Artificial organs最新文献

{"title":"Comparison of a Miniaturized Implantable Two-Stage Rotodynamic Blood Pump to Equivalent Single-Stage Concepts.","authors":"Sarah Linnemeier, Bente Thamsen, Stefan Maric, Bernhard Semlitsch, Benjamin Torner, Daniel Zimpfer, Marcus Granegger","doi":"10.1111/aor.70152","DOIUrl":"https://doi.org/10.1111/aor.70152","url":null,"abstract":"<p><strong>Background: </strong>Hemocompatibility-related adverse events still limit the broader adoption of rotodynamic blood pumps. This study aims to evaluate the potential of a multistage pump design to enhance hemocompatibility in left ventricular assist devices.</p><p><strong>Methods: </strong>Computational fluid dynamic simulations were utilized to compare the pump performance and hemocompatibility of a Two-Stage Pump to two equivalent single-stage devices, designed for identical operating conditions. One single-stage design features equal impeller and volute dimensions; the other, equal specific speed and specific diameter. Our simulation setup was validated against in vitro experiments of the pump performance of the Two-Stage Pump.</p><p><strong>Results: </strong>The Two-Stage Pump achieved a substantial reduction in circumferential velocity (24% and 29% reduction). Although the two-stage configuration thereby effectively reduces shear stress, it simultaneously translates to a longer residence time due to the larger priming volume. However, because the numerical blood damage estimation weighs shear stress exponentially more than residence time, the beneficial effect of reduced shear stress partially compensates for the longer residence time. As a result, the Two-Stage Pump demonstrates a lower hemolysis index (12% and 14% reduction) compared to both single-stage designs.</p><p><strong>Conclusion: </strong>Our comparative study demonstrates that the two-stage concept offers favorable hemolysis measures compared to single-stage designs, as the reduction in peak shear stresses outweighs the disadvantage associated with prolonged residence time.</p>","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An In Vitro System for Studying Toxin Transport Across Hemodialysis Membranes.","authors":"M Torrents-Yeste, D Ramada, O E M Ter Beek, F Pel, J de Vries, B H Lentferink, K G F Gerritsen, D Stamatialis","doi":"10.1111/aor.70139","DOIUrl":"https://doi.org/10.1111/aor.70139","url":null,"abstract":"<p><strong>Background: </strong>Healthy kidneys regulate acid-base balance and electrolytes, produce hormones, and clear excess fluids and uremic toxins. Progressive kidney function loss in chronic kidney disease leads to end-stage kidney disease (ESRD), where fluid and toxin accumulation causes severe complications and can be fatal. ESRD requires renal replacement therapy; kidney transplantation is preferred; however, limited availability of donor organs makes hemodialysis (HD) the most used treatment option. In the last years, many studies had focused on development of new membranes with improved toxin removal, however, these studies implement various experimental conditions making direct comparison between membranes challenging. Here, we develop an in vitro system for evaluating various membranes.</p><p><strong>Methods: </strong>We performed toxin transport studies using commercially available hollow fiber membranes (FX1000, Fresenius) as well as newly developed mixed matrix membrane (MMM) hollow fibers, both assembled in a mini dialyzer. Creatinine (Cr), hippuric acid (HA) and indoxyl sulfate (IS) were used as model uremic toxins and their removal by the membranes from human plasma and full human blood under a range of experimental conditions was studied.</p><p><strong>Results: </strong>We mainly implemented dialysate recirculation, which allowed for monitoring of the mass balance of toxins inside the mini dialyzer and for obtaining better understanding about the membrane fouling and concentration polarization phenomena. Dialysance normalized by membrane effective area was used as a key parameter for assessing the performance of the membranes.</p><p><strong>Conclusions: </strong>Our findings are in good agreement to various literature studies for small and larger scale dialyzers suggesting that the proposed in vitro system can be used for comparison of membranes for HD.</p>","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pushing the Limit for Marginal Grafts in the Era of Machine Perfusion: A Tale of HOPE From a Leading Italian Institution.","authors":"Gerti Dajti, Giuliana Germinario, Enrico Prosperi, Guido Fallani, Chiara Bonatti, Edoardo Prosperi, Antonio Siniscalchi, Maria Cristina Morelli, Antonietta D' Errico, Matteo Serenari, Massimo Del Gaudio, Chiara Zanfi, Federica Odaldi, Lorenzo Maroni, Andrea Laurenzi, Matteo Cescon, Matteo Ravaioli","doi":"10.1111/aor.70137","DOIUrl":"https://doi.org/10.1111/aor.70137","url":null,"abstract":"<p><strong>Background: </strong>The advent of machine perfusion (MP) has significantly improved post-liver transplantation (post-LT) outcomes, potentially enabling the use of increasingly marginal grafts and expanding the organ donor pool.</p><p><strong>Methods: </strong>We present a retrospective cohort study of consecutive adult patients who underwent LT between 2018 and 2023 at a leading Italian institution. The objective was to evaluate outcomes following the use of hypothermic oxygenated perfusion (HOPE) in high-risk grafts.</p><p><strong>Results: </strong>A total of 507 patients were included in the final analysis, of whom 420 (83%) received extended criteria donor (ECD) grafts. Among ECD grafts, 62 (15%) were from donation after circulatory death (DCD) donors, and 64 (15%) were previously discarded by other centers. HOPE was applied in 248 (49%) cases. Recipients in the HOPE group experienced significantly lower rates of early allograft dysfunction (EAD) (20% vs. 32%, p = 0.007), primary nonfunction (2% vs. 7%, p = 0.017), and severe postoperative complications (Clavien-Dindo grade ≥ 3b) (19% vs. 28%, p = 0.026). Notably, marginal grafts treated with HOPE achieved survival outcomes comparable to those of standard risk.</p><p><strong>Conclusions: </strong>HOPE is associated with improved outcomes in LT using ECD grafts and can enable the safe use of higher-risk organs with acceptable results when performed in experienced centers.</p>","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility Testing of the 2nd Generation Inspired Therapeutics NeoMate System for Paracorporeal Support of Neonates and Infants in Heart Failure.","authors":"Gretel Monreal, Steven C Koenig, Mark S Kelley, Joshua G Crane, Isabelle R Lytle, Jiapeng Huang, Mark S Slaughter, Daniel G White, Daniel Tamez, James F Kelley","doi":"10.1111/aor.70155","DOIUrl":"https://doi.org/10.1111/aor.70155","url":null,"abstract":"<p><strong>Background: </strong>Inspired Therapeutics is advancing the development of the next generation of the NeoMate mechanical circulatory support (MCS) system for neonate and infant heart failure (HF) populations. The NeoMate system includes a low-cost magnetically-actuated paracorporeal centrifugal pump with an integrated, reusable motor and controller. This design iteration features a compact size (37 mm diameter), small prime volume (10 mL), and redesigned rotor and flow path. We present experimental data from computational fluid dynamics (CFD), mock loops, and acute animals.</p><p><strong>Methods: </strong>CFD models were used to improve pump design (wall shear stress, hemolysis index) and motor performance (torque, power, efficiency). Static mock loops (3.5 cP) were performed to evaluate hydrodynamics over a range of loads (0-300 mmHg) at 500-5500 rpm (n = 2 pumps tested, 24Fr inflow and 16Fr outflow cannula). A dynamic mock loop (3.5 cP) was tuned to HF, with the pump integrated LV apex-to-aorta and data collected from 0 to 5500 rpm. Two acute sheep studies were performed with each pump implanted paracorporeally: one LV apex-to-aorta (24Fr inflow, 16Fr outflow) and the other via the jugular vein (28Fr dual-lumen cannula).</p><p><strong>Results: </strong>CFD pump and motor performance and hemolysis index metrics were achieved exceeding design criteria (2.5 L/min, 150 mmHg) in static (2.5 L/min flow, 250 mmHg pressure), dynamic (2.8 L/min flow), and animals (3.1 L/min flow, 60 mmHg aortic pressure, plasma free hemoglobin < 20 mg/dL) at 5500 rpm.</p><p><strong>Conclusions: </strong>The next generation NeoMate pumps demonstrated favorable hydrodynamic, hemodynamic, and blood trauma performance, showing promising progress toward this technology to serve as a versatile therapy for neonates and infants.</p>","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Noninvasive Estimation of Left Ventricular End-Diastolic Pressure Through a Mechanical Seal for a Rotary Blood Pump.","authors":"Daisuke Ogawa, Tadashi Motomura, Yasuyuki Shiraishi, Shinji Kobayashi, Jasmine Martinez, Francis C Chikweto, Tomoyuki Yambe, Kenji Yamazaki","doi":"10.1111/aor.70158","DOIUrl":"https://doi.org/10.1111/aor.70158","url":null,"abstract":"<p><strong>Background: </strong>Left ventricular end-diastolic pressure (LVEDP) is an important index of cardiac preload and heart failure (HF) severity. However, direct measurement of LVEDP requires invasive procedures, including local anesthesia and catheter insertion. To address these limitations, we developed a novel LVEDP estimation method based on the relationship between left ventricular pressure and the behavior of the mechanical seal (MS). In this study, we demonstrate that LVEDP can be estimated by measuring pressure inside the MS.</p><p><strong>Methods: </strong>Rotating parts of the RBP, consisting of an impeller, a seal ring, and other components, are supported by a seat ring, while the seal ring is nearly in contact with the seat ring. The clearance width between the rings is determined by the balance of forces acting on the rotating parts, and MS opening occurs when this balance is changed. First, a static test was conducted to evaluate the relationship between MS opening pressure (MSOP) and RBP inlet pressure. Subsequently, a dynamic test using a pulsatile simulator was performed to confirm whether this relationship remains valid under physiological conditions.</p><p><strong>Results: </strong>The static test demonstrated that MSOP is proportional to the RBP inlet pressure. In the dynamic test, MS openings were observed only during diastole, and MSOP showed a strong correlation with LVEDP.</p><p><strong>Conclusion: </strong>Our method successfully estimated LVEDP in a completely noninvasive manner by utilizing the mechanical seal structure of the RBP.</p>","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic Resonance Imaging Evaluation of Functional Differences Between In Vivo Human Kidneys and Discarded Human Kidneys During Ex Vivo Normothermic Machine Perfusion.","authors":"Tim L Hamelink, Chris L Jaynes, L Annick van Furth, Johannes Castelein, Nathan Ooms, Veerle A Lantinga, Baran Ogurlu, Xiaopeng Zhou, Ulrike Dydak, Henri G D Leuvenink, Anna Krarup Keller, Ronald J H Borra, Cyril Moers","doi":"10.1111/aor.70142","DOIUrl":"https://doi.org/10.1111/aor.70142","url":null,"abstract":"<p><strong>Background: </strong>With increasing interest in renal normothermic machine perfusion (NMP), a deeper understanding of ex vivo renal physiology is essential to establish NMP as a robust pre-transplant assessment platform. This study utilized magnetic resonance imaging (MRI) to compare in vivo renal physiology in healthy volunteers with ex vivo renal function of human donor kidneys during NMP.</p><p><strong>Methods: </strong>Multiparametric MRI maps assessing water diffusion, oxygenation, tissue characteristics, and perfusion were obtained from 11 healthy volunteers to define an in vivo renal reference frame. For ex vivo evaluation, 25 discarded human donor kidneys underwent 4 h of oxygenated hypothermic machine perfusion, followed by 6 h of NMP in an MRI-compatible setup. The kidneys were assessed hourly using comparable MRI modalities as in vivo.</p><p><strong>Results: </strong>Ex vivo renal physiology was substantially different from in vivo physiology in terms of MRI-based diffusion patterns, oxygenation, tissue characteristics, and tissue perfusion. Most MRI measurements did not correlate well with conventional parameters such as flow, renal function, and injury markers during NMP.</p><p><strong>Conclusions: </strong>Our findings highlight distinct differences between in vivo and ex vivo MRI-based renal characteristics in a human cohort, suggesting that parameters beyond conventional in vivo functional markers may warrant consideration when evaluating organ viability during NMP.</p>","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Initiation of Neurohormonal Blockade and Clinical Outcomes in HeartMate 3 LVAD Recipients During Index Implantation.","authors":"Flavia Tejada Frisancho, Richa Gupta, Zhi Li, Liza Shaban, Ajay Kadakkal, Miguel Pinilla-Vera, Michael Hockstein, Ezequiel J Molina, Keki Balsara, Farooq H Sheikh, Phillip H Lam","doi":"10.1111/aor.70140","DOIUrl":"https://doi.org/10.1111/aor.70140","url":null,"abstract":"<p><strong>Background: </strong>The use of neurohormonal blockade (NB), including beta-blockers, mineralocorticoid receptor antagonists, and renin angiotensin system inhibitors, in patients after left ventricular assist device (LVAD) implantation is associated with improved clinical outcomes. However, its use during and soon after index LVAD implant discharge, a period of potential hemodynamic and laboratory vulnerability, and impact on outcomes in HeartMate 3 (HM3) recipients remain undefined and is the aim of the current study.</p><p><strong>Methods: </strong>A single-center retrospective analysis was performed of patients undergoing HM3 LVAD implantation and discharged from index implant from January 2015 to December 2021. To align with contemporary management, we included sodium-glucose cotransporter 2 inhibitors in the NB cohort. Patients on and not on any NB after index discharge were compared. Cox proportional hazards modeling was performed to investigate the association between the presence of NB with survival and heart failure (HF) readmission.</p><p><strong>Results: </strong>In total, 273 patients underwent HM3 LVAD implantation during the study period. The average implant age was 58 years, 75% were men, and 78% were Black patients. Of the 273 patients, 138 (50%) had a prescription of at least one NB agent after index discharge. Predictors of NB use included a history of stroke, perioperative renal replacement therapy, and index discharge creatinine level. An index discharge prescription for NB was associated with a lower risk of all-cause mortality at a median follow-up of 3.8 years (age-sex-race adjusted HR 0.63; 95% CI, 0.41-0.98; p = 0.042). The association was attenuated with additional adjustment for the predictors (HR 0.68; 95% CI, 0.43-1.08; p = 0.100). A discharge prescription for NB was not associated with HF readmission.</p><p><strong>Conclusion: </strong>While early initiation of NB therapy in HM3 LVAD recipients was associated with a lower risk of mortality, its clinical impact was attenuated after accounting for baseline and perioperative factors. These findings suggest that NB prescription may reflect underlying clinical stability and accordingly, results should be considered hypothesis-generating and warrant prospective evaluation.</p>","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Efficacy of Smart Saliency Detection System for Visual Prosthesis Users: An Experimental Comparison Across Various Visual Prosthesis Implants.","authors":"Nermin Khalifa, Sahar Selim, Walid Al-Atabany","doi":"10.1111/aor.70147","DOIUrl":"https://doi.org/10.1111/aor.70147","url":null,"abstract":"<p><strong>Background: </strong>Individuals with visual prostheses often struggle to locate specific objects due to limited visual input. Current systems process visual information but fail to effectively highlight or prioritize objects based on user needs.</p><p><strong>Methods: </strong>This study investigates a user-centric object highlighting system designed to assist retinal prosthesis users in finding misplaced objects in a controlled experimental setting. The system uses pre-trained multimodal models, allowing users to specify objects through spoken instructions, which are then highlighted in the camera frame. We evaluated the system's performance in a simulated environment with 18 sighted participants acting as virtual patients. We examined how various retinal implant resolutions and stimulation points, from low to high (60-1600 electrodes), impact object recognition.</p><p><strong>Results: </strong>Mixed-effects logistic regression, both frequentist and Bayesian, revealed significant variance in recognition outcomes based on fixed and random effects. High-resolution implants achieved the best and most consistent recognition rates, while lower-resolution implants showed suboptimal recognition. Notably, across all implants, larger objects were recognized more effectively compared to smaller items, indicating that even higher-resolution implants struggle with smaller objects. The characteristics of these objects, particularly size and distinct features, played a crucial role in their recognition performance. This underscores the necessity for effective detection systems tailored to the capabilities of implants, especially those with lower resolution, which lack sufficient detail for independent object recognition.</p><p><strong>Conclusion: </strong>These findings provide valuable insights for enhancing user-centric object highlighting systems and inform the development of real-world testing in complex environments.</p>","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Gentamicin-Impregnated Collagen Sponge on the Incidence of Access Related Infections After Full-Support Microaxial Flow Pump Therapy.","authors":"Gaik Nersesian, Maximilian Rauch, Daniel Lewin, Leonard Pitts, Julius Kaemmel, Anna Stegmann, Julia Stein, Yuriy Hrytsyna, Felix Schoenrath, Volkmar Falk, Evgenij Potapov, Pia Lanmueller","doi":"10.1111/aor.70141","DOIUrl":"https://doi.org/10.1111/aor.70141","url":null,"abstract":"<p><strong>Background: </strong>Surgically implanted microaxial flow pumps (mAFP) are increasingly used for cardiogenic shock treatment. Standard mAFP explantation is performed bedside with a shortening of the vascular prosthesis, which may represent a potential source of infection in future. We analyzed the impact of gentamicin-impregnated collagen sponge (GICS) application in the wound during mAFP explantation on the incidence of prosthetic graft infections.</p><p><strong>Methods: </strong>Between 01/2020 and 07/2024, 235 patients underwent bedside full-support mAFP explantation at our institution. Since 11/2022, GICS has been routinely applied in 115 patients, while 120 previously were treated without it.</p><p><strong>Results: </strong>In the GICS-group, after a median follow-up of 221 [24; 351] days, 17 (14.8%) patients developed graft infection, resulting in 0.23 events per patient year (EPPY); surgery was necessary in 16 patients (13.9%). In the control group, after a median follow-up of 399 [113; 654] days, infection occurred in 14 (11.7%) cases, 0.10 EPPY; surgical removal of the graft was necessary in 12 (10%). The GICS-groups presented an increased risk for graft infections: sHR 2.49 [1.11; 5.59], p = 0.027. After propensity score matching for relevant demographic parameters, there was no significant difference in the risk reduction of total graft infections sHR 1.92 [0.79; 4,72], p = 0.15.</p><p><strong>Conclusions: </strong>Local application of GICS did not reduce the risk of access site infections in patients undergoing bedside explantation of mAFP from the graft surgically anastomosed to an axillary artery.</p>","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Autologous Tissue Sources and Optimization of a Treatment Method for Intraoperative Manufacturing of Heart Valve Constructs.","authors":"Marvin Steitz, Mahamuda Badhon Khan, Katharina Fritsch, Alexander Breitenstein-Attach, Jana Katharina Betty Korbacher, Jordi Joaquin Modolell Y Mainou, Katrin Schrimpf, Robert Ramm, Sara Knigge, Katja Eildermann, Georg Arlt, Antonia Schulz, Jonathan Frederik Sebastian Kiekenap, Boris Warnack, Peter Kramer, Frank Edelmann, Felix Berger, Boris Schmitt","doi":"10.1111/aor.70151","DOIUrl":"https://doi.org/10.1111/aor.70151","url":null,"abstract":"<p><strong>Introduction: </strong>Valvular heart disease remains a leading cause of morbidity and mortality. Current biological and mechanical heart valve prostheses have significant limitations and fail to address the patient's needs. Most of the limitations arise from the use of foreign materials, which can largely be addressed by utilizing autologous pericardial tissue. However, not all patients have usable pericardium due to previous interventions. This study investigates peritoneum as a potential alternative to pericardium and evaluates an optimized treatment method for intraoperative tissue treatment.</p><p><strong>Methods: </strong>A previously established cross-linking method was optimized and applied to pericardial and peritoneal tissues. The tissues were characterized with respect to biochemical composition, cross-linking degree, biomechanical properties, and structural organization. Furthermore, the feasibility of valve shaping was assessed, and constructs were subjected to acute hydrodynamic testing.</p><p><strong>Results: </strong>Tissue treated with the optimized cross-linking method showed high cytocompatibility and was successfully applied to peritoneal tissue. Compared to pericardium, peritoneum exhibited a higher elastin content and a looser structural organization, resulting in distinct mechanical behavior. Both tissues could be shaped into valve constructs showing adequate acute hydrodynamic behavior under the applied test conditions.</p><p><strong>Conclusion: </strong>The findings suggest that the peritoneum is a promising candidate material for further investigation as a heart valve scaffold. However, differences in microstructure and mechanical behavior highlight the need for further testing, particularly regarding long-term durability, cyclic loading behavior, and tissue remodeling capacity.</p>","PeriodicalId":8450,"journal":{"name":"Artificial organs","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}