Artificial organs最新文献

{"title":"\"Off-The-Shelf\" Bioartificial Liver Support System Using Cryopreserved Immobilized Hepatocyte Spheroids in a Porcine Acute Liver Failure Model.","authors":"Ji-Hyun Lee, Young-A Kim, Doo-Hoon Lee, Hee-Hoon Yoon, Sanghoon Lee, Suk-Koo Lee","doi":"10.1111/aor.70034","DOIUrl":"10.1111/aor.70034","url":null,"abstract":"<p><strong>Background: </strong>We had previously established an adequate cryopreservation process for hepatocyte spheroids for the development of an off-the-shelf bioartificial liver (BAL) system. The purpose of this study is to evaluate the efficacy of a BAL system containing cryopreserved immobilized porcine hepatocyte spheroids in a porcine model of acute liver failure (ALF).</p><p><strong>Methods: </strong>ALF pigs were divided into three groups. The control group consisted of treatment-naïve pigs (n = 5), the blank group consisted of pigs that were attached to the BAL system not containing hepatocyte spheroids for 12 h (n = 5), and the BAL group consisted of pigs that were attached to the BAL containing hepatocyte spheroids for 12 h (n = 5).</p><p><strong>Results: </strong>Analysis of specific oxygen uptake rates showed that the hepatocytes sustained vigorous activity throughout the 12-h period of BAL operation. Median survival time of pigs was 24.0 h in the BAL group, 17.0 h in the blank group, and 15.0 h in the control group. The BAL group showed significantly prolonged survival according to Kaplan-Meier survival analysis compared to the blank group (p = 0.04) and control group (p = 0.04). Serum ammonia levels were significantly increased in the blank group (p < 0.01) and control group (p < 0.01), compared to the BAL group during the treatment period of the porcine ALF models. Prothrombin time (PT) was significantly lower in the BAL group compared to the blank group (p = 0.04) and control group (p = 0.01).</p><p><strong>Conclusions: </strong>A BAL system with a bioreactor containing cryopreserved immobilized hepatocyte spheroids showed effective clearance of serum ammonia, preservation of renal function, and improved survival in a porcine model of ALF.</p>","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":"404-411"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145421103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-Long Polymyxin B Hemoperfusion and Its Effect on Vasopressor Dosage and Organ Dysfunction in Patients With Septic Shock Requiring High-Dose Norepinephrine: A Post Hoc Analysis of a Prospective Cohort Study.","authors":"Kyohei Miyamoto, Yu Kawazoe, Noriko Miyagawa, Hitoshi Yamamura, Yoshinori Ohta, Takuya Kimura, Yukitoshi Toyoda, Michihito Kyo, Tetsuya Sato, Masashi Kinjo, Masaki Takahashi, Junichi Maruyama, Hiroshi Matsuura, Kazunori Fukushima, Takeshi Morimoto","doi":"10.1111/aor.70025","DOIUrl":"10.1111/aor.70025","url":null,"abstract":"<p><strong>Background: </strong>Prolonged duration of polymyxin B hemoperfusion (PMX-HP) for septic shock is not widely investigated.</p><p><strong>Methods: </strong>We used BEAT-SHOCK, a prospective registry including 309 adult patients with septic shock requiring high-dose norepinephrine (≥ 0.2 μg/kg/min). Our post hoc analysis included 82 patients that underwent PMX-HP, dichotomized into either the ultra-long PMX-HP group (first session duration ≥ 12 h; n = 53) or the non-ultra-long PMX-HP group (< 12 h; n = 29). The primary outcomes were changes in vasopressor/inotrope dosage, represented by vasoactive-inotropic score (VIS), and sequential organ failure assessment (SOFA) score from baseline to day 3.</p><p><strong>Results: </strong>The median durations of the first PMX-HP session in the ultra-long and non-ultra-long PMX-HP groups were 1290 and 358 min, respectively. Their median baseline VIS was 38.3 (IQR 26.0-49.5) in the ultra-long group, 38.1 (IQR 30.9-55.6) in the non-ultra-long PMX-HP group. The median baseline SOFA score was 11 (IQR 9-13) in both groups. The changes in these scores from baseline to day 3 did not differ between them (adjusted difference -3.8 [95% confidence interval -9.0 to 11.3] for VIS and -0.0 [95% confidence interval -1.5 to 1.5] for SOFA score). The 90-day mortality rate was 24.7% in the ultra-long PMX-HP group and 21.1% in the non-ultra-long PMX-HP group (adjusted hazard ratio 1.35; 95% confidence interval 0.49-3.69).</p><p><strong>Conclusions: </strong>Ultra-prolonged PMX-HP for ≥ 12 h was not associated with a greater reduction in vasopressor/inotrope dosage or improvement in organ dysfunction on day 3 than PMX-HP < 12 h in our patients with septic shock without endotoxin monitoring.</p><p><strong>Trial registration: </strong>UMIN Clinical Trial Registry on 1 November 2019 (registration no. UMIN000038302).</p>","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":"458-467"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and Preclinical Evidence on a Novel Percutaneous Pulsatile Ventricular Assist Device (PulseCath): A Scoping Review.","authors":"Viviana Teresa Agosta, Jacopo D'Andria Ursoleo, Corrado D'Andria, Alice Bottussi, Fabrizio Monaco","doi":"10.1111/aor.70022","DOIUrl":"10.1111/aor.70022","url":null,"abstract":"<p><strong>Background: </strong>In recent decades, temporary mechanical circulatory support (tMCS) devices have garnered increasing interest for the treatment of cardiogenic shock. PulseCath-a pulsatile ventricular assist device-may address a critical unmet need within the tMCS array, offering an intermediate level of support between that of intra-aortic balloon pump (IABP) and more invasive tMCS devices (e.g., Impella and ECMO).</p><p><strong>Methods: </strong>We conducted a systematic search of PubMed/MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials, Web of Science, Scopus, and Google Scholar databases to retrieve available clinical and preclinical in vivo data regarding the implantation of PulseCath.</p><p><strong>Results: </strong>Five preclinical in vivo studies (38 animal models) and 16 clinical studies (386 patients) were identified and retrieved. When implanted in humans, PulseCath was mostly employed with a \"pre-emptive\" strategy, particularly in the context of high-risk percutaneous coronary interventions. However, cases reporting its \"bail-out\" deployment in instances of hemodynamic instability were also identified.</p><p><strong>Conclusions: </strong>While the underreporting of adverse events and of PulseCath use as a bailout strategy across literature constrains a definitive evaluation of the device, preliminary findings suggest an apparent ease of use. Therefore, further research is warranted to better delineate which specific patient population would most likely benefit from the implantation of such a device. As such, the integration of underlying pathophysiological data, individualized risk assessment, and more robust clinical studies may potentially expand its use.</p>","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":"355-370"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145243407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-Incision Anterolateral Thoracotomy for LVAD Implantation: A Novel Minimally Invasive Approach.","authors":"Ali Saad Merzah, Günes Dogan, Alina Zubarevich, Stefan Rümke, Bastian Schmack, Ezin Deniz, Jasmin Hanke, Arjang Ruhparwar, Jan D Schmitto, Alexander Weymann","doi":"10.1111/aor.70070","DOIUrl":"10.1111/aor.70070","url":null,"abstract":"<p><p>A novel single-incision anterolateral thoracotomy technique enables complete HeartMate 3 implantation without sternotomy, minimizing surgical trauma and accelerating recovery. This minimally invasive approach is feasible, safe, and may represent a new standard in advanced heart failure surgery.</p>","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":"474-477"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13090743/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145653209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydraulic In Vitro Characterization of MR-Conditional Blood Pumps.","authors":"Dominik T Schulte, Marcel Renggli, Henning Richter, Francesca Del Chicca, Michael Hofmann, Martin O Schmiady, Marianne Schmid Daners","doi":"10.1111/aor.70041","DOIUrl":"10.1111/aor.70041","url":null,"abstract":"<p><strong>Background: </strong>Many cardiac surgical procedures use cardiopulmonary bypass (CPB), which in neonates requires the heart-lung machine (HLM) to be positioned close to the patient due to their small circulating blood volume. The absence of an MR-compatible blood pump to be used in CPB remains a key challenge for studying perioperative brain injury mechanisms in this high-risk group. This study aims to take the first step toward MR-conditional HLMs by developing hardware, verifying pump hydraulics, and ensuring MR compatibility.</p><p><strong>Methods: </strong>This study presents three MR-conditional blood pump prototypes: a roller pump, a non-occlusive roller pump, and a centrifugal pump. MR compatibility was assessed by monitoring for imaging interference during scanning. Hydraulic performance was evaluated with pressure-flow diagrams using a mock circulation test bench.</p><p><strong>Results: </strong>None of the prototypes interfered with MR imaging, and although SNR was reduced by -8.43% ± 7.96%, image quality remained sufficient for reliable assessment of relevant brain regions. Roller and non-occlusive pumps maintained stable flow across pressure heads, with reductions of -4.46 ± 8.02 and -119.33 ± 18.22 mL/min, respectively. The centrifugal pump exhibited pressure-dependent performance (slope -2.21 ± 0.45 mmHg/[L/min]). All pumps generated non-pulsatile flow (SHE < 1000 erg/cm³).</p><p><strong>Conclusion: </strong>All three pumps meet basic MR-conditionality and flow requirements, supporting their potential for use in MRI-guided studies during neonatal cardiac surgery.</p>","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":"393-403"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13090745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145534326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Colloid Preservation Solution for Ex Situ Hypothermic Perfusion of the Heart: A Preclinical Pilot Study.","authors":"Simon Dang Van, Dorothée Brunet, Benoit Decante, Linda Grimaud, Laurent Loufrani, Julien Guihaire","doi":"10.1111/aor.70060","DOIUrl":"10.1111/aor.70060","url":null,"abstract":"<p><strong>Background: </strong>Hypothermic ex situ heart graft perfusion using acellular preservation solution is a promising technique for heart transplantation.</p><p><strong>Methods: </strong>We investigated the cardioprotective effects of a novel preservation solution, IGL-2 (Institut Georges Lopez, France), in a porcine model of hypothermic ex situ heart perfusion over 4 h, following either beating-heart procurement or donation after circulatory death.</p><p><strong>Results: </strong>Coronary endothelial injury (assessed by Endothelin-1) and oxidative stress markers (assessed by nitrates and hydrogen peroxide) were evaluated through repeated perfusate sampling. Cardiac power output was measured after ex situ warm reperfusion in working mode.</p><p><strong>Conclusion: </strong>Preservation with IGL-2 was associated with reduced endothelial injury and oxidative stress, resulting in improved myocardial recovery following blood reperfusion.</p>","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":"468-473"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145653234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Percutaneous Versus Surgical Cannulation for Femoro-Femoral Venoarterial Extracorporeal Membrane Oxygenation: A Retrospective Cohort Study on Cannulation-Related Complications.","authors":"Axel Dimberg, Magnus Dalén, Anders Franco-Cereceda, Thomas Fux","doi":"10.1111/aor.70061","DOIUrl":"10.1111/aor.70061","url":null,"abstract":"<p><strong>Background: </strong>Cannulation for peripheral veno-arterial extracorporeal membrane oxygenation (V-A ECMO) can lead to severe local complications. This study evaluated site-specific complications between percutaneous and surgical cannulation techniques for femoro-femoral V-A ECMO, focusing on site bleeding, infection, and limb ischemia.</p><p><strong>Methods: </strong>We conducted a single-center retrospective cohort study of adult patients who received femoro-femoral V-A ECMO either by percutaneous or surgical technique at a tertiary center. Cannulation-site complications were assessed from cannulation to post-decannulation. Risk factors for site bleeding, infection, and limb ischemia within 90 days of cannulation were analyzed using logistic regression.</p><p><strong>Results: </strong>Among 384 patients (22.4% female), 181 (47.1%) underwent percutaneous, and 203 (52.9%) underwent surgical cannulation. Percutaneous cannulation was associated with significantly fewer patients experiencing site bleeding (29.3% vs. 40.9%, p = 0.02) and infection (8.3% vs. 31.0%, p < 0.001), with no significant difference in limb ischemia (11.6% vs. 15.3%, p = 0.29). 90-day survival was similar between groups (43.6% vs. 49.8%, p = 0.81). Multivariable logistic regression identified surgical cannulation as an independent risk factor for site bleeding (OR 2.39, 95% CI 1.43-3.98; p < 0.001) and infection (OR 5.47; 95% CI 2.47-12.12; p < 0.001). Limb ischemia was not significantly associated with the cannulation technique but with two other modifiable factors at cannulation: absence of distal perfusion catheterization and larger arterial cannula size.</p><p><strong>Conclusion: </strong>Percutaneous cannulation was associated with significantly fewer patients experiencing site bleeding and infection compared to surgical cannulation. Limb ischemia was not associated with the cannulation technique but was influenced by two other modifiable factors at cannulation: distal perfusion catheterization and arterial cannula size.</p>","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":"440-448"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13090744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145572891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends in Left Ventricular Assist Device Utilization and Survival Outcomes Following the Donor Allocation Policy Change.","authors":"Christopher D Pritting, Daler Rahimov, Matthew P Weber, Eugene Storozynsky, Howard J Eisen, J Eduardo Rame, Rene J Alvarez, Keshava Rajagopal, John W Entwistle, Vakhtang Tchantchaleishvili","doi":"10.1111/aor.70046","DOIUrl":"10.1111/aor.70046","url":null,"abstract":"<p><strong>Background: </strong>We sought to estimate left ventricular assist device use (LVAD) with waitlist and post-transplant survival trends in patients supported on durable LVAD in relation to the UNOS donor heart allocation policy revision.</p><p><strong>Methods: </strong>Adult patients implanted with HeartMate II LVAD, HeartWare HVAD, or HeartMate 3 LVAD, and listed for isolated heart transplantation between October 18, 2013, and June 16, 2023, were identified in the UNOS database and were stratified into pre- and post-policy revision groups. Patients on temporary circulatory support, right, or biventricular assist devices, total artificial hearts, recipients of donation after circulatory death (DCD) hearts, and those undergoing simultaneous multiorgan transplants were excluded. Waitlist and post-transplant survival were compared between the groups.</p><p><strong>Results: </strong>Policy revision was associated with a decrease in LVAD use both at the time of listing (pre: 28% vs. post: 24.5%, p < 0.01) and at the time of transplant (pre: 45.8% vs. post: 31.7%, p < 0.01). For those listed with an LVAD, more patients eventually underwent transplantation (pre: 89.6% vs. post: 94.3%, p < 0.01) following the policy change. Overall waitlist and post-transplant survival among patients supported on LVAD worsened following the policy change, both showing statistical significance (p < 0.01).</p><p><strong>Conclusion: </strong>In the post-policy era, a decline in durable LVAD utilization was observed, along with worse waitlist and post-transplant outcomes among LVAD-supported patients.</p>","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":"412-419"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145534349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Danshen-Enhanced Cardioprotective Effect of Cardioplegia on Ischemia Reperfusion Injury in a Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Model\".","authors":"","doi":"10.1111/aor.70109","DOIUrl":"10.1111/aor.70109","url":null,"abstract":"","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":"483"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ex Vivo Preservation in Vascularized Composite Allotransplantation: State of the Art, Challenges, and Perspectives.","authors":"Haïzam Oubari, Alexandre G Lellouch, Ali Mojallal, Curtis L Cetrulo, Korkut Uygun, Yanis Berkane","doi":"10.1111/aor.70068","DOIUrl":"10.1111/aor.70068","url":null,"abstract":"<p><p>Vascularized composite allotransplantation (VCA) offers unparalleled reconstructive possibilities in complex cases but remains constrained by high immunogenicity and marked susceptibility to ischemia-reperfusion injury (IRI), particularly in muscle-rich grafts. Static cold storage (SCS), the current clinical standard, preserves grafts only for short durations. In contrast, machine perfusion (MP), already transformative in solid organ transplantation, is emerging as a promising strategy for VCA. This review summarizes the main challenges of ex vivo VCA preservation and current perfusion strategies designed to overcome them. Particular attention is given to physiological and technical factors influencing graft integrity, as well as innovations in perfusate composition and protective additives that mitigate IRI and support tissue preservation. Beyond simple storage, MP platforms enable functional assessment and therapeutic interventions, including graft reconditioning and immune modulation prior to transplantation. Complementary subzero static preservation methods, such as supercooling and cryopreservation, also show promise for substantially extending preservation times. Together with advances in experimental models, these approaches are reshaping the preservation landscape. As the field evolves, MP is poised to become a cornerstone technology in VCA, improving graft quality, extending preservation duration, and enabling pre-implantation modification strategies to reduce rejection and enhance long-term outcomes.</p>","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":"334-343"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145628029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}