葡聚糖醛修饰的辣根过氧化物酶提高考马斯亮蓝R-250的稳定性和脱色效果。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2011-06-01 Epub Date: 2010-11-30 DOI:10.3109/10731199.2010.533124

Melda Altikatoglu, Mithat Celebi

{"title":"葡聚糖醛修饰的辣根过氧化物酶提高考马斯亮蓝R-250的稳定性和脱色效果。","authors":"Melda Altikatoglu, Mithat Celebi","doi":"10.3109/10731199.2010.533124","DOIUrl":null,"url":null,"abstract":"Horseradish peroxidase (EC 1.11.1.7) was chemically modified by periodate-activated dextran. The activities of free and modified enzyme against organic-aqueous interface and some chemicals were determined. Modified HRP remained fully active in the presence of organic solvent for 4 h. However, the unmodified enzyme lost 50% of its activity within the first 2 h. The effects of possible inhibitors on enzyme activity were investigated. In addition, Coomassie Brilliant Blue R-250 was efficiently decolorized using the free and modified HRP. After 5 minutes of treatment, the color removal of dye was 80-90%. Modified HRP showed effective performance compared to free HRP.","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":"39 3","pages":"185-90"},"PeriodicalIF":0.0000,"publicationDate":"2011-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2010.533124","citationCount":"17","resultStr":"{\"title\":\"Enhanced stability and decolorization of Coomassie Brilliant Blue R-250 by dextran aldehyde-modified horseradish peroxidase.\",\"authors\":\"Melda Altikatoglu, Mithat Celebi\",\"doi\":\"10.3109/10731199.2010.533124\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Horseradish peroxidase (EC 1.11.1.7) was chemically modified by periodate-activated dextran. The activities of free and modified enzyme against organic-aqueous interface and some chemicals were determined. Modified HRP remained fully active in the presence of organic solvent for 4 h. However, the unmodified enzyme lost 50% of its activity within the first 2 h. The effects of possible inhibitors on enzyme activity were investigated. In addition, Coomassie Brilliant Blue R-250 was efficiently decolorized using the free and modified HRP. After 5 minutes of treatment, the color removal of dye was 80-90%. Modified HRP showed effective performance compared to free HRP.\",\"PeriodicalId\":8413,\"journal\":{\"name\":\"Artificial cells, blood substitutes, and immobilization biotechnology\",\"volume\":\"39 3\",\"pages\":\"185-90\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3109/10731199.2010.533124\",\"citationCount\":\"17\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Artificial cells, blood substitutes, and immobilization biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3109/10731199.2010.533124\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2010/11/30 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Artificial cells, blood substitutes, and immobilization biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/10731199.2010.533124","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2010/11/30 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 17

摘要

用高碘酸活化的葡聚糖对辣根过氧化物酶(EC 1.11.1.7)进行化学修饰。测定了游离酶和修饰酶对有机-水界面和某些化学物质的活性。改性后的HRP在有机溶剂中保持充分活性4小时。然而，未改性的酶在前2小时内失去50%的活性。研究了可能的抑制剂对酶活性的影响。此外，comasassie Brilliant Blue R-250使用自由和改性HRP有效脱色。处理5分钟后，染料脱色率为80-90%。与游离HRP相比，改性HRP表现出了更好的性能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Enhanced stability and decolorization of Coomassie Brilliant Blue R-250 by dextran aldehyde-modified horseradish peroxidase.

Horseradish peroxidase (EC 1.11.1.7) was chemically modified by periodate-activated dextran. The activities of free and modified enzyme against organic-aqueous interface and some chemicals were determined. Modified HRP remained fully active in the presence of organic solvent for 4 h. However, the unmodified enzyme lost 50% of its activity within the first 2 h. The effects of possible inhibitors on enzyme activity were investigated. In addition, Coomassie Brilliant Blue R-250 was efficiently decolorized using the free and modified HRP. After 5 minutes of treatment, the color removal of dye was 80-90%. Modified HRP showed effective performance compared to free HRP.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Artificial cells, blood substitutes, and immobilization biotechnology 工程技术-材料科学：生物材料

自引率

0.00%

发文量

审稿时长

6-12 weeks