Archives of Physiology and Biochemistry最新文献

{"title":"Effects of herbaceous bioflavonoid herbacetin on oxidative stress, and alpha-synuclein regulation, programmed cell death in a Parkinson illness.","authors":"Chinnadurai Veeramani, Mohammed A Alsaif, Muhammad Ibrar Khan, Ahmed S El Newehy, Ali Alshammari, Khalid S Al-Numair","doi":"10.1080/13813455.2025.2493103","DOIUrl":"https://doi.org/10.1080/13813455.2025.2493103","url":null,"abstract":"<p><strong>Background: </strong>Herbacetin, a flavonoid present in many types of herbs, which include linaceae, ephedraceae, and crassulaceae, exhibits a range of medicinal properties. 1-methyl-4-phenylpyridinium (MPP⁺) is one of the neurotoxins used in cell-based Parkinson's disease (PI) models. Whereas the precise chemical mechanism of iron association with free radical cell damage and apoptosis is yet unknown, intracellular irons are a key factor for MPP⁺-derived apoptosis.</p><p><strong>Methods: </strong>We examine whether the antiapoptotic properties of flaxseed bioflavonoid herbacetin (HB) are associated with the stimulation of the intrinsic caspase-dependent pathway and exposing of MPP⁺ caused neuronal death in the human dopaminergic neuroblastoma cells. Four groups were created out of the cells. Groups I, II, III, and IV are the control, HB+MPP⁺, MPP⁺, and HB, respectively. Following a 24-hour incubation period, the cells were subjected to several parameters.</p><p><strong>Results: </strong>We discovered in neuroblastoma cells that HB dramatically reduced the cell death induced by MPP⁺. Additionally, HB significantly reduced the formation of ROS and counteracted the reduction in MMP resulting from MPP⁺ treatment. HB reduces the stimulation of the intrinsic caspase-dependent apoptotic mechanism and suppresses the MPP⁺-mediated apoptotic signalling pathway. Furthermore, HB predicted a better binding interaction with alpha-synuclein and drastically decreased alpha-synuclein expression and accumulation in neuroblastoma cells.</p><p><strong>Conclusion: </strong>Consequently, our findings imply that HB shields neurons by reducing oxidative stress, alpha-synuclein misfolding in neuroblastoma, and apoptosis prompts the death of neuroblastoma cells.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-12"},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between MTHFR 677C > T polymorphism and serum PIVKA-II levels in hepatocellular carcinoma.","authors":"Hongyu Zhang, Baixiu Wu, Liang Zhang, Zheng Peng","doi":"10.1080/13813455.2025.2493107","DOIUrl":"https://doi.org/10.1080/13813455.2025.2493107","url":null,"abstract":"<p><strong>Background: </strong>Hepatocellular carcinoma (HCC) is a major public health problem with increasing incidence and mortality worldwide. The methylenetetrahydrofolate reductase (MTHFR) 677 C > T polymorphism is associated with the development and progression of various tumours, while protein induced by vitamin K absence II (PIVKA-II) is an important tumour marker for the diagnosis of HCC. This study aims to investigate the relationship between the MTHFR 677 C > T polymorphism and serum PIVKA-II levels in HCC patients, providing new insights for early diagnosis, risk assessment, and prognosis evaluation of HCC.</p><p><strong>Methods: </strong>This study included 120 HCC patients and 100 healthy controls. MTHFR 677 C > T genotyping was performed using fluorescent quantitative PCR, and serum PIVKA-II levels were measured. Bioinformatics analysis was used to explore the expression of the MTHFR gene in HCC and its relationship with prognosis.</p><p><strong>Results: </strong>MTHFR 677 C > T TT carriers had an increased risk of HCC (OR = 2.393; 95% CI 1.055-5.429; <i>p</i> = 0.037); the risk of HCC for T gene carriers was 58.3% higher than that for C gene carriers in the allele model (OR = 1.583; 95% CI 1.059-2.364; <i>p</i> = 0.025). The difference in serum PIVKA-II concentration was statistically significant between the controls, stage I-II patients, and stage III-IV patients (<i>p</i> < 0.05), and the difference in serum PIVKA-II concentration was statistically significant between patients with the TT genotype and patients with the CC and CT genotypes (all <i>p</i> values less than 0.05). UALCAN database analysis showed that MTHFR gene expression levels were increased in patients with HCC, and the high expression of the MTHFR gene was negatively correlated with patient survival rates.</p><p><strong>Conclusions: </strong>There is an association between the MTHFR 677 C > T TT genotype and serum PIVKA-II levels in HCC. This could help identify high-risk individuals and assess disease severity, providing a potential genetic biomarker for the diagnosis of HCC.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-8"},"PeriodicalIF":2.5,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum levels of omentin and visfatin in patients with metabolic syndrome.","authors":"Anita Sherly A, Rukmini M S, Anupama Hegde, Arun S, Himani Kotian","doi":"10.1080/13813455.2025.2486290","DOIUrl":"https://doi.org/10.1080/13813455.2025.2486290","url":null,"abstract":"<p><strong>Introduction: </strong>Metabolic Syndrome (MetS) is a global health concern characterised by cardiometabolic risk factors, dysregulated adipokine signalling and inflammation. The study aimed to assess the serum levels of omentin and visfatin in patients with metabolic syndrome.</p><p><strong>Methods: </strong>The 84-subject hospital-based case-control study included 18-55 years, both genders. Anthropometry, medical history, fasting plasma glucose (FPG), lipid profile, and HOMA-IR were collected. Insulin, omentin, and visfatin were measured using ELISA.</p><p><strong>Results: </strong>Omentin and visfatin levels significantly differed between groups (<i>p</i> < 0.05). The median omentin levels were 50.74 and 45.25; visfatin levels were 0.064 and 0.001, respectively. Omentin correlated with waist circumference, blood pressure, and FPG in controls, while visfatin correlated with HDL and BMI among cases (<i>p</i> < 0.05). Omentin and visfatin were elevated in cases. However, no significant correlation between omentin and visfatin with lipid parameters could be established.</p><p><strong>Conclusion: </strong>Omentin and visfatin levels varied significantly between metabolic syndrome and controls; their correlation with MetS criteria was not significant.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-8"},"PeriodicalIF":2.5,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of elevated levels of the gut metabolite TMAO on glucose metabolism after sleeve gastrectomy.","authors":"Zhiping Huang, Chaoqian Liu, Xiang Zhao, Yan Guo","doi":"10.1080/13813455.2025.2489721","DOIUrl":"10.1080/13813455.2025.2489721","url":null,"abstract":"<p><p><b>Purpose：</b>Bariatric surgery can effectively alleviate obesity and diabetes by regulation of the gut microbiota. This study aimed to investigate the change in the gut microbiota metabolite TMAO and to explore its effect on glucose metabolism after sleeve gastrectomy (SG).</p><p><p><b>Materials and methods：</b>Diet-induced obesity mouse models were established, and the mice were randomly divided into four groups: an SG group, a sham-operated group pair-fed with the SG group (PF), a sham-operated group fed ad libitum (AL), and a lean control group (C). At 10 weeks post-surgery, the changes in glycogen content of liver, gut microbiota and the level of FMO3 in the liver were evaluated, and their correlation with TMAO production was analysed. The expression levels of the TMAO/PERK/FOXO1 pathway and the gluconeogenic genes G6PC and PCK1 were measured.</p><p><p><b>Results：</b>At 10 weeks post-surgery, hepatocyte glycogen levels were restored, and serum TMA and TMAO levels were significantly increased. Faecal metagenomic sequencing results showed that the abundances of Ruminococcaceae and Lachnospiraceae, which were positively correlated with TMAO production, were significantly increased after surgery. While the changes in FMO3, the key enzyme producing TMAO in the liver was found decreased significantly after SG. The expression levels of the TMAO/PERK/FOXO1 pathway and the gluconeogenic genes G6PC and PCK1 were measured. Inconsistent with the changing trend of TMAO, the expression of PERK, FOXO1, PCK, and G6PC significantly decreased after SG.</p><p><p><b>Conclusions：</b>SG can significantly reduce obesity and restore glucose metabolism. After surgery, TMAO metabolites increased in a microbiota-dependent manner.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-10"},"PeriodicalIF":2.5,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin D₃ administration ameliorates the anxiety and depressive-like behaviour induced by nicotine withdrawal: a mechanistic focus on oxidative stress, inflammatory response, and serotonergic transmission.","authors":"Farimah Beheshti, Mehrnoush Goudarzi, Samaneh Kakhki, S Mohammad Ahmadi-Soleimani, Mustafa Ansari, Hassan Azhdari-Zarmehri","doi":"10.1080/13813455.2025.2483508","DOIUrl":"https://doi.org/10.1080/13813455.2025.2483508","url":null,"abstract":"<p><strong>Background: </strong>The present study conducted to assess whether vitamin D₃ (Vit D) could ameliorate the anxiety and depression induced by nicotine (Nic) withdrawal in male adult rats.</p><p><strong>Methods: </strong>To this end, behavioural tests were done in male Wistar rats undergone adolescent Nic exposure (2 mg/kg) and then withdrawal and the effect of Vit D (100, 1000, and 10,000 IU/kg) was assessed at both behavioural and biochemical levels.</p><p><strong>Results: </strong>Results indicated that Vit D treatment could effectively prevent anxiety, depression, and biochemical alterations induced by Nic withdrawal.</p><p><strong>Conclusion: </strong>Vit D has strong potential to be used for prevention of anxiety- and depressive-like behaviours following Nic withdrawal; however, further investigation is needed in larger sample size to discuss more confidently.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-10"},"PeriodicalIF":2.5,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GC-MS analysis, <i>the in vitro</i> and <i>in vivo</i> protective effect of <i>Phlogacanthus thyrsiflorus</i> Nees. on hyperglycaemia-induced diabetic mice model.","authors":"Jutishna Bora, Sumira Malik, Richa Mishra, Sarvesh Rustagi, Petr Slama, Smita Lata, Nayan Talukdar, Saad Alghamdi, Abdullah Aldairi, Faten Noor Habiballah, Mazen Almehmadi, Osama Abdulaziz, Naif Alsiwiehri, Seema Ramniwas","doi":"10.1080/13813455.2025.2483501","DOIUrl":"https://doi.org/10.1080/13813455.2025.2483501","url":null,"abstract":"<p><p><b>Objective:</b> This study investigates the in-vitro and in-vivo antioxidant capacities showed by <i>Phlogacanthus thyrsiflorus</i> Nees. (<i>P. thyrsiflorus</i>) in alloxan-administered diabetic mice.</p><p><p><b>Materials and Methods:</b> The screening of phytochemical of methanolic flower extract (MFE), Gas Chromatography Mass Spectrometry (GC-MS) profiling was utilized to identify bioactive compounds. In-vitro antioxidant studies were performed. Acute toxicity was evaluated in mice. Glucose Transporter type 4 (GLUT4) protein expression and antioxidant enzyme activities were assessed. Histopathological examination of heart tissue was performed.</p><p><p><b>Results:</b> GC-MS analysis revealed the presence of various bioactive compounds consisting of antioxidant, anti-inflammatory activities. The results also showed a noteworthy increase in in-vitro and in-vivo antioxidant enzymes activities. Moreover, MFE suppress hyperglycaemia by upregulating GLUT4 protein expression. In histological study MFE was found to restore cellular alterations in diabetic tissue.</p><p><p><b>Discussion and Conclusion:</b> It is inferred from the study that MFE of <i>P. thyrsiflorus</i> can exert a protective effect by suppressing hyperglycaemia and modulating oxidative stress in alloxan-administered diabetic mice.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-12"},"PeriodicalIF":2.5,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination therapy of systemic and local metformin improves imiquimod-induced psoriasis-like lesions with type 2 diabetes: the role of AMPK/KGF/STAT3 axis.","authors":"Fatma ElSayed Hassan, Basma Emad Aboulhoda, Marwa Nagi Mehesen, Passant Mohie El Din, Hend Ahmed Abdallah, Ehab R Bendas, Laila Ahmed Rashed, Abeer Mostafa, Marwa Fathy Amer, Marwa Abdel-Rahman, Mansour A Alghamdi, Asmaa Mohammed Shams Eldeen","doi":"10.1080/13813455.2024.2407547","DOIUrl":"10.1080/13813455.2024.2407547","url":null,"abstract":"<p><strong>Context: </strong>Insulin resistance and a disturbed lipid profile are common associations with type 2 diabetes mellitus (T2DM) and different skin diseases, particularly psoriasis (PsO).</p><p><strong>Objectives: </strong>We investigated potential therapeutic mechanisms of metformin in a murine animal model of psoriasiform lesions in T2DM.</p><p><strong>Materials and methods: </strong>Forty-two rats were randomly divided into control, PsO, and type II DM (T2DM) groups. After confirmation of DM, the type II diabetic rats were allocated into T2DM+ PsO, T2DM+ PsO+ systemic metformin (S. met), T2DM+ PsO+ topical metformin (T. met)), and T2DM+ PsO + combined metformin (C. met). PsO was induced by topical imiquimod.</p><p><strong>Results: </strong>Systemic administration of the cornerstone antidiabetic drug, metformin, was able to improve insulin resistance and lipid profile. At molecular levels, both topical and systemic metformin significantly increased AMP-activated protein kinase (AMPK), and lowered keratinocyte growth factor (KGF) / \"Signal transducer and activator of transcription\" (STAT)3 protein levels, and the IL-17RA and IL-17RC gene expression.</p><p><strong>Conclusion: </strong>Although its glucose-controlling effect was not optimum, T.met gel served anti-psoriatic and anti-inflammatory effects.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"252-264"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of <i>Helicobacter pylori</i>-derived extracellular vesicles on glucose metabolism and induction of insulin resistance in HepG2 cells.","authors":"Ghazaleh Talebi, Parvaneh Saffarian, Mojdeh Hakemi-Vala, Amir Sadeghi, Abbas Yadegar","doi":"10.1080/13813455.2024.2418494","DOIUrl":"10.1080/13813455.2024.2418494","url":null,"abstract":"<p><p><i>Helicobacter pylori</i> infection has been associated with the development of insulin resistance (IR). This study aimed to examine the effect of <i>H. pylori</i>-derived extracellular vesicles (EVs) on IR induction. EVs were derived from two <i>H. pylori</i> strains, and characterised by transmission electron microscopy and dynamic light scattering. Different concentrations of insulin were added to HepG2 cells to induce IR model. HepG2 cells were exposed to various concentrations of <i>H. pylori</i>-derived EVs to assess IR development. The gene expression of <i>IRS1</i>, <i>AKT2</i>, <i>GLUT2</i>, <i>IL-6</i>, <i>SOCS3</i>, <i>c-Jun</i> and miR-140 was examined using RT-qPCR. Glucose uptake analysis revealed insulin at 5 × 10 ^-7mol/l and EVs at 50 µg/ml induced IR model in HepG2 cells. <i>H. pylori</i>-derived EVs downregulated the expression level of <i>IRS1</i>, <i>AKT2</i>, and <i>GLUT2</i>, and upregulated <i>IL-6</i>, <i>SOCS3</i>, <i>c-Jun</i>, and miR-140 expression in HepG2 cells. In conclusion, our findings propose a novel mechanism by which <i>H. pylori-</i>derived EVs could potentially induce IR.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"316-327"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of exogenous ghrelin treatment on oxidative stress, inflammation and histological parameters in a fat-fed streptozotocin rat model.","authors":"Ozlem Ergul Erkec, Zubeyir Huyut, Eda Acikgoz, Mehmet Tahir Huyut","doi":"10.1080/13813455.2024.2407551","DOIUrl":"10.1080/13813455.2024.2407551","url":null,"abstract":"<p><p>In this study, the anti-inflammatory, antioxidative, and protective effects of ghrelin were investigated in a fat-fed streptozotocin (STZ) rat model and compared with metformin, diabetes and the healthy control groups. Histopathological evaluations were performed on H&E-stained pancreas and brain sections. Biochemical parameters were investigated by enzyme-linked immunosorbent assay. Blood glucose levels were significantly decreased with ghrelin or metformin treatments than the diabetes group. STZ administration increased brain, renal and pancreatic IL-1β, TNF-α and MDA while decreasing GPX, CAT, SOD, and NGF levels. Ghrelin increased renal GPX, CAT, NGF pancreatic GPX, SOD, CAT, NGF and brain SOD, NGF while it decreased renal, pancreatic and brain IL-1β, TNF-α and MDA levels. Ghrelin reduced neuronal loss and degeneration in the cerebral cortex and hippocampus and greatly ameliorated diabetes-related damage in pancreas. In conclusion, the data suggested that ghrelin is an effective candidate as a protectant for reducing the adverse effects of diabetes.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"274-284"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction.","authors":"","doi":"10.1080/13813455.2024.2418702","DOIUrl":"10.1080/13813455.2024.2418702","url":null,"abstract":"","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"328"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}