Archives of Physiology and Biochemistry最新文献

{"title":"Protective effects of <i>Cuscuta chinensis</i> Lam. extract against learning and memory dysfunction induced by streptozotocin and amyloid β_25-35 <i>in vivo</i> model.","authors":"Ji-Hyun Kim, Hyo Jeong Seo, Byeong Wook Noh, Mei Tong He, Yung-Hyun Choi, Eun Ju Cho, Jeong Sook Noh","doi":"10.1080/13813455.2025.2502861","DOIUrl":"https://doi.org/10.1080/13813455.2025.2502861","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is associated with hyperglycaemia and amyloid beta (Aβ) accumulation. In the present study, we investigated whether an aqueous extract of <i>Cuscuta chinensis</i> Lam. (CCWE) improved cognitive disorder in a hyperglycaemic and cognitive-impaired mouse model. Hyperglycaemia was induced by streptozotocin (STZ, 50 mg/kg) and a single intracerebroventricular injection of Aβ_25-35 (25 nM) was performed. The Aβ_25-35-injected hyperglycaemic mice were then administered CCWE (100 or 200 mg/kg/day) for 14-d. The protective effects of the CCWE were evaluated by behavioural tests and western blot analysis. The bioactive compounds in CCWE were isolated by UPLC-QTOF/MS analysis. The administration of CCWE improved the learning and memory function in STZ/Aβ_25-35-injected mice. Moreover, CCWE positively regulated the amyloidogenic pathway-related proteins and insulin signalling-related proteins. The bioactive components in CCWE were also identified. These findings suggest the possibility of CCWE as a potential candidate for the dual-targeting treatment of hyperglycaemia and AD.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-13"},"PeriodicalIF":2.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preconceptional treatment with açaí juçara (<i>Euterpe edulis</i> Martius) in advanced-age female rats did not interfere with metabolic parameters and redox balance during pregnancy.","authors":"Mayra Dias Rodrigues, Brenda Francisconi Diaz, Fernando Henrique Borges, Rhauany Pelisson Guergolette, Larissa Rugila Dos Santos Stopa, Ernane Torres Uchoa, Glaura Scantamburlo Alves Fernandes, Karla Bigetti Guergoletto, Graziela Scalianti Ceravolo","doi":"10.1080/13813455.2025.2497260","DOIUrl":"https://doi.org/10.1080/13813455.2025.2497260","url":null,"abstract":"<p><p>The consumption of açaí has been shown to be beneficial to health. This study aimed to evaluate the effects of juçara açai (JU, <i>Euterpe edulis Martius</i>) administration before gestation on the biometric, metabolic, and oxidative status in pregnant rats of advanced maternal age. Healthy female Wistar rats were treated with JU pulp via gavage from postnatal day 168 to 210. After treatment, the rats were mated, and during the 20 days of pregnancy, they were evaluated for body weight (BW), glucose tolerance, adipose tissue and liver weight, the lipid profile, and hepatic oxidative status. At birth, the offspring were weighed and counted. It was observed that JU reduced just maternal glycaemia. The maternal preconceptional treatment did not affect offspring BW. These results suggest that JU could be a safe nutritional intervention during the preconception period in advanced maternal age.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-9"},"PeriodicalIF":2.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meteorin-like protein (Metrnl): a key exerkine in exercise-mediated cardiovascular health.","authors":"Hamid Alizadeh, Ahmad Parsaeifar, Roohollah Mohammadi Mirzaei","doi":"10.1080/13813455.2025.2497272","DOIUrl":"https://doi.org/10.1080/13813455.2025.2497272","url":null,"abstract":"<p><strong>Context: </strong>Cardiovascular diseases (CVDs) remain a leading global cause of mortality, necessitating non‑pharmacological interventions such as exercise. Meteorin‑like protein (Metrnl), an exercise‑induced myokine and adipokine, has emerged as a critical mediator of exercise‑mediated cardiovascular benefits, though its specific mechanisms and clinical implications remain underexplored.</p><p><strong>Objective: </strong>This review synthesizes current evidence on Metrnl's role as a key exerkine in cardiovascular health, focusing on its exercise‑induced regulatory mechanisms, tissue‑specific effects, and therapeutic potential for CVD management.</p><p><strong>Methods: </strong>A comprehensive analysis of preclinical and clinical studies was conducted, encompassing molecular, metabolic, and anti‑inflammatory pathways linked to Metrnl. Literature from PubMed, Scopus, and Web of Science was systematically reviewed to evaluate Metrnl's role in exercise‑mediated cardiovascular adaptations.</p><p><strong>Results: </strong>Exercise‑induced Metrnl enhances endothelial function, vascular remodeling, and metabolic regulation via AMPK, PPARγ, and KIT receptor signaling. It promotes glucose/lipid metabolism, angiogenesis, and anti‑inflammatory responses, reducing atherosclerotic risks and improving cardiac repair post‑infarction. Clinically, Metrnl levels correlate with CVD severity, acting as a biomarker for risk stratification. Acute exercise elevates Metrnl, while chronic training effects vary by modality and population. Paradoxically, elevated plasma Metrnl in acute cardiac events predicts adverse outcomes, whereas reduced levels in chronic conditions (e.g., diabetes, heart failure) reflect metabolic dysregulation.</p><p><strong>Discussion: </strong>Metrnl bridges exercise benefits to cardiovascular health through inter‑organ crosstalk, yet discrepancies exist in its chronic exercise‑mediated regulation. Its dual role as a protective mediator and stress‑responsive biomarker underscores context‑dependent interpretations. Unresolved questions include receptor specificity, tissue autonomy, and therapeutic delivery strategies.</p><p><strong>Conclusion: </strong>Metrnl is a pivotal exerkine with promising diagnostic and therapeutic potential for CVDs. Translating its exercise‑mediated benefits into clinical applications requires further human trials to validate mechanisms and optimize interventions. Harnessing Metrnl could revolutionize strategies for CVD prevention and rehabilitation, leveraging exercise's molecular advantages.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-15"},"PeriodicalIF":2.5,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143966131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum levels of miR-34, miR-182 and miR-378 as novel diagnostic biomarkers in Behçet patients and their relation to disease activity and severity.","authors":"Miran M Harhash, Amr A Zahra, Omayma O Abdelaleem, Nermeen A Fouad, Hassan S El Sayed","doi":"10.1080/13813455.2025.2497266","DOIUrl":"https://doi.org/10.1080/13813455.2025.2497266","url":null,"abstract":"<p><strong>Background: </strong>Behçet Disease (BD) is a chronic multi-systemic vasculitis of relapsing and remitting nature. Many recent studies have denoted the role of micro RNAs (MiRNAs) in the pathogenesis of BD.</p><p><strong>Subjects and methods: </strong>Blood samples were withdrawn from 50 BD patients and 40 age and sex-matched healthy individuals in this study.</p><p><strong>Results: </strong>Serum expression levels of miR-34a and miR-182 were significantly elevated in BD patients when compared to controls, <i>p</i> < .001. However, serum expression levels of miR-378 were significantly decreased in BD patients compared to controls, <i>p</i> < .001. miR-182 serum levels were also found to be elevated in active BD patients compared to patients in inactive state (<i>p</i> = .022). We found a significant association between miR-34 levels and joint affection in BD patients as well as a significant relation between miR-182 levels and each of neurological manifestations and genital ulcerations. In addition, a statistically significant positive correlations were proved in the current results between miR-182 expression and BDCAF score (<i>r</i> = 0.419, <i>p</i> = .002) as well as severity score (<i>r</i> = 0.358, <i>p</i> = .011).</p><p><strong>Conclusion: </strong>Our study denoted that the three miRNAs; miR-34a, miR-182, and miR-378 possibly play a crucial role in the pathogenesis of BD. The distinction of their serum levels between patients and healthy individuals suggested their potentiality as promising biomarkers for BD.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-10"},"PeriodicalIF":2.5,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective role of butylated hydroxyanisole (BHA) and hydroxytoluene (BHT) against oxidative stress-induced inflammatory response in carbon tetrachloride-induced acute hepatorenal toxicity.","authors":"Barsha Dassarma, Santanu Kar Mahapatra, Dilip Kumar Nandi, Somnath Gangopadhyay, Saptadip Samanta","doi":"10.1080/13813455.2025.2493105","DOIUrl":"https://doi.org/10.1080/13813455.2025.2493105","url":null,"abstract":"<p><strong>Background: </strong>Any toxicity initially damages the hepatic system, followed by renal dysfunction. Previously, it was established that carbon tetrachloride (CCl₄) intoxication severely damaged hepatocytes. Moreover, CCl₄-mediated toxicity significantly impacted immune functions and influenced the inflammatory response, with mitochondrial dysfunction. The present study focused on the levels of inflammatory markers and mitochondrial dysfunction, as well as the protective role of BHA and BHT.</p><p><strong>Methods: </strong>In the present study, hepatorenal dysfunction was developed in experimental rats by applying a subcutaneous injection of CCl₄ with a dose of 230 mg/kg bwt/rat/day. The level of immune toxicity was determined by measuring C-reactive protein (CRP), IL-6, 12, TNF-α, IL-10, and TGF-β in CCl4 intoxicated group and pretreated BHA and BHT groups. ROS generation and MMP were also measured in hepatic and renal cells using flow cytometric technique.</p><p><strong>Results: </strong>The level of toxicity was determined by a significant increase of CRP (407.29%), IL-6 (525.65%), IL-12 (1026.54%), and TNF-α (1007.33%) in CCl₄ intoxicated group, while IL-10 and TGF-β were significantly decreased 84.65% and 66.36%, respectively. CCl₄ intoxication caused decreased mitochondrial membrane potential and high levels of intracellular ROS generation. Pretreatment with BHA (0.5 mg/kg/bwt) and BHT (0.8 mg/kg/bwt) significantly (<i>p</i><0.001, <i>p</i><0.05) reduced inflammatory markers in the CCl4-treated group, restored mitochondrial membrane potential and decreased intracellular ROS levels.</p><p><strong>Conclusion: </strong>BHA and BHT treatment could restrict the higher concentration of pro-inflammatory markers by scavenging ROS. Therefore, the study suggested that supplementation of BHA and BHT could be an alternative treatment for preventing hepatorenal dysfunctions.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-8"},"PeriodicalIF":2.5,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of herbaceous bioflavonoid herbacetin on oxidative stress, and alpha-synuclein regulation, programmed cell death in a Parkinson illness.","authors":"Chinnadurai Veeramani, Mohammed A Alsaif, Muhammad Ibrar Khan, Ahmed S El Newehy, Ali Alshammari, Khalid S Al-Numair","doi":"10.1080/13813455.2025.2493103","DOIUrl":"https://doi.org/10.1080/13813455.2025.2493103","url":null,"abstract":"<p><strong>Background: </strong>Herbacetin, a flavonoid present in many types of herbs, which include linaceae, ephedraceae, and crassulaceae, exhibits a range of medicinal properties. 1-methyl-4-phenylpyridinium (MPP⁺) is one of the neurotoxins used in cell-based Parkinson's disease (PI) models. Whereas the precise chemical mechanism of iron association with free radical cell damage and apoptosis is yet unknown, intracellular irons are a key factor for MPP⁺-derived apoptosis.</p><p><strong>Methods: </strong>We examine whether the antiapoptotic properties of flaxseed bioflavonoid herbacetin (HB) are associated with the stimulation of the intrinsic caspase-dependent pathway and exposing of MPP⁺ caused neuronal death in the human dopaminergic neuroblastoma cells. Four groups were created out of the cells. Groups I, II, III, and IV are the control, HB+MPP⁺, MPP⁺, and HB, respectively. Following a 24-hour incubation period, the cells were subjected to several parameters.</p><p><strong>Results: </strong>We discovered in neuroblastoma cells that HB dramatically reduced the cell death induced by MPP⁺. Additionally, HB significantly reduced the formation of ROS and counteracted the reduction in MMP resulting from MPP⁺ treatment. HB reduces the stimulation of the intrinsic caspase-dependent apoptotic mechanism and suppresses the MPP⁺-mediated apoptotic signalling pathway. Furthermore, HB predicted a better binding interaction with alpha-synuclein and drastically decreased alpha-synuclein expression and accumulation in neuroblastoma cells.</p><p><strong>Conclusion: </strong>Consequently, our findings imply that HB shields neurons by reducing oxidative stress, alpha-synuclein misfolding in neuroblastoma, and apoptosis prompts the death of neuroblastoma cells.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-12"},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between MTHFR 677C > T polymorphism and serum PIVKA-II levels in hepatocellular carcinoma.","authors":"Hongyu Zhang, Baixiu Wu, Liang Zhang, Zheng Peng","doi":"10.1080/13813455.2025.2493107","DOIUrl":"https://doi.org/10.1080/13813455.2025.2493107","url":null,"abstract":"<p><strong>Background: </strong>Hepatocellular carcinoma (HCC) is a major public health problem with increasing incidence and mortality worldwide. The methylenetetrahydrofolate reductase (MTHFR) 677 C > T polymorphism is associated with the development and progression of various tumours, while protein induced by vitamin K absence II (PIVKA-II) is an important tumour marker for the diagnosis of HCC. This study aims to investigate the relationship between the MTHFR 677 C > T polymorphism and serum PIVKA-II levels in HCC patients, providing new insights for early diagnosis, risk assessment, and prognosis evaluation of HCC.</p><p><strong>Methods: </strong>This study included 120 HCC patients and 100 healthy controls. MTHFR 677 C > T genotyping was performed using fluorescent quantitative PCR, and serum PIVKA-II levels were measured. Bioinformatics analysis was used to explore the expression of the MTHFR gene in HCC and its relationship with prognosis.</p><p><strong>Results: </strong>MTHFR 677 C > T TT carriers had an increased risk of HCC (OR = 2.393; 95% CI 1.055-5.429; <i>p</i> = 0.037); the risk of HCC for T gene carriers was 58.3% higher than that for C gene carriers in the allele model (OR = 1.583; 95% CI 1.059-2.364; <i>p</i> = 0.025). The difference in serum PIVKA-II concentration was statistically significant between the controls, stage I-II patients, and stage III-IV patients (<i>p</i> < 0.05), and the difference in serum PIVKA-II concentration was statistically significant between patients with the TT genotype and patients with the CC and CT genotypes (all <i>p</i> values less than 0.05). UALCAN database analysis showed that MTHFR gene expression levels were increased in patients with HCC, and the high expression of the MTHFR gene was negatively correlated with patient survival rates.</p><p><strong>Conclusions: </strong>There is an association between the MTHFR 677 C > T TT genotype and serum PIVKA-II levels in HCC. This could help identify high-risk individuals and assess disease severity, providing a potential genetic biomarker for the diagnosis of HCC.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-8"},"PeriodicalIF":2.5,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum levels of omentin and visfatin in patients with metabolic syndrome.","authors":"Anita Sherly A, Rukmini M S, Anupama Hegde, Arun S, Himani Kotian","doi":"10.1080/13813455.2025.2486290","DOIUrl":"10.1080/13813455.2025.2486290","url":null,"abstract":"<p><strong>Introduction: </strong>Metabolic Syndrome (MetS) is a global health concern characterised by cardiometabolic risk factors, dysregulated adipokine signalling and inflammation. The study aimed to assess the serum levels of omentin and visfatin in patients with metabolic syndrome.</p><p><strong>Methods: </strong>The 84-subject hospital-based case-control study included 18-55 years, both genders. Anthropometry, medical history, fasting plasma glucose (FPG), lipid profile, and HOMA-IR were collected. Insulin, omentin, and visfatin were measured using ELISA.</p><p><strong>Results: </strong>Omentin and visfatin levels significantly differed between groups (<i>p</i> < 0.05). The median omentin levels were 50.74 and 45.25; visfatin levels were 0.064 and 0.001, respectively. Omentin correlated with waist circumference, blood pressure, and FPG in controls, while visfatin correlated with HDL and BMI among cases (<i>p</i> < 0.05). Omentin and visfatin were elevated in cases. However, no significant correlation between omentin and visfatin with lipid parameters could be established.</p><p><strong>Conclusion: </strong>Omentin and visfatin levels varied significantly between metabolic syndrome and controls; their correlation with MetS criteria was not significant.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-8"},"PeriodicalIF":2.5,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of elevated levels of the gut metabolite TMAO on glucose metabolism after sleeve gastrectomy.","authors":"Zhiping Huang, Chaoqian Liu, Xiang Zhao, Yan Guo","doi":"10.1080/13813455.2025.2489721","DOIUrl":"10.1080/13813455.2025.2489721","url":null,"abstract":"<p><p><b>Purpose：</b>Bariatric surgery can effectively alleviate obesity and diabetes by regulation of the gut microbiota. This study aimed to investigate the change in the gut microbiota metabolite TMAO and to explore its effect on glucose metabolism after sleeve gastrectomy (SG).</p><p><p><b>Materials and methods：</b>Diet-induced obesity mouse models were established, and the mice were randomly divided into four groups: an SG group, a sham-operated group pair-fed with the SG group (PF), a sham-operated group fed ad libitum (AL), and a lean control group (C). At 10 weeks post-surgery, the changes in glycogen content of liver, gut microbiota and the level of FMO3 in the liver were evaluated, and their correlation with TMAO production was analysed. The expression levels of the TMAO/PERK/FOXO1 pathway and the gluconeogenic genes G6PC and PCK1 were measured.</p><p><p><b>Results：</b>At 10 weeks post-surgery, hepatocyte glycogen levels were restored, and serum TMA and TMAO levels were significantly increased. Faecal metagenomic sequencing results showed that the abundances of Ruminococcaceae and Lachnospiraceae, which were positively correlated with TMAO production, were significantly increased after surgery. While the changes in FMO3, the key enzyme producing TMAO in the liver was found decreased significantly after SG. The expression levels of the TMAO/PERK/FOXO1 pathway and the gluconeogenic genes G6PC and PCK1 were measured. Inconsistent with the changing trend of TMAO, the expression of PERK, FOXO1, PCK, and G6PC significantly decreased after SG.</p><p><p><b>Conclusions：</b>SG can significantly reduce obesity and restore glucose metabolism. After surgery, TMAO metabolites increased in a microbiota-dependent manner.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-10"},"PeriodicalIF":2.5,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin D₃ administration ameliorates the anxiety and depressive-like behaviour induced by nicotine withdrawal: a mechanistic focus on oxidative stress, inflammatory response, and serotonergic transmission.","authors":"Farimah Beheshti, Mehrnoush Goudarzi, Samaneh Kakhki, S Mohammad Ahmadi-Soleimani, Mustafa Ansari, Hassan Azhdari-Zarmehri","doi":"10.1080/13813455.2025.2483508","DOIUrl":"https://doi.org/10.1080/13813455.2025.2483508","url":null,"abstract":"<p><strong>Background: </strong>The present study conducted to assess whether vitamin D₃ (Vit D) could ameliorate the anxiety and depression induced by nicotine (Nic) withdrawal in male adult rats.</p><p><strong>Methods: </strong>To this end, behavioural tests were done in male Wistar rats undergone adolescent Nic exposure (2 mg/kg) and then withdrawal and the effect of Vit D (100, 1000, and 10,000 IU/kg) was assessed at both behavioural and biochemical levels.</p><p><strong>Results: </strong>Results indicated that Vit D treatment could effectively prevent anxiety, depression, and biochemical alterations induced by Nic withdrawal.</p><p><strong>Conclusion: </strong>Vit D has strong potential to be used for prevention of anxiety- and depressive-like behaviours following Nic withdrawal; however, further investigation is needed in larger sample size to discuss more confidently.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-10"},"PeriodicalIF":2.5,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}