AK-7通过细胞凋亡介导的抑制作用，增加人多形性胶质母细胞瘤细胞的DNA片段和胱天蛋白酶-3活性

IF 0.9 4区医学 Q4 PHARMACOLOGY & PHARMACY

Bangladesh Journal of Pharmacology Pub Date : 2022-06-06 DOI:10.3329/bjp.v17i2.59809

Ebru Güçlü, İlknur Çınar Ayan, H. Vural

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引用次数: 1

摘要

Sirtuins (SIRTs)是烟酰胺腺嘌呤二核苷酸(NAD+)依赖的III类组去乙酰化酶，在癌症中的作用存在争议。本研究研究了SIRT2抑制剂AK-7对U87胶质母细胞瘤多形性细胞的药理抑制作用。采用XTT法评价AK-7的细胞毒作用。AK-7处理后，测定细胞集落形成能力，并观察细胞凋亡情况。采用qRT-PCR检测细胞凋亡相关基因的表达水平。结果表明，AK-7对细胞增殖的抑制作用呈剂量和时间依赖性。AK-7处理后，U87细胞的集落形成能力受到抑制。AK-7增加细胞凋亡率、DNA断裂和caspase-3活性。qRT-PCR结果显示，凋亡相关基因表达水平显著升高。本研究揭示AK-7在多形性胶质母细胞瘤细胞中抑制细胞增殖并诱导细胞凋亡，SIRT2抑制可作为多形性胶质母细胞瘤的一种治疗方法。

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Inhibitory effect of AK-7 mediates by apoptosis, increases DNA fragmentation and caspase-3 activity in human glioblastoma multiforme cells

Sirtuins (SIRTs) which are nicotinamide adenine dinucleotide (NAD+) dependent class III histondeacetylases have a controversial role in cancer. In this study, the effect of pharmacological inhibition of AK-7, a SIRT2 inhibitor, was investigated in U87 glioblastoma multiforme cells. The cytotoxic effect of AK-7 was evaluated by XTT analysis. After AK-7 treatment, colony forming capacity of cells was determined and apoptosis was evaluated. The expression levels of apoptosis-related genes were determined by qRT-PCR. According to the results, AK-7 inhibited cell proliferation in a dose- and time-dependent manner. After AK-7 treatment, the colony forming capacity of U87 cells was suppressed. And, AK-7 increased apoptosis rate, DNA fragmentation, and caspase-3 activity. According to qRT-PCR, a significant increase was observed in expression levels of apoptosis-related genes. This study revealed that AK-7 inhibits cell proliferation and induces apoptosis in glioblastoma multiforme cells and SIRT2 inhibition can be evaluated as a therapeutic approach in glioblastoma multiforme.

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来源期刊

Bangladesh Journal of Pharmacology PHARMACOLOGY & PHARMACY-

CiteScore

1.50

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is an open access, video component, peer-reviewed journal of the Bangladesh Pharmacological Society (BDPS). A scholarly publication is defined ''open access'' when there are no financial, legal or technical barriers to accessing it. Anyone can read, download, copy, distribute, print or use it in educational purpose within the legal agreements. Peer review of a manuscript means the evaluation of research work by one or more people with similar competences. It can be open or blind. We do the single-blind peer review. Our readers want to know the methodology used by the researchers which is very important to get the results. The video clip of one of the methodologies is prepared by the authors and submit it to publish.