不同生物胺的同时检测和定量

IF 0.9 4区医学 Q4 PHARMACOLOGY & PHARMACY

Bangladesh Journal of Pharmacology Pub Date : 2022-09-06 DOI:10.3329/bjp.v17i3.60650

Vishal Kumar, Ashutosh Bahuguna, Myunghee Kim

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引用次数: 1

摘要

生物胺是一组低分子量含氮有机化合物，在细胞增殖、分化和信号转导中具有重要的生理作用。此外，某些生物胺是检测人类神经内分泌和心血管疾病的重要生物标志物。除此之外，食物中丰富的生物胺被认为是一种毒素和抗营养元素，对健康有多种影响。该视觉实验展示了提取生物胺所需的所有关键步骤，随后进行衍生化和高效液相色谱分析，以连续检测和定量生物胺，如组胺、尸胺、色胺、胍丁胺、腐胺、2-苯基乙胺、精胺、酪胺和亚精胺。

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Simultaneous detection and quantification of different biogenic amines

Biogenic amines are a group of low molecular weight nitrogenous organic compounds that have an important physiological role in cell proliferation, differentiation, and signal transduction. Also, certain biogenic amines act as an important biomarker for the detection of neuroendocrine and cardiovascular disorders in humans. Besides this, the abundance of biogenic amines in food is recognized as a toxin and anti-nutritional element that have several health implications. This visual experiment demonstrates all the critical steps required for the extraction of biogenic amines followed by derivatization and high performance liquid chromatography analysis for the successive detection and quantification of biogenic amines such as histamine, cadaverine, tryptamine, agmatine, putrescine, 2-phenylethylamine, spermine, tyramine, and spermidine.

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来源期刊

Bangladesh Journal of Pharmacology PHARMACOLOGY & PHARMACY-

CiteScore

1.50

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is an open access, video component, peer-reviewed journal of the Bangladesh Pharmacological Society (BDPS). A scholarly publication is defined ''open access'' when there are no financial, legal or technical barriers to accessing it. Anyone can read, download, copy, distribute, print or use it in educational purpose within the legal agreements. Peer review of a manuscript means the evaluation of research work by one or more people with similar competences. It can be open or blind. We do the single-blind peer review. Our readers want to know the methodology used by the researchers which is very important to get the results. The video clip of one of the methodologies is prepared by the authors and submit it to publish.