Assay and drug development technologies最新文献_第2页

MiR-22-3p Inhibits 5-Fluorouracil Resistance in Cholangiocarcinoma Cells Through PTEN/PI3K/AKT Axis. MiR-22-3p 通过 PTEN/PI3K/AKT 轴抑制胆管癌细胞的 5-氟尿嘧啶抗性

IF 1.6 4区医学

Assay and drug development technologies Pub Date : 2024-07-01 Epub Date: 2024-07-05 DOI: 10.1089/adt.2024.007

Ningrong Zhang, Li Zang

{"title":"MiR-22-3p Inhibits 5-Fluorouracil Resistance in Cholangiocarcinoma Cells Through PTEN/PI3K/AKT Axis.","authors":"Ningrong Zhang, Li Zang","doi":"10.1089/adt.2024.007","DOIUrl":"10.1089/adt.2024.007","url":null,"abstract":"\u0000 Cholangiocarcinoma (CCA) is a prevalent and highly lethal form of cancer globally. Although microRNAs (miRNAs) have been implicated in the advancement of CCA, their potential influence on 5-fluorouracil (5-Fu) resistance in CCA remains to be fully elucidated. Here, in this study, we investigated the impact of miR-22-3p on CCA resistance. Our investigation involved bioinformatics analysis, which revealed an association between miR-22-3p and the progression, diagnosis, and patient survival of CCA. Furthermore, we validated a notable downregulation of miR-22-3p expression in CCA cell lines. Elevated levels of miR-22-3p inhibit the activity and proliferation of 5-Fu-resistant CCA cell lines. In addition, we confirmed that phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is a target gene of miR-22-3p, and its expression correlates with the survival of CCA patients. Reduced PTEN expression enhances apoptosis in 5-Fu-resistant CCA cells. Meanwhile, we verified the existence of the miR-22-3p/PTEN/phosphatidylinositol-3 kinase (PI3K)/Protein kinase B (AKT) regulatory networks in CCA, influencing the sensitivity of CCA cells to 5-Fu. In conclusion, our findings suggest that miR-22-3p acts as a tumor suppressor. Its overexpression inhibits the PTEN/PI3K/AKT axis, promoting cell apoptosis and enhancing CCA sensitivity to 5-Fu.\u0000 ","PeriodicalId":8586,"journal":{"name":"Assay and drug development technologies","volume":" ","pages":"217-228"},"PeriodicalIF":1.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141533505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drug Repurposing Patent Applications January-March 2024. 2024 年 1-3 月的药物再利用专利申请。

IF 1.6 4区医学

Assay and drug development technologies Pub Date : 2024-07-01 Epub Date: 2024-06-11 DOI: 10.1089/adt.2024.047

Hermann A M Mucke

引用次数: 0

A Concise Review on Effect of PEGylation on the Properties of Lipid-Based Nanoparticles. PEG 化对脂基纳米粒子特性影响的简明综述。

IF 1.6 4区医学

Assay and drug development technologies Pub Date : 2024-07-01 Epub Date: 2024-06-03 DOI: 10.1089/adt.2024.015

Janesha Krishnan, Praveena Poomalai, Ashwin Ravichandran, Aishwarya Reddy, Raman Sureshkumar

引用次数: 0

Drug Repurposing Patent Applications January-March 2024. 2024 年 1-3 月的药物再利用专利申请。

IF 1.8 4区医学

Assay and drug development technologies Pub Date : 2024-06-11 DOI: 10.1089/adt.2024.047

Hermann A M Mucke

引用次数: 0

Beyond the Maze: Recent Advancements in Molecular and Cellular Tethered Drug Delivery Systems. 超越迷宫：分子和细胞系留给药系统的最新进展。

IF 1.6 4区医学

Assay and drug development technologies Pub Date : 2024-05-01 Epub Date: 2024-05-08 DOI: 10.1089/adt.2024.025

Dilpreet Singh

{"title":"Beyond the Maze: Recent Advancements in Molecular and Cellular Tethered Drug Delivery Systems.","authors":"Dilpreet Singh","doi":"10.1089/adt.2024.025","DOIUrl":"10.1089/adt.2024.025","url":null,"abstract":"\u0000 The relentless pursuit of precision medicine has catalyzed the development of molecular and cellular tethered drug delivery systems, a burgeoning field that stands to redefine the paradigms of therapeutic delivery. This review encapsulates the cutting-edge advancements within this domain, emphasizing the engineering of molecular tethers and cellular vectors designed to ferry therapeutics directly to their target sites with unparalleled specificity and efficiency. By exploiting the unique biochemical signatures of disease states, these systems promise a substantial reduction in off-target effects and an enhancement in drug bioavailability, thereby mitigating the systemic side effects that are often associated with conventional drug therapies. Through a synthesis of recent research findings, this review highlights the innovative approaches being explored in the design and application of these tethered systems, ranging from nanotechnology-based solutions to genetically engineered cellular carriers. The potential of these systems to provide targeted therapy for a wide array of diseases, including cancer, autoimmune disorders, and neurological conditions, is thoroughly examined. This abstract aims to provide a succinct overview of the current state and future prospects of molecular and cellular tethered drug delivery systems in advancing the frontiers of precision medicine.\u0000 ","PeriodicalId":8586,"journal":{"name":"Assay and drug development technologies","volume":" ","pages":"203-215"},"PeriodicalIF":1.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140875718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antidiabetic Evaluation of Kigelia pinnata Root Bark Extract in Streptozotocin-Induced Type-2 Diabetes Model of Rats. 松萝根皮提取物在链脲佐菌素诱导的 2 型糖尿病模型大鼠中的抗糖尿病评价

IF 1.8 4区医学

Assay and drug development technologies Pub Date : 2024-05-01 Epub Date: 2024-03-28 DOI: 10.1089/adt.2023.104

Ravindra Kumar, Neeraj Kumar, Satyendra Kumar Rajput, Sudhanshu Mallan, Arun Kumar, Balwant Singh Rawat, Naresh Kumar Rangra, Shamsher Singh

{"title":"Antidiabetic Evaluation of Kigelia pinnata Root Bark Extract in Streptozotocin-Induced Type-2 Diabetes Model of Rats.","authors":"Ravindra Kumar, Neeraj Kumar, Satyendra Kumar Rajput, Sudhanshu Mallan, Arun Kumar, Balwant Singh Rawat, Naresh Kumar Rangra, Shamsher Singh","doi":"10.1089/adt.2023.104","DOIUrl":"10.1089/adt.2023.104","url":null,"abstract":"Diabetes mellitus (DM) is the most common endocrine disorder characterized by increased blood glucose levels resulting from defective insulin secretion, resistance to insulin action, or both. DM is often associated with severe complications, and there is an increasing appreciation that cognitive function declines in DM. The aim of this research work was to evaluate Kigelia pinnata root bark extract in Streptozotocin (STZ)-induced type-2 diabetes. Experimental diabetes was induced by a single administration of STZ (60 mg/kg, intraperitoneal [i.p.]), immediately after the STZ administration, and all animals were fed with normal food and water. Nicotinamide was administered (120 mg/kg, i.p.) 15 min before STZ. The development of hyperglycemia was confirmed by the elevated blood glucose levels determined at fixed intervals, which was confirmed by measuring fasting blood glucose levels in rats' blood taken from the tail vein. Supplementation with ethanolic extract of K. pinnata root bark (EEKP) significantly reduced the elevated blood glucose in STZ-induced hyperglycemia in rats. EEKP significantly restored the biochemical and antioxidant defense system. On the final day of the protocol, the extract also reduced inflammatory cytokines in the blood serum.","PeriodicalId":8586,"journal":{"name":"Assay and drug development technologies","volume":" ","pages":"169-180"},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140304553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosomes: Key Players for Treatment of Cancer and Their Future Perspectives. 外泌体：癌症治疗的关键角色及其未来前景。

IF 1.6 4区医学

Assay and drug development technologies Pub Date : 2024-04-01 Epub Date: 2024-02-26 DOI: 10.1089/adt.2023.026

Reena Gupta, Jitendra Gupta, Suchismita Roy

引用次数: 0

High-Throughput Methods for the Discovery of Small Molecule Modulators of Pancreatic Beta-Cell Function and Regeneration. 发现胰腺 Beta 细胞功能和再生小分子调节剂的高通量方法。

IF 1.6 4区医学

Assay and drug development technologies Pub Date : 2024-04-01 Epub Date: 2024-03-25 DOI: 10.1089/adt.2023.119

Sean M McCarty, Martin C Clasby, Jonathan Z Sexton

{"title":"High-Throughput Methods for the Discovery of Small Molecule Modulators of Pancreatic Beta-Cell Function and Regeneration.","authors":"Sean M McCarty, Martin C Clasby, Jonathan Z Sexton","doi":"10.1089/adt.2023.119","DOIUrl":"10.1089/adt.2023.119","url":null,"abstract":"\u0000 The progression of type II diabetes (T2D) is characterized by a complex and highly variable loss of beta-cell mass, resulting in impaired insulin secretion. Many T2D drug discovery efforts aimed at discovering molecules that can protect or restore beta-cell mass and function have been developed using limited beta-cell lines and primary rodent/human pancreatic islets. Various high-throughput screening methods have been used in the context of drug discovery, including luciferase-based reporter assays, glucose-stimulated insulin secretion, and high-content screening. In this context, a cornerstone of small molecule discovery has been the use of immortalized rodent beta-cell lines. Although insightful, this usage has led to a more comprehensive understanding of rodent beta-cell proliferation pathways rather than their human counterparts. Advantages gained in enhanced physiological relevance are offered by three-dimensional (3D) primary islets and pseudoislets in contrast to monolayer cultures, but these approaches have been limited to use in low-throughput experiments. Emerging methods, such as high-throughput 3D islet imaging coupled with machine learning, aim to increase the feasibility of integrating 3D microtissue structures into high-throughput screening. This review explores the current methods used in high-throughput screening for small molecule modulators of beta-cell mass and function, a potentially pivotal strategy for diabetes drug discovery.\u0000 ","PeriodicalId":8586,"journal":{"name":"Assay and drug development technologies","volume":" ","pages":"148-159"},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11236284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140206280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drug Repurposing Patent Applications October-December 2023. 2023 年 10 月至 12 月的药物再利用专利申请。

IF 1.8 4区医学

Assay and drug development technologies Pub Date : 2024-04-01 Epub Date: 2024-03-05 DOI: 10.1089/adt.2024.011

Hermann A M Mucke

引用次数: 0

A Perspective on Various Facets of Nanoemulsions and its Commercial Utilities. 透视纳米乳剂的方方面面及其商业用途。

IF 1.8 4区医学

Assay and drug development technologies Pub Date : 2024-04-01 Epub Date: 2024-03-14 DOI: 10.1089/adt.2023.042

Isha Mishra, Raghav Mishra, Ashutosh Dubey, Prashant Kumar Dhakad

{"title":"A Perspective on Various Facets of Nanoemulsions and its Commercial Utilities.","authors":"Isha Mishra, Raghav Mishra, Ashutosh Dubey, Prashant Kumar Dhakad","doi":"10.1089/adt.2023.042","DOIUrl":"10.1089/adt.2023.042","url":null,"abstract":"\u0000 Nanotechnology is a captivating contemporary technology owing to its extensive range of potential applications. This study emphasizes nanomaterials, substances with a size <100 nm, offering better qualities than coarse particles. Nanoparticles have several advantages compared with conventional drug delivery methods, including enhanced bioavailability and a larger surface area because of their smaller particle size. These characteristics make the nanoparticles a viable clinical candidate. Controlled-release drug delivery systems and targeted drug delivery systems rely heavily on nanoparticles. Because traditional drug delivery methods fail to achieve targeted drug delivery, resulting in toxicity, low bioavailability, poor therapeutic outcomes, and so on, these drug nanoparticles excel in all these areas. Researchers are already interested in developing drug delivery systems such as niosomes, bilosomes, and dendrimers. Nanoemulsion is one of these technologies; nanoemulsions outperform traditional emulsions in terms of pharmacodynamics and pharmacokinetics. Nanoemulsion effectively surpasses the constraints of standard emulsions, primarily by offering enhanced bioavailability, reduced toxicity, improved absorption, and the potential to be used in targeted drug delivery or controlled-release drug delivery systems. This particular work explores several aspects of nanoemulsions, including their constituents, classification, techniques for preparation, criteria for assessment, commercial applications, and future prospects.\u0000 ","PeriodicalId":8586,"journal":{"name":"Assay and drug development technologies","volume":" ","pages":"97-117"},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140136418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0