Metformin attenuates inflammation and improves insulin sensitivity in coculture of LPS-induced 3T3-L1 adipocytes and RAW 264.7 macrophages mediated by IRS-1/GLUT-4 pathway.

IF 2.5 4区医学 Q3 ENDOCRINOLOGY & METABOLISM

Archives of Physiology and Biochemistry Pub Date : 2025-02-03 DOI:10.1080/13813455.2025.2460102

Siska Andrina Kusumastuti, Dwi Aris Agung Nugrahaningsih, Mae Sri Hartati Wahyuningsih

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引用次数: 0

Abstract

Objective: Metformin is an anti-diabetic drug used to control blood glucose levels. The effects of metformin on insulin sensitivity in inflammation-induced adipocytes are not fully understood.This study aimed to explore the mechanism of metformin on insulin sensitivity enhancement in the coculture of LPS-induced 3T3-L1 adipocytes and RAW 264.7 macrophages.

Material and methods: Insulin resistance was induced in coculture cells using Lipopolysaccharide, followed by adding 25, 50, and 100 µg/ml of metformin for 24 h of incubation. Glucose consumption, GLUT-4, IRS-1, and IL-6 mRNA expressions were quantified.

Results: Metformin, starting at a concentration of 25 µg/ml, enhanced glucose consumption, upregulated GLUT-4 mRNA expression, and stimulated the expression of IRS-1 mRNA in coculture cells at 100 µg/ml of concentration. Additionally, Metformin inhibited inflammation by reducing IL-6 mRNA expression in coculture cells up to 100 µg/ml.

Discussion and conclusion: These findings suggest that metformin attenuated inflammation and improved insulin sensitivity in inflammation-induced adipocytes that may be mediated by the IRS-1/GLUT-4 pathway.

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二甲双胍可减轻lps诱导的3T3-L1脂肪细胞和IRS-1/GLUT-4途径介导的RAW 264.7巨噬细胞的炎症反应，提高胰岛素敏感性。

目的：二甲双胍是一种用于控制血糖水平的降糖药物。二甲双胍对炎症诱导的脂肪细胞胰岛素敏感性的影响尚不完全清楚。本研究旨在探讨二甲双胍对lps诱导的3T3-L1脂肪细胞与RAW 264.7巨噬细胞共培养中胰岛素敏感性增强的作用机制。材料与方法：用脂多糖诱导共培养细胞胰岛素抵抗，分别加入25、50、100µg/ml二甲双胍孵育24 h。定量葡萄糖消耗、GLUT-4、IRS-1和IL-6 mRNA表达。结果：二甲双胍浓度为25µg/ml时，增加葡萄糖消耗，上调GLUT-4 mRNA表达，并在浓度为100µg/ml时刺激共培养细胞中IRS-1 mRNA表达。此外，二甲双胍通过降低共培养细胞中IL-6 mRNA的表达，达到100µg/ml，从而抑制炎症。讨论和结论：这些发现提示二甲双胍减轻炎症并改善炎症诱导的脂肪细胞的胰岛素敏感性，这可能是由IRS-1/GLUT-4通路介导的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Physiology and Biochemistry ENDOCRINOLOGY & METABOLISM-PHYSIOLOGY

CiteScore

6.90

自引率

3.30%

发文量

期刊介绍： Archives of Physiology and Biochemistry: The Journal of Metabolic Diseases is an international peer-reviewed journal which has been relaunched to meet the increasing demand for integrated publication on molecular, biochemical and cellular aspects of metabolic diseases, as well as clinical and therapeutic strategies for their treatment. It publishes full-length original articles, rapid papers, reviews and mini-reviews on selected topics. It is the overall goal of the journal to disseminate novel approaches to an improved understanding of major metabolic disorders. The scope encompasses all topics related to the molecular and cellular pathophysiology of metabolic diseases like obesity, type 2 diabetes and the metabolic syndrome, and their associated complications. Clinical studies are considered as an integral part of the Journal and should be related to one of the following topics: -Dysregulation of hormone receptors and signal transduction -Contribution of gene variants and gene regulatory processes -Impairment of intermediary metabolism at the cellular level -Secretion and metabolism of peptides and other factors that mediate cellular crosstalk -Therapeutic strategies for managing metabolic diseases Special issues dedicated to topics in the field will be published regularly.