Asian Pacific journal of allergy and immunology最新文献

{"title":"Recurrent chronic spontaneous urticaria in a tropical country: Clinical characteristics and associated factors.","authors":"Pichaya Limphoka, Sukhum Jiamton, Leena Chularojanamontri, Kanokvalai Kulthanan, Papapit Tuchinda","doi":"10.12932/AP-261124-1984","DOIUrl":"https://doi.org/10.12932/AP-261124-1984","url":null,"abstract":"<p><strong>Background: </strong>Limited data exist regarding recurrent chronic spontaneous urticaria (RCSU) following complete disease remission.</p><p><strong>Objective: </strong>This study investigated the clinical characteristics and factors associated with RCSU.</p><p><strong>Methods: </strong>We retrospectively reviewed data from chronic spontaneous urticaria (CSU) patients who actively visited the Urticaria Center of Reference and Excellence, Siriraj Hospital, between January 2021 and December 2023. Medical records were analyzed through May 2024. The RCSU was defined as a new CSU episode occurring after a 6-month symptom-free period without treatment.</p><p><strong>Results: </strong>Among 179 CSU patients, 19 (10.6%) developed RCSU. These patients had a mean age of 40 (SD 14.9) years, with a female predominance. The mean time to RCSU recurrence was 1.96 (SD 2.05) years. The UAS7 and medication scores were not significantly different between the recurrence and nonrecurrence groups (P = 0.675, P = 0.77). Multivariate analysis revealed that a shorter disease remission time from the first episode onset (< 3 years) was significantly associated with RCSU (odds ratio 5.13, 95%CI 1.83-14.29; P = 0.002).</p><p><strong>Conclusions: </strong>The RCSU rate was 10.6%. The time to disease remission from the first episode onset significantly associated with RCSU. Several clinical characteristics may corelate with the recurrence: younger age at onset, the presence of angioedema, chronic inducible urticaria comorbidity, systemic corticosteroid use, and positive antinuclear antibody status.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of patients with suspected vaccine allergies in Singapore.","authors":"Zi Teng Chai, Jia Yi Goh, Karen Jui Lin Choo, Kheng Yong Ong, Vivian Tan, Chiara Jiamin Chong, Chaw Su Naing, Haur Yueh Lee","doi":"10.12932/AP-140724-1891","DOIUrl":"https://doi.org/10.12932/AP-140724-1891","url":null,"abstract":"<p><strong>Background: </strong>Hypersensitivity reaction to vaccines has been reported to occur in 5 per 100,000 doses. Although hypersensitivity reactions can occur to either the active vaccine component or other components such as excipients, outcome data from skin testing and provocation remains limited.</p><p><strong>Objective: </strong>To evaluate the role of skin testing and vaccine provocation in patients with an allergy label to vaccine.</p><p><strong>Methods: </strong>This is a single centre, prospective study between March 2021 and November 2021 of adults with known allergy to non-COVID vaccine. All participants underwent skin prick testing (SPT) and intradermal testing (IDT) to vaccine and excipients. A subset of patients with negative skin testing underwent graded vaccine provocation.</p><p><strong>Results: </strong>A total of 264 adults were evaluated. The most common index vaccine reactions were nonspecific rash (47.7%), angioedema (32.2%) and itch (25.0%). All patients had negative SPT to vaccines and excipients. Thirty patients (11.4%) had positive IDT to Hepatitis A, Hepatitis B, Human Papilloma Virus (HPV), Influenza, Measles-Mumps-Rubella (MMR), Pneumococcal, Rabies, Diphteria, Tetanus and Pertussis (DTaP). Out of 234 patients with negative IDT, 32 patients (12.1%) underwent vaccine provocation. Three patients (9.4%) developed reaction to influenza and MMR vaccine. One patient required systemic corticosteroids, one required antihistamine, and another patient did not require any treatment. None required admission or attendance at emergency department.</p><p><strong>Conclusions: </strong>The majority of allergy labels to vaccine are inaccurate based on low skin test positivity and low reaction rates on vaccine provocation. Vaccine provocation is safe. Excipients are unlikely to be the main cause of hypersensitivity reactions in vaccines.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IgE-sensitization and cross-reactivity of Der f 23 and Der p 23 in Korean patients with allergy.","authors":"Myung-Hee Yi, Tai-Soon Yong, Chung-Ryul Kim, Kyoung Yong Jeong, Ju Yeong Kim","doi":"10.12932/AP-120924-1924","DOIUrl":"https://doi.org/10.12932/AP-120924-1924","url":null,"abstract":"<p><strong>Background: </strong>House dust mites (HDM) are a major source of allergens, and more than 30 HDM allergens have been identified to date. Recently, Der p 23 was reported to be a major allergen that is related to the severity of allergic responses.</p><p><strong>Objective: </strong>This study aimed to characterize IgE-sensitization and cross-reactivity between Der f 23 and Der p 23 in Korean patients with allergy.</p><p><strong>Methods: </strong>We produced recombinant Der f 23 and Der p 23 using a yeast Pichia expression system. The IgE binding activity of Der f 23 and Der p 23 was evaluated by enzyme-linked immunosorbent assay (ELISA) using sera from 194 Korean HDM-sensitized patients. The cross-reactivity between Der f 23 and Der p 23 was then assayed using competitive ELISA.</p><p><strong>Results: </strong>Among the 194 HDM-allergic patients, IgE reactivity to rDer f 23 and rDer p 23 was observed in 45.36% (88/194) and 43.81% (85/194) of the samples, respectively. Competitive ELISA with pooled serum from 10 patients revealed that rDer p 23 inhibited 86.1% of the rDer f 23 IgE reactivity and rDer f 23 inhibited 61.1% of the rDer p 23 IgE reactivity.</p><p><strong>Conclusions: </strong>Group 23 HDM allergens, Der f 23 and Der p 23, show moderate sensitization, with 45.36% and 43.81% of Korean patients with allergy reacting to them, respectively. Significant IgE cross-reactivity was observed between the two allergens. These findings can facilitate the development of component-resolved diagnosis and allergen-specific immunotherapy in the future.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunoglobulin E binding capacity to various types of Korean soy sauces among children sensitized to wheat and soybean.","authors":"Jeongmin Lee, Purevsan Gantulga, Kyunguk Jeong, Sanghwa Youm, Sooyoung Lee","doi":"10.12932/AP-110924-1922","DOIUrl":"https://doi.org/10.12932/AP-110924-1922","url":null,"abstract":"<p><strong>Background: </strong>Soy sauce (SS) is made from soy or a mix of soybean and wheat proteins. With the rise in global consumption of Korean SS, understanding the allergenicity of its different types is essential.</p><p><strong>Objective: </strong>This study is the first to evaluate whether the immunoglobulin E binding capacity (IgE+) in children sensitized to soybeans or wheat varies by SS types.</p><p><strong>Methods: </strong>Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and an enzyme-linked immunosorbent assay (ELISA) using pooled and individual sera were performed with three SS types. Inhibition ELISA was performed using SS concentrate, extracts of wheat, and soybean.</p><p><strong>Results: </strong>Guk-SS showed very faint protein bands, on SDS-PAGE. ELISA (pooled sera, n = 36) indicated that all groups had the lowest IgE+ to Guk-SS. In individual ELISA, patients sensitized to both wheat and soybeans (WS; n = 4) showed significantly higher IgE+ to all SS types (Yangjo > Jin > Guk) than those sensitized to wheat (W; n = 3) or Soy (S; n = 3) alone. The W group had IgE+ similar to the control group for Guk and Jin-SS, while the S group showed slightly higher IgE+ to Yangjo-SS. When soybean was used as an inhibitor, the IgE+ to Yangjo-SS and Jin-SS was inhibited by more than 50% (Yangjo > Jin). In contrast, wheat exhibited only subtle inhibition of both SS (< 20%). Converse showed similar results.</p><p><strong>Conclusions: </strong>For pediatric patients sensitized to wheat, it is suggested that Korean SS be carefully introduced at home. For patients sensitized to soybeans, it is safest to try Guk-SS before attempting other types.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of budesonide nasal irrigation on Hypothalamic-Pituitary-Adrenal Axis in patients with chronic rhinosinusitis post endoscopic sinus surgery: A prospective study.","authors":"Jesada Kanjanaumporn, Sorranut Thaweboon, Songklot Aeumjaturapat, Kornkiat Snidvongs, Supinda Chusakul, Kachorn Seresirikachorn, Lalita Prathanee","doi":"10.12932/AP-121124-1968","DOIUrl":"https://doi.org/10.12932/AP-121124-1968","url":null,"abstract":"<p><strong>Background: </strong>Corticosteroids added to high volume saline nasal irrigation have been introduced as a more effective method of delivering corticosteroids to the sinuses than nasal sprays. However, information regarding the effect of this intervention on the hypothalamic-pituitary-adrenal (HPA) axis is still limited.</p><p><strong>Objective: </strong>To evaluate the safety of long-term corticosteroid (6 months) nasal irrigation in patients with chronic rhinosinusitis (CRS) post endoscopic sinus surgery.</p><p><strong>Methods: </strong>Seventeen patients with CRS were included. After undergoing endoscopic sinus surgery, the patients were prescribed budesonide nasal irrigations (250 ml via squeeze bottle) twice daily (1 mg/day) for six months. The serum morning cortisol levels of these patients were then evaluated at 3 and 6 months post-operatively.</p><p><strong>Results: </strong>Median serum morning cortisol levels were 10.5 mcg% at pre-operative baseline; 10.3 mcg% at 3 months; and 11.2 mcg% at 6 months on post-operative follow-up. There were no significant changes in the serum morning cortisol levels (P value = 0.71 and 0.63 respectively). Three of 17 patients (17.65%) had mildly abnormal serum morning cortisol levels (4, 4.3 and 4.9 mcg%) at 3 months. However, these levels were within a normal range at 6 months.</p><p><strong>Conclusions: </strong>Serum morning cortisol levels were not significantly changed after usage of budesonide nasal irrigation for 6 months.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of pre-existing immunity on humoral and cellular responses to CoronaVac in SARS-CoV-2 variants: A focus on common human Coronaviruses.","authors":"Nawamin Pinpathomrat, Bunya Seeyankem, Ratchanon Sophonmanee, Jomkwan Ongarj, Smonrapat Surasombatpattana","doi":"10.12932/AP-201124-1979","DOIUrl":"https://doi.org/10.12932/AP-201124-1979","url":null,"abstract":"<p><strong>Background: </strong>The global COVID-19 pandemic, caused by SARS-CoV-2, has highlighted the importance of understanding immune responses elicited by vaccines.</p><p><strong>Objective: </strong>This study evaluated antibody and T cell responses to the inactivated CoronaVac vaccine, as well as the role of pre-existing immunity to common human coronaviruses (HCoVs) in shaping vaccine-induced immunity.</p><p><strong>Methods: </strong>We enrolled 64 participants (17 males and 47 females) and measured IgG levels against HCoVs before and after vaccination. T cell responses were analysed by stimulating peripheral blood mononuclear cells (PBMCs) with wild-type, Delta, and Omicron spike peptides.</p><p><strong>Results: </strong>We found pre-existing antibodies against HCoV-229E, HCoV-HKU1, HCoV-NL63, and HCoV-OC43 were present before vaccination. Notably, a positive correlation was observed between pre-existing antibodies to HCoV-229E and HCoV-HKU1 and anti-RBD IgG levels post-vaccination. Pre-existing CD4+ T cell responses were observed for the wild-type strain before vaccination, with a significant reduction in IFN-γ secretion after Delta re-stimulation and partial restoration after Omicron re-stimulation. IL-4 production by CD4+ T cells was significantly reduced upon re-stimulation with Delta and Omicron compared to wild-type. CD8+ T cells again showed a reduction of IL-4 production after Delta re-stimulation compared to the original strain.</p><p><strong>Conclusions: </strong>This work demonstrate that CoronaVac induces robust humoral and cellular immune responses, though variant-specific responses vary. Pre-existing immunity to certain HCoVs may influence vaccine-induced antibody responses, underscoring the importance of monitoring immunity to emerging SARS-CoV-2 variants and informing future vaccine design.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Denatonium benzoate promotes IgE-mediated mast cell degranulation and ovalbumin-induced allergic diarrhea in BALB/c mice.","authors":"Ju Liu, Huaping Xu, Shaotong Feng, Xiaoyun Shi, Xin Li, Jiexin Zou, Linbo Shi","doi":"10.12932/AP-250424-1845","DOIUrl":"https://doi.org/10.12932/AP-250424-1845","url":null,"abstract":"<p><strong>Background: </strong>Denatonium benzoate (DB), one of the most bitter compounds known to man, is used for alcohol denaturation. Some reports have demonstrated that asthmatic symptoms are associated with DB exposure, but the possible links between DB and immunoglobulin E (IgE)-mediated allergy susceptibility have not yet been examined.</p><p><strong>Objective: </strong>This study investigated the effects of DB on in vitro IgE-mediated mast cell degranulation and food allergy in BALB/c mice.</p><p><strong>Methods: </strong>IgE-sensitized rat RBL-2H3 cells (a basophilic leukemia mast cell line) and human KU812 cells (a basophilic cell line) and mice with ovalbumin (OVA)-induced allergy were treated with DB. Histamine release, calcium ion (Ca²⁺) influx, phosphorylated spleen tyrosine kinase (p-Lyn) and phosphorylated phospholipase C-γ (p-PLCγ) levels, OVA-specific IgE, anaphylactic symptoms, and the cell-surface expression of the high-affinity IgE receptor α-subunit (FcεRIα) on mast cells were evaluated.</p><p><strong>Results: </strong>DB increased histamine release, Ca²⁺ mobilization, and p-Lyn and p-PLCγ levels in IgE-mediated activated RBL-2H3 and KU812 cells, and enhanced the cell-surface expression of FcεRIα messenger RNA (mRNA). In mice, DB increased the severity of OVA-induced anaphylactic and diarrheic symptoms, the mucus thickness in the jejunum, histamine and OVA-specific IgE levels, and FcεRIα mRNA in isolated mucosal mast cells.</p><p><strong>Conclusions: </strong>Our work indicates that DB promotes IgE-mediated mast cell degranulation and OVA-induced allergic diarrhea in BALB/c mice, providing evidence that exposure to DB promotes allergy susceptibility.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of exposure to ambient air volatile organic compounds on the severity of atopic dermatitis and lag-day effect.","authors":"Hui-Wen Tseng","doi":"10.12932/AP-101124-1967","DOIUrl":"https://doi.org/10.12932/AP-101124-1967","url":null,"abstract":"<p><strong>Background: </strong>Previous studies reported positive associations between exposure to air volatile organic compounds (VOCs) and daily visits for atopic dermatitis (AD).</p><p><strong>Objective: </strong>This population-based study investigated associations between air VOCs exposure and daily hospitals visits for AD, severity subgroup (mild and severe), and lag-day effect in central-southern Taiwan.</p><p><strong>Methods: </strong>The dependent variable was AD with diagnostic codes (ICD-9-CM 691.8 and ICD-10-CM L20) retrieved from the Taiwan National Health Insurance Research Database from 2008/01/01 to 2018/12/31. Independent variables included one-day 75th-percentile value of each VOC (benzene, ethylbenzene, toluene, m-/p-xylene, o-xylene, 1,3,5-trimethylbenzene, 1,2,4-trimethylbenzene, isopentane, n-pentane, n-hexane, methylcyclohexane, and cyclohexane) and four meteorological conditions from the Taiwan Air Quality Monitoring Network Database. This was a case-crossover design with multivariable conditional logistic regression, and the adjusted odds ratios (AORs) were reported.</p><p><strong>Results: </strong>Concentrations of the 12 air VOCs significantly positively affected the total number of daily visits for AD (AOR = 1.02~1.69, P < 0.001) and subgroups of mild (AOR = 1.001~1.049, P < 0.001) and severe (AOR = 1.002~1.077, P < 0.001). The effect of air VOCs on the severe AD group was higher than that on the mild group. Values of the six VOCs on the 1st lag day (benzene: AOR = 1.16, 1,3,5-trimethylbenzene: AOR = 1.5, 1,2,4-trimethylbenzene: AOR = 1.13, isopentane: AOR = 1.07, n-pentane: AOR = 1.08, methylcyclohexane: AOR = 1.5, all P < 0.05) were significantly positively associated with the number of daily visits for AD.</p><p><strong>Conclusions: </strong>Exposure to the 12 air VOCs on the visit days increased the risks of daily visits for AD in total and severity subgroup. The effects of six certain VOCs on the 1st lag day were significant positive.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of daily aspirin intake on platelet-associated factors and aggregation in nonsteroidal anti-inflammatory drug exacerbated respiratory disease: A cross-sectional study.","authors":"Selcan Genc, Basak Ezgi Sarac, Ozge Can Bostan, Gulseren Tuncay, Hayriye Akel Bilgic, Baran Erman, Umit Sahiner, Gul Karakaya, Ali Fuat Kalyoncu, Ebru Damadoglu, Cagatay Karaaslan","doi":"10.12932/AP-261024-1959","DOIUrl":"https://doi.org/10.12932/AP-261024-1959","url":null,"abstract":"<p><strong>Background: </strong>Nonsteroidal anti-inflammatory drug (NSAID)-exacerbated respiratory disease (N-ERD) is a clinical syndrome characterized by chronic rhinosinusitis with nasal polyposis (CRSwNP), adult-onset asthma and hypersensitivity to NSAIDs. Long-term aspirin treatment after desensitization (ATAD) is used for clinical improvement in N-ERD patients. However, information on the potential effect of ATAD on the platelet-neutrophil aggregates (PNA) level in N-ERD patients is highly limited.</p><p><strong>Objective: </strong>This study aimed to explore the impact of PNA on the pathogenesis of N-ERD and the potential effect of ATAD on N-ERD patient profiles from a platelet point-of-view.</p><p><strong>Methods: </strong>Sixty-one individuals were enrolled, including 16 N-ERD patients with ATAD (ATAD+), 15 N-ERD patients without ATAD (ATAD-), 15 aspirin-tolerant asthma (ATA) patients, and 15 healthy controls (HCs). Lipid mediators classical in N-ERD, including urinary-LTE4 (uLTE4), prostaglandin-D2 (PGD2), and prostaglandin-E2 (PGE2) were assessed by ELISA. Platelet activation was estimated based on expression levels of sP-selectin, CD40L, Platelet Factor-4 (PF4), RANTES, Thromboxane-A2 (TXA2), PAF, 12-HETE in plasma levels by ELISA; and PNA percentage by flow cytometry.</p><p><strong>Results: </strong>ATAD+; 12-HETE, and PF4 levels were remarkably low, while higher levels were determined in ATAD- and ATA groups. ATAD+; uLTE4 levels were positively correlated with 12-HETE. Another positive correlation was detected between sP-selectin and 12-HETE in ATAD-. Compared to HCs, it was found that among all N-ERD patients, significant increase in PNA.</p><p><strong>Conclusions: </strong>Plasma levels of PGE2, PF4, and 12-HETE appear to be affected by aspirin treatment. We believe that 12-HETE could play a significant role in the N-ERD pathogenesis by contributing to platelet activation.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of anti-elastin antibodies in a mouse model of asthma.","authors":"Tomoshige Wakayama, Kumiya Sugiyama, Shingo Tokita, Hajime Arifuku, Naotatsu Otsuji, Kei Sugitate, Takayoshi Owada, Kenya Koyama, Hirokuni Hirata, Masafumi Arima, Yasutsugu Fukushima","doi":"10.12932/AP-020221-1053","DOIUrl":"10.12932/AP-020221-1053","url":null,"abstract":"<p><strong>Background: </strong>The role of anti-elastin antibody (Ab) in the lung is unclear, although they may be involved in chronic obstructive pulmonary disease (COPD). Recently, increased anti-elastin Ab levels were reported in asthma.</p><p><strong>Objective: </strong>To elucidate the role of anti-elastin Ab in asthma, we created a murine asthma model. Anti-elastin Ab in the airway was neutralized by intratracheal administration of elastin peptide, and the inhibitory effects of anti-elastin Ab on airway remodeling were evaluated.</p><p><strong>Methods: </strong>BALB/c mice were immunized with ovalbumin (OVA) on days 0 and 14. After immunization, the mice received booster OVA via inhalation twice per week for 9 weeks, and bronchoalveolar lavage fluid (BALF) and lung tissues were evaluated.</p><p><strong>Results: </strong>In lung tissues, airway remodeling occurred after 9 weeks of OVA sensitization. Peak levels of anti-elastin Ab and eosinophils in BALF were detected after 3 weeks of OVA sensitization. Anti-elastin Ab and eosinophil levels in BALF were significantly reduced after 3 weeks by the neutralization of anti-elastin Ab. Peak transforming growth factor-β1 levels in BALF were detected at 3 weeks after OVA sensitization and were significantly reduced by the neutralization of anti-elastin Ab. Airway remodeling in lung tissues was also significantly inhibited by the neutralization of anti-elastin Ab.</p><p><strong>Conclusions: </strong>In our murine asthma model, anti-elastin Ab was recruited to the airway by OVA-induced allergic inflammation. Airway remodeling was inhibited by the neutralization of anti-elastin Ab. Anti-elastin Ab may contribute to the progression of airway remodeling.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"56-64"},"PeriodicalIF":2.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39434837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}