Archives of Physiology and Biochemistry最新文献_第3页

Evaluation of the clinical significance of BTG1 gene expression and pepsinogen in serum and cancerous tissue and gastric atrophy. BTG1基因表达及胃蛋白酶原在血清、癌组织及胃萎缩中的临床意义

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-06-01 Epub Date: 2025-02-23 DOI: 10.1080/13813455.2025.2458560

Yousef Paridar, Homa Hosseinpour, Maysam Mard-Soltani, Somayeh Pouria Mehr, Neda Shakerian, Davood Alinezhad Dezfuli, Saeed Khalili, Mohammad Reza Abyaz

{"title":"Evaluation of the clinical significance of BTG1 gene expression and pepsinogen in serum and cancerous tissue and gastric atrophy.","authors":"Yousef Paridar, Homa Hosseinpour, Maysam Mard-Soltani, Somayeh Pouria Mehr, Neda Shakerian, Davood Alinezhad Dezfuli, Saeed Khalili, Mohammad Reza Abyaz","doi":"10.1080/13813455.2025.2458560","DOIUrl":"10.1080/13813455.2025.2458560","url":null,"abstract":"Introduction: This study aimed to assess the expression changes of BTG1, PGI, and PGII in tissues and serum of patients with gastric cancer, atrophic gastritis, and healthy individuals.Methods: QRT-PCR was used to measure BTG1, PGI, and PGII expression in 30 cancers, 30 atrophic gastritis, and 30 healthy tissue samples. Serum levels of PGI and PGII were measured using ELISA. Statistical tests included the Mann-Whitney U and independent T-test. Covariates like tumour stage and H. pylori status were considered.Results: BTG1 expression was significantly lower in cancer and gastritis tissues. Serum PGI and PGII levels were significantly reduced in cancer patients (P ≤ 0.001).Discussion: The PGI/PGII ratio in serum emerged as a strong non-invasive biomarker for distinguishing cancer from healthy individuals. While BTG1 provides insights into gastric carcinogenesis, its clinical utility is limited due to the need for tissue samples. The serum-based PGI/PGII ratio shows greater promise as a non-invasive screening tool for GC.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"503-512"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143481749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melatonin improves adverse vascular remodelling and redox homeostasis in monocrotaline-induced pulmonary arterial hypertension. 褪黑素可改善单芥碱诱导的肺动脉高压的不良血管重构和氧化还原稳态。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-06-01 Epub Date: 2025-01-02 DOI: 10.1080/13813455.2024.2446822

Silvio Tasca, Patrick Türck, Daniela Drosdowski, Cristina Campos Carraro, Adriane Belló-Klein, Alexandre Luz De Castro, Alex Sander Da Rosa Araujo

{"title":"Melatonin improves adverse vascular remodelling and redox homeostasis in monocrotaline-induced pulmonary arterial hypertension.","authors":"Silvio Tasca, Patrick Türck, Daniela Drosdowski, Cristina Campos Carraro, Adriane Belló-Klein, Alexandre Luz De Castro, Alex Sander Da Rosa Araujo","doi":"10.1080/13813455.2024.2446822","DOIUrl":"10.1080/13813455.2024.2446822","url":null,"abstract":"This study explored the effects of melatonin on cardiac and vascular function, and redox homeostasis in model PAH. Male Wistar rats were divided into: control (CTR), monocrotaline [MCT (60 mg/kg, single dose i.p)], monocrotaline + sildenafil [MCT + SIL (50 mg/kg/day)], and monocrotaline + melatonin [MCT + MEL (10 mg/kg/day)]. This protocol lasted 21 days. Echocardiographic, morphometric, histological, vascular reactivity, and oxidative/nitrosative stress analyses were performed. The reduced diastolic function and AT/ET ratio in the MCT group were partially attenuated by melatonin and sildenafil treatment (p < 0.05). Increased RV hypertrophy and pulmonary congestion were reduced by both treatments (p < 0.05). MCT-induced pulmonary arteriolar muscle layer hypertrophy was also reduced by both treatments (p < 0.05). MCT and MCT + SIL present diminished vasorelaxation as compared to control (p < 0.05). Augmented oxidative/nitrosative stress and reduced glutathione-s-transferase activity in MCT were mitigated by both treatments (p < 0.05). Then, melatonin was as effective as sildenafil against PAH-induced oxidative stress and pathological vascular remodelling.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"455-466"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduced lipid and glucose oxidation and reduced lipid synthesis in AMPKα2^-/- myotubes. 减少AMPKα2-/-肌管中的脂质和葡萄糖氧化和脂质合成。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-06-01 Epub Date: 2025-01-09 DOI: 10.1080/13813455.2024.2449409

Christine Skagen, Stanislava Stevanovic, Hege Gilbø Bakke, Tuula A Nyman, Maria Stensland, Eili Tranheim Kase, Olga Horakova, Arild C Rustan, G Hege Thoresen

{"title":"Reduced lipid and glucose oxidation and reduced lipid synthesis in AMPKα2-/- myotubes.","authors":"Christine Skagen, Stanislava Stevanovic, Hege Gilbø Bakke, Tuula A Nyman, Maria Stensland, Eili Tranheim Kase, Olga Horakova, Arild C Rustan, G Hege Thoresen","doi":"10.1080/13813455.2024.2449409","DOIUrl":"10.1080/13813455.2024.2449409","url":null,"abstract":"Adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) plays a crucial role in regulation of metabolic homeostasis. To understand the role of the catalytic α2 subunit of AMPK in skeletal muscle energy metabolism, myotube cultures were established from AMPKα2+/+ and AMPKα2-/- mice. Myotubes from AMPKα2-/- mice had lower basal oleic acid and glucose oxidation compared to myotubes from AMPKα2+/+ mice. However, the relative response to mitochondrial uncoupling was increased for oleic acid oxidation. Incorporation of acetate into lipids was also lower in myotubes from AMPKα2-/- mice. Proteomics analysis revealed that AMPKα2-/- myotubes had upregulated pathways related to mitochondrial function and fatty acid oxidation, and decreased pathways related to fatty acid biosynthesis. In conclusion, ablation of AMPKα2 catalytic subunit in skeletal muscle cells resulted in reduced basal oxidation of glucose and fatty acids, however upregulated pathways related to mitochondrial function and fatty acid oxidation and reduced lipid formation.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"483-492"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tumour protein p53-activated lncRNA PGM5-AS1 suppresses lung cancer growth and stemness by targeting R-spondin1 via microRNA-1247-5p. 肿瘤蛋白p53激活的lncRNA PGM5-AS1通过microRNA-1247-5p靶向R-spondin1抑制肺癌的生长和干细胞。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-06-01 Epub Date: 2025-03-04 DOI: 10.1080/13813455.2025.2459318

Peng Yang, Hong Gu, Xuanqin Wu, Geng Chen, Heng Liu, Zhongliang Chen

{"title":"Tumour protein p53-activated lncRNA PGM5-AS1 suppresses lung cancer growth and stemness by targeting R-spondin1 via microRNA-1247-5p.","authors":"Peng Yang, Hong Gu, Xuanqin Wu, Geng Chen, Heng Liu, Zhongliang Chen","doi":"10.1080/13813455.2025.2459318","DOIUrl":"10.1080/13813455.2025.2459318","url":null,"abstract":"Objective: This study was to investigated the inhibitory role of the tumour protein p53 (TP53)-activated PGM5-AS1 in lung cancer (LC) cell proliferation, invasion, and CSC-like properties and its underlying mechanisms.Methods: The effect of PGM5-AS1 on LC cell development was determined. Stem cell markers, aldehyde dehydrogenase activity in cells were tested, as well as the ability of stem cells to form spheroids. The interaction of PGM5-AS1 and TP53 was determined. The binding link of PGM5-AS1, miR-1247-5p, and R-spondin1 (RSPO1) was verified.Results: PGM5-AS1 was elevated by a combination of TP53 and PGM5-AS1 promoters. PGM5-AS1 was a molecular sponge of miR-1247-5p in LC cells, and miR-1247-5p targeted RSPO1. Elevating PGM5-AS1 or repressing miR-1247-5p restrained LC cell growth and stemness, which were reversed by depression of RSPO1.Conclusion: This study conveys that TP53-elevated PGM5-AS1 mediates miR-1247-5p to target RSPO1, thereby inhibiting LC growth and stemness, representing a novel avenue for LC therapy.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"513-525"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AMPK activation; a potential strategy to mitigate TKI-induced cardiovascular toxicity. 激活 AMPK；减轻 TKI 诱导的心血管毒性的潜在策略。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-06-01 Epub Date: 2024-11-11 DOI: 10.1080/13813455.2024.2426494

Nasser Safaie, Gholamreza Idari, Diba Ghasemi, Mobasher Hajiabbasi, Vahid Alivirdiloo, Shahab Masoumi, Mahdi Zavvar, Ziba Majidi, Yousef Faridvand

{"title":"AMPK activation; a potential strategy to mitigate TKI-induced cardiovascular toxicity.","authors":"Nasser Safaie, Gholamreza Idari, Diba Ghasemi, Mobasher Hajiabbasi, Vahid Alivirdiloo, Shahab Masoumi, Mahdi Zavvar, Ziba Majidi, Yousef Faridvand","doi":"10.1080/13813455.2024.2426494","DOIUrl":"10.1080/13813455.2024.2426494","url":null,"abstract":"The introduction of Tyrosine Kinase Inhibitors (TKIs) has revolutionised cancer treatment, yet concerns regarding cardiovascular toxicity have surfaced. This piece delves into the interplay between AMP-activated protein kinase (AMPK) signalling and TKI-induced cardiovascular toxicity. The study unravels the intricate relationship between AMPK activation and TKI-induced cardiovascular toxicity, aiming to ascertain whether AMPK can play a strategic role in mitigating adverse effects. Beyond unravelling mechanistic insights, the research sets the stage for future therapeutic approaches, envisioning AMPK activation as a pivotal connection for balancing effective cancer treatment with cardiovascular well-being. As research advances, the potential of AMPK activation not only addresses challenges in TKI-induced cardiovascular toxicity but also shapes the future landscape of personalised anticancer therapies. The article explores the mechanisms of TKI-induced toxicity, AMPK's impact on cardiovascular health, and the potential therapeutic implications of AMPK activation in alleviating TKI-associated toxicities.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"329-341"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipophagy: exploring its association with male reproductive system disorders and investigating potential mechanisms. 脂肪摄食：探讨其与男性生殖系统障碍的关系及其潜在机制。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-06-01 Epub Date: 2025-01-08 DOI: 10.1080/13813455.2024.2446840

Wanyi Chen, Jin Chen, Ziqiong Cheng, Weilun Chen, Huiping Zhang

{"title":"Lipophagy: exploring its association with male reproductive system disorders and investigating potential mechanisms.","authors":"Wanyi Chen, Jin Chen, Ziqiong Cheng, Weilun Chen, Huiping Zhang","doi":"10.1080/13813455.2024.2446840","DOIUrl":"10.1080/13813455.2024.2446840","url":null,"abstract":"Background: Lipid metabolism, one of the three major metabolic processes, plays a crucial role in male fertility, particularly when lipid homeostasis is disrupted. Lipid droplets (LDs), cellular organelles that store lipids primarily in the form of triglycerides and cholesterol esters, serve as central hubs in lipid metabolism.The degradation of LDs is regulated by lipases and lipophagy.Objective:: This review explores the various forms of lipophagy, its molecular mechanisms, and its critical role in male fertility. Specifically, it examines the association between lipophagy and male infertility, sexual dysfunction, and reproductive cancers.Methods:: This review synthesizes current research on the molecular pathways regulating lipophagy, focusing on its impact on male reproductive health.Results:: Lipophagy is essential for maintaining lipid homeostasis in male reproductive tissues. Dysfunction of lipophagy is associated with impaired sperm function, infertility, sexual dysfunction, and an increased risk of reproductive cancers in men.Conclusion:: Lipophagy plays a pivotal role in regulating lipid metabolism and maintaining male fertility. It may serve as a potential therapeutic target for treating male reproductive disorders.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"366-378"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lepidine potentiates the antidiabetic effect of metformin in nicotinamide/streptozotocin-induced diabetic male rats. Lepidine增强二甲双胍对烟酰胺/链脲佐菌素诱导的糖尿病雄性大鼠的降糖作用。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-06-01 Epub Date: 2025-02-13 DOI: 10.1080/13813455.2025.2459865

Ayman S Soliman, Amira A Abdelfattah, Osama M Ahmed, Safy S Gaber

{"title":"Lepidine potentiates the antidiabetic effect of metformin in nicotinamide/streptozotocin-induced diabetic male rats.","authors":"Ayman S Soliman, Amira A Abdelfattah, Osama M Ahmed, Safy S Gaber","doi":"10.1080/13813455.2025.2459865","DOIUrl":"10.1080/13813455.2025.2459865","url":null,"abstract":"Metformin is used as a first-line treatment of type 2 diabetes mellitus (T2DM) and is effective as monotherapy and in combination with other glucose-lowering medications. Lepidine, a natural product found in Lepidium sativum, had antidiabetic, antioxidant, anticancer, anti-inflammatory, and hypolipidemic effects. This study aimed to assess the effects of lepidine alone and in combination with metformin in nicotinamide (NA)/streptozotocin (STZ)-induced diabetic Wistar rats. T2DM was induced by intraperitoneal injection of NA 15 minutes before intraperitoneal injection of STZ. Diabetic rats were orally treated with lepidine (20 mg/kg body weight) and/or metformin (70 mg/kg body weight) every other day for four weeks. Both lepidine and metformin treated diabetic rats showed a significant decrease in fasting blood glucose (FBG), amelioration of serum lipid profile, increase in serum insulin, and C-peptide , and downregulation in the elevated serum tumour necrosis α (TNF-α) level in association with the improvement in the pancreatic islet integrity and function. In conclusion, the lepidine has potent anti-diabetic effects and may add more to the therapeutic value of metformin when the two agents are used in combination. However, further clinical studies on human beings with T2DM are required to assess the efficacy and safety of the combination of metformin and lepidine before its approval and therapeutic application.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"526-538"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metformin attenuates inflammation and improves insulin sensitivity in coculture of LPS-induced 3T3-L1 adipocytes and RAW 264.7 macrophages mediated by IRS-1/GLUT-4 pathway. 二甲双胍可减轻lps诱导的3T3-L1脂肪细胞和IRS-1/GLUT-4途径介导的RAW 264.7巨噬细胞的炎症反应，提高胰岛素敏感性。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-06-01 Epub Date: 2025-02-03 DOI: 10.1080/13813455.2025.2460102

Siska Andrina Kusumastuti, Dwi Aris Agung Nugrahaningsih, Mae Sri Hartati Wahyuningsih

{"title":"Metformin attenuates inflammation and improves insulin sensitivity in coculture of LPS-induced 3T3-L1 adipocytes and RAW 264.7 macrophages mediated by IRS-1/GLUT-4 pathway.","authors":"Siska Andrina Kusumastuti, Dwi Aris Agung Nugrahaningsih, Mae Sri Hartati Wahyuningsih","doi":"10.1080/13813455.2025.2460102","DOIUrl":"10.1080/13813455.2025.2460102","url":null,"abstract":"Objective: Metformin is an anti-diabetic drug used to control blood glucose levels. The effects of metformin on insulin sensitivity in inflammation-induced adipocytes are not fully understood.This study aimed to explore the mechanism of metformin on insulin sensitivity enhancement in the coculture of LPS-induced 3T3-L1 adipocytes and RAW 264.7 macrophages.Material and methods: Insulin resistance was induced in coculture cells using Lipopolysaccharide, followed by adding 25, 50, and 100 µg/ml of metformin for 24 h of incubation. Glucose consumption, GLUT-4, IRS-1, and IL-6 mRNA expressions were quantified.Results: Metformin, starting at a concentration of 25 µg/ml, enhanced glucose consumption, upregulated GLUT-4 mRNA expression, and stimulated the expression of IRS-1 mRNA in coculture cells at 100 µg/ml of concentration. Additionally, Metformin inhibited inflammation by reducing IL-6 mRNA expression in coculture cells up to 100 µg/ml.Discussion and conclusion: These findings suggest that metformin attenuated inflammation and improved insulin sensitivity in inflammation-induced adipocytes that may be mediated by the IRS-1/GLUT-4 pathway.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"549-555"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beclin1/LC3II/P62 autophagy pathway activation is involved in the protective action of C-peptide against prostate injury in a rat model of type 1 diabetes. 在 1 型糖尿病大鼠模型中，Beclin1/LC3II/P62 自噬途径的激活参与了 C 肽对前列腺损伤的保护作用。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-06-01 Epub Date: 2024-11-04 DOI: 10.1080/13813455.2024.2422317

Heba A Abdel-Hamid, Manar Fouli Gaber Ibrahim, Doaa Mohamed Elroby Ali, Elshymaa A Abdel-Hakeem

{"title":"Beclin1/LC3II/P62 autophagy pathway activation is involved in the protective action of C-peptide against prostate injury in a rat model of type 1 diabetes.","authors":"Heba A Abdel-Hamid, Manar Fouli Gaber Ibrahim, Doaa Mohamed Elroby Ali, Elshymaa A Abdel-Hakeem","doi":"10.1080/13813455.2024.2422317","DOIUrl":"10.1080/13813455.2024.2422317","url":null,"abstract":"One of the undesirable complications of diabetes is sexual dysfunctions in males which may affect their fertility. This research aims to study the effect of C-peptide administration on the prostate of diabetic rats and focusing on exploring the role of the autophagy pathway in diabetic prostate and whether it is involved in C-peptide action. Forty adult male Wistar albino rats were separated into control group, diabetic group, diabetic + C-peptide and diabetic + C-peptide + 3-Methyladenine (autophagy inhibitor). Serum metabolic parameters and prostatic specific antigen (PSA) were measured. Markers of oxidative stress, inflammation, fibrosis, cell proliferation and cell autophagy were evaluated in prostate tissues using biochemical, western blotting and immunohistochemical techniques. C-peptide administration ameliorated the effects of diabetes on the prostate through its hypoglycaemic, antioxidant, anti-inflammatory, and antiproliferative effects which were reversed with autophagy inhibition. Thus, we concluded that C-peptide prevented the effects of diabetes on the prostate through stimulation of the autophagy pathway.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"419-431"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Saracatinib, a Src kinase inhibitor, enhances the renoprotective effect of metformin and losartan in diabetic nephropathy. Saracatinib是一种Src激酶抑制剂，可增强二甲双胍和氯沙坦对糖尿病肾病的肾保护作用。

IF 2.5 4区医学

Archives of Physiology and Biochemistry Pub Date : 2025-06-01 Epub Date: 2025-01-07 DOI: 10.1080/13813455.2024.2449404

Suzan A Khodir, Eman M Sweed, Mona A Kora, Nader G Zaki, Ghada S Amer, Omnia Ameen

{"title":"Saracatinib, a Src kinase inhibitor, enhances the renoprotective effect of metformin and losartan in diabetic nephropathy.","authors":"Suzan A Khodir, Eman M Sweed, Mona A Kora, Nader G Zaki, Ghada S Amer, Omnia Ameen","doi":"10.1080/13813455.2024.2449404","DOIUrl":"10.1080/13813455.2024.2449404","url":null,"abstract":"Objective: This research assesses renoprotective effects of saracatinib (Src) in diabetic nephropathy (DN) and the potential underlying processes.Materials and methods: Rats were divided into: control, DN, DN + Met + Los, DN + Met + Src, and DN + Met + Los + Src. Rats' ABP, urinary albumin, urinary nephrin, and creatinine clearance were assessed. Blood samples were collected for measuring glycaemic state parameters, renal functions, oxidative stress markers, inflammatory mediators, aldosterone, and lipid profile. Kidneys were extracted for KIM-1 and nephrin gene expression, H&E, Masson's trichrome staining, and immunohistochemical assessment.Results: Significant increases in ABP, urinary albumin and nephrin, glycaemic measurements, urea, creatinine, aldosterone, inflammatory cytokines, MDA, lipids, renal fibrosis, H scores of VEGF and TGF-β, and renal KIM-1 expression were related to DN. However, there was a significant decrease in creatinine clearance, GSH, and nephrin expression in DN group compared with control group.Discussion and conclusion: The combination of metformin (Met), losartan (Los), and Src repaired DN alterations. Adding Src to Met and Los is superior to using them alone.","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"467-482"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0