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Artificial cells, blood substitutes, and immobilization biotechnology最新文献

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Synergistic effect of IFN-γ gene on LIGHT-induced apoptosis in HepG2 cells via down regulation of Bcl-2. IFN-γ基因通过下调Bcl-2在光诱导HepG2细胞凋亡中的协同作用
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2011-08-01 Epub Date: 2010-11-30 DOI: 10.3109/10731199.2010.538403
Bing Han, Li-Qun Wu, Xiang Ma, Zheng-Hua Wang, Jin-Peng Li, Chong-Yao Bi, Sun Yong
{"title":"Synergistic effect of IFN-γ gene on LIGHT-induced apoptosis in HepG2 cells via down regulation of Bcl-2.","authors":"Bing Han,&nbsp;Li-Qun Wu,&nbsp;Xiang Ma,&nbsp;Zheng-Hua Wang,&nbsp;Jin-Peng Li,&nbsp;Chong-Yao Bi,&nbsp;Sun Yong","doi":"10.3109/10731199.2010.538403","DOIUrl":"https://doi.org/10.3109/10731199.2010.538403","url":null,"abstract":"<p><p>To detect the expression of anti-apoptotic factor Bcl-2 and Survivin in transferred HepG2 cells and evaluate the synergistic effect of IFN-γ gene on LIGHT-induced apoptosis signal transduction pathways, the full-length ORF of LIGHT and IFN-γ gene were cloned into pcDNA4 and verified by DNA sequencing. After being optimized by EGFP, recombinant LIGHT and IFN-γ were transferred into the HepG2 cells mediated by a cationic liposome in vitro. The expression of LIGHT and IFN-γ was identified in the supernatants by ELISA. The HepG2 cells were divided into three groups: the control, LIGHT gene transfection alone, and simultaneous transfection of LIGHT and IFN-γ genes. The cell apoptosis and expression of Bcl-2 and Survivin in cell lysate were detected through FCM. After transfection, the apoptosis rate of HepG2 cells was increased with the prolonged time, and the apoptosis rate of LIGHT group was higher than the control group, while the LIGHT/IFN-γ group was higher than the LIGHT group P < 0.01). The expression of Bcl-2 and Survivin in LIGHT group and LIGHT/IFN-γ group decreased dramatically compared with the control group. LIGHT gene alone can result in significant inhibition of HepG2 cells proliferation. INF-γ can synergistically precede LIGHT-induced apoptotic processes through down-regulation of Bcl-2 expression, but not survivin expression.</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2010.538403","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29438945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Potential c-myc antisense oligonucleotide carriers: PCl/PEG/PEI and PLL/PEG/PEI. 潜在的c-myc反义寡核苷酸载体:PCl/PEG/PEI和PLL/PEG/PEI。
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2011-06-01 Epub Date: 2010-08-02 DOI: 10.3109/10731199.2010.506852
Sevil Dinçer, Mustafa Türk, Ayşe Karagöz, Gürhan Uzunalan
{"title":"Potential c-myc antisense oligonucleotide carriers: PCl/PEG/PEI and PLL/PEG/PEI.","authors":"Sevil Dinçer,&nbsp;Mustafa Türk,&nbsp;Ayşe Karagöz,&nbsp;Gürhan Uzunalan","doi":"10.3109/10731199.2010.506852","DOIUrl":"https://doi.org/10.3109/10731199.2010.506852","url":null,"abstract":"<p><p>In this work, positively charged, micelle-forming polymers were synthesized and used as a model vector to deliver antisense oligodeoxynucleotide (ASODN) into melanoma cells. Polymers and polymer/ASODN complexes were characterized by DLS according to size, charge, and critical micelle concentration. Nanosize and spherical complexes were observed by AFM. Complexes did not reveal significant toxicity to melanoma cells. Antiproliferative effect of the complexes was observed by immunocytochemical staining and estimated as 56.8% with N/P:9. High amount of apoptosis and very small amount of necrosis were estimated. According to the results, these positively charged polymers forming micelle-like structures seem promising as ASODN carriers.</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2010.506852","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29163257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Enantioselective production of benzoin from benzoin acetate via kinetic resolution and deracemization using Rhizopus oryzae. 米根霉动力学拆分和脱羧对映选择性地从醋酸安息香生产安息香。
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2011-06-01 Epub Date: 2010-09-21 DOI: 10.3109/10731199.2010.516261
Rahime Songür, Binnaz Lurçi, Emine Bayraktar, Ulkü Mehmetoğlu, Ayhan S Demir
{"title":"Enantioselective production of benzoin from benzoin acetate via kinetic resolution and deracemization using Rhizopus oryzae.","authors":"Rahime Songür,&nbsp;Binnaz Lurçi,&nbsp;Emine Bayraktar,&nbsp;Ulkü Mehmetoğlu,&nbsp;Ayhan S Demir","doi":"10.3109/10731199.2010.516261","DOIUrl":"https://doi.org/10.3109/10731199.2010.516261","url":null,"abstract":"<p><p>In this study, the production of enantiopure benzoin from rac-benzoin acetate was achieved by lipase catalyzed kinetic resolution combined with deracemization using Rhizopus oryzae (CBS111718). The growth cells were pretreated with 20 kHz and 30 kHz ultrasound irradiation and mechanical homogenization. Approximately 100% conversion and 96% enantiomeric excess of the product (S-benzoin) were obtained by applying 20 kHz ultrasound irradiation at pH 6. The deracemization process involves new and important processes that allow for the transformation of a racemate into a single stereoisomeric product in 100% theoretical yields. Moreover, the application of ultrasound increases the conversion rate by reducing mass transfer limitation.</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2010.516261","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40083379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Enhanced stability and decolorization of Coomassie Brilliant Blue R-250 by dextran aldehyde-modified horseradish peroxidase. 葡聚糖醛修饰的辣根过氧化物酶提高考马斯亮蓝R-250的稳定性和脱色效果。
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2011-06-01 Epub Date: 2010-11-30 DOI: 10.3109/10731199.2010.533124
Melda Altikatoglu, Mithat Celebi
{"title":"Enhanced stability and decolorization of Coomassie Brilliant Blue R-250 by dextran aldehyde-modified horseradish peroxidase.","authors":"Melda Altikatoglu,&nbsp;Mithat Celebi","doi":"10.3109/10731199.2010.533124","DOIUrl":"https://doi.org/10.3109/10731199.2010.533124","url":null,"abstract":"<p><p>Horseradish peroxidase (EC 1.11.1.7) was chemically modified by periodate-activated dextran. The activities of free and modified enzyme against organic-aqueous interface and some chemicals were determined. Modified HRP remained fully active in the presence of organic solvent for 4 h. However, the unmodified enzyme lost 50% of its activity within the first 2 h. The effects of possible inhibitors on enzyme activity were investigated. In addition, Coomassie Brilliant Blue R-250 was efficiently decolorized using the free and modified HRP. After 5 minutes of treatment, the color removal of dye was 80-90%. Modified HRP showed effective performance compared to free HRP.</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2010.533124","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29438948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Preparation and the influencing factors of cetirizine hydrochloride microemulsion. 盐酸西替利嗪微乳的制备及其影响因素。
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2011-06-01 Epub Date: 2010-10-15 DOI: 10.3109/10731199.2010.525710
Yong Sun, Yue Jiang, Keyao An
{"title":"Preparation and the influencing factors of cetirizine hydrochloride microemulsion.","authors":"Yong Sun,&nbsp;Yue Jiang,&nbsp;Keyao An","doi":"10.3109/10731199.2010.525710","DOIUrl":"https://doi.org/10.3109/10731199.2010.525710","url":null,"abstract":"<p><p>We constructed pseudo-ternary phase diagrams to obtain the area of water-in-oil (w/o) microemulsion formation, prepared cetirizine hydrochloride microemulsion, and evaluated its quality. The w/o microemulsion had been formulated using span80 as surfactant, alcohol as co-surfactant, and oleinic acid as oil. Pseudo-ternary phase diagrams were constructed to evaluate the phase behavior of the w/o microemulsion. The properties, including appearance, particle size, and stability, were determined. The stable w/o microemulsion could be prepared using span80/ alcohol/ oleinic acid/ water (1.2/ 4.8/ 2/ 2). The w/o microemulsion is a new carrier for hydrosoluble drugs, which are easy to prepare with consistent quality.</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2010.525710","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29354876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Pharmacodynamic study of polymerized porcine hemoglobin (pPolyHb) in a rat model of exchange transfusion. 聚合猪血红蛋白(polyhb)在大鼠换血模型中的药效学研究。
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2011-06-01 Epub Date: 2011-03-07 DOI: 10.3109/10731199.2011.559584
Hongli Zhu, Xiaodong Dang, Kunping Yan, Penggao Dai, Chao Luo, Jun Ma, Yan Li, Thomas Ming Swi Chang, Chao Chen
{"title":"Pharmacodynamic study of polymerized porcine hemoglobin (pPolyHb) in a rat model of exchange transfusion.","authors":"Hongli Zhu,&nbsp;Xiaodong Dang,&nbsp;Kunping Yan,&nbsp;Penggao Dai,&nbsp;Chao Luo,&nbsp;Jun Ma,&nbsp;Yan Li,&nbsp;Thomas Ming Swi Chang,&nbsp;Chao Chen","doi":"10.3109/10731199.2011.559584","DOIUrl":"https://doi.org/10.3109/10731199.2011.559584","url":null,"abstract":"<p><p>The objective of the present study is to evaluate the pharmacodynamic properties of polymerized porcine hemoglobin (pPolyHb) in an exchange transfusion model. Each of two groups of rats received a volume of pPolyHb or hetastarch that equalled 120-140% of estimated total blood volume (70 ml/kg) exchange transfusion. The results showed pPolyHb retained hemodynamic stability and exhibited superior volume expansion capability. Furthermore, pPolyHb effectively reverse anaerobic metabolism caused by a large amount of volume exchange. In comparison with hetastarch, pPolyHb increased blood oxygen content and tissue oxygenation. All these properties contribute to a higher effectiveness in sustaining the lives of rats in pPolyHb group.</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2011.559584","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29722415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase: a novel biotechnology-based blood substitute that transports both oxygen and carbon dioxide and also acts as an antioxidant. 多血红蛋白-超氧化物歧化酶-过氧化氢酶-碳酸酐酶:一种新型的基于生物技术的血液替代品，可运输氧气和二氧化碳，也可作为抗氧化剂。
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2011-06-01 DOI: 10.3109/10731199.2011.581052
Yuzhu Bian, Zhixia Rong, Thomas Ming Swi Chang
{"title":"Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase: a novel biotechnology-based blood substitute that transports both oxygen and carbon dioxide and also acts as an antioxidant.","authors":"Yuzhu Bian,&nbsp;Zhixia Rong,&nbsp;Thomas Ming Swi Chang","doi":"10.3109/10731199.2011.581052","DOIUrl":"https://doi.org/10.3109/10731199.2011.581052","url":null,"abstract":"<p><p>Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase (PolyHb-SOD-CAT-CA) is a therapeutic antioxidant that also transports both oxygen and carbon dioxide. This is formed by crosslinking Hb with SOD, CAT, and CA using glutaraldehyde. Crosslinking stroma free Hb from red blood cell (rbc) reduces CA activity to 55%. Addition of more CA resulted in a preparation with the same CA activity as RBC. PolyHb in the complex acts as a buffer to prevent large pH changes as carbon dioxide is converted to carbonic acid. We then prepare and optimize a novel PolyHb-SOD-CAT-CA, a therapeutic antioxidant that also transports both oxygen and carbon dioxide.</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2011.581052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30189698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 46
Letter to the editor: What's in a name? 给编辑的信:名字有什么意义?
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2011-06-01 Epub Date: 2010-07-19 DOI: 10.3109/10731199.2010.502881
Timothy N Estep
{"title":"Letter to the editor: What's in a name?","authors":"Timothy N Estep","doi":"10.3109/10731199.2010.502881","DOIUrl":"https://doi.org/10.3109/10731199.2010.502881","url":null,"abstract":"community that has led to a sometimes destructive polarization between those of us engaged in the development of alternative oxygen transport formulations and those working on a daily basis to implement and improve the current practice of transfusion. The reality is that even if alternative oxygen carriers meet our wildest expectations, there will be a need for traditional blood product collection, distribution, and administration for the foreseeable future. On the other hand, having alternatives with different risk/benefi t profi les than red cells, as well as logistical and/or immunologic advantages, will undoubtedly improve the practice of medicine. Having only one modality to treat the enormous range of hypoxic and ischemic challenges facing patients is analogous to having only one antibiotic to treat all infections, or one chemotherapeutic to treat all cancers. Rather than defensive argumentation, a more constructive approach would be the establishment of a forthright and constructive dialogue about the limitations and advantages of both current blood transfusion practices and alternative oxygen carriers to establish the most effi cient and co-operative way forward to the improvement of patient care. This will require a number of outreach steps between those in the traditional blood transfusion community and those attempting to develop alternatives. One small, but not insignifi cant, step in this direction would be replacement of the label “ blood substitutes ” with a more accurate and less provocative terminology.","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2010.502881","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29125599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Construction of an amperometric ascorbate biosensor using epoxy resin membrane bound Lagenaria siceraria fruit ascorbate oxidase. 利用环氧树脂膜结合黑根草果实抗坏血酸氧化酶构建安培型抗坏血酸生物传感器。
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2011-06-01 Epub Date: 2010-11-10 DOI: 10.3109/10731199.2010.525711
C S Pundir, Nidhi Chauhan, Jyoti
{"title":"Construction of an amperometric ascorbate biosensor using epoxy resin membrane bound Lagenaria siceraria fruit ascorbate oxidase.","authors":"C S Pundir,&nbsp;Nidhi Chauhan,&nbsp;Jyoti","doi":"10.3109/10731199.2010.525711","DOIUrl":"https://doi.org/10.3109/10731199.2010.525711","url":null,"abstract":"<p><p>Ascorbate oxidase purified from Lagenaria siceraria fruit was immobilized onto epoxy resin \"Araldite\" membrane with 79.4% retention of initial activity of free enzyme. The biosensor showed optimum response within 15s at pH 5.8 and 35°C, which was directly proportional to ascorbate concentration ranging from 1-100μM. There was a good correlation (R(2) = 0.99) between serum ascorbic acid values by standard enzymic colorimetric method and the present method. The enzyme electrode was used for 200 times without considerable loss of activity during the span of 90 days when stored at 4°C.</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2010.525711","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29460525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Pulsed laser light forces cancer cells to absorb anticancer drugs--the role of water in nanomedicine. 脉冲激光迫使癌细胞吸收抗癌药物——就像水在纳米医学中的作用。
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2011-06-01 Epub Date: 2010-09-17 DOI: 10.3109/10731199.2010.516262
Andrei P Sommer, Dan Zhu, Adam R Mester, Horst-Dieter Försterling
{"title":"Pulsed laser light forces cancer cells to absorb anticancer drugs--the role of water in nanomedicine.","authors":"Andrei P Sommer,&nbsp;Dan Zhu,&nbsp;Adam R Mester,&nbsp;Horst-Dieter Försterling","doi":"10.3109/10731199.2010.516262","DOIUrl":"https://doi.org/10.3109/10731199.2010.516262","url":null,"abstract":"Abstract: Anticancer drugs executing their function intracellularly enter cancer cells via diffusive processes. Complementary to these slow processes, cells can be forced to incorporate drugs by convection – a more efficient transport process. Transmembrane convection is induced by moderately intense pulsed laser light (or light emitting diodes) changing the structure of nanoscopic water layers in cells. This is a fundamental difference with the method of photodynamic therapy. In a model system we demonstrate that a total irradiation time of one minute is sufficient to completely inhibit proliferation of cancer cells. Transmembrane convection protects healthy cells from extended chemotherapy exposure, could be exploited to overcome multidrug resistance, and is a promising new tool in a variety of therapies as well as in skin rejuvenation.","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2010.516262","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40075939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
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