Bangladesh Journal of Pharmacology最新文献

2-Methoxy-6-acetyl-7-methyljuglone, a natural naphthoquinone from Polygonum cuspidatum, protects against 6-OHDA-induced neurotoxicity in PC12 cells 2-甲氧基-6-乙酰基-7-甲基核桃酮是虎爪蓼中的一种天然萘醌，可保护PC12细胞免受6- ohda诱导的神经毒性

4区医学

Bangladesh Journal of Pharmacology Pub Date : 2026-03-02 DOI: 10.3329/bjp.v21i1.86818

Yuanfeng Fu, Weifeng Xu, Dongmeng Liu, Jiaolin Bao, Ren-Bo Ding

{"title":"2-Methoxy-6-acetyl-7-methyljuglone, a natural naphthoquinone from Polygonum cuspidatum, protects against 6-OHDA-induced neurotoxicity in PC12 cells","authors":"Yuanfeng Fu, Weifeng Xu, Dongmeng Liu, Jiaolin Bao, Ren-Bo Ding","doi":"10.3329/bjp.v21i1.86818","DOIUrl":"https://doi.org/10.3329/bjp.v21i1.86818","url":null,"abstract":"This study investigated the neuroprotective effects of 2-methoxy-6-acetyl-7-methyljuglone (MAM, a compound isolated from Polygonum cuspidatum) in 6-hydroxydopamine (6-OHDA)-injuried pheochromocytoma (PC12) cells. 6-OHDA treatment significantly induced cell death, apoptosis and mitochondrial membrane potential loss. MAM pretreatment significantly rescued PC12 cell viability, inhibited the 6-OHDA-induced apoptosis and depolarization in mitochondrial membrane potential. Results also demonstrated that 6-OHDA significantly induced ERK and NF-κB phosphorylation, while MAM pretreatment markedly reduced the 6-OHDA-elevated ERK and NF-κB phosphorylation levels. This suggested that MAM exerted neuroprotective effects likely by suppressing ERK-related oxidative stress and NF-κB-mediated neuroinflammation, thereby protecting neuronal cells from 6-OHDA-induced apoptosis. Collectively, these results suggested that MAM exerted a neuroprotective effect in 6-OHDA-induced Parkinson’s disease cell model by suppressing ERK and NF-κB-mediated apoptotic mechanisms.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":"21 1","pages":"20-27"},"PeriodicalIF":0.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://banglajol.info/index.php/BJP/article/download/86818/57315","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147381882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

1α,25-Dihydroxyvitamin D3 reduces uropathogenic E. coli persistence in bladder epithelial cells by restoring lysosome acidification 1α，25-二羟基维生素D3通过恢复溶酶体酸化减少尿路致病性大肠杆菌在膀胱上皮细胞中的持久性

4区医学

Bangladesh Journal of Pharmacology Pub Date : 2025-04-25 DOI: 10.3329/bjp.v20i1.79655

Hao Yin, Xue Yi, Xin Wang

引用次数: 0

ML098 enhances intracellular bacterial clearance in bladder epithelial cells by activating Rab7 and promoting lysosomal biogenesis ML098通过激活Rab7和促进溶酶体生物发生，增强膀胱上皮细胞内细菌清除

4区医学

Bangladesh Journal of Pharmacology Pub Date : 2025-02-25 DOI: 10.3329/bjp.v19i4.77475

Hao Yin, Jiaoli Zhu, Jiamin Yin, Xue Yi, Xin Wang

{"title":"ML098 enhances intracellular bacterial clearance in bladder epithelial cells by activating Rab7 and promoting lysosomal biogenesis","authors":"Hao Yin, Jiaoli Zhu, Jiamin Yin, Xue Yi, Xin Wang","doi":"10.3329/bjp.v19i4.77475","DOIUrl":"https://doi.org/10.3329/bjp.v19i4.77475","url":null,"abstract":"The aim of this study was to investigate the effect of ML098 on the intra-cellular bacterial count of bladder epithelial cells infected with uropathogenic Escherichia coli. The number of intracellular bacteria were counted after cell lysis present in coated Luria-Bertani agar plates. Lysotracker red was used to detect the numbers of lysosomes in cells. The expression of lysosome associated membrane protein 1 within cells was detected by western blot and q-PCR methods. Rab7 pulldown activation assay was used to detect the expression of intracellular active Rab7. The results showed that ML098 (1 µM) could reduce the number of intracellular bacteria in bladder epithelial cells 6 hours after E. coli infection (p<0.05), increased the number of lysosomes in cells (p<0.05), and increased the expression of intracellular active Rab7 (p<0.05). Therefore, ML098 can promote intracellular bacterial clearance, possibly by increasing the expression of active Rab7 in cells, thereby increasing the number of lysosomes in bladder epithelial cells.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":"19 4","pages":"107-113"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://banglajol.info/index.php/BJP/article/download/77475/52383","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147904058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prednisone inhibits osteosarcoma cell by regulating the Wnt/β-catenin signaling pathway 强的松通过调节Wnt/β-catenin信号通路抑制骨肉瘤细胞

4区医学

Bangladesh Journal of Pharmacology Pub Date : 2024-12-13 DOI: 10.3329/bjp.v19i3.76379

Yo Zhang, Hongmei Zhang, Shanshan Yang, Xiu-Feng Gao, Wenhao Guo

{"title":"Prednisone inhibits osteosarcoma cell by regulating the Wnt/β-catenin signaling pathway","authors":"Yo Zhang, Hongmei Zhang, Shanshan Yang, Xiu-Feng Gao, Wenhao Guo","doi":"10.3329/bjp.v19i3.76379","DOIUrl":"https://doi.org/10.3329/bjp.v19i3.76379","url":null,"abstract":"Prednisone, a synthetic glucocorticoid, possesses a moderate duration of action in the human body. To investigate the effect of prednisone on osteosarcoma cells in vitro, CCK8, wound healing, Transwell assays, flow-cytometry, western blot, and ELISA were used to measure cell proliferation, cell migration, cell invasion, cell cycle, cell apoptosis, and the key proteins in Wnt/β-catenin pathway. The results showed prednisone could inhibit the proliferation in osteosarcoma cells and exhibit lower proliferation toxicity towards normal bone cells compared to cisplatin by 17.5% (p<0.001). Cell apoptosis was increased by 6.6 and 7.4%, cell migration was decreased to 62.6 and –23.9%, G0/G1 phase cells were increased by 33.0 and 17.9%, and S phase cells were reduced by 29.6 and 18.0% (all p<0.001) in Mg63 and Saos-2 cells respectively after prednisone treatment. The expressions of β-catenin, cMYC, cyclin D1, and MMP7 were also reduced. In conclusion, prednisone inhibits osteosarcoma cells by regulating the Wnt/β-catenin signaling pathway.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":"19 3","pages":"73-80"},"PeriodicalIF":0.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://banglajol.info/index.php/BJP/article/download/76379/51132","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147889628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Catalpol alleviates the lipopolysaccharide-induced inflammatory response of BV2 cells 梓醇可减轻脂多糖诱导的BV2细胞炎症反应

4区医学

Bangladesh Journal of Pharmacology Pub Date : 2024-12-13 DOI: 10.3329/bjp.v19i3.76556

Sijie Liu, Jiayi Zhu, Hao Huang, Jiaxiang Xu, Biao Zhang, Yi Luo

{"title":"Catalpol alleviates the lipopolysaccharide-induced inflammatory response of BV2 cells","authors":"Sijie Liu, Jiayi Zhu, Hao Huang, Jiaxiang Xu, Biao Zhang, Yi Luo","doi":"10.3329/bjp.v19i3.76556","DOIUrl":"https://doi.org/10.3329/bjp.v19i3.76556","url":null,"abstract":"This study aimed to investigate the effect of catalpol on the inflammatory response of murine microglial cell line (BV2 cells) induced by lipopolysaccharide. Cell proliferation activity was detected by CCK-8 assay. The morphology of BV2 was observed by an optical microscope. The inflammatory factors interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α) were detected by enzyme‐linked immunosorbent assay (ELISA). Reactive oxygen species (ROS) were detected by flow cytometry. Induced nitric oxide synthase (iNOS) level was detected by immunofluorescence. The results showed that 5 μg/mL catalpol did not effect the proliferation of BV2 cells, while 10 μg/mL catalpol significantly decreased the viability of BV2 cells. Then the experiment was carried out with 5 μg/mL catalpol. Catalpol can improve the morphology of lipopolysaccharide-induced BV2 cells, decrease the level of inflammatory factors, and reduce the production of iNOS and ROS. Therefore, catalpol can inhibit the lipopolysaccharide-induced activation of BV2 cells and has anti-inflammatory effects.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":"19 3","pages":"88-94"},"PeriodicalIF":0.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://banglajol.info/index.php/BJP/article/download/76556/51134","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147910207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Total flavonoids of Abelmoschus manihot ameliorate lipid deposition in HK-2 cells by inhibiting fatty acid uptake mediated by CD36 马尼沙鼠总黄酮通过抑制CD36介导的脂肪酸摄取改善HK-2细胞脂质沉积

4区医学

Bangladesh Journal of Pharmacology Pub Date : 2024-12-13 DOI: 10.3329/bjp.v19i3.77820

Xiaofang Wang, Chenquan Tang, Yi Jiang, Xue Yi, Enchao Zhou

引用次数: 0

Ginsenoside Rb1 ameliorates neutrophil extracellular traps-induced vascular endothelial damage by suppressing apoptosis 人参皂苷Rb1通过抑制细胞凋亡改善中性粒细胞胞外诱捕诱导的血管内皮损伤

4区医学

Bangladesh Journal of Pharmacology Pub Date : 2024-12-13 DOI: 10.3329/bjp.v19i3.76490

Biao Zhang, Junkai Zhao, Jianing Yin, J Chen, Lingqi Xu

引用次数: 0

RU.521 protects against neutrophil extracellular traps-induced vascular endothelial injury by inhibiting cGAS/STING pathway RU.521通过抑制cGAS/STING通路对中性粒细胞胞外陷阱诱导的血管内皮损伤具有保护作用

4区医学

Bangladesh Journal of Pharmacology Pub Date : 2024-06-15 DOI: 10.3329/bjp.v19i2.72930

Biao Zhang, Zaojun Fang, Ying Lin, Qian Wu, J Chen

{"title":"RU.521 protects against neutrophil extracellular traps-induced vascular endothelial injury by inhibiting cGAS/STING pathway","authors":"Biao Zhang, Zaojun Fang, Ying Lin, Qian Wu, J Chen","doi":"10.3329/bjp.v19i2.72930","DOIUrl":"https://doi.org/10.3329/bjp.v19i2.72930","url":null,"abstract":"This study aims to investigate the effect of RU.521 on improving vascular endothelial injury in 14 acute lung injury patients. Serum myeloperoxidase, double-stranded DNA (dsDNA), IFN-β, and TNF-α levels of patients were detected by ELISA. Neutrophil extracellular traps were used to stimulate human venous endothelial cells (HUVEC). cGAS/STING pathway protein was detected by western blot. The protective effect of RU.521 on HUVEC was evaluated by CCK-8. The effects of RU.521 on cGAS/STING pathway expression were detected by PCR and western blot. The levels of myeloperoxidase and (dsDNA) in acute lung injury patients were significantly increased (p<0.05). The expression level of IFN-β in acute lung injury patients was significantly increased (p<0.05). The expression of cGAS and STING proteins in HUVEC cells was significantly increased (p<0.05), and the expression of IFN-β was significantly increased (p<0.05). RU.521 can ameliorate vascular endothelial injury. RU.521 inhibited cGAS/SRING mRNA and protein expression (p<0.05). Thus, neutrophil extracellular traps release dsDNA activates the cGAS/STING pathway-induced vascular endothelial injury in acute lung injury patients. RU.521 protects vascular endothelial injury by inhibiting the cGAS/STING pathway.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":"19 2","pages":"59-64"},"PeriodicalIF":0.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://banglajol.info/index.php/BJP/article/download/72930/48955","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147891383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Berberine triggers necroptosis of cervical H8 cells by activating receptor-interacting protein kinase 1 小檗碱通过激活受体相互作用蛋白激酶1触发宫颈H8细胞坏死

4区医学

Bangladesh Journal of Pharmacology Pub Date : 2024-03-14 DOI: 10.3329/bjp.v19i1.70889

Mengting Wang, Huixian Chen, Qian Wu, Yan Huang, Yuzhen Zhou

引用次数: 2

Ethanol extract of Poria cocos induces apoptosis and differentiation in Neuro-2a neuroblastoma cells 茯苓乙醇提取物诱导神经母细胞瘤细胞凋亡和分化

4区医学

Bangladesh Journal of Pharmacology Pub Date : 2022-11-19 DOI: 10.3329/bjp.v17i4.57734

Xia Jiang, Jie Xie, Zhaotun Hu, Xiaoliang Xiang

引用次数: 2