Archives of Physiology and Biochemistry最新文献

Effects of 8 weeks of high-intensity interval training on hormonal and adipokines responses in people living with HIV: a non-controlled clinical trial. 8周高强度间歇训练对HIV感染者激素和脂肪因子反应的影响：一项非对照临床试验

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-07-25 DOI: 10.1080/13813455.2025.2529291

Bruno Ferrari Silva, Eloá Jacques Pastório, Gisele Cristina Bueno, Sidney Barnabé Peres, Solange Marta Franzoi de Moraes

{"title":"Effects of 8 weeks of high-intensity interval training on hormonal and adipokines responses in people living with HIV: a non-controlled clinical trial.","authors":"Bruno Ferrari Silva, Eloá Jacques Pastório, Gisele Cristina Bueno, Sidney Barnabé Peres, Solange Marta Franzoi de Moraes","doi":"10.1080/13813455.2025.2529291","DOIUrl":"https://doi.org/10.1080/13813455.2025.2529291","url":null,"abstract":"Background: To verify the effect of high-intensity interval training (HIIT) on hormonal and adipokine levels in people living with HIV (PLWH).Methods: The non-controlled clinical trial, two-arms, parallel, in 18 controls (12 women; 35.9 ± 13.3 years old; 34.5 ± 9.4% of body fat) and 19 PLWH (11 women; 41.5 ± 13.4 years old; 28.3 ± 9.1% of body fat) were allocated into separate groups without prior knowledge of the distribution. Both groups were submitted to 24 HIIT sessions of ten exercises. An incremental stress test, body composition, and blood colledtion were carried out before and after training. Data were analysed using the Shapiro-Wilk test, independent t-test, and two-way ANOVA (P < 0.05).Results: HIV- group decreased total cholesterol and triglycerides; HIV+ group decreased serum insulin, HOMA-IR index, and adiponectin; Quicky index was improved (P < 0.05).Conclusion: HIIT improves the metabolic and hormonal profile of PLWH, mainly insulin resistance and adiponectin levels.Brazilian registry of clinical trials: UTN: U1111-1231-1846.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-11"},"PeriodicalIF":2.5,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Honokiol prevents central kainic acid-induced neurodegeneration by suppressing oxidative stress, inflammation, and TGF-β1 expression. 本木酚通过抑制氧化应激、炎症和TGF-β1的表达来预防中央茴香酸诱导的神经变性。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-07-22 DOI: 10.1080/13813455.2025.2535723

Mehmet Demir, Dilan Cetinavci, Kubranur Dogan, Hulya Elbe, Ercan Saruhan

{"title":"Honokiol prevents central kainic acid-induced neurodegeneration by suppressing oxidative stress, inflammation, and TGF-β1 expression.","authors":"Mehmet Demir, Dilan Cetinavci, Kubranur Dogan, Hulya Elbe, Ercan Saruhan","doi":"10.1080/13813455.2025.2535723","DOIUrl":"https://doi.org/10.1080/13813455.2025.2535723","url":null,"abstract":"This study explored the neuroprotective effects of honokiol against oxidative stress, neuroinflammation and transforming growth factor-beta1 (TGF-β1) pathways in kainic acid (KA)-induced neurodegeneration in rats. The animals were divided into: control [Honokiol solvent (dimethyl sulphoxide), intraperitoneal for 7 days]; sham [single-dose KA solvent (saline, intracerebroventricular)]; KA (0,5 μg/μl, single-dose, intracerebroventricular); Honokiol [5 mg/kg-intraperitoneal) for 7 days]; and KA+Honokiol [KA single dose and Honokiol (for 7 days)]. Cerebral cortex and hippocampus tissues of the right hemispheres of rat brains were removed and examined biochemically and histopathologically. KA administration caused an increase in malondialdehyde levels and a decrease in reduced glutathione (GSH) and superoxide dismutase (SOD) levels. In addition, interleukin-1β levels and TGF-β1 expression were increased. Honokiol treatment decreased malondialdehyde levels, increased SOD and GSH levels, increased interleukin-1β levels and improved TGF-β1 expression in rats. Our data showed Honokiol has a protective potential against kainic acid-induced neurodegeneration by suppressing oxidative stress, inflammation and TGF-β1 expression.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-12"},"PeriodicalIF":2.5,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resistance training performed by parents modulates offspring adiposity and insulin sensitivity. 父母进行的抗阻训练调节后代的肥胖和胰岛素敏感性。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-07-21 DOI: 10.1080/13813455.2025.2533341

Fábio Henrique Dos Santos, Ana Flávia Sordi, Solange Marta Franzói De Moraes, Maria Montserrat Diaz Pedrosa, Sidney Barnabé Peres

{"title":"Resistance training performed by parents modulates offspring adiposity and insulin sensitivity.","authors":"Fábio Henrique Dos Santos, Ana Flávia Sordi, Solange Marta Franzói De Moraes, Maria Montserrat Diaz Pedrosa, Sidney Barnabé Peres","doi":"10.1080/13813455.2025.2533341","DOIUrl":"https://doi.org/10.1080/13813455.2025.2533341","url":null,"abstract":"Context: Physical exercise is one of many environmental variables that may affect an organism through epigenetic mechanisms, and thus, it may be passed on to the offspring.Objective: We assessed the effect of resistance training by the parents on mice offspring.Materials and methods: Training protocol lasted eight weeks, being males and females paired for mating. After birth, the litters were adjusted to eight pups, organised into four groups: sedentary parents (SS), trained parents (TT), sedentary fathers and trained mothers (ST), and trained fathers and sedentary mothers (TS). Male and female pups were analysed separately at the age of 21 days. One-way ANOVA or Kruskal-Wallis was applied when appropriate at the significance level of p < 0.05.Results: Resistance training improved the strength of both male and female parents. HOMA-IR index of the female offspring of groups ST and TS was improved, as well as that of the male offspring of groups TT and ST. In addition, there was a discrete reduction of adiposity in the offspring when at least one of the parents was trained.Conclusion: Therefore, parental resistance training improved insulin sensitivity and adiposity of male and female offspring suggesting resistance training as a beneficial preconception health strategy for better metabolic outcomes in future generations.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-10"},"PeriodicalIF":2.5,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of apigenin, hesperidin and their combinations on different physiopathological pathways in 5-fluorouracil-induced pulmonary damage. 芹菜素、橙皮苷及其联用对5-氟尿嘧啶致肺损伤不同生理病理通路的影响。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-07-14 DOI: 10.1080/13813455.2025.2531443

Hamit Uslu, Gözde Atila Uslu, Taha Abdulkadir Çoban, Mustafa Özkaraca, Nezahat Kurt, Ali Sefa Mendil

{"title":"Effects of apigenin, hesperidin and their combinations on different physiopathological pathways in 5-fluorouracil-induced pulmonary damage.","authors":"Hamit Uslu, Gözde Atila Uslu, Taha Abdulkadir Çoban, Mustafa Özkaraca, Nezahat Kurt, Ali Sefa Mendil","doi":"10.1080/13813455.2025.2531443","DOIUrl":"https://doi.org/10.1080/13813455.2025.2531443","url":null,"abstract":"Background: Chemotherapeutics target cancerous cells, but they also have unavoidable toxicities in healthy tissues.Aim: In this study, the effects of the commonly used chemotherapeutic 5-fluorouracil (5FU) on lung tissue were investigated, along with the possible protective benefits of apigenin (API), hesperidin (HES), and their combination.Methodology: The study consisted of control, 5FU, API + 5FU, HES + 5FU, and API+HES + 5FU groups. API 50 mg/kg and HES 200 mg/kg were administered for 7 days. On the 8th day, 5FU was administered a dose of 100 mg/kg.Results: Analyses showed that API and HES were effective in preventing oxidative stress induced by 5FU in lung tissue, attenuating inflammation and apoptosis by suppressing MAPK/NFκB and Caspase-3/Bax/Bcl-2 pathways, suppressing autophagy by decreasing LC3B expression, and regulating Sigmar1 expression.Conclusion: These results suggest that the two flavonoids, when administered separately or in combination, may be useful in reducing side effects that often occur during the use of chemotherapeutics.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-15"},"PeriodicalIF":2.5,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144625217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An elevated level of one-hour postprandial plasma glucose is an independent risk factor for developing chronic complications of type 2 diabetes mellitus. 餐后1小时血糖升高是2型糖尿病慢性并发症的独立危险因素。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-07-11 DOI: 10.1080/13813455.2025.2529280

Yanyan Jin, Jing Wei, Jie Yang, Hongxia Qian, Yan Wu, Mengmeng Liu

{"title":"An elevated level of one-hour postprandial plasma glucose is an independent risk factor for developing chronic complications of type 2 diabetes mellitus.","authors":"Yanyan Jin, Jing Wei, Jie Yang, Hongxia Qian, Yan Wu, Mengmeng Liu","doi":"10.1080/13813455.2025.2529280","DOIUrl":"https://doi.org/10.1080/13813455.2025.2529280","url":null,"abstract":"Context: Elevated postprandial glucose levels can help identify individuals with normal glucose tolerance who are at high risk of developing type 2 diabetes and cardiovascular complications.Objective: This study aimed to investigate the correlation between 1-hour postprandial blood glucose (1hPG) and chronic complications of T2DM, including macrovascular and microvascular diseases.Materials and methods: A total of 194 patients diagnosed with T2DM were recruited and classified into complication and non-complication groups. On the basis of the 1hPG cut-off point of 8.6 mmol/L, these patients were divided into three groups (1hPG ≥ 11.1 mmol/L, 8.6 mmol/L ≤ 1hPG < 11.1 mmol/L and 1hPG < 8.6 mmol/L). The incidence of T2DM-related chronic complications was compared among the three groups. Receiver operating characteristic (ROC) curve and logistic regression analysis were used to evaluate the predictive capability of 1hPG for T2DM-related chronic complications.Results: A higher 1hPG level was found in patients affected by T2DM combined with chronic complications than those without complications. As 1hPG level decreased, the incidence rate of chronic complications in patients with T2DM decreased, which was indicative of a positive correlation between them. According to ROC analysis, FPG, 1hPG, and 2hPG could assist in predicting occurrence of T2DM-related chronic complications, while 1hPG had stronger predictive value. More importantly, logistic regression analysis further demonstrated that increased 1hPG level was an independent risk factor for chronic complications of T2DM.Discussion and conclusion: These findings indicate that an elevated 1hPG level may be associated with an increased risk of chronic complications of T2DM.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-9"},"PeriodicalIF":2.5,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The protective effects of thymoquinone against tartrazine-induced pancreatic injury and its impact on oxidative stress, caspase 3, blood glucose, insulin and cholesterol levels. 百里醌对酒石黄诱导的胰腺损伤的保护作用及其对氧化应激、半胱天冬酶3、血糖、胰岛素和胆固醇水平的影响。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-07-10 DOI: 10.1080/13813455.2025.2531121

Zeynep Erdemli, Emrah Zayman, Nurcan Gokturk, Mehmet Gul, Nursena Demircigil, Ayse Betul Levent, Bülent Aylaz, Harika Gozukara Bag, Mehmet Erman Erdemli

{"title":"The protective effects of thymoquinone against tartrazine-induced pancreatic injury and its impact on oxidative stress, caspase 3, blood glucose, insulin and cholesterol levels.","authors":"Zeynep Erdemli, Emrah Zayman, Nurcan Gokturk, Mehmet Gul, Nursena Demircigil, Ayse Betul Levent, Bülent Aylaz, Harika Gozukara Bag, Mehmet Erman Erdemli","doi":"10.1080/13813455.2025.2531121","DOIUrl":"https://doi.org/10.1080/13813455.2025.2531121","url":null,"abstract":"The present study examined the effects of Tartrazine, a common industrial food colourant, on the pancreas and the protective role of Thymoquinone. Thirty-two Wistar albino male rats were randomly divided into four equal groups: Control, Tartrazine, Thymoquinone, and Tartrazine + Thymoquinone. The rats received Tartrazine and Thymoquinone treatments for 21 days. At the end of this period, pancreatic tissues and blood samples were collected for analysis. Tartrazine administration elevated malondialdehyde (MDA), total oxidant status (TOS), and oxidative stress index (OSI) levels, while decreasing glutathione (GSH), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), and total antioxidant status (TAS) in pancreatic tissue. It increased glucose, total cholesterol, triglycerides, and LDL levels, while decreasing insulin and HDL levels in blood samples. Tartrazine administration aggravated pancreatic histopathology and enhanced Caspase-3 positive immunoreactivity. Thymoquinone administration reduced the harmful effects of Tartrazine on biochemical and histopathological parameters. Tartrazine administration negatively impacted pancreatic tissue and blood samples. The increased oxidant capacity and oxidative stress led to these harmful effects. Conversely, Thymoquinone alleviated oxidative stress by increasing antioxidant capacity and could act as a protective agent.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-11"},"PeriodicalIF":2.5,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ellagic acid protects against gastric ulcer in male rats by antioxidant and anti-inflammatory mechanisms: modulation of NF-κβ/COX-2 and Nrf2/HO-1 pathways. 鞣花酸通过调节NF-κβ/COX-2和Nrf2/HO-1通路的抗氧化和抗炎机制保护雄性大鼠的胃溃疡。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-07-07 DOI: 10.1080/13813455.2025.2529295

Mamdouh Eldesoqui, Emad A Albadawi, Amal F Dawood, Rania H M Soliman, Heba S Ahmed, Zeinab M Saeed, Nisreen E Elwany, Wesam M R Ashour, Mohamed Elshafey, Safa Mousa Al-Haider, Eman M A Abdelghany, Ahmed A El-Mansi, Sahar K Ali

{"title":"Ellagic acid protects against gastric ulcer in male rats by antioxidant and anti-inflammatory mechanisms: modulation of NF-κβ/COX-2 and Nrf2/HO-1 pathways.","authors":"Mamdouh Eldesoqui, Emad A Albadawi, Amal F Dawood, Rania H M Soliman, Heba S Ahmed, Zeinab M Saeed, Nisreen E Elwany, Wesam M R Ashour, Mohamed Elshafey, Safa Mousa Al-Haider, Eman M A Abdelghany, Ahmed A El-Mansi, Sahar K Ali","doi":"10.1080/13813455.2025.2529295","DOIUrl":"https://doi.org/10.1080/13813455.2025.2529295","url":null,"abstract":"Background: Gastric ulcer is commonly affected by several causes, including stress. This work examined the gastroprotective effects of ellagic acid (EA), in stress-induced gastric ulcers in Wistar rats.Methods: Forty male Wistar rats were categorized into five groups: a normal control group, a stress-induced ulcer group, and three groups receiving EA treatment (5 mg, 10 mg, and 20 mg). Gastric ulcers were elicited using a water immersion stress model. Macroscopic and histological assessments, together with biochemical immunohistochemical studies were performed.Results: EA therapy markedly decreased ulcer scores and indices in a dose-dependent manner. EA decreased TNF-α, IL-1β and MDA and augmented PGE1 and GSH. Histopathological assessments verified the results. The immunohistochemical analysis revealed increased Nrf2 and HO-1 levels and decreased NF-κB and COX-2 levels in the EA-treated groups.Conclusion: EA demonstrates gastroprotective properties against stress-induced gastric ulcers via its anti-inflammatory and antioxidant mechanisms in a dose-dependent manner.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-16"},"PeriodicalIF":2.5,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the mechanism of Coenzyme Q10 in ameliorating ageing related oxidative and inflammatory lung alterations in rats via targeting PI3K/AKT/Nrf-2 signaling pathway. 揭示辅酶Q10通过靶向PI3K/AKT/Nrf-2信号通路改善大鼠衰老相关的氧化和炎症性肺改变的机制。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-07-01 DOI: 10.1080/13813455.2025.2525415

Elshymaa A Abdel-Hakeem, Nisreen D M Toni, Doaa Mohamed Elroby Ali, Heba Marey, Heba A Abdel-Hamid

{"title":"Unveiling the mechanism of Coenzyme Q10 in ameliorating ageing related oxidative and inflammatory lung alterations in rats via targeting PI3K/AKT/Nrf-2 signaling pathway.","authors":"Elshymaa A Abdel-Hakeem, Nisreen D M Toni, Doaa Mohamed Elroby Ali, Heba Marey, Heba A Abdel-Hamid","doi":"10.1080/13813455.2025.2525415","DOIUrl":"https://doi.org/10.1080/13813455.2025.2525415","url":null,"abstract":"Objectives: Lung is one of the vital organs that is affected by the ageing process. Searching for natural antioxidants is mandatory to boost healthier longevity. Accordingly, we sought to explore the probable protective effect of Coenzyme Q10 (Q10) on experimentally induced lung ageing and study the supposed involved mechanistic pathways.Methods: Rats were allocated into groups; control, D-galactose (D-gal), D-gal + Q10 and D-gal + Q10+ LY294002 (LY; phosphatidylinositol 3-kinases (PI3K)/protein kinase B (AKT) inhibitor). Sera and lung tissues were gathered for evaluating markers of oxidative stress, inflammation, fibrosis, and cell senescence by different methods.Immunohistochemistry for senescence associated beta galactosidase (SA-βGal), Capsase-3, and P53 were also evaluated.Results: Induction of lung ageing resulted in deleterious lung alterations which were ameliorated by Q10; however, its protective effect was abolished by co-administration of LY.Conclusion: Q10 secured the lung against the ageing process via its antioxidant, anti-inflammatory, and anti-apoptotic effects through the activation of the PI3K/AKT/Nrf-2 pathway.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-17"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An efficient attention Densenet with LSTM for lung disease detection and classification using X-ray images supported by adaptive R2-Unet-based image segmentation. 基于自适应r2 - unet图像分割的基于LSTM的高效关注密度网络用于肺部疾病检测和分类。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-07-01 DOI: 10.1080/13813455.2025.2524182

Sashi Kanth Betha, Dondapati Rajendra Dev, Kalyani Sunkara, Pradeep Vinaik Kodavanti, Anusha Putta

{"title":"An efficient attention Densenet with LSTM for lung disease detection and classification using X-ray images supported by adaptive R2-Unet-based image segmentation.","authors":"Sashi Kanth Betha, Dondapati Rajendra Dev, Kalyani Sunkara, Pradeep Vinaik Kodavanti, Anusha Putta","doi":"10.1080/13813455.2025.2524182","DOIUrl":"https://doi.org/10.1080/13813455.2025.2524182","url":null,"abstract":"Lung diseases represent one of the most prevalent health challenges globally, necessitating accurate diagnosis to improve patient outcomes. This work presents a novel deep learning-aided lung disease classification framework comprising three key phases: image acquisition, segmentation, and classification. Initially, chest X-ray images are taken from standard datasets. The lung regions are segmented using an Adaptive Recurrent Residual U-Net (AR2-UNet), whose parameters are optimised using Enhanced Pufferfish Optimisation Algorithm (EPOA) to enhance segmentation accuracy. The segmented images are processed using \"Attention-based Densenet with Long Short Term Memory(ADNet-LSTM)\" for robust categorisation. Investigational results demonstrate that the proposed model achieves the highest classification accuracy of 93.92%, significantly outperforming several baseline models including ResNet with 90.77%, Inception with 89.55%, DenseNet with 89.66%, and \"Long Short Term Memory (LSTM)\" with 91.79%. Thus, the proposed framework offers a dependable and efficient solution for lung disease detection, supporting clinicians in early and accurate diagnosis.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-31"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insulin- and glucagon-producing cells in the liver and biliary-pancreatic axis of rats with experimentally induced metabolic syndrome. 实验性代谢综合征大鼠肝脏和胆胰轴中胰岛素和胰高血糖素生成细胞的研究。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-06-28 DOI: 10.1080/13813455.2025.2503482

M Gulubova, A Tolekova, D Berbatov, I Stefanov, D Chonov, N Aydoglu

{"title":"Insulin- and glucagon-producing cells in the liver and biliary-pancreatic axis of rats with experimentally induced metabolic syndrome.","authors":"M Gulubova, A Tolekova, D Berbatov, I Stefanov, D Chonov, N Aydoglu","doi":"10.1080/13813455.2025.2503482","DOIUrl":"https://doi.org/10.1080/13813455.2025.2503482","url":null,"abstract":"Context: The generation of insulin-producing cells (IPCs) as cell replacement therapy for diabetes treatment is challenging.Objective: We have evaluated the presence of insulin-positive (insulin+) and glucagon-positive (glucagon+) cells in hepatocytes, peribiliary glands (PBGs), and liver sinusoidal endothelial cells (LSECs).Materials and methods: Wistar rats are subjected to a diet including administration of 15% fructose solution for 3 months. Tissue samples are processed for immunohistochemistry with antibodies against insulin, glucagon, ghrelin, somatostatin, PDX1, and SOX9. Blood glucose levels and lipid profile are investigated.Results: In treated rats, Ins+ and glucagon+ hepatocytes are found around central veins. In PBGs, Ins+ and glucagon+ endocrine cells (ECs) are detected. LSECs show insulin+ and glucagon+ cellular membranes. The nuclei of LSECs in treated rats are SOX9-positive.Conclusions: Our experiment of fructose-induced metabolic syndrome shows the appearance of Ins+ and glucagon+ ECs in extrahepatic biliary pathways and hepatocytes. Interestingly, SOX9+ nuclei of LSECs are observed.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-9"},"PeriodicalIF":2.5,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0