多血红蛋白-超氧化物歧化酶-过氧化氢酶-碳酸酐酶:一种新型的基于生物技术的血液替代品，可运输氧气和二氧化碳，也可作为抗氧化剂。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2011-06-01 DOI:10.3109/10731199.2011.581052

Yuzhu Bian, Zhixia Rong, Thomas Ming Swi Chang

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引用次数: 46

摘要

多血红蛋白-超氧化物歧化酶-过氧化氢酶-碳酸酐酶(PolyHb-SOD-CAT-CA)是一种治疗性抗氧化剂，也运输氧气和二氧化碳。这是通过使用戊二醛将Hb与SOD、CAT和CA交联而形成的。从红细胞(rbc)中交联无基质的Hb可将CA活性降低至55%。添加更多的CA导致制剂具有与RBC相同的CA活性。当二氧化碳转化为碳酸时，复合物中的多聚血红蛋白起到缓冲作用，防止pH值发生大的变化。然后，我们制备并优化了一种新型的PolyHb-SOD-CAT-CA，一种治疗性抗氧化剂，也可以运输氧气和二氧化碳。

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Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase: a novel biotechnology-based blood substitute that transports both oxygen and carbon dioxide and also acts as an antioxidant.

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Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase: a novel biotechnology-based blood substitute that transports both oxygen and carbon dioxide and also acts as an antioxidant.

Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase (PolyHb-SOD-CAT-CA) is a therapeutic antioxidant that also transports both oxygen and carbon dioxide. This is formed by crosslinking Hb with SOD, CAT, and CA using glutaraldehyde. Crosslinking stroma free Hb from red blood cell (rbc) reduces CA activity to 55%. Addition of more CA resulted in a preparation with the same CA activity as RBC. PolyHb in the complex acts as a buffer to prevent large pH changes as carbon dioxide is converted to carbonic acid. We then prepare and optimize a novel PolyHb-SOD-CAT-CA, a therapeutic antioxidant that also transports both oxygen and carbon dioxide.

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来源期刊

Artificial cells, blood substitutes, and immobilization biotechnology 工程技术-材料科学：生物材料

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6-12 weeks