Asian Pacific journal of allergy and immunology最新文献_第6页

Production of anti-human cannabinoid receptor 2 (CB2) monoclonal antibody using non-viral vector induced human CB2 expressing myeloma as an immunogen. 以非病毒载体诱导的人 CB2 表达骨髓瘤为免疫原，生产抗人大麻素受体 2（CB2）单克隆抗体。

IF 1.9 4区医学

Asian Pacific journal of allergy and immunology Pub Date : 2026-03-01 DOI: 10.12932/AP-020723-1646

Jindaphun Kanyaruck, Takheaw Nuchjira, Putpim Chaochetdhapada, Laopajon Witida, Kotemul Kamonporn, Chaiwut Ratthakorn, Pata Supansa, Kasinrerk Watchara

{"title":"Production of anti-human cannabinoid receptor 2 (CB2) monoclonal antibody using non-viral vector induced human CB2 expressing myeloma as an immunogen.","authors":"Jindaphun Kanyaruck, Takheaw Nuchjira, Putpim Chaochetdhapada, Laopajon Witida, Kotemul Kamonporn, Chaiwut Ratthakorn, Pata Supansa, Kasinrerk Watchara","doi":"10.12932/AP-020723-1646","DOIUrl":"10.12932/AP-020723-1646","url":null,"abstract":"Background: Cannabinoid receptor 2 (CB2) of the cannabinoid system is predominantly expressed on immune cells and involved in a diverse range of immune functions. However, the role of CB2 in immunoregulation is still controversial. One of the challenges in the detailed characterization and functional study of CB2 is the lack of CB2-specific monoclonal antibodies (mAbs).Objective: We aimed to produce mAbs against a native form of human CB2 using human CB2 expressing mouse myeloma cells as immunogens.Methods: Non-viral vector expression system was used to generate stable human CB2-expressing mouse myeloma cells and were utilized as an immunogen for mouse immunization. Hybridoma technique was employed in the production of mAbs. The produced mAbs were verified by flow cytometry and western blotting.Results: Using a non-viral vector expression system, myeloma clones, which stable expressed human CB2, were generated and used as immunogen for antibody production. Following mouse immunization process, the anti-CB2 polyclonal antibodies were induced. By hybridoma technique, a mAb against CB2 could be generated. This mAb reacted to CB2-expressing THP-1 cells, but not to non-CB2-expressing SH-SY5Y cells. By western blotting, the generated anti-CB2 mAb reacted with a 42 kDa protein presented in lysates of CB2-expressing THP-1 cells, but not with non-CB2-expressing SH-SY5Y cell lysates.Conclusion: A new approach using human CB2 expressing myeloma cells as immunogen for production of anti-CB2 mAb was developed. The generated anti-human CB2 mAb is regarded as a valuable tool for CB2 characterization. Moreover, the developed technique can be applied to produce other antibodies of interest.","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"217-229"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139110730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TNFAIP3 regulates inflammatory arthritis through the differentiation of monocytes into macrophages. TNFAIP3通过单核细胞向巨噬细胞的分化调节炎性关节炎。

IF 1.9 4区医学

Asian Pacific journal of allergy and immunology Pub Date : 2026-03-01 DOI: 10.12932/AP-090924-1921

Lu Zhang, Wanlan Jiang, Biqing Zhang, Ting Xu, Shiliang Zhou, Mingyuan Cai, Jinyun Chen, Min Wu

{"title":"TNFAIP3 regulates inflammatory arthritis through the differentiation of monocytes into macrophages.","authors":"Lu Zhang, Wanlan Jiang, Biqing Zhang, Ting Xu, Shiliang Zhou, Mingyuan Cai, Jinyun Chen, Min Wu","doi":"10.12932/AP-090924-1921","DOIUrl":"10.12932/AP-090924-1921","url":null,"abstract":"Background: Rheumatoid arthritis (RA) is a disease characterized by synovitis. The synovium of RA patients is rich in macrophages, which are differentiated mainly from monocytes. The susceptibility gene of RA, tumor necrosis factor-α inducible protein 3 (tnfaip3), is considered an anti-inflammatory factor. Our previous study revealed the abnormal protein expression of TNFAIP3 in monocytes from patients with RA.Objective: In the present study, we aimed to explore the role of TNFAIP3 in monocytes in RA and its potential functions.Methods: In vivo, we injected adenoviral vectors overexpressing tnfaip3 into mice with collagen-induced arthritis (CIA) (the TNFAIP3-oe group). Arthritis scores, as well as the expression of iNOS and CD206 in the synovium, were compared between the TNFAIP3-oe group and the CIA group. In vitro, we used lentivirus transfection to upregulate/downregulate the expression of tnfaip3 in THP-1 cells. The ability of these cells to migrate, secrete cytokines and differentiate into macrophages was compared.Results: Compared with that in the CIA group, arthritis in the TNFAIP3-oe group was ameliorated (p = 0.030). Moreover, the joints of these mice presented more CD206+ cells and fewer iNOS+ cells (both p < 0.001), indicating the anti-inflammatory effect of TNFAIP3 and its regulation of macrophage polarization. In vitro, the tnfaip3-depleted cells (the TNFAIP3-i group) had greater migration and differentiated into M1 macrophages, and more cells overexpressing tnfaip3 (the TNFAIP3-oe group) differentiated into M2 macrophages. Furthermore, cells in the TNFAIP3-i group showed increased secretion of the proinflammatory cytokines IL-6 and MMPs.Conclusions: Taken together, these findings suggest that TNFAIP3 in monocytes can regulate inflammatory arthritis by modulating monocyte migration, differentiation, and cytokine secretion.","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"17-27"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A 10-year comparative study of factors for allergic asthma and/or rhinitis in two cross-sectional surveys. 两项横断面调查中过敏性哮喘和/或鼻炎发病因素的十年对比研究。

IF 1.9 4区医学

Asian Pacific journal of allergy and immunology Pub Date : 2026-03-01 DOI: 10.12932/AP-200424-1842

Wanjun Wang, Jianhong Wang, Yan Jiang, Xiaoli Han, Guolin Tan, Jianjun Chen, Qianhui Qiu, Huabin Li, Jing Li

{"title":"A 10-year comparative study of factors for allergic asthma and/or rhinitis in two cross-sectional surveys.","authors":"Wanjun Wang, Jianhong Wang, Yan Jiang, Xiaoli Han, Guolin Tan, Jianjun Chen, Qianhui Qiu, Huabin Li, Jing Li","doi":"10.12932/AP-200424-1842","DOIUrl":"10.12932/AP-200424-1842","url":null,"abstract":"Background: The allergenic relevance of the living environment changes over the last decades is largely unknown.Objective: We aimed to compare the factors associated with asthma and/or rhinitis between 2008 and 2018.Methods: We assessed two nationally representative cross-sectional datasets in 2008 and 2018. Within the rigorous protocol, questionnaire and serum IgE measurement were conducted in 2322 and 2353 patients with allergic asthma (A) and/or rhinitis (R) respectively. Multivariate logistic regression analysis was used to examine the effect of different factors on sensitization.Results: The prevalence of sensitization increased in rhinitis alone (A-R+, 63% in 2008 vs. 67.7% in 2018, P = 0.039) and asthma with rhinitis (A+R+, 70.6% vs. 75.1%, P = 0.014). The common factors for sensitization were male sex, using mattress and air conditioner, family history of rhinitis, building age > 30 years, and meat consumption. Compared with 2008, secondhand smoke was an additional risk factor for A+R- (odds ratio [OR] 2.17, 95% confidence interval [CI] 1.18-7.01) and A+R+ (OR 1.72, 95%CI 1.03-3.14), and the odds of farmland or forest for pollen and mold sensitization were higher in 2018 (OR 3.61, 95%CI 2.79-4.66, and OR 1.86, 95%CI 1.34-2.58). Eating fish was inversely associated with A-R+ (OR 0.68, 95%CI 0.52-0.91, P < 0.01), while older age also showed an inverse relationship with sensitization. The OR of age 25-44 years was higher in 2018.Conclusions: Repeated surveys showed variations in the factors affecting allergic asthma and/or rhinitis. The variable factors included age of 25-44 years, secondhand smoke, farmland, forest, and fish consumption.","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"131-143"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined effect of hygienic and polygenic risk scores in children with allergic rhinitis. 卫生和多基因风险评分在儿童变应性鼻炎中的联合作用。

IF 1.9 4区医学

Asian Pacific journal of allergy and immunology Pub Date : 2026-03-01 DOI: 10.12932/AP-070123-1524

Eom Ji Choi, Kun Baek Song, Eun Young Baek, Min Ji Park, Jisun Yoon, Sungsu Jung, Si Hyeon Lee, Mi-Jin Kang, Hea Young Oh, So-Yeon Lee, Kang Seo Park, Soo-Jong Hong

{"title":"Combined effect of hygienic and polygenic risk scores in children with allergic rhinitis.","authors":"Eom Ji Choi, Kun Baek Song, Eun Young Baek, Min Ji Park, Jisun Yoon, Sungsu Jung, Si Hyeon Lee, Mi-Jin Kang, Hea Young Oh, So-Yeon Lee, Kang Seo Park, Soo-Jong Hong","doi":"10.12932/AP-070123-1524","DOIUrl":"10.12932/AP-070123-1524","url":null,"abstract":"Background: Although the development of allergic rhinitis (AR) is associated with multiple genetic and hygienic environmental factors, previous studies have focused mostly on the effect of a single factor on the development of AR.Objective: This study aimed to investigate the combined effect of multiple genetic and hygienic environmental risk factors on AR development in school children.Methods: We conducted a cross-sectional study, comprising 1,797 children aged 9-12 years. Weighted environmental risk score (ERS) was calculated by using four hygienic environmental factors, including antibiotic use during infancy, cesarean section delivery, breast milk feeding, and having older siblings. Weighted polygenic risk score (PRS) was calculated by using four single nucleotide polymorphisms (SNPs), including interleukin-13 (rs20541), cluster of differentiation 14 (rs2569190), toll-like receptor 4 (rs1927911), and glutathione S-transferase P1 (rs1695). Multivariable logistic regression analysis was used.Results: More than three courses of antibiotic use during infancy increased the risk of current AR (adjusted odd ratio [aOR], 2.058; 95% confidence interval [CI]: 1.290-3.284). Having older siblings, especially > 2 (aOR, 0.526; 95%Cl: 0.303-0.913) had a protective effect. High ERS ( > median; aOR, 2.079; 95%Cl: 1.466-2.947) and PRS ( > median; aOR, 1.627; 95%Cl: 1.117-2.370) increased the risk of current AR independently. Furthermore, children who had both high ERS and PRS showed a higher risk of current AR (aOR, 3.176; 95%Cl: 1.787-5.645).Conclusions: Exposure to multiple hygienic risk factors during infancy increases the risk of AR in genetically susceptible children.","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"60-67"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9822545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The prevalence of IgE positivity, environmental factors, and clinical implications associated with mite species in allergic patients in Taiwan. 台湾过敏症患者的 IgE 阳性率、环境因素以及与螨虫种类相关的临床影响。

IF 1.9 4区医学

Asian Pacific journal of allergy and immunology Pub Date : 2026-03-01 DOI: 10.12932/AP-270623-1644

Chun-Hsien Hsu, Ding-Kuo Chien, Jaw-Ji Tsai, Chung-Yang Yen, En-Chih Liao

{"title":"The prevalence of IgE positivity, environmental factors, and clinical implications associated with mite species in allergic patients in Taiwan.","authors":"Chun-Hsien Hsu, Ding-Kuo Chien, Jaw-Ji Tsai, Chung-Yang Yen, En-Chih Liao","doi":"10.12932/AP-270623-1644","DOIUrl":"10.12932/AP-270623-1644","url":null,"abstract":"Background: Allergic diseases are a growing public health concern with increasing prevalence and severity. Allergens play significant roles in triggering immune responses and the development of allergic reactions.Objective: Investigate the presence and clinical significance of dust mites, storage mites, and predatory mite Cheyletus eruditus(Ce) in household environments.Methods: A survey of household dust was performed to determine mite occurrence and analyze influencing factors, an analysis of the correlation between mite species and allergic symptoms, and basophil activation triggered by mite allergens. Cross-reactivity between Ce and house dust mites was assessed.Results: The high appearance rate of mite species in households of Taiwan was Dermatophagoides pteronyssinus (Dp) and D. farinae(Df). Environmental factors such as pet keeping, vacuum cleaner usage, air conditioner usage, proximity to the kitchen, cleaning frequency, and protein concentration in beds were shown to influence mite prevalence. The appearance of Dp and Df significantly increased the occurrence of airway and nasal symptoms, while the presence of Ce was strongly correlated with skin symptoms. The activation of basophils and the correlation between specific IgE levels and allergic symptoms in response to Ce exposure were demonstrated. The presence of Ce was associated with elevated levels of allergens in bedding. The IgE adsorption between mite species was demonstrated suggesting cross-reactivity between the Ce and Dp was limited. Presence of Ce is associated with elevated levels of major mite allergens in beddings.Conclusion: Allergenicity of Ce was confirmed by IgE reactivity and basophil activation regarding mite infestation as a potential cause of skin-related allergy.","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"195-210"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139110734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an immunochromatographic strip test for rapid detection of a potent neutralizing anti-interferon gamma antibody in adult-onset immunodeficiency. 一种用于快速检测成人免疫缺陷中有效中和抗干扰素γ抗体的免疫层析试纸。

IF 1.9 4区医学

Asian Pacific journal of allergy and immunology Pub Date : 2026-03-01 DOI: 10.12932/AP-010523-1597

Umpa Yasamut, Weeraya Thongkum, Ekkarat Wongsawat, Chatchai Tayapiwatana

{"title":"Development of an immunochromatographic strip test for rapid detection of a potent neutralizing anti-interferon gamma antibody in adult-onset immunodeficiency.","authors":"Umpa Yasamut, Weeraya Thongkum, Ekkarat Wongsawat, Chatchai Tayapiwatana","doi":"10.12932/AP-010523-1597","DOIUrl":"10.12932/AP-010523-1597","url":null,"abstract":"Background: Adult-onset immunodeficiency (AOID) is associated with the presence of anti-interferon gamma autoantibodies (anti-IFN-γ autoAbs), which neutralizes IFN-γ signaling and leads to susceptibility to intracellular opportunistic infections. However, measuring neutralizing autoAbs is not practical for general clinical laboratories.Objective: This study aims to develop a competitive immunochromatographic (IC) strip test for detecting autoAbs that recognize the same epitope as a potent neutralizing antibody.Methods: The competitive IC strip test detecting autoAbs that recognize the same epitope as mouse anti-IFN-γ monoclonal antibody, clone B27 (B27 mAb) was fabricated and used to determine B27 epitope recognizing autoAb (B27 AAb) in AOID plasma. The competitive ELISA was used as a comparative method. AR patients.Results: The efficacy of the IC strip test was compared with competitive ELISA and found a percent positive agreement of 91.30%, a percent negative agreement of 79.31%, and a percent overall agreement of 84.62%.Conclusions: The results from the competitive IC strip test were consistent with those from competitive ELISA, indicating that the generated IC strip could detect B27 AAb in AOID plasma.","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"211-216"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10344123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phase III study of Phlai capsules in the treatment of allergic rhinitis: A randomized, double-blind, placebo-controlled trial. Phlai胶囊治疗过敏性鼻炎的III期研究：一项随机、双盲、安慰剂对照试验。

IF 1.9 4区医学

Asian Pacific journal of allergy and immunology Pub Date : 2026-03-01 DOI: 10.12932/AP-090223-1541

Minh P Hoang, Kachorn Seresirikachorn, Wirach Chitsuthipakorn, Jompol Samuthpongtorn, Kajohnkiat Prasittivatechakool, Pongsakorn Tantilipikorn, Orapan Poachanukoon, Pornthep Kasemsiri, Virat Kirtsreesakul, Jesada Kanjanaumporn, Songklot Aeumjaturapat, Supinda Chusakul, Kornkiat Snidvongs

{"title":"Phase III study of Phlai capsules in the treatment of allergic rhinitis: A randomized, double-blind, placebo-controlled trial.","authors":"Minh P Hoang, Kachorn Seresirikachorn, Wirach Chitsuthipakorn, Jompol Samuthpongtorn, Kajohnkiat Prasittivatechakool, Pongsakorn Tantilipikorn, Orapan Poachanukoon, Pornthep Kasemsiri, Virat Kirtsreesakul, Jesada Kanjanaumporn, Songklot Aeumjaturapat, Supinda Chusakul, Kornkiat Snidvongs","doi":"10.12932/AP-090223-1541","DOIUrl":"10.12932/AP-090223-1541","url":null,"abstract":"Background: Preclinical studies demonstrated anti-inflammatory effects of Zingiber montanum (J.König) Link ex Dietr.(Phlai). However, its clinical effect on allergic rhinitis (AR) is not evident.Objective: We sought to assess the efficacy and safety of Phlai for treating AR.Methods: A phase 3, randomized, double-blind, placebo-controlled study was conducted. Patients with AR were randomized into three groups and received Phlai 100 mg or Phlai 200 mg or placebo once a day for four weeks. The primary outcome was a change in the reflective total five symptom score (rT5SS). The secondary outcomes were the change in the instantaneous total five symptom score (iT5SS), the reflective individual symptom scores (rhinorrhea, nasal congestion, sneezing, itchy nose, itchy eyes), Rhinoconjunctivitis Quality of Life-36 Questionnaire (RCQ-36) score, peak nasal inspiratory flow (PNIF), and adverse events.Results: Two hundred and sixty-two patients were enrolled. Compared with placebo, Phlai 100 mg improved rT5SS [adjusted mean difference (aMD) -0.62; 95%CI -1.22, -0.03; p = 0.039], rhinorrhea (aMD -0.19; 95%CI -0.37, 0.002; p = 0.048), itchy nose (aMD -0.24; 95%CI -0.43, -0.05; p = 0.011), and itchy eyes (aMD -0.19; 95%CI -0.36, -0.02; p = 0.033) at week 4. Nasal obstruction, sneezing, iT5SS, overall RCQ-36 score, PNIF did not reach statistical significance. Phlai 200 mg did not bring additional benefits compared to 100 mg. Adverse events were similar among groups.Conclusions: Phlai was safe. At four weeks, there were small improvements in rT5SS, together with the individual symptoms of rhinorrhea, itchy nose, and itchy eyes.","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"28-38"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9969459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extrachromosomal circular DNA (eccDNA) in immune regulation and autoimmunity: From mechanisms to clinical applications. 染色体外环状DNA在免疫调节和自身免疫中的作用：从机制到临床应用。

IF 2.1 4区医学

Asian Pacific journal of allergy and immunology Pub Date : 2026-03-01 DOI: 10.12932/AP-180725-2123

Ho Bao Chau Le, Kollawat Somsri, Thansita Bhunyakarnjanarat, Chatsuree Suksamai, Siripong Wimon, Huy Thanh Do, Supadtra Pedcharat, Nattiya Hirankarn, Asada Leelahavanichkul

{"title":"Extrachromosomal circular DNA (eccDNA) in immune regulation and autoimmunity: From mechanisms to clinical applications.","authors":"Ho Bao Chau Le, Kollawat Somsri, Thansita Bhunyakarnjanarat, Chatsuree Suksamai, Siripong Wimon, Huy Thanh Do, Supadtra Pedcharat, Nattiya Hirankarn, Asada Leelahavanichkul","doi":"10.12932/AP-180725-2123","DOIUrl":"10.12932/AP-180725-2123","url":null,"abstract":"Extrachromosomal circular DNA (eccDNA) has transitioned from genomic curiosity to a pivotal regulator at the intersection of genomics and immunology. This review synthesizes recent advances in eccDNA biology, elucidating its integral roles in both innate and adaptive immunity and its pathogenic contributions to autoimmune diseases. eccDNA arises from diverse genomic events, including DNA damage repair, replication stress, and chromothripsis. In innate immunity, it functions as a potent damage-associated molecular pattern (DAMP), activating cytosolic sensors like cGAS-STING and AIM2 to drive type I interferon and pro-inflammatory cytokine responses. In the adaptive immune system, eccDNA is not merely a byproduct of processes such as V(D)J recombination (e.g., TRECs, KRECs); it also acts as an active modulator regulating immune gene expression via enhancer-like activity, influencing antigen presentation, and shaping T and B cell development. Critically, aberrant eccDNA accumulation is implicated in the pathogenesis of autoimmune disorders such as systemic lupus erythematosus and rheumatoid arthritis. Here, plasma eccDNA levels not only correlate with clinical disease activity indices but also track with therapeutic response, positioning eccDNA as a powerful non-invasive biomarker. Targeting eccDNA biogenesis or its sensing pathways thus represents a promising therapeutic frontier for restoring immune homeostasis. Future research integrating single-cell omics and longitudinal profiling is poised to dissect cell-specific functions and unlock the full clinical potential of eccDNA. Collectively, these findings establish eccDNA as both a functional regulator and a diagnostic tool, offering novel insights into the fundamental interplay between genomic integrity and immune function.","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":"44 1","pages":"1-16"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147716137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlations among visual analog scales, total nasal symptom scores, and peak nasal inspiratory flow in children with perennial allergic rhinitis. 常年性过敏性鼻炎患儿的视觉模拟量表、鼻腔症状总分和鼻腔吸气流量峰值之间的相关性。

IF 1.9 4区医学

Asian Pacific journal of allergy and immunology Pub Date : 2026-03-01 DOI: 10.12932/AP-030124-1757

Ongon Boonnijasin, Kantima Kanchanapoomi, Witchaya Srisuwatchari, Punchama Pacharn, Nualanong Visitsunthorn, Orathai Jirapongsananuruk

{"title":"Correlations among visual analog scales, total nasal symptom scores, and peak nasal inspiratory flow in children with perennial allergic rhinitis.","authors":"Ongon Boonnijasin, Kantima Kanchanapoomi, Witchaya Srisuwatchari, Punchama Pacharn, Nualanong Visitsunthorn, Orathai Jirapongsananuruk","doi":"10.12932/AP-030124-1757","DOIUrl":"10.12932/AP-030124-1757","url":null,"abstract":"Background: Visual analog scale (VAS) correlates well with total nasal symptom score (TNSS) but negatively correlates with peak nasal inspiratory flow (PNIF) in adults with allergic rhinitis (AR). Small children may not rate VAS properly and parents usually help assess their child's symptoms. Data on the correlations among parent-assessed VAS (P-VAS), VAS, TNSS, and PNIF in children with AR was limited.Objective: To assess correlations among P-VAS, VAS, TNSS, and PNIF in children and adolescents with perennial AR (PAR).Methods: Patients with PAR aged 6-18 years and their parents were instructed to record daily VAS, TNSS, PNIF, and P-VAS in an electronic diary for 8 weeks.Results: 2387 records from 46 patients (56.5% male) were obtained. VAS and P-VAS showed a strong correlation (rs = 0.82, p < 0.001). Moderate correlations were found between VAS vs TNSS (rs = 0.53, p < 0.001) and between P-VAS vs TNSS (rs = 0.48, p < 0.001). There was a weak negative correlation between PNIF vs VAS, PNIF vs TNSS, and PNIF vs P-VAS (rs = -0.20, rs = -0.22, rs = -0.18, p < 0.001 respectively). In addition, a weak negative correlation was found between nasal congestion and PNIF (rs = -0.26, p < 0.001). The overall inter-rater agreement between VAS and TNSS was fair (Kappa = 0.37, p < 0.001). Higher inter-rater agreement was found in moderate-severe than in the mild PAR group (Kappa = 0.50 vs 0.17) and in adolescents than in the children group (Kappa = 0.44 vs 0.26).Conclusion: In small children, P-VAS was a reliable tool to assess nasal symptoms. Both subjective and objective measurements provided complementary information for symptom monitoring in patients with AR.","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"68-74"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cost-effectiveness of salbutamol via metered-dose inhaler with spacers versus nebulizers in children with asthma exacerbation in middle income country. 中等收入国家哮喘加重儿童使用沙丁胺醇计量吸入器与雾化器的成本效益比较。

IF 1.9 4区医学

Asian Pacific journal of allergy and immunology Pub Date : 2026-03-01 DOI: 10.12932/AP-160624-1872

Jittawat Witnalakorn, Aroonwan Preutthipan, Oraluck Pattanaprateep, Wiparat Manuyakorn

{"title":"Cost-effectiveness of salbutamol via metered-dose inhaler with spacers versus nebulizers in children with asthma exacerbation in middle income country.","authors":"Jittawat Witnalakorn, Aroonwan Preutthipan, Oraluck Pattanaprateep, Wiparat Manuyakorn","doi":"10.12932/AP-160624-1872","DOIUrl":"10.12932/AP-160624-1872","url":null,"abstract":"Background: Previous studies have demonstrated that salbutamol administration via metered-dose inhaler with spacer (MDI-S) is as effective as using a jet nebulizer (NEB) for treating children experiencing asthma exacerbation. However, a paucity of research focuses on the direct medical costs associated with each mode of salbutamol administration for asthma exacerbation.Objective: This study aims to compare the effectiveness and direct medical costs of salbutamol administration via MDI-S versus NEB.Methods: A retrospective cohort study was conducted on the medical records of children under 18 years old presenting with mild to moderate asthma exacerbation. Clinical responses to salbutamol administration were assessed using the Ramathibodi Pediatrics Asthma Scores. The costs and clinical outcomes (i.e., Asthma score and hospitalization averted) were compared using the Incremental Cost-Effectiveness Ratio (ICER) from a hospital perspective.Results: The study included 95 medical records from 72 children, with 33 records of MDI-S and 62 records of NEB. Both the MDI-S and NEB groups showed significant reductions in asthma scores post-treatment. Children with moderate asthma exacerbation treated with MDI-S had a lower hospitalization rate than those treated with NEB (20% vs 57.5%, p = 0.034). The cost-effectiveness analysis indicated that the MDI-S group incurred lower costs and was considered cost-saving compared to the NEB group, with an ICER of -4.60 US dollars per one-point improvement in asthma score and -20.07 US dollars per hospitalization averted.Conclusions: Salbutamol administration via MDI-S offers clinical effectiveness comparable to NEB and is more cost-effective.","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"96-102"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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