Archives of Physiology and Biochemistry最新文献

The effect of elevated levels of the gut metabolite TMAO on glucose metabolism after sleeve gastrectomy.

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-04-09 DOI: 10.1080/13813455.2025.2489721

Zhiping Huang, Chaoqian Liu, Xiang Zhao, Yan Guo

{"title":"The effect of elevated levels of the gut metabolite TMAO on glucose metabolism after sleeve gastrectomy.","authors":"Zhiping Huang, Chaoqian Liu, Xiang Zhao, Yan Guo","doi":"10.1080/13813455.2025.2489721","DOIUrl":"https://doi.org/10.1080/13813455.2025.2489721","url":null,"abstract":"Purpose：Bariatric surgery can effectively alleviate obesity and diabetes by regulation of the gut microbiota. This study aimed to investigate the change in the gut microbiota metabolite TMAO and to explore its effect on glucose metabolism after sleeve gastrectomy (SG).Materials and methods：Diet-induced obesity mouse models were established, and the mice were randomly divided into four groups: an SG group, a sham-operated group pair-fed with the SG group (PF), a sham-operated group fed ad libitum (AL), and a lean control group (C). At 10 weeks post-surgery, the changes in glycogen content of liver, gut microbiota and the level of FMO3 in the liver were evaluated, and their correlation with TMAO production was analysed. The expression levels of the TMAO/PERK/FOXO1 pathway and the gluconeogenic genes G6PC and PCK1 were measured.Results：At 10 weeks post-surgery, hepatocyte glycogen levels were restored, and serum TMA and TMAO levels were significantly increased. Faecal metagenomic sequencing results showed that the abundances of Ruminococcaceae and Lachnospiraceae, which were positively correlated with TMAO production, were significantly increased after surgery. While the changes in FMO3, the key enzyme producing TMAO in the liver was found decreased significantly after SG. The expression levels of the TMAO/PERK/FOXO1 pathway and the gluconeogenic genes G6PC and PCK1 were measured. Inconsistent with the changing trend of TMAO, the expression of PERK, FOXO1, PCK, and G6PC significantly decreased after SG.Conclusions：SG can significantly reduce obesity and restore glucose metabolism. After surgery, TMAO metabolites increased in a microbiota-dependent manner.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-10"},"PeriodicalIF":2.5,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vitamin D₃ administration ameliorates the anxiety and depressive-like behaviour induced by nicotine withdrawal: a mechanistic focus on oxidative stress, inflammatory response, and serotonergic transmission.

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-04-08 DOI: 10.1080/13813455.2025.2483508

Farimah Beheshti, Mehrnoush Goudarzi, Samaneh Kakhki, S Mohammad Ahmadi-Soleimani, Mustafa Ansari, Hassan Azhdari-Zarmehri

引用次数: 0

GC-MS analysis, the in vitro and in vivo protective effect of Phlogacanthus thyrsiflorus Nees. on hyperglycaemia-induced diabetic mice model.

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-04-04 DOI: 10.1080/13813455.2025.2483501

Jutishna Bora, Sumira Malik, Richa Mishra, Sarvesh Rustagi, Petr Slama, Smita Lata, Nayan Talukdar, Saad Alghamdi, Abdullah Aldairi, Faten Noor Habiballah, Mazen Almehmadi, Osama Abdulaziz, Naif Alsiwiehri, Seema Ramniwas

{"title":"GC-MS analysis, the in vitro and in vivo protective effect of Phlogacanthus thyrsiflorus Nees. on hyperglycaemia-induced diabetic mice model.","authors":"Jutishna Bora, Sumira Malik, Richa Mishra, Sarvesh Rustagi, Petr Slama, Smita Lata, Nayan Talukdar, Saad Alghamdi, Abdullah Aldairi, Faten Noor Habiballah, Mazen Almehmadi, Osama Abdulaziz, Naif Alsiwiehri, Seema Ramniwas","doi":"10.1080/13813455.2025.2483501","DOIUrl":"https://doi.org/10.1080/13813455.2025.2483501","url":null,"abstract":"Objective: This study investigates the in-vitro and in-vivo antioxidant capacities showed by Phlogacanthus thyrsiflorus Nees. (P. thyrsiflorus) in alloxan-administered diabetic mice.Materials and Methods: The screening of phytochemical of methanolic flower extract (MFE), Gas Chromatography Mass Spectrometry (GC-MS) profiling was utilized to identify bioactive compounds. In-vitro antioxidant studies were performed. Acute toxicity was evaluated in mice. Glucose Transporter type 4 (GLUT4) protein expression and antioxidant enzyme activities were assessed. Histopathological examination of heart tissue was performed.Results: GC-MS analysis revealed the presence of various bioactive compounds consisting of antioxidant, anti-inflammatory activities. The results also showed a noteworthy increase in in-vitro and in-vivo antioxidant enzymes activities. Moreover, MFE suppress hyperglycaemia by upregulating GLUT4 protein expression. In histological study MFE was found to restore cellular alterations in diabetic tissue.Discussion and Conclusion: It is inferred from the study that MFE of P. thyrsiflorus can exert a protective effect by suppressing hyperglycaemia and modulating oxidative stress in alloxan-administered diabetic mice.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-12"},"PeriodicalIF":2.5,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination therapy of systemic and local metformin improves imiquimod-induced psoriasis-like lesions with type 2 diabetes: the role of AMPK/KGF/STAT3 axis. 全身和局部二甲双胍联合疗法可改善咪喹莫特诱导的 2 型糖尿病银屑病样皮损：AMPK/KGF/STAT3 轴的作用。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-04-01 Epub Date: 2024-10-24 DOI: 10.1080/13813455.2024.2407547

Fatma ElSayed Hassan, Basma Emad Aboulhoda, Marwa Nagi Mehesen, Passant Mohie El Din, Hend Ahmed Abdallah, Ehab R Bendas, Laila Ahmed Rashed, Abeer Mostafa, Marwa Fathy Amer, Marwa Abdel-Rahman, Mansour A Alghamdi, Asmaa Mohammed Shams Eldeen

{"title":"Combination therapy of systemic and local metformin improves imiquimod-induced psoriasis-like lesions with type 2 diabetes: the role of AMPK/KGF/STAT3 axis.","authors":"Fatma ElSayed Hassan, Basma Emad Aboulhoda, Marwa Nagi Mehesen, Passant Mohie El Din, Hend Ahmed Abdallah, Ehab R Bendas, Laila Ahmed Rashed, Abeer Mostafa, Marwa Fathy Amer, Marwa Abdel-Rahman, Mansour A Alghamdi, Asmaa Mohammed Shams Eldeen","doi":"10.1080/13813455.2024.2407547","DOIUrl":"10.1080/13813455.2024.2407547","url":null,"abstract":"Context: Insulin resistance and a disturbed lipid profile are common associations with type 2 diabetes mellitus (T2DM) and different skin diseases, particularly psoriasis (PsO).Objectives: We investigated potential therapeutic mechanisms of metformin in a murine animal model of psoriasiform lesions in T2DM.Materials and methods: Forty-two rats were randomly divided into control, PsO, and type II DM (T2DM) groups. After confirmation of DM, the type II diabetic rats were allocated into T2DM+ PsO, T2DM+ PsO+ systemic metformin (S. met), T2DM+ PsO+ topical metformin (T. met)), and T2DM+ PsO + combined metformin (C. met). PsO was induced by topical imiquimod.Results: Systemic administration of the cornerstone antidiabetic drug, metformin, was able to improve insulin resistance and lipid profile. At molecular levels, both topical and systemic metformin significantly increased AMP-activated protein kinase (AMPK), and lowered keratinocyte growth factor (KGF) / \"Signal transducer and activator of transcription\" (STAT)3 protein levels, and the IL-17RA and IL-17RC gene expression.Conclusion: Although its glucose-controlling effect was not optimum, T.met gel served anti-psoriatic and anti-inflammatory effects.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"252-264"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of Helicobacter pylori-derived extracellular vesicles on glucose metabolism and induction of insulin resistance in HepG2 cells. 幽门螺杆菌衍生的细胞外囊泡对 HepG2 细胞葡萄糖代谢和诱导胰岛素抵抗的影响

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-04-01 Epub Date: 2024-10-21 DOI: 10.1080/13813455.2024.2418494

Ghazaleh Talebi, Parvaneh Saffarian, Mojdeh Hakemi-Vala, Amir Sadeghi, Abbas Yadegar

{"title":"The effect of Helicobacter pylori-derived extracellular vesicles on glucose metabolism and induction of insulin resistance in HepG2 cells.","authors":"Ghazaleh Talebi, Parvaneh Saffarian, Mojdeh Hakemi-Vala, Amir Sadeghi, Abbas Yadegar","doi":"10.1080/13813455.2024.2418494","DOIUrl":"10.1080/13813455.2024.2418494","url":null,"abstract":"Helicobacter pylori infection has been associated with the development of insulin resistance (IR). This study aimed to examine the effect of H. pylori-derived extracellular vesicles (EVs) on IR induction. EVs were derived from two H. pylori strains, and characterised by transmission electron microscopy and dynamic light scattering. Different concentrations of insulin were added to HepG2 cells to induce IR model. HepG2 cells were exposed to various concentrations of H. pylori-derived EVs to assess IR development. The gene expression of IRS1, AKT2, GLUT2, IL-6, SOCS3, c-Jun and miR-140 was examined using RT-qPCR. Glucose uptake analysis revealed insulin at 5 × 10 -7 mol/l and EVs at 50 µg/ml induced IR model in HepG2 cells. H. pylori-derived EVs downregulated the expression level of IRS1, AKT2, and GLUT2, and upregulated IL-6, SOCS3, c-Jun, and miR-140 expression in HepG2 cells. In conclusion, our findings propose a novel mechanism by which H. pylori-derived EVs could potentially induce IR.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"316-327"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of exogenous ghrelin treatment on oxidative stress, inflammation and histological parameters in a fat-fed streptozotocin rat model. 外源性胃泌素对脂肪喂养链脲佐菌素大鼠模型氧化应激、炎症和组织学参数的影响

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-04-01 Epub Date: 2024-09-26 DOI: 10.1080/13813455.2024.2407551

Ozlem Ergul Erkec, Zubeyir Huyut, Eda Acikgoz, Mehmet Tahir Huyut

{"title":"Effects of exogenous ghrelin treatment on oxidative stress, inflammation and histological parameters in a fat-fed streptozotocin rat model.","authors":"Ozlem Ergul Erkec, Zubeyir Huyut, Eda Acikgoz, Mehmet Tahir Huyut","doi":"10.1080/13813455.2024.2407551","DOIUrl":"10.1080/13813455.2024.2407551","url":null,"abstract":"In this study, the anti-inflammatory, antioxidative, and protective effects of ghrelin were investigated in a fat-fed streptozotocin (STZ) rat model and compared with metformin, diabetes and the healthy control groups. Histopathological evaluations were performed on H&E-stained pancreas and brain sections. Biochemical parameters were investigated by enzyme-linked immunosorbent assay. Blood glucose levels were significantly decreased with ghrelin or metformin treatments than the diabetes group. STZ administration increased brain, renal and pancreatic IL-1β, TNF-α and MDA while decreasing GPX, CAT, SOD, and NGF levels. Ghrelin increased renal GPX, CAT, NGF pancreatic GPX, SOD, CAT, NGF and brain SOD, NGF while it decreased renal, pancreatic and brain IL-1β, TNF-α and MDA levels. Ghrelin reduced neuronal loss and degeneration in the cerebral cortex and hippocampus and greatly ameliorated diabetes-related damage in pancreas. In conclusion, the data suggested that ghrelin is an effective candidate as a protectant for reducing the adverse effects of diabetes.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"274-284"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 更正。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-04-01 Epub Date: 2024-10-22 DOI: 10.1080/13813455.2024.2418702

引用次数: 0

Artichoke leaf hydroethanolic extract reduces neuropathic pain in a rat model of chronic constriction injury via attenuating the sciatic nerve oxidative stress. 朝鲜蓟叶水乙醇提取物可通过减轻坐骨神经氧化应激减轻慢性收缩损伤模型大鼠的神经痛。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-04-01 Epub Date: 2024-09-25 DOI: 10.1080/13813455.2024.2406898

Mohammad Mehdi Haghighat Lari, Mohammad Reza Bakhoda, Mohammad Shabani, Mohsen Taghizadeh, Fereshteh Bahmani, Gholamali Hamidi, Fatemeh Aghighi, Sayyed Alireza Talaei

{"title":"Artichoke leaf hydroethanolic extract reduces neuropathic pain in a rat model of chronic constriction injury via attenuating the sciatic nerve oxidative stress.","authors":"Mohammad Mehdi Haghighat Lari, Mohammad Reza Bakhoda, Mohammad Shabani, Mohsen Taghizadeh, Fereshteh Bahmani, Gholamali Hamidi, Fatemeh Aghighi, Sayyed Alireza Talaei","doi":"10.1080/13813455.2024.2406898","DOIUrl":"10.1080/13813455.2024.2406898","url":null,"abstract":"Neuropathic pain, a nerve damage consequence, presents symptoms such as dysesthesia, hyperalgesia, and allodynia. This study aimed to evaluate the alleviating potential of artichoke leaf extract in neuropathic pain induced by chronic constriction injury (CCI) of the sciatic nerve in male rats. The hydroethanolic extract of artichoke leaf was administered via gavage at doses of 200, 400, and 800 mg/kg for 21 days. Behavioural tests were conducted on days 1, 4, 7, 14, and 21 post-surgeries. Only the dose of 800 mg/kg significantly reduced thermal hyperalgesia and allodynia from day 14 and mechanical allodynia from day 7, and the other doses did not affect behaviours. Biochemical analysis showed that artichoke extract decreased lipid peroxidation and restored antioxidant enzyme activities (SOD and GPx) in the sciatic nerve tissue. In conclusion, artichoke leaf extract administration diminishes neuropathic pain-related behaviours by enhancing antioxidant capacity and reducing oxidative stress in the rats' sciatic nerve.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"227-233"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Huaiqihuang (HQH) protects podocytes from high glucose-induced apoptosis and inflammation response by regulating PI3K/AKT/mTOR pathway. 怀其黄（HQH）通过调节 PI3K/AKT/mTOR 通路，保护荚膜细胞免受高糖诱导的细胞凋亡和炎症反应的影响。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-04-01 Epub Date: 2024-09-27 DOI: 10.1080/13813455.2024.2407552

Peipei Zhang, Zhilong Liu, Guiqiao Ma, Junwei Wang, Jing Shao, Chaojing Ma, Liping Wang, Chanjuan Ma

引用次数: 0

Chronic whole-body heat treatment in obese insulin-resistant C57BL/6J mice. 对肥胖的胰岛素抵抗型 C57BL/6J 小鼠进行慢性全身热处理。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-04-01 Epub Date: 2024-09-26 DOI: 10.1080/13813455.2024.2406904

Helena Trevisan Schroeder, Carlos Henrique de Lemos Muller, Maria Inês Lavina Rodrigues, Marcela Alves de Azevedo, Victor de Souza Borges, Cristiana Maria Sponchiado, Paulo Ivo Homem de Bittencourt

{"title":"Chronic whole-body heat treatment in obese insulin-resistant C57BL/6J mice.","authors":"Helena Trevisan Schroeder, Carlos Henrique de Lemos Muller, Maria Inês Lavina Rodrigues, Marcela Alves de Azevedo, Victor de Souza Borges, Cristiana Maria Sponchiado, Paulo Ivo Homem de Bittencourt","doi":"10.1080/13813455.2024.2406904","DOIUrl":"10.1080/13813455.2024.2406904","url":null,"abstract":"Aim: This study examined the effects of hyperthermic therapy (HT) on mice fed normal chow or a high-fat diet (HFD) for 18 or 22 weeks, undergoing four or eight weekly HT sessions.Methods: Mice were housed within their thermoneutral zone (TNZ) to simulate a physiological response. HFD-induced obesity-related changes, including weight gain, visceral fat accumulation, muscle loss (indicative of obesity sarcopenia), glucose intolerance, and hepatic triglyceride buildup.Main results: HT upregulated HSP70 expression in muscles, mitigated weight gain, normalised QUICK index, and reduced plasma HSP70 concentrations. It also lowered the H-index of HSP70 balance, indicating improved immunoinflammatory status, and decreased activated caspase-1 and proliferative senescence in adipose tissue, both linked to insulin resistance.Conclusion: The findings suggest that even animals on a \"control\" diet but with insufficient physical activity and within their TNZ may experience impaired glycaemic homeostasis.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"234-251"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0