Archives of Razi Institute最新文献

{"title":"Investigation of Antibiotic Susceptibility Patterns in Bacteria Isolated During a Male Sheep Castration Surgery.","authors":"Akbari Zahra, Zalikani Neli, Babaei Mohaddeseh","doi":"10.32592/ARI.2025.80.4.1075","DOIUrl":"https://doi.org/10.32592/ARI.2025.80.4.1075","url":null,"abstract":"<p><p>There are different antibiotic resistance profiles among <i>Yersinia</i> spp. This pathogenic bacterial species causes yersiniosis worldwide, requiring testing of the organism's susceptibility in the local environment. An antibiotic susceptibility profile of a <i>Yersinia</i> spp. isolated from a castration surgical site was analyzed to provide insight into selecting appropriate antibiotics to treat <i>Yersinia</i> spp. infections while addressing antibiotic resistance issues effectively. The surgical site was swabbed before castration, and cultures were performed. Samples from the surgical site were taken after the procedure, cultured, and then incubated. CLSI 2020 guidelines were followed for interpreting antibiotic susceptibility tests. The Kirby-Bauer disk diffusion method was applied to better understand antibiotic susceptibility and resistance patterns, and a zone of inhibition measurement was used to determine the antibiotic effectiveness. Staining and microscopic examination of swab samples after surgery revealed a single colony of Gram-negative bacteria. Laboratory tests confirmed that the isolated Gram-negative bacilli were indeed <i>Yersinia</i> spp. Methyl Red and Voges-Proskauer tests showed negative results, while Citrate utilization testing demonstrated a positive result. A positive result was obtained for <i>Yersinia</i> spp. in the glucose fermentation test. Specifically, nitrofurantoin showed a significant zone of inhibition of over 17 mm, and gentamicin showed a zone of inhibition greater than 27 mm. However, resistance to ampicillin (11 mm), ceftriaxone, and cefazolin was observed. Due to the observed resistance to antibiotics, our results indicate that nitrofurantoin and gentamicin are likely to be the best options for treating Yersinia spp., in contrast to ampicillin, cefazolin, and ceftriaxone, which may be unsuitable due to resistance.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"80 4","pages":"1075-1080"},"PeriodicalIF":0.0,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the efficacy of in-house-produced AgB with a domestic commercial kit for the serodiagnosis of human cystic echinococcosis by the ELISA method.","authors":"Mohammadzadeh Tahereh, Arsalani Saideh, Hafezi Fatemeh, Sadjjadi Seyed Mahmoud, Heidari Soudabeh, Sharifzadeh Parviz, Darabi Enayat","doi":"10.32592/ARI.2025.80.4.1023","DOIUrl":"https://doi.org/10.32592/ARI.2025.80.4.1023","url":null,"abstract":"<p><p>Cystic echinococcosis (CE) is a common zoonotic infection caused by the metacestode form of <i>Echinococcus granulosus</i>. The disease is found worldwide, and Iran is considered an endemic area. Depending on the affected organ, the disease presents with different clinical signs and symptoms. Humans are regarded as dead-end intermediate hosts. CE cysts commonly develop in the liver and lungs, while they are less frequently found in other organs. Timely diagnosis of this disease is essential in humans. Diagnosis relies on imaging methods (X-ray, ultrasound, MRI, and CT scan), complemented by serological testing. The present study used the indirect ELISA method to compare the efficacy of in-house-produced antigen B (AgB) and a domestic commercial kit. Hydatid cyst fluid (HCF) was extracted from the liver of sheep infected with a cystic echinococcosis cyst, followed by the preparation of AgB. It was used in the indirect ELISA test. A total of 142 sera, consisting of 36 samples from patients with pathologically confirmed CE, 46 samples from patients with other parasitic diseases, and 60 samples from healthy individuals, were examined using in-house-produced AgB and a domestic commercial ELISA kit. Our findings revealed the sensitivity and specificity of the indirect ELISA using in-house AgB to be 86.11% and 95.28%, respectively. The commercial kit demonstrated a sensitivity of 77.78% and a specificity of 99.06%. Although both the in-house-produced AgB and the commercial kit showed considerable and relatively similar diagnostic efficiency, the study results showed a higher sensitivity and validity of AgB compared with the commercial ELISA kit.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"80 4","pages":"1023-1028"},"PeriodicalIF":0.0,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Detection of Herpesvirus, Adenovirus, and Circovirus and Their Associated Histopathological Lesions in the Pigeons of Mashhad, Iran.","authors":"BehrouziNasab Omid, Razmyar Jamshid, Kalidari GholamAli, Nourani Hossein, KafiMashhadi Reza","doi":"10.32592/ARI.2025.80.4.979","DOIUrl":"https://doi.org/10.32592/ARI.2025.80.4.979","url":null,"abstract":"<p><p>Pigeon racing is a popular sport, where trained pigeons compete against each other in flying competitions. Furthermore, pigeons serve as laboratory specimens for scientific research and experiments. Additionally, many people keep pigeons as companion animals, enjoying their company and unique characteristics. The pigeon holds a significant cultural and religious significance, particularly in Islamic countries. Pigeons are susceptible to a range of viral pathogens that can impact their health and performance. This study focuses on three crucial viruses affecting pigeons: Pigeon Adenovirus, Pigeon Circovirus, and Pigeon Herpes virus. To detect these viruses in pigeons, the researchers utilized a method called Polymerase Chain Reaction (PCR). They collected liver samples from deceased pigeons referred to the Veterinary Hospital of Ferdowsi University in Mashhad, Iran. The researchers detected DNA from the samples and prepared histopathological slides following specific protocols. This study confirmed the presence of adenovirus in 15.5%, circovirus in 100%, and herpes virus in 22.5% of the studied pigeons. AdHistopathological examination was conducted on 43% of the samples. Although only one sample (3.3%) exhibited typical inclusion bodies, nearly all the samples showed varying degrees of pathological changes, including congestion, hemorrhage, and necrosis. The present study is among the few investigations into 3 important viruses in pigeons and it is necessary to pay special attention to its results and carry out additional works. Detecting 100% of the livers of sick pigeons as infected with circovirus can also be a significant result, because other manuscripts have not reported such severe contamination.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"80 4","pages":"979-986"},"PeriodicalIF":0.0,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anatomical and histological investigation of the effect of superoxide dismutase on eye muscles in Tuj sheep.","authors":"Doğan Gülseren Kirbaş, Yildiz Sevda Eliş, Sari Ebru Karadağ, Ölmez Mükremin, Şahin Tarkan","doi":"10.32592/ARI.2025.80.4.879","DOIUrl":"https://doi.org/10.32592/ARI.2025.80.4.879","url":null,"abstract":"<p><p>In this study, 16 eye muscles belonging to 8 male Tuj breed sheep, aged around 2-3 months, were used. The research was carried out on two groups: control and study. The mean and standard deviation values of all measurements obtained as a result of anatomical examinations, and the differences between the control and study groups, were determined by the Independent Samples T-test in the SPSS package program. While no additive was added to the control group diet, an antioxidant feed additive rich in superoxide dismutase was added to the study group diet. As a result of the anatomical evaluation, the longest muscle in the control group was <i>musculus obliquus dorsalis</i> (34.43 mm), while the shortest muscle was <i>musculus retractor bulbi</i> (21.74 mm). In the study group, the longest muscle was <i>musculus rectus dorsalis</i> (34.5 mm), while the shortest muscle was <i>musculus retracor bulbi</i> (22.27 mm). Mallory's modified triple staining (Triple) was applied for histolohical examination. In this study, it was observed that the musculus rectus dorsalis, musculus rectus ventralis, musculus rectus medialis, musculus rectus lateralis, musculus obliquus dorsalis, musculus obliquus ventralis and musculus retractor bulbi muscles obtained from Tuj sheep in both the study and control groups consisted of a striated skeletal muscle system and showed transverse striations. What was clearly seen in both anatomical and histological evaluations was that superoxide dismutase had a positive effect on the <i>musculus rectus medialis</i>, one of the eye muscles. It is thought that the present study will contribute to intraocular eye surgery operations and research relating eye diseases to nutrition.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"80 4","pages":"879-886"},"PeriodicalIF":0.0,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13125765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virus-Like Particles (Vlps) from Synthesis to Targeted Drug Delivery, Vaccine Approaches, and Gene Therapy.","authors":"Esmaeili Gouvarchin Ghaleh Hadi, Mahabadi Mostafa, Farzanehpour Mahdieh, Zahiri Ali, Mirzaei Nodoushan Majid","doi":"10.32592/ARI.2025.80.4.833","DOIUrl":"https://doi.org/10.32592/ARI.2025.80.4.833","url":null,"abstract":"<p><p>Virus-like particles (VLPs) are spontaneously generated from viral capsid proteins. VLPs imitate genuine viruses visually and physiologically, but lack viral DNA. Various VLP designs provide structural and functional appeal. Spontaneous polymerization of viral capsid proteins may result in the formation of VLPs with geometrical symmetry, which are often icosahedral, spherical, or rod-like. Moreover, functionalized VLPs may precisely target cancer cells and recruit macrophages to destroy them. The ability to target tumors for therapeutic drug delivery through VLP-based delivery platforms in novel and intriguing aspects related to cancer treatment is the primary goal of VLP design. Cancer therapies require precise targeting of diagnostic or therapeutic elements to tumor cells while avoiding healthy cells and tissues. VLPs offer an innovative approach as site-specific drug delivery systems, reducing systemic toxicity and minimizing injury to healthy cells. Immunotherapy, which boosts the host's immune system, has fewer side effects. Cancer vaccines aim to induce an immune response that provides protection against tumor cells. Due to their naturally fitted particle size and repetitive structural order, VLPs may be employed as a vaccine without any adjuvant. Recombinant VLP structures can be enhanced by including antigenic epitopes of viruses or different disease-related antigens, and targeting peptides to the interior and exterior surfaces, making them potential tools for future immunizations with preventive and regenerative qualities. Additionally, VLP-based delivery strategies may enhance immunogenicity and provide a more effective and safer approach to managing solid cancers with fewer side effects compared to chemotherapy or radiation. However, the production of chimeric VLPs still faces challenges, such as the need for more reliable preclinical animal models and associated costs. Despite these obstacles, ongoing research will improve VLP-based technologies and increase their potential advantages. This review aims to provide basic information on VLPs and outline current studies on their use as drug and vaccine delivery systems in different cancers, highlighting their potential as a promising cancer treatment strategy. The key terms in the literature search-including drug delivery, gene therapy, multi-capsid VLPs, and virus-like particles (VLPs)-were searched in international databases, namely Web of Science, PubMed, and Scopus from 2003 to 2022.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"80 4","pages":"833-842"},"PeriodicalIF":0.0,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122418/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Lactobacillus</i> and <i>Bifidobacterium</i> of Patients with Strongyloidiasis Compared with the Control Group.","authors":"Kazeminejad Arezou, Kia Eshrat Beigom, Bagheri-Josheghani Sareh, Fahimfar Noshin, Darabi Enayat, Fakhrieh-Kashan Zohreh","doi":"10.32592/ARI.2025.80.4.917","DOIUrl":"https://doi.org/10.32592/ARI.2025.80.4.917","url":null,"abstract":"<p><p>In individuals with compromised immune systems, strongyloidiasis disease can lead to disseminated infections that can be fatal if diagnosis and treatment are delayed. The human gut is composed of numerous bacteria that play essential roles in the development of acquired immunity, and protection against pathogenic factors. This case-control study was conducted on individuals who were referred to the Diagnostic Laboratory of Strongyloidiasis in the School of Public Health, Tehran University of Medical Sciences. After DNA extraction from fecal samples, the 16SrRNA gene was examined using Real-time PCR. The levels of <i>Lactobacillus acidophilus</i> and <i>Bifidobacterium bifidum</i> were calculated in both groups (one group consisted of individuals suspected of strongyloidiasis, compared with the other group with no underlying disease). Finally, the collected data were analyzed. Out of 28 participants in this study, 16 (57%) were men and 12 (43%) were women, with ages ranging from 43 to 76 years. A statistically significant relationship was observed between underlying diseases, vegetable washing practices, and clinical symptoms of strongyloidiasis. DNA extraction from the fecal samples was performed using a DNA Extraction kit. The average level of <i>L. acidophilus</i> and <i>B. bifidum</i> were (4.07250±3.132533) 10¹²× and, (6.12857±3.519169) 10¹²× in the case group, respectively, which were lower compared to the control group. However, no significant association was found between the bacterial levels in the case and control groups and the incidence of strongyloidiasis (<i>p</i>>0.05), the control group had (7.04733± 6.542372) 10¹²× and (8.36643± 4.754185) 10¹²×, respectively. The odds ratio for <i>L. acidophilus</i> and <i>B. bifidum</i> were 1.13 and 1.14, respectively. It was observed that for each increase of 10¹² in the microliter of <i>L.acidophilus</i> and <i>B. bifidum</i> in the individual's intestines in areas endemic for strongyloidiasis, the chances of contracting this disease decreased by 13% and 14%, respectively. Future studies with a larger sample size, considering age, gender and other physiological factors related to strongyloidiasis, are suggested.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"80 4","pages":"917-925"},"PeriodicalIF":0.0,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological Study and Phylogenetic analysis of Common Pathogenic Viruses of Honeybee in Apiaries of Iran.","authors":"Torkaman Maryam, Moharrami Mojtaba, Pourtaghi Hadi, Nikbakht Brujeni Gholamreza, Bagheri Masoumeh","doi":"10.32592/ARI.2025.80.4.869","DOIUrl":"https://doi.org/10.32592/ARI.2025.80.4.869","url":null,"abstract":"<p><p>Several agents such as bacteria, fungi, parasites and viruses can infect honey bees in apiculture. Viruses are one of the most important threats to the health of honeybees. The aim of this research was to diagnose common honeybee pathogenic viruses, including Acute Bee Paralysis Virus (ABPV), Black Queen Cell Virus (BQCV), Chronic Bee Paralysis Virus (CBPV, Deformed Wing Defect (DWV), Kashmir Bee Virus (KBV), and Sacbrood Virus (SBV) in apiaries across Iran. From Autumn to Winter 2022, honey bee samples were randomly collected from 31 provinces across the country. After samples preparation and RNA extraction, the target fragments were amplified using the RT-PCR method. Desired standard viruses and distilled water were used as positive and negative controls, respectively. The PCR products were sequenced and compared with the Genbank database. Results showed that, out of 274 samples collected from apiaries, 21 (7.66%), 21 (7.66%), 11 (4.01%), 247 (90.15%), 31 (11.1%) and 91 (33.21%) were positive for ABPV, BQCV, CBPV, DWV, KBV and SBV, respectively. The highest level of infection was related to DWV and SBV, while the lowest level of infection was related to CBPV. All provinces were infected with at least one virus, and in some, all the studied viruses were detected in apiaries. Sequencing results confirmed the RT-PCR findings. Phylogenetic analysis showed several mutations in the sequences of all studied viruses. The presence of viral infections in the country's apiaries indicates that viral diseases are a significant concern and require targeted management strategies for prevention.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"80 4","pages":"869-878"},"PeriodicalIF":0.0,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Susceptibility Patterns and Genetic Relatedness between Diarrheagenic <i>Escherichia coli</i> Pathotypes Isolated from Ready-to-Eat Olivier Salad and Clinical Samples.","authors":"Soltan Dallal Mohammad Mehdi, Karimaei Samira, Nasser Ahmad, Yaslianifard Somayeh","doi":"10.32592/ARI.2025.80.4.969","DOIUrl":"https://doi.org/10.32592/ARI.2025.80.4.969","url":null,"abstract":"<p><p>Diarrheagenic <i>Escherichia coli</i> (DEC) strains are among the most prevalent bacteria transmitted through contaminated water and food, and are related to mild-to-severe diarrhea in humans. The present study aimed to evaluate the prevalence, antibiotic resistance profile, phototypes, and biofilm formation capacity of <i>E. coli</i> isolates obtained from Olivier Salad and clinical samples. A total of 246 samples-including Olivier salad and stool specimens-were collected in Tehran between March to August 2022. Microbiological and molecular diagnostic methods were used to detect DEC strains. Disk diffusion and biofilm formation methods were done to evaluate the antimicrobial resistance profile and biofilm formation capacity of the <i>E. coli</i> isolates. Overall, 16.6% (41/246) of <i>E. coli</i> isolates was attained from both Olivier Salad and clinical samples and the prevalence of DEC among these isolates was 17% (7/41). The identified DEC phototypes from the 41 isolates were as follows: enteropathogenic <i>E. coli</i> (EPEC, 4.8%), and enterotoxigenic <i>E. coli</i> (ETEC, 12.1%), while, no isolates of enteroaggregative <i>E. coli</i> (EAEC), enterinvasive <i>E. coli</i> (EIEC), and enteroaggregative <i>E. coli</i> (EHEC) were found. The highest rate of resistance was found for amoxicillin (100%), and all DEC strains exhibited resistance to at least one antibiotic. Isolates obtained from clinical samples had more biofilm formation capacity than food samples. Our results evidenced the possibility of fecal contamination in animal-derived foods. Also, multi-drug resistances were found between DEC isolated from food that suggested animal-based foods would operate as the reservoir for multi-drug resistant bacteria. Monitoring DEC strains in both food and clinical samples is essential to improve food safety and prevent foodborne outbreaks.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"80 4","pages":"969-977"},"PeriodicalIF":0.0,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of exposure to sericin on serum GH, IGF-1 and antioxidant levels following parturition in mice.","authors":"Fadaei Shahla, Hassanpour Shahin, Eidi Akram, Zendehdel Morteza","doi":"10.32592/ARI.2025.80.4.1059","DOIUrl":"https://doi.org/10.32592/ARI.2025.80.4.1059","url":null,"abstract":"<p><p>This study aimed to determine the effects of exposure to sericin on serum GH, IGF-1 and antioxidant levels following parturition in mice. Forty pregnant female NMRI mice were allocated into four groups. In the control group, pregnant mice were given water as a placebo, whereas pregnant female mice in groups 2-4 were orally administered sericin (112.5, 225, and 450 mg/kg) on different days of gestation (5, 8, 11, 14, and 17). Following parturition, the open field test (OFT), forced swimming test (FST), tail suspension test (TST) and open field tests were used to evaluate depressive-like and antidepressant activity of sericin. At the end of the study, serum growth hormone (GH), insulin-like growth factor-1 (IGF-1), malondialdehyde (MDA), glutathione peroxidase (GPx), superoxide dismutase (SOD), and total antioxidant status (TAS) levels were determined. According to the results, sericin administration during pregnancy decreased immobility time in FST and TST, and increased the number of crosses in the OFT compared to the control group (P<0.05). Exposure to sericin significantly decreased serum MDA, while it increased SOD, GPx, and TAS levels compared with the control group (P<0.05). Also, sericin exposure during gestation significantly increased serum GH and IGF-1 levels compared to the control group (P<0.05). These results suggested pre-partum administration of sericin has antidepressant effect in postpartum mice.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"80 4","pages":"1059-1067"},"PeriodicalIF":0.0,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Recombinant Production of Novel Bovine Lactoferricin Engineered Peptide Using Molecular Dynamics Simulation.","authors":"Akbari Azam Maryam, Motalebi Abbasali, Nassiri Mohammadreza, Anvar Amirali","doi":"10.32592/ARI.2025.80.4.995","DOIUrl":"https://doi.org/10.32592/ARI.2025.80.4.995","url":null,"abstract":"<p><p>Antimicrobial peptides (AMPs) are native and safe short peptides that are considered one of the best alternatives to antibiotics. Although numerous studies have been conducted on AMPs, their mode of action has not yet been fully understood. Computational peptide engineering can provide valuable insight into the stability and potency of AMPs against targets. In the present study, we performed a molecular dynamics simulation to understand the mode of action of Bovine Lfcin and to improve the interactions between Bovine Lfcin (wild and mutant types) and DNA (an important intracellular target), DNAK (an important protein in pathogenicity, mostly in gram-negative bacteria), and LysM (an important surface protein in gram-positive bacteria). The nucleotide sequence of Lfcin peptide was synthesized and cloned into pET22b (+). Induction of gene expression was performed using 1mM IPTG and recombinant peptide was purified using a His-tag marker. The antimicrobial peptide activity was tested on <i>Escherichia coli O157</i> and <i>Bacillus subtilis</i>, Gram- negative and positive bacteria, respectively, using disk diffusion methods. Our results showed that all the changes in the Bovine Lfcin wild type observed in this study improved the peptide-DNA, DNAK, and LysM interactions. Based on the results, removing the PHE25 residue from the wild- type Bovine Lfcin peptide had more significant effects on complex formation with DNA, DNAK, and LysM. The recombinant production of an Lfcin peptide with a molecular weight of ~5 KDa was confirmed by SDS PAGE. The performance of Lfcin peptide on pathogenic bacteria was strong, and it had the strongest effect at concentrations of 6 and 8 mg/ml for Gram- positive and Gram-negative bacteria, respectively.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"80 4","pages":"995-1003"},"PeriodicalIF":0.0,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13125766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}