Asian Pacific journal of allergy and immunology最新文献

{"title":"Recurrence rate and risk factors of recurrent anaphylaxis: A ten-year retrospective cohort study.","authors":"Suwannee Uthaisangsook, Nadda Padsee, Sagoontee Inkate","doi":"10.12932/AP-130325-2047","DOIUrl":"10.12932/AP-130325-2047","url":null,"abstract":"<p><strong>Background: </strong>Few studies have investigated the risk factors for recurrent anaphylaxis. Identifying these factors may help patients implement preventive measures.</p><p><strong>Objective: </strong>To determine the rate and risk factors for recurrent anaphylaxis, assess the time to recurrence, and compare the characteristics, triggers, and clinical manifestations between recurrent and non-recurrent cases.</p><p><strong>Methods: </strong>A retrospective cohort study was conducted at Naresuan University Hospital from March 2011 to February 2021, using medical records of patients with ICD-10-confirmed anaphylaxis. Risk factors for recurrence were analyzed using Cox proportional hazards regression model.</p><p><strong>Results: </strong>A total of 439 anaphylactic episodes were identified in 381 patients (49 children, 332 adults). Of these, 42 patients (11.2%) experienced 58 recurrent episodes (7/49 [14.3%] children, 35/332 [10.6%] adults). Food and medications were the most and second most common triggers. The median time to recurrence was 9.9 months (IQR: 3.1-18.8), while the median follow-up duration for non-recurrent cases was 41.8 months (IQR: 23.8-61.8). The recurrent anaphylaxis rate was 4.1 events per 100 person-years. Statistically significant risk factors included a history of food, a history of insect, a history of drug allergies, chest discomfort, and severe anaphylaxis (HR [95%CI]: 3.31 [1.50-7.29], p = 0.003; 4.96 [1.47-16.82], p = 0.010; 5.87 [2.64-13.07], p < 0.001; 2.43 [1.19-4.99], p = 0.015; and 2.29 [1.07-4.88], p = 0.033, respectively). Conversely, palpitations were associated with a lower risk of recurrence (HR 0.11 [0.01-0.86], p = 0.036).</p><p><strong>Conclusions: </strong>Identifying risk factors in anaphylaxis patients enhances medical care and aids in preventing recurrence.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral immunotherapy directs systemic transcriptomic changes in children with hen's egg allergy.","authors":"Sehun Jang, Ji Hwan Moon, Hosu Kim, Soyoung Jeong, Sanghee Shin, Jeongmin Song, Jung Ho Lee, Hyun Seung Choi, Christine Suh-Yun Joh, Yoonyeol Lee, Yeong Hee Kim, Min Hee Lee, Hyun Je Kim, Jihyun Kim, Kangmo Ahn","doi":"10.12932/AP-011124-1965","DOIUrl":"10.12932/AP-011124-1965","url":null,"abstract":"<p><strong>Background: </strong>Hen's egg (HE) is a major food allergen in children. Oral immunotherapy (OIT) has emerged as a promising therapeutic option for hen's egg allergy (HEA), but the precise immunological mechanisms underlying HE-OIT are not fully understood.</p><p><strong>Objective: </strong>We aimed to investigate the systemic immune phenotype in children with HEA and to examine transcriptomic changes during HE-OIT.</p><p><strong>Methods: </strong>We enrolled 16 children, aged between 3 and 12 years, diagnosed with HEA (median age, 4.5 years). Peripheral blood mononuclear cells were collected before the initiation of HE-OIT and after the completion of the build-up phase. The transcriptomics of the samples were analyzed using single-cell RNA sequencing.</p><p><strong>Results: </strong>All eight patients (8/8) whose blood samples were collected after the build-up phase achieved desensitization to 60 g of boiled HE white (6.0 g of HE proteins). Following the OIT build-up phase, significant reductions in total CD4+ T cells and early activated CD4+ T cell were observed (P = 0.001 and 0.045, respectively), while the frequencies of late activated CD4+ T cells and fully activated CD8+ T cells were increased (P = 0.019 and 0.038, respectively). Clonal analysis revealed proliferation within the late activated CD8+ T cell subset following OIT, indicative of the exhausted state of CD8+ T cells. Additionally, the population of regulatory T cells with abundant IKZF2 expression was significantly increased after the OIT build-up phase.</p><p><strong>Conclusions: </strong>HE-OIT was associated with systemic immune cell transcriptomic changes, suggesting that its efficacy derives from these immune alterations.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced performances of the short-PCR coupled lateral flow assay in the detection of Candida albicans in clinical blood samples.","authors":"Oranee Srichaiyapol, Bhanubong Saiboonjan, Sawinee Ngernpimai, Chonphaksorn Ponsue, Nutcha Sa-Ingthong, Patsara Thongmee, Lumyai Wonglakorn, Chutipapa Sukkasem, Rinjong Promson Kendal, Jureerut Daduang, Ratree Tavichakorntrakool, Arpasiri Srisrattakarn, Aroonwadee Chanawong, Molin Wongwattanakul, Aroonlug Lulitanond, Patcharaporn Tippayawat","doi":"10.12932/AP-190125-2018","DOIUrl":"10.12932/AP-190125-2018","url":null,"abstract":"<p><strong>Background: </strong>Candida albicans remains the most common fungal pathogen among the species, causing candidemia. Thus, early diagnosis is indispensable in patients with severe underlying infections.</p><p><strong>Objective: </strong>To develop a short-polymerase chain reaction (short-PCR) coupled with lateral flow strip (LFS) assay for the detection of C. albicans in clinical blood samples.</p><p><strong>Methods: </strong>A short-PCR-LFS was enhanced to detect clinical isolates and clinical blood samples. The ITS2 gene of C. albicans was amplified using the modified primers-probes to produce highly specific, dual-labeled amplicons. The sensitivity and specificity of the test system were evaluated using C. albicans, Candida spp. other than C. albicans and other microbial DNAs. The test system was validated by 44 clinical isolates and 51 clinical blood samples.</p><p><strong>Results: </strong>The short-PCR-LFS revealed a high specificity for C. albicans with no cross-reactivity and a limit of detection (LOD) of 0.1 ng per 2 mL of blood and 2 CFU/mL using a direct colony as a template. The result was consistent with the validation by short-PCR agarose gel electrophoresis (AGE). The short-PCR-LFS assay showed all positives with all C. albicans relevant samples and exhibited negative for other microbial relevance samples.</p><p><strong>Conclusions: </strong>The entire process of this system provides visual detection results less than 1 h with high sensitivity, high specificity, DNA extraction-free method, and little dependence on instruments. Thus, it can be considered as a promising method for professional use to early detect and identify clinical relevance samples of C. albicans.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Delphi consensus on nebulized budesonide for adults with asthma and COPD.","authors":"Phunsup Wongsurakiat, Akrawat Rattanawongpaibul, Atikun Limsukon, Chirawat Chiewchalermsri, Kittipong Maneechotesuwan, Kittiyaporn Wiwatcharagoses, Kumpol Kornthatchapong, Narongkorn Saiphoklang, Pitsucha Sanguanwit, Pornanan Domthong, Tirachat Sewatanon, Wipa Reechaipichitkul, Theerasuk Kawamatawong","doi":"10.12932/AP-190824-1910","DOIUrl":"https://doi.org/10.12932/AP-190824-1910","url":null,"abstract":"<p><strong>Background: </strong>Inhaled corticosteroids (ICS) represent an alternative treatment option to systemic corticosteroids (SCS) in the treatment of asthma and chronic obstructive pulmonary disease (COPD); however, detailed clinical guidance on the use of nebulized ICS, such as budesonide, in the management of asthma and COPD remains scarce.</p><p><strong>Objective: </strong>To review the literature and develop Delphi consensus statements on the use of nebulized ICS for the management of asthma and COPD in adults.</p><p><strong>Methods: </strong>An expert panel of 13 respiratory physicians, comprising pulmonologists (n = 9), allergists (n = 1), and emergency department consultants (n = 3) from tertiary medical centers in Thailand, undertook a Delphi procedure with the aim of developing evidence-based consensus statements on the use of nebulized ICS in patients with asthma and COPD. Panelists used a 5-point Likert scale to score their agreement with each statement.</p><p><strong>Results: </strong>A total of 12 Delphi consensus statements pertaining to the use of nebulized ICS in the management of asthma and COPD in both acute and maintenance care were developed. The overall consensus of the panel across the 12 statements was very high (mean agreement score, 4.2-4.9/5). The panelists expressed strongest consensus agreement (84.6% strong agreement) with the following two statements: 1) inhalation devices are the cornerstone of drug delivery in patients with asthma and COPD, and 2) for adult asthma and COPD patients with severe exacerbations, nebulization is more suitable for drug delivery than a pMDI plus spacer.</p><p><strong>Conclusions: </strong>Nebulized budesonide is an effective and well tolerated treatment option for the management of asthma and COPD.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitization profiles of aeroallergens among allergic rhinitis patients in central China: A six-year real-world study.","authors":"Li Le, Luo Chunhua, Chen Hao, Wang Yin, Liu Jin, Xu Qingxiu, Zhu Rongfei","doi":"10.12932/AP-070125-2002","DOIUrl":"https://doi.org/10.12932/AP-070125-2002","url":null,"abstract":"<p><strong>Background: </strong>Environmental changes have led to shifts in allergen sensitization patterns globally.</p><p><strong>Objective: </strong>To assess changes in aeroallergen sensitization profiles among AR patients in Yichang, a city located in central China along the Yangtze River and develop an optimized allergen panel for the diagnosis of AR through specific IgE (sIgE) testing.</p><p><strong>Methods: </strong>This retrospective study encompassed all patients with suspected AR who sought care at a university hospital in Yichang between 2018 and 2023 and underwent IgE testing. Demographic and sIgE data were collected.</p><p><strong>Results: </strong>A total of 11,855 patients were included in the study, with 7,453 (62.88%) testing positive for at least one allergen. The predominant allergen identified was house dust mite (HDM), with sensitization rates of 58.65% for D. pteronyssinus and 58.63% for D. farinae. Notably, sensitization to animal dander showed a significant increase, with cat dander rising from 4.41% in 2018 to 12.50% in 2023, and dog dander from 4.41% to 10.42%. Sensitization rates for various allergens also exhibited fluctuations, with seasonal peaks observed: HDM (summer/autumn), molds (spring/autumn), and Blattella germanica (autumn/winter). A five-allergen panel (HDM, B. germanica, mixed animal dander, mixed molds) identified over 95% of sensitized patients.</p><p><strong>Conclusions: </strong>HDM remains the primary allergen among AR patients in this area, with rising sensitization to animal dander. A five-allergen panel effectively detects most sensitized patients.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TNFAIP3 regulates inflammatory arthritis through the differentiation of monocytes into macrophages.","authors":"Lu Zhang, Wanlan Jiang, Biqing Zhang, Ting Xu, Shiliang Zhou, Mingyuan Cai, Jinyun Chen, Min Wu","doi":"10.12932/AP-090924-1921","DOIUrl":"https://doi.org/10.12932/AP-090924-1921","url":null,"abstract":"<p><strong>Background: </strong>Rheumatoid arthritis (RA) is a disease characterized by synovitis. The synovium of RA patients is rich in macrophages, which are differentiated mainly from monocytes. The susceptibility gene of RA, tumor necrosis factor-α inducible protein 3 (tnfaip3), is considered an anti-inflammatory factor. Our previous study revealed the abnormal protein expression of TNFAIP3 in monocytes from patients with RA.</p><p><strong>Objective: </strong>In the present study, we aimed to explore the role of TNFAIP3 in monocytes in RA and its potential functions.</p><p><strong>Methods: </strong>In vivo, we injected adenoviral vectors overexpressing tnfaip3 into mice with collagen-induced arthritis (CIA) (the TNFAIP3-oe group). Arthritis scores, as well as the expression of iNOS and CD206 in the synovium, were compared between the TNFAIP3-oe group and the CIA group. In vitro, we used lentivirus transfection to upregulate/downregulate the expression of tnfaip3 in THP-1 cells. The ability of these cells to migrate, secrete cytokines and differentiate into macrophages was compared.</p><p><strong>Results: </strong>Compared with that in the CIA group, arthritis in the TNFAIP3-oe group was ameliorated (p = 0.030). Moreover, the joints of these mice presented more CD206+ cells and fewer iNOS+ cells (both p < 0.001), indicating the anti-inflammatory effect of TNFAIP3 and its regulation of macrophage polarization. In vitro, the tnfaip3-depleted cells (the TNFAIP3-i group) had greater migration and differentiated into M1 macrophages, and more cells overexpressing tnfaip3 (the TNFAIP3-oe group) differentiated into M2 macrophages. Furthermore, cells in the TNFAIP3-i group showed increased secretion of the proinflammatory cytokines IL-6 and MMPs.</p><p><strong>Conclusions: </strong>Taken together, these findings suggest that TNFAIP3 in monocytes can regulate inflammatory arthritis by modulating monocyte migration, differentiation, and cytokine secretion.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allergenicity potential of protein extract from freshwater and saltwater fish based on heat stability and antibody-binding frequency.","authors":"Atika H Falihah, Zulfi Azizah, Bayu Ba Santoso, Ika P Sari, Muhammad Na Sahid","doi":"10.12932/AP-221024-1958","DOIUrl":"https://doi.org/10.12932/AP-221024-1958","url":null,"abstract":"<p><strong>Background: </strong>Saltwater fish are associated with more allergic reactions compared to freshwater fish. However, the factors contributing to this difference remain unclear.</p><p><strong>Objective: </strong>To compare the heat stability of freshwater and saltwater fish proteins, and assess their binding affinity to allergen-specific antibodies.</p><p><strong>Methods: </strong>Protein extracts were isolated from saltwater fish-Selar crumenophthalmus, Euthynnus affinis, Ambassis urotaenia, and freshwater fish i.e., Rasbora argyrotaenia, Monopterus albus, and Poecilia reticulata. Protein extract from Penaeus monodon served as a standard allergen source. Both raw and heat-treated protein extracts were subjected to SDS-PAGE analysis. The number of protein bands, their molecular sizes, and intensities were evaluated. Protein binding frequencies to anti-tropomyosin antibodies and IgE-containing serum from allergic patients were measured using ELISA.</p><p><strong>Results: </strong>The P. monodon protein extract < 100 kDa demonstrated heat stability, while A. urotaenia proteins < 40 kDa were also heat-stable. Raw protein extracts from R. argyrotaenia and M. albus exhibited binding frequencies to anti-tropomyosin IgG of 28.18 ± 1.05% and 14.79 ± 0.91%, respectively. In saltwater fish, raw protein extracts from A. urotaenia and S. crumenophthalmus showed binding frequencies of 61.74 ± 1.87% and 34.68 ± 1.39%, respectively. Freshwater and saltwater fish heat-treated protein extracts displayed binding frequencies below 10%. All heat-treated protein samples exhibited higher binding frequencies to polyclonal IgE in patient sera compared to their raw counterparts.</p><p><strong>Conclusions: </strong>Proteins smaller than 20 kDa exhibit significant heat stability. Raw protein extracts show higher binding frequencies to monoclonal IgG against crustacean tropomyosin, while heat-treated samples have increased binding frequency to IgE-containing human serum.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recurrent chronic spontaneous urticaria in a tropical country: Clinical characteristics and associated factors.","authors":"Pichaya Limphoka, Sukhum Jiamton, Leena Chularojanamontri, Kanokvalai Kulthanan, Papapit Tuchinda","doi":"10.12932/AP-261124-1984","DOIUrl":"https://doi.org/10.12932/AP-261124-1984","url":null,"abstract":"<p><strong>Background: </strong>Limited data exist regarding recurrent chronic spontaneous urticaria (RCSU) following complete disease remission.</p><p><strong>Objective: </strong>This study investigated the clinical characteristics and factors associated with RCSU.</p><p><strong>Methods: </strong>We retrospectively reviewed data from chronic spontaneous urticaria (CSU) patients who actively visited the Urticaria Center of Reference and Excellence, Siriraj Hospital, between January 2021 and December 2023. Medical records were analyzed through May 2024. The RCSU was defined as a new CSU episode occurring after a 6-month symptom-free period without treatment.</p><p><strong>Results: </strong>Among 179 CSU patients, 19 (10.6%) developed RCSU. These patients had a mean age of 40 (SD 14.9) years, with a female predominance. The mean time to RCSU recurrence was 1.96 (SD 2.05) years. The UAS7 and medication scores were not significantly different between the recurrence and nonrecurrence groups (P = 0.675, P = 0.77). Multivariate analysis revealed that a shorter disease remission time from the first episode onset (< 3 years) was significantly associated with RCSU (odds ratio 5.13, 95%CI 1.83-14.29; P = 0.002).</p><p><strong>Conclusions: </strong>The RCSU rate was 10.6%. The time to disease remission from the first episode onset significantly associated with RCSU. Several clinical characteristics may corelate with the recurrence: younger age at onset, the presence of angioedema, chronic inducible urticaria comorbidity, systemic corticosteroid use, and positive antinuclear antibody status.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of patients with suspected vaccine allergies in Singapore.","authors":"Zi Teng Chai, Jia Yi Goh, Karen Jui Lin Choo, Kheng Yong Ong, Vivian Tan, Chiara Jiamin Chong, Chaw Su Naing, Haur Yueh Lee","doi":"10.12932/AP-140724-1891","DOIUrl":"https://doi.org/10.12932/AP-140724-1891","url":null,"abstract":"<p><strong>Background: </strong>Hypersensitivity reaction to vaccines has been reported to occur in 5 per 100,000 doses. Although hypersensitivity reactions can occur to either the active vaccine component or other components such as excipients, outcome data from skin testing and provocation remains limited.</p><p><strong>Objective: </strong>To evaluate the role of skin testing and vaccine provocation in patients with an allergy label to vaccine.</p><p><strong>Methods: </strong>This is a single centre, prospective study between March 2021 and November 2021 of adults with known allergy to non-COVID vaccine. All participants underwent skin prick testing (SPT) and intradermal testing (IDT) to vaccine and excipients. A subset of patients with negative skin testing underwent graded vaccine provocation.</p><p><strong>Results: </strong>A total of 264 adults were evaluated. The most common index vaccine reactions were nonspecific rash (47.7%), angioedema (32.2%) and itch (25.0%). All patients had negative SPT to vaccines and excipients. Thirty patients (11.4%) had positive IDT to Hepatitis A, Hepatitis B, Human Papilloma Virus (HPV), Influenza, Measles-Mumps-Rubella (MMR), Pneumococcal, Rabies, Diphteria, Tetanus and Pertussis (DTaP). Out of 234 patients with negative IDT, 32 patients (12.1%) underwent vaccine provocation. Three patients (9.4%) developed reaction to influenza and MMR vaccine. One patient required systemic corticosteroids, one required antihistamine, and another patient did not require any treatment. None required admission or attendance at emergency department.</p><p><strong>Conclusions: </strong>The majority of allergy labels to vaccine are inaccurate based on low skin test positivity and low reaction rates on vaccine provocation. Vaccine provocation is safe. Excipients are unlikely to be the main cause of hypersensitivity reactions in vaccines.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IgE-sensitization and cross-reactivity of Der f 23 and Der p 23 in Korean patients with allergy.","authors":"Myung-Hee Yi, Tai-Soon Yong, Chung-Ryul Kim, Kyoung Yong Jeong, Ju Yeong Kim","doi":"10.12932/AP-120924-1924","DOIUrl":"https://doi.org/10.12932/AP-120924-1924","url":null,"abstract":"<p><strong>Background: </strong>House dust mites (HDM) are a major source of allergens, and more than 30 HDM allergens have been identified to date. Recently, Der p 23 was reported to be a major allergen that is related to the severity of allergic responses.</p><p><strong>Objective: </strong>This study aimed to characterize IgE-sensitization and cross-reactivity between Der f 23 and Der p 23 in Korean patients with allergy.</p><p><strong>Methods: </strong>We produced recombinant Der f 23 and Der p 23 using a yeast Pichia expression system. The IgE binding activity of Der f 23 and Der p 23 was evaluated by enzyme-linked immunosorbent assay (ELISA) using sera from 194 Korean HDM-sensitized patients. The cross-reactivity between Der f 23 and Der p 23 was then assayed using competitive ELISA.</p><p><strong>Results: </strong>Among the 194 HDM-allergic patients, IgE reactivity to rDer f 23 and rDer p 23 was observed in 45.36% (88/194) and 43.81% (85/194) of the samples, respectively. Competitive ELISA with pooled serum from 10 patients revealed that rDer p 23 inhibited 86.1% of the rDer f 23 IgE reactivity and rDer f 23 inhibited 61.1% of the rDer p 23 IgE reactivity.</p><p><strong>Conclusions: </strong>Group 23 HDM allergens, Der f 23 and Der p 23, show moderate sensitization, with 45.36% and 43.81% of Korean patients with allergy reacting to them, respectively. Significant IgE cross-reactivity was observed between the two allergens. These findings can facilitate the development of component-resolved diagnosis and allergen-specific immunotherapy in the future.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}