实验性代谢综合征大鼠肝脏和胆胰轴中胰岛素和胰高血糖素生成细胞的研究。

IF 2.5 4区医学 Q3 ENDOCRINOLOGY & METABOLISM

Archives of Physiology and Biochemistry Pub Date : 2025-06-28 DOI:10.1080/13813455.2025.2503482

M Gulubova, A Tolekova, D Berbatov, I Stefanov, D Chonov, N Aydoglu

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引用次数: 0

摘要

背景：胰岛素生成细胞（IPCs）的产生作为糖尿病治疗的细胞替代疗法具有挑战性。目的：我们评估了肝细胞、胆道周围腺（PBGs）和肝窦内皮细胞（LSECs）中胰岛素阳性（胰岛素+）和胰高血糖素阳性（胰高血糖素+）细胞的存在。材料与方法：Wistar大鼠饲喂含15%果糖溶液的日粮3个月。组织样本用胰岛素、胰高血糖素、生长素、生长抑素、PDX1和SOX9抗体进行免疫组织化学处理。研究血糖水平和血脂。结果：大鼠中央静脉周围可见胰高血糖素+和Ins+肝细胞。在PBGs中检测到Ins+和胰高血糖素+内分泌细胞（ECs）。LSECs显示胰岛素+和胰高血糖素+细胞膜。治疗大鼠LSECs细胞核sox9阳性。结论：果糖诱导代谢综合征实验显示肝外胆道和肝细胞中出现Ins+和胰高血糖素+ ECs。有趣的是，LSECs的SOX9+核被观察到。

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Insulin- and glucagon-producing cells in the liver and biliary-pancreatic axis of rats with experimentally induced metabolic syndrome.

Context: The generation of insulin-producing cells (IPCs) as cell replacement therapy for diabetes treatment is challenging.

Objective: We have evaluated the presence of insulin-positive (insulin⁺) and glucagon-positive (glucagon⁺) cells in hepatocytes, peribiliary glands (PBGs), and liver sinusoidal endothelial cells (LSECs).

Materials and methods: Wistar rats are subjected to a diet including administration of 15% fructose solution for 3 months. Tissue samples are processed for immunohistochemistry with antibodies against insulin, glucagon, ghrelin, somatostatin, PDX1, and SOX9. Blood glucose levels and lipid profile are investigated.

Results: In treated rats, Ins⁺ and glucagon⁺ hepatocytes are found around central veins. In PBGs, Ins⁺ and glucagon⁺ endocrine cells (ECs) are detected. LSECs show insulin⁺ and glucagon⁺ cellular membranes. The nuclei of LSECs in treated rats are SOX9-positive.

Conclusions: Our experiment of fructose-induced metabolic syndrome shows the appearance of Ins⁺ and glucagon⁺ ECs in extrahepatic biliary pathways and hepatocytes. Interestingly, SOX9⁺ nuclei of LSECs are observed.

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来源期刊

Archives of Physiology and Biochemistry ENDOCRINOLOGY & METABOLISM-PHYSIOLOGY

CiteScore

6.90

自引率

3.30%

发文量

期刊介绍： Archives of Physiology and Biochemistry: The Journal of Metabolic Diseases is an international peer-reviewed journal which has been relaunched to meet the increasing demand for integrated publication on molecular, biochemical and cellular aspects of metabolic diseases, as well as clinical and therapeutic strategies for their treatment. It publishes full-length original articles, rapid papers, reviews and mini-reviews on selected topics. It is the overall goal of the journal to disseminate novel approaches to an improved understanding of major metabolic disorders. The scope encompasses all topics related to the molecular and cellular pathophysiology of metabolic diseases like obesity, type 2 diabetes and the metabolic syndrome, and their associated complications. Clinical studies are considered as an integral part of the Journal and should be related to one of the following topics: -Dysregulation of hormone receptors and signal transduction -Contribution of gene variants and gene regulatory processes -Impairment of intermediary metabolism at the cellular level -Secretion and metabolism of peptides and other factors that mediate cellular crosstalk -Therapeutic strategies for managing metabolic diseases Special issues dedicated to topics in the field will be published regularly.