Archives of Razi Institute最新文献

{"title":"Short-term Effects of Surgical Sterilization on Urinary PH and Specific Gravity, Blood Urea Nitrogen, Serum Creatinine, and Urinary Protein to Creatinine Ratio in Sexually Intact Male and Female Dogs.","authors":"M Yousefi Ghadikolaei, M Ahmadi-Hamedani, H R Moslemi","doi":"10.32592/ARI.2024.79.4.873","DOIUrl":"https://doi.org/10.32592/ARI.2024.79.4.873","url":null,"abstract":"<p><p>Surgical sterilization is a widely accepted method to reduce the birth of unwanted dogs and reducing the incidence of reproductive diseases. However, the sterilization of domestic dogs has generated debate among veterinarians and owners about its effects on urinary parameters. The purpose of this study was to evaluate the short-term effects of surgical sterilization on urinary parameters in healthy adult sexually intact male and female dogs. Ten healthy adult sexually intact mongrel dogs (5 males and 5 females) weighing 15 to 20 kg participated in this study. Only those dogs which were at least 1yearold included in this study to ensure that they had reached sexual maturity. If a dog's urine sample is abnormally colored or milky, has a pH greater than 7.5, and a positive bacterial culture is confirmed, the dog will be excluded. Physical, CBC, and biochemical examinations, such as measurement of blood urea nitrogen (BUN) and serum creatinine (Cr) concentrations and urinalysis (including determination of urine pH and specific gravity (USG) measurement, calculation of the urine protein/ creatinine ratio (UPC), and dipstick evaluation) were performed on each dog. At least 15 days after the sterilization surgery, the dogs underwent physical examination, CBC, biochemistry, and urinalysis at least 15 days later. Urine samples were collected by cystocentesis and stored at 4°C for analysis. After centrifugation at 3000 g for 5 minutes, the urinalysis was performed within 4 hours of collection. There were no significant differences (P>0.05) between the mean BUN and Cr, UPC, USG, and urine pH before and after ovariohysterectomy and castration. The study found that castration and ovariohysterectomy did not affect short-term urinary parameters in healthy adult sexually intact male and female dogs. However, it is uncertain how sterilization affects urinalysis results in sexually intact dogs, and more research with larger sample sizes is needed to determine the impact.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"79 4","pages":"873-880"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the effect of chamomile flower powder (CFP) on performance traits, lipid profile and morphology jejunum of Japanese quail from 7 to 35 days of age.","authors":"F Ahmadi, K Karimipanah, M Balali","doi":"10.32592/ARI.2024.79.4.769","DOIUrl":"https://doi.org/10.32592/ARI.2024.79.4.769","url":null,"abstract":"<p><p>This study was conducted to investigate the effect of Chamomile flower powder (CFP) on performance traits, lipid profile and morphology jejunum of Japanese quail from 7 to 35 days of age. A total of 200 7-day-old male Japanese quail were as randomly distributed to 5 treatments, with 4 replicates, and 10 birds in each pen. The experimental treatments included (T1) a control diet (treated with basal diet only), (T2-T5) basal diet supplemented with of 0.5, 1, 1.5 and 2 % of CFP, respectively. Live body weight (LBW) and Feed intake (FI) were determined weekly, and then feed conversion ratio (FCR) was calculated. At the end of the study 5 birds with an average weight close to the same treatment were selected from each experimental treatment andblood and intestine (jejunum section) samples were collected at 35 days of age for further analysis. Blood samples were collected from the axillary vein and poured into test tubes without heparin. Blood serum was centrifuged and stored at -20ºC until testing. Then, the birds were slaughtered and after opening the abdominal cavity, a section (3 cm) from the middle part of the jejunum was separated and kept in 10% formalin (pH=7.2) to study the jejunum morphology. The results showed that live body weight, feed intake and feed conversion ratio were significantly improved (P<0.05) in the birds CFP-fed compared to the control. The addition of 1% and 1.5% CFP (T3 and T4) to the basal diet showed the best performance results. The level of HDL was significantly (P<0.05) decreased in the birds fed CFP (T3 and T4) compared to the control. Other lipid profile parameters were not significantly (P>0.05) affected by the CFP used, although the results showed a numerical trend of decrease compared to the control. Villus height and depth crypt of the bird jejunum significantly increased (P<0.05) compared to the control. In conclusion, these results suggest that dietary supplementation with 1% and/or 1.5% CFP has beneficial effect on growth performance, lipid profile and jejunum morphometric of quails.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"79 4","pages":"769-776"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatic development of a recombinant trivalent synthetic protein construct using PTXa, Tox, and TetX toxins as a DTP vaccine candidate.","authors":"Z Salahi, M Noofeli, M M Ranjbar, M Bagheri, M Esmaelizad, M Niakan","doi":"10.32592/ARI.2024.79.4.777","DOIUrl":"https://doi.org/10.32592/ARI.2024.79.4.777","url":null,"abstract":"<p><p>Traditionally, diphtheria-tetanus-pertussis (DTwP or DTaP) as pediatric vaccines are produced from the corresponding inactivated toxins or whole -cell pathogenic bacteria of Corynebacterium <i>diphtheria</i> toxin (Tox), <i>Clostridium tetani</i> toxin (TetX) and <i>Bordetella pertussis</i>. There are major concerns in the classic or acellular DTP (DTaP) vaccine production processes from native live bacterial sources as it may raise concerns on adverse effects and safety issues, complexity of the purifications for each agent as well as cost. Here, we designated a recombinant multi-epitope vaccine candidates by vaccino-informatics study to address the mentioned issues and to develop a single trivalent fusion protein as a potent recombinant DTP vaccine. To achieve these goals, stages of immune-bioinformatics were retrieved using proteinaceous toxins sequences, predicting secondary/tertiary structure and transmembrane topology, energy minimization, and model validation. Then, conformational and linear Bcell epitope prediction by several servers, mapping of consensus linear/discontinuous immunogenic regions and construction synthetic fusion vaccine candidates in respect to optimal immunogenic, physicochemical properties and high expression in prokaryote host were achieved. Finally, reverse translation, codon optimization, addition of cloning tags for pet 28a vector and optimization of physicochemical properties of synthetic trivalent fusion protein were performed. Through various hybrid immuno-informatics and structural bioinformatics analysis of predicted and experimental epitopes finally, 12 new consensus highly immunogenic linear and discontinuous epitopes in Tox, TetX and PTXa proteins were selected. The peptide sequences of these immunogenic regions were as follows: PTXA (AA34-64, AA184-256 and AA98-116), Tox (AA47-76, AA117-159, AA515-557 and AA245-265) and TetX (AA226-249, AA819-844, AA923-967, AA1009-1067 and AA1225-1315). In addition, the characteristics of the recombinant trivalent fusion construct were; 546 residue length, soluble (Grand average of hydropathicity (GRAVY) was -0.475), estimated half-life was >10 hours in <i>Escherichia coli</i>, pI 5.94 (a little acidic), stable protein (The instability index (II) 35.58) as well as thermally stable (Aliphatic index (AI) 71.67). The putative antigenic epitopes from different organisms in a single protein, as in the current study, will possibly improve the protective efficacy as novel potent, safe, cheap and broad-spectrum vaccines for better prevention of diphtheria, tetanus and pertussis infections in the future.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"79 4","pages":"777-788"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004056/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Malondialdehyde Levels Decreased Along with the Alleviating Effects of Marrubium Parviflorum on Morphine Withdrawal Syndrome in Rats.","authors":"K Fekri, F Hooshangi, A Parvizpur, M Charkhpour, S Hamedeyazdan","doi":"10.32592/ARI.2024.79.4.833","DOIUrl":"https://doi.org/10.32592/ARI.2024.79.4.833","url":null,"abstract":"<p><p>As a major concern for the clinicians, better treatment of the patients hospitalized to stop opioid abuse has always been a target for the researchers working in this field. On the other hand, the therapeutic potential of medicinal plants has become of great interest to both researchers and consumers in recent years. Among the plants, we can mention those belonging to the genus Marrubium, which have been reported to exert many therapeutic effects. The aim of this research was to investigate the effect of Marrubium parviflorum on morphine withdrawal syndrome and its possible relationship with malondialdehyde (MDA), the indicator of lipid peroxidation that is elevated during the syndrome. To perform this study, 48 rats were divided into 6 groups as follows: 1) Saline-Saline 2) Saline-Morphine; 3, 4, 5) Different doses of the Extract-Morphine (10, 20 and 40 mg.kg^-1); 6) and the most effective dose of the extract-Saline. To evaluate the withdrawal syndrome, the increasing doses of morphine were injected subcutaneously for 9 days followed by a single dose of naloxone (4 mg.kg^-1, i.p.). Withdrawal symptoms were then assessedand the total withdrawal score (TWS) was calculated. On the other hand, to confirm the efficacy biochemically and to investigate the possible relationship between the observed effects and lipid peroxidation, blood samples were taken for malondialdehyde (MDA) measurement. According to the data, administration of the extract (in two higher doses) significantly alleviated the syndrome-related behavioral signs as well as MDA levels significantly. Overally, based on the results, aerial parts of Marrubium parviflorum seem to be beneficial for better coping with morphine withdrawal syndrome through complex pathways such as suppressing lipid peroxidation, further preclinical and clinical studies are required.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"79 4","pages":"833-842"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004045/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A phylogenetic landscape of <i>Ornithobacterium rhinotracheale</i> isolates from poultry in Iran presented based on 16S rRNA gene analysis.","authors":"S Shahsavandi, N Ghodsian, M M Ebrahimi, R Ghadimipour, V Karimi","doi":"10.32592/ARI.2024.79.4.857","DOIUrl":"https://doi.org/10.32592/ARI.2024.79.4.857","url":null,"abstract":"<p><p><i>Ornithobacterium rhinotracheale</i> (ORT) is one of the most important bacterial agents of respiratory diseases causing significant economic losses to the poultry industry. The partial 16S rRNA gene sequences of ORT isolates originating from different geographical areas in Iran have been deposited in the GenBank database from 2011 to the present. The objectivepresent study was to evaluate the molecular evolution of ORT isolates. to this end, a dataset comprising 16S rRNA gene sequences of 48 field strains derived from outbreaks on poultry farms and one reference strain was subjected to phylogenetic and evolutionary analysis using a series of substitution pattern homogeneity, nucleotide diversity, and neutrality assays. The phylogenetic tree, based on the 16S rRNA gene, revealed the presence of a large clade, designated as A, and a distinct clade B, which included six sequences derived from the recent ORT isolates. The majority of the isolates exhibited homogeneityand clustered together,irrespective of their geographical origin. The evolutionary inference indicated that a molecular divergence influences the genetic diversity of ORT isolates. The Tajima's D statistic yielded a negative value of -1.46, while the R-value for transition/transversion bias was 1.399. Additionally, the dN/dS ratio was estimated to be higher for clade B. These findings suggest that recent ORT isolates in Iran are subjected to increasing selection pressure on the 16S rRNA gene of recent ORT isolates in Iran. The phylogeny analysis and evolutionary affiliations of the ORT 16S rRNA gene are expected to prove effective in the monitoring of the strains for antibiotic resistance and selection pressure. These phylogenetic relationships have the potential to be determinative in the selection of the best vaccine candidate strain.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"79 4","pages":"857-863"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Absorbents therapy, as a conservative option, can improve kidney function in chronic kidney disease.","authors":"B Abedi, R Karimian, Z Bahari, M Iman","doi":"10.32592/ARI.2024.79.4.695","DOIUrl":"https://doi.org/10.32592/ARI.2024.79.4.695","url":null,"abstract":"<p><p>Chronic kidney disease (CKD), also called chronic kidney failure, is increasingly recognized as a global public health problem in the entire world. It is characterized by slow, progressive, and irreversible loss in kidney physiology. Today, the prevalence of CKD is increasing dramatically. CKD can affect almost every organ system, including the cardiovascular system. Many treatments have been attempted for CKD, such as renal transplantation, hemodialysis (HD), and peritoneal dialysis (PD). At the end stage of CKD, HD is the most widely used therapy throughout the world. However, these options can decrease volume expansion and uremic solute retention and also increase patient survival. Furthermore, there are certain complications associated with the use of these methods. Previous studies have reported that the main side effects are headaches, muscle cramps, abdominal pain, hypotension, hypertension, vomiting, and constipation. Therefore, the investigation for better and more convenient dialysis techniques should continue, as well as the search for a better material to enhance the clearance of nitrogenous waste products from the body. The intestine has a significant effect on the clearance of nitrogenous waste products from the body, making it a potentially appropriate site for CKD management. The potential mechanism of the intestinal dialysis technique is that it can absorb excess fluids, uremic toxins, and electrolytes within the gastrointestinal (GI) tract and exert them in the feces before they can be absorbed into the blood. In the present review, we will focus on different absorbents as a conservative treatment to remove uremic waste metabolites from the GI tract for the improvement of kidney function in CKD.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"79 4","pages":"695-700"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of HIV, HBV, and HCV infections and high-risk behaviors among women referred to drop-in centers in Lorestan Province, western Iran.","authors":"H Mokhayeri, B Hasanvand, M Birjandi, H Mirzaei, P Sasaei, S Zamani, S Bajlan, A Akbari, S Khanizadeh","doi":"10.32592/ARI.2024.79.3.623","DOIUrl":"10.32592/ARI.2024.79.3.623","url":null,"abstract":"<p><p>Human immunodeficiency virus (HIV), hepatitis B virus (HBV), and hepatitis C virus (HCV) are known as the most common blood-borne viral infections worldwide. Individuals referring to drop-in centers (DICs) are considered high-risk people exposed to infection with blood-borne viruses. The purpose of this study was to investigate the prevalence of HIV, HBV, and HCV infections among women referred to DICs in Lorestan Province, western Iran. During this cross-sectional study, after obtaining informed consent and completing a demographic form, two blood samples were collected from 118 women referred to Lorestan DICs to be evaluated for HIV, HBV, and HCV infections. Accordingly, the samples were first screened by using the most common serological methods and then by Real-Time polymerase chain reaction (PCR) to detect viral genomes. The results were analyzed using Chi-square statistical test and binary logistic regressions. Out of the 118 blood samples, 8 (6.8%), 4 (3.4%), and 10 (8.5%) subjects tested positive for HIV, HBV, and HCV infection, respectively. Furthermore, our study showed that 5 (4.2%) subjects were positive for HCV/HIV coinfection and 3 (2.5%) cases for HBV/HCV coinfection. Moreover, our data revealed that incarceration and STDs were the most important risk factors for HIV (odds ratio [OR]=15.27; 95% confidence interval [CI]: 3.74-62.89; <i>P</i>=0.01) and HBV (OR=16; 95% CI 1.57-42.34); <i>P</i>=0.018) infections, respectively. Our results suggested that women referring to DICs are at risk of infection with blood-borne viruses due to risky behaviors. Thus, precise diagnostic testing and implementation of preventive methods against blood and sexually transmitted infections are urgent in these high-risk groups.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"79 3","pages":"623-628"},"PeriodicalIF":0.0,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11682500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Characterization of <i>Strongyloides Stercoralis</i> in Mazandaran Province, North of Iran.","authors":"A Dalimi, F Jaffarian","doi":"10.32592/ARI.2024.79.3.513","DOIUrl":"10.32592/ARI.2024.79.3.513","url":null,"abstract":"<p><p><i>Strongyloides stercoralis</i> is a parasitic nematode that lives in the mucosa of the small intestine and causes strongyloidiasis in humans. Mazandaran is among the endemic areas of this parasite in Iran. For detecting <i>S. stercoralis</i> larvae in stool samples, various techniques, such as PCR technique have been used. The present study was conducted to determine the molecular characteristics of <i>S. stercoralis</i> collected from residents of Mazandaran, Northern Iran. From April to September 2017, a number of 2,195 samples of human feces were collected from different regions of Mazandaran province. First, all stool samples were tested using the formalin-ether method. Then, <i>S. stercoralis</i>-positive stool samples and 300 random samples were selected for molecular study. A set of primer pairs for conventional PCR was used in a PCR reaction to amplify the mitochondrial cytochrome c oxidase subunit 1 (<i>Cox1</i>) gene. To confirm the results of PCR, positive samples were sent for sequencing. The sequence was compared with reference sequences from GenBank. Phylogenetic relationships of the <i>Cox1</i> gene of <i>S. stercoralis</i> inferred by the maximum likelihood algorithm. According to our results, in the stool test with the formal ether method, 21 (0.95%) stool samples were found to be positive for <i>S. stercoralis</i>, and 162 (38.7%) samples were positive for other parasites. All 21 positive samples were confirmed as <i>S. stercoralis</i> by PCR method. The sequence of the samples overlapped 99% with <i>S. stercoralis</i> in the Genbank. Our results showed that conventional PCR could detect all the microscopically positive samples.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"79 3","pages":"513-518"},"PeriodicalIF":0.0,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11682514/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of ultrasound on the physical, biochemical, antioxidant, and antimicrobial properties of industrial Iranian honey.","authors":"S Rabbani, Saa Anvar, S Allahyaribeik, B Jannat, H Ahari","doi":"10.32592/ARI.2024.79.3.475","DOIUrl":"10.32592/ARI.2024.79.3.475","url":null,"abstract":"<p><p>The aim of this study was to investigate the effects of ultrasound at two different frequencies, namely 30 kHz and 42 kHz, on various aspects of industrial Iranian honey, including its physical, biochemical, antioxidant and antimicrobial properties. Samples were subjected to ultrasound treatment at 30 kHz or 42 kHz for a duration of 1, 5 or 10 minutes at temperatures of 20 °C or 45 °C, respectively. The following parameters were then evaluated on days 1, 30, 90, and 180: HMF content, pH, acidity, proline concentration, total number of aerobic mesophilic bacteria, diastase activity, moisture content, sucrose concentration, fructose concentration, glucose concentration, fructose- glucose ratio, ABTS (antioxidant activity) content, number of osmophiles, phenol concentration, reducing sugar concentration and total sugar concentration. It is noteworthy that both treatment groups exposed to ultrasound showed changes in these parameters. Specifically, the group treated with ultrasound at a frequency of 42 kHz showed a decrease in moisture content, pH, acidity, fructosetoglucose ratio, total sugar concentration, clostridium count, total microbial count, mold count, osmophiles countand HMF content; as well as an increase in diastase activity, proline and phenol concentration. These changes were more pronounced on days 90 and 180. Ultrasound treatment at a frequency of 42 kHz for a duration of 10 minutes at a temperature of 45 °C has the potential to improve the quality and shelf life of industrial honey. Consequently, this technique can be used in the food industry to obtain a better product that is more suitable for export.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"79 3","pages":"475-490"},"PeriodicalIF":0.0,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11682496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Serum Levels of Vitamin D and Selected Biochemical and Hematological Parameters in Rats Post-Immunized with Inactivated COVID-19 Virus.","authors":"M Mehravaran, M Ahmadi Hamedani, K Kafshdouzan, H Staji, M H Yousefi","doi":"10.32592/ARI.2024.79.3.609","DOIUrl":"10.32592/ARI.2024.79.3.609","url":null,"abstract":"<p><p>Serum levels of vitamin D (VD) are inversely correlated with the incidence or severity of COVID-19. This study aimed to investigate the effects of inactivated COVID-19 virus (ICoV-19) immunization on VD levels, as well as biochemical and hematological parameters in adult male Sprague-Dawley rats. Twenty rats were randomly divided into two groups of 10: the control group (group I) and the ICoV-19-receiving group (group II). Aluminum hydroxide and a single dose of ICoV-19 (10⁷ TCID50 of the HB02 strain of the COVID-19 virus) were administered subcutaneously to rats in groups I and II, respectively. After two weeks (on day 14), booster doses were administered to both groups. Blood samples were collected from the rats in both groups primarily for the experiment and 21 days after the second administration (on day 35). Samples were then stored frozen until analysis. There were no significant differences in VD levels between the two groups on day 0, but on day 35, VD levels in group II had significantly decreased, compared to group I (<i>P</i><0.05). Additionally, group II had higher concentrations of fibrinogen, blood urea nitrogen, creatinine, and lactate dehydrogenase activity than group I (<i>P</i><0.05). Group II also showed a significant increase in neutrophil counts and neutrophil-to-lymphocyte ratio after 35 days (<i>P</i><0.05), while lymphocyte counts decreased. These findings suggest that VD may play a role in preventing COVID-19 and can thus be a potential candidate for managing and controlling the disease.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"79 3","pages":"609-616"},"PeriodicalIF":0.0,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11682505/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}