Artificial Cells, Nanomedicine, and Biotechnology最新文献_第5页

Nitrogen-doped carbon quantum dots as a novel treatment for black fungal bone infections (Mucormycosis): in vitro and in vivo study. 氮掺杂碳量子点作为黑真菌骨感染（黏菌病）的新型治疗方法：体外和体内研究。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2024-12-01 Epub Date: 2024-02-29 DOI: 10.1080/21691401.2024.2318212

Amany Belal, Atiah H Almalki, Ahmed A Farghali, Rehab Mahmoud, R R Atta, Abeer Enaiet Allah, Walid Hamdy Hassan, Sangmin Lee, Amna A Kotp, Doaa Essam, Ahmed H E Hassan, Mohammed M Ghoneim, Fatma I Abo El-Ela, Abdalla Abdelwahab

{"title":"Nitrogen-doped carbon quantum dots as a novel treatment for black fungal bone infections (Mucormycosis): in vitro and in vivo study.","authors":"Amany Belal, Atiah H Almalki, Ahmed A Farghali, Rehab Mahmoud, R R Atta, Abeer Enaiet Allah, Walid Hamdy Hassan, Sangmin Lee, Amna A Kotp, Doaa Essam, Ahmed H E Hassan, Mohammed M Ghoneim, Fatma I Abo El-Ela, Abdalla Abdelwahab","doi":"10.1080/21691401.2024.2318212","DOIUrl":"10.1080/21691401.2024.2318212","url":null,"abstract":"Most fungal bone and joint infections (arthritis) are caused by Mucormycosis (Mucor indicus). These infections may be difficult to treat and may lead to chronic bone disorders and disabilities, thus the use of new antifungal materials in bone disorders is vital, particularly in immunocompromised individuals, such as those who have contracted coronavirus disease 2019 (COVID-19). Herein, we reported for the first time the preparation of nitrogen-doped carbon quantum dots (N/CQDs) and a nitrogen-doped mesoporous carbon (N/MC) using a quick micro-wave preparation and hydrothermal approach. The structure and morphology were analysed using X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and surface area analyser. Minimum inhibitory concentration (MIC), disc diffusion tests, minimum fungicidal concentration (MFC) and antifungal inhibitory percentages were measured to investigate the antifungal activity of N/CQDs and N/MC nanostructures. In addition to the in vivo antifungal activity in rats as determined by wound induction and infection, pathogen count and histological studies were also performed. According to in vitro and in vivo testing, both N/CQDs with small size and N/MC with porous structure had a significant antifungal impact on a variety of bone-infecting bacteria, including Mucor infection. In conclusion, the present investigation demonstrates that functional N/CQDs and N/MC are effective antifungal agents against a range of microbial pathogenic bone disorders in immunocompromised individuals, with stronger and superior fungicidal activity for N/CQDs than N/MC in vitro and in vivo studies.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"52 1","pages":"131-144"},"PeriodicalIF":5.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139995451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological evaluation of ZrO₂ composites modified with different ceramics additives. 用不同陶瓷添加剂改性的 ZrO2 复合材料的生物学评价。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2024-12-01 Epub Date: 2024-11-04 DOI: 10.1080/21691401.2024.2422870

Magdalena Ziąbka, Agnieszka Wojteczko, Barbara Zagrajczuk, Aleksandra Benko, Sebastian Komarek, Elżbieta Menaszek

引用次数: 0

Mikania micrantha silver nanoparticles exhibit anticancer activities against human lung adenocarcinoma via caspase-mediated apoptotic cell death. 薇甘菊银纳米颗粒通过卡巴酶介导的细胞凋亡对人类肺腺癌具有抗癌活性。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2024-12-01 Epub Date: 2024-03-11 DOI: 10.1080/21691401.2024.2325942

Fanai Lalsangpuii, Samuel Lalthazuala Rokhum, Fanai Nghakliana, Joseph V L Ruatpuia, Lalchhandami Tochhawng, Amit Kumar Trivedi, Ralte Lalfakzuala, Zothan Siama

{"title":"Mikania micrantha silver nanoparticles exhibit anticancer activities against human lung adenocarcinoma via caspase-mediated apoptotic cell death.","authors":"Fanai Lalsangpuii, Samuel Lalthazuala Rokhum, Fanai Nghakliana, Joseph V L Ruatpuia, Lalchhandami Tochhawng, Amit Kumar Trivedi, Ralte Lalfakzuala, Zothan Siama","doi":"10.1080/21691401.2024.2325942","DOIUrl":"10.1080/21691401.2024.2325942","url":null,"abstract":"Green-mediated synthesis of nanoparticles has earned a promising role in the area of nanotechnology due to their biomedical applications. This study describes the synthesis of silver nanoparticles (AgNPs) using Mikania micrantha leaf extract and its functional activities against cancer. The synthesis of AgNPs was confirmed using Ultraviolet-Visible (UV-Vis) spectrum that exhibited an absorption band at 459 nm. The bioactive compounds of M. micrantha leaf extract that functioned as reducing and capping agents were confirmed by a shift in the absorption bands in Fourier Transform Infra-red Spectroscopy (FT-IR). Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) studies validated the spherical shape and size of AgNPs, respectively. Energy Dispersive Spectroscopy (EDS) analysis revealed the presence of elemental silver. The crystalline nature of AgNPs was confirmed by the X-ray Diffraction Analysis (XRD). AgNPs effectively induced cytotoxicity and prevented A549 cell colony formation in a dose-dependent manner. Treatment of A549 cells with AgNPs also increased DNA damage, which was coupled with elevated lipid peroxidation and decreased antioxidant enzymes such as glutathione (GSH), glutathione-s-transferase (GST), and superoxide dismutase (SOD). Following AgNPs treatment, the mRNA expression levels of the pro-apoptotic genes as well as the activities of caspases were significantly elevated in A549 cells while the expression levels of anti-apoptotic genes were downregulated. Our study demonstrates the potential of the synthesised AgNPs for cancer therapy possibly targeting the apoptotic pathway.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"52 1","pages":"186-200"},"PeriodicalIF":5.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140093311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in polysaccharide-based drug delivery systems for cancer therapy: a comprehensive review.

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2024-12-01 Epub Date: 2024-12-05 DOI: 10.1080/21691401.2024.2436350

Chou-Yi Hsu, Omer Qutaiba B Allela, Ali M Hussein, Mohammed Ahmed Mustafa, Mandeep Kaur, Mohd Alaraj, Ali Fawzi Al-Hussainy, Usama Kadem Radi, Mohammed Ubaid, Ameer Hassan Idan, Fahad Alsaikhan, Asghar Narmani, Bagher Farhood

{"title":"Recent advances in polysaccharide-based drug delivery systems for cancer therapy: a comprehensive review.","authors":"Chou-Yi Hsu, Omer Qutaiba B Allela, Ali M Hussein, Mohammed Ahmed Mustafa, Mandeep Kaur, Mohd Alaraj, Ali Fawzi Al-Hussainy, Usama Kadem Radi, Mohammed Ubaid, Ameer Hassan Idan, Fahad Alsaikhan, Asghar Narmani, Bagher Farhood","doi":"10.1080/21691401.2024.2436350","DOIUrl":"10.1080/21691401.2024.2436350","url":null,"abstract":"Cancer has a high rate of incidence and mortality throughout the world. Although several conventional approaches have been developed for the treatment of cancer, such as surgery, chemotherapy, radiotherapy and thermal therapy, they have remarkable disadvantages which result in inefficient treatment of cancer. For example, immunogenicity, prolonged treatment, non-specificity, metastasis and high cost of treatment, are considered as the major drawbacks of chemotherapy. Therefore, there is a fundamental requirement for the development of breakthrough technologies for cancer suppression. Polysaccharide-based drug delivery systems (DDSs) are the most reliable drug carriers for cancer therapy. Polysaccharides, as a kind of practical biomaterials, are divided into several types, including chitosan, alginates, dextran, hyaluronic acid, cyclodextrin, pectin, etc. Polysaccharides are extracted from different natural resources (like herbal, marine, microorganisms, etc.). The potential features of polysaccharides have made them reliable candidates for therapeutics delivery to cancer sites; the simple purification, ease of modification and functionalization, hydrophilicity, serum stability, appropriate drug loading capacity, biocompatibility, bioavailability, biodegradability and stimuli-responsive and sustained drug release manner are considerable aspects of these biopolymers. This review highlights the practical applications of polysaccharides-based DDSs in pharmaceutical science and cancer therapy.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"52 1","pages":"564-586"},"PeriodicalIF":4.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142784107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of ferroptosis-related gene signatures for identifying potential biomarkers and immune cell infiltration in osteoarthritis. 构建铁蛋白沉积相关基因特征，以确定骨关节炎的潜在生物标志物和免疫细胞浸润。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2024-09-11 DOI: 10.1080/21691401.2024.2402298

Yali Yu,Guixiang Dong,Yanli Niu

{"title":"Construction of ferroptosis-related gene signatures for identifying potential biomarkers and immune cell infiltration in osteoarthritis.","authors":"Yali Yu,Guixiang Dong,Yanli Niu","doi":"10.1080/21691401.2024.2402298","DOIUrl":"https://doi.org/10.1080/21691401.2024.2402298","url":null,"abstract":"Osteoarthritis (OA) is a comprehensive joint disorder. The specific genes that trigger OA and the strategies for its effective management are not fully understood. This study focuses on identifying key genes linked to iron metabolism that could influence both the diagnosis and therapeutic approaches for OA. Analysis of GEO microarray data and iron metabolism genes identified 15 ferroptosis-related DEGs, enriched in hypoxia and HIF-1 pathways. Ten key hub genes (ATM, GCLC, PSEN1, CYBB, ATG7, MAP1LC3B, PLIN2, GRN, APOC1, SIAH2) were identified. Through stepwise regression, we screened 4 out of the above 10 genes, namely, GCLC, GRN, APOC1, and SIAH2, to obtain the optimal model. AUROCs for diagnosis of OA for the four hub genes were 0.81 and 0.80 of training and validation sets, separately. According to immune infiltration results, OA was related to significantly increased memory B cells, M0 macrophages, regulatory T cells, and resting mast cells but decreased activated dendritic cells. The four hub genes showed a close relation to them. It is anticipated that this model will aid in diagnosing osteoarthritis by assessing the expression of specific genes in blood samples. Moreover, studying these hub genes may further elucidate the pathogenesis of osteoarthritis.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"12 1","pages":"449-461"},"PeriodicalIF":5.8,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142176649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring fibroblast interactions on nanocrystalline surfaces in physiological environments through a phenomenological lens 通过现象学视角探索生理环境中纳米晶体表面上成纤维细胞的相互作用

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2024-04-08 DOI: 10.1080/21691401.2024.2338127

R.D.K Misra, Aladin M. Boriek

引用次数: 0

Synthesis and characterization of Fe3O4@SiO2@PDA@Ag core–shell nanoparticles and biological application on human lung cancer cell line and antibacterial strains Fe3O4@SiO2@PDA@Ag 核壳纳米粒子的合成与表征及其在人类肺癌细胞系和抗菌菌株上的生物应用

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2023-12-29 DOI: 10.1080/21691401.2023.2295534

Snigdha Singh, Tanya Goel, Aarushi Singh, Heerak Chugh, Nayanika Chakraborty, Indrajit Roy, Manisha Tiwari, Ramesh Chandra

引用次数: 0

Niosomes gel of apigenin to improve the topical delivery: development, optimization, ex vivo permeation, antioxidant study, and in vivo evaluation. 芹菜素Niosomes凝胶改善局部给药：开发、优化、离体渗透、抗氧化研究和体内评价。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2023-12-01 Epub Date: 2023-11-01 DOI: 10.1080/21691401.2023.2274526

Omar Awad Alsaidan, Ameeduzzafar Zafar, Rayan Hamood Al-Ruwaili, Mohd Yasir, Sami I Alzarea, Aseel Awad Alsaidan, Lubhan Singh, Mohammad Khalid

{"title":"Niosomes gel of apigenin to improve the topical delivery: development, optimization, ex vivo permeation, antioxidant study, and in vivo evaluation.","authors":"Omar Awad Alsaidan, Ameeduzzafar Zafar, Rayan Hamood Al-Ruwaili, Mohd Yasir, Sami I Alzarea, Aseel Awad Alsaidan, Lubhan Singh, Mohammad Khalid","doi":"10.1080/21691401.2023.2274526","DOIUrl":"https://doi.org/10.1080/21691401.2023.2274526","url":null,"abstract":"Niosomes (NS) are the promising and novel carrier of the drug for effective transdermal delivery. Apigenin (AN) is a natural bioactive compound and has various pharmacological activities. AN is poorly water soluble which directly affects therapeutic efficacy. The aim of this research work was to develop the AN-NS gel to improve transdermal delivery. The thin-film hydration method was used for the development of AN-NS. The optimized AN-NS (AN-NS2) has a vesicle size of 272.56 ± 12.49 nm, PDI is 0.249, zeta potential is -38.7 mV, and entrapment efficiency of 86.19 ± 1.51%. The FTIR spectra of the AN-NS2 depicted that AN encapsulated in the NS matrix. AN-NS2 formulation was successfully incorporated into chitosan gel and evaluated. The optimized AN-NS2 gel (AN-NS2G4) has 2110 ± 14cps of viscosity, 10.40 ± 0.21g.cm/sec of spreadability, and 99.65 ± 0.53% of drug content. AN-NS2G4 displayed significantly (p < 0.05) higher AN released (67.64 ± 3.03%) than pure AN-gel (37.31 ± 2.87%). AN-NS2G4 showed the Korsmeyer Peppas release model. AN-NS2G4 displayed significantly (p < 0.05) higher antioxidant activity (90.72%) than pure AN (64.53%) at 300 µg/ml. AN-NS2G4 displayed significantly (p < 0.05) higher % inhibition of swelling than pane AN-gel in carrageenin-induced paw oedema in rats. The finding concluded that niosomes-laden gel is a good carrier of drugs to improve transdermal delivery and therapeutic efficacy.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"51 1","pages":"604-617"},"PeriodicalIF":5.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71420272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An impedance flow cytometry with integrated dual microneedle for electrical properties characterization of single cell. 一种集成双微针的阻抗流式细胞仪，用于单细胞的电学特性表征。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2023-12-01 DOI: 10.1080/21691401.2023.2239274

Muhammad Asraf Mansor, Mohd Ridzuan Ahmad, Michal Petrů, Seyed Saeid Rahimian Koloor

{"title":"An impedance flow cytometry with integrated dual microneedle for electrical properties characterization of single cell.","authors":"Muhammad Asraf Mansor, Mohd Ridzuan Ahmad, Michal Petrů, Seyed Saeid Rahimian Koloor","doi":"10.1080/21691401.2023.2239274","DOIUrl":"10.1080/21691401.2023.2239274","url":null,"abstract":"Electrical characteristics of living cells have been proven to reveal important details about their internal structure, charge distribution and composition changes in the cell membrane, as well as the extracellular context. An impedance flow cytometry is a common approach to determine the electrical properties of a cell, having the advantage of label-free and high throughput. However, the current techniques are complex and costly for the fabrication process. For that reason, we introduce an integrated dual microneedle-microchannel for single-cell detection and electrical properties extraction. The dual microneedles utilized a commercially available tungsten needle coated with parylene. When a single cell flows through the parallel-facing electrode configuration of the dual microneedle, the electrical impedance at multiple frequencies is measured. The impedance measurement demonstrated the differential of normal red blood cells (RBCs) with three different sizes of microbeads at low and high frequencies, 100 kHz and 2 MHz, respectively. An electrical equivalent circuit model (ECM) was used to determine the unique membrane capacitance of individual cells. The proposed technique demonstrated that the specific membrane capacitance of an RBC is 9.42 mF/m-2, with the regression coefficients, <math><mi>ρ</mi></math> at 0.9895. As a result, this device may potentially be used in developing countries for low-cost single-cell screening and detection.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"51 1","pages":"371-383"},"PeriodicalIF":5.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9944816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green synthesis, characterisation and antibacterial activities of Strobilanthes crispus-mediated silver nanoparticles (SC-AGNPS) against selected bacteria. 脆叶牛膝介导的银纳米粒子（SC-AGNPS）的绿色合成、表征及其对选定细菌的抗菌活性。

IF 5.8 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2023-12-01 Epub Date: 2023-10-17 DOI: 10.1080/21691401.2023.2268167

Rohazila Mohamad Hanafiah, Siti Aisyah Abd Ghafar, Vuanghao Lim, Siti Nor Asma Musa, Fahmi Yakop, Arif Haikal Hairil Anuar

{"title":"Green synthesis, characterisation and antibacterial activities of Strobilanthes crispus-mediated silver nanoparticles (SC-AGNPS) against selected bacteria.","authors":"Rohazila Mohamad Hanafiah, Siti Aisyah Abd Ghafar, Vuanghao Lim, Siti Nor Asma Musa, Fahmi Yakop, Arif Haikal Hairil Anuar","doi":"10.1080/21691401.2023.2268167","DOIUrl":"10.1080/21691401.2023.2268167","url":null,"abstract":"This study aims to characterize and determine the antibacterial activities of synthesized Strobilanthes crispus-mediated AgNPs (SC-AgNPs) against Streptococcus mutans, Escherichia coli and Pseudomonas aeruginosa. S. crispus water extract acts as a reducing and capping agent in the synthesis of AgNPs. The synthesized AgNPs were characterized by using UV-Vis spectrophotometer, dynamic light scattering (DLS), field emission scanning electron microscope (FESEM), X-ray diffractometer (XRD) and Fourier transform infra-red (FTIR). FESEM images showed a rough surface with a spherical shape. The average size distribution of 75.25 nm with a polydispersity index (PDI) of 0.373. XRD analysis matched the face-centred cubic structure of silver. FTIR analysis revealed a shifted peak from 1404.99 to 1345.00 cm-1. MIC and MBC values of SC-AgNPs were 1.25 mg/mL and 2.5 mg/mL against E. coli, P. aeruginosa and S. mutans, respectively. Time-kill assay showed that SC-AgNPs significantly reduced bacterial growth as compared to non-treated bacteria. Morphologies of bacteria treated with SC-AgNPs were shrunk, lysed, irregular and smaller as compared to control. SC-AgNPs significantly disrupted the gene expression of eae A, gtf B and Pel A (p < 0.05). This study indicated that the synthesized SC-AgNPs were stable with enhanced antibacterial activities.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"51 1","pages":"549-559"},"PeriodicalIF":5.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0