Asian Pacific journal of allergy and immunology最新文献

{"title":"Oral administration of Lactobacillus delbrueckii subsp. lactis LDL557 attenuates airway inflammation and changes the gut microbiota in a Der p-sensitized mouse model of allergic asthma.","authors":"Pei-Chi Chen, Miao-Hsi Hsieh, Wei-Leng Chen, Yu-Pu Hsia, Wen-Shuo Kuo, Lawrence Shih-Hsin Wu, Ting Fan Shulhn-Der Wang, Xiao-Yu Liu, Jiu-Yao Wang, Hui-Fang Kao","doi":"10.12932/AP-200823-1672","DOIUrl":"10.12932/AP-200823-1672","url":null,"abstract":"<p><strong>Background: </strong>Atopic dermatitis (AD) is a chronic inflammatory skin disorder affecting up to 20% of children in developed countries. Although probiotics have shown promise as adjuvant treatments for AD, their mechanisms are not well understood.</p><p><strong>Objective: </strong>Building upon our previous studies, we investigated whether Lactobacillus gasseri and its moonlighting glyceraldehyde 3-phosphate dehydrogenase (GAPDH), namely LGp40, could be beneficial in AD management.</p><p><strong>Methods: </strong>In AD mouse models (SKH and C57BL/6J mice) with ovalbumin (OVA) and Dermatophagoides pteronyssinus (Der p) allergens, aligning with the \"outside-in\" and \"inside-out\" hypotheses, we administered L. gasseri orally and LGp40 intraperitoneally to investigate their protective effects. The evaluation involved measuring physiological, pathological, and immune function parameters. To delve deeper into the detailed mechanism of LGp40 protection in AD, additional assays were conducted using human skin keratinocytes (HaCaT) and monocytes (THP1) cell lines.</p><p><strong>Results: </strong>L. gasseri and LGp40 enhanced skin barrier function and increased skin moisture retention. They also led to reduced infiltration of Langerhans cells in the dermis and mitigated skewed Th2 and Th17 immune responses. Moreover, LGp40 inhibited allergen-induced keratinocyte apoptosis through the blockade of the caspase-3 cascade and reduced the NLR family pyrin domain containing 3 (NLRP3) inflammasome in macrophages. These inhibitions were achieved through the activation of the peroxisome proliferator-activated receptor gamma (PPARγ) pathway.</p><p><strong>Conclusion: </strong>The results of this study provide a novel insight into the mechanism of action of probiotics in the prevention and treatment for allergic disorders through the moonlighting GAPDH protein.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"161-170"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140851140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying the stability of a new wheat gliadin extract by protein analysis, skin tests and cell degranulation assay.","authors":"Chortip Chansangsawat, Surapon Piboonpocanun, Pisit Ubonsri, Punchama Pacharn, Witchaya Srisuwatchari, Kantima Kanchanapoomi, Nualanong Visitsunthorn, Orathai Jirapongsananuruk","doi":"10.12932/AP-010323-1553","DOIUrl":"10.12932/AP-010323-1553","url":null,"abstract":"<p><strong>Background: </strong>The commercial wheat extract for skin prick test (SPT) provides less sensitivity to predict wheat allergy, compared to in-house gliadin extracts. SPT is a preferred method to study extract stability as it is the aim of developing extract. The role of cell degranulation assay, a functional assay with the same mechanism as SPT, is not widely used to determine extract stability.</p><p><strong>Objective: </strong>To study the stability of in-house gliadin extracts stored at different periods, by using protein analysis, SPT and degranulation assay of humanized rat basophilic-leukemia (RBL-SX38) cells.</p><p><strong>Methods: </strong>Patients with a history of wheat allergy and positive SPT to wheat, were recruited. The gliadin extracts stored for 1, 6, 9, and 12 months at 2-8°C were used in SDS-PAGE, SPT and cell degranulation assay. The cell degranulation was determined by β-hexosaminidase release. AR patients.</p><p><strong>Results: </strong>Forty children were recruited. The gliadin extract stored for 9 and 12 months provided lighter protein bands than 1 and 6 months. However, the wheal diameters from SPT using extracts stored at different periods, were not significantly different (p = 0.09). There were also no significant differences of the β-hexosaminidase released using 0.1 and 1 μg/mL of gliadin extracts stored at different periods (p > 0.05). The 10 μg/mL of gliadin extracts stored at longer periods, significantly stimulated higher β-hexosaminidase release (p = 0.01). The extracts were sterile at all storage times.</p><p><strong>Conclusions: </strong>To determine the stability of in-house gliadin extracts, SPT or cell degranulation assay provided additional information to SDS-PAGE. The extracts were stable for up to 12 months.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"856-863"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10044874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validity and reliability of the Thai version of Food Allergy Quality of Life Questionnaire - Adult Form (FAQLQ-AF) and Food Allergy Independent Measure - Adult Form (FAIM-AF).","authors":"Chayapat Tangwiwat, Mongkhon Sompornrattanaphan, Torpong Thongngarm, Chamard Wongsa","doi":"10.12932/AP-060323-1559","DOIUrl":"10.12932/AP-060323-1559","url":null,"abstract":"<p><strong>Background: </strong>Food allergy affects the patient's quality of life (QoL) and leads to anxiety and depression. In addition to routine treatment, QoL evaluation should also be performed in patients with food allergies. The validated Food Allergy Quality of Life Questionnaire - Adult Form (FAQLQ-AF) and Food Allergy Independent Measure - Adult Form (FAIM-AF) have been well accepted and available in many languages.</p><p><strong>Objective: </strong>Translate FAQLQ-AF and FAIM-AF into Thai and perform reliability and validity tests in Thai adult patients with food allergies.</p><p><strong>Methods: </strong>The translation process was performed according to the ISPOR Task Force for Translation and Cultural Adaptation. Participants 18 years or older and with physician-diagnosed food allergies were included in the study. Thai versions of FAQLQ-AF and FAIM-AF were administered to participants at baseline and after two weeks. The intraclass correlation coefficient and Cronbach's α coefficient were evaluated to demonstrate both questionnaires' test-retest reliability and internal consistency.</p><p><strong>Results: </strong>The study included 104 participants. The Thai version of FAQLQ-AF and FAIM-AF demonstrated good reliability, with intraclass correlation coefficients of 0.83 (95%CI 0.76, 0.88) and 0.85 (95%CI 0.79, 0.90), respectively. The validity was excellent, with Cronbach's α coefficient of 0.91 and 0.92, respectively. Both questionnaires were moderately correlated (r = 0.69, P < 0.001), but poorly correlated with the 36-Item Short Form Survey, which is usually used to evaluate general health status.</p><p><strong>Conclusions: </strong>To evaluate the QoL in adult patients with food allergies, the Thai versions of FAQLQ-AF and FAIM-AF are valid, reliable, and more suitable than the general questionnaire.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"955-962"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49688588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental pollutants and allergic sensitization: A systematic literature review.","authors":"Eric Lopez-Retana, Zoe Gomez-Mendoza, Jaime Guerrero-Quezada, Camila Galvan-Coeto, Maria Tellez-Garcia, Ingrid Luna-Lopez, Luis Hernandez-Zarate, Ricardo Martinez-Tenopala, Carlos Gomez-Nunez, Victor González-Uribe","doi":"10.12932/AP-301224-1997","DOIUrl":"10.12932/AP-301224-1997","url":null,"abstract":"<p><strong>Background: </strong>Exposure to environmental pollutants has been associated with an increased risk of respiratory and allergic diseases.</p><p><strong>Objective: </strong>To describe the interactions between common pollutants and the immune system and their association with allergic diseases.</p><p><strong>Methods: </strong>A systematic literature review was conducted using PubMed, Clinical Key, Redalyc, MEDLINE, and SciELO for studies published between 2018 and 2024.</p><p><strong>Results: </strong>Evidence shows that pollutants such as PM2.5 , PM10 , NO2 , CO, and ozone trigger oxidative stress, inflammatory responses, and epithelial damage, facilitating allergic sensitization, asthma, rhinitis, and dermatitis.</p><p><strong>Conclusions: </strong>Exposure to environmental pollutants plays a key role in the development and exacerbation of allergic diseases, highlighting the need for preventive measures.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"1008-1022"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145278762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recurrent respiratory papillomatosis: Recent advances in HPV-6/HPV-11-targeted immunotherapy.","authors":"Wisarut Samuckkeethum, Kornkiat Snidvongs, Kiat Ruxrungtham","doi":"10.12932/AP-171225-2190","DOIUrl":"10.12932/AP-171225-2190","url":null,"abstract":"<p><p>Recurrent respiratory papillomatosis (RRP) is caused by human papillomavirus (HPV) types 6 and 11. Juvenile-onset RRP (JoRRP), typically acquired during birth, and adult-onset RRP (AoRRP), commonly associated with sexual transmission, are both predominantly caused by HPV-6, followed by HPV-11. HPV-11 infection, more frequent in children, is associated with more severe disease, increased risk of tracheobronchial and pulmonary spread, and, rarely, RRP-related mortality. Local immune tolerance within the airway mucosa is thought to impair clearance of HPV-6/11-infected cells, resulting in persistent infection and recurrent papilloma growth. Surgical debulking remains the cornerstone of management; however, JoRRP requires substantially more procedures than AoRRP (4-8 vs 1-2 per year), leading to cumulative morbidity, including vocal fold scarring, anterior commissure webbing, glottic or subglottic stenosis, and pulmonary dissemination. Defective innate immune activation and impaired HPV-specific cellular immunity contribute to viral persistence, with the papilloma microenvironment characterized by ineffective antiviral T-cell responses. To reduce surgical burden, immunotherapeutic strategies targeting HPV-6/11 antigens have been developed. Three platforms have advanced into phase 1-2 clinical trials: a gorilla adenoviral vector (gAdeno)-based therapy, a DNA plasmid vaccine, both encoding HPV-6/HPV-11 E6/E7 oncoproteins, and a Modified Vaccinia Ankara (MVA)-based bovine papillomavirus E2 vaccine. These approaches aim to elicit robust E6/E7-specific cellular immunity, particularly CD8+ T-cell responses, to overcome local immune tolerance and eradicate HPV-infected cells. The gAdeno-based therapy is the first FDA-approved immunotherapy for RRP; however, with annual treatment costs exceeding USD 300,000, ensuring equitable access remains a critical challenge.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":"43 4","pages":"984-996"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146200005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of asian carps parvalbumin reveals the divergence pattern of major fish allergen.","authors":"Judy Kin Wing Ng, Qing Xiong, Ling Shi, Christine Yee Yan Wai, Soo Kyung Shin, Fu Kiu Ao, Agnes Sze Yin Leung, Nicki Yat Hin Leung, Ting Fan Leung, Stephen Kwok Wing Tsui","doi":"10.12932/AP-200823-1673","DOIUrl":"10.12932/AP-200823-1673","url":null,"abstract":"<p><strong>Background: </strong>Asian carps, a popular freshwater fish globally, are valued for their flavor and serve as a crucial protein source, especially for infants. However, grass carp parvalbumin is highly allergenic, surpassing the allergenicity of fish like salmon and cod. The allergenic potential of parvalbumin in other Asian carps remains unknown, underscoring the need for allergen identification to improve the precision of fish allergy diagnosis and treatment.</p><p><strong>Objective: </strong>To identify all parvalbumin homologs in Asian carps and investigate the role of gene divergence in allergenic homolog formation.</p><p><strong>Methods: </strong>Three annotated genomes of Asian carp, including grass carp, black carp and bighead carp, were constructed using a hybrid assembly approach. Through sequence homology at the genomic level, all the homologs of major fish allergens were identified. Bioinformatics tools were then employed to reveal the gene structures, expression levels, and protein conformations of parvalbumin.</p><p><strong>Results: </strong>Grass carp genome analysis showed nine parvalbumin homologs, with Cid_PV2 most similar to Cten i 1. Bighead and black carp genomes had ten homologs, including potentially allergenic Mpi_PV7 and Hno_PV7. Tissue-specific expression patterns revealed alternative usage of parvalbumin homologs. Gene duplication events expanded parvalbumin copies in bony fish, with two gene clusters identified in Asian carp genomes.</p><p><strong>Conclusion: </strong>All the homologs of Asian carps' parvalbumin were accurately identified and gene divergence contributed to the formation of allergenic homologs. Together with a comprehensive gene sequence profile of carps' parvalbumin, those could be applied to achieve a more precise clinical diagnostic test.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"881-892"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140853080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of a new amino acid, rice glucose polymer-based, and commercial amino acid-based formulas on growth and protein status of infants with cow's milk protein allergy.","authors":"Narumon Densupsoontorn, Pipop Jirapinyo, Hathaichanok Rukprayoon, Supawan Kunnangja, Kwanjai Chotipanang, Punchama Pacharn, Patcharapa Thaweekul, Sira Nanthapisal","doi":"10.12932/AP-270123-1536","DOIUrl":"10.12932/AP-270123-1536","url":null,"abstract":"<p><strong>Background: </strong>Infants with cow's milk protein allergy (CMPA) are at risk for nutrient inadequacy and impaired growth.</p><p><strong>Objective: </strong>To evaluate the effect of a new amino acid-based formula (nAAF) compared with commercial amino acid-based formula (cAAF) on growth and protein status of cow's milk protein (CMP)-allergic infants and to compare their growth with those of healthy infants.</p><p><strong>Methods: </strong>Infants less than 6 months of age with CMPA were enrolled in the nAAF or cAAF groups. Healthy infants fed breast milk (BM) or infant formula (IF) were controls. They remained on their formula/milk until day 28 of the study. Anthropometric evaluation was performed at birth, day 0 and day 28 of the study and calculated to z-scores of weight-for-age (WAZ), length-for-age (LAZ) and head circumference-for-age (HAZ). Plasma amino acids, albumin, urea nitrogen, and creatinine were assessed for infants with CMPA on day 0 and day 28.</p><p><strong>Results: </strong>The nAAF and cAAF groups did not differ in increases in WAZ [regression coefficient (95%CI): 0.088 (-0.619, 0.796), p = 0.791], LAZ [0.045 (-0.789, 0.880, p = 0.909], and HAZ [-0.645 (-2.082, 0.793), p = 0.337] between day 0 and day 28. The increases in WAZ and LAZ during 28 days in the nAAF group did not differ from the controls. The changes in the blood chemistry values, except albumin, were not different between CMPA groups.</p><p><strong>Conclusions: </strong>The nAAF, similar to the cAAF, supports growth and protein status for infants with CMPA, and it might be used as a substitute for the cAAF.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"846-855"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9822544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum levels of specific IgE to cow's milk and its components as predictors of anaphylaxis in Chinese children with cow's milk allergy.","authors":"Wanting Qi, Jialing Chen, Huishuang Zheng, Wenjing Zhu, Kai Guan, Li Sha","doi":"10.12932/AP-010823-1667","DOIUrl":"10.12932/AP-010823-1667","url":null,"abstract":"<p><strong>Background: </strong>Cow's milk allergy (CMA) is one of the most common food allergies in young children. As improved diagnostic tools, allergic tests are inconsistent and limited in predicting anaphylaxis.</p><p><strong>Objective: </strong>To explore risk factors for anaphylaxis and to determine practical cut-offs for allergic tests in predicting anaphylaxis.</p><p><strong>Methods: </strong>This is a prospective cohort study. Children with IgE-mediated CMA were enrolled and divided into three groups (Group 1: non-anaphylaxis; Group 2: GRADE I anaphylaxis; Group 3: GRADE II-IV anaphylaxis that warranted epinephrine). Prick-to-prick tests (PTPs) using fresh cow's milk (CM) were performed. Serum specific IgE (sIgE) against CM and its components, including casein, alpha-lactalbumin, beta-lactoglobulin, and bovine serum albumin were measured. The 90% and 95% positive predictive value (PPV) decision points for predicting anaphylaxis were determined. Potential predictors of anaphylaxis were evaluated in logistic regression models.</p><p><strong>Results: </strong>This study included 134 CMA patients with a median age of 14.4 months. The sensitization rate to any CM component was 89%. Group 3 was more likely to be sensitized to multiple CM components and have higher sIgE levels. The 95% PPV diagnostic decision points of casein-sIgE in predicting anaphylaxis was 13.0 kUA/L. For GRADE II-IV anaphylaxis, casein-sIgE ≥ 54.9 kUA/L could provide a PPV of 88.9%. The elevated casein-sIgE level (OR 14.0, P=0.025) and complicating respiratory allergic diseases (OR 4.8, P=0.022) were independent risk factors for GRADE II-IV anaphylaxis.</p><p><strong>Conclusion: </strong>High casein-sIgE levels are strongly associated with CM anaphylaxis. Detection of casein-sIgE may offer an additional value for the prediction of CM anaphylaxis.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"828-838"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139110732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IgE-mediated fish allergy in Singaporean children.","authors":"Lynette Liling Tan, May Ping Lee, Wenyin Loh, Anne Goh, Si Hui Goh, Kok Wee Chong","doi":"10.12932/AP-250722-1417","DOIUrl":"10.12932/AP-250722-1417","url":null,"abstract":"<p><strong>Background: </strong>Fish is one of the common causes of food allergy and there is limited literature about fish allergy in Singapore.</p><p><strong>Objective: </strong>We aimed to describe the demographics, clinical features, and natural history of children with IgE-mediated fish allergy.</p><p><strong>Methods: </strong>A retrospective review was conducted for children diagnosed with fish allergy in a tertiary pediatric hospital in Singapore between 2015 and 2020.</p><p><strong>Results: </strong>The diagnosis of fish allergy was made in 108 patients based on a convincing history of IgE-mediated allergic reaction and a positive skin prick test. The median age at first reaction was 12 months (range 6-168) with most reacting on first ingestion (62.0%). The most common fish causing reactions were threadfin (48.1%), salmon (33.3%) and cod (31.5%). Majority presented with cutaneous symptoms (97.2%). Anaphylaxis occurred in 6.5%. Five were mono-sensitized (4.6%), 77 were oligo-sensitized (71.3%) and 26 were polysensitized (24.1%). Most can tolerate another species of fish (75.9%), most commonly salmon (37.0%), tuna (24.1%) and cod (22.2%). Median duration of follow up was 24 months (range 0-176). Twenty-eight out of 108 children (25.9%) acquired natural tolerance to index fish at a median age of 60 months (range 18-159).</p><p><strong>Conclusions: </strong>Most children with fish allergy can tolerate at least one other species of fish and resolution of fish allergy is possible. Thus, it is important to follow-up with an allergist to evaluate which fish species can be included in their diet to avoid unnecessary dietary restrictions.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"776-782"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10694829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral immunotherapy directs systemic transcriptomic changes in children with hen's egg allergy.","authors":"Sehun Jang, Ji Hwan Moon, Hosu Kim, Soyoung Jeong, Sanghee Shin, Jeongmin Song, Jung Ho Lee, Hyun Seung Choi, Christine Suh-Yun Joh, Yoonyeol Lee, Yeong Hee Kim, Min Hee Lee, Hyun Je Kim, Jihyun Kim","doi":"10.12932/AP-011124-1965","DOIUrl":"10.12932/AP-011124-1965","url":null,"abstract":"<p><strong>Background: </strong>Hen's egg (HE) is a major food allergen in children. Oral immunotherapy (OIT) has emerged as a promising therapeutic option for hen's egg allergy (HEA), but the precise immunological mechanisms underlying HE-OIT are not fully understood.</p><p><strong>Objective: </strong>We aimed to investigate the systemic immune phenotype in children with HEA and to examine transcriptomic changes during HE-OIT.</p><p><strong>Methods: </strong>We enrolled 16 children, aged between 3 and 12 years, diagnosed with HEA (median age, 4.5 years). Peripheral blood mononuclear cells were collected before the initiation of HE-OIT and after the completion of the build-up phase. The transcriptomics of the samples were analyzed using single-cell RNA sequencing.</p><p><strong>Results: </strong>All eight patients (8/8) whose blood samples were collected after the build-up phase achieved desensitization to 60 g of boiled HE white (6.0 g of HE proteins). Following the OIT build-up phase, significant reductions in total CD4+ T cells and early activated CD4+ T cell were observed (P = 0.001 and 0.045, respectively), while the frequencies of late activated CD4+ T cells and fully activated CD8+ T cells were increased (P = 0.019 and 0.038, respectively). Clonal analysis revealed proliferation within the late activated CD8+ T cell subset following OIT, indicative of the exhausted state of CD8+ T cells. Additionally, the population of regulatory T cells with abundant IKZF2 expression was significantly increased after the OIT build-up phase.</p><p><strong>Conclusions: </strong>HE-OIT was associated with systemic immune cell transcriptomic changes, suggesting that its efficacy derives from these immune alterations.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"793-810"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}