sarogni, Noemi Giannini, Stefania Linsalata, Fabio Di Martino, Agata Zamborlin, Valentina Frusca, Maria Laura Ermini, Paola Puccini, Valerio Voliani, Fabiola Paiar

{"title":"A bioconvergence study on platinum-free concurrent chemoradiotherapy for the treatment of HPV-negative head and neck carcinoma.","authors":"Alessandra Gonnelli, Patrizia Sarogni, Noemi Giannini, Stefania Linsalata, Fabio Di Martino, Agata Zamborlin, Valentina Frusca, Maria Laura Ermini, Paola Puccini, Valerio Voliani, Fabiola Paiar","doi":"10.1080/21691401.2024.2309233","DOIUrl":"10.1080/21691401.2024.2309233","url":null,"abstract":"Locally advanced head and neck squamous cell carcinoma (LA-HNSCC) is characterized by high rate of recurrence, resulting in a poor survival. Standard treatments are associated with significant toxicities that impact the patient's quality of life, highlighting the urgent need for novel therapies to improve patient outcomes. On this regard, noble metal nanoparticles (NPs) are emerging as promising agents as both drug carriers and radiosensitizers. On the other hand, co-treatments based on NPs are still at the preclinical stage because of the associated metal-persistence.In this bioconvergence study, we introduce a novel strategy to exploit tumour chorioallantoic membrane models (CAMs) in radio-investigations within clinical equipment and evaluate the performance of non-persistent nanoarchitectures (NAs) in combination with radiotherapy with respect to the standard concurrent chemoradiotherapy for the treatment of HPV-negative HNSCCs. A comparable effect has been observed between the tested approaches, suggesting NAs as a potential platinum-free agent in concurrent chemoradiotherapy for HNSCCs. On a broader basis, our bioconvergence approach provides an advance for the translation of Pt-free radiosensitizer to the clinical practice, positively shifting the therapeutic vs. side effects equilibrium for the management of HNSCCs.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"52 1","pages":"122-129"},"PeriodicalIF":5.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139691129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxygenation through oral Ox66 in a two-hit rodent model of respiratory distress. 在呼吸窘迫的两击啮齿动物模型中通过口服 Ox66 获得氧气。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2024-12-01 Epub Date: 2024-02-29 DOI: 10.1080/21691401.2024.2307462

Bjorn K Song, Danuel A Carr, Erica D Bruce, William H Nugent

{"title":"Oxygenation through oral Ox66 in a two-hit rodent model of respiratory distress.","authors":"Bjorn K Song, Danuel A Carr, Erica D Bruce, William H Nugent","doi":"10.1080/21691401.2024.2307462","DOIUrl":"10.1080/21691401.2024.2307462","url":null,"abstract":"Acute respiratory distress syndrome (ARDS) is a complication of pulmonary disease that produces life-threatening hypoxaemia. Despite ventilation and hyperoxic therapies, undetected hypoxia can manifest in capillary beds leading to multi-organ failure. Ox66™ is an ingestible, solid-state form of oxygen designed to supplement oxygen deficits. Twenty-four anaesthetized rats underwent a two-hit model of respiratory distress (ARDS), where a single dose (5 mg/kg) of lipopolysaccharide (LPS) was given intratracheally, and then the respiratory tidal volume was reduced by 40%. After 60 min, animals were randomized to receive Ox66™, or normal saline (NS; vehicle control) via gavage or supplemental inspired oxygen (40% FiO2). A second gavage was administered at 120 min. Cardiovascular function and blood oximetry/chemistry were measured alongside the peripheral spinotrapezius muscle's interstitial oxygenation (PISFO2). ARDS reduced mean arterial pressure (MAP) and PISFO2 compared to baseline (BL) for all treatment groups. Treatment with Ox66 or NS did not improve MAP, but 40% FiO2 caused a rapid return to BL. PISFO2 improved after treatment with Ox66™ and 40% FiO2 and remained elevated for both groups against NS until study conclusion. Both oxygen treatments also suppressed the inflammatory response to LPS, suggesting that Ox66™ can deliver therapeutically-impactful levels of oxygen in situations of pulmonary dysfunction.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"52 1","pages":"114-121"},"PeriodicalIF":5.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139995452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the anticancer and antioxidant potential of gold nanoparticles synthesized from Pterocarpus marsupium bark extract against oral squamous cell carcinoma. 探讨紫檀树皮提取物合成的金纳米粒子对口腔鳞状细胞癌的抗癌和抗氧化潜力

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2024-12-01 Epub Date: 2024-10-25 DOI: 10.1080/21691401.2024.2416951

Smrutipragnya Samal, Rajesh Kumar Meher, Pratyush Kumar Das, Santosh Kumar Swain, Debasmita Dubey, Mohd Shahnawaz Khan, Bigyan Ranjan Jali

{"title":"Exploring the anticancer and antioxidant potential of gold nanoparticles synthesized from Pterocarpus marsupium bark extract against oral squamous cell carcinoma.","authors":"Smrutipragnya Samal, Rajesh Kumar Meher, Pratyush Kumar Das, Santosh Kumar Swain, Debasmita Dubey, Mohd Shahnawaz Khan, Bigyan Ranjan Jali","doi":"10.1080/21691401.2024.2416951","DOIUrl":"https://doi.org/10.1080/21691401.2024.2416951","url":null,"abstract":"Oral squamous cell carcinoma (OSCC) is a disease of significant concern with higher mortality rates. Conventional treatment approaches have several drawbacks, leading to the opening of new research avenues in the field of nanoparticle-based cancer therapeutics. The study aimed at the synthesis of gold nanoparticles (Pm-AuNPs) from the aqueous bark extract of Pterocarpus marsupium, followed by its characterization and in vitro anticancer evaluation against OSCC. The synthesized Pm-AuNPs were characterized using UV-visible spectroscopy, particle size analyser, zeta potential, FTIR and SEM techniques. The anticancer potential of the Pm-AuNPs was evaluated against OSCC cell lines (SCC29b, SSC154 and OECM-1) through in vitro assays. The IC50 value was found to be 25 ± 1.2, 45 ± 1.5 and 75 ± 2.1 µg/mL for the three OSCC cell lines, elucidating Pm-AuNPs cytotoxic effects and mechanism of action. Intracellular ROS and SOX detection, mitochondrial transmembrane potential analysis and apoptosis detection were used to confirm the activity of Pm-AuNPs against OSCC. Acute toxicity studies on Wistar rats confirmed the non-toxic nature of the Pm-AuNPs at a higher dose concentration up to 2000 mg/kg body weight. The findings underscore Pm-AuNPs as promising candidates for future anticancer therapeutics, providing insights into their mechanism of action and therapeutic efficacy against OSCC.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"52 1","pages":"512-528"},"PeriodicalIF":4.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three musketeers of PDA-based MRI contrasting and therapy. 基于 PDA 的磁共振成像对比和治疗三剑客。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2024-12-01 Epub Date: 2024-05-25 DOI: 10.1080/21691401.2024.2356773

Magdalena J Bigaj-Józefowska, Tomasz Zalewski, Karol Załęski, Emerson Coy, Marcin Frankowski, Radosław Mrówczyński, Bartosz F Grześkowiak

{"title":"Three musketeers of PDA-based MRI contrasting and therapy.","authors":"Magdalena J Bigaj-Józefowska, Tomasz Zalewski, Karol Załęski, Emerson Coy, Marcin Frankowski, Radosław Mrówczyński, Bartosz F Grześkowiak","doi":"10.1080/21691401.2024.2356773","DOIUrl":"https://doi.org/10.1080/21691401.2024.2356773","url":null,"abstract":"Polydopamine (PDA) stands as a versatile material explored in cancer nanomedicine for its unique properties, offering opportunities for multifunctional drug delivery platforms. This study explores the potential of utilizing a one-pot synthesis to concurrently integrate Fe, Gd and Mn ions into porous PDA-based theranostic drug delivery platforms called Ferritis, Gadolinis and Manganis, respectively. Our investigation spans the morphology, magnetic properties, photothermal characteristics and cytotoxicity profiles of those potent nanoformulations. The obtained structures showcase a spherical morphology, robust magnetic response and promising photothermal behaviour. All of the presented nanoparticles (NPs) display pronounced paramagnetism, revealing contrasting potential for MRI imaging. Relaxivity values, a key determinant of contrast efficacy, demonstrated competitive or superior performance compared to established, used contrasting agents. These nanoformulations also exhibited robust photothermal properties under near infra-red irradiation, showcasing their possible application for photothermal therapy of cancer. Our findings provide insights into the potential of metal-doped PDA NPs for cancer theranostics.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"52 1","pages":"321-333"},"PeriodicalIF":5.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141096867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green synthesized silver nanoparticles of Terminalia bellirica leaves extract: synthesis, characterization, in-silico studies, and antimalarial activity. 槟榔叶提取物的绿色合成银纳米粒子：合成、表征、硅内研究和抗疟活性。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2024-12-01 Epub Date: 2024-05-02 DOI: 10.1080/21691401.2024.2339429

Sujeet Singh, Hemant Arya, Welka Sahu, K Sony Reddy, Surendra Nimesh, Bader Saud Alotaibi, Mohammed Ageeli Hakami, Hassan H Almasoudi, Khater Balatone Gezira Hessien, Mohammad Raghibul Hasan, Summya Rashid, Tarun Kumar Bhatt

{"title":"Green synthesized silver nanoparticles of Terminalia bellirica leaves extract: synthesis, characterization, in-silico studies, and antimalarial activity.","authors":"Sujeet Singh, Hemant Arya, Welka Sahu, K Sony Reddy, Surendra Nimesh, Bader Saud Alotaibi, Mohammed Ageeli Hakami, Hassan H Almasoudi, Khater Balatone Gezira Hessien, Mohammad Raghibul Hasan, Summya Rashid, Tarun Kumar Bhatt","doi":"10.1080/21691401.2024.2339429","DOIUrl":"https://doi.org/10.1080/21691401.2024.2339429","url":null,"abstract":"Malaria is a mosquito-borne infectious disease that is caused by the Plasmodium parasite. Most of the available medication are losing their efficacy. Therefore, it is crucial to create fresh leads to combat malaria. Green silver nanoparticles (AgNPs) have recently attracted a lot of attention in biomedical research. As a result, green mediated AgNPs from leaves of Terminalia bellirica, a medicinal plant with purported antimalarial effects, were used in this investigation. Initially, cysteine-rich proteins from Plasmodium species were studied in silico as potential therapeutic targets. With docking scores between -9.93 and -11.25 kcal/mol, four leaf constituents of Terminalia bellirica were identified. The green mediated silver nanoparticles were afterward produced using leaf extract and were further examined using UV-vis spectrophotometer, DLS, Zeta potential, FTIR, XRD, and FESEM. The size of synthesized TBL-AgNPs was validated by the FESEM results; the average size of TBL-AgNPs was around 44.05 nm. The zeta potential study also supported green mediated AgNPs stability. Additionally, Plasmodium falciparum (3D7) cultures were used to assess the antimalarial efficacy, and green mediated AgNPs could effectively inhibit the parasitized red blood cells (pRBCs). In conclusion, this novel class of AgNPs may be used as a potential therapeutic replacement for the treatment of malaria.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"52 1","pages":"238-249"},"PeriodicalIF":5.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140849274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The identification of metabolites from gut microbiota in coronary heart disease via network pharmacology. 通过网络药理学鉴定冠心病中肠道微生物群的代谢物。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2024-12-01 Epub Date: 2024-02-27 DOI: 10.1080/21691401.2024.2319827

Hao-Ming Zhou, Xin-Yu Yang, Shi-Jun Yue, Wen-Xiao Wang, Qiao Zhang, Ding-Qiao Xu, Jia-Jia Li, Yu-Ping Tang

{"title":"The identification of metabolites from gut microbiota in coronary heart disease via network pharmacology.","authors":"Hao-Ming Zhou, Xin-Yu Yang, Shi-Jun Yue, Wen-Xiao Wang, Qiao Zhang, Ding-Qiao Xu, Jia-Jia Li, Yu-Ping Tang","doi":"10.1080/21691401.2024.2319827","DOIUrl":"10.1080/21691401.2024.2319827","url":null,"abstract":"Although the gut microbial metabolites exhibit potential effects on coronary heart disease (CHD), the underlying mechanism remains unclear. In this study, the active gut microbial metabolites acting on CHD and their potential mechanisms of action were explored through a network pharmacological approach. We collected a total of 208 metabolites from the gutMgene database and 726 overlapping targets from the similarity ensemble approach (SEA) and SwissTargetPrediction (STP) database, and ultimately identified 610 targets relevant to CHD. In conjunction with the gutMGene database, we identified 12 key targets. The targets of exogenous substances were removed, and 10 core targets involved in CHD were eventually retained. The microbiota-metabolites-targets-signalling pathways network analysis revealed that C-type lectin receptor signalling pathway, Lachnospiraceae, Escherichia, mitogen-activated protein kinase 1, prostaglandin-endoperoxidase synthase 2, phenylacetylglutamine and alcoholic acid are notable components of CHD and play important roles in the development of CHD. The results of molecular docking experiments demonstrated that AKT1-glycocholic acid and PTGS2-phenylacetylglutamine complexes may act on C-type lectin receptor signalling pathways. In this study, the key substances and potential mechanisms of gut microbial metabolites were analysed via network pharmacological methods, and a scientific basis and comprehensive idea were provided for the effects of gut microbial metabolites on CHD.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"52 1","pages":"145-155"},"PeriodicalIF":5.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139982243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in nanotechnology-assisted photodynamic therapy for neurological disorders: a comprehensive review. 纳米技术辅助光动力疗法治疗神经系统疾病的进展：综述。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2024-12-01 Epub Date: 2024-01-18 DOI: 10.1080/21691401.2024.2304814

Abdul Nasir, Mujeeb Ur Rehman, Tamreez Khan, Mansoor Husn, Manzar Khan, Ahmad Khan, Abdifatah Mohamed Nuh, Wei Jiang, Hafiz Muhammad Umer Farooqi, Qain Bai

{"title":"Advances in nanotechnology-assisted photodynamic therapy for neurological disorders: a comprehensive review.","authors":"Abdul Nasir, Mujeeb Ur Rehman, Tamreez Khan, Mansoor Husn, Manzar Khan, Ahmad Khan, Abdifatah Mohamed Nuh, Wei Jiang, Hafiz Muhammad Umer Farooqi, Qain Bai","doi":"10.1080/21691401.2024.2304814","DOIUrl":"10.1080/21691401.2024.2304814","url":null,"abstract":"Neurological disorders such as neurodegenerative diseases and nervous system tumours affect more than one billion people throughout the globe. The physiological sensitivity of the nervous tissue limits the application of invasive therapies and leads to poor treatment and prognosis. One promising solution that has generated attention is Photodynamic therapy (PDT), which can potentially revolutionise the treatment landscape for neurological disorders. PDT attracted substantial recognition for anticancer efficacy and drug conjugation for targeted drug delivery. This review thoroughly explained the basic principles of PDT, scientific interventions and advances in PDT, and their complicated mechanism in treating brain-related pathologies. Furthermore, the merits and demerits of PDT in the context of neurological disorders offer a well-rounded perspective on its feasibility and challenges. In conclusion, this review encapsulates the significant potential of PDT in transforming the treatment landscape for neurological disorders, emphasising its role as a non-invasive, targeted therapeutic approach with multifaceted applications.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"52 1","pages":"84-103"},"PeriodicalIF":5.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139485073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors affecting response variables with emphasis on drug release and loading for optimization of liposomes. 影响响应变量的因素，重点是优化脂质体的药物释放和负载。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2024-12-01 Epub Date: 2024-06-04 DOI: 10.1080/21691401.2024.2360634

Shantanu Pande

引用次数: 0

High-throughput single-cell screening of viable hybridomas and patient-derived antibody-secreting cells using punchable microwells. 使用可打孔微孔对有活力的杂交瘤和源自患者的抗体分泌细胞进行高通量单细胞筛选。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2024-12-01 Epub Date: 2024-08-29 DOI: 10.1080/21691401.2024.2395815

Kaat Rubben, Ann-Sophie Vander Plaetsen, Ruben Almey, Olivier Tytgat, Koen Deserranno, Jamie Debaere, Delphine Diana Acar, Philip Meuleman, Dieter Deforce, Filip Van Nieuwerburgh

{"title":"High-throughput single-cell screening of viable hybridomas and patient-derived antibody-secreting cells using punchable microwells.","authors":"Kaat Rubben, Ann-Sophie Vander Plaetsen, Ruben Almey, Olivier Tytgat, Koen Deserranno, Jamie Debaere, Delphine Diana Acar, Philip Meuleman, Dieter Deforce, Filip Van Nieuwerburgh","doi":"10.1080/21691401.2024.2395815","DOIUrl":"https://doi.org/10.1080/21691401.2024.2395815","url":null,"abstract":"Monoclonal antibodies (mAbs) hold significant potential as therapeutic agents and are invaluable tools in biomedical research. However, the lack of efficient high-throughput screening methods for single antibody-secreting cells (ASCs) has limited the diversity of available antibodies. Here, we introduce a novel, integrated workflow employing self-seeding microwells and an automated microscope-puncher system for the swift, high-throughput screening and isolation of single ASCs. The system allows for the individual screening and isolation of up to 6,400 cells within approximately one day, with the opportunity for parallelization and efficient upscaling. We successfully applied this workflow to both hybridomas and human patient-derived B cells, enabling subsequent clonal expansion or antibody sequence analysis through an optimized, single-cell nested reverse transcription-polymerase chain reaction (RT-PCR) procedure. By providing a time-efficient and more streamlined single ASC screening and isolation process, our workflow holds promise for driving forward progress in mAb development.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"52 1","pages":"426-436"},"PeriodicalIF":4.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0