Archives of Physiology and Biochemistry最新文献_第5页

Protein cotargeting of mechanotransduction pathways improves functional orthopedic repair while enhancing anesthetic physiological stability. 机械转导途径的蛋白共靶向改善功能性骨科修复，同时增强麻醉生理稳定性。

IF 2.7 4区医学

Archives of Physiology and Biochemistry Pub Date : 2026-06-11 DOI: 10.1080/13813455.2026.2675976

Weichang Luo, Yajun Zhou, Meixin Wang

{"title":"Protein cotargeting of mechanotransduction pathways improves functional orthopedic repair while enhancing anesthetic physiological stability.","authors":"Weichang Luo, Yajun Zhou, Meixin Wang","doi":"10.1080/13813455.2026.2675976","DOIUrl":"https://doi.org/10.1080/13813455.2026.2675976","url":null,"abstract":"Introduction: Orthopaedic repair outcomes are limited by disrupted cellular mechanotransduction and physiological instability during anaesthesia. Integrating biomechanical signal modulation with stabilising perioperative strategies may enhance tissue healing and reduce postsurgical complications. Methodology: Existing orthopaedic therapies fail to restore native mechanosensory signalling at repair sites, leading to delayed recovery, implant loosening, and reduced function. Additionally, anaesthesia-induced cardiovascular and metabolic changes disrupt regenerative signalling. This paper presents GEM-TRAP, a synergistic cotargeting platform using dual delivery of gene vectors to enhance mechanotransduction and protein regulators to maintain physiological stability during anaesthesia. Smart biomaterial scaffolds enable controlled release at the injury site. Results: In vivo results show improved osseointegration, a 38% increase in load-bearing strength, enhanced neural-mechanical signalling, and a 27% reduction in complications, demonstrating GEM-TRAP's potential for advanced orthopaedic regeneration. The proposed method achieves the osseointegration efficiency of 90%, physiological stability of 92%, mechanical strength recovery of 90%, mitochondrial efficiency of 90%, and postsurgical complications reduced by 40-60%. Conclusion: This study presents GEM-TRAP, a novel cotargeting platform that concurrently enhances cellular mechanotransduction and maintains physiological stability during anaesthesia. By integrating dual therapeutic delivery with smart biomaterial scaffolds, the approach addresses both regenerative and perioperative challenges, offering a new strategy for improving orthopaedic repair and functional recovery.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-16"},"PeriodicalIF":2.7,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148216152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of AGK-2 treatment on sirtuin-2, oxidative stress and apoptosis in ageing pancreatic tissue of rats. AGK-2对衰老大鼠胰腺组织sirtuin-2、氧化应激及细胞凋亡的影响。

IF 3.2 4区医学

Archives of Physiology and Biochemistry Pub Date : 2026-06-01 Epub Date: 2026-04-17 DOI: 10.1080/13813455.2026.2657419

Saide Bakal Muratoğlu, Arzu Keskin Aktan, Cansu Babahan, K Gonca Akbulut

{"title":"Effects of AGK-2 treatment on sirtuin-2, oxidative stress and apoptosis in ageing pancreatic tissue of rats.","authors":"Saide Bakal Muratoğlu, Arzu Keskin Aktan, Cansu Babahan, K Gonca Akbulut","doi":"10.1080/13813455.2026.2657419","DOIUrl":"10.1080/13813455.2026.2657419","url":null,"abstract":"Context: Sirtuin-2 (SIRT2) is a longevity-related protein implicated in apoptosis, metabolic regulation and oxidative stress; however, its role in ageing pancreas remains unclear. Objective: This study investigated the effects of the selective SIRT2 inhibitor, Arabidopsis guanylate kinases-2 (AGK-2) on oxidative stress and apoptosis in aged rat pancreatic tissue. Methods: Young (3-month) and aged (22-month) Wistar albino rats were divided into control and AGK-2-treated groups (10 μM, 30 days). Total oxidant status (TOS) and total antioxidant status (TAS) used commercial kits, then calculated Oxidative stress index (OSI). For detecting nitrosative stress, we determined nitrite-nitrate levels using the Griess test. SIRT2 and caspase-3 were evaluated by ELISA and Western blot. Discussion and conclussion: Our findings showed that ageing increased SIRT2 expression, oxidative stress and caspase-3 protein compared to young rats. AGK-2 treatment reduced SIRT2, TOS, OSI and caspase-3, and increased TAS particularly in aged rats. AGK-2 reverses ageing-associated changes in pancreatic oxidative stress and apoptosis. SIRT2 modulation may represent a potential therapeutic target against age-related pancreatic degeneration.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"349-355"},"PeriodicalIF":3.2,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147715724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fructooligosaccharide supplementation alleviates obesity-related kidney injury in a high-fat diet rat model. 低聚果糖可减轻高脂饮食大鼠模型中肥胖相关性肾损伤。

IF 2.7 4区医学

Archives of Physiology and Biochemistry Pub Date : 2026-06-01 Epub Date: 2025-11-03 DOI: 10.1080/13813455.2025.2581993

Keerati Wanchai, Atcharaporn Ontawong, Keerakarn Somsuan, Arunothai Wanta, Benjamard Sukjai, Siwaporn Praman

引用次数: 0

Possible physiological benefits of fasting and exercise. 禁食和运动可能带来的生理益处。

IF 2.7 4区医学

Archives of Physiology and Biochemistry Pub Date : 2026-06-01 Epub Date: 2025-08-26 DOI: 10.1080/13813455.2025.2550470

Hamed Alizadeh Pahlavani, Ali Veisi

引用次数: 0

The inhibitory impact of glutathione (GSH) and ascorbic acid (vitamin C) compounds on glucose-6-phosphate dehydrogenase (G6PD) enzyme purified from sheep liver. 谷胱甘肽（GSH）和抗坏血酸（维生素C）化合物对绵羊肝脏中纯化的葡萄糖-6-磷酸脱氢酶（G6PD）酶的抑制作用。

IF 2.7 4区医学

Archives of Physiology and Biochemistry Pub Date : 2026-06-01 Epub Date: 2025-10-07 DOI: 10.1080/13813455.2025.2567343

Zehra Bas, Vedat Turkoglu

{"title":"The inhibitory impact of glutathione (GSH) and ascorbic acid (vitamin C) compounds on glucose-6-phosphate dehydrogenase (G6PD) enzyme purified from sheep liver.","authors":"Zehra Bas, Vedat Turkoglu","doi":"10.1080/13813455.2025.2567343","DOIUrl":"10.1080/13813455.2025.2567343","url":null,"abstract":"Objective: Glucose-6-phosphate dehydrogenase (G6PD) is an enzyme with many essential biochemical functions. However, in various cancer diseases, increased activity of G6PD causes cancer cells to grow, so G6PD inhibitors have become a significant area of research in cancer treatment.Materials and methods: Here, G6PD was purified 4530-fold with affinity chromatography using 2',5'-ADP Sepharose 4B from sheep liver. The effects of reduced glutathione (GSH) and ascorbic acid (vitamin C) on G6PD activity were explored.Results and discussion: GSH and ascorbic acid showed a significant inhibitory effect on G6PD, and IC50 values were found as 0.37 µM and 34.66 µM, respectively. The inhibition type from Lineweaver-Burk plots of these compounds was identified as non-competitive inhibition. The Ki values of GSH and ascorbic acid were calculated as 0.48 µM and 30.47 µM, respectively.Conclusion: In this study, it was observed that GSH and ascorbic acid antioxidant compounds exhibit an inhibitory effect on G6PD and may be protective and preventive against cancer.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"278-287"},"PeriodicalIF":2.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of lipid peroxidation and chronic inflammation in development of post-thrombotic syndrome. 脂质过氧化和慢性炎症在血栓形成后综合征发展中的作用。

IF 2.7 4区医学

Archives of Physiology and Biochemistry Pub Date : 2026-06-01 Epub Date: 2025-12-05 DOI: 10.1080/13813455.2025.2592017

Hanjun Wang, Jinhua Jiang

{"title":"Role of lipid peroxidation and chronic inflammation in development of post-thrombotic syndrome.","authors":"Hanjun Wang, Jinhua Jiang","doi":"10.1080/13813455.2025.2592017","DOIUrl":"10.1080/13813455.2025.2592017","url":null,"abstract":"Post-thrombotic syndrome (PTS), a chronic complication of deep vein thrombosis, causes pain, swelling, and ulcers, impairing quality of life. This study explores the role of lipid peroxidation and chronic inflammation in PTS. Oxidative stress and chronic inflammation damage the endothelium, increase venous pressure, and induce fibrosis, driving PTS progression. A biomarker-based ELISA approach is proposed to measure plasma lipid peroxidation products (e.g. MDA) and pro-inflammatory cytokines (e.g. IL-6, TNF-α). This method detects early biochemical changes in post-DVT patients and tracks PTS risk. Elevated oxidative and inflammatory markers are strongly linked to PTS, supporting their use as diagnostic and therapeutic targets. Screening may improve prediction (98.7%), prevention (97.3%), and management (99.4%), while reducing MDA (95.6%), IL-6 (93.4%), and TNF-α (97.45%). Observational NHS data support symptom-biomarker correlations. Preliminary values for MDA (14.3 ± 4.1 nmol/mL), IL-6 (2.3 ± 1.1 pg/mL), and TNF-α (1.5 ± 0.7 pg/mL) are included to clearly present and support the research hypothesis.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"330-348"},"PeriodicalIF":2.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145676401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective role of vildagliptin against bisphenol-A induced liver injury: targeting oxidative stress, apoptosis, and endoplasmic reticulum stress. 维格列汀对双酚a诱导的肝损伤的保护作用：针对氧化应激、细胞凋亡和内质网应激。

IF 2.7 4区医学

Archives of Physiology and Biochemistry Pub Date : 2026-06-01 Epub Date: 2025-09-04 DOI: 10.1080/13813455.2025.2555473

Naglaa Adly Abd Elazeem, Lamis Abdelghani Salamah, Marwa Abdeltawab Mohammed, Ghada Mahmoud Abd El Aziz, Shaimaa Abd El Tawab Fathi

{"title":"Protective role of vildagliptin against bisphenol-A induced liver injury: targeting oxidative stress, apoptosis, and endoplasmic reticulum stress.","authors":"Naglaa Adly Abd Elazeem, Lamis Abdelghani Salamah, Marwa Abdeltawab Mohammed, Ghada Mahmoud Abd El Aziz, Shaimaa Abd El Tawab Fathi","doi":"10.1080/13813455.2025.2555473","DOIUrl":"10.1080/13813455.2025.2555473","url":null,"abstract":"Bisphenol-A (BPA) is an environmental pollutant that causes hepatic injury. The antioxidant activity of vildagliptin is confirmed. The present study investigated the protective effect of Vildagliptin against BPA-induced hepatotoxicity. Twenty four rats were divided randomly into 4 groups (6 rats/group): A control group, BPA group, BPA + Vildagliptin group and Vildagliptin group. All rats, except the controls were orally administered 30 mg/kg body weight BPA and/or 10 mg/kg Vildagliptin. AST, ALT, Triglycerides and albumin were measured in the serum. MDA, GPX, XBP1, Caspase 3 and BCL2 were measured in liver tissues. BPA group showed a significant decrease of albumin and GPX and a significant increase of triglycerides, AST, ALT and MDA. BPA caused up regulation of caspase3 and XBP1 while caused down regulation of BCL2. The co-administration of Vildagliptin reversed these hazards. The results of this study established the protective effect of Vildagliptin against BPA induced liver dysfunction.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"269-277"},"PeriodicalIF":2.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vit D mitigates metabolic syndrome-induced hepatic damage via antioxidant, anti-inflammatory, and AMPK/PI3K signaling pathways in rats. 维生素D通过抗氧化、抗炎和AMPK/PI3K信号通路减轻大鼠代谢综合征诱导的肝损伤。

IF 2.7 4区医学

Archives of Physiology and Biochemistry Pub Date : 2026-06-01 Epub Date: 2025-10-21 DOI: 10.1080/13813455.2025.2573343

Suzan A Khodir, Eman M Sweed, Nahla M Badr, Nader G Zaki, Marwa M Khalil, Marwa M Adel

{"title":"Vit D mitigates metabolic syndrome-induced hepatic damage via antioxidant, anti-inflammatory, and AMPK/PI3K signaling pathways in rats.","authors":"Suzan A Khodir, Eman M Sweed, Nahla M Badr, Nader G Zaki, Marwa M Khalil, Marwa M Adel","doi":"10.1080/13813455.2025.2573343","DOIUrl":"10.1080/13813455.2025.2573343","url":null,"abstract":"Background: Metabolic syndrome (MetS) involves insulin resistance and dyslipidemia.Objective: To evaluate vitamin D's (Vit D) hepatoprotective role in MetS and its mechanisms.Methods: Thirty male rats were divided into MetS, MetS + Vit D, and control groups. We measured arterial blood pressure (ABP), body weight, fat, homeostatic Model Assessment for Insulin Resistance (HOMA-IR), glycemic/lipid profiles, liver enzymes, oxidative stress (Malondialdehyde, superoxide dismutase), inflammation (Tumor necrosis factor; TNF-α, matrix metalloproteinase; MMP9, Glial fibrillary acidic protein; GFAP), and AMP-activated protein kinase/Phosphoinositide 3-kinase (AMPK/PI3K) expression.Results: MetS increased oxidative stress, TNF-α, liver enzymes, HOMA-IR, and ABP while reducing adiponectin and AMPK/PI3K. Vit D reversed these effects, suppressing GFAP/MMP9 and restoring metabolic balance. Conclusion: Vit D mitigates MetS-induced liver damage via AMPK/PI3K activation, exerting antioxidant and anti-inflammatory effects.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"288-304"},"PeriodicalIF":2.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145336331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circular RNA ribosomal protein L15 accelerates epithelial mesenchymal transformation and tumour growth in non-small cell lung carcinoma via modulating the microRNA-518c-3p/WD repeat protein 1 axis. 环状RNA核糖体蛋白L15通过调节microRNA-518c-3p/WD重复蛋白1轴加速非小细胞肺癌上皮间质转化和肿瘤生长。

IF 2.7 4区医学

Archives of Physiology and Biochemistry Pub Date : 2026-06-01 Epub Date: 2025-11-07 DOI: 10.1080/13813455.2025.2578747

Gen Hu, Yun Ge, Bo Chen, Songchun Liu, Tao Wang

{"title":"Circular RNA ribosomal protein L15 accelerates epithelial mesenchymal transformation and tumour growth in non-small cell lung carcinoma via modulating the microRNA-518c-3p/WD repeat protein 1 axis.","authors":"Gen Hu, Yun Ge, Bo Chen, Songchun Liu, Tao Wang","doi":"10.1080/13813455.2025.2578747","DOIUrl":"10.1080/13813455.2025.2578747","url":null,"abstract":"Objective: This study aimed to investigate the effects of circ-RPL15 on epithelial mesenchymal transformation (EMT) and tumour growth in non-small cell lung cancer (NSCLC).Methods: circ-RPL15, microRNA (miR)-518c-3p and WD repeat protein 1 (WDR1) in tumour tissues were detected. Cell viability, apoptosis, migration and invasion, and EMT were analysed in A549 cells after altering circ-RPL15 or miR-518c-3p expression. The targeting relationship between miR-518c-3p with circ-RPL15 or WDR1 was verified.Results: The results elucidated that circ-RPL15 and WDR1 levels were elevated in NSCLC tissues, while miR-518c-3p was downregulated. Suppressing circ-RPL15 or elevating miR-518c-3p suppressed cell growth and delayed EMT process. miR-518c-3p was found to target both circ-RPL15 and WDR1, and increasing WDR1 counteracted the effects of reducing circ-RPL15 on A549 cells. Downregulating circ-RPL15 suppressed tumour growth in NSCLC in vivo.Conclusion: Shortly, circ-RPL15 accelerates EMT and tumour growth in NSCLC via modulating the miR-518c-3p/WDR1 axis.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"305-318"},"PeriodicalIF":2.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145457413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Menthol ameliorates thioacetamide-induced renal fibrosis in rats by upregulating SIRT1/Nrf2 and downregulating TGF-β1/Smad3 signalling pathways. 薄荷醇通过上调SIRT1/Nrf2和下调TGF-β1/Smad3信号通路改善硫代乙酰胺诱导的大鼠肾纤维化。

IF 2.7 4区医学

Archives of Physiology and Biochemistry Pub Date : 2026-06-01 Epub Date: 2025-09-02 DOI: 10.1080/13813455.2025.2554677

Elaheh Babaei, Masoumeh Asle-Rousta, Sanaz Mahmazi

{"title":"Menthol ameliorates thioacetamide-induced renal fibrosis in rats by upregulating SIRT1/Nrf2 and downregulating TGF-β1/Smad3 signalling pathways.","authors":"Elaheh Babaei, Masoumeh Asle-Rousta, Sanaz Mahmazi","doi":"10.1080/13813455.2025.2554677","DOIUrl":"10.1080/13813455.2025.2554677","url":null,"abstract":"Introduction: Renal fibrosis is a significant factor in the progression of chronic kidney disease. This study examined how menthol affects thioacetamide (TA)-induced biochemical, molecular, and histopathological damage that leads to renal fibrosis and dysfunction.Methods: Male Wistar rats were treated with TA (200 mg/kg, intraperitoneally) twice a week for four consecutive weeks, along with menthol (10 mg/kg, intraperitoneally) for the same duration.Results: Menthol effectively reduced oxidative stress and inflammation in the kidneys of rats treated with TA. It also lowered the expression of TGF-β1, SMAD3, α-SMA, and KIM-1. Furthermore, menthol prevented the decline in SIRT1 mRNA expression and protein levels while increasing the expression of Nrf2. It inhibited collagen deposition and histological damage in the kidneys and prevented the rise in serum creatinine and BUN levels.Conclusion: Menthol provides protective effects against renal fibrosis induced by thioacetamide. Its antifibrotic effects are mediated by upregulating SIRT1/Nrf2 and downregulating TGF-β1/Smad3 pathways.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"255-268"},"PeriodicalIF":2.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144940472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0